MULE transposase domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 1354, 1498 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1248 |
| Acc36I | ACCTGC | 3 cut(s) | 948, 1354, 1498 |
| AccB1I | GGYRCC | 2 cut(s) | 126, 527 |
| AccI | GTMKAC | 1 cut(s) | 1051 |
| AciI | CCGC | 1 cut(s) | 62 |
| AclWI | GGATC | 5 cut(s) | 302, 904, 930, 1350, 1460 |
| AcoI | YGGCCR | 2 cut(s) | 357, 1107 |
| AcsI | RAATTY | 3 cut(s) | 500, 514, 704 |
| AcuI | CTGAAG | 2 cut(s) | 1317, 1461 |
| AcyI | GRCGYC | 1 cut(s) | 1575 |
| AdeI | CACNNNGTG | 1 cut(s) | 586 |
| AfaI | GTAC | 5 cut(s) | 66, 458, 794, 804, 1025 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 766, 919 |
| AflIII | ACRYGT | 3 cut(s) | 816, 1029, 1397 |
| AgsI | TTSAA | 7 cut(s) | 104, 193, 233, 242, 704, 1008, 1100 |
| AjnI | CCWGG | 1 cut(s) | 523 |
| AleI | CACNNNNGTG | 2 cut(s) | 379, 1512 |
| AluBI | AGCT | 6 cut(s) | 442, 624, 853, 1103, 1286, 1427 |
| AluI | AGCT | 6 cut(s) | 442, 624, 853, 1103, 1286, 1427 |
| Alw21I | GWGCWC | 1 cut(s) | 970 |
| Alw26I | GTCTC | 5 cut(s) | 312, 472, 734, 1137, 1566 |
| AlwI | GGATC | 5 cut(s) | 302, 904, 930, 1350, 1460 |
| AoxI | GGCC | 4 cut(s) | 13, 84, 357, 1107 |
| ApoI | RAATTY | 3 cut(s) | 500, 514, 704 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1095 |
| AspS9I | GGNCC | 1 cut(s) | 1161 |
| AsuC2I | CCSGG | 1 cut(s) | 1531 |
| AsuHPI | GGTGA | 1 cut(s) | 838 |
| AvaII | GGWCC | 1 cut(s) | 1161 |
| BaeGI | GKGCMC | 1 cut(s) | 1536 |
| BalI | TGGCCA | 1 cut(s) | 359 |
| BanI | GGYRCC | 2 cut(s) | 126, 527 |
| BbsI | GAAGAC | 3 cut(s) | 762, 1196, 1439 |
| Bbv12I | GWGCWC | 1 cut(s) | 970 |
| BccI | CCATC | 4 cut(s) | 53, 138, 656, 873 |
| BceAI | ACGGC | 2 cut(s) | 515, 713 |
| BciT130I | CCWGG | 1 cut(s) | 525 |
| BcnI | CCSGG | 1 cut(s) | 1531 |
| BcoDI | GTCTC | 5 cut(s) | 312, 472, 734, 1137, 1566 |
| BfaI | CTAG | 1 cut(s) | 1203 |
| BfmI | CTRYAG | 1 cut(s) | 1510 |
| BfuAI | ACCTGC | 3 cut(s) | 948, 1354, 1498 |
| Bme1390I | CCNGG | 2 cut(s) | 525, 1531 |
| Bme18I | GGWCC | 1 cut(s) | 1161 |
| BmgT120I | GGNCC | 1 cut(s) | 1161 |
| BmiI | GGNNCC | 4 cut(s) | 128, 529, 1200, 1528 |
| BmrFI | CCNGG | 2 cut(s) | 525, 1531 |
| BmsI | GCATC | 5 cut(s) | 42, 102, 197, 1006, 1538 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1326 |
| BpiI | GAAGAC | 3 cut(s) | 762, 1196, 1439 |
| BpuMI | CCSGG | 1 cut(s) | 1531 |
| BsaHI | GRCGYC | 1 cut(s) | 1575 |
| BsaI | GGTCTC | 1 cut(s) | 1137 |
| BsaJI | CCNNGG | 1 cut(s) | 1231 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1183, 1213 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 766, 919 |
| Bse3DI | GCAATG | 2 cut(s) | 1303, 1468 |
| BseBI | CCWGG | 1 cut(s) | 525 |
| BseDI | CCNNGG | 1 cut(s) | 1231 |
| BseGI | GGATG | 1 cut(s) | 502 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 766, 919 |
| BseMI | GCAATG | 2 cut(s) | 1303, 1468 |
| BseMII | CTCAG | 6 cut(s) | 171, 467, 723, 1278, 1419, 1581 |
| BseRI | GAGGAG | 1 cut(s) | 1461 |
| BseSI | GKGCMC | 1 cut(s) | 1536 |
| BseYI | CCCAGC | 1 cut(s) | 1115 |
| BsgI | GTGCAG | 1 cut(s) | 771 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1211 |
| BshFI | GGCC | 4 cut(s) | 15, 86, 359, 1109 |
| BshNI | GGYRCC | 2 cut(s) | 126, 527 |
| BsiEI | CGRYCG | 1 cut(s) | 1211 |
| BsiHKAI | GWGCWC | 1 cut(s) | 970 |
| BsiSI | CCGG | 3 cut(s) | 116, 1106, 1530 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 766, 919 |
| BsmAI | GTCTC | 5 cut(s) | 312, 472, 734, 1137, 1566 |
| BsmBI | CGTCTC | 1 cut(s) | 1566 |
| BsmI | GAATGC | 2 cut(s) | 335, 986 |
| BsnI | GGCC | 4 cut(s) | 15, 86, 359, 1109 |
| Bso31I | GGTCTC | 1 cut(s) | 1137 |
| Bsp1286I | GDGCHC | 2 cut(s) | 970, 1536 |
| Bsp1407I | TGTACA | 1 cut(s) | 456 |
| BspACI | CCGC | 1 cut(s) | 62 |
| BspANI | GGCC | 4 cut(s) | 15, 86, 359, 1109 |
| BspCNI | CTCAG | 6 cut(s) | 172, 468, 724, 1279, 1420, 1582 |
| BspHI | TCATGA | 2 cut(s) | 280, 655 |
| BspLI | GGNNCC | 4 cut(s) | 128, 529, 1200, 1528 |
| BspMI | ACCTGC | 3 cut(s) | 948, 1354, 1498 |
| BspPI | GGATC | 5 cut(s) | 302, 904, 930, 1350, 1460 |
| BspQI | GCTCTTC | 1 cut(s) | 1286 |
| BspT107I | GGYRCC | 2 cut(s) | 126, 527 |
| BspTNI | GGTCTC | 1 cut(s) | 1137 |
| BsrDI | GCAATG | 2 cut(s) | 1303, 1468 |
| BsrGI | TGTACA | 1 cut(s) | 456 |
| BssECI | CCNNGG | 1 cut(s) | 1231 |
| BssNI | GRCGYC | 1 cut(s) | 1575 |
| BssT1I | CCWWGG | 1 cut(s) | 1231 |
| Bst2UI | CCWGG | 1 cut(s) | 525 |
| Bst6I | CTCTTC | 2 cut(s) | 571, 1286 |
| BstACI | GRCGYC | 1 cut(s) | 1575 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1346 |
| BstAUI | TGTACA | 1 cut(s) | 456 |
| BstC8I | GCNNGC | 1 cut(s) | 1519 |
| BstDEI | CTNAG | 6 cut(s) | 180, 476, 732, 1287, 1428, 1590 |
| BstF5I | GGATG | 1 cut(s) | 502 |
| BstMAI | GTCTC | 5 cut(s) | 312, 472, 734, 1137, 1566 |
| BstMCI | CGRYCG | 1 cut(s) | 1211 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 974, 1346, 1490 |
| BstNI | CCWGG | 1 cut(s) | 525 |
| BstNSI | RCATGY | 3 cut(s) | 820, 1033, 1401 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1326 |
| BstSCI | CCNGG | 2 cut(s) | 523, 1529 |
| BstSFI | CTRYAG | 1 cut(s) | 1510 |
| BstSLI | GKGCMC | 1 cut(s) | 1536 |
| BstV2I | GAAGAC | 3 cut(s) | 762, 1196, 1439 |
| BstX2I | RGATCY | 2 cut(s) | 909, 1355 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1238 |
| BstYI | RGATCY | 2 cut(s) | 909, 1355 |
| BsuRI | GGCC | 4 cut(s) | 15, 86, 359, 1109 |
| BtsCI | GGATG | 1 cut(s) | 502 |
| BtsIMutI | CAGTG | 5 cut(s) | 714, 754, 863, 1317, 1507 |
| BveI | ACCTGC | 3 cut(s) | 948, 1354, 1498 |
| Cac8I | GCNNGC | 1 cut(s) | 1519 |
| CciI | TCATGA | 2 cut(s) | 280, 655 |
| Cfr13I | GGNCC | 1 cut(s) | 1161 |
| CseI | GACGC | 2 cut(s) | 899, 1583 |
| Csp6I | GTAC | 5 cut(s) | 65, 457, 793, 803, 1024 |
| CviQI | GTAC | 5 cut(s) | 65, 457, 793, 803, 1024 |
| DdeI | CTNAG | 6 cut(s) | 180, 476, 732, 1287, 1428, 1590 |
| DraIII | CACNNNGTG | 1 cut(s) | 586 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1248 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1248 |
| EaeI | YGGCCR | 2 cut(s) | 357, 1107 |
| Eam1104I | CTCTTC | 2 cut(s) | 571, 1286 |
| EarI | CTCTTC | 2 cut(s) | 571, 1286 |
| Eco130I | CCWWGG | 1 cut(s) | 1231 |
| Eco31I | GGTCTC | 1 cut(s) | 1137 |
| Eco47I | GGWCC | 1 cut(s) | 1161 |
| Eco57I | CTGAAG | 2 cut(s) | 1317, 1461 |
| EcoRI | GAATTC | 1 cut(s) | 500 |
| EcoRII | CCWGG | 1 cut(s) | 523 |
| EcoT14I | CCWWGG | 1 cut(s) | 1231 |
| ErhI | CCWWGG | 1 cut(s) | 1231 |
| Esp3I | CGTCTC | 1 cut(s) | 1566 |
| FblI | GTMKAC | 1 cut(s) | 1051 |
| FokI | GGATG | 1 cut(s) | 509 |
| FspBI | CTAG | 1 cut(s) | 1203 |
| GsaI | CCCAGC | 1 cut(s) | 1119 |
| HaeIII | GGCC | 4 cut(s) | 15, 86, 359, 1109 |
| HapII | CCGG | 3 cut(s) | 116, 1106, 1530 |
| HgaI | GACGC | 2 cut(s) | 899, 1583 |
| Hin1I | GRCGYC | 1 cut(s) | 1575 |
| HinfI | GANTC | 2 cut(s) | 401, 1053 |
| HpaII | CCGG | 3 cut(s) | 116, 1106, 1530 |
| HphI | GGTGA | 1 cut(s) | 838 |
| Hpy166II | GTNNAC | 7 cut(s) | 6, 457, 676, 691, 724, 822, 1052 |
| Hpy188I | TCNGA | 3 cut(s) | 181, 225, 1591 |
| Hpy188III | TCNNGA | 7 cut(s) | 49, 281, 298, 488, 613, 656, 1380 |
| Hpy8I | GTNNAC | 7 cut(s) | 6, 457, 676, 691, 724, 822, 1052 |
| HpyAV | CCTTC | 5 cut(s) | 187, 1033, 1264, 1341, 1485 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 974, 1346, 1490 |
| HpyF3I | CTNAG | 6 cut(s) | 180, 476, 732, 1287, 1428, 1590 |
| Hsp92I | GRCGYC | 1 cut(s) | 1575 |
| LguI | GCTCTTC | 1 cut(s) | 1286 |
| LmnI | GCTCC | 5 cut(s) | 439, 644, 1108, 1204, 1526 |
| LweI | GCATC | 5 cut(s) | 42, 102, 197, 1006, 1538 |
| MaeI | CTAG | 1 cut(s) | 1203 |
| MaeIII | GTNAC | 4 cut(s) | 77, 532, 812, 861 |
| MboII | GAAGA | 7 cut(s) | 269, 558, 671, 767, 1201, 1303, 1444 |
| MfeI | CAATTG | 3 cut(s) | 99, 989, 1062 |
| MflI | RGATCY | 2 cut(s) | 909, 1355 |
| MhlI | GDGCHC | 2 cut(s) | 970, 1536 |
| MlsI | TGGCCA | 1 cut(s) | 359 |
| MluCI | AATT | 8 cut(s) | 99, 236, 500, 514, 704, 989, 1062, 1155 |
| MluNI | TGGCCA | 1 cut(s) | 359 |
| MlyI | GAGTC | 1 cut(s) | 1047 |
| Mox20I | TGGCCA | 1 cut(s) | 359 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1095 |
| MscI | TGGCCA | 1 cut(s) | 359 |
| MseI | TTAA | 4 cut(s) | 173, 201, 264, 591 |
| MslI | CAYNNNNRTG | 6 cut(s) | 50, 353, 379, 660, 827, 1512 |
| Msp20I | TGGCCA | 1 cut(s) | 359 |
| MspI | CCGG | 3 cut(s) | 116, 1106, 1530 |
| MspR9I | CCNGG | 2 cut(s) | 525, 1531 |
| MunI | CAATTG | 3 cut(s) | 99, 989, 1062 |
| Mva1269I | GAATGC | 2 cut(s) | 335, 986 |
| MvaI | CCWGG | 1 cut(s) | 525 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 974, 1346, 1490 |
| NciI | CCSGG | 1 cut(s) | 1531 |
| NlaIV | GGNNCC | 4 cut(s) | 128, 529, 1200, 1528 |
| NmuCI | GTSAC | 3 cut(s) | 77, 812, 861 |
| NspI | RCATGY | 3 cut(s) | 820, 1033, 1401 |
| OliI | CACNNNNGTG | 2 cut(s) | 379, 1512 |
| PagI | TCATGA | 2 cut(s) | 280, 655 |
| PaqCI | CACCTGC | 2 cut(s) | 1354, 1498 |
| PciI | ACATGT | 3 cut(s) | 816, 1029, 1397 |
| PciSI | GCTCTTC | 1 cut(s) | 1286 |
| PctI | GAATGC | 2 cut(s) | 335, 986 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1095 |
| PfeI | GAWTC | 1 cut(s) | 401 |
| PleI | GAGTC | 1 cut(s) | 1047 |
| PpsI | GAGTC | 1 cut(s) | 1047 |
| PscI | ACATGT | 3 cut(s) | 816, 1029, 1397 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1326 |
| Psp6I | CCWGG | 1 cut(s) | 523 |
| PspFI | CCCAGC | 1 cut(s) | 1115 |
| PspGI | CCWGG | 1 cut(s) | 523 |
| PspN4I | GGNNCC | 4 cut(s) | 128, 529, 1200, 1528 |
| PspPI | GGNCC | 1 cut(s) | 1161 |
| PsuI | RGATCY | 2 cut(s) | 909, 1355 |
| RsaI | GTAC | 5 cut(s) | 66, 458, 794, 804, 1025 |
| RsaNI | GTAC | 5 cut(s) | 65, 457, 793, 803, 1024 |
| RseI | CAYNNNNRTG | 6 cut(s) | 50, 353, 379, 660, 827, 1512 |
| SapI | GCTCTTC | 1 cut(s) | 1286 |
| SaqAI | TTAA | 4 cut(s) | 173, 201, 264, 591 |
| Sau96I | GGNCC | 1 cut(s) | 1161 |
| SchI | GAGTC | 1 cut(s) | 1047 |
| ScrFI | CCNGG | 2 cut(s) | 525, 1531 |
| SduI | GDGCHC | 2 cut(s) | 970, 1536 |
| SfaNI | GCATC | 5 cut(s) | 42, 102, 197, 1006, 1538 |
| SfcI | CTRYAG | 1 cut(s) | 1510 |
| SinI | GGWCC | 1 cut(s) | 1161 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 50, 353, 379, 660, 827, 1512 |
| Sse9I | AATT | 8 cut(s) | 99, 236, 500, 514, 704, 989, 1062, 1155 |
| SsiI | CCGC | 1 cut(s) | 62 |
| SspMI | CTAG | 1 cut(s) | 1203 |
| StyD4I | CCNGG | 2 cut(s) | 523, 1529 |
| StyI | CCWWGG | 1 cut(s) | 1231 |
| TaqI | TCGA | 2 cut(s) | 37, 870 |
| TaqII | GACCGA | 1 cut(s) | 1225 |
| TasI | AATT | 8 cut(s) | 99, 236, 500, 514, 704, 989, 1062, 1155 |
| TatI | WGTACW | 2 cut(s) | 456, 792 |
| TfiI | GAWTC | 1 cut(s) | 401 |
| Tru1I | TTAA | 4 cut(s) | 173, 201, 264, 591 |
| Tru9I | TTAA | 4 cut(s) | 173, 201, 264, 591 |
| TscAI | CASTG | 5 cut(s) | 721, 754, 870, 1324, 1514 |
| TseFI | GTSAC | 3 cut(s) | 77, 812, 861 |
| Tsp45I | GTSAC | 3 cut(s) | 77, 812, 861 |
| TspRI | CASTG | 5 cut(s) | 721, 754, 870, 1324, 1514 |
| VpaK11BI | GGWCC | 1 cut(s) | 1161 |
| XapI | RAATTY | 3 cut(s) | 500, 514, 704 |
| XceI | RCATGY | 3 cut(s) | 820, 1033, 1401 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 943 |
| XmiI | GTMKAC | 1 cut(s) | 1051 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1095 |
| XspI | CTAG | 1 cut(s) | 1203 |