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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 756 |
| AclWI | GGATC | 1 cut(s) | 350 |
| AcuI | CTGAAG | 2 cut(s) | 144, 227 |
| AcyI | GRCGYC | 1 cut(s) | 257 |
| AfaI | GTAC | 1 cut(s) | 809 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 58, 350 |
| AgsI | TTSAA | 2 cut(s) | 385, 782 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 164, 196 |
| AluBI | AGCT | 5 cut(s) | 167, 233, 308, 482, 736 |
| AluI | AGCT | 5 cut(s) | 167, 233, 308, 482, 736 |
| Alw21I | GWGCWC | 1 cut(s) | 169 |
| Alw26I | GTCTC | 1 cut(s) | 458 |
| AlwI | GGATC | 1 cut(s) | 350 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 736 |
| ApeKI | GCWGC | 3 cut(s) | 479, 769, 772 |
| AspLEI | GCGC | 1 cut(s) | 148 |
| AsuHPI | GGTGA | 1 cut(s) | 365 |
| BanII | GRGCYC | 1 cut(s) | 169 |
| BbsI | GAAGAC | 1 cut(s) | 23 |
| Bbv12I | GWGCWC | 1 cut(s) | 169 |
| BbvI | GCAGC | 3 cut(s) | 491, 781, 784 |
| BccI | CCATC | 3 cut(s) | 241, 269, 859 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 468, 502 |
| BclI | TGATCA | 1 cut(s) | 223 |
| BcoDI | GTCTC | 1 cut(s) | 458 |
| BfmI | CTRYAG | 2 cut(s) | 432, 749 |
| BfuAI | ACCTGC | 1 cut(s) | 756 |
| BglII | AGATCT | 1 cut(s) | 657 |
| BisI | GCNGC | 3 cut(s) | 480, 770, 773 |
| BlsI | GCNGC | 3 cut(s) | 481, 771, 774 |
| BpiI | GAAGAC | 1 cut(s) | 23 |
| BpmI | CTGGAG | 1 cut(s) | 636 |
| BsaHI | GRCGYC | 1 cut(s) | 257 |
| BsaJI | CCNNGG | 1 cut(s) | 344 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 58, 350 |
| Bse1I | ACTGG | 2 cut(s) | 329, 359 |
| BseDI | CCNNGG | 1 cut(s) | 344 |
| BseGI | GGATG | 1 cut(s) | 634 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 58, 350 |
| BseNI | ACTGG | 2 cut(s) | 329, 359 |
| BseRI | GAGGAG | 2 cut(s) | 428, 431 |
| BseXI | GCAGC | 3 cut(s) | 491, 781, 784 |
| BsgI | GTGCAG | 1 cut(s) | 788 |
| BsiHKAI | GWGCWC | 1 cut(s) | 169 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 58, 350 |
| BsmAI | GTCTC | 1 cut(s) | 458 |
| BsmI | GAATGC | 1 cut(s) | 571 |
| Bsp1286I | GDGCHC | 1 cut(s) | 169 |
| Bsp143I | GATC | 3 cut(s) | 223, 355, 657 |
| BspMAI | CTGCAG | 1 cut(s) | 753 |
| BspMI | ACCTGC | 1 cut(s) | 756 |
| BspPI | GGATC | 1 cut(s) | 350 |
| BsrI | ACTGG | 2 cut(s) | 329, 359 |
| BssECI | CCNNGG | 1 cut(s) | 344 |
| BssMI | GATC | 3 cut(s) | 223, 355, 657 |
| BssNI | GRCGYC | 1 cut(s) | 257 |
| BssT1I | CCWWGG | 1 cut(s) | 344 |
| BstACI | GRCGYC | 1 cut(s) | 257 |
| BstC8I | GCNNGC | 2 cut(s) | 255, 738 |
| BstDEI | CTNAG | 1 cut(s) | 618 |
| BstF5I | GGATG | 1 cut(s) | 634 |
| BstHHI | GCGC | 1 cut(s) | 148 |
| BstKTI | GATC | 3 cut(s) | 226, 358, 660 |
| BstMAI | GTCTC | 1 cut(s) | 458 |
| BstMBI | GATC | 3 cut(s) | 223, 355, 657 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 437, 488, 497 |
| BstSFI | CTRYAG | 2 cut(s) | 432, 749 |
| BstV1I | GCAGC | 3 cut(s) | 491, 781, 784 |
| BstV2I | GAAGAC | 1 cut(s) | 23 |
| BstX2I | RGATCY | 1 cut(s) | 657 |
| BstYI | RGATCY | 1 cut(s) | 657 |
| BtsCI | GGATG | 1 cut(s) | 634 |
| BveI | ACCTGC | 1 cut(s) | 756 |
| Cac8I | GCNNGC | 2 cut(s) | 255, 738 |
| CaiI | CAGNNNCTG | 1 cut(s) | 736 |
| CfoI | GCGC | 1 cut(s) | 148 |
| CseI | GACGC | 2 cut(s) | 139, 246 |
| Csp6I | GTAC | 1 cut(s) | 808 |
| CviAII | CATG | 2 cut(s) | 544, 568 |
| CviQI | GTAC | 1 cut(s) | 808 |
| DdeI | CTNAG | 1 cut(s) | 618 |
| DpnI | GATC | 3 cut(s) | 225, 357, 659 |
| DpnII | GATC | 3 cut(s) | 223, 355, 657 |
| Ecl136II | GAGCTC | 1 cut(s) | 167 |
| Eco130I | CCWWGG | 1 cut(s) | 344 |
| Eco24I | GRGCYC | 1 cut(s) | 169 |
| Eco53kI | GAGCTC | 1 cut(s) | 167 |
| Eco57I | CTGAAG | 2 cut(s) | 144, 227 |
| EcoICRI | GAGCTC | 1 cut(s) | 167 |
| EcoT14I | CCWWGG | 1 cut(s) | 344 |
| EcoT22I | ATGCAT | 1 cut(s) | 573 |
| EcoT38I | GRGCYC | 1 cut(s) | 169 |
| ErhI | CCWWGG | 1 cut(s) | 344 |
| FaeI | CATG | 2 cut(s) | 547, 571 |
| FaiI | YATR | 9 cut(s) | 54, 80, 107, 288, 294, 455, 545, 569, 645 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 8, 40 |
| FatI | CATG | 2 cut(s) | 543, 567 |
| FbaI | TGATCA | 1 cut(s) | 223 |
| Fnu4HI | GCNGC | 3 cut(s) | 480, 770, 773 |
| FokI | GGATG | 1 cut(s) | 641 |
| FriOI | GRGCYC | 1 cut(s) | 169 |
| Fsp4HI | GCNGC | 3 cut(s) | 480, 770, 773 |
| GlaI | GCGC | 1 cut(s) | 147 |
| GluI | GCNGC | 3 cut(s) | 480, 770, 773 |
| GsuI | CTGGAG | 1 cut(s) | 636 |
| HgaI | GACGC | 2 cut(s) | 139, 246 |
| HhaI | GCGC | 1 cut(s) | 148 |
| Hin1I | GRCGYC | 1 cut(s) | 257 |
| Hin1II | CATG | 2 cut(s) | 547, 571 |
| Hin6I | GCGC | 1 cut(s) | 146 |
| HinP1I | GCGC | 1 cut(s) | 146 |
| HincII | GTYRAC | 2 cut(s) | 334, 364 |
| HindII | GTYRAC | 2 cut(s) | 334, 364 |
| HpaI | GTTAAC | 1 cut(s) | 364 |
| HphI | GGTGA | 1 cut(s) | 365 |
| Hpy166II | GTNNAC | 3 cut(s) | 334, 364, 810 |
| Hpy188I | TCNGA | 4 cut(s) | 164, 171, 228, 246 |
| Hpy188III | TCNNGA | 5 cut(s) | 385, 463, 473, 653, 661 |
| Hpy8I | GTNNAC | 3 cut(s) | 334, 364, 810 |
| HpyAV | CCTTC | 1 cut(s) | 547 |
| HpyCH4IV | ACGT | 1 cut(s) | 588 |
| HpyCH4V | TGCA | 3 cut(s) | 571, 751, 769 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 437, 488, 497 |
| HpyF3I | CTNAG | 1 cut(s) | 618 |
| HpySE526I | ACGT | 1 cut(s) | 588 |
| Hsp92I | GRCGYC | 1 cut(s) | 257 |
| Hsp92II | CATG | 2 cut(s) | 547, 571 |
| HspAI | GCGC | 1 cut(s) | 146 |
| Ksp22I | TGATCA | 1 cut(s) | 223 |
| KspAI | GTTAAC | 1 cut(s) | 364 |
| Kzo9I | GATC | 3 cut(s) | 223, 355, 657 |
| LmnI | GCTCC | 1 cut(s) | 265 |
| LpnPI | CCDG | 9 cut(s) | 219, 239, 342, 372, 519, 666, 722, 761, 843 |
| Lsp1109I | GCAGC | 3 cut(s) | 491, 781, 784 |
| MaeII | ACGT | 1 cut(s) | 588 |
| MaeIII | GTNAC | 2 cut(s) | 118, 127 |
| MalI | GATC | 3 cut(s) | 225, 357, 659 |
| MboI | GATC | 3 cut(s) | 223, 355, 657 |
| MboII | GAAGA | 7 cut(s) | 17, 28, 124, 211, 284, 350, 523 |
| MflI | RGATCY | 1 cut(s) | 657 |
| MhlI | GDGCHC | 1 cut(s) | 169 |
| MluCI | AATT | 9 cut(s) | 176, 456, 468, 484, 777, 783, 796, 854, 877 |
| MnlI | CCTC | 9 cut(s) | 205, 330, 344, 384, 391, 406, 409, 576, 625 |
| Mph1103I | ATGCAT | 1 cut(s) | 573 |
| MseI | TTAA | 4 cut(s) | 57, 363, 786, 824 |
| MslI | CAYNNNNRTG | 1 cut(s) | 548 |
| Mva1269I | GAATGC | 1 cut(s) | 571 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 437, 488, 497 |
| NdeII | GATC | 3 cut(s) | 223, 355, 657 |
| NlaIII | CATG | 2 cut(s) | 547, 571 |
| NmuCI | GTSAC | 2 cut(s) | 118, 127 |
| NsiI | ATGCAT | 1 cut(s) | 573 |
| PctI | GAATGC | 1 cut(s) | 571 |
| PkrI | GCNGC | 3 cut(s) | 481, 771, 774 |
| Psp124BI | GAGCTC | 1 cut(s) | 169 |
| PstI | CTGCAG | 1 cut(s) | 753 |
| PstNI | CAGNNNCTG | 1 cut(s) | 736 |
| PsuI | RGATCY | 1 cut(s) | 657 |
| RsaI | GTAC | 1 cut(s) | 809 |
| RsaNI | GTAC | 1 cut(s) | 808 |
| RseI | CAYNNNNRTG | 1 cut(s) | 548 |
| SacI | GAGCTC | 1 cut(s) | 169 |
| SaqAI | TTAA | 4 cut(s) | 57, 363, 786, 824 |
| SatI | GCNGC | 3 cut(s) | 480, 770, 773 |
| Sau3AI | GATC | 3 cut(s) | 223, 355, 657 |
| SduI | GDGCHC | 1 cut(s) | 169 |
| SfcI | CTRYAG | 2 cut(s) | 432, 749 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 548 |
| Sse9I | AATT | 9 cut(s) | 176, 456, 468, 484, 777, 783, 796, 854, 877 |
| SstI | GAGCTC | 1 cut(s) | 169 |
| StyI | CCWWGG | 1 cut(s) | 344 |
| TaiI | ACGT | 1 cut(s) | 591 |
| TaqI | TCGA | 3 cut(s) | 462, 474, 870 |
| TasI | AATT | 9 cut(s) | 176, 456, 468, 484, 777, 783, 796, 854, 877 |
| TatI | WGTACW | 1 cut(s) | 807 |
| Tru1I | TTAA | 4 cut(s) | 57, 363, 786, 824 |
| Tru9I | TTAA | 4 cut(s) | 57, 363, 786, 824 |
| TseFI | GTSAC | 2 cut(s) | 118, 127 |
| TseI | GCWGC | 3 cut(s) | 479, 769, 772 |
| Tsp45I | GTSAC | 2 cut(s) | 118, 127 |
| TspDTI | ATGAA | 3 cut(s) | 29, 303, 560 |
| TspGWI | ACGGA | 1 cut(s) | 687 |
| Zsp2I | ATGCAT | 1 cut(s) | 573 |