Rh2BG073900

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000064
Physical Location & Seq
Forward (+)
5585685 .. 5588118
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG073900.1

Sequence Viewer

Length: 1269 bp
ATGGCTGCTACCTTCCGCCGCAAACAACTATTGGCAGACTGGTCTAGTCTTCCTCATGTCATTCTCTCAGAGATTCTGGATAAGTTGTTGGAACCAACTGACCATGTTCGTTTTGCTGCCGTTTGCAAGGAATGGCATGCTCTCGCAACAGACTATAACCACACGACCCAGCGTTGTTTTAAGGTGCATCCCATGCTTATGATCCCTTCAGAATCCCAAGGAAAGCGAAAACTATACAGCATCTCTGAGCAAAAAGTCTACAACAACTTTGAGTTACCACAAGCTTGTAATAGCACTAAGAGGTGTTGTGGCTCGAGCAATGCTCCTGGTTGGTTGGTCGCAGTAGATCTACTAGGCGAACATCAACTCAGTACAACTCTAGTGAACGTGTTCAAAAGAGAAGTGACGCCCATTCGTCTACCTCCATTGTATTTCAATGCCGAGAGTCATTGGTTCATGGTTTTCTCCCCAAAGGTTCTCTTATCCGCTGATCCAATTGCGAATCCAGACAATTATGTGGTTGTGGCAGTTTATGATAGGGTTTCAAGGATCGCTTTCATAAAAGGAGGACAAACAATCTGGACTTACCTTGAGGTCTACTCCTTCTTTACTGATGCTATCTTTTATAAAAGAAAAGTCTATGCGGTCGGACAATGGGGACGGATTAAATCGTTTGATGTTAATAGCAAGCCAGTAGAAGAAAGTTTACTTGCACATGGACGATATCGGTTTTCATGTCATGCGCATAAGGGGTATCTTGTGGAAACAACTAAAGGAGACTTGTTGCATGTTCGAAGATTTTTGAAACAGAAGGATGTACCACGACATTACTATTTCCATTATGAGCATAATGAGTTCTGGACCGAAAGCTTCAAGGTTTACAAGGTGGTGTTTGATGAGAGGGACGAATCCATTGTGGAGCAGGTTGAGTTAAAGAGTATTGGAGATGAGGCTTTGTTTGTTGGTGACAATCACTCGATGTGTGTTTTGGCTTCAAACTTTCCTGGGTGCCAACCAAATTCCATATACTACACGGATGACTTGATAGAACTTGGAGAAGTGCGATTAGTTGATGATGGATCAATTGATATGGATGACATCTTCAGTTTAATCAAACTAAAGGATGAAACCATCTCTAATGGACCAAGTGATATGGGCATCTTCAATTTAGAGGATGGAAGCCTTACGCAACATTATTCTCTAAATCCTTGGCACAGAAACATCCCACCTGCTATCTGGATCATGCCATTGTTTAATGGACTCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

422

Amino Acids

48.56

Weight (kDa)

6.19

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 14 - 51 1.5e-08 F-box-like
Beta-prop_KIB1-4 PF03478 78 - 388 1e-50 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AarI CACCTGC 1 cut(s) 1237
Acc16I TGCGCA 1 cut(s) 744
Acc36I ACCTGC 2 cut(s) 913, 1237
AccB1I GGYRCC 1 cut(s) 1008
AccI GTMKAC 3 cut(s) 258, 418, 597
AciI CCGC 4 cut(s) 16, 19, 486, 644
AclWI GGATC 5 cut(s) 196, 485, 557, 1087, 1247
AcsI RAATTY 1 cut(s) 1018
AcuI CTGAAG 2 cut(s) 192, 1087
AcyI GRCGYC 1 cut(s) 407
AfaI GTAC 2 cut(s) 373, 819
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 7 cut(s) 394, 436, 546, 805, 874, 996, 1165
AjnI CCWGG 2 cut(s) 325, 1003
AluBI AGCT 2 cut(s) 284, 870
AluI AGCT 2 cut(s) 284, 870
Alw26I GTCTC 1 cut(s) 771
AlwI GGATC 5 cut(s) 196, 485, 557, 1087, 1247
Ama87I CYCGRG 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 5, 116
ApoI RAATTY 1 cut(s) 1018
Asp700I GAANNNNTTC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 2 cut(s) 861, 1142
AsuHPI GGTGA 1 cut(s) 977
AsuII TTCGAA 1 cut(s) 793
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 2 cut(s) 861, 1142
BanI GGYRCC 1 cut(s) 1008
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 103
BccI CCATC 3 cut(s) 1070, 1139, 1169
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 2 cut(s) 327, 1005
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 3 cut(s) 45, 353, 380
BfuAI ACCTGC 2 cut(s) 913, 1237
BglII AGATCT 1 cut(s) 346
BisI GCNGC 3 cut(s) 6, 19, 117
BlsI GCNGC 3 cut(s) 7, 20, 118
Bme1390I CCNGG 2 cut(s) 327, 1005
Bme18I GGWCC 2 cut(s) 861, 1142
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 861, 1142
BmiI GGNNCC 2 cut(s) 93, 1010
BmrFI CCNGG 2 cut(s) 327, 1005
BmsI GCATC 4 cut(s) 196, 249, 604, 1167
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 4 cut(s) 307, 339, 584, 616
Bpu14I TTCGAA 1 cut(s) 793
BpuEI CTTGAG 1 cut(s) 611
BsaHI GRCGYC 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 217, 1004, 1208
Bse1I ACTGG 2 cut(s) 44, 692
Bse3DI GCAATG 1 cut(s) 325
BseBI CCWGG 2 cut(s) 327, 1005
BseDI CCNNGG 3 cut(s) 217, 1004, 1208
BseGI GGATG 7 cut(s) 187, 820, 1042, 1099, 1129, 1180, 1221
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 81, 237, 382
BseNI ACTGG 2 cut(s) 44, 692
BseXI GCAGC 1 cut(s) 103
BseYI CCCAGC 1 cut(s) 168
Bsh1285I CGRYCG 1 cut(s) 648
BshNI GGYRCC 1 cut(s) 1008
BsiEI CGRYCG 1 cut(s) 648
BsiHKCI CYCGRG 1 cut(s) 313
BslFI GGGAC 2 cut(s) 672, 917
BsmAI GTCTC 1 cut(s) 771
BsmFI GGGAC 2 cut(s) 672, 917
BsoBI CYCGRG 1 cut(s) 313
Bsp119I TTCGAA 1 cut(s) 793
Bsp143I GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
BspACI CCGC 4 cut(s) 16, 19, 486, 644
BspCNI CTCAG 3 cut(s) 80, 238, 381
BspLI GGNNCC 2 cut(s) 93, 1010
BspMI ACCTGC 2 cut(s) 913, 1237
BspPI GGATC 5 cut(s) 196, 485, 557, 1087, 1247
BspT104I TTCGAA 1 cut(s) 793
BspT107I GGYRCC 1 cut(s) 1008
BsrDI GCAATG 1 cut(s) 325
BsrI ACTGG 2 cut(s) 44, 692
BssECI CCNNGG 3 cut(s) 217, 1004, 1208
BssMI GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
BssNI GRCGYC 1 cut(s) 407
BssT1I CCWWGG 2 cut(s) 217, 1208
Bst2UI CCWGG 2 cut(s) 327, 1005
BstACI GRCGYC 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 793
BstC8I GCNNGC 2 cut(s) 138, 689
BstDEI CTNAG 4 cut(s) 67, 246, 297, 368
BstF5I GGATG 7 cut(s) 187, 820, 1042, 1099, 1129, 1180, 1221
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 6 cut(s) 204, 349, 493, 552, 1082, 1242
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
BstMCI CGRYCG 1 cut(s) 648
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 2 cut(s) 327, 1005
BstNSI RCATGY 2 cut(s) 140, 791
BstSCI CCNGG 2 cut(s) 325, 1003
BstV1I GCAGC 1 cut(s) 103
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BtsCI GGATG 7 cut(s) 187, 820, 1042, 1099, 1129, 1180, 1221
BveI ACCTGC 2 cut(s) 913, 1237
Cac8I GCNNGC 2 cut(s) 138, 689
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 861, 1142
CseI GACGC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 372, 818
CviJI RGCY 9 cut(s) 5, 284, 312, 691, 870, 953, 992, 1182, 1266
CviKI_1 RGCY 9 cut(s) 5, 284, 312, 691, 870, 953, 992, 1182, 1266
CviQI GTAC 2 cut(s) 372, 818
DdeI CTNAG 4 cut(s) 67, 246, 297, 368
DpnI GATC 6 cut(s) 203, 348, 492, 551, 1081, 1241
DpnII GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
EciI GGCGGA 1 cut(s) 5
Eco130I CCWWGG 2 cut(s) 217, 1208
Eco32I GATATC 1 cut(s) 725
Eco47I GGWCC 2 cut(s) 861, 1142
Eco57I CTGAAG 2 cut(s) 192, 1087
Eco88I CYCGRG 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 325, 1003
EcoRV GATATC 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 217, 1208
ErhI CCWWGG 2 cut(s) 217, 1208
FalI AAGNNNNNCTT 4 cut(s) 464, 496, 538, 570
FaqI GGGAC 2 cut(s) 672, 917
FblI GTMKAC 3 cut(s) 258, 418, 597
Fnu4HI GCNGC 3 cut(s) 6, 19, 117
FokI GGATG 7 cut(s) 174, 827, 1049, 1106, 1136, 1187, 1208
Fsp4HI GCNGC 3 cut(s) 6, 19, 117
FspAI RTGCGCAY 1 cut(s) 744
FspBI CTAG 3 cut(s) 45, 353, 380
FspI TGCGCA 1 cut(s) 744
GlaI GCGC 1 cut(s) 744
GluI GCNGC 3 cut(s) 6, 19, 117
GsaI CCCAGC 1 cut(s) 172
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 745
Hin1I GRCGYC 1 cut(s) 407
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HindIII AAGCTT 2 cut(s) 282, 868
HinfI GANTC 6 cut(s) 73, 212, 445, 502, 908, 1260
HphI GGTGA 1 cut(s) 977
Hpy166II GTNNAC 6 cut(s) 259, 385, 419, 598, 707, 880
Hpy188I TCNGA 4 cut(s) 70, 211, 247, 650
Hpy188III TCNNGA 5 cut(s) 77, 506, 580, 859, 1237
Hpy8I GTNNAC 6 cut(s) 259, 385, 419, 598, 707, 880
HpyAV CCTTC 4 cut(s) 22, 216, 613, 805
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 126, 187, 713, 787
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 4 cut(s) 67, 246, 297, 368
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 407
HspAI GCGC 1 cut(s) 743
Kzo9I GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
LmnI GCTCC 2 cut(s) 328, 919
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 4 cut(s) 196, 249, 604, 1167
MaeI CTAG 3 cut(s) 45, 353, 380
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 273, 403, 965
MalI GATC 6 cut(s) 203, 348, 492, 551, 1081, 1241
MboI GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
MboII GAAGA 5 cut(s) 41, 710, 807, 1093, 1153
MfeI CAATTG 2 cut(s) 495, 1083
MflI RGATCY 1 cut(s) 346
MluCI AATT 5 cut(s) 495, 511, 1018, 1083, 1165
MlyI GAGTC 2 cut(s) 454, 1254
MmeI TCCRAC 2 cut(s) 69, 628
MnlI CCTC 8 cut(s) 63, 294, 432, 560, 586, 894, 943, 1165
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 6 cut(s) 180, 666, 681, 932, 1109, 1254
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 488
MspR9I CCNGG 2 cut(s) 327, 1005
MunI CAATTG 2 cut(s) 495, 1083
MvaI CCWGG 2 cut(s) 327, 1005
MwoI GCNNNNNNNGC 1 cut(s) 193
NdeII GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
NlaIV GGNNCC 2 cut(s) 93, 1010
NmeAIII GCCGAG 2 cut(s) 466, 1242
NmuCI GTSAC 2 cut(s) 403, 965
NsbI TGCGCA 1 cut(s) 744
NspI RCATGY 2 cut(s) 140, 791
NspV TTCGAA 1 cut(s) 793
PaeI GCATGC 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 313
PaqCI CACCTGC 1 cut(s) 1237
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 4 cut(s) 73, 212, 502, 908
PkrI GCNGC 3 cut(s) 7, 20, 118
PleI GAGTC 2 cut(s) 453, 1254
PpsI GAGTC 2 cut(s) 453, 1254
PsiI TTATAA 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 325, 1003
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 2 cut(s) 325, 1003
PspN4I GGNNCC 2 cut(s) 93, 1010
PspPI GGNCC 2 cut(s) 861, 1142
PspXI VCTCGAGB 1 cut(s) 313
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 373, 819
RsaNI GTAC 2 cut(s) 372, 818
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 6 cut(s) 180, 666, 681, 932, 1109, 1254
SatI GCNGC 3 cut(s) 6, 19, 117
Sau3AI GATC 6 cut(s) 201, 346, 490, 549, 1079, 1239
Sau96I GGNCC 2 cut(s) 861, 1142
SchI GAGTC 2 cut(s) 454, 1254
ScrFI CCNGG 2 cut(s) 327, 1005
SfaNI GCATC 4 cut(s) 196, 249, 604, 1167
Sfr274I CTCGAG 1 cut(s) 313
SfuI TTCGAA 1 cut(s) 793
SinI GGWCC 2 cut(s) 861, 1142
SlaI CTCGAG 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 313, 590
SmoI CTYRAG 2 cut(s) 313, 590
SphI GCATGC 1 cut(s) 140
Sse9I AATT 5 cut(s) 495, 511, 1018, 1083, 1165
SsiI CCGC 4 cut(s) 16, 19, 486, 644
SspMI CTAG 3 cut(s) 45, 353, 380
StyD4I CCNGG 2 cut(s) 325, 1003
StyI CCWWGG 2 cut(s) 217, 1208
TaiI ACGT 1 cut(s) 390
TaqI TCGA 3 cut(s) 314, 793, 977
TaqII GACCGA 1 cut(s) 878
TasI AATT 5 cut(s) 495, 511, 1018, 1083, 1165
TatI WGTACW 1 cut(s) 371
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 4 cut(s) 73, 212, 502, 908
Tru1I TTAA 6 cut(s) 180, 666, 681, 932, 1109, 1254
Tru9I TTAA 6 cut(s) 180, 666, 681, 932, 1109, 1254
TseFI GTSAC 2 cut(s) 403, 965
TseI GCWGC 2 cut(s) 5, 116
Tsp45I GTSAC 2 cut(s) 403, 965
TspDTI ATGAA 4 cut(s) 445, 547, 723, 1140
TspGWI ACGGA 2 cut(s) 676, 1049
VpaK11BI GGWCC 2 cut(s) 861, 1142
XapI RAATTY 1 cut(s) 1018
XceI RCATGY 2 cut(s) 140, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 1233
XhoI CTCGAG 1 cut(s) 313
XmiI GTMKAC 3 cut(s) 258, 418, 597
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 3 cut(s) 45, 353, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.