Rroxscaffold_2G00149010

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000064
Physical Location & Seq
Reverse (-)
86627004 .. 86628351
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149010.1

Sequence Viewer

Length: 1185 bp
ATGGCTGCTACCTTCCGCAAACAACTATTGGCAGACTGGTCTAGTCTTCCTCATGTCATTCTCTCAGAGATTCTGGGTAAGTTGTTGGAACCAACTGACCATGTTCGTTTTGCTGCCGTTTGCAAGGAATGGCATGCTCTCGCAACAGACTATAACCACACAACCCAGCGTTGTTTTAAGATCTACGGCGAACATCAACTCAGTACAACTCTAGTGAACGTGTTCAAAAGAGAAGTGACGCCCATTCGTCTACCTCCATTGTATTTCAATGCCGAGAGTCATTGGTTCATGGTTTTCTCCCCAAAGGTTATCTTATCCGCTGATCCAATTGCGAATCCAGACAATTATGTGGTTGTGGCAGTTTATGATAGGGTTTCAAGGATCGCTTTCATAAAAGGAGGACAAACAATCTGGACTTACCTTGAGGTCTACTCCTTCTTTACTGATGCTATATTTTATAAAAGAAAAGTCTATGCGGTCGGACAATGGGGACGGATTAAATCGTTTGATGTTAATAGCAAGCCAGTAGAAGAAAGTTTACTTGCACATGGACGATATCGGTTTTCATGTCATGCGCATAAGGGGTATCTTGTGGAAACAACTAAAGGAGACTTGTTGCATGTTCGAAGATTTTTGAAACAGAAGGATGTACCACGAGATTACTATTTCCATTATGAGCATAATGAGTTCTGGACCGAAAGCTTCAAGGTTTACAAGGTGGTGTTTGATGAGAGGGATGAATCCATTGTGGAGCATGTTGAGTTAAAGAGTATTGGAGATGAGGCTTTGTTTGTTGGTGACAATCACTCGATGTGTGTTGTGGCTTCAAACTTTCCTGGGTGCCAACCAAATTCCATATACTACACGGATGATTTGATAGAACTTGGAGAAGTGCGATTAGTTGATGATGGATCAATTGATATGGATGACATCTTCAGTTTAATCAAACTAAAGGATGAAACCATCTCTAATGGACCAAGTGATATGGGCATCTTCCATTTAGAGGATGAAAGCATTACGCAACATTATTCTCTAAATCCTTGGCACAGAAACATCCCACCTGCTATCTGGATCATGCCGTTGTTTAATAGACTCGGCGAATTTCCAGTTCATTTCACAATCTGGAGCCATGGTGTATCTTCTCAGTCTGTTGTAACTTTCTATATTATTCCTGTACAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

394

Amino Acids

45.67

Weight (kDa)

5.87

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 13 - 50 1.1e-08 F-box-like
Beta-prop_KIB1-4 PF03478 33 - 302 1.1e-42 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AarI CACCTGC 1 cut(s) 1069
Acc16I TGCGCA 1 cut(s) 576
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 840
AccI GTMKAC 2 cut(s) 250, 429
AciI CCGC 3 cut(s) 16, 318, 476
AclWI GGATC 4 cut(s) 317, 389, 919, 1079
AcsI RAATTY 2 cut(s) 850, 1100
AcuI CTGAAG 1 cut(s) 919
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 3 cut(s) 205, 651, 1176
AfiI CCNNNNNNNGG 1 cut(s) 1003
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 6 cut(s) 226, 268, 378, 637, 706, 828
AjnI CCWGG 1 cut(s) 835
AluBI AGCT 1 cut(s) 702
AluI AGCT 1 cut(s) 702
Alw26I GTCTC 1 cut(s) 603
AlwI GGATC 4 cut(s) 317, 389, 919, 1079
ApeKI GCWGC 2 cut(s) 5, 113
ApoI RAATTY 2 cut(s) 850, 1100
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 2 cut(s) 693, 974
AsuHPI GGTGA 1 cut(s) 809
AsuII TTCGAA 1 cut(s) 625
AvaII GGWCC 2 cut(s) 693, 974
BanI GGYRCC 1 cut(s) 840
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BauI CACGAG 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 100
BccI CCATC 2 cut(s) 902, 971
BceAI ACGGC 3 cut(s) 101, 202, 1063
BciT130I CCWGG 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 2 cut(s) 42, 212
BfuAI ACCTGC 1 cut(s) 1069
BglII AGATCT 1 cut(s) 180
BisI GCNGC 2 cut(s) 6, 114
BlsI GCNGC 2 cut(s) 7, 115
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 2 cut(s) 693, 974
BmgT120I GGNCC 2 cut(s) 693, 974
BmiI GGNNCC 3 cut(s) 90, 842, 1127
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 2 cut(s) 436, 999
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BpmI CTGGAG 1 cut(s) 1144
Bpu14I TTCGAA 1 cut(s) 625
BpuEI CTTGAG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 836, 1040, 1129
BsaXI ACNNNNNCTCC 2 cut(s) 1117, 1147
Bsc4I CCNNNNNNNGG 1 cut(s) 1003
Bse1I ACTGG 3 cut(s) 41, 524, 1106
BseBI CCWGG 1 cut(s) 837
BseDI CCNNGG 3 cut(s) 836, 1040, 1129
BseGI GGATG 7 cut(s) 652, 742, 874, 931, 961, 1012, 1053
BseLI CCNNNNNNNGG 1 cut(s) 1003
BseMII CTCAG 3 cut(s) 78, 214, 1157
BseNI ACTGG 3 cut(s) 41, 524, 1106
BseXI GCAGC 1 cut(s) 100
BseYI CCCAGC 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 480
BshNI GGYRCC 1 cut(s) 840
BsiEI CGRYCG 1 cut(s) 480
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 1003
BsmAI GTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 504
Bsp119I TTCGAA 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 1174
Bsp143I GATC 5 cut(s) 180, 322, 381, 911, 1071
Bsp19I CCATGG 1 cut(s) 1129
BspACI CCGC 3 cut(s) 16, 318, 476
BspCNI CTCAG 3 cut(s) 77, 213, 1156
BspLI GGNNCC 3 cut(s) 90, 842, 1127
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 4 cut(s) 317, 389, 919, 1079
BspT104I TTCGAA 1 cut(s) 625
BspT107I GGYRCC 1 cut(s) 840
BsrGI TGTACA 1 cut(s) 1174
BsrI ACTGG 3 cut(s) 41, 524, 1106
BssECI CCNNGG 3 cut(s) 836, 1040, 1129
BssMI GATC 5 cut(s) 180, 322, 381, 911, 1071
BssNI GRCGYC 1 cut(s) 239
BssSI CACGAG 1 cut(s) 654
BssT1I CCWWGG 2 cut(s) 1040, 1129
Bst2BI CACGAG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 837
BstACI GRCGYC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 1174
BstBI TTCGAA 1 cut(s) 625
BstC8I GCNNGC 2 cut(s) 135, 521
BstDEI CTNAG 3 cut(s) 64, 200, 1143
BstDSI CCRYGG 1 cut(s) 1129
BstF5I GGATG 7 cut(s) 652, 742, 874, 931, 961, 1012, 1053
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 5 cut(s) 183, 325, 384, 914, 1074
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 5 cut(s) 180, 322, 381, 911, 1071
BstMCI CGRYCG 1 cut(s) 480
BstNI CCWGG 1 cut(s) 837
BstNSI RCATGY 3 cut(s) 137, 623, 758
BstSCI CCNGG 1 cut(s) 835
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 1129
BtsCI GGATG 7 cut(s) 652, 742, 874, 931, 961, 1012, 1053
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 2 cut(s) 135, 521
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 693, 974
CseI GACGC 1 cut(s) 247
Csp6I GTAC 3 cut(s) 204, 650, 1175
CviJI RGCY 6 cut(s) 5, 523, 702, 785, 824, 1128
CviKI_1 RGCY 6 cut(s) 5, 523, 702, 785, 824, 1128
CviQI GTAC 3 cut(s) 204, 650, 1175
DdeI CTNAG 3 cut(s) 64, 200, 1143
DpnI GATC 5 cut(s) 182, 324, 383, 913, 1073
DpnII GATC 5 cut(s) 180, 322, 381, 911, 1071
Eco130I CCWWGG 2 cut(s) 1040, 1129
Eco32I GATATC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 693, 974
Eco57I CTGAAG 1 cut(s) 919
EcoRII CCWGG 1 cut(s) 835
EcoRV GATATC 1 cut(s) 557
EcoT14I CCWWGG 2 cut(s) 1040, 1129
ErhI CCWWGG 2 cut(s) 1040, 1129
FalI AAGNNNNNCTT 4 cut(s) 296, 328, 370, 402
FaqI GGGAC 1 cut(s) 504
FblI GTMKAC 2 cut(s) 250, 429
Fnu4HI GCNGC 2 cut(s) 6, 114
FokI GGATG 7 cut(s) 659, 749, 881, 938, 968, 1019, 1040
Fsp4HI GCNGC 2 cut(s) 6, 114
FspAI RTGCGCAY 1 cut(s) 576
FspBI CTAG 2 cut(s) 42, 212
FspI TGCGCA 1 cut(s) 576
GlaI GCGC 1 cut(s) 576
GluI GCNGC 2 cut(s) 6, 114
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 1144
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 239
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 5 cut(s) 70, 277, 334, 740, 1092
HphI GGTGA 1 cut(s) 809
Hpy166II GTNNAC 5 cut(s) 217, 251, 430, 539, 712
Hpy188I TCNGA 2 cut(s) 67, 482
Hpy188III TCNNGA 5 cut(s) 338, 412, 691, 1069, 1123
Hpy8I GTNNAC 5 cut(s) 217, 251, 430, 539, 712
HpyAV CCTTC 3 cut(s) 22, 445, 637
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 123, 545, 619
HpyF3I CTNAG 3 cut(s) 64, 200, 1143
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 239
HspAI GCGC 1 cut(s) 575
Kzo9I GATC 5 cut(s) 180, 322, 381, 911, 1071
LmnI GCTCC 2 cut(s) 751, 1125
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 2 cut(s) 436, 999
MaeI CTAG 2 cut(s) 42, 212
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 3 cut(s) 235, 797, 1153
MalI GATC 5 cut(s) 182, 324, 383, 913, 1073
MboI GATC 5 cut(s) 180, 322, 381, 911, 1071
MboII GAAGA 6 cut(s) 38, 542, 639, 925, 985, 1131
MfeI CAATTG 2 cut(s) 327, 915
MflI RGATCY 1 cut(s) 180
MluCI AATT 5 cut(s) 327, 343, 850, 915, 1100
MlyI GAGTC 2 cut(s) 286, 1086
MmeI TCCRAC 2 cut(s) 66, 460
MnlI CCTC 7 cut(s) 60, 264, 392, 418, 726, 775, 997
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 6 cut(s) 177, 498, 513, 764, 941, 1086
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 837
MunI CAATTG 2 cut(s) 327, 915
MvaI CCWGG 1 cut(s) 837
NcoI CCATGG 1 cut(s) 1129
NdeII GATC 5 cut(s) 180, 322, 381, 911, 1071
NlaIV GGNNCC 3 cut(s) 90, 842, 1127
NmeAIII GCCGAG 2 cut(s) 298, 1074
NmuCI GTSAC 2 cut(s) 235, 797
NsbI TGCGCA 1 cut(s) 576
NspI RCATGY 3 cut(s) 137, 623, 758
NspV TTCGAA 1 cut(s) 625
PaeI GCATGC 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 3 cut(s) 70, 334, 740
PkrI GCNGC 2 cut(s) 7, 115
PleI GAGTC 2 cut(s) 285, 1086
PpsI GAGTC 2 cut(s) 285, 1086
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 1 cut(s) 835
PspN4I GGNNCC 3 cut(s) 90, 842, 1127
PspPI GGNCC 2 cut(s) 693, 974
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 3 cut(s) 205, 651, 1176
RsaNI GTAC 3 cut(s) 204, 650, 1175
SaqAI TTAA 6 cut(s) 177, 498, 513, 764, 941, 1086
SatI GCNGC 2 cut(s) 6, 114
Sau3AI GATC 5 cut(s) 180, 322, 381, 911, 1071
Sau96I GGNCC 2 cut(s) 693, 974
SchI GAGTC 2 cut(s) 286, 1086
ScrFI CCNGG 1 cut(s) 837
SfaNI GCATC 2 cut(s) 436, 999
SfuI TTCGAA 1 cut(s) 625
SinI GGWCC 2 cut(s) 693, 974
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SphI GCATGC 1 cut(s) 137
Sse9I AATT 5 cut(s) 327, 343, 850, 915, 1100
SsiI CCGC 3 cut(s) 16, 318, 476
SspMI CTAG 2 cut(s) 42, 212
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 2 cut(s) 1040, 1129
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 625, 809
TaqII GACCGA 1 cut(s) 710
TasI AATT 5 cut(s) 327, 343, 850, 915, 1100
TatI WGTACW 2 cut(s) 203, 1174
TfiI GAWTC 3 cut(s) 70, 334, 740
Tru1I TTAA 6 cut(s) 177, 498, 513, 764, 941, 1086
Tru9I TTAA 6 cut(s) 177, 498, 513, 764, 941, 1086
TseFI GTSAC 2 cut(s) 235, 797
TseI GCWGC 2 cut(s) 5, 113
Tsp45I GTSAC 2 cut(s) 235, 797
TspDTI ATGAA 7 cut(s) 277, 379, 555, 753, 972, 1023, 1100
TspGWI ACGGA 2 cut(s) 508, 881
VpaK11BI GGWCC 2 cut(s) 693, 974
XapI RAATTY 2 cut(s) 850, 1100
XceI RCATGY 3 cut(s) 137, 623, 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmiI GTMKAC 2 cut(s) 250, 429
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 2 cut(s) 42, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.