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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 249 |
| AccB1I | GGYRCC | 1 cut(s) | 85 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 466 |
| AccI | GTMKAC | 1 cut(s) | 517 |
| AciI | CCGC | 1 cut(s) | 104 |
| AfaI | GTAC | 1 cut(s) | 350 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 42, 291, 340, 466, 478 |
| AgsI | TTSAA | 2 cut(s) | 25, 122 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 475 |
| AjnI | CCWGG | 3 cut(s) | 80, 90, 435 |
| AluBI | AGCT | 6 cut(s) | 42, 71, 211, 220, 274, 400 |
| AluI | AGCT | 6 cut(s) | 42, 71, 211, 220, 274, 400 |
| Alw26I | GTCTC | 2 cut(s) | 173, 385 |
| Ama87I | CYCGRG | 1 cut(s) | 37 |
| AoxI | GGCC | 2 cut(s) | 267, 429 |
| ApeKI | GCWGC | 2 cut(s) | 167, 499 |
| AspLEI | GCGC | 1 cut(s) | 250 |
| AspS9I | GGNCC | 3 cut(s) | 4, 267, 469 |
| AsuHPI | GGTGA | 1 cut(s) | 188 |
| AvaI | CYCGRG | 1 cut(s) | 37 |
| AvaII | GGWCC | 2 cut(s) | 4, 469 |
| BaeGI | GKGCMC | 1 cut(s) | 90 |
| BanI | GGYRCC | 1 cut(s) | 85 |
| BbvI | GCAGC | 2 cut(s) | 179, 511 |
| BccI | CCATC | 1 cut(s) | 154 |
| BciT130I | CCWGG | 3 cut(s) | 82, 92, 437 |
| BcoDI | GTCTC | 2 cut(s) | 173, 385 |
| BfaI | CTAG | 1 cut(s) | 212 |
| BglII | AGATCT | 1 cut(s) | 259 |
| BisI | GCNGC | 2 cut(s) | 168, 500 |
| BlsI | GCNGC | 2 cut(s) | 169, 501 |
| Bme1390I | CCNGG | 3 cut(s) | 82, 92, 437 |
| Bme18I | GGWCC | 2 cut(s) | 4, 469 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 475 |
| BmeT110I | CYCGRG | 1 cut(s) | 37 |
| BmgT120I | GGNCC | 3 cut(s) | 4, 267, 469 |
| BmiI | GGNNCC | 2 cut(s) | 87, 240 |
| BmrFI | CCNGG | 3 cut(s) | 82, 92, 437 |
| BoxI | GACNNNNGTC | 1 cut(s) | 9 |
| BpuEI | CTTGAG | 1 cut(s) | 422 |
| BsaI | GGTCTC | 1 cut(s) | 385 |
| BsaJI | CCNNGG | 4 cut(s) | 7, 81, 90, 435 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 38, 68 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 42, 291, 340, 466, 478 |
| Bse1I | ACTGG | 2 cut(s) | 32, 503 |
| BseBI | CCWGG | 3 cut(s) | 82, 92, 437 |
| BseDI | CCNNGG | 4 cut(s) | 7, 81, 90, 435 |
| BseGI | GGATG | 2 cut(s) | 445, 457 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 42, 291, 340, 466, 478 |
| BseNI | ACTGG | 2 cut(s) | 32, 503 |
| BseSI | GKGCMC | 1 cut(s) | 90 |
| BseXI | GCAGC | 2 cut(s) | 179, 511 |
| BseYI | CCCAGC | 1 cut(s) | 42 |
| BsgI | GTGCAG | 1 cut(s) | 76 |
| BshFI | GGCC | 2 cut(s) | 269, 431 |
| BshNI | GGYRCC | 1 cut(s) | 85 |
| BsiHKCI | CYCGRG | 1 cut(s) | 37 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 42, 291, 340, 466, 478 |
| BsmAI | GTCTC | 2 cut(s) | 173, 385 |
| BsmI | GAATGC | 1 cut(s) | 250 |
| BsnI | GGCC | 2 cut(s) | 269, 431 |
| Bso31I | GGTCTC | 1 cut(s) | 385 |
| BsoBI | CYCGRG | 1 cut(s) | 37 |
| Bsp1286I | GDGCHC | 1 cut(s) | 90 |
| Bsp1407I | TGTACA | 1 cut(s) | 348 |
| Bsp143I | GATC | 1 cut(s) | 259 |
| BspACI | CCGC | 1 cut(s) | 104 |
| BspANI | GGCC | 2 cut(s) | 269, 431 |
| BspLI | GGNNCC | 2 cut(s) | 87, 240 |
| BspT107I | GGYRCC | 1 cut(s) | 85 |
| BspTNI | GGTCTC | 1 cut(s) | 385 |
| BsrGI | TGTACA | 1 cut(s) | 348 |
| BsrI | ACTGG | 2 cut(s) | 32, 503 |
| BssECI | CCNNGG | 4 cut(s) | 7, 81, 90, 435 |
| BssMI | GATC | 1 cut(s) | 259 |
| Bst2UI | CCWGG | 3 cut(s) | 82, 92, 437 |
| Bst4CI | ACNGT | 1 cut(s) | 8 |
| Bst6I | CTCTTC | 1 cut(s) | 102 |
| BstAUI | TGTACA | 1 cut(s) | 348 |
| BstC8I | GCNNGC | 2 cut(s) | 97, 222 |
| BstDSI | CCRYGG | 1 cut(s) | 7 |
| BstF5I | GGATG | 2 cut(s) | 445, 457 |
| BstHHI | GCGC | 1 cut(s) | 250 |
| BstKTI | GATC | 1 cut(s) | 262 |
| BstMAI | GTCTC | 2 cut(s) | 173, 385 |
| BstMBI | GATC | 1 cut(s) | 259 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 101, 217 |
| BstNI | CCWGG | 3 cut(s) | 82, 92, 437 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 9 |
| BstSCI | CCNGG | 3 cut(s) | 80, 90, 435 |
| BstSLI | GKGCMC | 1 cut(s) | 90 |
| BstV1I | GCAGC | 2 cut(s) | 179, 511 |
| BstX2I | RGATCY | 1 cut(s) | 259 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 199 |
| BstYI | RGATCY | 1 cut(s) | 259 |
| BsuRI | GGCC | 2 cut(s) | 269, 431 |
| BtgI | CCRYGG | 1 cut(s) | 7 |
| BtsCI | GGATG | 2 cut(s) | 445, 457 |
| BtsIMutI | CAGTG | 1 cut(s) | 510 |
| Cac8I | GCNNGC | 2 cut(s) | 97, 222 |
| CfoI | GCGC | 1 cut(s) | 250 |
| Cfr13I | GGNCC | 3 cut(s) | 4, 267, 469 |
| CseI | GACGC | 1 cut(s) | 465 |
| Csp6I | GTAC | 1 cut(s) | 349 |
| CviAII | CATG | 2 cut(s) | 418, 525 |
| CviQI | GTAC | 1 cut(s) | 349 |
| DpnI | GATC | 1 cut(s) | 261 |
| DpnII | GATC | 1 cut(s) | 259 |
| DriI | GACNNNNNGTC | 1 cut(s) | 475 |
| Eam1104I | CTCTTC | 1 cut(s) | 102 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 475 |
| EarI | CTCTTC | 1 cut(s) | 102 |
| Eco31I | GGTCTC | 1 cut(s) | 385 |
| Eco47I | GGWCC | 2 cut(s) | 4, 469 |
| Eco88I | CYCGRG | 1 cut(s) | 37 |
| EcoRII | CCWGG | 3 cut(s) | 80, 90, 435 |
| FaeI | CATG | 2 cut(s) | 421, 528 |
| FaiI | YATR | 5 cut(s) | 74, 265, 419, 497, 526 |
| FatI | CATG | 2 cut(s) | 417, 524 |
| FblI | GTMKAC | 1 cut(s) | 517 |
| Fnu4HI | GCNGC | 2 cut(s) | 168, 500 |
| FokI | GGATG | 2 cut(s) | 452, 464 |
| Fsp4HI | GCNGC | 2 cut(s) | 168, 500 |
| FspBI | CTAG | 1 cut(s) | 212 |
| FspI | TGCGCA | 1 cut(s) | 249 |
| GlaI | GCGC | 1 cut(s) | 249 |
| GluI | GCNGC | 2 cut(s) | 168, 500 |
| GsaI | CCCAGC | 1 cut(s) | 46 |
| HaeIII | GGCC | 2 cut(s) | 269, 431 |
| HgaI | GACGC | 1 cut(s) | 465 |
| HhaI | GCGC | 1 cut(s) | 250 |
| Hin1II | CATG | 2 cut(s) | 421, 528 |
| Hin6I | GCGC | 1 cut(s) | 248 |
| HinP1I | GCGC | 1 cut(s) | 248 |
| HincII | GTYRAC | 1 cut(s) | 518 |
| HindII | GTYRAC | 1 cut(s) | 518 |
| HindIII | AAGCTT | 1 cut(s) | 218 |
| HphI | GGTGA | 1 cut(s) | 188 |
| Hpy166II | GTNNAC | 1 cut(s) | 518 |
| Hpy188I | TCNGA | 1 cut(s) | 481 |
| Hpy8I | GTNNAC | 1 cut(s) | 518 |
| Hpy99I | CGWCG | 2 cut(s) | 481, 522 |
| HpyAV | CCTTC | 1 cut(s) | 313 |
| HpyCH4III | ACNGT | 1 cut(s) | 8 |
| HpyCH4V | TGCA | 2 cut(s) | 57, 499 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 101, 217 |
| Hsp92II | CATG | 2 cut(s) | 421, 528 |
| HspAI | GCGC | 1 cut(s) | 248 |
| Kzo9I | GATC | 1 cut(s) | 259 |
| Lsp1109I | GCAGC | 2 cut(s) | 179, 511 |
| MaeI | CTAG | 1 cut(s) | 212 |
| MalI | GATC | 1 cut(s) | 261 |
| MboI | GATC | 1 cut(s) | 259 |
| MboII | GAAGA | 1 cut(s) | 119 |
| MflI | RGATCY | 1 cut(s) | 259 |
| MhlI | GDGCHC | 1 cut(s) | 90 |
| MluCI | AATT | 2 cut(s) | 20, 117 |
| MmeI | TCCRAC | 1 cut(s) | 459 |
| MseI | TTAA | 1 cut(s) | 297 |
| MspR9I | CCNGG | 3 cut(s) | 82, 92, 437 |
| Mva1269I | GAATGC | 1 cut(s) | 250 |
| MvaI | CCWGG | 3 cut(s) | 82, 92, 437 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 101, 217 |
| NdeII | GATC | 1 cut(s) | 259 |
| NlaIII | CATG | 2 cut(s) | 421, 528 |
| NlaIV | GGNNCC | 2 cut(s) | 87, 240 |
| NsbI | TGCGCA | 1 cut(s) | 249 |
| PaeR7I | CTCGAG | 1 cut(s) | 37 |
| PctI | GAATGC | 1 cut(s) | 250 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 466 |
| PkrI | GCNGC | 2 cut(s) | 169, 501 |
| PshAI | GACNNNNGTC | 1 cut(s) | 9 |
| Psp6I | CCWGG | 3 cut(s) | 80, 90, 435 |
| PspFI | CCCAGC | 1 cut(s) | 42 |
| PspGI | CCWGG | 3 cut(s) | 80, 90, 435 |
| PspN4I | GGNNCC | 2 cut(s) | 87, 240 |
| PspPI | GGNCC | 3 cut(s) | 4, 267, 469 |
| PspXI | VCTCGAGB | 1 cut(s) | 37 |
| PsuI | RGATCY | 1 cut(s) | 259 |
| RsaI | GTAC | 1 cut(s) | 350 |
| RsaNI | GTAC | 1 cut(s) | 349 |
| SalI | GTCGAC | 1 cut(s) | 516 |
| SaqAI | TTAA | 1 cut(s) | 297 |
| SatI | GCNGC | 2 cut(s) | 168, 500 |
| Sau3AI | GATC | 1 cut(s) | 259 |
| Sau96I | GGNCC | 3 cut(s) | 4, 267, 469 |
| ScrFI | CCNGG | 3 cut(s) | 82, 92, 437 |
| SduI | GDGCHC | 1 cut(s) | 90 |
| Sfr274I | CTCGAG | 1 cut(s) | 37 |
| SinI | GGWCC | 2 cut(s) | 4, 469 |
| SlaI | CTCGAG | 1 cut(s) | 37 |
| SmlI | CTYRAG | 2 cut(s) | 37, 401 |
| SmoI | CTYRAG | 2 cut(s) | 37, 401 |
| Sse9I | AATT | 2 cut(s) | 20, 117 |
| SsiI | CCGC | 1 cut(s) | 104 |
| SspMI | CTAG | 1 cut(s) | 212 |
| StyD4I | CCNGG | 3 cut(s) | 80, 90, 435 |
| TaaI | ACNGT | 1 cut(s) | 8 |
| TaqI | TCGA | 2 cut(s) | 38, 517 |
| TasI | AATT | 2 cut(s) | 20, 117 |
| TatI | WGTACW | 1 cut(s) | 348 |
| Tru1I | TTAA | 1 cut(s) | 297 |
| Tru9I | TTAA | 1 cut(s) | 297 |
| TscAI | CASTG | 1 cut(s) | 510 |
| TseI | GCWGC | 2 cut(s) | 167, 499 |
| TspDTI | ATGAA | 4 cut(s) | 327, 406, 456, 468 |
| TspRI | CASTG | 1 cut(s) | 510 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 466 |
| VpaK11BI | GGWCC | 2 cut(s) | 4, 469 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 196 |
| XhoI | CTCGAG | 1 cut(s) | 37 |
| XmiI | GTMKAC | 1 cut(s) | 517 |
| XspI | CTAG | 1 cut(s) | 212 |