Rmu_sc0005375.1_g000003

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005375.1
OG0000064
Physical Location & Seq
Forward (+)
4933 .. 13860
8928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005375.1_g000003.1.cds

Sequence Viewer

Length: 2613 bp
atggagggatctcggcgcaaagcaggggctgccggtggtgggacaacacgggacttgcaggggggctggcggtgcggcagaactgacgctggtcaccttcgacttaggcatggagaggtagtggcgaggcagtggggtgatgacgatggtgtttgccgacggtgggattggttgtctgtagtgcttggttgctgttgggggaagtggggtctgggtcgggcccctgtctctgggctttggtgtcgtttggtttatatgctcaacgtagccttggaaatagttgcttggtggttagagtttgagccctttggcctaatggtctctgtaaccgcggtttcttggggttttggttcatgtcttccccaatattgggcatctttgcctaaggaccttctttattcagttcttgagagattagtgttaccaggagactacttgcgattcagcattgtttgtacgtcatggtattctgcagcaaaggataataaagttattcgtgcaaggatgacagctccactgctcttgatttatacaggcaaaaaagattattggaaattttacgacatcatggttaacaggttccttgatttgcaattgagagttcccaataagcggctttgtgggtcttcaaaagattggttaatatttgtggataaaaatgttgcggataagaattttggagtaaccctaattaatccattctttagggtcagaggaagaagagagaaagaaaactccatcattcgccttcctccactaacccctccaggttgggaaaagtggcttagacgatgtgagcattatgtctacaaggctacaatgtcaggtgacccaatacaagatccaaacaattgcatagtttttgttatatatttcgaacaaactctcttggcttttattaaaccgggtaaagacacaacatggacttatgttgatgcaagtgtagataatactgatcgagggtgccgcttggttgaagaagttacctgccttgaaggtaaatattatgctgtcaaccattggagtgaactcatatcttttgatgttactgctgagtcatactcaaatgtaaaattggttgtaccggccggattcaaagaaaattgcataatcaagagataccttgtggaaacaaaagagaaagaactgtggatggttcaaaaatacattgagtttgacgatgaaagatatatacgcgtgacaaagaaatttagagttttcaaattgaatttcaatttgtgcaagtggattgaagaaaccaccttaggtgagtttgcgctctttttgggagataacacttcagtagttatgaagccttcaaaattttccagttgtcgaccgaatcaaatatatttcaaccatgatagtgatttgtttagccgttacggacctcatgattatggtgtttataacatcaaagataaaagtttttcacagccttacaccaaacaggctaagaacttggtgaagaagagcaatcgactaccaatttgggttgttccaagtttgcgtctacgacgccatcaagctctgaaggagattgagatcaccgcctccacaaaaaactatccaaatggtgaccaagcaacacccgacgccgccacccacatgaatcaatcccggtacaaggagacgatcccatctccggtcaaaacgcttctgcttcacgacgaggagaagccaacctcatcttcctactgccgcttctgcttgagggagcgacgccgggggggtcctattgtagtcaaagccacgaggccaccgccgcactgcaacacgaaacctagggctagtttgcagtcgggtggaccagccggctatcactttccagtcgaccttgatcggactaccactttgcagtcgaccacgctgcccagacctccgacctcgatctctttgaccttcgtcttcactgcaaactggtctgggatgcccttggcctccatccggcaagaggagtaccgtcgtcagagcttggtcgcgaagaaatcctggacctcaggccacgatggagcagcgcagcttgtcgaaaggaggccaggggtcgaacggacctgtttgcagttgtgtggagtctccggcgacgtggttgcagtgctgggatgcctccggcctcagtccggcctgattcgcgccaccatcgaggcctcagcggggctgttgcgtcgacgtccgcactttagtgaaagtgcaggctgcgacagcggctcggggcttgccggacggaggctggaggcatcccaggcagttctgagcagcgatcgaggtcggaggagatcgaggttgcaggttctgggcagcgacctgacctgtaactgcaaggttctgggcagcgctgtaacctcgtcgccggtgactccacccgactgcagatcggtccgtccgacccctggcctccgtccgacaagctgcgccgcgccatcgcagcctgagttggggtgcagcctagacgttcgcacttcagcgaaatcatctccggcgagattcccggtgcatctccaacttgcctttcctccggatcctcaacatcatcagaaacaagctcactggaccgctcccgtcgtcgatctccactcttccaaaagcatcgattggctcggaatcgcctcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

870

Amino Acids

98.17

Weight (kDa)

9.43

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1410
AatII GACGTC 1 cut(s) 2173
Acc36I ACCTGC 2 cut(s) 995, 2278
AccB1I GGYRCC 1 cut(s) 963
AccBSI CCGCTC 1 cut(s) 2555
AccI GTMKAC 6 cut(s) 807, 1336, 1513, 1833, 1862, 2167
AccII CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
AccIII TCCGGA 1 cut(s) 2515
AclWI GGATC 5 cut(s) 16, 836, 1630, 2513, 2526
AcoI YGGCCR 1 cut(s) 1086
AcsI RAATTY 5 cut(s) 554, 673, 1208, 1228, 1322
AcuI CTGAAG 3 cut(s) 1284, 1553, 2445
AcyI GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
AfaI GTAC 4 cut(s) 457, 1083, 1625, 1961
AfeI AGCGCT 1 cut(s) 2335
AflIII ACRYGT 1 cut(s) 1195
AjiI CACGTC 1 cut(s) 2086
AjnI CCWGG 6 cut(s) 424, 766, 1991, 2038, 2241, 2389
AjuI GAANNNNNNNTTGG 4 cut(s) 1675, 1707, 2493, 2525
AleI CACNNNNGTG 1 cut(s) 2181
AluBI AGCT 6 cut(s) 512, 1529, 1974, 2023, 2409, 2543
AluI AGCT 6 cut(s) 512, 1529, 1974, 2023, 2409, 2543
Alw26I GTCTC 5 cut(s) 232, 325, 423, 1625, 2080
AlwI GGATC 5 cut(s) 16, 836, 1630, 2513, 2526
AlwNI CAGNNNCTG 2 cut(s) 29, 2293
Ama87I CYCGRG 1 cut(s) 2209
Aor13HI TCCGGA 1 cut(s) 2515
Aor51HI AGCGCT 1 cut(s) 2335
ApaI GGGCCC 1 cut(s) 223
ApoI RAATTY 5 cut(s) 554, 673, 1208, 1228, 1322
ArsI GACNNNNNNTTYG 4 cut(s) 1473, 1505, 1837, 1869
AseI ATTAAT 1 cut(s) 693
AspA2I CCTAGG 1 cut(s) 1784
AspLEI GCGC 7 cut(s) 18, 1279, 2020, 2135, 2336, 2414, 2419
AsuC2I CCSGG 4 cut(s) 906, 1621, 1727, 2489
AsuHPI GGTGA 8 cut(s) 86, 149, 839, 1280, 1477, 1540, 1589, 2365
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 1 cut(s) 2209
AvaII GGWCC 8 cut(s) 388, 1388, 1733, 1808, 1995, 2052, 2377, 2550
AvrII CCTAGG 1 cut(s) 1784
AxyI CCTNAGG 3 cut(s) 384, 1264, 1999
BaeGI GKGCMC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 2518
BanI GGYRCC 1 cut(s) 963
BanII GRGCYC 2 cut(s) 223, 306
BauI CACGAG 1 cut(s) 1753
BbsI GAAGAC 3 cut(s) 350, 618, 1900
BbvCI CCTCAGC 1 cut(s) 2149
BccI CCATC 9 cut(s) 140, 746, 1147, 1530, 1648, 1952, 2003, 2147, 2428
BceAI ACGGC 1 cut(s) 1365
BcgI CGANNNNNNTGC 4 cut(s) 1852, 1886, 2072, 2106
BciT130I CCWGG 6 cut(s) 426, 768, 1993, 2040, 2243, 2391
BcnI CCSGG 4 cut(s) 906, 1621, 1727, 2489
BcoDI GTCTC 5 cut(s) 232, 325, 423, 1625, 2080
BfaI CTAG 3 cut(s) 1785, 1791, 2447
BfmI CTRYAG 3 cut(s) 177, 471, 2368
BfoI RGCGCY 1 cut(s) 2337
BfuAI ACCTGC 2 cut(s) 995, 2278
BlnI CCTAGG 1 cut(s) 1784
Bme18I GGWCC 8 cut(s) 388, 1388, 1733, 1808, 1995, 2052, 2377, 2550
BmeT110I CYCGRG 1 cut(s) 2209
BmgBI CACGTC 1 cut(s) 2086
BmiI GGNNCC 6 cut(s) 221, 222, 581, 965, 1734, 2520
BmsI GCATC 7 cut(s) 383, 925, 1920, 2093, 2246, 2503, 2595
BoxI GACNNNNGTC 2 cut(s) 90, 1904
BpiI GAAGAC 3 cut(s) 350, 618, 1900
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
BpmI CTGGAG 2 cut(s) 750, 2252
Bpu10I CCTNAGC 1 cut(s) 2149
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 428, 1732
BpuMI CCSGG 4 cut(s) 906, 1621, 1727, 2489
Bsa29I ATCGAT 1 cut(s) 2589
BsaBI GATNNNNATC 1 cut(s) 1544
BsaHI GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 8 cut(s) 270, 330, 1726, 1784, 1935, 2039, 2241, 2389
BsaWI WCCGGW 2 cut(s) 1645, 2515
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bse118I RCCGGY 4 cut(s) 32, 1084, 1814, 2350
Bse1I ACTGG 4 cut(s) 1329, 1829, 1925, 2552
Bse21I CCTNAGG 3 cut(s) 384, 1264, 1999
Bse8I GATNNNNATC 1 cut(s) 1544
BseAI TCCGGA 1 cut(s) 2515
BseBI CCWGG 6 cut(s) 426, 768, 1993, 2040, 2243, 2391
BseCI ATCGAT 1 cut(s) 2589
BseDI CCNNGG 8 cut(s) 270, 330, 1726, 1784, 1935, 2039, 2241, 2389
BseGI GGATG 6 cut(s) 510, 1158, 1935, 1944, 2108, 2237
BseJI GATNNNNATC 1 cut(s) 1544
BseMII CTCAG 6 cut(s) 1044, 2013, 2129, 2163, 2243, 2421
BseNI ACTGG 4 cut(s) 1329, 1829, 1925, 2552
BseRI GAGGAG 3 cut(s) 1688, 1970, 2287
BseSI GKGCMC 1 cut(s) 223
BseX3I CGGCCG 1 cut(s) 1086
BseYI CCCAGC 1 cut(s) 2098
BsgI GTGCAG 2 cut(s) 2211, 2461
Bsh1236I CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
Bsh1285I CGRYCG 3 cut(s) 1089, 1340, 2263
BshNI GGYRCC 1 cut(s) 963
BshVI ATCGAT 1 cut(s) 2589
BsiEI CGRYCG 3 cut(s) 1089, 1340, 2263
BsiHKCI CYCGRG 1 cut(s) 2209
BslFI GGGAC 2 cut(s) 55, 65
BsmAI GTCTC 5 cut(s) 232, 325, 423, 1625, 2080
BsmBI CGTCTC 1 cut(s) 1625
BsmFI GGGAC 2 cut(s) 55, 65
Bso31I GGTCTC 1 cut(s) 325
BsoBI CYCGRG 1 cut(s) 2209
Bsp119I TTCGAA 1 cut(s) 876
Bsp120I GGGCCC 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 223, 306
Bsp13I TCCGGA 1 cut(s) 2515
Bsp68I TCGCGA 1 cut(s) 1982
BspCNI CTCAG 6 cut(s) 1045, 2012, 2128, 2162, 2244, 2422
BspDI ATCGAT 1 cut(s) 2589
BspEI TCCGGA 1 cut(s) 2515
BspFNI CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 6 cut(s) 221, 222, 581, 965, 1734, 2520
BspMAI CTGCAG 2 cut(s) 475, 2372
BspMI ACCTGC 2 cut(s) 995, 2278
BspPI GGATC 5 cut(s) 16, 836, 1630, 2513, 2526
BspQI GCTCTTC 1 cut(s) 1466
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 963
BspTNI GGTCTC 1 cut(s) 325
BsrBI CCGCTC 1 cut(s) 2555
BsrFI RCCGGY 4 cut(s) 32, 1084, 1814, 2350
BsrI ACTGG 4 cut(s) 1329, 1829, 1925, 2552
BssAI RCCGGY 4 cut(s) 32, 1084, 1814, 2350
BssECI CCNNGG 8 cut(s) 270, 330, 1726, 1784, 1935, 2039, 2241, 2389
BssNI GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
BssSI CACGAG 1 cut(s) 1753
BssT1I CCWWGG 3 cut(s) 270, 1784, 1935
Bst2BI CACGAG 1 cut(s) 1753
Bst2UI CCWGG 6 cut(s) 426, 768, 1993, 2040, 2243, 2391
Bst4CI ACNGT 3 cut(s) 162, 1149, 1964
Bst6I CTCTTC 3 cut(s) 715, 1466, 2581
BstACI GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
BstAPI GCANNNNNTGC 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 4 cut(s) 68, 1816, 2194, 2217
BstDSI CCRYGG 1 cut(s) 330
BstEII GGTNACC 3 cut(s) 92, 827, 1577
BstF5I GGATG 6 cut(s) 510, 1158, 1935, 1944, 2108, 2237
BstFNI CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
BstH2I RGCGCY 1 cut(s) 2337
BstHHI GCGC 7 cut(s) 18, 1279, 2020, 2135, 2336, 2414, 2419
BstMAI GTCTC 5 cut(s) 232, 325, 423, 1625, 2080
BstMCI CGRYCG 3 cut(s) 1089, 1340, 2263
BstMWI GCNNNNNNNGC 9 cut(s) 29, 72, 1707, 1765, 2130, 2202, 2205, 2243, 2425
BstNI CCWGG 6 cut(s) 426, 768, 1993, 2040, 2243, 2391
BstPAI GACNNNNGTC 2 cut(s) 90, 1904
BstPI GGTNACC 3 cut(s) 92, 827, 1577
BstSFI CTRYAG 3 cut(s) 177, 471, 2368
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
BstV2I GAAGAC 3 cut(s) 350, 618, 1900
BstX2I RGATCY 3 cut(s) 8, 841, 2518
BstYI RGATCY 3 cut(s) 8, 841, 2518
BstZI CGGCCG 1 cut(s) 1086
Bsu15I ATCGAT 1 cut(s) 2589
Bsu36I CCTNAGG 3 cut(s) 384, 1264, 1999
BsuTUI ATCGAT 1 cut(s) 2589
BtgI CCRYGG 1 cut(s) 330
BtgZI GCGATG 1 cut(s) 2406
BtrI CACGTC 1 cut(s) 2086
BtsCI GGATG 6 cut(s) 510, 1158, 1935, 1944, 2108, 2237
BtsI GCAGTG 5 cut(s) 137, 515, 1768, 1911, 2100
BtsIMutI CAGTG 6 cut(s) 137, 515, 1768, 1911, 2100, 2545
BtuMI TCGCGA 1 cut(s) 1982
BveI ACCTGC 2 cut(s) 995, 2278
Cac8I GCNNGC 4 cut(s) 68, 1816, 2194, 2217
CaiI CAGNNNCTG 2 cut(s) 29, 2293
CciI TCATGA 1 cut(s) 1393
CfoI GCGC 7 cut(s) 18, 1279, 2020, 2135, 2336, 2414, 2419
Cfr10I RCCGGY 4 cut(s) 32, 1084, 1814, 2350
Cfr42I CCGCGG 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 2589
CpoI CGGWCCG 1 cut(s) 2377
CseI GACGC 6 cut(s) 95, 1499, 1527, 1604, 1731, 2153
Csp6I GTAC 4 cut(s) 456, 1082, 1624, 1960
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CspI CGGWCCG 1 cut(s) 2377
CviAII CATG 8 cut(s) 110, 354, 462, 568, 921, 1361, 1394, 1609
CviQI GTAC 4 cut(s) 456, 1082, 1624, 1960
EaeI YGGCCR 1 cut(s) 1086
EagI CGGCCG 1 cut(s) 1086
Eam1104I CTCTTC 3 cut(s) 715, 1466, 2581
EarI CTCTTC 3 cut(s) 715, 1466, 2581
EclXI CGGCCG 1 cut(s) 1086
Eco130I CCWWGG 3 cut(s) 270, 1784, 1935
Eco147I AGGCCT 1 cut(s) 2147
Eco24I GRGCYC 2 cut(s) 223, 306
Eco31I GGTCTC 1 cut(s) 325
Eco47I GGWCC 8 cut(s) 388, 1388, 1733, 1808, 1995, 2052, 2377, 2550
Eco47III AGCGCT 1 cut(s) 2335
Eco52I CGGCCG 1 cut(s) 1086
Eco57I CTGAAG 3 cut(s) 1284, 1553, 2445
Eco81I CCTNAGG 3 cut(s) 384, 1264, 1999
Eco88I CYCGRG 1 cut(s) 2209
Eco91I GGTNACC 3 cut(s) 92, 827, 1577
EcoO109I RGGNCCY 3 cut(s) 220, 388, 1733
EcoO65I GGTNACC 3 cut(s) 92, 827, 1577
EcoRII CCWGG 6 cut(s) 424, 766, 1991, 2038, 2241, 2389
EcoT14I CCWWGG 3 cut(s) 270, 1784, 1935
EcoT38I GRGCYC 2 cut(s) 223, 306
ErhI CCWWGG 3 cut(s) 270, 1784, 1935
Esp3I CGTCTC 1 cut(s) 1625
FaeI CATG 8 cut(s) 113, 357, 465, 571, 924, 1364, 1397, 1612
FaqI GGGAC 2 cut(s) 55, 65
FatI CATG 8 cut(s) 109, 353, 461, 567, 920, 1360, 1393, 1608
FauI CCCGC 1 cut(s) 2146
FblI GTMKAC 6 cut(s) 807, 1336, 1513, 1833, 1862, 2167
FokI GGATG 6 cut(s) 517, 1165, 1931, 1942, 2115, 2224
FriOI GRGCYC 2 cut(s) 223, 306
FspBI CTAG 3 cut(s) 1785, 1791, 2447
GlaI GCGC 7 cut(s) 17, 1278, 2019, 2134, 2335, 2413, 2418
GsaI CCCAGC 1 cut(s) 2102
GsuI CTGGAG 2 cut(s) 750, 2252
HaeII RGCGCY 1 cut(s) 2337
HgaI GACGC 6 cut(s) 95, 1499, 1527, 1604, 1731, 2153
HhaI GCGC 7 cut(s) 18, 1279, 2020, 2135, 2336, 2414, 2419
Hin1I GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
Hin1II CATG 8 cut(s) 113, 357, 465, 571, 924, 1364, 1397, 1612
Hin6I GCGC 7 cut(s) 16, 1277, 2018, 2133, 2334, 2412, 2417
HinP1I GCGC 7 cut(s) 16, 1277, 2018, 2133, 2334, 2412, 2417
HincII GTYRAC 6 cut(s) 574, 1015, 1337, 1834, 1863, 2168
HindII GTYRAC 6 cut(s) 574, 1015, 1337, 1834, 1863, 2168
HpaI GTTAAC 1 cut(s) 574
HphI GGTGA 8 cut(s) 86, 149, 839, 1280, 1477, 1540, 1589, 2365
Hpy188III TCNNGA 7 cut(s) 407, 523, 1114, 1394, 1667, 1981, 2516
HpyAV CCTTC 7 cut(s) 107, 401, 758, 989, 1326, 1528, 1912
HpyCH4III ACNGT 3 cut(s) 162, 1149, 1964
HpyCH4IV ACGT 5 cut(s) 264, 458, 2085, 2170, 2451
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 72, 1707, 1765, 2130, 2202, 2205, 2243, 2425
HpySE526I ACGT 5 cut(s) 264, 458, 2085, 2170, 2451
Hsp92I GRCGYC 4 cut(s) 1519, 1596, 1723, 2170
Hsp92II CATG 8 cut(s) 113, 357, 465, 571, 924, 1364, 1397, 1612
HspAI GCGC 7 cut(s) 16, 1277, 2018, 2133, 2334, 2412, 2417
Kpn2I TCCGGA 1 cut(s) 2515
KroI GCCGGC 1 cut(s) 1814
KroNI GCCGGC 1 cut(s) 1816
KspAI GTTAAC 1 cut(s) 574
KspI CCGCGG 1 cut(s) 333
LguI GCTCTTC 1 cut(s) 1466
LmnI GCTCC 4 cut(s) 517, 1717, 2012, 2560
LweI GCATC 7 cut(s) 383, 925, 1920, 2093, 2246, 2503, 2595
MaeI CTAG 3 cut(s) 1785, 1791, 2447
MaeII ACGT 5 cut(s) 264, 458, 2085, 2170, 2451
MbiI CCGCTC 1 cut(s) 2555
MfeI CAATTG 2 cut(s) 593, 850
MflI RGATCY 3 cut(s) 8, 841, 2518
MhlI GDGCHC 2 cut(s) 223, 306
MluI ACGCGT 1 cut(s) 1195
MlyI GAGTC 3 cut(s) 1064, 2082, 2350
MmeI TCCRAC 5 cut(s) 1907, 2249, 2408, 2426, 2524
MroI TCCGGA 1 cut(s) 2515
MroNI GCCGGC 1 cut(s) 1814
MseI TTAA 4 cut(s) 573, 641, 693, 900
MslI CAYNNNNRTG 5 cut(s) 1023, 1365, 1398, 1607, 2181
MspA1I CMGCKG 3 cut(s) 332, 2153, 2205
MteI GCGCNGCGC 1 cut(s) 2415
MunI CAATTG 2 cut(s) 593, 850
MvaI CCWGG 6 cut(s) 426, 768, 1993, 2040, 2243, 2391
MvnI CGCG 5 cut(s) 332, 1197, 1982, 2133, 2417
MwoI GCNNNNNNNGC 9 cut(s) 29, 72, 1707, 1765, 2130, 2202, 2205, 2243, 2425
NaeI GCCGGC 1 cut(s) 1816
NciI CCSGG 4 cut(s) 906, 1621, 1727, 2489
NgoMIV GCCGGC 1 cut(s) 1814
NlaIII CATG 8 cut(s) 113, 357, 465, 571, 924, 1364, 1397, 1612
NlaIV GGNNCC 6 cut(s) 221, 222, 581, 965, 1734, 2520
NmuCI GTSAC 5 cut(s) 92, 827, 1198, 1577, 2353
NruI TCGCGA 1 cut(s) 1982
NspV TTCGAA 1 cut(s) 876
OliI CACNNNNGTG 1 cut(s) 2181
PagI TCATGA 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 2147
PciSI GCTCTTC 1 cut(s) 1466
PcsI WCGNNNNNNNCGW 1 cut(s) 2381
PdiI GCCGGC 1 cut(s) 1816
PfeI GAWTC 7 cut(s) 441, 1092, 1342, 1612, 2128, 2484, 2601
PfoI TCCNGGA 1 cut(s) 1991
Ple19I CGATCG 1 cut(s) 2263
PleI GAGTC 3 cut(s) 1063, 2081, 2350
PpsI GAGTC 3 cut(s) 1063, 2081, 2350
PpuMI RGGWCCY 2 cut(s) 388, 1733
PshAI GACNNNNGTC 2 cut(s) 90, 1904
PshBI ATTAAT 1 cut(s) 693
PsiI TTATAA 1 cut(s) 1410
Psp5II RGGWCCY 2 cut(s) 388, 1733
Psp6I CCWGG 6 cut(s) 424, 766, 1991, 2038, 2241, 2389
PspEI GGTNACC 3 cut(s) 92, 827, 1577
PspFI CCCAGC 1 cut(s) 2098
PspGI CCWGG 6 cut(s) 424, 766, 1991, 2038, 2241, 2389
PspN4I GGNNCC 6 cut(s) 221, 222, 581, 965, 1734, 2520
PspOMI GGGCCC 1 cut(s) 219
PspPPI RGGWCCY 2 cut(s) 388, 1733
PstI CTGCAG 2 cut(s) 475, 2372
PstNI CAGNNNCTG 2 cut(s) 29, 2293
PsuI RGATCY 3 cut(s) 8, 841, 2518
PvuI CGATCG 1 cut(s) 2263
RruI TCGCGA 1 cut(s) 1982
RsaI GTAC 4 cut(s) 457, 1083, 1625, 1961
RsaNI GTAC 4 cut(s) 456, 1082, 1624, 1960
RseI CAYNNNNRTG 5 cut(s) 1023, 1365, 1398, 1607, 2181
Rsr2I CGGWCCG 1 cut(s) 2377
RsrII CGGWCCG 1 cut(s) 2377
SacII CCGCGG 1 cut(s) 333
SalI GTCGAC 4 cut(s) 1335, 1832, 1861, 2166
SapI GCTCTTC 1 cut(s) 1466
SaqAI TTAA 4 cut(s) 573, 641, 693, 900
SchI GAGTC 3 cut(s) 1064, 2082, 2350
SduI GDGCHC 2 cut(s) 223, 306
SfaNI GCATC 7 cut(s) 383, 925, 1920, 2093, 2246, 2503, 2595
SfcI CTRYAG 3 cut(s) 177, 471, 2368
Sfr303I CCGCGG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 876
SgrAI CRCCGGYG 1 cut(s) 2350
SgrBI CCGCGG 1 cut(s) 333
SgrDI CGTCGACG 1 cut(s) 2166
SinI GGWCC 8 cut(s) 388, 1388, 1733, 1808, 1995, 2052, 2377, 2550
SmiMI CAYNNNNRTG 5 cut(s) 1023, 1365, 1398, 1607, 2181
SmlI CTYRAG 2 cut(s) 407, 1711
SmoI CTYRAG 2 cut(s) 407, 1711
SseBI AGGCCT 1 cut(s) 2147
SspI AATATT 3 cut(s) 368, 645, 1004
SspMI CTAG 3 cut(s) 1785, 1791, 2447
StuI AGGCCT 1 cut(s) 2147
StyI CCWWGG 3 cut(s) 270, 1784, 1935
TaaI ACNGT 3 cut(s) 162, 1149, 1964
TaiI ACGT 5 cut(s) 267, 461, 2088, 2173, 2454
TaqII GACCGA 2 cut(s) 1354, 2365
TauI GCSGC 8 cut(s) 78, 616, 969, 1601, 1704, 1768, 2208, 2417
TfiI GAWTC 7 cut(s) 441, 1092, 1342, 1612, 2128, 2484, 2601
Tru1I TTAA 4 cut(s) 573, 641, 693, 900
Tru9I TTAA 4 cut(s) 573, 641, 693, 900
TscAI CASTG 6 cut(s) 137, 522, 1775, 1918, 2100, 2552
TseFI GTSAC 5 cut(s) 92, 827, 1198, 1577, 2353
Tsp45I GTSAC 5 cut(s) 92, 827, 1198, 1577, 2353
TspDTI ATGAA 4 cut(s) 342, 1197, 1325, 1625
TspGWI ACGGA 5 cut(s) 1401, 2065, 2239, 2369, 2387
TspRI CASTG 6 cut(s) 137, 522, 1775, 1918, 2100, 2552
VpaK11BI GGWCC 8 cut(s) 388, 1388, 1733, 1808, 1995, 2052, 2377, 2550
VspI ATTAAT 1 cut(s) 693
XapI RAATTY 5 cut(s) 554, 673, 1208, 1228, 1322
XmaJI CCTAGG 1 cut(s) 1784
XmiI GTMKAC 6 cut(s) 807, 1336, 1513, 1833, 1862, 2167
XspI CTAG 3 cut(s) 1785, 1791, 2447
ZraI GACGTC 1 cut(s) 2171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.