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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 48 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 50 |
| AccII | CGCG | 1 cut(s) | 215 |
| AciI | CCGC | 2 cut(s) | 215, 437 |
| AclI | AACGTT | 1 cut(s) | 195 |
| AcsI | RAATTY | 2 cut(s) | 64, 419 |
| AcuI | CTGAAG | 2 cut(s) | 238, 353 |
| AfaI | GTAC | 2 cut(s) | 233, 345 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 50, 279, 468, 469 |
| AflII | CTTAAG | 1 cut(s) | 83 |
| AflIII | ACRYGT | 1 cut(s) | 447 |
| AgsI | TTSAA | 3 cut(s) | 59, 200, 241 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 385, 417 |
| AluBI | AGCT | 1 cut(s) | 178 |
| AluI | AGCT | 1 cut(s) | 178 |
| Alw21I | GWGCWC | 1 cut(s) | 180 |
| AoxI | GGCC | 3 cut(s) | 333, 364, 439 |
| ApeKI | GCWGC | 1 cut(s) | 408 |
| ApoI | RAATTY | 2 cut(s) | 64, 419 |
| AspLEI | GCGC | 1 cut(s) | 460 |
| AspS9I | GGNCC | 3 cut(s) | 283, 333, 439 |
| AvaII | GGWCC | 1 cut(s) | 283 |
| BanII | GRGCYC | 1 cut(s) | 180 |
| BbsI | GAAGAC | 1 cut(s) | 309 |
| Bbv12I | GWGCWC | 1 cut(s) | 180 |
| BbvI | GCAGC | 1 cut(s) | 420 |
| BccI | CCATC | 2 cut(s) | 334, 356 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 160, 194 |
| BfaI | CTAG | 1 cut(s) | 347 |
| BfrI | CTTAAG | 1 cut(s) | 83 |
| BfuAI | ACCTGC | 1 cut(s) | 48 |
| BisI | GCNGC | 2 cut(s) | 216, 409 |
| BlsI | GCNGC | 2 cut(s) | 217, 410 |
| Bme18I | GGWCC | 1 cut(s) | 283 |
| BmgT120I | GGNCC | 3 cut(s) | 283, 333, 439 |
| BmiI | GGNNCC | 1 cut(s) | 284 |
| BmrI | ACTGGG | 1 cut(s) | 340 |
| BmsI | GCATC | 1 cut(s) | 110 |
| BmuI | ACTGGG | 1 cut(s) | 340 |
| BpiI | GAAGAC | 1 cut(s) | 309 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 50, 279, 468, 469 |
| Bse1I | ACTGG | 2 cut(s) | 48, 335 |
| Bse3DI | GCAATG | 1 cut(s) | 11 |
| BseGI | GGATG | 2 cut(s) | 367, 388 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 50, 279, 468, 469 |
| BseMI | GCAATG | 1 cut(s) | 11 |
| BseMII | CTCAG | 1 cut(s) | 181 |
| BseNI | ACTGG | 2 cut(s) | 48, 335 |
| BseXI | GCAGC | 1 cut(s) | 420 |
| Bsh1236I | CGCG | 1 cut(s) | 215 |
| BshFI | GGCC | 3 cut(s) | 335, 366, 441 |
| BsiHKAI | GWGCWC | 1 cut(s) | 180 |
| BslFI | GGGAC | 1 cut(s) | 296 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 50, 279, 468, 469 |
| BsmFI | GGGAC | 1 cut(s) | 296 |
| BsmI | GAATGC | 1 cut(s) | 245 |
| BsnI | GGCC | 3 cut(s) | 335, 366, 441 |
| Bsp1286I | GDGCHC | 1 cut(s) | 180 |
| Bsp143I | GATC | 1 cut(s) | 385 |
| BspACI | CCGC | 2 cut(s) | 215, 437 |
| BspANI | GGCC | 3 cut(s) | 335, 366, 441 |
| BspCNI | CTCAG | 1 cut(s) | 180 |
| BspFNI | CGCG | 1 cut(s) | 215 |
| BspLI | GGNNCC | 1 cut(s) | 284 |
| BspMI | ACCTGC | 1 cut(s) | 48 |
| BspTI | CTTAAG | 1 cut(s) | 83 |
| BsrDI | GCAATG | 1 cut(s) | 11 |
| BsrI | ACTGG | 2 cut(s) | 48, 335 |
| BssMI | GATC | 1 cut(s) | 385 |
| Bst4CI | ACNGT | 1 cut(s) | 139 |
| Bst6I | CTCTTC | 1 cut(s) | 242 |
| BstAFI | CTTAAG | 1 cut(s) | 83 |
| BstC8I | GCNNGC | 1 cut(s) | 439 |
| BstDEI | CTNAG | 1 cut(s) | 167 |
| BstF5I | GGATG | 2 cut(s) | 367, 388 |
| BstFNI | CGCG | 1 cut(s) | 215 |
| BstHHI | GCGC | 1 cut(s) | 460 |
| BstKTI | GATC | 1 cut(s) | 388 |
| BstMBI | GATC | 1 cut(s) | 385 |
| BstNSI | RCATGY | 1 cut(s) | 451 |
| BstUI | CGCG | 1 cut(s) | 215 |
| BstV1I | GCAGC | 1 cut(s) | 420 |
| BstV2I | GAAGAC | 1 cut(s) | 309 |
| BsuRI | GGCC | 3 cut(s) | 335, 366, 441 |
| BtsCI | GGATG | 2 cut(s) | 367, 388 |
| BtsI | GCAGTG | 1 cut(s) | 218 |
| BtsIMutI | CAGTG | 2 cut(s) | 55, 218 |
| BveI | ACCTGC | 1 cut(s) | 48 |
| Cac8I | GCNNGC | 1 cut(s) | 439 |
| CfoI | GCGC | 1 cut(s) | 460 |
| Cfr13I | GGNCC | 3 cut(s) | 283, 333, 439 |
| Csp6I | GTAC | 2 cut(s) | 232, 344 |
| CviAII | CATG | 3 cut(s) | 229, 279, 448 |
| CviJI | RGCY | 5 cut(s) | 178, 335, 366, 411, 441 |
| CviKI_1 | RGCY | 5 cut(s) | 178, 335, 366, 411, 441 |
| CviQI | GTAC | 2 cut(s) | 232, 344 |
| DdeI | CTNAG | 1 cut(s) | 167 |
| DpnI | GATC | 1 cut(s) | 387 |
| DpnII | GATC | 1 cut(s) | 385 |
| Eam1104I | CTCTTC | 1 cut(s) | 242 |
| EarI | CTCTTC | 1 cut(s) | 242 |
| Ecl136II | GAGCTC | 1 cut(s) | 178 |
| Eco24I | GRGCYC | 1 cut(s) | 180 |
| Eco47I | GGWCC | 1 cut(s) | 283 |
| Eco53kI | GAGCTC | 1 cut(s) | 178 |
| Eco57I | CTGAAG | 2 cut(s) | 238, 353 |
| EcoICRI | GAGCTC | 1 cut(s) | 178 |
| EcoO109I | RGGNCCY | 1 cut(s) | 283 |
| EcoT22I | ATGCAT | 1 cut(s) | 247 |
| EcoT38I | GRGCYC | 1 cut(s) | 180 |
| FaeI | CATG | 3 cut(s) | 232, 282, 451 |
| FaiI | YATR | 8 cut(s) | 23, 125, 230, 280, 290, 308, 317, 449 |
| FaqI | GGGAC | 1 cut(s) | 296 |
| FatI | CATG | 3 cut(s) | 228, 278, 447 |
| FauI | CCCGC | 1 cut(s) | 430 |
| Fnu4HI | GCNGC | 2 cut(s) | 216, 409 |
| FokI | GGATG | 2 cut(s) | 374, 375 |
| FriOI | GRGCYC | 1 cut(s) | 180 |
| Fsp4HI | GCNGC | 2 cut(s) | 216, 409 |
| FspBI | CTAG | 1 cut(s) | 347 |
| GlaI | GCGC | 1 cut(s) | 459 |
| GluI | GCNGC | 2 cut(s) | 216, 409 |
| HaeIII | GGCC | 3 cut(s) | 335, 366, 441 |
| HhaI | GCGC | 1 cut(s) | 460 |
| Hin1II | CATG | 3 cut(s) | 232, 282, 451 |
| Hin6I | GCGC | 1 cut(s) | 458 |
| HinP1I | GCGC | 1 cut(s) | 458 |
| HinfI | GANTC | 1 cut(s) | 397 |
| Hpy188I | TCNGA | 1 cut(s) | 372 |
| HpyAV | CCTTC | 3 cut(s) | 70, 91, 377 |
| HpyCH4III | ACNGT | 1 cut(s) | 139 |
| HpyCH4IV | ACGT | 1 cut(s) | 195 |
| HpyCH4V | TGCA | 2 cut(s) | 16, 245 |
| HpyF3I | CTNAG | 1 cut(s) | 167 |
| HpySE526I | ACGT | 1 cut(s) | 195 |
| Hsp92II | CATG | 3 cut(s) | 232, 282, 451 |
| HspAI | GCGC | 1 cut(s) | 458 |
| Kzo9I | GATC | 1 cut(s) | 385 |
| LmnI | GCTCC | 1 cut(s) | 175 |
| LpnPI | CCDG | 9 cut(s) | 2, 53, 61, 162, 244, 316, 344, 390, 454 |
| Lsp1109I | GCAGC | 1 cut(s) | 420 |
| LweI | GCATC | 1 cut(s) | 110 |
| MaeI | CTAG | 1 cut(s) | 347 |
| MaeII | ACGT | 1 cut(s) | 195 |
| MalI | GATC | 1 cut(s) | 387 |
| MboI | GATC | 1 cut(s) | 385 |
| MboII | GAAGA | 6 cut(s) | 199, 202, 229, 241, 244, 314 |
| MhlI | GDGCHC | 1 cut(s) | 180 |
| MluCI | AATT | 4 cut(s) | 29, 64, 152, 419 |
| MlyI | GAGTC | 1 cut(s) | 391 |
| MmeI | TCCRAC | 1 cut(s) | 416 |
| MnlI | CCTC | 4 cut(s) | 82, 120, 245, 296 |
| Mph1103I | ATGCAT | 1 cut(s) | 247 |
| MseI | TTAA | 4 cut(s) | 84, 275, 327, 475 |
| MslI | CAYNNNNRTG | 1 cut(s) | 122 |
| MspCI | CTTAAG | 1 cut(s) | 83 |
| Mva1269I | GAATGC | 1 cut(s) | 245 |
| MvnI | CGCG | 1 cut(s) | 215 |
| NdeII | GATC | 1 cut(s) | 385 |
| NlaIII | CATG | 3 cut(s) | 232, 282, 451 |
| NlaIV | GGNNCC | 1 cut(s) | 284 |
| NsiI | ATGCAT | 1 cut(s) | 247 |
| NspI | RCATGY | 1 cut(s) | 451 |
| PciI | ACATGT | 1 cut(s) | 447 |
| PctI | GAATGC | 1 cut(s) | 245 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 50 |
| PkrI | GCNGC | 2 cut(s) | 217, 410 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| PpuMI | RGGWCCY | 1 cut(s) | 283 |
| PscI | ACATGT | 1 cut(s) | 447 |
| Psp124BI | GAGCTC | 1 cut(s) | 180 |
| Psp1406I | AACGTT | 1 cut(s) | 195 |
| Psp5II | RGGWCCY | 1 cut(s) | 283 |
| PspN4I | GGNNCC | 1 cut(s) | 284 |
| PspPI | GGNCC | 3 cut(s) | 283, 333, 439 |
| PspPPI | RGGWCCY | 1 cut(s) | 283 |
| RsaI | GTAC | 2 cut(s) | 233, 345 |
| RsaNI | GTAC | 2 cut(s) | 232, 344 |
| RseI | CAYNNNNRTG | 1 cut(s) | 122 |
| SacI | GAGCTC | 1 cut(s) | 180 |
| SaqAI | TTAA | 4 cut(s) | 84, 275, 327, 475 |
| SatI | GCNGC | 2 cut(s) | 216, 409 |
| Sau3AI | GATC | 1 cut(s) | 385 |
| Sau96I | GGNCC | 3 cut(s) | 283, 333, 439 |
| SchI | GAGTC | 1 cut(s) | 391 |
| SduI | GDGCHC | 1 cut(s) | 180 |
| SetI | ASST | 8 cut(s) | 42, 81, 151, 180, 198, 237, 275, 288 |
| SfaNI | GCATC | 1 cut(s) | 110 |
| SinI | GGWCC | 1 cut(s) | 283 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 122 |
| SmlI | CTYRAG | 1 cut(s) | 83 |
| SmoI | CTYRAG | 1 cut(s) | 83 |
| Sse9I | AATT | 4 cut(s) | 29, 64, 152, 419 |
| SsiI | CCGC | 2 cut(s) | 215, 437 |
| SspMI | CTAG | 1 cut(s) | 347 |
| SstI | GAGCTC | 1 cut(s) | 180 |
| TaaI | ACNGT | 1 cut(s) | 139 |
| TaiI | ACGT | 1 cut(s) | 198 |
| TaqI | TCGA | 2 cut(s) | 180, 266 |
| TasI | AATT | 4 cut(s) | 29, 64, 152, 419 |
| TauI | GCSGC | 1 cut(s) | 218 |
| Tru1I | TTAA | 4 cut(s) | 84, 275, 327, 475 |
| Tru9I | TTAA | 4 cut(s) | 84, 275, 327, 475 |
| TscAI | CASTG | 2 cut(s) | 55, 225 |
| TseI | GCWGC | 1 cut(s) | 408 |
| TspRI | CASTG | 2 cut(s) | 55, 225 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 50 |
| Vha464I | CTTAAG | 1 cut(s) | 83 |
| VpaK11BI | GGWCC | 1 cut(s) | 283 |
| XapI | RAATTY | 2 cut(s) | 64, 419 |
| XceI | RCATGY | 1 cut(s) | 451 |
| XspI | CTAG | 1 cut(s) | 347 |
| Zsp2I | ATGCAT | 1 cut(s) | 247 |