RLG00000022552

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000607
Physical Location & Seq
Forward (+)
3581141 .. 3583686
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022552

Sequence Viewer

Length: 462 bp
ATGTTGGCCAAAGGTATTATTGGTCCTTTCACTGTGGTGTGCAAGATGTCTTCACTGGAAGAAACACCCCCCATTGTGAACTGGGATAAGAAGAAGCCTAGTCTCGATATTGCAGGTAGCCTTATGGTCCCCGGATTGGATGAAAACCATAAGCATATCTCTGATGAATATGAGAAGGAAGACGACTCAAAGCCAATACATGCGCATACATGTTCTGCTGTTGAAGAAGGATTTTGCATCCATATCACCGATGTCACTCAAAGACCATGCGGCACATGCGCACACCTGTACAACGTCGGTCCAGACAACAAAAAGATATGCAGTTGTATCTTATGCCACTGGGAAGAGGAACAAGATGGGAATGCCAATTCACCTCCCTCAGGTTATTGGTGGTGGCCTAGTGACTCTGAATTCTCTCCAGATATTGATGATTTTCACCAAGCAAGTGTTCTGTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.16

Weight (kDa)

4.73

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 204, 280
Acc36I ACCTGC 1 cut(s) 104
AciI CCGC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 410
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 2 cut(s) 136, 380
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 1 cut(s) 224
AjuI GAANNNNNNNTTGG 1 cut(s) 432
AleI CACNNNNGTG 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 107
AoxI GGCC 2 cut(s) 6, 395
ApoI RAATTY 1 cut(s) 410
AspLEI GCGC 2 cut(s) 205, 281
AspS9I GGNCC 3 cut(s) 23, 127, 299
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 3 cut(s) 238, 363, 428
AvaII GGWCC 3 cut(s) 23, 127, 299
AxyI CCTNAGG 1 cut(s) 379
BalI TGGCCA 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 42, 186
BccI CCATC 1 cut(s) 350
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 2 cut(s) 99, 399
BfuAI ACCTGC 1 cut(s) 104
BisI GCNGC 1 cut(s) 271
BlsI GCNGC 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 3 cut(s) 23, 127, 299
BmgT120I GGNCC 3 cut(s) 23, 127, 299
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 2 cut(s) 91, 349
BmsI GCATC 1 cut(s) 246
BmuI ACTGGG 2 cut(s) 91, 349
BpiI GAAGAC 2 cut(s) 42, 186
BpmI CTGGAG 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 380
Bse1I ACTGG 3 cut(s) 60, 86, 344
Bse21I CCTNAGG 1 cut(s) 379
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 3 cut(s) 145, 237, 462
BseLI CCNNNNNNNGG 2 cut(s) 136, 380
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 3 cut(s) 60, 86, 344
BshFI GGCC 2 cut(s) 8, 397
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 136, 380
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 8, 397
Bsp1407I TGTACA 1 cut(s) 288
BspACI CCGC 1 cut(s) 270
BspANI GGCC 2 cut(s) 8, 397
BspCNI CTCAG 1 cut(s) 392
BspLI GGNNCC 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 3 cut(s) 60, 86, 344
BssECI CCNNGG 1 cut(s) 130
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 288
BstDEI CTNAG 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 378
BstF5I GGATG 3 cut(s) 145, 237, 462
BstHHI GCGC 2 cut(s) 205, 281
BstMAI GTCTC 1 cut(s) 107
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNSI RCATGY 3 cut(s) 203, 213, 279
BstSCI CCNGG 1 cut(s) 130
BstV2I GAAGAC 2 cut(s) 42, 186
Bsu36I CCTNAGG 1 cut(s) 379
BsuRI GGCC 2 cut(s) 8, 397
BtsCI GGATG 3 cut(s) 145, 237, 462
BtsIMutI CAGTG 3 cut(s) 30, 53, 337
BveI ACCTGC 1 cut(s) 104
CfoI GCGC 2 cut(s) 205, 281
Cfr13I GGNCC 3 cut(s) 23, 127, 299
Csp6I GTAC 1 cut(s) 289
CviAII CATG 4 cut(s) 200, 210, 267, 276
CviJI RGCY 5 cut(s) 8, 97, 120, 193, 397
CviKI_1 RGCY 5 cut(s) 8, 97, 120, 193, 397
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco47I GGWCC 3 cut(s) 23, 127, 299
Eco81I CCTNAGG 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 410
FaeI CATG 4 cut(s) 203, 213, 270, 279
FaqI GGGAC 1 cut(s) 113
FatI CATG 4 cut(s) 199, 209, 266, 275
Fnu4HI GCNGC 1 cut(s) 271
FokI GGATG 2 cut(s) 152, 224
Fsp4HI GCNGC 1 cut(s) 271
FspAI RTGCGCAY 2 cut(s) 204, 280
FspBI CTAG 2 cut(s) 99, 399
FspI TGCGCA 2 cut(s) 204, 280
GlaI GCGC 2 cut(s) 204, 280
GluI GCNGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 8, 397
HapII CCGG 1 cut(s) 132
HhaI GCGC 2 cut(s) 205, 281
Hin1II CATG 4 cut(s) 203, 213, 270, 279
Hin6I GCGC 2 cut(s) 203, 279
HinP1I GCGC 2 cut(s) 203, 279
HinfI GANTC 2 cut(s) 185, 404
HpaII CCGG 1 cut(s) 132
HphI GGTGA 3 cut(s) 238, 363, 428
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 163, 409
Hpy188III TCNNGA 3 cut(s) 104, 302, 419
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 169, 221
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 4 cut(s) 42, 113, 237, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 4 cut(s) 203, 213, 270, 279
HspAI GCGC 2 cut(s) 203, 279
LpnPI CCDG 9 cut(s) 41, 67, 99, 145, 299, 315, 325, 366, 432
LweI GCATC 1 cut(s) 246
MaeI CTAG 2 cut(s) 99, 399
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 253, 401
MboII GAAGA 6 cut(s) 42, 71, 103, 191, 236, 356
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 2 cut(s) 367, 410
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 2 cut(s) 179, 398
MnlI CCTC 3 cut(s) 340, 384, 388
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 8
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 276
NciI CCSGG 1 cut(s) 132
NlaIII CATG 4 cut(s) 203, 213, 270, 279
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 253, 401
NsbI TGCGCA 2 cut(s) 204, 280
NspI RCATGY 3 cut(s) 203, 213, 279
OliI CACNNNNGTG 1 cut(s) 35
PciI ACATGT 1 cut(s) 209
PctI GAATGC 1 cut(s) 367
PkrI GCNGC 1 cut(s) 272
PleI GAGTC 2 cut(s) 179, 398
PpsI GAGTC 2 cut(s) 179, 398
PscI ACATGT 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 3 cut(s) 23, 127, 299
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 35
SatI GCNGC 1 cut(s) 271
Sau96I GGNCC 3 cut(s) 23, 127, 299
SchI GAGTC 2 cut(s) 179, 398
ScrFI CCNGG 1 cut(s) 132
SetI ASST 6 cut(s) 16, 118, 288, 297, 376, 385
SfaNI GCATC 1 cut(s) 246
SinI GGWCC 3 cut(s) 23, 127, 299
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 2 cut(s) 367, 410
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 99, 399
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 105
TaqII GACCGA 1 cut(s) 287
TasI AATT 2 cut(s) 367, 410
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 273
TscAI CASTG 3 cut(s) 37, 60, 344
TseFI GTSAC 2 cut(s) 253, 401
Tsp45I GTSAC 2 cut(s) 253, 401
TspDTI ATGAA 2 cut(s) 156, 180
TspRI CASTG 3 cut(s) 37, 60, 344
VpaK11BI GGWCC 3 cut(s) 23, 127, 299
XagI CCTNNNNNAGG 1 cut(s) 378
XapI RAATTY 1 cut(s) 410
XceI RCATGY 3 cut(s) 203, 213, 279
XspI CTAG 2 cut(s) 99, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.