No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 304 |
| AciI | CCGC | 2 cut(s) | 111, 287 |
| AcoI | YGGCCR | 2 cut(s) | 173, 294 |
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AfaI | GTAC | 1 cut(s) | 165 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 79 |
| AgsI | TTSAA | 2 cut(s) | 25, 518 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 135 |
| AjnI | CCWGG | 1 cut(s) | 78 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 89, 121 |
| AluBI | AGCT | 6 cut(s) | 214, 234, 283, 493, 499, 562 |
| AluI | AGCT | 6 cut(s) | 214, 234, 283, 493, 499, 562 |
| AoxI | GGCC | 3 cut(s) | 173, 294, 486 |
| ApeKI | GCWGC | 1 cut(s) | 386 |
| AseI | ATTAAT | 1 cut(s) | 368 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 20 |
| AspS9I | GGNCC | 1 cut(s) | 486 |
| AsuC2I | CCSGG | 1 cut(s) | 39 |
| BaeGI | GKGCMC | 1 cut(s) | 309 |
| BanI | GGYRCC | 1 cut(s) | 304 |
| BbvI | GCAGC | 1 cut(s) | 398 |
| BccI | CCATC | 2 cut(s) | 96, 373 |
| BceAI | ACGGC | 1 cut(s) | 98 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 219, 253 |
| BciT130I | CCWGG | 1 cut(s) | 80 |
| BciVI | GTATCC | 1 cut(s) | 43 |
| BcnI | CCSGG | 1 cut(s) | 39 |
| BfmI | CTRYAG | 1 cut(s) | 238 |
| BfuI | GTATCC | 1 cut(s) | 43 |
| BisI | GCNGC | 1 cut(s) | 387 |
| BlsI | GCNGC | 1 cut(s) | 388 |
| Bme1390I | CCNGG | 2 cut(s) | 39, 80 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 135 |
| BmgT120I | GGNCC | 1 cut(s) | 486 |
| BmiI | GGNNCC | 1 cut(s) | 306 |
| BmrFI | CCNGG | 2 cut(s) | 39, 80 |
| BpmI | CTGGAG | 1 cut(s) | 62 |
| BpuEI | CTTGAG | 1 cut(s) | 287 |
| BpuMI | CCSGG | 1 cut(s) | 39 |
| BsaJI | CCNNGG | 2 cut(s) | 37, 297 |
| BsaWI | WCCGGW | 1 cut(s) | 246 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 79 |
| Bse118I | RCCGGY | 1 cut(s) | 64 |
| BseBI | CCWGG | 1 cut(s) | 80 |
| BseDI | CCNNGG | 2 cut(s) | 37, 297 |
| BseGI | GGATG | 1 cut(s) | 107 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 79 |
| BseMII | CTCAG | 2 cut(s) | 406, 515 |
| BseRI | GAGGAG | 1 cut(s) | 314 |
| BseSI | GKGCMC | 1 cut(s) | 309 |
| BseXI | GCAGC | 1 cut(s) | 398 |
| BshFI | GGCC | 3 cut(s) | 175, 296, 488 |
| BshNI | GGYRCC | 1 cut(s) | 304 |
| BsiSI | CCGG | 3 cut(s) | 39, 65, 247 |
| BslFI | GGGAC | 2 cut(s) | 123, 140 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 79 |
| BsmFI | GGGAC | 2 cut(s) | 123, 140 |
| BsnI | GGCC | 3 cut(s) | 175, 296, 488 |
| Bsp1286I | GDGCHC | 1 cut(s) | 309 |
| Bsp143I | GATC | 2 cut(s) | 184, 270 |
| BspACI | CCGC | 2 cut(s) | 111, 287 |
| BspANI | GGCC | 3 cut(s) | 175, 296, 488 |
| BspCNI | CTCAG | 2 cut(s) | 405, 516 |
| BspLI | GGNNCC | 1 cut(s) | 306 |
| BspMAI | CTGCAG | 1 cut(s) | 242 |
| BspT107I | GGYRCC | 1 cut(s) | 304 |
| BsrFI | RCCGGY | 1 cut(s) | 64 |
| BssAI | RCCGGY | 1 cut(s) | 64 |
| BssECI | CCNNGG | 2 cut(s) | 37, 297 |
| BssMI | GATC | 2 cut(s) | 184, 270 |
| Bst2UI | CCWGG | 1 cut(s) | 80 |
| Bst4CI | ACNGT | 1 cut(s) | 29 |
| BstC8I | GCNNGC | 3 cut(s) | 285, 316, 501 |
| BstDEI | CTNAG | 2 cut(s) | 392, 524 |
| BstF5I | GGATG | 1 cut(s) | 107 |
| BstKTI | GATC | 2 cut(s) | 187, 273 |
| BstMBI | GATC | 2 cut(s) | 184, 270 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 293 |
| BstNI | CCWGG | 1 cut(s) | 80 |
| BstSCI | CCNGG | 2 cut(s) | 37, 78 |
| BstSFI | CTRYAG | 1 cut(s) | 238 |
| BstSLI | GKGCMC | 1 cut(s) | 309 |
| BstV1I | GCAGC | 1 cut(s) | 398 |
| BsuI | GTATCC | 1 cut(s) | 43 |
| BsuRI | GGCC | 3 cut(s) | 175, 296, 488 |
| BtsCI | GGATG | 1 cut(s) | 107 |
| Cac8I | GCNNGC | 3 cut(s) | 285, 316, 501 |
| Cfr10I | RCCGGY | 1 cut(s) | 64 |
| Cfr13I | GGNCC | 1 cut(s) | 486 |
| Csp6I | GTAC | 1 cut(s) | 164 |
| CviQI | GTAC | 1 cut(s) | 164 |
| DdeI | CTNAG | 2 cut(s) | 392, 524 |
| DpnI | GATC | 2 cut(s) | 186, 272 |
| DpnII | GATC | 2 cut(s) | 184, 270 |
| DriI | GACNNNNNGTC | 1 cut(s) | 135 |
| EaeI | YGGCCR | 2 cut(s) | 173, 294 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 135 |
| EciI | GGCGGA | 2 cut(s) | 100, 302 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| EcoRII | CCWGG | 1 cut(s) | 78 |
| FaiI | YATR | 5 cut(s) | 91, 161, 419, 467, 568 |
| FaqI | GGGAC | 2 cut(s) | 123, 140 |
| Fnu4HI | GCNGC | 1 cut(s) | 387 |
| FokI | GGATG | 1 cut(s) | 114 |
| Fsp4HI | GCNGC | 1 cut(s) | 387 |
| GluI | GCNGC | 1 cut(s) | 387 |
| GsuI | CTGGAG | 1 cut(s) | 62 |
| HaeIII | GGCC | 3 cut(s) | 175, 296, 488 |
| HapII | CCGG | 3 cut(s) | 39, 65, 247 |
| HindIII | AAGCTT | 3 cut(s) | 212, 232, 497 |
| HinfI | GANTC | 3 cut(s) | 198, 403, 514 |
| HpaII | CCGG | 3 cut(s) | 39, 65, 247 |
| Hpy166II | GTNNAC | 1 cut(s) | 97 |
| Hpy188I | TCNGA | 5 cut(s) | 15, 129, 219, 395, 525 |
| Hpy8I | GTNNAC | 1 cut(s) | 97 |
| Hpy99I | CGWCG | 1 cut(s) | 259 |
| HpyCH4III | ACNGT | 1 cut(s) | 29 |
| HpyCH4IV | ACGT | 1 cut(s) | 153 |
| HpyCH4V | TGCA | 3 cut(s) | 240, 443, 552 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 293 |
| HpyF3I | CTNAG | 2 cut(s) | 392, 524 |
| HpySE526I | ACGT | 1 cut(s) | 153 |
| Kzo9I | GATC | 2 cut(s) | 184, 270 |
| LpnPI | CCDG | 9 cut(s) | 52, 65, 78, 92, 131, 211, 260, 269, 336 |
| Lsp1109I | GCAGC | 1 cut(s) | 398 |
| MaeII | ACGT | 1 cut(s) | 153 |
| MaeIII | GTNAC | 1 cut(s) | 3 |
| MalI | GATC | 2 cut(s) | 186, 272 |
| MboI | GATC | 2 cut(s) | 184, 270 |
| MboII | GAAGA | 4 cut(s) | 338, 341, 469, 523 |
| MhlI | GDGCHC | 1 cut(s) | 309 |
| MluCI | AATT | 2 cut(s) | 365, 428 |
| MmeI | TCCRAC | 2 cut(s) | 152, 431 |
| MnlI | CCTC | 7 cut(s) | 69, 135, 155, 292, 295, 376, 415 |
| MroXI | GAANNNNTTC | 1 cut(s) | 20 |
| MseI | TTAA | 4 cut(s) | 360, 368, 431, 474 |
| MspI | CCGG | 3 cut(s) | 39, 65, 247 |
| MspR9I | CCNGG | 2 cut(s) | 39, 80 |
| MvaI | CCWGG | 1 cut(s) | 80 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 293 |
| NciI | CCSGG | 1 cut(s) | 39 |
| NdeII | GATC | 2 cut(s) | 184, 270 |
| NlaIV | GGNNCC | 1 cut(s) | 306 |
| NmeAIII | GCCGAG | 1 cut(s) | 322 |
| NmuCI | GTSAC | 1 cut(s) | 3 |
| PdmI | GAANNNNTTC | 1 cut(s) | 20 |
| PfeI | GAWTC | 3 cut(s) | 198, 403, 514 |
| PkrI | GCNGC | 1 cut(s) | 388 |
| PshBI | ATTAAT | 1 cut(s) | 368 |
| Psp6I | CCWGG | 1 cut(s) | 78 |
| PspGI | CCWGG | 1 cut(s) | 78 |
| PspN4I | GGNNCC | 1 cut(s) | 306 |
| PspPI | GGNCC | 1 cut(s) | 486 |
| PstI | CTGCAG | 1 cut(s) | 242 |
| RsaI | GTAC | 1 cut(s) | 165 |
| RsaNI | GTAC | 1 cut(s) | 164 |
| SaqAI | TTAA | 4 cut(s) | 360, 368, 431, 474 |
| SatI | GCNGC | 1 cut(s) | 387 |
| Sau3AI | GATC | 2 cut(s) | 184, 270 |
| Sau96I | GGNCC | 1 cut(s) | 486 |
| ScrFI | CCNGG | 2 cut(s) | 39, 80 |
| SduI | GDGCHC | 1 cut(s) | 309 |
| SfcI | CTRYAG | 1 cut(s) | 238 |
| SmlI | CTYRAG | 1 cut(s) | 266 |
| SmoI | CTYRAG | 1 cut(s) | 266 |
| Sse9I | AATT | 2 cut(s) | 365, 428 |
| SsiI | CCGC | 2 cut(s) | 111, 287 |
| SspI | AATATT | 1 cut(s) | 358 |
| StyD4I | CCNGG | 2 cut(s) | 37, 78 |
| TaaI | ACNGT | 1 cut(s) | 29 |
| TaiI | ACGT | 1 cut(s) | 156 |
| TaqI | TCGA | 1 cut(s) | 201 |
| TasI | AATT | 2 cut(s) | 365, 428 |
| TfiI | GAWTC | 3 cut(s) | 198, 403, 514 |
| Tru1I | TTAA | 4 cut(s) | 360, 368, 431, 474 |
| Tru9I | TTAA | 4 cut(s) | 360, 368, 431, 474 |
| TseFI | GTSAC | 1 cut(s) | 3 |
| TseI | GCWGC | 1 cut(s) | 386 |
| Tsp45I | GTSAC | 1 cut(s) | 3 |
| TspDTI | ATGAA | 2 cut(s) | 348, 545 |
| TspGWI | ACGGA | 1 cut(s) | 114 |
| VspI | ATTAAT | 1 cut(s) | 368 |
| XmnI | GAANNNNTTC | 1 cut(s) | 20 |