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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 111 |
| AclWI | GGATC | 1 cut(s) | 677 |
| AcoI | YGGCCR | 1 cut(s) | 539 |
| AcsI | RAATTY | 1 cut(s) | 150 |
| AfaI | GTAC | 3 cut(s) | 163, 440, 533 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 353, 471 |
| AgsI | TTSAA | 3 cut(s) | 65, 367, 385 |
| AluBI | AGCT | 3 cut(s) | 56, 220, 418 |
| AluI | AGCT | 3 cut(s) | 56, 220, 418 |
| Alw21I | GWGCWC | 1 cut(s) | 86 |
| Alw44I | GTGCAC | 1 cut(s) | 82 |
| AlwI | GGATC | 1 cut(s) | 677 |
| Ama87I | CYCGRG | 1 cut(s) | 673 |
| AoxI | GGCC | 1 cut(s) | 539 |
| ApaLI | GTGCAC | 1 cut(s) | 82 |
| ApeKI | GCWGC | 2 cut(s) | 73, 574 |
| ApoI | RAATTY | 1 cut(s) | 150 |
| AspS9I | GGNCC | 1 cut(s) | 519 |
| AsuC2I | CCSGG | 1 cut(s) | 472 |
| AsuHPI | GGTGA | 4 cut(s) | 185, 305, 332, 454 |
| AsuNHI | GCTAGC | 1 cut(s) | 418 |
| AvaI | CYCGRG | 1 cut(s) | 673 |
| AvaII | GGWCC | 1 cut(s) | 519 |
| BaeGI | GKGCMC | 1 cut(s) | 86 |
| BalI | TGGCCA | 1 cut(s) | 541 |
| BanII | GRGCYC | 1 cut(s) | 478 |
| BbsI | GAAGAC | 2 cut(s) | 204, 656 |
| Bbv12I | GWGCWC | 1 cut(s) | 86 |
| BbvI | GCAGC | 2 cut(s) | 85, 561 |
| BccI | CCATC | 2 cut(s) | 530, 559 |
| BceAI | ACGGC | 2 cut(s) | 345, 376 |
| BclI | TGATCA | 1 cut(s) | 432 |
| BcnI | CCSGG | 1 cut(s) | 472 |
| BfaI | CTAG | 1 cut(s) | 419 |
| BisI | GCNGC | 2 cut(s) | 74, 575 |
| BlsI | GCNGC | 2 cut(s) | 75, 576 |
| Bme1390I | CCNGG | 1 cut(s) | 472 |
| Bme18I | GGWCC | 1 cut(s) | 519 |
| BmeT110I | CYCGRG | 1 cut(s) | 673 |
| BmgT120I | GGNCC | 1 cut(s) | 519 |
| BmiI | GGNNCC | 2 cut(s) | 520, 641 |
| BmrFI | CCNGG | 1 cut(s) | 472 |
| BmsI | GCATC | 1 cut(s) | 605 |
| BmtI | GCTAGC | 1 cut(s) | 422 |
| BpiI | GAAGAC | 2 cut(s) | 204, 656 |
| BpmI | CTGGAG | 1 cut(s) | 121 |
| BpuEI | CTTGAG | 1 cut(s) | 665 |
| BpuMI | CCSGG | 1 cut(s) | 472 |
| BsaAI | YACGTR | 1 cut(s) | 438 |
| BsaJI | CCNNGG | 3 cut(s) | 471, 542, 695 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 580, 610, 676, 706 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 353, 471 |
| Bse1I | ACTGG | 2 cut(s) | 641, 671 |
| BseDI | CCNNGG | 3 cut(s) | 471, 542, 695 |
| BseGI | GGATG | 2 cut(s) | 49, 620 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 353, 471 |
| BseMII | CTCAG | 3 cut(s) | 103, 369, 491 |
| BseNI | ACTGG | 2 cut(s) | 641, 671 |
| BseSI | GKGCMC | 1 cut(s) | 86 |
| BseXI | GCAGC | 2 cut(s) | 85, 561 |
| BshFI | GGCC | 1 cut(s) | 541 |
| BsiHKAI | GWGCWC | 1 cut(s) | 86 |
| BsiHKCI | CYCGRG | 1 cut(s) | 673 |
| BsiSI | CCGG | 1 cut(s) | 471 |
| BslFI | GGGAC | 2 cut(s) | 28, 532 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 353, 471 |
| BsmFI | GGGAC | 2 cut(s) | 28, 532 |
| BsnI | GGCC | 1 cut(s) | 541 |
| BsoBI | CYCGRG | 1 cut(s) | 673 |
| Bsp1286I | GDGCHC | 2 cut(s) | 86, 478 |
| Bsp143I | GATC | 3 cut(s) | 180, 432, 669 |
| BspANI | GGCC | 1 cut(s) | 541 |
| BspCNI | CTCAG | 3 cut(s) | 102, 370, 490 |
| BspLI | GGNNCC | 2 cut(s) | 520, 641 |
| BspOI | GCTAGC | 1 cut(s) | 422 |
| BspPI | GGATC | 1 cut(s) | 677 |
| BsrI | ACTGG | 2 cut(s) | 641, 671 |
| BssECI | CCNNGG | 3 cut(s) | 471, 542, 695 |
| BssMI | GATC | 3 cut(s) | 180, 432, 669 |
| BssT1I | CCWWGG | 2 cut(s) | 542, 695 |
| Bst4CI | ACNGT | 2 cut(s) | 502, 596 |
| BstBAI | YACGTR | 1 cut(s) | 438 |
| BstC8I | GCNNGC | 2 cut(s) | 420, 620 |
| BstDEI | CTNAG | 4 cut(s) | 57, 89, 378, 477 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 351 |
| BstF5I | GGATG | 2 cut(s) | 49, 620 |
| BstKTI | GATC | 3 cut(s) | 183, 435, 672 |
| BstMBI | GATC | 3 cut(s) | 180, 432, 669 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 226 |
| BstSCI | CCNGG | 1 cut(s) | 470 |
| BstSLI | GKGCMC | 1 cut(s) | 86 |
| BstV1I | GCAGC | 2 cut(s) | 85, 561 |
| BstV2I | GAAGAC | 2 cut(s) | 204, 656 |
| BstX2I | RGATCY | 1 cut(s) | 669 |
| BstYI | RGATCY | 1 cut(s) | 669 |
| BsuRI | GGCC | 1 cut(s) | 541 |
| BtsCI | GGATG | 2 cut(s) | 49, 620 |
| BtsI | GCAGTG | 1 cut(s) | 429 |
| BtsIMutI | CAGTG | 2 cut(s) | 429, 498 |
| Cac8I | GCNNGC | 2 cut(s) | 420, 620 |
| Cfr13I | GGNCC | 1 cut(s) | 519 |
| CseI | GACGC | 2 cut(s) | 122, 416 |
| Csp6I | GTAC | 3 cut(s) | 162, 439, 532 |
| CviAII | CATG | 2 cut(s) | 230, 486 |
| CviJI | RGCY | 8 cut(s) | 56, 220, 332, 391, 418, 476, 541, 574 |
| CviKI_1 | RGCY | 8 cut(s) | 56, 220, 332, 391, 418, 476, 541, 574 |
| CviQI | GTAC | 3 cut(s) | 162, 439, 532 |
| DdeI | CTNAG | 4 cut(s) | 57, 89, 378, 477 |
| DpnI | GATC | 3 cut(s) | 182, 434, 671 |
| DpnII | GATC | 3 cut(s) | 180, 432, 669 |
| EaeI | YGGCCR | 1 cut(s) | 539 |
| Eco130I | CCWWGG | 2 cut(s) | 542, 695 |
| Eco24I | GRGCYC | 1 cut(s) | 478 |
| Eco47I | GGWCC | 1 cut(s) | 519 |
| Eco88I | CYCGRG | 1 cut(s) | 673 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 351 |
| EcoT14I | CCWWGG | 2 cut(s) | 542, 695 |
| EcoT38I | GRGCYC | 1 cut(s) | 478 |
| ErhI | CCWWGG | 2 cut(s) | 542, 695 |
| FaeI | CATG | 2 cut(s) | 233, 489 |
| FaiI | YATR | 6 cut(s) | 231, 305, 374, 487, 494, 585 |
| FaqI | GGGAC | 2 cut(s) | 28, 532 |
| FatI | CATG | 2 cut(s) | 229, 485 |
| FbaI | TGATCA | 1 cut(s) | 432 |
| FblI | GTMKAC | 1 cut(s) | 111 |
| Fnu4HI | GCNGC | 2 cut(s) | 74, 575 |
| FokI | GGATG | 2 cut(s) | 56, 627 |
| FriOI | GRGCYC | 1 cut(s) | 478 |
| Fsp4HI | GCNGC | 2 cut(s) | 74, 575 |
| FspBI | CTAG | 1 cut(s) | 419 |
| GluI | GCNGC | 2 cut(s) | 74, 575 |
| GsuI | CTGGAG | 1 cut(s) | 121 |
| HaeIII | GGCC | 1 cut(s) | 541 |
| HapII | CCGG | 1 cut(s) | 471 |
| HgaI | GACGC | 2 cut(s) | 122, 416 |
| Hin1II | CATG | 2 cut(s) | 233, 489 |
| HinfI | GANTC | 1 cut(s) | 550 |
| HpaII | CCGG | 1 cut(s) | 471 |
| HphI | GGTGA | 4 cut(s) | 185, 305, 332, 454 |
| Hpy166II | GTNNAC | 3 cut(s) | 84, 112, 509 |
| Hpy188I | TCNGA | 2 cut(s) | 480, 555 |
| Hpy188III | TCNNGA | 2 cut(s) | 100, 562 |
| Hpy8I | GTNNAC | 3 cut(s) | 84, 112, 509 |
| HpyAV | CCTTC | 6 cut(s) | 19, 34, 106, 347, 353, 563 |
| HpyCH4III | ACNGT | 2 cut(s) | 502, 596 |
| HpyCH4IV | ACGT | 2 cut(s) | 437, 452 |
| HpyCH4V | TGCA | 3 cut(s) | 84, 577, 602 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 226 |
| HpyF3I | CTNAG | 4 cut(s) | 57, 89, 378, 477 |
| HpySE526I | ACGT | 2 cut(s) | 437, 452 |
| Hsp92II | CATG | 2 cut(s) | 233, 489 |
| Ksp22I | TGATCA | 1 cut(s) | 432 |
| Kzo9I | GATC | 3 cut(s) | 180, 432, 669 |
| Lsp1109I | GCAGC | 2 cut(s) | 85, 561 |
| LweI | GCATC | 1 cut(s) | 605 |
| MaeI | CTAG | 1 cut(s) | 419 |
| MaeII | ACGT | 2 cut(s) | 437, 452 |
| MaeIII | GTNAC | 1 cut(s) | 496 |
| MalI | GATC | 3 cut(s) | 182, 434, 671 |
| MboI | GATC | 3 cut(s) | 180, 432, 669 |
| MfeI | CAATTG | 2 cut(s) | 280, 535 |
| MflI | RGATCY | 1 cut(s) | 669 |
| MhlI | GDGCHC | 2 cut(s) | 86, 478 |
| MlsI | TGGCCA | 1 cut(s) | 541 |
| MluCI | AATT | 5 cut(s) | 150, 280, 309, 513, 535 |
| MluNI | TGGCCA | 1 cut(s) | 541 |
| MnlI | CCTC | 4 cut(s) | 63, 150, 238, 249 |
| Mox20I | TGGCCA | 1 cut(s) | 541 |
| MscI | TGGCCA | 1 cut(s) | 541 |
| MseI | TTAA | 2 cut(s) | 225, 706 |
| Msp20I | TGGCCA | 1 cut(s) | 541 |
| MspI | CCGG | 1 cut(s) | 471 |
| MspR9I | CCNGG | 1 cut(s) | 472 |
| MunI | CAATTG | 2 cut(s) | 280, 535 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 226 |
| NciI | CCSGG | 1 cut(s) | 472 |
| NdeII | GATC | 3 cut(s) | 180, 432, 669 |
| NheI | GCTAGC | 1 cut(s) | 418 |
| NlaIII | CATG | 2 cut(s) | 233, 489 |
| NlaIV | GGNNCC | 2 cut(s) | 520, 641 |
| NmuCI | GTSAC | 1 cut(s) | 496 |
| PfeI | GAWTC | 1 cut(s) | 550 |
| PkrI | GCNGC | 2 cut(s) | 75, 576 |
| Ppu21I | YACGTR | 1 cut(s) | 438 |
| PspN4I | GGNNCC | 2 cut(s) | 520, 641 |
| PspPI | GGNCC | 1 cut(s) | 519 |
| PsuI | RGATCY | 1 cut(s) | 669 |
| RsaI | GTAC | 3 cut(s) | 163, 440, 533 |
| RsaNI | GTAC | 3 cut(s) | 162, 439, 532 |
| SaqAI | TTAA | 2 cut(s) | 225, 706 |
| SatI | GCNGC | 2 cut(s) | 74, 575 |
| Sau3AI | GATC | 3 cut(s) | 180, 432, 669 |
| Sau96I | GGNCC | 1 cut(s) | 519 |
| ScrFI | CCNGG | 1 cut(s) | 472 |
| SduI | GDGCHC | 2 cut(s) | 86, 478 |
| SfaNI | GCATC | 1 cut(s) | 605 |
| SinI | GGWCC | 1 cut(s) | 519 |
| SmlI | CTYRAG | 1 cut(s) | 644 |
| SmoI | CTYRAG | 1 cut(s) | 644 |
| Sse9I | AATT | 5 cut(s) | 150, 280, 309, 513, 535 |
| SspMI | CTAG | 1 cut(s) | 419 |
| StyD4I | CCNGG | 1 cut(s) | 470 |
| StyI | CCWWGG | 2 cut(s) | 542, 695 |
| TaaI | ACNGT | 2 cut(s) | 502, 596 |
| TaiI | ACGT | 2 cut(s) | 440, 455 |
| TaqI | TCGA | 2 cut(s) | 183, 264 |
| TasI | AATT | 5 cut(s) | 150, 280, 309, 513, 535 |
| TatI | WGTACW | 1 cut(s) | 531 |
| TfiI | GAWTC | 1 cut(s) | 550 |
| Tru1I | TTAA | 2 cut(s) | 225, 706 |
| Tru9I | TTAA | 2 cut(s) | 225, 706 |
| TscAI | CASTG | 2 cut(s) | 429, 505 |
| TseFI | GTSAC | 1 cut(s) | 496 |
| TseI | GCWGC | 2 cut(s) | 73, 574 |
| Tsp45I | GTSAC | 1 cut(s) | 496 |
| TspDTI | ATGAA | 2 cut(s) | 246, 623 |
| TspGWI | ACGGA | 2 cut(s) | 329, 427 |
| TspRI | CASTG | 2 cut(s) | 429, 505 |
| VneI | GTGCAC | 1 cut(s) | 82 |
| VpaK11BI | GGWCC | 1 cut(s) | 519 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 351 |
| XapI | RAATTY | 1 cut(s) | 150 |
| XmiI | GTMKAC | 1 cut(s) | 111 |
| XspI | CTAG | 1 cut(s) | 419 |