FvH4_6g47150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000064
Physical Location & Seq
Forward (+)
35813787 .. 35815417
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47150.t1

Sequence Viewer

Length: 672 bp
ATGGGTGGCCGTGGTAAGCGTCGTGTTCCTGCTCAACGTGGTCAACGTCAACTTCCTGCTCAATGTGGTCAACGTCAAGCTCCAGATCCAGGTAATTTTTCTCATCCGGGTCAGGTTTTGGGTCTCAAATGTTACATTCCGGATTATCGCTATTATGGTTCGTGTCATGGATGGCTGATTGCTATGGAGGGGAAAGACTTGTCGAAGAGAGATCCAGAGACAAAAGGGCGTCATCCGAAACAGCTAGGAAGCCGTTTTCAACAGGCACAATCTCATGAGTTGGTGCATTTCTATCAAACTGTTTCGGATGACGCCCTTTTGTCTCTGGATCTCTCTTCTAATTCTGTCACACAGATCTCTACAAGGTTTGATCCTCTTGACTATGAGAAGAAGAATTGGACCCGAAAGAGAACTCTGGGTGATGAAGCACTTTTTGTTGGTGGCAACTCATCTATTTCGGTAACTGCATCAGATATCGAAGGATGCAAACCAGACTCAATATATTTTGTTCGGAATGGGGAAGGGTCATTTGGCACCAGGGCATGGAGATATGATCATGGTGTTTATGATGTGTACATGGGTACCATTTCACAGTTTCCGATTTCCAATTCTACTCTCATGAAGAAGACAAGACAACCTCCAATCTGGGTGTTGCCAACTTTTCAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

224

Amino Acids

25.16

Weight (kDa)

9.37

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 88 - 190 2.7e-14 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 581
AccB1I GGYRCC 2 cut(s) 533, 581
AccB7I CCANNNNNTGG 1 cut(s) 543
AccIII TCCGGA 1 cut(s) 139
AclWI GGATC 4 cut(s) 80, 206, 336, 365
AcoI YGGCCR 1 cut(s) 7
AcyI GRCGYC 2 cut(s) 229, 312
AfaI GTAC 2 cut(s) 575, 583
AfiI CCNNNNNNNGG 2 cut(s) 89, 543
AgsI TTSAA 1 cut(s) 260
AjnI CCWGG 2 cut(s) 88, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
AluBI AGCT 3 cut(s) 80, 244, 667
AluI AGCT 3 cut(s) 80, 244, 667
Alw26I GTCTC 3 cut(s) 128, 212, 327
AlwI GGATC 4 cut(s) 80, 206, 336, 365
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 7
Asp718I GGTACC 1 cut(s) 581
AspS9I GGNCC 1 cut(s) 399
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 399
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BanI GGYRCC 2 cut(s) 533, 581
BbsI GAAGAC 1 cut(s) 632
BccI CCATC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 237
BciT130I CCWGG 2 cut(s) 90, 538
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 128, 212, 327
BfaI CTAG 1 cut(s) 245
BglII AGATCT 1 cut(s) 354
Bme1390I CCNGG 3 cut(s) 90, 108, 538
Bme18I GGWCC 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 3 cut(s) 401, 535, 583
BmrFI CCNGG 3 cut(s) 90, 108, 538
BmsI GCATC 2 cut(s) 473, 476
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 1 cut(s) 66
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 229, 312
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 10, 537
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 543
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 2 cut(s) 90, 538
BseDI CCNNGG 2 cut(s) 10, 537
BseGI GGATG 5 cut(s) 103, 176, 232, 313, 488
BseLI CCNNNNNNNGG 2 cut(s) 89, 543
BshFI GGCC 1 cut(s) 9
BshNI GGYRCC 2 cut(s) 533, 581
BsiSI CCGG 2 cut(s) 107, 140
BslI CCNNNNNNNGG 2 cut(s) 89, 543
BsmAI GTCTC 3 cut(s) 128, 212, 327
BsnI GGCC 1 cut(s) 9
Bso31I GGTCTC 1 cut(s) 128
Bsp13I TCCGGA 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 6 cut(s) 85, 211, 328, 354, 370, 553
BspANI GGCC 1 cut(s) 9
BspEI TCCGGA 1 cut(s) 139
BspHI TCATGA 2 cut(s) 274, 618
BspLI GGNNCC 3 cut(s) 401, 535, 583
BspPI GGATC 4 cut(s) 80, 206, 336, 365
BspT107I GGYRCC 2 cut(s) 533, 581
BspTNI GGTCTC 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 573
BssECI CCNNGG 2 cut(s) 10, 537
BssMI GATC 6 cut(s) 85, 211, 328, 354, 370, 553
BssNI GRCGYC 2 cut(s) 229, 312
Bst2UI CCWGG 2 cut(s) 90, 538
Bst4CI ACNGT 2 cut(s) 301, 594
Bst6I CTCTTC 2 cut(s) 200, 340
BstACI GRCGYC 2 cut(s) 229, 312
BstAUI TGTACA 1 cut(s) 573
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 5 cut(s) 103, 176, 232, 313, 488
BstKTI GATC 6 cut(s) 88, 214, 331, 357, 373, 556
BstMAI GTCTC 3 cut(s) 128, 212, 327
BstMBI GATC 6 cut(s) 85, 211, 328, 354, 370, 553
BstNI CCWGG 2 cut(s) 90, 538
BstSCI CCNGG 3 cut(s) 88, 106, 536
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 4 cut(s) 85, 211, 328, 354
BstYI RGATCY 4 cut(s) 85, 211, 328, 354
BsuRI GGCC 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 5 cut(s) 103, 176, 232, 313, 488
CciI TCATGA 2 cut(s) 274, 618
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 3 cut(s) 8, 218, 320
Csp6I GTAC 2 cut(s) 574, 582
CviAII CATG 6 cut(s) 167, 275, 543, 557, 577, 619
CviJI RGCY 6 cut(s) 9, 80, 175, 244, 252, 667
CviKI_1 RGCY 6 cut(s) 9, 80, 175, 244, 252, 667
CviQI GTAC 2 cut(s) 574, 582
DpnI GATC 6 cut(s) 87, 213, 330, 356, 372, 555
DpnII GATC 6 cut(s) 85, 211, 328, 354, 370, 553
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 2 cut(s) 200, 340
EarI CTCTTC 2 cut(s) 200, 340
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 88, 536
EcoRV GATATC 1 cut(s) 475
FaeI CATG 6 cut(s) 170, 278, 546, 560, 580, 622
FatI CATG 6 cut(s) 166, 274, 542, 556, 576, 618
FbaI TGATCA 1 cut(s) 553
FokI GGATG 5 cut(s) 90, 183, 219, 320, 495
FspBI CTAG 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 1 cut(s) 9
HapII CCGG 2 cut(s) 107, 140
HgaI GACGC 3 cut(s) 8, 218, 320
Hin1I GRCGYC 2 cut(s) 229, 312
Hin1II CATG 6 cut(s) 170, 278, 546, 560, 580, 622
HincII GTYRAC 3 cut(s) 44, 50, 71
HindII GTYRAC 3 cut(s) 44, 50, 71
HinfI GANTC 1 cut(s) 494
HpaII CCGG 2 cut(s) 107, 140
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 4 cut(s) 44, 50, 71, 574
Hpy188I TCNGA 6 cut(s) 237, 307, 472, 513, 600, 671
Hpy188III TCNNGA 7 cut(s) 83, 140, 215, 275, 326, 377, 619
Hpy8I GTNNAC 4 cut(s) 44, 50, 71, 574
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 473, 515
HpyCH4III ACNGT 2 cut(s) 301, 594
HpyCH4IV ACGT 3 cut(s) 37, 46, 73
HpyCH4V TGCA 3 cut(s) 286, 467, 486
HpySE526I ACGT 3 cut(s) 37, 46, 73
Hsp92I GRCGYC 2 cut(s) 229, 312
Hsp92II CATG 6 cut(s) 170, 278, 546, 560, 580, 622
Kpn2I TCCGGA 1 cut(s) 139
KpnI GGTACC 1 cut(s) 585
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 6 cut(s) 85, 211, 328, 354, 370, 553
LmnI GCTCC 1 cut(s) 85
LweI GCATC 2 cut(s) 473, 476
MaeI CTAG 1 cut(s) 245
MaeII ACGT 3 cut(s) 37, 46, 73
MaeIII GTNAC 3 cut(s) 131, 346, 460
MalI GATC 6 cut(s) 87, 213, 330, 356, 372, 555
MboI GATC 6 cut(s) 85, 211, 328, 354, 370, 553
MboII GAAGA 6 cut(s) 217, 327, 400, 403, 634, 637
MflI RGATCY 4 cut(s) 85, 211, 328, 354
MluCI AATT 4 cut(s) 94, 340, 394, 607
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 3 cut(s) 181, 384, 648
MroI TCCGGA 1 cut(s) 139
MspI CCGG 2 cut(s) 107, 140
MspR9I CCNGG 3 cut(s) 90, 108, 538
MvaI CCWGG 2 cut(s) 90, 538
NciI CCSGG 1 cut(s) 108
NdeII GATC 6 cut(s) 85, 211, 328, 354, 370, 553
NlaIII CATG 6 cut(s) 170, 278, 546, 560, 580, 622
NlaIV GGNNCC 3 cut(s) 401, 535, 583
NmuCI GTSAC 1 cut(s) 346
PagI TCATGA 2 cut(s) 274, 618
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PflMI CCANNNNNTGG 1 cut(s) 543
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
Psp6I CCWGG 2 cut(s) 88, 536
PspGI CCWGG 2 cut(s) 88, 536
PspN4I GGNNCC 3 cut(s) 401, 535, 583
PspPI GGNCC 1 cut(s) 399
PsuI RGATCY 4 cut(s) 85, 211, 328, 354
RsaI GTAC 2 cut(s) 575, 583
RsaNI GTAC 2 cut(s) 574, 582
Sau3AI GATC 6 cut(s) 85, 211, 328, 354, 370, 553
Sau96I GGNCC 1 cut(s) 399
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 3 cut(s) 90, 108, 538
SfaNI GCATC 2 cut(s) 473, 476
SinI GGWCC 1 cut(s) 399
Sse9I AATT 4 cut(s) 94, 340, 394, 607
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 3 cut(s) 88, 106, 536
TaaI ACNGT 2 cut(s) 301, 594
TaiI ACGT 3 cut(s) 40, 49, 76
TaqI TCGA 2 cut(s) 203, 477
TasI AATT 4 cut(s) 94, 340, 394, 607
TatI WGTACW 1 cut(s) 573
TseFI GTSAC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 2 cut(s) 438, 635
Van91I CCANNNNNTGG 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 399
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.