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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 546 |
| Acc36I | ACCTGC | 1 cut(s) | 474 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 476 |
| AciI | CCGC | 1 cut(s) | 641 |
| AclWI | GGATC | 1 cut(s) | 298 |
| AcoI | YGGCCR | 1 cut(s) | 308 |
| AcuI | CTGAAG | 3 cut(s) | 398, 664, 779 |
| AcyI | GRCGYC | 1 cut(s) | 543 |
| AfaI | GTAC | 3 cut(s) | 283, 383, 659 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 140, 262, 476, 705 |
| AgsI | TTSAA | 4 cut(s) | 485, 534, 667, 754 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 30 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 576, 608 |
| AluBI | AGCT | 4 cut(s) | 328, 343, 604, 750 |
| AluI | AGCT | 4 cut(s) | 328, 343, 604, 750 |
| Alw21I | GWGCWC | 2 cut(s) | 510, 606 |
| Alw26I | GTCTC | 1 cut(s) | 240 |
| AlwI | GGATC | 1 cut(s) | 298 |
| AoxI | GGCC | 2 cut(s) | 308, 357 |
| ApeKI | GCWGC | 1 cut(s) | 834 |
| AspS9I | GGNCC | 1 cut(s) | 709 |
| AvaII | GGWCC | 1 cut(s) | 709 |
| BanII | GRGCYC | 1 cut(s) | 606 |
| BbsI | GAAGAC | 2 cut(s) | 450, 735 |
| Bbv12I | GWGCWC | 2 cut(s) | 510, 606 |
| BbvI | GCAGC | 1 cut(s) | 821 |
| BccI | CCATC | 3 cut(s) | 760, 778, 782 |
| BcoDI | GTCTC | 1 cut(s) | 240 |
| BfaI | CTAG | 1 cut(s) | 734 |
| BfuAI | ACCTGC | 1 cut(s) | 474 |
| BisI | GCNGC | 2 cut(s) | 642, 835 |
| BlsI | GCNGC | 2 cut(s) | 643, 836 |
| BmcAI | AGTACT | 2 cut(s) | 283, 383 |
| Bme18I | GGWCC | 1 cut(s) | 709 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 30 |
| BmgT120I | GGNCC | 1 cut(s) | 709 |
| BmiI | GGNNCC | 1 cut(s) | 710 |
| BmsI | GCATC | 1 cut(s) | 536 |
| BoxI | GACNNNNGTC | 1 cut(s) | 55 |
| BpiI | GAAGAC | 2 cut(s) | 450, 735 |
| BpmI | CTGGAG | 1 cut(s) | 244 |
| BpuEI | CTTGAG | 1 cut(s) | 494 |
| BsaHI | GRCGYC | 1 cut(s) | 543 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 140, 262, 476, 705 |
| Bse1I | ACTGG | 1 cut(s) | 481 |
| BseGI | GGATG | 4 cut(s) | 79, 306, 793, 814 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 140, 262, 476, 705 |
| BseMII | CTCAG | 1 cut(s) | 353 |
| BseNI | ACTGG | 1 cut(s) | 481 |
| BseXI | GCAGC | 1 cut(s) | 821 |
| BsgI | GTGCAG | 1 cut(s) | 211 |
| BshFI | GGCC | 2 cut(s) | 310, 359 |
| BsiHKAI | GWGCWC | 2 cut(s) | 510, 606 |
| BsiSI | CCGG | 1 cut(s) | 307 |
| BslFI | GGGAC | 1 cut(s) | 722 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 140, 262, 476, 705 |
| BsmAI | GTCTC | 1 cut(s) | 240 |
| BsmFI | GGGAC | 1 cut(s) | 722 |
| BsmI | GAATGC | 1 cut(s) | 671 |
| BsnI | GGCC | 2 cut(s) | 310, 359 |
| Bsp1286I | GDGCHC | 2 cut(s) | 510, 606 |
| Bsp143I | GATC | 4 cut(s) | 183, 303, 490, 811 |
| BspACI | CCGC | 1 cut(s) | 641 |
| BspANI | GGCC | 2 cut(s) | 310, 359 |
| BspCNI | CTCAG | 1 cut(s) | 352 |
| BspLI | GGNNCC | 1 cut(s) | 710 |
| BspMI | ACCTGC | 1 cut(s) | 474 |
| BspPI | GGATC | 1 cut(s) | 298 |
| BsrI | ACTGG | 1 cut(s) | 481 |
| BssMI | GATC | 4 cut(s) | 183, 303, 490, 811 |
| BssNI | GRCGYC | 1 cut(s) | 543 |
| Bst4CI | ACNGT | 3 cut(s) | 57, 289, 565 |
| Bst6I | CTCTTC | 1 cut(s) | 683 |
| BstACI | GRCGYC | 1 cut(s) | 543 |
| BstC8I | GCNNGC | 1 cut(s) | 194 |
| BstDEI | CTNAG | 2 cut(s) | 339, 494 |
| BstF5I | GGATG | 4 cut(s) | 79, 306, 793, 814 |
| BstKTI | GATC | 4 cut(s) | 186, 306, 493, 814 |
| BstMAI | GTCTC | 1 cut(s) | 240 |
| BstMBI | GATC | 4 cut(s) | 183, 303, 490, 811 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 466 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 55 |
| BstV1I | GCAGC | 1 cut(s) | 821 |
| BstV2I | GAAGAC | 2 cut(s) | 450, 735 |
| BsuRI | GGCC | 2 cut(s) | 310, 359 |
| BtsCI | GGATG | 4 cut(s) | 79, 306, 793, 814 |
| BtsIMutI | CAGTG | 2 cut(s) | 330, 644 |
| BveI | ACCTGC | 1 cut(s) | 474 |
| Cac8I | GCNNGC | 1 cut(s) | 194 |
| Cfr13I | GGNCC | 1 cut(s) | 709 |
| Csp6I | GTAC | 3 cut(s) | 282, 382, 658 |
| CviAII | CATG | 3 cut(s) | 353, 655, 705 |
| CviQI | GTAC | 3 cut(s) | 282, 382, 658 |
| DdeI | CTNAG | 2 cut(s) | 339, 494 |
| DpnI | GATC | 4 cut(s) | 185, 305, 492, 813 |
| DpnII | GATC | 4 cut(s) | 183, 303, 490, 811 |
| DriI | GACNNNNNGTC | 1 cut(s) | 30 |
| EaeI | YGGCCR | 1 cut(s) | 308 |
| Eam1104I | CTCTTC | 1 cut(s) | 683 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 30 |
| EarI | CTCTTC | 1 cut(s) | 683 |
| Ecl136II | GAGCTC | 1 cut(s) | 604 |
| Eco24I | GRGCYC | 1 cut(s) | 606 |
| Eco47I | GGWCC | 1 cut(s) | 709 |
| Eco53kI | GAGCTC | 1 cut(s) | 604 |
| Eco57I | CTGAAG | 3 cut(s) | 398, 664, 779 |
| EcoICRI | GAGCTC | 1 cut(s) | 604 |
| EcoO109I | RGGNCCY | 1 cut(s) | 709 |
| EcoT38I | GRGCYC | 1 cut(s) | 606 |
| FaeI | CATG | 3 cut(s) | 356, 658, 708 |
| FaqI | GGGAC | 1 cut(s) | 722 |
| FatI | CATG | 3 cut(s) | 352, 654, 704 |
| Fnu4HI | GCNGC | 2 cut(s) | 642, 835 |
| FokI | GGATG | 4 cut(s) | 86, 313, 800, 801 |
| FriOI | GRGCYC | 1 cut(s) | 606 |
| Fsp4HI | GCNGC | 2 cut(s) | 642, 835 |
| FspBI | CTAG | 1 cut(s) | 734 |
| GluI | GCNGC | 2 cut(s) | 642, 835 |
| GsuI | CTGGAG | 1 cut(s) | 244 |
| HaeIII | GGCC | 2 cut(s) | 310, 359 |
| HapII | CCGG | 1 cut(s) | 307 |
| Hin1I | GRCGYC | 1 cut(s) | 543 |
| Hin1II | CATG | 3 cut(s) | 356, 658, 708 |
| HincII | GTYRAC | 1 cut(s) | 822 |
| HindII | GTYRAC | 1 cut(s) | 822 |
| HpaII | CCGG | 1 cut(s) | 307 |
| Hpy166II | GTNNAC | 1 cut(s) | 822 |
| Hpy188I | TCNGA | 3 cut(s) | 61, 783, 798 |
| Hpy188III | TCNNGA | 4 cut(s) | 22, 223, 319, 581 |
| Hpy8I | GTNNAC | 1 cut(s) | 822 |
| HpyAV | CCTTC | 4 cut(s) | 141, 496, 613, 851 |
| HpyCH4III | ACNGT | 3 cut(s) | 57, 289, 565 |
| HpyCH4IV | ACGT | 1 cut(s) | 543 |
| HpyCH4V | TGCA | 3 cut(s) | 179, 192, 207 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 466 |
| HpyF3I | CTNAG | 2 cut(s) | 339, 494 |
| HpySE526I | ACGT | 1 cut(s) | 543 |
| Hsp92I | GRCGYC | 1 cut(s) | 543 |
| Hsp92II | CATG | 3 cut(s) | 356, 658, 708 |
| Kzo9I | GATC | 4 cut(s) | 183, 303, 490, 811 |
| LmnI | GCTCC | 1 cut(s) | 601 |
| LpnPI | CCDG | 8 cut(s) | 35, 178, 208, 320, 462, 479, 566, 816 |
| Lsp1109I | GCAGC | 1 cut(s) | 821 |
| LweI | GCATC | 1 cut(s) | 536 |
| MaeI | CTAG | 1 cut(s) | 734 |
| MaeII | ACGT | 1 cut(s) | 543 |
| MalI | GATC | 4 cut(s) | 185, 305, 492, 813 |
| MboI | GATC | 4 cut(s) | 183, 303, 490, 811 |
| MboII | GAAGA | 8 cut(s) | 106, 334, 455, 522, 625, 628, 670, 740 |
| MfeI | CAATTG | 1 cut(s) | 754 |
| MhlI | GDGCHC | 2 cut(s) | 510, 606 |
| MluCI | AATT | 3 cut(s) | 455, 636, 754 |
| MnlI | CCTC | 9 cut(s) | 256, 291, 349, 508, 546, 686, 722, 792, 848 |
| MseI | TTAA | 3 cut(s) | 99, 393, 701 |
| MslI | CAYNNNNRTG | 1 cut(s) | 351 |
| MspI | CCGG | 1 cut(s) | 307 |
| MunI | CAATTG | 1 cut(s) | 754 |
| Mva1269I | GAATGC | 1 cut(s) | 671 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 466 |
| NdeII | GATC | 4 cut(s) | 183, 303, 490, 811 |
| NlaIII | CATG | 3 cut(s) | 356, 658, 708 |
| NlaIV | GGNNCC | 1 cut(s) | 710 |
| PctI | GAATGC | 1 cut(s) | 671 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 476 |
| PkrI | GCNGC | 2 cut(s) | 643, 836 |
| PpuMI | RGGWCCY | 1 cut(s) | 709 |
| PshAI | GACNNNNGTC | 1 cut(s) | 55 |
| Psp124BI | GAGCTC | 1 cut(s) | 606 |
| Psp5II | RGGWCCY | 1 cut(s) | 709 |
| PspN4I | GGNNCC | 1 cut(s) | 710 |
| PspPI | GGNCC | 1 cut(s) | 709 |
| PspPPI | RGGWCCY | 1 cut(s) | 709 |
| RsaI | GTAC | 3 cut(s) | 283, 383, 659 |
| RsaNI | GTAC | 3 cut(s) | 282, 382, 658 |
| RseI | CAYNNNNRTG | 1 cut(s) | 351 |
| SacI | GAGCTC | 1 cut(s) | 606 |
| SaqAI | TTAA | 3 cut(s) | 99, 393, 701 |
| SatI | GCNGC | 2 cut(s) | 642, 835 |
| Sau3AI | GATC | 4 cut(s) | 183, 303, 490, 811 |
| Sau96I | GGNCC | 1 cut(s) | 709 |
| ScaI | AGTACT | 2 cut(s) | 283, 383 |
| SduI | GDGCHC | 2 cut(s) | 510, 606 |
| SfaNI | GCATC | 1 cut(s) | 536 |
| SinI | GGWCC | 1 cut(s) | 709 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 351 |
| SmlI | CTYRAG | 1 cut(s) | 509 |
| SmoI | CTYRAG | 1 cut(s) | 509 |
| Sse9I | AATT | 3 cut(s) | 455, 636, 754 |
| SsiI | CCGC | 1 cut(s) | 641 |
| SspI | AATATT | 1 cut(s) | 537 |
| SspMI | CTAG | 1 cut(s) | 734 |
| SstI | GAGCTC | 1 cut(s) | 606 |
| TaaI | ACNGT | 3 cut(s) | 57, 289, 565 |
| TaiI | ACGT | 1 cut(s) | 546 |
| TaqI | TCGA | 4 cut(s) | 186, 211, 318, 805 |
| TasI | AATT | 3 cut(s) | 455, 636, 754 |
| TatI | WGTACW | 2 cut(s) | 281, 381 |
| TauI | GCSGC | 1 cut(s) | 644 |
| Tru1I | TTAA | 3 cut(s) | 99, 393, 701 |
| Tru9I | TTAA | 3 cut(s) | 99, 393, 701 |
| TscAI | CASTG | 2 cut(s) | 337, 651 |
| TseI | GCWGC | 1 cut(s) | 834 |
| TspDTI | ATGAA | 1 cut(s) | 90 |
| TspGWI | ACGGA | 1 cut(s) | 807 |
| TspRI | CASTG | 2 cut(s) | 337, 651 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 476 |
| VpaK11BI | GGWCC | 1 cut(s) | 709 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 264 |
| XspI | CTAG | 1 cut(s) | 734 |
| ZraI | GACGTC | 1 cut(s) | 544 |
| ZrmI | AGTACT | 2 cut(s) | 283, 383 |