Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 558 |
| AccIII | TCCGGA | 1 cut(s) | 58 |
| AciI | CCGC | 1 cut(s) | 4 |
| AclWI | GGATC | 2 cut(s) | 38, 229 |
| AcsI | RAATTY | 4 cut(s) | 551, 587, 723, 742 |
| AfaI | GTAC | 3 cut(s) | 97, 102, 752 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 290, 325, 485 |
| AflIII | ACRYGT | 1 cut(s) | 858 |
| AgeI | ACCGGT | 1 cut(s) | 892 |
| AgsI | TTSAA | 3 cut(s) | 55, 369, 592 |
| AluBI | AGCT | 3 cut(s) | 241, 259, 900 |
| AluI | AGCT | 3 cut(s) | 241, 259, 900 |
| Alw26I | GTCTC | 2 cut(s) | 309, 660 |
| AlwI | GGATC | 2 cut(s) | 38, 229 |
| Ama87I | CYCGRG | 1 cut(s) | 406 |
| Aor13HI | TCCGGA | 1 cut(s) | 58 |
| AoxI | GGCC | 2 cut(s) | 124, 193 |
| ApeKI | GCWGC | 1 cut(s) | 640 |
| ApoI | RAATTY | 4 cut(s) | 551, 587, 723, 742 |
| AseI | ATTAAT | 1 cut(s) | 785 |
| AsiGI | ACCGGT | 1 cut(s) | 892 |
| AsuC2I | CCSGG | 1 cut(s) | 776 |
| AsuII | TTCGAA | 1 cut(s) | 547 |
| AvaI | CYCGRG | 1 cut(s) | 406 |
| BbvI | GCAGC | 1 cut(s) | 652 |
| BccI | CCATC | 6 cut(s) | 64, 152, 617, 702, 724, 824 |
| BciVI | GTATCC | 1 cut(s) | 353 |
| BclI | TGATCA | 1 cut(s) | 247 |
| BcnI | CCSGG | 1 cut(s) | 776 |
| BcoDI | GTCTC | 2 cut(s) | 309, 660 |
| BfaI | CTAG | 4 cut(s) | 473, 768, 824, 901 |
| BfuI | GTATCC | 1 cut(s) | 353 |
| BisI | GCNGC | 2 cut(s) | 5, 641 |
| BlsI | GCNGC | 2 cut(s) | 6, 642 |
| Bme1390I | CCNGG | 1 cut(s) | 776 |
| BmeT110I | CYCGRG | 1 cut(s) | 406 |
| BmiI | GGNNCC | 1 cut(s) | 631 |
| BmrFI | CCNGG | 1 cut(s) | 776 |
| BmsI | GCATC | 3 cut(s) | 166, 295, 661 |
| BpmI | CTGGAG | 3 cut(s) | 157, 221, 347 |
| Bpu14I | TTCGAA | 1 cut(s) | 547 |
| BpuMI | CCSGG | 1 cut(s) | 776 |
| BsaI | GGTCTC | 1 cut(s) | 309 |
| BsaJI | CCNNGG | 2 cut(s) | 429, 633 |
| BsaWI | WCCGGW | 2 cut(s) | 58, 892 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 290, 325, 485 |
| Bse118I | RCCGGY | 1 cut(s) | 892 |
| Bse3DI | GCAATG | 1 cut(s) | 343 |
| BseAI | TCCGGA | 1 cut(s) | 58 |
| BseDI | CCNNGG | 2 cut(s) | 429, 633 |
| BseGI | GGATG | 1 cut(s) | 181 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 290, 325, 485 |
| BseMI | GCAATG | 1 cut(s) | 343 |
| BseMII | CTCAG | 1 cut(s) | 141 |
| BseRI | GAGGAG | 5 cut(s) | 446, 449, 452, 455, 458 |
| BseXI | GCAGC | 1 cut(s) | 652 |
| BsgI | GTGCAG | 1 cut(s) | 659 |
| BshFI | GGCC | 2 cut(s) | 126, 195 |
| BshTI | ACCGGT | 1 cut(s) | 892 |
| BsiHKCI | CYCGRG | 1 cut(s) | 406 |
| BsiSI | CCGG | 3 cut(s) | 59, 775, 893 |
| BslFI | GGGAC | 2 cut(s) | 543, 869 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 290, 325, 485 |
| BsmAI | GTCTC | 2 cut(s) | 309, 660 |
| BsmBI | CGTCTC | 1 cut(s) | 660 |
| BsmFI | GGGAC | 2 cut(s) | 543, 869 |
| BsmI | GAATGC | 1 cut(s) | 407 |
| BsnI | GGCC | 2 cut(s) | 126, 195 |
| Bso31I | GGTCTC | 1 cut(s) | 309 |
| BsoBI | CYCGRG | 1 cut(s) | 406 |
| Bsp119I | TTCGAA | 1 cut(s) | 547 |
| Bsp13I | TCCGGA | 1 cut(s) | 58 |
| Bsp143I | GATC | 3 cut(s) | 43, 221, 247 |
| Bsp19I | CCATGG | 1 cut(s) | 633 |
| BspACI | CCGC | 1 cut(s) | 4 |
| BspANI | GGCC | 2 cut(s) | 126, 195 |
| BspCNI | CTCAG | 1 cut(s) | 142 |
| BspEI | TCCGGA | 1 cut(s) | 58 |
| BspLI | GGNNCC | 1 cut(s) | 631 |
| BspPI | GGATC | 2 cut(s) | 38, 229 |
| BspT104I | TTCGAA | 1 cut(s) | 547 |
| BspTNI | GGTCTC | 1 cut(s) | 309 |
| BsrDI | GCAATG | 1 cut(s) | 343 |
| BsrFI | RCCGGY | 1 cut(s) | 892 |
| BssAI | RCCGGY | 1 cut(s) | 892 |
| BssECI | CCNNGG | 2 cut(s) | 429, 633 |
| BssMI | GATC | 3 cut(s) | 43, 221, 247 |
| BssT1I | CCWWGG | 1 cut(s) | 633 |
| Bst4CI | ACNGT | 2 cut(s) | 100, 659 |
| Bst6I | CTCTTC | 2 cut(s) | 430, 564 |
| BstBI | TTCGAA | 1 cut(s) | 547 |
| BstC8I | GCNNGC | 2 cut(s) | 124, 257 |
| BstDEI | CTNAG | 5 cut(s) | 150, 295, 492, 525, 802 |
| BstDSI | CCRYGG | 1 cut(s) | 633 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 483 |
| BstF5I | GGATG | 1 cut(s) | 181 |
| BstKTI | GATC | 3 cut(s) | 46, 224, 250 |
| BstMAI | GTCTC | 2 cut(s) | 309, 660 |
| BstMBI | GATC | 3 cut(s) | 43, 221, 247 |
| BstNSI | RCATGY | 3 cut(s) | 356, 545, 647 |
| BstSCI | CCNGG | 1 cut(s) | 774 |
| BstV1I | GCAGC | 1 cut(s) | 652 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 879 |
| BsuI | GTATCC | 1 cut(s) | 353 |
| BsuRI | GGCC | 2 cut(s) | 126, 195 |
| BtgI | CCRYGG | 1 cut(s) | 633 |
| BtsCI | GGATG | 1 cut(s) | 181 |
| BtsIMutI | CAGTG | 1 cut(s) | 147 |
| Cac8I | GCNNGC | 2 cut(s) | 124, 257 |
| Cfr10I | RCCGGY | 1 cut(s) | 892 |
| Csp6I | GTAC | 3 cut(s) | 96, 101, 751 |
| CspAI | ACCGGT | 1 cut(s) | 892 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 195, 230 |
| CviAII | CATG | 6 cut(s) | 353, 542, 584, 634, 644, 748 |
| CviQI | GTAC | 3 cut(s) | 96, 101, 751 |
| DdeI | CTNAG | 5 cut(s) | 150, 295, 492, 525, 802 |
| DpnI | GATC | 3 cut(s) | 45, 223, 249 |
| DpnII | GATC | 3 cut(s) | 43, 221, 247 |
| DraI | TTTAAA | 1 cut(s) | 84 |
| Eam1104I | CTCTTC | 2 cut(s) | 430, 564 |
| EarI | CTCTTC | 2 cut(s) | 430, 564 |
| Eco130I | CCWWGG | 1 cut(s) | 633 |
| Eco31I | GGTCTC | 1 cut(s) | 309 |
| Eco88I | CYCGRG | 1 cut(s) | 406 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 483 |
| EcoRI | GAATTC | 1 cut(s) | 587 |
| EcoT14I | CCWWGG | 1 cut(s) | 633 |
| ErhI | CCWWGG | 1 cut(s) | 633 |
| Esp3I | CGTCTC | 1 cut(s) | 660 |
| FaeI | CATG | 6 cut(s) | 356, 545, 587, 637, 647, 751 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 495, 527 |
| FaqI | GGGAC | 2 cut(s) | 543, 869 |
| FatI | CATG | 6 cut(s) | 352, 541, 583, 633, 643, 747 |
| FbaI | TGATCA | 1 cut(s) | 247 |
| Fnu4HI | GCNGC | 2 cut(s) | 5, 641 |
| FokI | GGATG | 1 cut(s) | 188 |
| Fsp4HI | GCNGC | 2 cut(s) | 5, 641 |
| FspBI | CTAG | 4 cut(s) | 473, 768, 824, 901 |
| GluI | GCNGC | 2 cut(s) | 5, 641 |
| GsuI | CTGGAG | 3 cut(s) | 157, 221, 347 |
| HaeIII | GGCC | 2 cut(s) | 126, 195 |
| HapII | CCGG | 3 cut(s) | 59, 775, 893 |
| Hin1II | CATG | 6 cut(s) | 356, 545, 587, 637, 647, 751 |
| HindIII | AAGCTT | 1 cut(s) | 257 |
| HinfI | GANTC | 4 cut(s) | 55, 299, 329, 518 |
| HpaII | CCGG | 3 cut(s) | 59, 775, 893 |
| Hpy166II | GTNNAC | 2 cut(s) | 598, 655 |
| Hpy188I | TCNGA | 5 cut(s) | 304, 385, 400, 430, 729 |
| Hpy188III | TCNNGA | 3 cut(s) | 59, 136, 326 |
| Hpy8I | GTNNAC | 2 cut(s) | 598, 655 |
| HpyAV | CCTTC | 1 cut(s) | 489 |
| HpyCH4III | ACNGT | 2 cut(s) | 100, 659 |
| HpyCH4IV | ACGT | 1 cut(s) | 860 |
| HpyCH4V | TGCA | 5 cut(s) | 206, 281, 352, 541, 640 |
| HpyF3I | CTNAG | 5 cut(s) | 150, 295, 492, 525, 802 |
| HpySE526I | ACGT | 1 cut(s) | 860 |
| Hsp92II | CATG | 6 cut(s) | 356, 545, 587, 637, 647, 751 |
| Kpn2I | TCCGGA | 1 cut(s) | 58 |
| Ksp22I | TGATCA | 1 cut(s) | 247 |
| Kzo9I | GATC | 3 cut(s) | 43, 221, 247 |
| LmnI | GCTCC | 1 cut(s) | 635 |
| LpnPI | CCDG | 7 cut(s) | 72, 108, 121, 251, 311, 788, 795 |
| Lsp1109I | GCAGC | 1 cut(s) | 652 |
| LweI | GCATC | 3 cut(s) | 166, 295, 661 |
| MaeI | CTAG | 4 cut(s) | 473, 768, 824, 901 |
| MaeII | ACGT | 1 cut(s) | 860 |
| MaeIII | GTNAC | 3 cut(s) | 143, 374, 410 |
| MalI | GATC | 3 cut(s) | 45, 223, 249 |
| MboI | GATC | 3 cut(s) | 43, 221, 247 |
| MboII | GAAGA | 4 cut(s) | 417, 515, 575, 581 |
| MfeI | CAATTG | 1 cut(s) | 207 |
| MluCI | AATT | 8 cut(s) | 78, 207, 269, 465, 551, 587, 723, 742 |
| MlyI | GAGTC | 1 cut(s) | 338 |
| MmeI | TCCRAC | 1 cut(s) | 591 |
| MroI | TCCGGA | 1 cut(s) | 58 |
| MseI | TTAA | 2 cut(s) | 83, 785 |
| MspA1I | CMGCKG | 1 cut(s) | 241 |
| MspI | CCGG | 3 cut(s) | 59, 775, 893 |
| MspR9I | CCNGG | 1 cut(s) | 776 |
| MunI | CAATTG | 1 cut(s) | 207 |
| Mva1269I | GAATGC | 1 cut(s) | 407 |
| NciI | CCSGG | 1 cut(s) | 776 |
| NcoI | CCATGG | 1 cut(s) | 633 |
| NdeII | GATC | 3 cut(s) | 43, 221, 247 |
| NlaIII | CATG | 6 cut(s) | 356, 545, 587, 637, 647, 751 |
| NlaIV | GGNNCC | 1 cut(s) | 631 |
| NmuCI | GTSAC | 1 cut(s) | 410 |
| NspI | RCATGY | 3 cut(s) | 356, 545, 647 |
| NspV | TTCGAA | 1 cut(s) | 547 |
| PaeR7I | CTCGAG | 1 cut(s) | 406 |
| PctI | GAATGC | 1 cut(s) | 407 |
| PfeI | GAWTC | 3 cut(s) | 55, 299, 518 |
| PinAI | ACCGGT | 1 cut(s) | 892 |
| PkrI | GCNGC | 2 cut(s) | 6, 642 |
| PleI | GAGTC | 1 cut(s) | 337 |
| PpsI | GAGTC | 1 cut(s) | 337 |
| PshBI | ATTAAT | 1 cut(s) | 785 |
| PsiI | TTATAA | 1 cut(s) | 558 |
| PspN4I | GGNNCC | 1 cut(s) | 631 |
| PspXI | VCTCGAGB | 1 cut(s) | 406 |
| PvuII | CAGCTG | 1 cut(s) | 241 |
| RsaI | GTAC | 3 cut(s) | 97, 102, 752 |
| RsaNI | GTAC | 3 cut(s) | 96, 101, 751 |
| SaqAI | TTAA | 2 cut(s) | 83, 785 |
| SatI | GCNGC | 2 cut(s) | 5, 641 |
| Sau3AI | GATC | 3 cut(s) | 43, 221, 247 |
| SchI | GAGTC | 1 cut(s) | 338 |
| ScrFI | CCNGG | 1 cut(s) | 776 |
| SfaNI | GCATC | 3 cut(s) | 166, 295, 661 |
| Sfr274I | CTCGAG | 1 cut(s) | 406 |
| SfuI | TTCGAA | 1 cut(s) | 547 |
| SlaI | CTCGAG | 1 cut(s) | 406 |
| SmlI | CTYRAG | 1 cut(s) | 406 |
| SmoI | CTYRAG | 1 cut(s) | 406 |
| Sse9I | AATT | 8 cut(s) | 78, 207, 269, 465, 551, 587, 723, 742 |
| SsiI | CCGC | 1 cut(s) | 4 |
| SspI | AATATT | 1 cut(s) | 848 |
| SspMI | CTAG | 4 cut(s) | 473, 768, 824, 901 |
| StyD4I | CCNGG | 1 cut(s) | 774 |
| StyI | CCWWGG | 1 cut(s) | 633 |
| TaaI | ACNGT | 2 cut(s) | 100, 659 |
| TaiI | ACGT | 1 cut(s) | 863 |
| TaqI | TCGA | 2 cut(s) | 407, 547 |
| TasI | AATT | 8 cut(s) | 78, 207, 269, 465, 551, 587, 723, 742 |
| TatI | WGTACW | 1 cut(s) | 750 |
| TauI | GCSGC | 1 cut(s) | 7 |
| TfiI | GAWTC | 3 cut(s) | 55, 299, 518 |
| Tru1I | TTAA | 2 cut(s) | 83, 785 |
| Tru9I | TTAA | 2 cut(s) | 83, 785 |
| TscAI | CASTG | 1 cut(s) | 154 |
| TseFI | GTSAC | 1 cut(s) | 410 |
| TseI | GCWGC | 1 cut(s) | 640 |
| Tsp45I | GTSAC | 1 cut(s) | 410 |
| TspDTI | ATGAA | 9 cut(s) | 176, 516, 576, 582, 600, 670, 690, 702, 853 |
| TspGWI | ACGGA | 1 cut(s) | 630 |
| TspRI | CASTG | 1 cut(s) | 154 |
| VspI | ATTAAT | 1 cut(s) | 785 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 483 |
| XapI | RAATTY | 4 cut(s) | 551, 587, 723, 742 |
| XceI | RCATGY | 3 cut(s) | 356, 545, 647 |
| XhoI | CTCGAG | 1 cut(s) | 406 |
| XspI | CTAG | 4 cut(s) | 473, 768, 824, 901 |