Protein ALWAYS EARLY
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 563 |
| AclWI | GGATC | 1 cut(s) | 326 |
| AcuI | CTGAAG | 5 cut(s) | 372, 423, 786, 813, 1230 |
| AcyI | GRCGYC | 1 cut(s) | 1053 |
| AfaI | GTAC | 3 cut(s) | 584, 837, 1438 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 12, 576, 850 |
| AflIII | ACRYGT | 1 cut(s) | 741 |
| AluBI | AGCT | 6 cut(s) | 169, 481, 613, 772, 1027, 1339 |
| AluI | AGCT | 6 cut(s) | 169, 481, 613, 772, 1027, 1339 |
| AlwI | GGATC | 1 cut(s) | 326 |
| Ama87I | CYCGRG | 1 cut(s) | 902 |
| AoxI | GGCC | 4 cut(s) | 3, 749, 864, 885 |
| ApeKI | GCWGC | 3 cut(s) | 261, 911, 1119 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1148, 1180 |
| AspLEI | GCGC | 5 cut(s) | 288, 316, 911, 1146, 1174 |
| AspS9I | GGNCC | 5 cut(s) | 4, 749, 759, 865, 885 |
| AsuC2I | CCSGG | 1 cut(s) | 8 |
| AsuHPI | GGTGA | 1 cut(s) | 833 |
| AvaI | CYCGRG | 1 cut(s) | 902 |
| AvaII | GGWCC | 1 cut(s) | 759 |
| BbsI | GAAGAC | 4 cut(s) | 303, 462, 1161, 1320 |
| BbvI | GCAGC | 3 cut(s) | 248, 898, 1106 |
| BccI | CCATC | 2 cut(s) | 251, 1109 |
| BceAI | ACGGC | 1 cut(s) | 230 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 893, 927 |
| BcnI | CCSGG | 1 cut(s) | 8 |
| BfaI | CTAG | 2 cut(s) | 224, 1082 |
| BfoI | RGCGCY | 1 cut(s) | 912 |
| BglII | AGATCT | 1 cut(s) | 1189 |
| BisI | GCNGC | 3 cut(s) | 262, 912, 1120 |
| BlsI | GCNGC | 3 cut(s) | 263, 913, 1121 |
| Bme1390I | CCNGG | 1 cut(s) | 8 |
| Bme18I | GGWCC | 1 cut(s) | 759 |
| BmeT110I | CYCGRG | 1 cut(s) | 902 |
| BmgT120I | GGNCC | 5 cut(s) | 4, 749, 759, 865, 885 |
| BmiI | GGNNCC | 3 cut(s) | 6, 148, 1006 |
| BmrFI | CCNGG | 1 cut(s) | 8 |
| BmsI | GCATC | 6 cut(s) | 260, 301, 336, 1118, 1159, 1194 |
| BpiI | GAAGAC | 4 cut(s) | 303, 462, 1161, 1320 |
| BpuEI | CTTGAG | 2 cut(s) | 361, 1219 |
| BpuMI | CCSGG | 1 cut(s) | 8 |
| BsaHI | GRCGYC | 1 cut(s) | 1053 |
| BsaJI | CCNNGG | 2 cut(s) | 6, 903 |
| BsaWI | WCCGGW | 1 cut(s) | 630 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 12, 576, 850 |
| Bse1I | ACTGG | 6 cut(s) | 523, 548, 564, 818, 1381, 1406 |
| Bse3DI | GCAATG | 3 cut(s) | 405, 942, 1263 |
| BseDI | CCNNGG | 2 cut(s) | 6, 903 |
| BseGI | GGATG | 3 cut(s) | 351, 822, 1209 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 12, 576, 850 |
| BseMI | GCAATG | 3 cut(s) | 405, 942, 1263 |
| BseMII | CTCAG | 4 cut(s) | 248, 491, 1106, 1349 |
| BseNI | ACTGG | 6 cut(s) | 523, 548, 564, 818, 1381, 1406 |
| BseXI | GCAGC | 3 cut(s) | 248, 898, 1106 |
| BseYI | CCCAGC | 2 cut(s) | 481, 1339 |
| BsgI | GTGCAG | 2 cut(s) | 363, 1221 |
| BshFI | GGCC | 4 cut(s) | 5, 751, 866, 887 |
| BsiHKCI | CYCGRG | 1 cut(s) | 902 |
| BsiSI | CCGG | 2 cut(s) | 8, 631 |
| BslFI | GGGAC | 2 cut(s) | 223, 1081 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 12, 576, 850 |
| BsmFI | GGGAC | 2 cut(s) | 223, 1081 |
| BsnI | GGCC | 4 cut(s) | 5, 751, 866, 887 |
| BsoBI | CYCGRG | 1 cut(s) | 902 |
| Bsp143I | GATC | 3 cut(s) | 331, 845, 1189 |
| BspACI | CCGC | 1 cut(s) | 563 |
| BspANI | GGCC | 4 cut(s) | 5, 751, 866, 887 |
| BspCNI | CTCAG | 4 cut(s) | 247, 490, 1105, 1348 |
| BspLI | GGNNCC | 3 cut(s) | 6, 148, 1006 |
| BspPI | GGATC | 1 cut(s) | 326 |
| BspQI | GCTCTTC | 1 cut(s) | 762 |
| BsrDI | GCAATG | 3 cut(s) | 405, 942, 1263 |
| BsrI | ACTGG | 6 cut(s) | 523, 548, 564, 818, 1381, 1406 |
| BssECI | CCNNGG | 2 cut(s) | 6, 903 |
| BssMI | GATC | 3 cut(s) | 331, 845, 1189 |
| BssNI | GRCGYC | 1 cut(s) | 1053 |
| Bst4CI | ACNGT | 5 cut(s) | 119, 273, 510, 977, 1368 |
| Bst6I | CTCTTC | 4 cut(s) | 307, 762, 903, 1165 |
| BstACI | GRCGYC | 1 cut(s) | 1053 |
| BstC8I | GCNNGC | 2 cut(s) | 171, 1029 |
| BstDEI | CTNAG | 6 cut(s) | 234, 411, 477, 1092, 1269, 1335 |
| BstF5I | GGATG | 3 cut(s) | 351, 822, 1209 |
| BstH2I | RGCGCY | 1 cut(s) | 912 |
| BstHHI | GCGC | 5 cut(s) | 288, 316, 911, 1146, 1174 |
| BstKTI | GATC | 3 cut(s) | 334, 848, 1192 |
| BstMBI | GATC | 3 cut(s) | 331, 845, 1189 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 166, 1024 |
| BstNSI | RCATGY | 1 cut(s) | 745 |
| BstSCI | CCNGG | 1 cut(s) | 6 |
| BstV1I | GCAGC | 3 cut(s) | 248, 898, 1106 |
| BstV2I | GAAGAC | 4 cut(s) | 303, 462, 1161, 1320 |
| BstX2I | RGATCY | 2 cut(s) | 331, 1189 |
| BstYI | RGATCY | 2 cut(s) | 331, 1189 |
| BsuRI | GGCC | 4 cut(s) | 5, 751, 866, 887 |
| BtsCI | GGATG | 3 cut(s) | 351, 822, 1209 |
| BtsI | GCAGTG | 3 cut(s) | 459, 1127, 1317 |
| Cac8I | GCNNGC | 2 cut(s) | 171, 1029 |
| CfoI | GCGC | 5 cut(s) | 288, 316, 911, 1146, 1174 |
| Cfr13I | GGNCC | 5 cut(s) | 4, 749, 759, 865, 885 |
| CseI | GACGC | 1 cut(s) | 1061 |
| Csp6I | GTAC | 3 cut(s) | 583, 836, 1437 |
| CviAII | CATG | 1 cut(s) | 742 |
| CviQI | GTAC | 3 cut(s) | 583, 836, 1437 |
| DdeI | CTNAG | 6 cut(s) | 234, 411, 477, 1092, 1269, 1335 |
| DpnI | GATC | 3 cut(s) | 333, 847, 1191 |
| DpnII | GATC | 3 cut(s) | 331, 845, 1189 |
| Eam1104I | CTCTTC | 4 cut(s) | 307, 762, 903, 1165 |
| EarI | CTCTTC | 4 cut(s) | 307, 762, 903, 1165 |
| EciI | GGCGGA | 1 cut(s) | 578 |
| Eco32I | GATATC | 1 cut(s) | 794 |
| Eco47I | GGWCC | 1 cut(s) | 759 |
| Eco57I | CTGAAG | 5 cut(s) | 372, 423, 786, 813, 1230 |
| Eco88I | CYCGRG | 1 cut(s) | 902 |
| EcoO109I | RGGNCCY | 3 cut(s) | 749, 865, 885 |
| EcoRV | GATATC | 1 cut(s) | 794 |
| FaeI | CATG | 1 cut(s) | 745 |
| FaqI | GGGAC | 2 cut(s) | 223, 1081 |
| FatI | CATG | 1 cut(s) | 741 |
| Fnu4HI | GCNGC | 3 cut(s) | 262, 912, 1120 |
| FokI | GGATG | 3 cut(s) | 358, 829, 1216 |
| Fsp4HI | GCNGC | 3 cut(s) | 262, 912, 1120 |
| FspBI | CTAG | 2 cut(s) | 224, 1082 |
| GlaI | GCGC | 5 cut(s) | 287, 315, 910, 1145, 1173 |
| GluI | GCNGC | 3 cut(s) | 262, 912, 1120 |
| GsaI | CCCAGC | 2 cut(s) | 485, 1343 |
| HaeII | RGCGCY | 1 cut(s) | 912 |
| HaeIII | GGCC | 4 cut(s) | 5, 751, 866, 887 |
| HapII | CCGG | 2 cut(s) | 8, 631 |
| HgaI | GACGC | 1 cut(s) | 1061 |
| HhaI | GCGC | 5 cut(s) | 288, 316, 911, 1146, 1174 |
| Hin1I | GRCGYC | 1 cut(s) | 1053 |
| Hin1II | CATG | 1 cut(s) | 745 |
| Hin6I | GCGC | 5 cut(s) | 286, 314, 909, 1144, 1172 |
| HinP1I | GCGC | 5 cut(s) | 286, 314, 909, 1144, 1172 |
| HindIII | AAGCTT | 2 cut(s) | 167, 1025 |
| HinfI | GANTC | 6 cut(s) | 299, 371, 433, 1157, 1229, 1291 |
| HpaII | CCGG | 2 cut(s) | 8, 631 |
| HphI | GGTGA | 1 cut(s) | 833 |
| Hpy166II | GTNNAC | 2 cut(s) | 122, 980 |
| Hpy188I | TCNGA | 6 cut(s) | 256, 310, 514, 739, 1114, 1372 |
| Hpy188III | TCNNGA | 4 cut(s) | 224, 625, 902, 1082 |
| Hpy8I | GTNNAC | 2 cut(s) | 122, 980 |
| HpyAV | CCTTC | 6 cut(s) | 447, 570, 631, 782, 814, 1064 |
| HpyCH4III | ACNGT | 5 cut(s) | 119, 273, 510, 977, 1368 |
| HpyCH4IV | ACGT | 1 cut(s) | 838 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 166, 1024 |
| HpyF3I | CTNAG | 6 cut(s) | 234, 411, 477, 1092, 1269, 1335 |
| HpySE526I | ACGT | 1 cut(s) | 838 |
| Hsp92I | GRCGYC | 1 cut(s) | 1053 |
| Hsp92II | CATG | 1 cut(s) | 745 |
| HspAI | GCGC | 5 cut(s) | 286, 314, 909, 1144, 1172 |
| Kzo9I | GATC | 3 cut(s) | 331, 845, 1189 |
| LguI | GCTCTTC | 1 cut(s) | 762 |
| Lsp1109I | GCAGC | 3 cut(s) | 248, 898, 1106 |
| LweI | GCATC | 6 cut(s) | 260, 301, 336, 1118, 1159, 1194 |
| MaeI | CTAG | 2 cut(s) | 224, 1082 |
| MaeII | ACGT | 1 cut(s) | 838 |
| MalI | GATC | 3 cut(s) | 333, 847, 1191 |
| MboI | GATC | 3 cut(s) | 331, 845, 1189 |
| MfeI | CAATTG | 2 cut(s) | 246, 1104 |
| MflI | RGATCY | 2 cut(s) | 331, 1189 |
| MluCI | AATT | 5 cut(s) | 182, 246, 1040, 1104, 1441 |
| MlyI | GAGTC | 2 cut(s) | 293, 1151 |
| MseI | TTAA | 2 cut(s) | 552, 1410 |
| MslI | CAYNNNNRTG | 1 cut(s) | 783 |
| MspA1I | CMGCKG | 2 cut(s) | 481, 1339 |
| MspI | CCGG | 2 cut(s) | 8, 631 |
| MspR9I | CCNGG | 1 cut(s) | 8 |
| MunI | CAATTG | 2 cut(s) | 246, 1104 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 166, 1024 |
| NciI | CCSGG | 1 cut(s) | 8 |
| NdeII | GATC | 3 cut(s) | 331, 845, 1189 |
| NlaIII | CATG | 1 cut(s) | 745 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 148, 1006 |
| NmuCI | GTSAC | 8 cut(s) | 113, 267, 599, 839, 944, 971, 1125, 1417 |
| NspI | RCATGY | 1 cut(s) | 745 |
| PciI | ACATGT | 1 cut(s) | 741 |
| PciSI | GCTCTTC | 1 cut(s) | 762 |
| PfeI | GAWTC | 4 cut(s) | 371, 433, 1229, 1291 |
| PkrI | GCNGC | 3 cut(s) | 263, 913, 1121 |
| PleI | GAGTC | 2 cut(s) | 293, 1151 |
| PpsI | GAGTC | 2 cut(s) | 293, 1151 |
| PscI | ACATGT | 1 cut(s) | 741 |
| PspFI | CCCAGC | 2 cut(s) | 481, 1339 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 148, 1006 |
| PspPI | GGNCC | 5 cut(s) | 4, 749, 759, 865, 885 |
| PsuI | RGATCY | 2 cut(s) | 331, 1189 |
| PvuII | CAGCTG | 2 cut(s) | 481, 1339 |
| RsaI | GTAC | 3 cut(s) | 584, 837, 1438 |
| RsaNI | GTAC | 3 cut(s) | 583, 836, 1437 |
| RseI | CAYNNNNRTG | 1 cut(s) | 783 |
| SapI | GCTCTTC | 1 cut(s) | 762 |
| SaqAI | TTAA | 2 cut(s) | 552, 1410 |
| SatI | GCNGC | 3 cut(s) | 262, 912, 1120 |
| Sau3AI | GATC | 3 cut(s) | 331, 845, 1189 |
| Sau96I | GGNCC | 5 cut(s) | 4, 749, 759, 865, 885 |
| SchI | GAGTC | 2 cut(s) | 293, 1151 |
| ScrFI | CCNGG | 1 cut(s) | 8 |
| SfaNI | GCATC | 6 cut(s) | 260, 301, 336, 1118, 1159, 1194 |
| SinI | GGWCC | 1 cut(s) | 759 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 783 |
| SmlI | CTYRAG | 2 cut(s) | 340, 1198 |
| SmoI | CTYRAG | 2 cut(s) | 340, 1198 |
| Sse9I | AATT | 5 cut(s) | 182, 246, 1040, 1104, 1441 |
| SsiI | CCGC | 1 cut(s) | 563 |
| SspMI | CTAG | 2 cut(s) | 224, 1082 |
| StyD4I | CCNGG | 1 cut(s) | 6 |
| TaaI | ACNGT | 5 cut(s) | 119, 273, 510, 977, 1368 |
| TaiI | ACGT | 1 cut(s) | 841 |
| TaqI | TCGA | 3 cut(s) | 387, 624, 1245 |
| TasI | AATT | 5 cut(s) | 182, 246, 1040, 1104, 1441 |
| TatI | WGTACW | 1 cut(s) | 582 |
| TfiI | GAWTC | 4 cut(s) | 371, 433, 1229, 1291 |
| Tru1I | TTAA | 2 cut(s) | 552, 1410 |
| Tru9I | TTAA | 2 cut(s) | 552, 1410 |
| TseFI | GTSAC | 8 cut(s) | 113, 267, 599, 839, 944, 971, 1125, 1417 |
| TseI | GCWGC | 3 cut(s) | 261, 911, 1119 |
| Tsp45I | GTSAC | 8 cut(s) | 113, 267, 599, 839, 944, 971, 1125, 1417 |
| TspDTI | ATGAA | 5 cut(s) | 309, 801, 833, 893, 1167 |
| TspGWI | ACGGA | 4 cut(s) | 202, 324, 1060, 1424 |
| VpaK11BI | GGWCC | 1 cut(s) | 759 |
| XbaI | TCTAGA | 2 cut(s) | 223, 1081 |
| XceI | RCATGY | 1 cut(s) | 745 |
| XspI | CTAG | 2 cut(s) | 224, 1082 |