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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 65, 236 |
| AatII | GACGTC | 1 cut(s) | 654 |
| AciI | CCGC | 2 cut(s) | 102, 199 |
| AclWI | GGATC | 2 cut(s) | 32, 45 |
| AcoI | YGGCCR | 1 cut(s) | 525 |
| AcuI | CTGAAG | 2 cut(s) | 680, 735 |
| AcyI | GRCGYC | 1 cut(s) | 651 |
| AfaI | GTAC | 1 cut(s) | 71 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 717 |
| AflIII | ACRYGT | 1 cut(s) | 483 |
| AgsI | TTSAA | 6 cut(s) | 116, 149, 410, 559, 644, 862 |
| AluBI | AGCT | 5 cut(s) | 10, 83, 515, 555, 814 |
| AluI | AGCT | 5 cut(s) | 10, 83, 515, 555, 814 |
| Alw26I | GTCTC | 3 cut(s) | 215, 623, 642 |
| AlwI | GGATC | 2 cut(s) | 32, 45 |
| AoxI | GGCC | 1 cut(s) | 525 |
| ApeKI | GCWGC | 3 cut(s) | 80, 361, 364 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 426 |
| AspS9I | GGNCC | 1 cut(s) | 317 |
| AsuHPI | GGTGA | 1 cut(s) | 844 |
| AvaII | GGWCC | 1 cut(s) | 317 |
| BaeGI | GKGCMC | 1 cut(s) | 676 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 119, 152 |
| BamHI | GGATCC | 1 cut(s) | 37 |
| BanII | GRGCYC | 2 cut(s) | 588, 614 |
| BbsI | GAAGAC | 3 cut(s) | 147, 156, 289 |
| BbvI | GCAGC | 3 cut(s) | 92, 373, 376 |
| BccI | CCATC | 3 cut(s) | 234, 319, 776 |
| BceAI | ACGGC | 1 cut(s) | 808 |
| BclI | TGATCA | 1 cut(s) | 508 |
| BcoDI | GTCTC | 3 cut(s) | 215, 623, 642 |
| BfaI | CTAG | 2 cut(s) | 296, 494 |
| BisI | GCNGC | 3 cut(s) | 81, 362, 365 |
| BlsI | GCNGC | 3 cut(s) | 82, 363, 366 |
| Bme18I | GGWCC | 1 cut(s) | 317 |
| BmgT120I | GGNCC | 1 cut(s) | 317 |
| BmiI | GGNNCC | 2 cut(s) | 39, 107 |
| BoxI | GACNNNNGTC | 1 cut(s) | 476 |
| BpiI | GAAGAC | 3 cut(s) | 147, 156, 289 |
| Bpu10I | CCTNAGC | 1 cut(s) | 11 |
| BpuEI | CTTGAG | 2 cut(s) | 116, 572 |
| BsaHI | GRCGYC | 1 cut(s) | 651 |
| BsaJI | CCNNGG | 3 cut(s) | 41, 320, 386 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 717 |
| Bse1I | ACTGG | 4 cut(s) | 173, 425, 637, 854 |
| Bse3DI | GCAATG | 1 cut(s) | 358 |
| BseDI | CCNNGG | 3 cut(s) | 41, 320, 386 |
| BseGI | GGATG | 3 cut(s) | 330, 334, 826 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 717 |
| BseMI | GCAATG | 1 cut(s) | 358 |
| BseNI | ACTGG | 4 cut(s) | 173, 425, 637, 854 |
| BseRI | GAGGAG | 1 cut(s) | 347 |
| BseSI | GKGCMC | 1 cut(s) | 676 |
| BseXI | GCAGC | 3 cut(s) | 92, 373, 376 |
| Bsh1285I | CGRYCG | 1 cut(s) | 474 |
| BshFI | GGCC | 1 cut(s) | 527 |
| BsiEI | CGRYCG | 1 cut(s) | 474 |
| BsiSI | CCGG | 1 cut(s) | 348 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 717 |
| BsmAI | GTCTC | 3 cut(s) | 215, 623, 642 |
| BsmBI | CGTCTC | 1 cut(s) | 642 |
| BsnI | GGCC | 1 cut(s) | 527 |
| Bsp1286I | GDGCHC | 3 cut(s) | 588, 614, 676 |
| Bsp143I | GATC | 3 cut(s) | 37, 255, 508 |
| Bsp19I | CCATGG | 1 cut(s) | 320 |
| BspACI | CCGC | 2 cut(s) | 102, 199 |
| BspANI | GGCC | 1 cut(s) | 527 |
| BspLI | GGNNCC | 2 cut(s) | 39, 107 |
| BspPI | GGATC | 2 cut(s) | 32, 45 |
| BsrDI | GCAATG | 1 cut(s) | 358 |
| BsrI | ACTGG | 4 cut(s) | 173, 425, 637, 854 |
| BssECI | CCNNGG | 3 cut(s) | 41, 320, 386 |
| BssMI | GATC | 3 cut(s) | 37, 255, 508 |
| BssNI | GRCGYC | 1 cut(s) | 651 |
| BssT1I | CCWWGG | 3 cut(s) | 41, 320, 386 |
| Bst4CI | ACNGT | 2 cut(s) | 625, 842 |
| BstACI | GRCGYC | 1 cut(s) | 651 |
| BstC8I | GCNNGC | 2 cut(s) | 576, 729 |
| BstDEI | CTNAG | 1 cut(s) | 11 |
| BstDSI | CCRYGG | 1 cut(s) | 320 |
| BstF5I | GGATG | 3 cut(s) | 330, 334, 826 |
| BstKTI | GATC | 3 cut(s) | 40, 258, 511 |
| BstMAI | GTCTC | 3 cut(s) | 215, 623, 642 |
| BstMBI | GATC | 3 cut(s) | 37, 255, 508 |
| BstMCI | CGRYCG | 1 cut(s) | 474 |
| BstNSI | RCATGY | 1 cut(s) | 487 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 476 |
| BstSLI | GKGCMC | 1 cut(s) | 676 |
| BstV1I | GCAGC | 3 cut(s) | 92, 373, 376 |
| BstV2I | GAAGAC | 3 cut(s) | 147, 156, 289 |
| BstX2I | RGATCY | 1 cut(s) | 37 |
| BstYI | RGATCY | 1 cut(s) | 37 |
| BsuRI | GGCC | 1 cut(s) | 527 |
| BtgI | CCRYGG | 1 cut(s) | 320 |
| BtsCI | GGATG | 3 cut(s) | 330, 334, 826 |
| BtsIMutI | CAGTG | 2 cut(s) | 798, 838 |
| Cac8I | GCNNGC | 2 cut(s) | 576, 729 |
| Cfr13I | GGNCC | 1 cut(s) | 317 |
| Csp6I | GTAC | 1 cut(s) | 70 |
| CviAII | CATG | 3 cut(s) | 321, 484, 807 |
| CviQI | GTAC | 1 cut(s) | 70 |
| DdeI | CTNAG | 1 cut(s) | 11 |
| DpnI | GATC | 3 cut(s) | 39, 257, 510 |
| DpnII | GATC | 3 cut(s) | 37, 255, 508 |
| EaeI | YGGCCR | 1 cut(s) | 525 |
| Eco130I | CCWWGG | 3 cut(s) | 41, 320, 386 |
| Eco24I | GRGCYC | 2 cut(s) | 588, 614 |
| Eco47I | GGWCC | 1 cut(s) | 317 |
| Eco57I | CTGAAG | 2 cut(s) | 680, 735 |
| EcoT14I | CCWWGG | 3 cut(s) | 41, 320, 386 |
| EcoT38I | GRGCYC | 2 cut(s) | 588, 614 |
| ErhI | CCWWGG | 3 cut(s) | 41, 320, 386 |
| Esp3I | CGTCTC | 1 cut(s) | 642 |
| FaeI | CATG | 3 cut(s) | 324, 487, 810 |
| FatI | CATG | 3 cut(s) | 320, 483, 806 |
| FauI | CCCGC | 1 cut(s) | 95 |
| FbaI | TGATCA | 1 cut(s) | 508 |
| Fnu4HI | GCNGC | 3 cut(s) | 81, 362, 365 |
| FokI | GGATG | 3 cut(s) | 337, 341, 833 |
| FriOI | GRGCYC | 2 cut(s) | 588, 614 |
| Fsp4HI | GCNGC | 3 cut(s) | 81, 362, 365 |
| FspBI | CTAG | 2 cut(s) | 296, 494 |
| GluI | GCNGC | 3 cut(s) | 81, 362, 365 |
| HaeIII | GGCC | 1 cut(s) | 527 |
| HapII | CCGG | 1 cut(s) | 348 |
| Hin1I | GRCGYC | 1 cut(s) | 651 |
| Hin1II | CATG | 3 cut(s) | 324, 487, 810 |
| HincII | GTYRAC | 2 cut(s) | 481, 655 |
| HindII | GTYRAC | 2 cut(s) | 481, 655 |
| HinfI | GANTC | 3 cut(s) | 19, 223, 835 |
| HpaII | CCGG | 1 cut(s) | 348 |
| HphI | GGTGA | 1 cut(s) | 844 |
| Hpy166II | GTNNAC | 3 cut(s) | 481, 655, 790 |
| Hpy188I | TCNGA | 1 cut(s) | 24 |
| Hpy188III | TCNNGA | 3 cut(s) | 208, 449, 589 |
| Hpy8I | GTNNAC | 3 cut(s) | 481, 655, 790 |
| HpyAV | CCTTC | 5 cut(s) | 25, 211, 308, 601, 710 |
| HpyCH4III | ACNGT | 2 cut(s) | 625, 842 |
| HpyCH4IV | ACGT | 1 cut(s) | 651 |
| HpyCH4V | TGCA | 1 cut(s) | 436 |
| HpyF3I | CTNAG | 1 cut(s) | 11 |
| HpySE526I | ACGT | 1 cut(s) | 651 |
| Hsp92I | GRCGYC | 1 cut(s) | 651 |
| Hsp92II | CATG | 3 cut(s) | 324, 487, 810 |
| Ksp22I | TGATCA | 1 cut(s) | 508 |
| Kzo9I | GATC | 3 cut(s) | 37, 255, 508 |
| LmnI | GCTCC | 1 cut(s) | 751 |
| Lsp1109I | GCAGC | 3 cut(s) | 92, 373, 376 |
| MaeI | CTAG | 2 cut(s) | 296, 494 |
| MaeII | ACGT | 1 cut(s) | 651 |
| MaeIII | GTNAC | 2 cut(s) | 171, 466 |
| MalI | GATC | 3 cut(s) | 39, 257, 510 |
| MboI | GATC | 3 cut(s) | 37, 255, 508 |
| MfeI | CAATTG | 1 cut(s) | 393 |
| MflI | RGATCY | 1 cut(s) | 37 |
| MhlI | GDGCHC | 3 cut(s) | 588, 614, 676 |
| MluCI | AATT | 2 cut(s) | 290, 393 |
| MlyI | GAGTC | 1 cut(s) | 217 |
| MnlI | CCTC | 7 cut(s) | 64, 292, 325, 328, 575, 675, 739 |
| MroXI | GAANNNNTTC | 1 cut(s) | 426 |
| MspI | CCGG | 1 cut(s) | 348 |
| MunI | CAATTG | 1 cut(s) | 393 |
| NcoI | CCATGG | 1 cut(s) | 320 |
| NdeII | GATC | 3 cut(s) | 37, 255, 508 |
| NlaIII | CATG | 3 cut(s) | 324, 487, 810 |
| NlaIV | GGNNCC | 2 cut(s) | 39, 107 |
| NmuCI | GTSAC | 2 cut(s) | 171, 466 |
| NspI | RCATGY | 1 cut(s) | 487 |
| PciI | ACATGT | 1 cut(s) | 483 |
| PdmI | GAANNNNTTC | 1 cut(s) | 426 |
| PfeI | GAWTC | 2 cut(s) | 19, 835 |
| PkrI | GCNGC | 3 cut(s) | 82, 363, 366 |
| PleI | GAGTC | 1 cut(s) | 217 |
| PpsI | GAGTC | 1 cut(s) | 217 |
| PscI | ACATGT | 1 cut(s) | 483 |
| PshAI | GACNNNNGTC | 1 cut(s) | 476 |
| PsiI | TTATAA | 2 cut(s) | 65, 236 |
| PspN4I | GGNNCC | 2 cut(s) | 39, 107 |
| PspPI | GGNCC | 1 cut(s) | 317 |
| PsuI | RGATCY | 1 cut(s) | 37 |
| RsaI | GTAC | 1 cut(s) | 71 |
| RsaNI | GTAC | 1 cut(s) | 70 |
| SatI | GCNGC | 3 cut(s) | 81, 362, 365 |
| Sau3AI | GATC | 3 cut(s) | 37, 255, 508 |
| Sau96I | GGNCC | 1 cut(s) | 317 |
| SchI | GAGTC | 1 cut(s) | 217 |
| SduI | GDGCHC | 3 cut(s) | 588, 614, 676 |
| SinI | GGWCC | 1 cut(s) | 317 |
| SmlI | CTYRAG | 2 cut(s) | 131, 587 |
| SmoI | CTYRAG | 2 cut(s) | 131, 587 |
| Sse9I | AATT | 2 cut(s) | 290, 393 |
| SsiI | CCGC | 2 cut(s) | 102, 199 |
| SspMI | CTAG | 2 cut(s) | 296, 494 |
| StyI | CCWWGG | 3 cut(s) | 41, 320, 386 |
| TaaI | ACNGT | 2 cut(s) | 625, 842 |
| TaiI | ACGT | 1 cut(s) | 654 |
| TaqI | TCGA | 2 cut(s) | 156, 638 |
| TaqII | GACCGA | 1 cut(s) | 488 |
| TasI | AATT | 2 cut(s) | 290, 393 |
| TfiI | GAWTC | 2 cut(s) | 19, 835 |
| TscAI | CASTG | 2 cut(s) | 805, 845 |
| TseFI | GTSAC | 2 cut(s) | 171, 466 |
| TseI | GCWGC | 3 cut(s) | 80, 361, 364 |
| Tsp45I | GTSAC | 2 cut(s) | 171, 466 |
| TspDTI | ATGAA | 4 cut(s) | 474, 554, 558, 837 |
| TspGWI | ACGGA | 1 cut(s) | 420 |
| TspRI | CASTG | 2 cut(s) | 805, 845 |
| VpaK11BI | GGWCC | 1 cut(s) | 317 |
| XceI | RCATGY | 1 cut(s) | 487 |
| XmnI | GAANNNNTTC | 1 cut(s) | 426 |
| XspI | CTAG | 2 cut(s) | 296, 494 |
| ZraI | GACGTC | 1 cut(s) | 652 |