FvH4_4g00252

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000607
Physical Location & Seq
Reverse (-)
270478 .. 271999
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00252.t1

Sequence Viewer

Length: 888 bp
ATGACGACGGCTTTTTGGTGGCTCAGCGGCTTCGGAGGGGATGAGGATGGGTACTCCGAGGTTAAGCATGTTGATGCTAGAGCAATCCCAGGATGGGTGACCTACTGGGAAGCTGCTCCTGAGCTGCCGGAAACAAAACTGCCGGGCCTTGTTGTACACGGGCCGGTCCCGGACCCTCAAATTCAGAAAAACTTTACGGCCCAAAAGCCCGACTTAGTGACTTACACATTCTACGCTGCCGCCGCCCGTCGACTCGCCACTCTAAGACGCATCGACGCCTCACCAGTCGCCATCATCTGCCTCCGACCACAAACCAAGACACCAAGTCGCCCACTGCCTCCAGTACAAATTCTAATCTCTCATTCCCTCTCAACTTCCATCGCCTTTGCAGGTTTGTTTGAATTCTGGATTTTTGATTTTACGAAGATGGGTTGTAAAATCATCTGGGTTTTTGGAGCAGGAGGGGTAGTTGAAGGTGCGGGTCCAGTCGGCGATGTGACCGAGCTTCTCGGAGCGGGAGAAAGGGGCGAAGGGGATGTTGAGGGGGAGGTTGGAGGTGGTAGTTGTGGTGATGGCGCAGTCCGGTGGGCCCCAGCCGTCGGGGTTGAAGCCTTGAAGGGGACGCCGCTGACTTCGAAGGAAGGAGAGGGAGGTGAGGGAGGAGAGGGAGGAGAATCAGACTTGGAGATGGCAGGCAGAACGTTGTTGTCCAGTTTATGTCGGAAAAAGACCGAAAACCGGCAAGACCGGCCCGATTTATGCCGGTTCGGGCTTTTGCCGGTCCCGGAAATTTTTAAGGCTAAGACCGGCCCGTCTGCACACCTGTCGGTCCGGGCTCGGGCTTCACTTTTGGGGTGTCGGTCTGAGACCGTGCCCAGCCCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

31.36

Weight (kDa)

5.54

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 811
Acc36I ACCTGC 1 cut(s) 380
AccBSI CCGCTC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 250
AciI CCGC 6 cut(s) 27, 240, 243, 479, 515, 626
AclI AACGTT 1 cut(s) 701
AcsI RAATTY 4 cut(s) 180, 348, 401, 789
AcyI GRCGYC 2 cut(s) 276, 623
AfaI GTAC 3 cut(s) 53, 156, 345
AfiI CCNNNNNNNGG 5 cut(s) 94, 599, 618, 738, 838
AgsI TTSAA 4 cut(s) 401, 473, 608, 616
AhdI GACNNNNNGTC 1 cut(s) 324
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 3 cut(s) 113, 124, 505
AluI AGCT 3 cut(s) 113, 124, 505
Alw26I GTCTC 1 cut(s) 860
Ama87I CYCGRG 1 cut(s) 837
AoxI GGCC 6 cut(s) 145, 161, 198, 588, 749, 808
ApaI GGGCCC 1 cut(s) 592
ApeKI GCWGC 3 cut(s) 113, 124, 236
ApoI RAATTY 4 cut(s) 180, 348, 401, 789
AspLEI GCGC 1 cut(s) 578
AsuC2I CCSGG 4 cut(s) 144, 170, 785, 833
AsuHPI GGTGA 4 cut(s) 109, 273, 581, 665
AsuII TTCGAA 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 837
AvaII GGWCC 5 cut(s) 166, 172, 482, 781, 829
BaeGI GKGCMC 2 cut(s) 592, 876
BanII GRGCYC 2 cut(s) 592, 838
BbvI GCAGC 3 cut(s) 100, 111, 223
BccI CCATC 7 cut(s) 41, 87, 299, 386, 421, 566, 682
BceAI ACGGC 3 cut(s) 24, 213, 581
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BciT130I CCWGG 1 cut(s) 90
BcnI CCSGG 4 cut(s) 144, 170, 785, 833
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 7 cut(s) 28, 114, 125, 237, 240, 243, 626
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 7 cut(s) 29, 115, 126, 238, 241, 244, 627
Bme1390I CCNGG 5 cut(s) 90, 144, 170, 785, 833
Bme18I GGWCC 5 cut(s) 166, 172, 482, 781, 829
BmeRI GACNNNNNGTC 1 cut(s) 324
BmeT110I CYCGRG 1 cut(s) 837
BmiI GGNNCC 6 cut(s) 168, 174, 483, 590, 591, 783
BmrFI CCNGG 5 cut(s) 90, 144, 170, 785, 833
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 2 cut(s) 64, 279
BmuI ACTGGG 1 cut(s) 115
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 635
BpuMI CCSGG 4 cut(s) 144, 170, 785, 833
BsaHI GRCGYC 2 cut(s) 276, 623
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 2 cut(s) 57, 88
BsaWI WCCGGW 1 cut(s) 582
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 599, 618, 738, 838
Bse118I RCCGGY 6 cut(s) 163, 738, 747, 762, 778, 806
Bse1I ACTGG 5 cut(s) 110, 284, 341, 485, 711
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 57, 88
BseGI GGATG 4 cut(s) 46, 52, 98, 541
BseLI CCNNNNNNNGG 5 cut(s) 94, 599, 618, 738, 838
BseMII CTCAG 3 cut(s) 37, 111, 855
BseNI ACTGG 5 cut(s) 110, 284, 341, 485, 711
BseRI GAGGAG 2 cut(s) 675, 684
BseSI GKGCMC 2 cut(s) 592, 876
BseXI GCAGC 3 cut(s) 100, 111, 223
BseYI CCCAGC 2 cut(s) 592, 875
BsgI GTGCAG 1 cut(s) 801
BshFI GGCC 6 cut(s) 147, 163, 200, 590, 751, 810
BsiHKCI CYCGRG 1 cut(s) 837
BslFI GGGAC 3 cut(s) 152, 634, 767
BslI CCNNNNNNNGG 5 cut(s) 94, 599, 618, 738, 838
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 3 cut(s) 152, 634, 767
BsnI GGCC 6 cut(s) 147, 163, 200, 590, 751, 810
Bso31I GGTCTC 1 cut(s) 860
BsoBI CYCGRG 1 cut(s) 837
Bsp119I TTCGAA 1 cut(s) 635
Bsp120I GGGCCC 1 cut(s) 588
Bsp1286I GDGCHC 3 cut(s) 592, 838, 876
Bsp1407I TGTACA 1 cut(s) 154
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 6 cut(s) 27, 240, 243, 479, 515, 626
BspANI GGCC 6 cut(s) 147, 163, 200, 590, 751, 810
BspCNI CTCAG 3 cut(s) 36, 112, 856
BspLI GGNNCC 6 cut(s) 168, 174, 483, 590, 591, 783
BspMI ACCTGC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 860
BsrBI CCGCTC 1 cut(s) 515
BsrFI RCCGGY 6 cut(s) 163, 738, 747, 762, 778, 806
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 5 cut(s) 110, 284, 341, 485, 711
BssAI RCCGGY 6 cut(s) 163, 738, 747, 762, 778, 806
BssECI CCNNGG 2 cut(s) 57, 88
BssNI GRCGYC 2 cut(s) 276, 623
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 871
BstACI GRCGYC 2 cut(s) 276, 623
BstAUI TGTACA 1 cut(s) 154
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 694
BstDEI CTNAG 6 cut(s) 23, 120, 214, 263, 801, 864
BstEII GGTNACC 1 cut(s) 97
BstF5I GGATG 4 cut(s) 46, 52, 98, 541
BstHHI GCGC 1 cut(s) 578
BstMAI GTCTC 1 cut(s) 860
BstMWI GCNNNNNNNGC 2 cut(s) 242, 748
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 71
BstPI GGTNACC 1 cut(s) 97
BstSCI CCNGG 5 cut(s) 88, 142, 168, 783, 831
BstSLI GKGCMC 2 cut(s) 592, 876
BstV1I GCAGC 3 cut(s) 100, 111, 223
BsuRI GGCC 6 cut(s) 147, 163, 200, 590, 751, 810
BtgZI GCGATG 2 cut(s) 364, 507
BtsCI GGATG 4 cut(s) 46, 52, 98, 541
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 332
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 694
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 6 cut(s) 163, 738, 747, 762, 778, 806
CpoI CGGWCCG 1 cut(s) 829
CseI GACGC 3 cut(s) 276, 284, 631
Csp6I GTAC 3 cut(s) 52, 155, 344
CspI CGGWCCG 1 cut(s) 829
CviAII CATG 1 cut(s) 68
CviQI GTAC 3 cut(s) 52, 155, 344
DdeI CTNAG 6 cut(s) 23, 120, 214, 263, 801, 864
DrdI GACNNNNNNGTC 1 cut(s) 811
DriI GACNNNNNGTC 1 cut(s) 324
DseDI GACNNNNNNGTC 1 cut(s) 811
Eam1105I GACNNNNNGTC 1 cut(s) 324
Eco24I GRGCYC 2 cut(s) 592, 838
Eco31I GGTCTC 1 cut(s) 860
Eco47I GGWCC 5 cut(s) 166, 172, 482, 781, 829
Eco88I CYCGRG 1 cut(s) 837
Eco91I GGTNACC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 589
EcoO65I GGTNACC 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 88
EcoT38I GRGCYC 2 cut(s) 592, 838
FaeI CATG 1 cut(s) 71
FaiI YATR 5 cut(s) 69, 718, 760, 884, 886
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 152, 634, 767
FatI CATG 1 cut(s) 67
FauI CCCGC 2 cut(s) 472, 508
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 7 cut(s) 28, 114, 125, 237, 240, 243, 626
FokI GGATG 4 cut(s) 53, 59, 105, 548
FriOI GRGCYC 2 cut(s) 592, 838
Fsp4HI GCNGC 7 cut(s) 28, 114, 125, 237, 240, 243, 626
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 577
GluI GCNGC 7 cut(s) 28, 114, 125, 237, 240, 243, 626
GsaI CCCAGC 2 cut(s) 596, 879
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 6 cut(s) 147, 163, 200, 590, 751, 810
HgaI GACGC 3 cut(s) 276, 284, 631
HhaI GCGC 1 cut(s) 578
Hin1I GRCGYC 2 cut(s) 276, 623
Hin1II CATG 1 cut(s) 71
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 251
HindII GTYRAC 1 cut(s) 251
HinfI GANTC 2 cut(s) 252, 674
HphI GGTGA 4 cut(s) 109, 273, 581, 665
Hpy166II GTNNAC 2 cut(s) 157, 251
Hpy188I TCNGA 8 cut(s) 35, 58, 186, 305, 512, 679, 723, 865
Hpy188III TCNNGA 2 cut(s) 119, 406
Hpy8I GTNNAC 2 cut(s) 157, 251
Hpy99I CGWCG 4 cut(s) 10, 252, 278, 602
HpyAV CCTTC 5 cut(s) 467, 524, 610, 631, 635
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 2 cut(s) 389, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 748
HpyF3I CTNAG 6 cut(s) 23, 120, 214, 263, 801, 864
HpySE526I ACGT 1 cut(s) 701
Hsp92I GRCGYC 2 cut(s) 276, 623
Hsp92II CATG 1 cut(s) 71
HspAI GCGC 1 cut(s) 576
LmnI GCTCC 3 cut(s) 121, 455, 512
Lsp1109I GCAGC 3 cut(s) 100, 111, 223
LweI GCATC 2 cut(s) 64, 279
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 3 cut(s) 97, 217, 496
MbiI CCGCTC 1 cut(s) 515
MboII GAAGA 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 592, 838, 876
MluCI AATT 4 cut(s) 180, 348, 401, 789
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 3 cut(s) 328, 532, 701
MseI TTAA 2 cut(s) 63, 795
MslI CAYNNNNRTG 1 cut(s) 72
MspA1I CMGCKG 2 cut(s) 27, 628
MspR9I CCNGG 5 cut(s) 90, 144, 170, 785, 833
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 242, 748
NciI CCSGG 4 cut(s) 144, 170, 785, 833
NlaIII CATG 1 cut(s) 71
NlaIV GGNNCC 6 cut(s) 168, 174, 483, 590, 591, 783
NmuCI GTSAC 3 cut(s) 97, 217, 496
NspI RCATGY 1 cut(s) 71
NspV TTCGAA 1 cut(s) 635
PfeI GAWTC 1 cut(s) 674
PfoI TCCNGGA 2 cut(s) 168, 783
PkrI GCNGC 7 cut(s) 29, 115, 126, 238, 241, 244, 627
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
Psp1406I AACGTT 1 cut(s) 701
Psp6I CCWGG 1 cut(s) 88
PspEI GGTNACC 1 cut(s) 97
PspFI CCCAGC 2 cut(s) 592, 875
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 6 cut(s) 168, 174, 483, 590, 591, 783
PspOMI GGGCCC 1 cut(s) 588
RsaI GTAC 3 cut(s) 53, 156, 345
RsaNI GTAC 3 cut(s) 52, 155, 344
RseI CAYNNNNRTG 1 cut(s) 72
Rsr2I CGGWCCG 1 cut(s) 829
RsrII CGGWCCG 1 cut(s) 829
SalI GTCGAC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 63, 795
SatI GCNGC 7 cut(s) 28, 114, 125, 237, 240, 243, 626
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 5 cut(s) 90, 144, 170, 785, 833
SduI GDGCHC 3 cut(s) 592, 838, 876
SfaNI GCATC 2 cut(s) 64, 279
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 5 cut(s) 166, 172, 482, 781, 829
SmiMI CAYNNNNRTG 1 cut(s) 72
Sse9I AATT 4 cut(s) 180, 348, 401, 789
SsiI CCGC 6 cut(s) 27, 240, 243, 479, 515, 626
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 5 cut(s) 88, 142, 168, 783, 831
TaaI ACNGT 1 cut(s) 871
TaiI ACGT 1 cut(s) 704
TaqI TCGA 3 cut(s) 250, 273, 635
TaqII GACCGA 4 cut(s) 515, 746, 817, 849
TasI AATT 4 cut(s) 180, 348, 401, 789
TatI WGTACW 2 cut(s) 154, 343
TauI GCSGC 4 cut(s) 30, 242, 245, 628
TfiI GAWTC 1 cut(s) 674
Tru1I TTAA 2 cut(s) 63, 795
Tru9I TTAA 2 cut(s) 63, 795
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 3 cut(s) 97, 217, 496
TseI GCWGC 3 cut(s) 113, 124, 236
Tsp45I GTSAC 3 cut(s) 97, 217, 496
TspRI CASTG 1 cut(s) 339
VpaK11BI GGWCC 5 cut(s) 166, 172, 482, 781, 829
XapI RAATTY 4 cut(s) 180, 348, 401, 789
XceI RCATGY 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.