Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 770, 1020 |
| AccII | CGCG | 2 cut(s) | 408, 798 |
| AclI | AACGTT | 1 cut(s) | 62 |
| AclWI | GGATC | 1 cut(s) | 309 |
| AcsI | RAATTY | 6 cut(s) | 19, 229, 361, 611, 809, 824 |
| AcuI | CTGAAG | 1 cut(s) | 155 |
| AfaI | GTAC | 3 cut(s) | 190, 203, 512 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1104 |
| AgsI | TTSAA | 9 cut(s) | 88, 273, 420, 442, 587, 670, 814, 921, 991 |
| AjiI | CACGTC | 1 cut(s) | 140 |
| AjnI | CCWGG | 2 cut(s) | 315, 535 |
| AluBI | AGCT | 3 cut(s) | 446, 573, 579 |
| AluI | AGCT | 3 cut(s) | 446, 573, 579 |
| Alw26I | GTCTC | 2 cut(s) | 49, 848 |
| AlwI | GGATC | 1 cut(s) | 309 |
| ApeKI | GCWGC | 1 cut(s) | 875 |
| ApoI | RAATTY | 6 cut(s) | 19, 229, 361, 611, 809, 824 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 847, 879, 1086, 1118 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 594 |
| AspLEI | GCGC | 1 cut(s) | 410 |
| AspS9I | GGNCC | 2 cut(s) | 223, 1001 |
| AsuHPI | GGTGA | 2 cut(s) | 881, 902 |
| AsuII | TTCGAA | 1 cut(s) | 822 |
| AvaII | GGWCC | 2 cut(s) | 223, 1001 |
| BauI | CACGAG | 1 cut(s) | 728 |
| BbsI | GAAGAC | 1 cut(s) | 222 |
| BbvI | GCAGC | 1 cut(s) | 862 |
| BciT130I | CCWGG | 2 cut(s) | 317, 537 |
| BclI | TGATCA | 3 cut(s) | 565, 661, 964 |
| BcoDI | GTCTC | 2 cut(s) | 49, 848 |
| BfmI | CTRYAG | 1 cut(s) | 114 |
| BisI | GCNGC | 1 cut(s) | 876 |
| BlsI | GCNGC | 1 cut(s) | 877 |
| BmcAI | AGTACT | 1 cut(s) | 512 |
| Bme1390I | CCNGG | 2 cut(s) | 317, 537 |
| Bme18I | GGWCC | 2 cut(s) | 223, 1001 |
| BmgBI | CACGTC | 1 cut(s) | 140 |
| BmgT120I | GGNCC | 2 cut(s) | 223, 1001 |
| BmiI | GGNNCC | 1 cut(s) | 1002 |
| BmrFI | CCNGG | 2 cut(s) | 317, 537 |
| BmsI | GCATC | 3 cut(s) | 110, 862, 1054 |
| BpiI | GAAGAC | 1 cut(s) | 222 |
| BpmI | CTGGAG | 1 cut(s) | 595 |
| Bpu14I | TTCGAA | 1 cut(s) | 822 |
| BsaBI | GATNNNNATC | 3 cut(s) | 300, 937, 969 |
| BsaJI | CCNNGG | 2 cut(s) | 315, 995 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1104 |
| Bse1I | ACTGG | 1 cut(s) | 851 |
| Bse3DI | GCAATG | 2 cut(s) | 682, 1041 |
| Bse8I | GATNNNNATC | 3 cut(s) | 300, 937, 969 |
| BseBI | CCWGG | 2 cut(s) | 317, 537 |
| BseDI | CCNNGG | 2 cut(s) | 315, 995 |
| BseGI | GGATG | 1 cut(s) | 938 |
| BseJI | GATNNNNATC | 3 cut(s) | 300, 937, 969 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1104 |
| BseMI | GCAATG | 2 cut(s) | 682, 1041 |
| BseMII | CTCAG | 2 cut(s) | 41, 705 |
| BseNI | ACTGG | 1 cut(s) | 851 |
| BseRI | GAGGAG | 1 cut(s) | 521 |
| BseXI | GCAGC | 1 cut(s) | 862 |
| Bsh1236I | CGCG | 2 cut(s) | 408, 798 |
| BslFI | GGGAC | 2 cut(s) | 794, 1014 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1104 |
| BsmAI | GTCTC | 2 cut(s) | 49, 848 |
| BsmFI | GGGAC | 2 cut(s) | 794, 1014 |
| BsmI | GAATGC | 1 cut(s) | 109 |
| Bsp119I | TTCGAA | 1 cut(s) | 822 |
| Bsp1407I | TGTACA | 1 cut(s) | 201 |
| Bsp143I | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| BspCNI | CTCAG | 2 cut(s) | 40, 706 |
| BspFNI | CGCG | 2 cut(s) | 408, 798 |
| BspLI | GGNNCC | 1 cut(s) | 1002 |
| BspPI | GGATC | 1 cut(s) | 309 |
| BspT104I | TTCGAA | 1 cut(s) | 822 |
| BsrDI | GCAATG | 2 cut(s) | 682, 1041 |
| BsrGI | TGTACA | 1 cut(s) | 201 |
| BsrI | ACTGG | 1 cut(s) | 851 |
| BssECI | CCNNGG | 2 cut(s) | 315, 995 |
| BssMI | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| BssNAI | GTATAC | 2 cut(s) | 771, 1021 |
| BssSI | CACGAG | 1 cut(s) | 728 |
| BssT1I | CCWWGG | 1 cut(s) | 995 |
| Bst1107I | GTATAC | 2 cut(s) | 771, 1021 |
| Bst2BI | CACGAG | 1 cut(s) | 728 |
| Bst2UI | CCWGG | 2 cut(s) | 317, 537 |
| Bst4CI | ACNGT | 2 cut(s) | 515, 656 |
| BstAUI | TGTACA | 1 cut(s) | 201 |
| BstBI | TTCGAA | 1 cut(s) | 822 |
| BstDEI | CTNAG | 3 cut(s) | 27, 144, 714 |
| BstF5I | GGATG | 1 cut(s) | 938 |
| BstFNI | CGCG | 2 cut(s) | 408, 798 |
| BstHHI | GCGC | 1 cut(s) | 410 |
| BstKTI | GATC | 5 cut(s) | 304, 369, 568, 664, 967 |
| BstMAI | GTCTC | 2 cut(s) | 49, 848 |
| BstMBI | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| BstNI | CCWGG | 2 cut(s) | 317, 537 |
| BstSCI | CCNGG | 2 cut(s) | 315, 535 |
| BstSFI | CTRYAG | 1 cut(s) | 114 |
| BstUI | CGCG | 2 cut(s) | 408, 798 |
| BstV1I | GCAGC | 1 cut(s) | 862 |
| BstV2I | GAAGAC | 1 cut(s) | 222 |
| BstX2I | RGATCY | 1 cut(s) | 301 |
| BstYI | RGATCY | 1 cut(s) | 301 |
| BstZ17I | GTATAC | 2 cut(s) | 771, 1021 |
| BtrI | CACGTC | 1 cut(s) | 140 |
| BtsCI | GGATG | 1 cut(s) | 938 |
| CfoI | GCGC | 1 cut(s) | 410 |
| Cfr13I | GGNCC | 2 cut(s) | 223, 1001 |
| Csp6I | GTAC | 3 cut(s) | 189, 202, 511 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 891, 926 |
| CviAII | CATG | 7 cut(s) | 105, 192, 411, 552, 777, 962, 1075 |
| CviJI | RGCY | 9 cut(s) | 5, 446, 524, 573, 579, 692, 734, 917, 994 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 446, 524, 573, 579, 692, 734, 917, 994 |
| CviQI | GTAC | 3 cut(s) | 189, 202, 511 |
| DdeI | CTNAG | 3 cut(s) | 27, 144, 714 |
| DpnI | GATC | 5 cut(s) | 303, 368, 567, 663, 966 |
| DpnII | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| DraI | TTTAAA | 1 cut(s) | 738 |
| Eco130I | CCWWGG | 1 cut(s) | 995 |
| Eco32I | GATATC | 1 cut(s) | 1012 |
| Eco47I | GGWCC | 2 cut(s) | 223, 1001 |
| Eco57I | CTGAAG | 1 cut(s) | 155 |
| EcoO109I | RGGNCCY | 2 cut(s) | 223, 1001 |
| EcoRI | GAATTC | 2 cut(s) | 809, 824 |
| EcoRII | CCWGG | 2 cut(s) | 315, 535 |
| EcoRV | GATATC | 1 cut(s) | 1012 |
| EcoT14I | CCWWGG | 1 cut(s) | 995 |
| ErhI | CCWWGG | 1 cut(s) | 995 |
| FaeI | CATG | 7 cut(s) | 108, 195, 414, 555, 780, 965, 1078 |
| FaqI | GGGAC | 2 cut(s) | 794, 1014 |
| FatI | CATG | 7 cut(s) | 104, 191, 410, 551, 776, 961, 1074 |
| FbaI | TGATCA | 3 cut(s) | 565, 661, 964 |
| FblI | GTMKAC | 2 cut(s) | 770, 1020 |
| Fnu4HI | GCNGC | 1 cut(s) | 876 |
| FokI | GGATG | 1 cut(s) | 945 |
| Fsp4HI | GCNGC | 1 cut(s) | 876 |
| GlaI | GCGC | 1 cut(s) | 409 |
| GluI | GCNGC | 1 cut(s) | 876 |
| GsuI | CTGGAG | 1 cut(s) | 595 |
| HhaI | GCGC | 1 cut(s) | 410 |
| Hin1II | CATG | 7 cut(s) | 108, 195, 414, 555, 780, 965, 1078 |
| Hin6I | GCGC | 1 cut(s) | 408 |
| HinP1I | GCGC | 1 cut(s) | 408 |
| HinfI | GANTC | 7 cut(s) | 31, 41, 84, 403, 683, 788, 1090 |
| HphI | GGTGA | 2 cut(s) | 881, 902 |
| Hpy166II | GTNNAC | 2 cut(s) | 771, 1021 |
| Hpy188I | TCNGA | 6 cut(s) | 10, 30, 73, 174, 715, 1038 |
| Hpy188III | TCNNGA | 2 cut(s) | 247, 1057 |
| Hpy8I | GTNNAC | 2 cut(s) | 771, 1021 |
| Hpy99I | CGWCG | 2 cut(s) | 141, 212 |
| HpyAV | CCTTC | 3 cut(s) | 349, 386, 682 |
| HpyCH4III | ACNGT | 2 cut(s) | 515, 656 |
| HpyCH4IV | ACGT | 4 cut(s) | 62, 139, 207, 710 |
| HpyCH4V | TGCA | 6 cut(s) | 101, 235, 476, 675, 948, 1046 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 144, 714 |
| HpySE526I | ACGT | 4 cut(s) | 62, 139, 207, 710 |
| Hsp92II | CATG | 7 cut(s) | 108, 195, 414, 555, 780, 965, 1078 |
| HspAI | GCGC | 1 cut(s) | 408 |
| Ksp22I | TGATCA | 3 cut(s) | 565, 661, 964 |
| Kzo9I | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| LmnI | GCTCC | 2 cut(s) | 576, 1038 |
| LpnPI | CCDG | 7 cut(s) | 190, 302, 329, 522, 549, 559, 832 |
| Lsp1109I | GCAGC | 1 cut(s) | 862 |
| LweI | GCATC | 3 cut(s) | 110, 862, 1054 |
| MaeII | ACGT | 4 cut(s) | 62, 139, 207, 710 |
| MaeIII | GTNAC | 3 cut(s) | 63, 310, 842 |
| MalI | GATC | 5 cut(s) | 303, 368, 567, 663, 966 |
| MboI | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| MboII | GAAGA | 4 cut(s) | 227, 261, 599, 730 |
| MflI | RGATCY | 1 cut(s) | 301 |
| MlyI | GAGTC | 3 cut(s) | 412, 692, 782 |
| MnlI | CCTC | 7 cut(s) | 36, 205, 339, 402, 499, 759, 910 |
| MroXI | GAANNNNTTC | 1 cut(s) | 594 |
| MseI | TTAA | 6 cut(s) | 284, 339, 470, 581, 737, 1123 |
| MslI | CAYNNNNRTG | 1 cut(s) | 491 |
| MspR9I | CCNGG | 2 cut(s) | 317, 537 |
| Mva1269I | GAATGC | 1 cut(s) | 109 |
| MvaI | CCWGG | 2 cut(s) | 317, 537 |
| MvnI | CGCG | 2 cut(s) | 408, 798 |
| NdeII | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| NlaIII | CATG | 7 cut(s) | 108, 195, 414, 555, 780, 965, 1078 |
| NlaIV | GGNNCC | 1 cut(s) | 1002 |
| NmuCI | GTSAC | 1 cut(s) | 842 |
| NspV | TTCGAA | 1 cut(s) | 822 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 707 |
| PctI | GAATGC | 1 cut(s) | 109 |
| PdmI | GAANNNNTTC | 1 cut(s) | 594 |
| PfeI | GAWTC | 4 cut(s) | 31, 41, 84, 1090 |
| PflFI | GACNNNGTC | 1 cut(s) | 221 |
| PkrI | GCNGC | 1 cut(s) | 877 |
| PleI | GAGTC | 3 cut(s) | 411, 691, 782 |
| PpsI | GAGTC | 3 cut(s) | 411, 691, 782 |
| PpuMI | RGGWCCY | 2 cut(s) | 223, 1001 |
| Psp1406I | AACGTT | 1 cut(s) | 62 |
| Psp5II | RGGWCCY | 2 cut(s) | 223, 1001 |
| Psp6I | CCWGG | 2 cut(s) | 315, 535 |
| PspGI | CCWGG | 2 cut(s) | 315, 535 |
| PspN4I | GGNNCC | 1 cut(s) | 1002 |
| PspPI | GGNCC | 2 cut(s) | 223, 1001 |
| PspPPI | RGGWCCY | 2 cut(s) | 223, 1001 |
| PsuI | RGATCY | 1 cut(s) | 301 |
| PsyI | GACNNNGTC | 1 cut(s) | 221 |
| RsaI | GTAC | 3 cut(s) | 190, 203, 512 |
| RsaNI | GTAC | 3 cut(s) | 189, 202, 511 |
| RseI | CAYNNNNRTG | 1 cut(s) | 491 |
| SaqAI | TTAA | 6 cut(s) | 284, 339, 470, 581, 737, 1123 |
| SatI | GCNGC | 1 cut(s) | 876 |
| Sau3AI | GATC | 5 cut(s) | 301, 366, 565, 661, 964 |
| Sau96I | GGNCC | 2 cut(s) | 223, 1001 |
| ScaI | AGTACT | 1 cut(s) | 512 |
| SchI | GAGTC | 3 cut(s) | 412, 692, 782 |
| ScrFI | CCNGG | 2 cut(s) | 317, 537 |
| SfaNI | GCATC | 3 cut(s) | 110, 862, 1054 |
| SfcI | CTRYAG | 1 cut(s) | 114 |
| SfuI | TTCGAA | 1 cut(s) | 822 |
| SinI | GGWCC | 2 cut(s) | 223, 1001 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 491 |
| SspI | AATATT | 2 cut(s) | 288, 468 |
| StyD4I | CCNGG | 2 cut(s) | 315, 535 |
| StyI | CCWWGG | 1 cut(s) | 995 |
| TaaI | ACNGT | 2 cut(s) | 515, 656 |
| TaiI | ACGT | 4 cut(s) | 65, 142, 210, 713 |
| TaqI | TCGA | 7 cut(s) | 136, 210, 365, 686, 822, 941, 1093 |
| TatI | WGTACW | 2 cut(s) | 201, 510 |
| TfiI | GAWTC | 4 cut(s) | 31, 41, 84, 1090 |
| Tru1I | TTAA | 6 cut(s) | 284, 339, 470, 581, 737, 1123 |
| Tru9I | TTAA | 6 cut(s) | 284, 339, 470, 581, 737, 1123 |
| TseFI | GTSAC | 1 cut(s) | 842 |
| TseI | GCWGC | 1 cut(s) | 875 |
| Tsp45I | GTSAC | 1 cut(s) | 842 |
| TspDTI | ATGAA | 2 cut(s) | 1094, 1103 |
| TspGWI | ACGGA | 1 cut(s) | 716 |
| Tth111I | GACNNNGTC | 1 cut(s) | 221 |
| VpaK11BI | GGWCC | 2 cut(s) | 223, 1001 |
| XapI | RAATTY | 6 cut(s) | 19, 229, 361, 611, 809, 824 |
| XmiI | GTMKAC | 2 cut(s) | 770, 1020 |
| XmnI | GAANNNNTTC | 1 cut(s) | 594 |
| ZrmI | AGTACT | 1 cut(s) | 512 |