Rh2BG373500

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000064
Physical Location & Seq
Reverse (-)
52458102 .. 52483662
25561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG373500.1

Sequence Viewer

Length: 2199 bp
ATGAAGACATGGCTGACGGAACTAGAAATTGGGGGTGCGACGATACCACTTTTCACCATTGAAGATGACGAAGGAGCTTGGCCAGAACCGTCTTGCAAACACTGCAGAATTGCAGACTGCGAAAGCATTCATGTTTCAAAGAAACGGTTCCATATGTTAGTTCCAGTTGAGGAAGTGTGGAATGAATGCCTTAATGAGAGTGTTTTGGATAACCCACAAGGAAATTCCTTGCTGTATGTGGTGATACACATGAATGCCTATGCCCATTTGCTTTGGATTCAAAGAAGTTCTTCTGGTGTGATGTCTGGCAGTAACCTCATGGACATATGGGACTGTCTATGTAAAAATCTCGAAATAAGGGATGTTACATTGGAGGATGTTTCCAAGAAAGGGAATGTTGATCTTCGCTTGCTCTGTGGGGTGGCTTACGGAAGATCCTGGTTCGGTAGGTGGGGATACAAATTTTGGAGAGGGAGTTATGGAGTAGATGAAGTGAAGTATGAGATTGCCCTAGCTTGTCTCCGTAACCTTGACATTTCTCAGGTTGTTGAAGTGTTTCGCAAGGTGGAAGACCAATATGCTCTAGTTCATCGTCTAGAAGGAGTACTTGAACGTTACCGACGTCTCAGTCATTCCCCATTCAAGACTTTAAGTGACTTGCTTGTGTTCATTCTGACTTATCCTGCCAAATCAAGGATGGCAAAAGAGGCATGTACCATTGCAAGCATACCCCGCAAAGGTTGGACTAGGGAGCAAATAAACGAGACGTTAAAGGTACTGATCAATATACTGGATAAGAGAGGCCCTGTAAGCGGGGAAGACCTCATTGAGGCAGCTAGCTCCAAAGTAAGTCCAGGTGAGCTACTGGAATATGTGATTTCAACAATGAAGAATCTGAAGTGTGGAATGCACCAAATTGTGGCTCGTAAGGAGAATCCAATCTCAAATCTGACGGAGTATAGTCTGGAGACTGTTCCTCTCCTGACAACTGAGGCCATTGATTATTACGGTGATGCAGTTTTTGTATATGAAACTGTTGTTCTACAATACCCTGAAGTCGGCACTGAGCAATACCCGTCGGCTGAGCTGTCGGCAAAATTGGAGGACCTTTCCGATCGGCAACTACAGCCACCGGAGATTGAAGGACGGCAAGCCAACGCCGTCCACCGGCAGCAGTCGAAAAGGAACATGGCTGCTACCTTCCGCAAACAACTATTGGCAGACTGGTCTAGTCTTCCTCATGTCATTCTCTCAGACATTCTGGATAAGTTGTTGGAACCAATTGACCATGTTCGTTTTGCTGCCGTTTGCAAGGAATGGCATGCTCTCGCAACAGACTATAACCACACAACCCAGCGTTGTTTTAAGGTGCATCCCATGCTTATGATCCCTTCAGAATCCCAAGGAAAGCGAAAACTATACAGCATCTCTGAGCAAAAAGTCTACAACAACTTTGAGTTACCACAAGCTTGTAATAGCACTAAGAGGTGTTGTGGCTCGAGCAATGCTCCTGGTTGGTTGGTCGCAGTAGATCTACTAGGCGAACATCAACTCAGTACAACTCTTGTGAACGTGTTCAAAAGAGAAGTGACGCCCATTTGTCTACCTCCATTGCATTTCAATGCCCAGACTCATTGGTTCAGGGTTTTCTCCCCAAAGGTTATCTTATCAGCTGATCCAATTGCGAATCCAGACAATTATGTGGTTGTGGCAGTTTATGATAGAGTTTCAAGGTTCGCTTTCATAAAAGGAGGACAAACAGTCTGGACTTACCTTGAGGTCCGCTCCTTCTTTACTGATGCTATATTTTATAAAAGAAAAGTCTATGCTGTCGGACAATGGGGACGGATTACCTCGTTTGATGTTAATAGCAAGTCAGTAGAAGAAAATTTACTTACACATGGAGGATATCCGTTTTCATGTTATGCGCATAAGGGGTATCTTGTGGAAACAACCAAAGGAGACTTGTTGCATGTTCGAAGATTTTTGAAACAGAAGGATGTACCACGAGATCACTATTTCCATTATGAGGATAATGAGTTCTGGACTGAGAGCTTCAAGGTTTACAAGGTGGTGTTTGATGAGAGGGACGAATCCATTGTGGAGCAGGTTGAGTTAAAGAGTATTGGAGATGAGGCTTTGTTTGTTGGTGACAATCACTCGATTCAACGAAGTGAGACGATGGAACTCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

732

Amino Acids

83.89

Weight (kDa)

6.06

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 248 - 324 2.9e-08 Winged helix-turn-helix domain in plant reproductive proteins
F-box-like PF12937 409 - 446 8e-08 F-box-like
Beta-prop_KIB1-4 PF03478 473 - 723 4.8e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1812
AasI GACNNNNNNGTC 1 cut(s) 627
AatII GACGTC 1 cut(s) 625
Acc16I TGCGCA 1 cut(s) 1929
Acc36I ACCTGC 1 cut(s) 2098
AccB7I CCANNNNNTGG 1 cut(s) 919
AccBSI CCGCTC 1 cut(s) 1785
AccI GTMKAC 2 cut(s) 1443, 1603
AciI CCGC 4 cut(s) 733, 813, 1204, 1783
AclI AACGTT 1 cut(s) 613
AclWI GGATC 3 cut(s) 429, 1381, 1670
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 3 cut(s) 223, 461, 1888
AcuI CTGAAG 3 cut(s) 917, 1074, 1377
AcyI GRCGYC 2 cut(s) 622, 1592
AfaI GTAC 5 cut(s) 606, 715, 777, 1558, 2004
AfiI CCNNNNNNNGG 9 cut(s) 390, 693, 737, 812, 829, 919, 1058, 1167, 2029
AflIII ACRYGT 1 cut(s) 1572
AhdI GACNNNNNGTC 1 cut(s) 1760
AjnI CCWGG 3 cut(s) 437, 853, 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AloI GAACNNNNNNTCC 2 cut(s) 2024, 2056
AluBI AGCT 9 cut(s) 77, 515, 836, 840, 862, 1087, 1469, 1673, 2055
AluI AGCT 9 cut(s) 77, 515, 836, 840, 862, 1087, 1469, 1673, 2055
Alw26I GTCTC 6 cut(s) 524, 629, 758, 962, 1956, 2171
AlwI GGATC 3 cut(s) 429, 1381, 1670
Ama87I CYCGRG 1 cut(s) 1498
AoxI GGCC 3 cut(s) 80, 802, 993
ApeKI GCWGC 4 cut(s) 833, 1171, 1193, 1301
ApoI RAATTY 3 cut(s) 223, 461, 1888
Asp700I GAANNNNTTC 5 cut(s) 126, 146, 289, 555, 1574
AspLEI GCGC 1 cut(s) 1930
AspS9I GGNCC 3 cut(s) 803, 1105, 1780
AsuHPI GGTGA 5 cut(s) 46, 253, 869, 1022, 2162
AsuII TTCGAA 1 cut(s) 1978
AsuNHI GCTAGC 1 cut(s) 836
AvaI CYCGRG 1 cut(s) 1498
AvaII GGWCC 2 cut(s) 1105, 1780
BalI TGGCCA 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 1875, 1907
BauI CACGAG 1 cut(s) 2007
BbsI GAAGAC 4 cut(s) 11, 576, 825, 1226
BbvI GCAGC 4 cut(s) 845, 1180, 1183, 1288
BccI CCATC 2 cut(s) 691, 2176
BceAI ACGGC 3 cut(s) 1145, 1163, 1289
BciT130I CCWGG 3 cut(s) 439, 855, 1512
BciVI GTATCC 1 cut(s) 449
BclI TGATCA 1 cut(s) 780
BcoDI GTCTC 6 cut(s) 524, 629, 758, 962, 1956, 2171
BfaI CTAG 8 cut(s) 23, 512, 584, 596, 747, 837, 1230, 1538
BfmI CTRYAG 2 cut(s) 103, 1124
BfuAI ACCTGC 1 cut(s) 2098
BfuI GTATCC 1 cut(s) 449
BglII AGATCT 1 cut(s) 1531
BisI GCNGC 4 cut(s) 834, 1172, 1194, 1302
BlpI GCTNAGC 1 cut(s) 1083
BlsI GCNGC 4 cut(s) 835, 1173, 1195, 1303
BmcAI AGTACT 1 cut(s) 606
Bme1390I CCNGG 3 cut(s) 439, 855, 1512
Bme18I GGWCC 2 cut(s) 1105, 1780
BmeRI GACNNNNNGTC 1 cut(s) 1760
BmeT110I CYCGRG 1 cut(s) 1498
BmgT120I GGNCC 3 cut(s) 803, 1105, 1780
BmiI GGNNCC 2 cut(s) 149, 1278
BmrFI CCNGG 3 cut(s) 439, 855, 1512
BmsI GCATC 4 cut(s) 1003, 1381, 1434, 1789
BmtI GCTAGC 1 cut(s) 840
BpiI GAAGAC 4 cut(s) 11, 576, 825, 1226
BplI GAGNNNNNCTC 4 cut(s) 1492, 1524, 1769, 1801
BpmI CTGGAG 1 cut(s) 986
Bpu1102I GCTNAGC 1 cut(s) 1083
Bpu14I TTCGAA 1 cut(s) 1978
BpuEI CTTGAG 1 cut(s) 1796
BsaHI GRCGYC 2 cut(s) 622, 1592
BsaJI CCNNGG 1 cut(s) 1402
BsaWI WCCGGW 1 cut(s) 1132
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 9 cut(s) 390, 693, 737, 812, 829, 919, 1058, 1167, 2029
Bse118I RCCGGY 1 cut(s) 1167
Bse1I ACTGG 4 cut(s) 164, 795, 870, 1229
Bse3DI GCAATG 3 cut(s) 717, 1510, 1610
BseBI CCWGG 3 cut(s) 439, 855, 1512
BseDI CCNNGG 1 cut(s) 1402
BseGI GGATG 5 cut(s) 367, 382, 702, 1372, 2005
BseLI CCNNNNNNNGG 9 cut(s) 390, 693, 737, 812, 829, 919, 1058, 1167, 2029
BseMI GCAATG 3 cut(s) 717, 1510, 1610
BseMII CTCAG 9 cut(s) 554, 640, 981, 1056, 1074, 1266, 1422, 1567, 2040
BseNI ACTGG 4 cut(s) 164, 795, 870, 1229
BseXI GCAGC 4 cut(s) 845, 1180, 1183, 1288
BseYI CCCAGC 1 cut(s) 1353
Bsh1285I CGRYCG 1 cut(s) 1117
BshFI GGCC 3 cut(s) 82, 804, 995
BsiEI CGRYCG 1 cut(s) 1117
BsiHKCI CYCGRG 1 cut(s) 1498
BsiSI CCGG 2 cut(s) 1133, 1168
BslFI GGGAC 3 cut(s) 344, 1857, 2102
BslI CCNNNNNNNGG 9 cut(s) 390, 693, 737, 812, 829, 919, 1058, 1167, 2029
BsmAI GTCTC 6 cut(s) 524, 629, 758, 962, 1956, 2171
BsmBI CGTCTC 3 cut(s) 629, 758, 2171
BsmFI GGGAC 3 cut(s) 344, 1857, 2102
BsmI GAATGC 4 cut(s) 126, 191, 259, 912
BsnI GGCC 3 cut(s) 82, 804, 995
BsoBI CYCGRG 1 cut(s) 1498
Bsp119I TTCGAA 1 cut(s) 1978
Bsp143I GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
Bsp1720I GCTNAGC 1 cut(s) 1083
BspACI CCGC 4 cut(s) 733, 813, 1204, 1783
BspANI GGCC 3 cut(s) 82, 804, 995
BspCNI CTCAG 9 cut(s) 553, 639, 982, 1057, 1075, 1265, 1423, 1566, 2041
BspLI GGNNCC 2 cut(s) 149, 1278
BspMAI CTGCAG 1 cut(s) 107
BspMI ACCTGC 1 cut(s) 2098
BspOI GCTAGC 1 cut(s) 840
BspPI GGATC 3 cut(s) 429, 1381, 1670
BspT104I TTCGAA 1 cut(s) 1978
BsrBI CCGCTC 1 cut(s) 1785
BsrDI GCAATG 3 cut(s) 717, 1510, 1610
BsrFI RCCGGY 1 cut(s) 1167
BsrI ACTGG 4 cut(s) 164, 795, 870, 1229
BssAI RCCGGY 1 cut(s) 1167
BssECI CCNNGG 1 cut(s) 1402
BssMI GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
BssNI GRCGYC 2 cut(s) 622, 1592
BssSI CACGAG 1 cut(s) 2007
BssT1I CCWWGG 1 cut(s) 1402
Bst2BI CACGAG 1 cut(s) 2007
Bst2UI CCWGG 3 cut(s) 439, 855, 1512
Bst4CI ACNGT 7 cut(s) 90, 147, 335, 973, 1010, 1036, 1762
BstACI GRCGYC 2 cut(s) 622, 1592
BstAPI GCANNNNNTGC 2 cut(s) 102, 1378
BstBI TTCGAA 1 cut(s) 1978
BstC8I GCNNGC 5 cut(s) 410, 724, 838, 1152, 1323
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 5 cut(s) 367, 382, 702, 1372, 2005
BstHHI GCGC 1 cut(s) 1930
BstKTI GATC 8 cut(s) 403, 437, 783, 1117, 1389, 1534, 1678, 2014
BstMAI GTCTC 6 cut(s) 524, 629, 758, 962, 1956, 2171
BstMBI GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
BstMCI CGRYCG 1 cut(s) 1117
BstMWI GCNNNNNNNGC 6 cut(s) 102, 707, 732, 810, 1126, 1378
BstNI CCWGG 3 cut(s) 439, 855, 1512
BstNSI RCATGY 3 cut(s) 714, 1325, 1976
BstSCI CCNGG 3 cut(s) 437, 853, 1510
BstSFI CTRYAG 2 cut(s) 103, 1124
BstV1I GCAGC 4 cut(s) 845, 1180, 1183, 1288
BstV2I GAAGAC 4 cut(s) 11, 576, 825, 1226
BstX2I RGATCY 2 cut(s) 434, 1531
BstYI RGATCY 2 cut(s) 434, 1531
BsuI GTATCC 1 cut(s) 449
BsuRI GGCC 3 cut(s) 82, 804, 995
BtsCI GGATG 5 cut(s) 367, 382, 702, 1372, 2005
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 100, 1062
BveI ACCTGC 1 cut(s) 2098
Cac8I GCNNGC 5 cut(s) 410, 724, 838, 1152, 1323
CfoI GCGC 1 cut(s) 1930
Cfr10I RCCGGY 1 cut(s) 1167
Cfr13I GGNCC 3 cut(s) 803, 1105, 1780
CseI GACGC 1 cut(s) 1600
Csp6I GTAC 5 cut(s) 605, 714, 776, 1557, 2003
CviQI GTAC 5 cut(s) 605, 714, 776, 1557, 2003
DpnI GATC 8 cut(s) 402, 436, 782, 1116, 1388, 1533, 1677, 2013
DpnII GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
DrdI GACNNNNNNGTC 1 cut(s) 627
DriI GACNNNNNGTC 1 cut(s) 1760
DseDI GACNNNNNNGTC 1 cut(s) 627
EaeI YGGCCR 1 cut(s) 80
Eam1105I GACNNNNNGTC 1 cut(s) 1760
Eco130I CCWWGG 1 cut(s) 1402
Eco32I GATATC 1 cut(s) 1910
Eco47I GGWCC 2 cut(s) 1105, 1780
Eco57I CTGAAG 3 cut(s) 917, 1074, 1377
Eco88I CYCGRG 1 cut(s) 1498
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoO109I RGGNCCY 2 cut(s) 803, 1105
EcoRII CCWGG 3 cut(s) 437, 853, 1510
EcoRV GATATC 1 cut(s) 1910
EcoT14I CCWWGG 1 cut(s) 1402
ErhI CCWWGG 1 cut(s) 1402
Esp3I CGTCTC 3 cut(s) 629, 758, 2171
FalI AAGNNNNNCTT 8 cut(s) 274, 306, 591, 623, 1649, 1681, 1723, 1755
FaqI GGGAC 3 cut(s) 344, 1857, 2102
FauI CCCGC 2 cut(s) 740, 806
FauNDI CATATG 2 cut(s) 153, 326
FbaI TGATCA 1 cut(s) 780
FblI GTMKAC 2 cut(s) 1443, 1603
Fnu4HI GCNGC 4 cut(s) 834, 1172, 1194, 1302
FokI GGATG 5 cut(s) 374, 389, 709, 1359, 2012
Fsp4HI GCNGC 4 cut(s) 834, 1172, 1194, 1302
FspAI RTGCGCAY 1 cut(s) 1929
FspBI CTAG 8 cut(s) 23, 512, 584, 596, 747, 837, 1230, 1538
FspI TGCGCA 1 cut(s) 1929
GlaI GCGC 1 cut(s) 1929
GluI GCNGC 4 cut(s) 834, 1172, 1194, 1302
GsaI CCCAGC 1 cut(s) 1357
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 3 cut(s) 82, 804, 995
HapII CCGG 2 cut(s) 1133, 1168
HgaI GACGC 1 cut(s) 1600
HhaI GCGC 1 cut(s) 1930
Hin1I GRCGYC 2 cut(s) 622, 1592
Hin6I GCGC 1 cut(s) 1928
HinP1I GCGC 1 cut(s) 1928
HindIII AAGCTT 1 cut(s) 1467
HinfI GANTC 8 cut(s) 277, 892, 934, 1397, 1630, 1687, 2093, 2164
HpaII CCGG 2 cut(s) 1133, 1168
HphI GGTGA 5 cut(s) 46, 253, 869, 1022, 2162
Hpy166II GTNNAC 5 cut(s) 1165, 1444, 1570, 1604, 2065
Hpy188I TCNGA 8 cut(s) 675, 897, 951, 1114, 1255, 1396, 1432, 1835
Hpy188III TCNNGA 9 cut(s) 350, 596, 643, 965, 982, 1262, 1691, 1765, 2044
Hpy8I GTNNAC 5 cut(s) 1165, 1444, 1570, 1604, 2065
Hpy99I CGWCG 3 cut(s) 43, 624, 1081
HpyAV CCTTC 7 cut(s) 65, 593, 1136, 1210, 1401, 1798, 1990
HpyCH4III ACNGT 7 cut(s) 90, 147, 335, 973, 1010, 1036, 1762
HpyCH4IV ACGT 4 cut(s) 613, 622, 767, 1572
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 707, 732, 810, 1126, 1378
HpySE526I ACGT 4 cut(s) 613, 622, 767, 1572
Hsp92I GRCGYC 2 cut(s) 622, 1592
HspAI GCGC 1 cut(s) 1928
Ksp22I TGATCA 1 cut(s) 780
Kzo9I GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
LmnI GCTCC 6 cut(s) 74, 751, 845, 1513, 1790, 2104
Lsp1109I GCAGC 4 cut(s) 845, 1180, 1183, 1288
LweI GCATC 4 cut(s) 1003, 1381, 1434, 1789
MaeI CTAG 8 cut(s) 23, 512, 584, 596, 747, 837, 1230, 1538
MaeII ACGT 4 cut(s) 613, 622, 767, 1572
MaeIII GTNAC 8 cut(s) 311, 364, 524, 614, 653, 1458, 1588, 2150
MalI GATC 8 cut(s) 402, 436, 782, 1116, 1388, 1533, 1677, 2013
MbiI CCGCTC 1 cut(s) 1785
MboI GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
MfeI CAATTG 2 cut(s) 1281, 1680
MflI RGATCY 2 cut(s) 434, 1531
MlsI TGGCCA 1 cut(s) 82
MluNI TGGCCA 1 cut(s) 82
MlyI GAGTC 1 cut(s) 1624
MmeI TCCRAC 3 cut(s) 722, 1254, 1813
Mox20I TGGCCA 1 cut(s) 82
MroXI GAANNNNTTC 5 cut(s) 126, 146, 289, 555, 1574
MscI TGGCCA 1 cut(s) 82
MseI TTAA 6 cut(s) 192, 650, 770, 1365, 1866, 2117
MslI CAYNNNNRTG 3 cut(s) 252, 1382, 1620
Msp20I TGGCCA 1 cut(s) 82
MspA1I CMGCKG 1 cut(s) 1673
MspI CCGG 2 cut(s) 1133, 1168
MspR9I CCNGG 3 cut(s) 439, 855, 1512
MunI CAATTG 2 cut(s) 1281, 1680
Mva1269I GAATGC 4 cut(s) 126, 191, 259, 912
MvaI CCWGG 3 cut(s) 439, 855, 1512
MwoI GCNNNNNNNGC 6 cut(s) 102, 707, 732, 810, 1126, 1378
NdeI CATATG 2 cut(s) 153, 326
NdeII GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
NheI GCTAGC 1 cut(s) 836
NlaIV GGNNCC 2 cut(s) 149, 1278
NmuCI GTSAC 3 cut(s) 653, 1588, 2150
NsbI TGCGCA 1 cut(s) 1929
NspI RCATGY 3 cut(s) 714, 1325, 1976
NspV TTCGAA 1 cut(s) 1978
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 1 cut(s) 1498
PcsI WCGNNNNNNNCGW 1 cut(s) 619
PctI GAATGC 4 cut(s) 126, 191, 259, 912
PdmI GAANNNNTTC 5 cut(s) 126, 146, 289, 555, 1574
PfeI GAWTC 7 cut(s) 277, 892, 934, 1397, 1687, 2093, 2164
PflMI CCANNNNNTGG 1 cut(s) 919
PkrI GCNGC 4 cut(s) 835, 1173, 1195, 1303
Ple19I CGATCG 1 cut(s) 1117
PleI GAGTC 1 cut(s) 1624
PpsI GAGTC 1 cut(s) 1624
PpuMI RGGWCCY 1 cut(s) 1105
PsiI TTATAA 1 cut(s) 1812
Psp1406I AACGTT 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 1105
Psp6I CCWGG 3 cut(s) 437, 853, 1510
PspFI CCCAGC 1 cut(s) 1353
PspGI CCWGG 3 cut(s) 437, 853, 1510
PspN4I GGNNCC 2 cut(s) 149, 1278
PspPI GGNCC 3 cut(s) 803, 1105, 1780
PspPPI RGGWCCY 1 cut(s) 1105
PspXI VCTCGAGB 1 cut(s) 1498
PstI CTGCAG 1 cut(s) 107
PsuI RGATCY 2 cut(s) 434, 1531
PvuI CGATCG 1 cut(s) 1117
PvuII CAGCTG 1 cut(s) 1673
RsaI GTAC 5 cut(s) 606, 715, 777, 1558, 2004
RsaNI GTAC 5 cut(s) 605, 714, 776, 1557, 2003
RseI CAYNNNNRTG 3 cut(s) 252, 1382, 1620
SaqAI TTAA 6 cut(s) 192, 650, 770, 1365, 1866, 2117
SatI GCNGC 4 cut(s) 834, 1172, 1194, 1302
Sau3AI GATC 8 cut(s) 400, 434, 780, 1114, 1386, 1531, 1675, 2011
Sau96I GGNCC 3 cut(s) 803, 1105, 1780
ScaI AGTACT 1 cut(s) 606
SchI GAGTC 1 cut(s) 1624
ScrFI CCNGG 3 cut(s) 439, 855, 1512
SfaNI GCATC 4 cut(s) 1003, 1381, 1434, 1789
SfcI CTRYAG 2 cut(s) 103, 1124
Sfr274I CTCGAG 1 cut(s) 1498
SfuI TTCGAA 1 cut(s) 1978
SinI GGWCC 2 cut(s) 1105, 1780
SlaI CTCGAG 1 cut(s) 1498
SmiMI CAYNNNNRTG 3 cut(s) 252, 1382, 1620
SmlI CTYRAG 2 cut(s) 1498, 1775
SmoI CTYRAG 2 cut(s) 1498, 1775
SphI GCATGC 1 cut(s) 1325
SsiI CCGC 4 cut(s) 733, 813, 1204, 1783
SspMI CTAG 8 cut(s) 23, 512, 584, 596, 747, 837, 1230, 1538
StyD4I CCNGG 3 cut(s) 437, 853, 1510
StyI CCWWGG 1 cut(s) 1402
TaaI ACNGT 7 cut(s) 90, 147, 335, 973, 1010, 1036, 1762
TaiI ACGT 4 cut(s) 616, 625, 770, 1575
TaqI TCGA 5 cut(s) 351, 1178, 1499, 1978, 2162
TatI WGTACW 2 cut(s) 604, 1556
TfiI GAWTC 7 cut(s) 277, 892, 934, 1397, 1687, 2093, 2164
Tru1I TTAA 6 cut(s) 192, 650, 770, 1365, 1866, 2117
Tru9I TTAA 6 cut(s) 192, 650, 770, 1365, 1866, 2117
TscAI CASTG 2 cut(s) 107, 1069
TseFI GTSAC 3 cut(s) 653, 1588, 2150
TseI GCWGC 4 cut(s) 833, 1171, 1193, 1301
Tsp45I GTSAC 3 cut(s) 653, 1588, 2150
TspGWI ACGGA 6 cut(s) 32, 444, 512, 968, 1861, 1902
TspRI CASTG 2 cut(s) 107, 1069
Van91I CCANNNNNTGG 1 cut(s) 919
VpaK11BI GGWCC 2 cut(s) 1105, 1780
XagI CCTNNNNNAGG 1 cut(s) 827
XapI RAATTY 3 cut(s) 223, 461, 1888
XbaI TCTAGA 1 cut(s) 595
XceI RCATGY 3 cut(s) 714, 1325, 1976
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XhoI CTCGAG 1 cut(s) 1498
XmiI GTMKAC 2 cut(s) 1443, 1603
XmnI GAANNNNTTC 5 cut(s) 126, 146, 289, 555, 1574
XspI CTAG 8 cut(s) 23, 512, 584, 596, 747, 837, 1230, 1538
ZraI GACGTC 1 cut(s) 623
ZrmI AGTACT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.