Prupe.1G158300_v2.0.a1

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000064
Physical Location & Seq
Forward (+)
12552549 .. 12554137
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G158300.1

Sequence Viewer

Length: 1257 bp
ATGTCTTGGACAGGTTGGTTTTGGGTTCAATATTCGTACGCTCTCATCACAAGACTAAGGAATAATAAGATTGACACTCGAGTGTCAGATCAATGGGCAAATCTTCCCGTTCTTTTTTTGATTCTGGATAACTTGCAGGAACCCACTGACCATATTCGGGTTGCTGCCGTTTGCAAGCAATGGCGTGTTGCTGCAAAAGAATACAATCGCATATCGCAGCGTTGGCAGCGTGAGCTTCTTCCTATGCTCCTGGTTCCAACCGCAACAGGATCCAAAGGAAAGCCAAAACTGTACAACTTTCCTGGAGGAGAAACCTCCGACATTGAGTTATCAGTGCCCTGTAATATGATTAGTATGTGCCATGGCCATGGTTGGTTAGCCACAATGGATGTATTGGAAAAACATCTGATAGTCGGTAAATCGAAATTTTTTTGGAACCAACATCTAGAACTGAGCAAACAAGAACTATGGACTATAACTCTGGTGAACCCTTTCAGAAAAGCAGCGGCGCCCATTCGTCTCCCTCGCTTGGGTTTCTGTTACCATCACTCGTTTGCGAGTCAAGGTTCGGATGAGCTCCGAAAGTGCGGGCTTGTACCGAAGCACATCCCGAAGGTTATCTTATCTGAGGATCCCACATTGAATCCAGACAGTTACATGGTTATAGCAGTTTGCAGACGTGTTTCTACGTTGGCTTTCACCAAAGGAGGAAGAAAATATTGGACTCAATTATTCACAGCGCGGGGTGTTATGGATGCTATATTTCATAAAAGTCTAGTCCTTGCAGTTTTAGATTTCGGAAACATTCTTTCCGTTGATATCAATAGCCGGTCAATGAAAACAAAGTATTTTAACCAGGAACGGTTCGAACGTAGGGCTGATGAAGCTTATCTCGTGGAATCAACCAAGGGAGACTTGTTGCACGTTCGAAGATTGCCGAAAGATGATCATGGTGGTAGTGGAAAGAGATTCATGGTTTACAAGTTGGTGTTCAATGAGAGGGGTGGATCCGTGCAGCATGTTGAAATGAAAAGTATCGGAGATGAGGTTTTGTTCCTGAGTGAAAATGGTAGTATCTCGGTTTTGGCTTCAAACTTTCCTCAGTGCCAACCCAATTCCATATATTACATGGATTTTCGTTTGCGGACTGAAATAAGCAATTTCAATTTAGAGGATGAAACCATTACACGATATCGTTACTCTTCGGACATCTCACAAGCGCTTTGGATTGTGGACATGTTTAATGGGCTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

419

Amino Acids

48.13

Weight (kDa)

9.12

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccII CGCG 1 cut(s) 742
AciI CCGC 5 cut(s) 261, 506, 588, 742, 1144
AclWI GGATC 6 cut(s) 264, 277, 626, 639, 1002, 1015
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 425
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 3 cut(s) 38, 293, 597
AfeI AGCGCT 1 cut(s) 1221
AfiI CCNNNNNNNGG 3 cut(s) 157, 529, 530
AflIII ACRYGT 2 cut(s) 679, 1236
AgsI TTSAA 6 cut(s) 29, 643, 994, 1025, 1092, 1165
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 3 cut(s) 249, 301, 855
AjuI GAANNNNNNNTTGG 2 cut(s) 703, 735
AleI CACNNNNGTG 1 cut(s) 80
AluBI AGCT 3 cut(s) 235, 577, 887
AluI AGCT 3 cut(s) 235, 577, 887
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 2 cut(s) 524, 906
AlwI GGATC 6 cut(s) 264, 277, 626, 639, 1002, 1015
Ama87I CYCGRG 1 cut(s) 78
Aor51HI AGCGCT 1 cut(s) 1221
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 6 cut(s) 164, 191, 217, 226, 503, 1015
ApoI RAATTY 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 491
AspLEI GCGC 3 cut(s) 511, 742, 1222
AsuHPI GGTGA 2 cut(s) 496, 691
AsuII TTCGAA 2 cut(s) 867, 928
AvaI CYCGRG 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 339
BalI TGGCCA 1 cut(s) 366
BamHI GGATCC 3 cut(s) 269, 631, 1007
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 2 cut(s) 579, 1251
BauI CACGAG 1 cut(s) 893
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 6 cut(s) 151, 178, 229, 238, 515, 1027
BccI CCATC 1 cut(s) 552
BceAI ACGGC 1 cut(s) 152
BciT130I CCWGG 3 cut(s) 251, 303, 857
BclI TGATCA 1 cut(s) 946
BcoDI GTCTC 2 cut(s) 524, 906
BfaI CTAG 3 cut(s) 446, 776, 1255
BfoI RGCGCY 2 cut(s) 512, 1223
BisI GCNGC 7 cut(s) 165, 192, 218, 227, 504, 507, 1016
BlsI GCNGC 7 cut(s) 166, 193, 219, 228, 505, 508, 1017
Bme1390I CCNGG 3 cut(s) 251, 303, 857
BmeT110I CYCGRG 1 cut(s) 78
BmgBI CACGTC 1 cut(s) 680
BmiI GGNNCC 7 cut(s) 141, 255, 271, 437, 510, 633, 1009
BmrFI CCNGG 3 cut(s) 251, 303, 857
BmsI GCATC 1 cut(s) 745
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 2 cut(s) 867, 928
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 3 cut(s) 361, 367, 906
BsaXI ACNNNNNCTCC 2 cut(s) 1032, 1062
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 529, 530
Bse118I RCCGGY 1 cut(s) 828
Bse3DI GCAATG 1 cut(s) 185
BseBI CCWGG 3 cut(s) 251, 303, 857
BseDI CCNNGG 3 cut(s) 361, 367, 906
BseGI GGATG 5 cut(s) 394, 577, 606, 760, 1180
BseLI CCNNNNNNNGG 3 cut(s) 157, 529, 530
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 4 cut(s) 443, 618, 1049, 1115
BseRI GAGGAG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 6 cut(s) 151, 178, 229, 238, 515, 1027
BsgI GTGCAG 1 cut(s) 1034
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 1 cut(s) 829
BsiWI CGTACG 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 157, 529, 530
BsmAI GTCTC 2 cut(s) 524, 906
BsmBI CGTCTC 1 cut(s) 524
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 2 cut(s) 867, 928
Bsp1286I GDGCHC 3 cut(s) 339, 579, 1251
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 5 cut(s) 88, 269, 631, 946, 1007
Bsp19I CCATGG 2 cut(s) 361, 367
BspACI CCGC 5 cut(s) 261, 506, 588, 742, 1144
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 4 cut(s) 444, 619, 1050, 1114
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 141, 255, 271, 437, 510, 633, 1009
BspPI GGATC 6 cut(s) 264, 277, 626, 639, 1002, 1015
BspT104I TTCGAA 2 cut(s) 867, 928
BspT107I GGYRCC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 3 cut(s) 361, 367, 906
BssMI GATC 5 cut(s) 88, 269, 631, 946, 1007
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 3 cut(s) 361, 367, 906
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 3 cut(s) 251, 303, 857
Bst4CI ACNGT 3 cut(s) 291, 653, 864
Bst6I CTCTTC 1 cut(s) 1207
BstACI GRCGYC 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 291
BstBI TTCGAA 2 cut(s) 867, 928
BstC8I GCNNGC 2 cut(s) 176, 590
BstDEI CTNAG 5 cut(s) 56, 452, 627, 1058, 1101
BstDSI CCRYGG 2 cut(s) 361, 367
BstF5I GGATG 5 cut(s) 394, 577, 606, 760, 1180
BstFNI CGCG 1 cut(s) 742
BstH2I RGCGCY 2 cut(s) 512, 1223
BstHHI GCGC 3 cut(s) 511, 742, 1222
BstKTI GATC 5 cut(s) 91, 272, 634, 949, 1010
BstMAI GTCTC 2 cut(s) 524, 906
BstMBI GATC 5 cut(s) 88, 269, 631, 946, 1007
BstMWI GCNNNNNNNGC 4 cut(s) 223, 226, 232, 884
BstNI CCWGG 3 cut(s) 251, 303, 857
BstNSI RCATGY 2 cut(s) 1022, 1240
BstSCI CCNGG 3 cut(s) 249, 301, 855
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 6 cut(s) 151, 178, 229, 238, 515, 1027
BstX2I RGATCY 3 cut(s) 269, 631, 1007
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 3 cut(s) 269, 631, 1007
BsuRI GGCC 1 cut(s) 366
BtgI CCRYGG 2 cut(s) 361, 367
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 5 cut(s) 394, 577, 606, 760, 1180
BtsIMutI CAGTG 3 cut(s) 144, 339, 1109
Cac8I GCNNGC 2 cut(s) 176, 590
CfoI GCGC 3 cut(s) 511, 742, 1222
Cfr10I RCCGGY 1 cut(s) 828
Csp6I GTAC 3 cut(s) 37, 292, 596
CviAII CATG 8 cut(s) 362, 368, 658, 950, 973, 1019, 1129, 1237
CviQI GTAC 3 cut(s) 37, 292, 596
DdeI CTNAG 5 cut(s) 56, 452, 627, 1058, 1101
DinI GGCGCC 1 cut(s) 510
DpnI GATC 5 cut(s) 90, 271, 633, 948, 1009
DpnII GATC 5 cut(s) 88, 269, 631, 946, 1007
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 1207
EarI CTCTTC 1 cut(s) 1207
Ecl136II GAGCTC 1 cut(s) 577
Eco130I CCWWGG 3 cut(s) 361, 367, 906
Eco24I GRGCYC 2 cut(s) 579, 1251
Eco32I GATATC 2 cut(s) 820, 1193
Eco47III AGCGCT 1 cut(s) 1221
Eco53kI GAGCTC 1 cut(s) 577
Eco88I CYCGRG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 577
EcoRII CCWGG 3 cut(s) 249, 301, 855
EcoRV GATATC 2 cut(s) 820, 1193
EcoT14I CCWWGG 3 cut(s) 361, 367, 906
EcoT38I GRGCYC 2 cut(s) 579, 1251
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
ErhI CCWWGG 3 cut(s) 361, 367, 906
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 8 cut(s) 365, 371, 661, 953, 976, 1022, 1132, 1240
FalI AAGNNNNNCTT 4 cut(s) 605, 637, 899, 931
FatI CATG 8 cut(s) 361, 367, 657, 949, 972, 1018, 1128, 1236
FauI CCCGC 2 cut(s) 581, 735
FbaI TGATCA 1 cut(s) 946
Fnu4HI GCNGC 7 cut(s) 165, 192, 218, 227, 504, 507, 1016
FokI GGATG 5 cut(s) 401, 584, 593, 767, 1187
FriOI GRGCYC 2 cut(s) 579, 1251
Fsp4HI GCNGC 7 cut(s) 165, 192, 218, 227, 504, 507, 1016
FspBI CTAG 3 cut(s) 446, 776, 1255
GlaI GCGC 3 cut(s) 510, 741, 1221
GluI GCNGC 7 cut(s) 165, 192, 218, 227, 504, 507, 1016
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 2 cut(s) 512, 1223
HaeIII GGCC 1 cut(s) 366
HapII CCGG 1 cut(s) 829
HhaI GCGC 3 cut(s) 511, 742, 1222
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 8 cut(s) 365, 371, 661, 953, 976, 1022, 1132, 1240
Hin6I GCGC 3 cut(s) 509, 740, 1220
HinP1I GCGC 3 cut(s) 509, 740, 1220
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 6 cut(s) 121, 559, 643, 724, 899, 969
HpaII CCGG 1 cut(s) 829
HphI GGTGA 2 cut(s) 496, 691
Hpy166II GTNNAC 3 cut(s) 487, 979, 1234
Hpy188III TCNNGA 5 cut(s) 125, 446, 610, 647, 1057
Hpy8I GTNNAC 3 cut(s) 487, 979, 1234
HpyAV CCTTC 1 cut(s) 607
HpyCH4III ACNGT 3 cut(s) 291, 653, 864
HpyCH4IV ACGT 4 cut(s) 679, 689, 871, 924
HpyCH4V TGCA 7 cut(s) 136, 174, 194, 675, 785, 922, 1015
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 226, 232, 884
HpyF3I CTNAG 5 cut(s) 56, 452, 627, 1058, 1101
HpySE526I ACGT 4 cut(s) 679, 689, 871, 924
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 8 cut(s) 365, 371, 661, 953, 976, 1022, 1132, 1240
HspAI GCGC 3 cut(s) 509, 740, 1220
KasI GGCGCC 1 cut(s) 508
Ksp22I TGATCA 1 cut(s) 946
Kzo9I GATC 5 cut(s) 88, 269, 631, 946, 1007
LmnI GCTCC 2 cut(s) 252, 582
Lsp1109I GCAGC 6 cut(s) 151, 178, 229, 238, 515, 1027
LweI GCATC 1 cut(s) 745
MaeI CTAG 3 cut(s) 446, 776, 1255
MaeII ACGT 4 cut(s) 679, 689, 871, 924
MaeIII GTNAC 3 cut(s) 539, 653, 1196
MalI GATC 5 cut(s) 90, 271, 633, 948, 1009
MboI GATC 5 cut(s) 88, 269, 631, 946, 1007
MboII GAAGA 5 cut(s) 95, 230, 723, 942, 1194
MflI RGATCY 3 cut(s) 269, 631, 1007
MhlI GDGCHC 3 cut(s) 339, 579, 1251
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 5 cut(s) 425, 728, 1114, 1159, 1165
MluNI TGGCCA 1 cut(s) 366
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 2 cut(s) 568, 718
MmeI TCCRAC 2 cut(s) 281, 342
MnlI CCTC 9 cut(s) 299, 325, 534, 622, 701, 993, 1039, 1110, 1165
Mox20I TGGCCA 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 491
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 852, 1242
MslI CAYNNNNRTG 2 cut(s) 80, 366
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 829
MspR9I CCNGG 3 cut(s) 251, 303, 857
MvaI CCWGG 3 cut(s) 251, 303, 857
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 4 cut(s) 223, 226, 232, 884
NarI GGCGCC 1 cut(s) 509
NcoI CCATGG 2 cut(s) 361, 367
NdeII GATC 5 cut(s) 88, 269, 631, 946, 1007
NlaIII CATG 8 cut(s) 365, 371, 661, 953, 976, 1022, 1132, 1240
NlaIV GGNNCC 7 cut(s) 141, 255, 271, 437, 510, 633, 1009
NspI RCATGY 2 cut(s) 1022, 1240
NspV TTCGAA 2 cut(s) 867, 928
OliI CACNNNNGTG 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 78
PciI ACATGT 1 cut(s) 1236
PcsI WCGNNNNNNNCGW 1 cut(s) 868
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 4 cut(s) 121, 643, 899, 969
Pfl23II CGTACG 1 cut(s) 36
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 7 cut(s) 166, 193, 219, 228, 505, 508, 1017
PleI GAGTC 2 cut(s) 567, 718
PluTI GGCGCC 1 cut(s) 512
PpsI GAGTC 2 cut(s) 567, 718
PscI ACATGT 1 cut(s) 1236
Psp124BI GAGCTC 1 cut(s) 579
Psp6I CCWGG 3 cut(s) 249, 301, 855
PspGI CCWGG 3 cut(s) 249, 301, 855
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 7 cut(s) 141, 255, 271, 437, 510, 633, 1009
PspXI VCTCGAGB 1 cut(s) 78
PsuI RGATCY 3 cut(s) 269, 631, 1007
RsaI GTAC 3 cut(s) 38, 293, 597
RsaNI GTAC 3 cut(s) 37, 292, 596
RseI CAYNNNNRTG 2 cut(s) 80, 366
SacI GAGCTC 1 cut(s) 579
SaqAI TTAA 2 cut(s) 852, 1242
SatI GCNGC 7 cut(s) 165, 192, 218, 227, 504, 507, 1016
Sau3AI GATC 5 cut(s) 88, 269, 631, 946, 1007
SchI GAGTC 2 cut(s) 568, 718
ScrFI CCNGG 3 cut(s) 251, 303, 857
SduI GDGCHC 3 cut(s) 339, 579, 1251
SfaNI GCATC 1 cut(s) 745
SfoI GGCGCC 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 78
SfuI TTCGAA 2 cut(s) 867, 928
SlaI CTCGAG 1 cut(s) 78
SmiMI CAYNNNNRTG 2 cut(s) 80, 366
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 5 cut(s) 425, 728, 1114, 1159, 1165
SsiI CCGC 5 cut(s) 261, 506, 588, 742, 1144
SspDI GGCGCC 1 cut(s) 508
SspI AATATT 2 cut(s) 32, 719
SspMI CTAG 3 cut(s) 446, 776, 1255
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 3 cut(s) 249, 301, 855
StyI CCWWGG 3 cut(s) 361, 367, 906
TaaI ACNGT 3 cut(s) 291, 653, 864
TaiI ACGT 4 cut(s) 682, 692, 874, 927
TaqI TCGA 4 cut(s) 79, 422, 867, 928
TasI AATT 5 cut(s) 425, 728, 1114, 1159, 1165
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 4 cut(s) 121, 643, 899, 969
Tru1I TTAA 2 cut(s) 852, 1242
Tru9I TTAA 2 cut(s) 852, 1242
TscAI CASTG 3 cut(s) 151, 339, 1109
TseI GCWGC 6 cut(s) 164, 191, 217, 226, 503, 1015
TspDTI ATGAA 6 cut(s) 755, 851, 897, 961, 1043, 1191
TspGWI ACGGA 2 cut(s) 802, 1000
TspRI CASTG 3 cut(s) 151, 339, 1109
XapI RAATTY 1 cut(s) 425
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 2 cut(s) 1022, 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 3 cut(s) 446, 776, 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.