FvH4_4g08102

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000607
Physical Location & Seq
Forward (+)
7815755 .. 7819255
3501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08102.t1

Sequence Viewer

Length: 816 bp
ATGAATATCACTCACACATACCAAAATCCGCATATCTTAGGGGAAGGATGTGTTTGTGGTTGTGAAGAGTTTGTTGCCGCTCCACTCACCCCGGATGTGGATGCCGCCGCCGTGCCACCACTCAAAATTTCAGTGATGATACATCTTGATTGTTGTGTGATTGTGATGACCTCTCTTCTTCTTGTCTCCATTGCACAAGTCACTCTGATCCCTGAAAACCCTTCGATTTTCAAGGGCGCTCTTCATTGTCATGCACAACTAGTGGTACGGGATTCCTCCAACGAAATCGGCGGCCATCTTGATTTTCAAATCCCTGAACGTGATAAAGGAACTTATCTGATTGTGGCCCTCGTTCGCGCCTCCTCCAAAATCTGGTTTGCAGTGGGGGTGGCCGTATCCATTGACGTACAATTGCCCCAGGAGAACTTCACGCCCAACCCAAAGTCGCTTCCTTGGCTTGAGAGATGTGGCGGCTTCGGAGGGGATGGGTACTCCGAGGTTAAGCATGTTGATGCTAAAGCAATCCCAGGATGGGTTCATGGCTCATTAACTCTCTGTGTTCGCGACTTATCAGCTTTTTACAAAGCCCTAGTTTGCATGCACAAAACCCTCGACCAACCCAACGCCTCCAACCTTGAGCGCTGCCCCAAGCGCGTCCAGACGACTCGGTTCTCTTTGGATCCCAAAGCCACCGACAGCGAATCCGACTCTACCCCGATCTGGCTGCGCATAGGCTACCTACACTGCCCTTCTCTCCTCGATCCGACGCGTCTCCACCTTCGATCCAGCAACGGCGCCCAAACCCCGCGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

29.63

Weight (kDa)

6.4

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 728
AccB1I GGYRCC 1 cut(s) 794
AccB7I CCANNNNNTGG 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 80
AccII CGCG 5 cut(s) 357, 564, 654, 769, 808
AciI CCGC 7 cut(s) 29, 78, 105, 108, 291, 471, 806
AclWI GGATC 5 cut(s) 202, 674, 687, 755, 777
AcoI YGGCCR 2 cut(s) 292, 390
AcsI RAATTY 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 3 cut(s) 267, 408, 491
AfeI AGCGCT 1 cut(s) 641
AfiI CCNNNNNNNGG 4 cut(s) 97, 372, 532, 720
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 2 cut(s) 232, 308
AhlI ACTAGT 1 cut(s) 259
AjnI CCWGG 2 cut(s) 417, 526
AluBI AGCT 1 cut(s) 575
AluI AGCT 1 cut(s) 575
Alw26I GTCTC 2 cut(s) 190, 776
AlwI GGATC 5 cut(s) 202, 674, 687, 755, 777
Aor51HI AGCGCT 1 cut(s) 641
AoxI GGCC 3 cut(s) 292, 345, 390
ApeKI GCWGC 2 cut(s) 642, 724
ApoI RAATTY 1 cut(s) 126
AspLEI GCGC 7 cut(s) 239, 359, 642, 654, 729, 797, 810
AspS9I GGNCC 1 cut(s) 346
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 79
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 794
BbvI GCAGC 2 cut(s) 629, 711
BccI CCATC 3 cut(s) 303, 479, 525
BceAI ACGGC 3 cut(s) 95, 377, 808
BcgI CGANNNNNNTGC 2 cut(s) 706, 740
BciT130I CCWGG 2 cut(s) 419, 528
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 190, 776
BcuI ACTAGT 1 cut(s) 259
BfaI CTAG 2 cut(s) 260, 590
BfoI RGCGCY 3 cut(s) 240, 643, 798
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 7 cut(s) 78, 105, 108, 292, 472, 643, 725
BlsI GCNGC 7 cut(s) 79, 106, 109, 293, 473, 644, 726
Bme1390I CCNGG 3 cut(s) 92, 419, 528
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 2 cut(s) 681, 796
BmrFI CCNGG 3 cut(s) 92, 419, 528
BmsI GCATC 2 cut(s) 91, 502
BpuEI CTTGAG 2 cut(s) 479, 656
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 795
BsaJI CCNNGG 5 cut(s) 90, 417, 452, 495, 526
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 372, 532, 720
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 2 cut(s) 419, 528
BseDI CCNNGG 5 cut(s) 90, 417, 452, 495, 526
BseGI GGATG 5 cut(s) 53, 100, 106, 490, 536
BseLI CCNNNNNNNGG 4 cut(s) 97, 372, 532, 720
BseMI GCAATG 1 cut(s) 189
BseRI GAGGAG 2 cut(s) 352, 746
BseXI GCAGC 2 cut(s) 629, 711
Bsh1236I CGCG 5 cut(s) 357, 564, 654, 769, 808
BshFI GGCC 3 cut(s) 294, 347, 392
BshNI GGYRCC 1 cut(s) 794
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 97, 372, 532, 720
BsmAI GTCTC 2 cut(s) 190, 776
BsmBI CGTCTC 1 cut(s) 776
BsnI GGCC 3 cut(s) 294, 347, 392
Bsp143I GATC 5 cut(s) 207, 679, 717, 760, 782
Bsp68I TCGCGA 1 cut(s) 564
BspACI CCGC 7 cut(s) 29, 78, 105, 108, 291, 471, 806
BspANI GGCC 3 cut(s) 294, 347, 392
BspFNI CGCG 5 cut(s) 357, 564, 654, 769, 808
BspLI GGNNCC 2 cut(s) 681, 796
BspPI GGATC 5 cut(s) 202, 674, 687, 755, 777
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 794
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 189
BssECI CCNNGG 5 cut(s) 90, 417, 452, 495, 526
BssMI GATC 5 cut(s) 207, 679, 717, 760, 782
BssNI GRCGYC 1 cut(s) 795
BssT1I CCWWGG 1 cut(s) 452
Bst2UI CCWGG 2 cut(s) 419, 528
Bst6I CTCTTC 3 cut(s) 60, 180, 246
BstACI GRCGYC 1 cut(s) 795
BstC8I GCNNGC 1 cut(s) 599
BstDEI CTNAG 1 cut(s) 37
BstF5I GGATG 5 cut(s) 53, 100, 106, 490, 536
BstFNI CGCG 5 cut(s) 357, 564, 654, 769, 808
BstH2I RGCGCY 3 cut(s) 240, 643, 798
BstHHI GCGC 7 cut(s) 239, 359, 642, 654, 729, 797, 810
BstKTI GATC 5 cut(s) 210, 682, 720, 763, 785
BstMAI GTCTC 2 cut(s) 190, 776
BstMBI GATC 5 cut(s) 207, 679, 717, 760, 782
BstMWI GCNNNNNNNGC 2 cut(s) 454, 651
BstNI CCWGG 2 cut(s) 419, 528
BstNSI RCATGY 2 cut(s) 509, 601
BstSCI CCNGG 3 cut(s) 90, 417, 526
BstUI CGCG 5 cut(s) 357, 564, 654, 769, 808
BstV1I GCAGC 2 cut(s) 629, 711
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 3 cut(s) 294, 347, 392
BtsCI GGATG 5 cut(s) 53, 100, 106, 490, 536
BtsI GCAGTG 2 cut(s) 387, 742
BtsIMutI CAGTG 3 cut(s) 138, 387, 742
BtuMI TCGCGA 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 599
CfoI GCGC 7 cut(s) 239, 359, 642, 654, 729, 797, 810
Cfr13I GGNCC 1 cut(s) 346
CseI GACGC 3 cut(s) 643, 758, 775
Csp6I GTAC 3 cut(s) 266, 407, 490
CviAII CATG 4 cut(s) 251, 506, 539, 598
CviQI GTAC 3 cut(s) 266, 407, 490
DdeI CTNAG 1 cut(s) 37
DinI GGCGCC 1 cut(s) 796
DpnI GATC 5 cut(s) 209, 681, 719, 762, 784
DpnII GATC 5 cut(s) 207, 679, 717, 760, 782
EaeI YGGCCR 2 cut(s) 292, 390
Eam1104I CTCTTC 3 cut(s) 60, 180, 246
EarI CTCTTC 3 cut(s) 60, 180, 246
Eco130I CCWWGG 1 cut(s) 452
Eco47III AGCGCT 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 417, 526
EcoT14I CCWWGG 1 cut(s) 452
EgeI GGCGCC 1 cut(s) 796
EheI GGCGCC 1 cut(s) 796
ErhI CCWWGG 1 cut(s) 452
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 4 cut(s) 254, 509, 542, 601
FaiI YATR 7 cut(s) 19, 33, 252, 507, 540, 599, 731
FatI CATG 4 cut(s) 250, 505, 538, 597
FauI CCCGC 1 cut(s) 813
Fnu4HI GCNGC 7 cut(s) 78, 105, 108, 292, 472, 643, 725
FokI GGATG 5 cut(s) 60, 107, 113, 497, 543
Fsp4HI GCNGC 7 cut(s) 78, 105, 108, 292, 472, 643, 725
FspBI CTAG 2 cut(s) 260, 590
FspI TGCGCA 1 cut(s) 728
GlaI GCGC 7 cut(s) 238, 358, 641, 653, 728, 796, 809
GluI GCNGC 7 cut(s) 78, 105, 108, 292, 472, 643, 725
HaeII RGCGCY 3 cut(s) 240, 643, 798
HaeIII GGCC 3 cut(s) 294, 347, 392
HapII CCGG 1 cut(s) 92
HgaI GACGC 3 cut(s) 643, 758, 775
HhaI GCGC 7 cut(s) 239, 359, 642, 654, 729, 797, 810
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 4 cut(s) 254, 509, 542, 601
Hin6I GCGC 7 cut(s) 237, 357, 640, 652, 727, 795, 808
HinP1I GCGC 7 cut(s) 237, 357, 640, 652, 727, 795, 808
HinfI GANTC 4 cut(s) 272, 664, 701, 707
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 7 cut(s) 207, 339, 479, 496, 706, 765, 815
Hpy188III TCNNGA 4 cut(s) 146, 299, 563, 658
Hpy99I CGWCG 1 cut(s) 769
HpyAV CCTTC 4 cut(s) 38, 231, 759, 788
HpyCH4IV ACGT 2 cut(s) 319, 405
HpyCH4V TGCA 5 cut(s) 194, 254, 380, 597, 601
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 651
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 2 cut(s) 319, 405
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 4 cut(s) 254, 509, 542, 601
HspAI GCGC 7 cut(s) 237, 357, 640, 652, 727, 795, 808
KasI GGCGCC 1 cut(s) 794
Kzo9I GATC 5 cut(s) 207, 679, 717, 760, 782
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 2 cut(s) 629, 711
LweI GCATC 2 cut(s) 91, 502
MaeI CTAG 2 cut(s) 260, 590
MaeII ACGT 2 cut(s) 319, 405
MaeIII GTNAC 1 cut(s) 199
MalI GATC 5 cut(s) 209, 681, 719, 762, 784
MbiI CCGCTC 1 cut(s) 80
MboI GATC 5 cut(s) 207, 679, 717, 760, 782
MboII GAAGA 4 cut(s) 77, 167, 170, 233
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 1 cut(s) 679
MluCI AATT 2 cut(s) 126, 410
MluI ACGCGT 1 cut(s) 767
Mly113I GGCGCC 1 cut(s) 795
MlyI GAGTC 2 cut(s) 658, 701
MmeI TCCRAC 4 cut(s) 303, 654, 729, 788
MseI TTAA 2 cut(s) 501, 548
MslI CAYNNNNRTG 2 cut(s) 249, 510
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 92, 419, 528
MunI CAATTG 1 cut(s) 410
MvaI CCWGG 2 cut(s) 419, 528
MvnI CGCG 5 cut(s) 357, 564, 654, 769, 808
MwoI GCNNNNNNNGC 2 cut(s) 454, 651
NarI GGCGCC 1 cut(s) 795
NciI CCSGG 1 cut(s) 92
NdeII GATC 5 cut(s) 207, 679, 717, 760, 782
NlaIII CATG 4 cut(s) 254, 509, 542, 601
NlaIV GGNNCC 2 cut(s) 681, 796
NmuCI GTSAC 1 cut(s) 199
NruI TCGCGA 1 cut(s) 564
NsbI TGCGCA 1 cut(s) 728
NspI RCATGY 2 cut(s) 509, 601
PaeI GCATGC 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 272, 701
PflMI CCANNNNNTGG 1 cut(s) 372
PkrI GCNGC 7 cut(s) 79, 106, 109, 293, 473, 644, 726
PleI GAGTC 2 cut(s) 658, 701
PluTI GGCGCC 1 cut(s) 798
PpsI GAGTC 2 cut(s) 658, 701
Psp6I CCWGG 2 cut(s) 417, 526
PspGI CCWGG 2 cut(s) 417, 526
PspN4I GGNNCC 2 cut(s) 681, 796
PspPI GGNCC 1 cut(s) 346
PsuI RGATCY 1 cut(s) 679
RruI TCGCGA 1 cut(s) 564
RsaI GTAC 3 cut(s) 267, 408, 491
RsaNI GTAC 3 cut(s) 266, 407, 490
RseI CAYNNNNRTG 2 cut(s) 249, 510
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 501, 548
SatI GCNGC 7 cut(s) 78, 105, 108, 292, 472, 643, 725
Sau3AI GATC 5 cut(s) 207, 679, 717, 760, 782
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 2 cut(s) 658, 701
ScrFI CCNGG 3 cut(s) 92, 419, 528
SetI ASST 8 cut(s) 173, 322, 408, 501, 577, 636, 741, 780
SfaNI GCATC 2 cut(s) 91, 502
SfoI GGCGCC 1 cut(s) 796
SmiMI CAYNNNNRTG 2 cut(s) 249, 510
SmlI CTYRAG 2 cut(s) 458, 635
SmoI CTYRAG 2 cut(s) 458, 635
SpeI ACTAGT 1 cut(s) 259
SphI GCATGC 1 cut(s) 601
Sse9I AATT 2 cut(s) 126, 410
SsiI CCGC 7 cut(s) 29, 78, 105, 108, 291, 471, 806
SspDI GGCGCC 1 cut(s) 794
SspMI CTAG 2 cut(s) 260, 590
StyD4I CCNGG 3 cut(s) 90, 417, 526
StyI CCWWGG 1 cut(s) 452
TaiI ACGT 2 cut(s) 322, 408
TaqI TCGA 4 cut(s) 224, 612, 759, 781
TasI AATT 2 cut(s) 126, 410
TauI GCSGC 5 cut(s) 80, 107, 110, 294, 474
TfiI GAWTC 2 cut(s) 272, 701
Tru1I TTAA 2 cut(s) 501, 548
Tru9I TTAA 2 cut(s) 501, 548
TscAI CASTG 3 cut(s) 138, 387, 749
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 642, 724
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 17, 233, 527
TspRI CASTG 3 cut(s) 138, 387, 749
Van91I CCANNNNNTGG 1 cut(s) 372
XapI RAATTY 1 cut(s) 126
XceI RCATGY 2 cut(s) 509, 601
XspI CTAG 2 cut(s) 260, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.