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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 485 |
| AccB1I | GGYRCC | 1 cut(s) | 94 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 416, 556 |
| AccI | GTMKAC | 1 cut(s) | 467 |
| AciI | CCGC | 1 cut(s) | 113 |
| AclI | AACGTT | 1 cut(s) | 488 |
| AcyI | GRCGYC | 1 cut(s) | 482 |
| AfaI | GTAC | 1 cut(s) | 359 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 52, 300, 416, 428, 556 |
| AgsI | TTSAA | 1 cut(s) | 35 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 425 |
| AjnI | CCWGG | 1 cut(s) | 99 |
| AluBI | AGCT | 4 cut(s) | 220, 229, 283, 582 |
| AluI | AGCT | 4 cut(s) | 220, 229, 283, 582 |
| Alw26I | GTCTC | 1 cut(s) | 182 |
| Ama87I | CYCGRG | 1 cut(s) | 47 |
| AoxI | GGCC | 1 cut(s) | 330 |
| ApeKI | GCWGC | 1 cut(s) | 449 |
| AspS9I | GGNCC | 2 cut(s) | 330, 419 |
| AsuHPI | GGTGA | 2 cut(s) | 197, 490 |
| AvaI | CYCGRG | 1 cut(s) | 47 |
| AvaII | GGWCC | 1 cut(s) | 419 |
| BaeGI | GKGCMC | 2 cut(s) | 74, 99 |
| BanI | GGYRCC | 1 cut(s) | 94 |
| BbsI | GAAGAC | 1 cut(s) | 48 |
| BbvI | GCAGC | 1 cut(s) | 461 |
| BccI | CCATC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 1 cut(s) | 101 |
| BcoDI | GTCTC | 1 cut(s) | 182 |
| BfaI | CTAG | 1 cut(s) | 221 |
| BglII | AGATCT | 2 cut(s) | 268, 310 |
| BisI | GCNGC | 1 cut(s) | 450 |
| BlsI | GCNGC | 1 cut(s) | 451 |
| Bme1390I | CCNGG | 1 cut(s) | 101 |
| Bme18I | GGWCC | 1 cut(s) | 419 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 425 |
| BmeT110I | CYCGRG | 1 cut(s) | 47 |
| BmgT120I | GGNCC | 2 cut(s) | 330, 419 |
| BmiI | GGNNCC | 1 cut(s) | 96 |
| BmrFI | CCNGG | 1 cut(s) | 101 |
| BpiI | GAAGAC | 1 cut(s) | 48 |
| BpmI | CTGGAG | 1 cut(s) | 335 |
| BsaBI | GATNNNNATC | 1 cut(s) | 405 |
| BsaHI | GRCGYC | 1 cut(s) | 482 |
| BsaJI | CCNNGG | 2 cut(s) | 17, 99 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 48, 78 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 52, 300, 416, 428, 556 |
| Bse3DI | GCAATG | 2 cut(s) | 495, 507 |
| Bse8I | GATNNNNATC | 1 cut(s) | 405 |
| BseBI | CCWGG | 1 cut(s) | 101 |
| BseDI | CCNNGG | 2 cut(s) | 17, 99 |
| BseGI | GGATG | 2 cut(s) | 394, 406 |
| BseJI | GATNNNNATC | 1 cut(s) | 405 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 52, 300, 416, 428, 556 |
| BseMI | GCAATG | 2 cut(s) | 495, 507 |
| BseSI | GKGCMC | 2 cut(s) | 74, 99 |
| BseXI | GCAGC | 1 cut(s) | 461 |
| BsgI | GTGCAG | 1 cut(s) | 86 |
| BshFI | GGCC | 1 cut(s) | 332 |
| BshNI | GGYRCC | 1 cut(s) | 94 |
| BsiHKCI | CYCGRG | 1 cut(s) | 47 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 52, 300, 416, 428, 556 |
| BsmAI | GTCTC | 1 cut(s) | 182 |
| BsmI | GAATGC | 1 cut(s) | 259 |
| BsnI | GGCC | 1 cut(s) | 332 |
| BsoBI | CYCGRG | 1 cut(s) | 47 |
| Bsp1286I | GDGCHC | 2 cut(s) | 74, 99 |
| Bsp1407I | TGTACA | 1 cut(s) | 357 |
| Bsp143I | GATC | 2 cut(s) | 268, 310 |
| BspACI | CCGC | 1 cut(s) | 113 |
| BspANI | GGCC | 1 cut(s) | 332 |
| BspLI | GGNNCC | 1 cut(s) | 96 |
| BspT107I | GGYRCC | 1 cut(s) | 94 |
| BsrDI | GCAATG | 2 cut(s) | 495, 507 |
| BsrGI | TGTACA | 1 cut(s) | 357 |
| BssECI | CCNNGG | 2 cut(s) | 17, 99 |
| BssMI | GATC | 2 cut(s) | 268, 310 |
| BssNI | GRCGYC | 1 cut(s) | 482 |
| Bst2UI | CCWGG | 1 cut(s) | 101 |
| Bst4CI | ACNGT | 1 cut(s) | 18 |
| Bst6I | CTCTTC | 1 cut(s) | 111 |
| BstACI | GRCGYC | 1 cut(s) | 482 |
| BstAUI | TGTACA | 1 cut(s) | 357 |
| BstC8I | GCNNGC | 2 cut(s) | 106, 231 |
| BstDSI | CCRYGG | 1 cut(s) | 17 |
| BstF5I | GGATG | 2 cut(s) | 394, 406 |
| BstKTI | GATC | 2 cut(s) | 271, 313 |
| BstMAI | GTCTC | 1 cut(s) | 182 |
| BstMBI | GATC | 2 cut(s) | 268, 310 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 110, 226 |
| BstNI | CCWGG | 1 cut(s) | 101 |
| BstSCI | CCNGG | 1 cut(s) | 99 |
| BstSLI | GKGCMC | 2 cut(s) | 74, 99 |
| BstV1I | GCAGC | 1 cut(s) | 461 |
| BstV2I | GAAGAC | 1 cut(s) | 48 |
| BstX2I | RGATCY | 2 cut(s) | 268, 310 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 208, 502 |
| BstYI | RGATCY | 2 cut(s) | 268, 310 |
| BsuRI | GGCC | 1 cut(s) | 332 |
| BtgI | CCRYGG | 1 cut(s) | 17 |
| BtsCI | GGATG | 2 cut(s) | 394, 406 |
| Cac8I | GCNNGC | 2 cut(s) | 106, 231 |
| Cfr13I | GGNCC | 2 cut(s) | 330, 419 |
| CseI | GACGC | 1 cut(s) | 415 |
| Csp6I | GTAC | 1 cut(s) | 358 |
| CviAII | CATG | 1 cut(s) | 475 |
| CviJI | RGCY | 9 cut(s) | 108, 196, 220, 229, 283, 332, 367, 530, 582 |
| CviKI_1 | RGCY | 9 cut(s) | 108, 196, 220, 229, 283, 332, 367, 530, 582 |
| CviQI | GTAC | 1 cut(s) | 358 |
| DpnI | GATC | 2 cut(s) | 270, 312 |
| DpnII | GATC | 2 cut(s) | 268, 310 |
| DriI | GACNNNNNGTC | 1 cut(s) | 425 |
| Eam1104I | CTCTTC | 1 cut(s) | 111 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 425 |
| EarI | CTCTTC | 1 cut(s) | 111 |
| Eco47I | GGWCC | 1 cut(s) | 419 |
| Eco88I | CYCGRG | 1 cut(s) | 47 |
| EcoRII | CCWGG | 1 cut(s) | 99 |
| FaeI | CATG | 1 cut(s) | 478 |
| FaiI | YATR | 5 cut(s) | 274, 308, 447, 476, 592 |
| FatI | CATG | 1 cut(s) | 474 |
| FblI | GTMKAC | 1 cut(s) | 467 |
| Fnu4HI | GCNGC | 1 cut(s) | 450 |
| FokI | GGATG | 2 cut(s) | 401, 413 |
| Fsp4HI | GCNGC | 1 cut(s) | 450 |
| FspBI | CTAG | 1 cut(s) | 221 |
| GluI | GCNGC | 1 cut(s) | 450 |
| GsuI | CTGGAG | 1 cut(s) | 335 |
| HaeIII | GGCC | 1 cut(s) | 332 |
| HgaI | GACGC | 1 cut(s) | 415 |
| Hin1I | GRCGYC | 1 cut(s) | 482 |
| Hin1II | CATG | 1 cut(s) | 478 |
| HincII | GTYRAC | 2 cut(s) | 468, 486 |
| HindII | GTYRAC | 2 cut(s) | 468, 486 |
| HindIII | AAGCTT | 1 cut(s) | 227 |
| HinfI | GANTC | 1 cut(s) | 561 |
| HphI | GGTGA | 2 cut(s) | 197, 490 |
| Hpy166II | GTNNAC | 2 cut(s) | 468, 486 |
| Hpy188I | TCNGA | 1 cut(s) | 431 |
| Hpy188III | TCNNGA | 2 cut(s) | 314, 565 |
| Hpy8I | GTNNAC | 2 cut(s) | 468, 486 |
| Hpy99I | CGWCG | 2 cut(s) | 431, 472 |
| HpyCH4III | ACNGT | 1 cut(s) | 18 |
| HpyCH4IV | ACGT | 4 cut(s) | 37, 482, 488, 555 |
| HpyCH4V | TGCA | 3 cut(s) | 67, 449, 500 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 110, 226 |
| HpySE526I | ACGT | 4 cut(s) | 37, 482, 488, 555 |
| Hsp92I | GRCGYC | 1 cut(s) | 482 |
| Hsp92II | CATG | 1 cut(s) | 478 |
| Kzo9I | GATC | 2 cut(s) | 268, 310 |
| Lsp1109I | GCAGC | 1 cut(s) | 461 |
| MaeI | CTAG | 1 cut(s) | 221 |
| MaeII | ACGT | 4 cut(s) | 37, 482, 488, 555 |
| MaeIII | GTNAC | 1 cut(s) | 478 |
| MalI | GATC | 2 cut(s) | 270, 312 |
| MboI | GATC | 2 cut(s) | 268, 310 |
| MboII | GAAGA | 4 cut(s) | 53, 128, 271, 583 |
| MflI | RGATCY | 2 cut(s) | 268, 310 |
| MhlI | GDGCHC | 2 cut(s) | 74, 99 |
| MluCI | AATT | 2 cut(s) | 30, 506 |
| MmeI | TCCRAC | 1 cut(s) | 409 |
| MslI | CAYNNNNRTG | 1 cut(s) | 500 |
| MspR9I | CCNGG | 1 cut(s) | 101 |
| Mva1269I | GAATGC | 1 cut(s) | 259 |
| MvaI | CCWGG | 1 cut(s) | 101 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 110, 226 |
| NdeII | GATC | 2 cut(s) | 268, 310 |
| NlaIII | CATG | 1 cut(s) | 478 |
| NlaIV | GGNNCC | 1 cut(s) | 96 |
| NmuCI | GTSAC | 1 cut(s) | 478 |
| PaeR7I | CTCGAG | 1 cut(s) | 47 |
| PctI | GAATGC | 1 cut(s) | 259 |
| PfeI | GAWTC | 1 cut(s) | 561 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 416, 556 |
| PkrI | GCNGC | 1 cut(s) | 451 |
| Psp1406I | AACGTT | 1 cut(s) | 488 |
| Psp6I | CCWGG | 1 cut(s) | 99 |
| PspGI | CCWGG | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 1 cut(s) | 96 |
| PspPI | GGNCC | 2 cut(s) | 330, 419 |
| PspXI | VCTCGAGB | 1 cut(s) | 47 |
| PsuI | RGATCY | 2 cut(s) | 268, 310 |
| RsaI | GTAC | 1 cut(s) | 359 |
| RsaNI | GTAC | 1 cut(s) | 358 |
| RseI | CAYNNNNRTG | 1 cut(s) | 500 |
| SalI | GTCGAC | 1 cut(s) | 466 |
| SatI | GCNGC | 1 cut(s) | 450 |
| Sau3AI | GATC | 2 cut(s) | 268, 310 |
| Sau96I | GGNCC | 2 cut(s) | 330, 419 |
| ScrFI | CCNGG | 1 cut(s) | 101 |
| SduI | GDGCHC | 2 cut(s) | 74, 99 |
| Sfr274I | CTCGAG | 1 cut(s) | 47 |
| SinI | GGWCC | 1 cut(s) | 419 |
| SlaI | CTCGAG | 1 cut(s) | 47 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 500 |
| SmlI | CTYRAG | 1 cut(s) | 47 |
| SmoI | CTYRAG | 1 cut(s) | 47 |
| Sse9I | AATT | 2 cut(s) | 30, 506 |
| SsiI | CCGC | 1 cut(s) | 113 |
| SspMI | CTAG | 1 cut(s) | 221 |
| StyD4I | CCNGG | 1 cut(s) | 99 |
| TaaI | ACNGT | 1 cut(s) | 18 |
| TaiI | ACGT | 4 cut(s) | 40, 485, 491, 558 |
| TaqI | TCGA | 3 cut(s) | 48, 467, 584 |
| TasI | AATT | 2 cut(s) | 30, 506 |
| TatI | WGTACW | 1 cut(s) | 357 |
| TfiI | GAWTC | 1 cut(s) | 561 |
| TseFI | GTSAC | 1 cut(s) | 478 |
| TseI | GCWGC | 1 cut(s) | 449 |
| Tsp45I | GTSAC | 1 cut(s) | 478 |
| TspDTI | ATGAA | 7 cut(s) | 27, 144, 336, 405, 417, 540, 590 |
| TspGWI | ACGGA | 1 cut(s) | 485 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 416, 556 |
| VpaK11BI | GGWCC | 1 cut(s) | 419 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 205 |
| XhoI | CTCGAG | 1 cut(s) | 47 |
| XmiI | GTMKAC | 1 cut(s) | 467 |
| XspI | CTAG | 1 cut(s) | 221 |
| ZraI | GACGTC | 1 cut(s) | 483 |