Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1005 |
| AccB1I | GGYRCC | 1 cut(s) | 1179 |
| AccI | GTMKAC | 1 cut(s) | 597 |
| AccIII | TCCGGA | 1 cut(s) | 523 |
| AciI | CCGC | 3 cut(s) | 146, 185, 469 |
| AclWI | GGATC | 3 cut(s) | 205, 503, 661 |
| AcsI | RAATTY | 6 cut(s) | 564, 912, 998, 1034, 1170, 1189 |
| AfaI | GTAC | 1 cut(s) | 275 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 722, 932 |
| AflIII | ACRYGT | 1 cut(s) | 1305 |
| AgeI | ACCGGT | 2 cut(s) | 177, 1339 |
| AgsI | TTSAA | 3 cut(s) | 520, 865, 1039 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 195 |
| AjnI | CCWGG | 2 cut(s) | 292, 1029 |
| AluBI | AGCT | 6 cut(s) | 241, 434, 673, 691, 751, 1367 |
| AluI | AGCT | 6 cut(s) | 241, 434, 673, 691, 751, 1367 |
| Alw26I | GTCTC | 3 cut(s) | 154, 446, 1107 |
| AlwI | GGATC | 3 cut(s) | 205, 503, 661 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1458 |
| Aor13HI | TCCGGA | 1 cut(s) | 523 |
| AoxI | GGCC | 3 cut(s) | 296, 557, 625 |
| ApeKI | GCWGC | 4 cut(s) | 122, 392, 1087, 1367 |
| ApoI | RAATTY | 6 cut(s) | 564, 912, 998, 1034, 1170, 1189 |
| AseI | ATTAAT | 1 cut(s) | 1232 |
| AsiGI | ACCGGT | 2 cut(s) | 177, 1339 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 66, 419, 1307 |
| AspLEI | GCGC | 1 cut(s) | 312 |
| AspS9I | GGNCC | 3 cut(s) | 296, 378, 557 |
| AsuC2I | CCSGG | 1 cut(s) | 1223 |
| AsuHPI | GGTGA | 1 cut(s) | 1433 |
| AsuII | TTCGAA | 1 cut(s) | 994 |
| AvaII | GGWCC | 1 cut(s) | 378 |
| AxyI | CCTNAGG | 1 cut(s) | 1355 |
| BanI | GGYRCC | 1 cut(s) | 1179 |
| BbvI | GCAGC | 4 cut(s) | 109, 404, 1099, 1354 |
| BccI | CCATC | 8 cut(s) | 171, 462, 529, 584, 1007, 1064, 1149, 1327 |
| BceAI | ACGGC | 1 cut(s) | 110 |
| BciT130I | CCWGG | 2 cut(s) | 294, 1031 |
| BciVI | GTATCC | 3 cut(s) | 109, 352, 785 |
| BclI | TGATCA | 1 cut(s) | 679 |
| BcnI | CCSGG | 1 cut(s) | 1223 |
| BcoDI | GTCTC | 3 cut(s) | 154, 446, 1107 |
| BfaI | CTAG | 5 cut(s) | 300, 410, 825, 920, 1271 |
| BfmI | CTRYAG | 1 cut(s) | 1368 |
| BfoI | RGCGCY | 1 cut(s) | 313 |
| BfuI | GTATCC | 3 cut(s) | 109, 352, 785 |
| BisI | GCNGC | 5 cut(s) | 123, 393, 470, 1088, 1368 |
| BlsI | GCNGC | 5 cut(s) | 124, 394, 471, 1089, 1369 |
| Bme1390I | CCNGG | 3 cut(s) | 294, 1031, 1223 |
| Bme18I | GGWCC | 1 cut(s) | 378 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 195 |
| BmgT120I | GGNCC | 3 cut(s) | 296, 378, 557 |
| BmiI | GGNNCC | 3 cut(s) | 1078, 1181, 1361 |
| BmrFI | CCNGG | 3 cut(s) | 294, 1031, 1223 |
| BmrI | ACTGGG | 1 cut(s) | 879 |
| BmsI | GCATC | 5 cut(s) | 456, 598, 727, 1108, 1172 |
| BmuI | ACTGGG | 1 cut(s) | 879 |
| BpmI | CTGGAG | 4 cut(s) | 590, 604, 653, 779 |
| Bpu14I | TTCGAA | 1 cut(s) | 994 |
| BpuEI | CTTGAG | 1 cut(s) | 326 |
| BpuMI | CCSGG | 1 cut(s) | 1223 |
| BsaI | GGTCTC | 1 cut(s) | 154 |
| BsaJI | CCNNGG | 7 cut(s) | 15, 292, 293, 846, 879, 1080, 1175 |
| BsaWI | WCCGGW | 3 cut(s) | 177, 523, 1339 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 577, 607, 845, 875 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 722, 932 |
| Bse118I | RCCGGY | 2 cut(s) | 177, 1339 |
| Bse1I | ACTGG | 2 cut(s) | 587, 874 |
| Bse21I | CCTNAGG | 1 cut(s) | 1355 |
| Bse3DI | GCAATG | 2 cut(s) | 775, 889 |
| BseAI | TCCGGA | 1 cut(s) | 523 |
| BseBI | CCWGG | 2 cut(s) | 294, 1031 |
| BseDI | CCNNGG | 7 cut(s) | 15, 292, 293, 846, 879, 1080, 1175 |
| BseGI | GGATG | 1 cut(s) | 613 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 722, 932 |
| BseMI | GCAATG | 2 cut(s) | 775, 889 |
| BseMII | CTCAG | 1 cut(s) | 1369 |
| BseNI | ACTGG | 2 cut(s) | 587, 874 |
| BseRI | GAGGAG | 4 cut(s) | 863, 866, 869, 872 |
| BseXI | GCAGC | 4 cut(s) | 109, 404, 1099, 1354 |
| BsgI | GTGCAG | 1 cut(s) | 1106 |
| BshFI | GGCC | 3 cut(s) | 298, 559, 627 |
| BshNI | GGYRCC | 1 cut(s) | 1179 |
| BshTI | ACCGGT | 2 cut(s) | 177, 1339 |
| BsiSI | CCGG | 4 cut(s) | 178, 524, 1222, 1340 |
| BslFI | GGGAC | 2 cut(s) | 886, 1413 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 722, 932 |
| BsmAI | GTCTC | 3 cut(s) | 154, 446, 1107 |
| BsmBI | CGTCTC | 2 cut(s) | 446, 1107 |
| BsmFI | GGGAC | 2 cut(s) | 886, 1413 |
| BsmI | GAATGC | 1 cut(s) | 824 |
| BsnI | GGCC | 3 cut(s) | 298, 559, 627 |
| Bso31I | GGTCTC | 1 cut(s) | 154 |
| Bsp119I | TTCGAA | 1 cut(s) | 994 |
| Bsp13I | TCCGGA | 1 cut(s) | 523 |
| Bsp143I | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| Bsp19I | CCATGG | 2 cut(s) | 879, 1080 |
| BspACI | CCGC | 3 cut(s) | 146, 185, 469 |
| BspANI | GGCC | 3 cut(s) | 298, 559, 627 |
| BspCNI | CTCAG | 1 cut(s) | 1368 |
| BspEI | TCCGGA | 1 cut(s) | 523 |
| BspHI | TCATGA | 3 cut(s) | 207, 385, 423 |
| BspLI | GGNNCC | 3 cut(s) | 1078, 1181, 1361 |
| BspMAI | CTGCAG | 1 cut(s) | 1372 |
| BspPI | GGATC | 3 cut(s) | 205, 503, 661 |
| BspT104I | TTCGAA | 1 cut(s) | 994 |
| BspT107I | GGYRCC | 1 cut(s) | 1179 |
| BspTNI | GGTCTC | 1 cut(s) | 154 |
| BsrDI | GCAATG | 2 cut(s) | 775, 889 |
| BsrFI | RCCGGY | 2 cut(s) | 177, 1339 |
| BsrI | ACTGG | 2 cut(s) | 587, 874 |
| BssAI | RCCGGY | 2 cut(s) | 177, 1339 |
| BssECI | CCNNGG | 7 cut(s) | 15, 292, 293, 846, 879, 1080, 1175 |
| BssMI | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| BssT1I | CCWWGG | 3 cut(s) | 15, 879, 1080 |
| Bst2UI | CCWGG | 2 cut(s) | 294, 1031 |
| Bst4CI | ACNGT | 2 cut(s) | 1106, 1421 |
| Bst6I | CTCTTC | 1 cut(s) | 847 |
| BstBI | TTCGAA | 1 cut(s) | 994 |
| BstC8I | GCNNGC | 3 cut(s) | 144, 689, 1412 |
| BstDEI | CTNAG | 5 cut(s) | 727, 939, 972, 1249, 1355 |
| BstDSI | CCRYGG | 2 cut(s) | 879, 1080 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 930 |
| BstF5I | GGATG | 1 cut(s) | 613 |
| BstH2I | RGCGCY | 1 cut(s) | 313 |
| BstHHI | GCGC | 1 cut(s) | 312 |
| BstKTI | GATC | 7 cut(s) | 213, 337, 385, 511, 656, 682, 1276 |
| BstMAI | GTCTC | 3 cut(s) | 154, 446, 1107 |
| BstMBI | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| BstNI | CCWGG | 2 cut(s) | 294, 1031 |
| BstNSI | RCATGY | 4 cut(s) | 992, 1094, 1309, 1414 |
| BstSCI | CCNGG | 3 cut(s) | 292, 1029, 1221 |
| BstSFI | CTRYAG | 1 cut(s) | 1368 |
| BstV1I | GCAGC | 4 cut(s) | 109, 404, 1099, 1354 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1326 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1355 |
| BsuI | GTATCC | 3 cut(s) | 109, 352, 785 |
| BsuRI | GGCC | 3 cut(s) | 298, 559, 627 |
| BtgI | CCRYGG | 2 cut(s) | 879, 1080 |
| BtsCI | GGATG | 1 cut(s) | 613 |
| BtsIMutI | CAGTG | 1 cut(s) | 580 |
| Cac8I | GCNNGC | 3 cut(s) | 144, 689, 1412 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1458 |
| CciI | TCATGA | 3 cut(s) | 207, 385, 423 |
| CfoI | GCGC | 1 cut(s) | 312 |
| Cfr10I | RCCGGY | 2 cut(s) | 177, 1339 |
| Cfr13I | GGNCC | 3 cut(s) | 296, 378, 557 |
| Csp6I | GTAC | 1 cut(s) | 274 |
| CspAI | ACCGGT | 2 cut(s) | 177, 1339 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 627, 662 |
| CviQI | GTAC | 1 cut(s) | 274 |
| DdeI | CTNAG | 5 cut(s) | 727, 939, 972, 1249, 1355 |
| DpnI | GATC | 7 cut(s) | 212, 336, 384, 510, 655, 681, 1275 |
| DpnII | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| DraI | TTTAAA | 1 cut(s) | 549 |
| DriI | GACNNNNNGTC | 1 cut(s) | 195 |
| Eam1104I | CTCTTC | 1 cut(s) | 847 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 195 |
| EarI | CTCTTC | 1 cut(s) | 847 |
| Eco130I | CCWWGG | 3 cut(s) | 15, 879, 1080 |
| Eco31I | GGTCTC | 1 cut(s) | 154 |
| Eco32I | GATATC | 1 cut(s) | 899 |
| Eco47I | GGWCC | 1 cut(s) | 378 |
| Eco81I | CCTNAGG | 1 cut(s) | 1355 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 930 |
| EcoRI | GAATTC | 1 cut(s) | 1034 |
| EcoRII | CCWGG | 2 cut(s) | 292, 1029 |
| EcoRV | GATATC | 1 cut(s) | 899 |
| EcoT14I | CCWWGG | 3 cut(s) | 15, 879, 1080 |
| ErhI | CCWWGG | 3 cut(s) | 15, 879, 1080 |
| Esp3I | CGTCTC | 2 cut(s) | 446, 1107 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 942, 974 |
| FaqI | GGGAC | 2 cut(s) | 886, 1413 |
| FbaI | TGATCA | 1 cut(s) | 679 |
| FblI | GTMKAC | 1 cut(s) | 597 |
| Fnu4HI | GCNGC | 5 cut(s) | 123, 393, 470, 1088, 1368 |
| FokI | GGATG | 1 cut(s) | 620 |
| Fsp4HI | GCNGC | 5 cut(s) | 123, 393, 470, 1088, 1368 |
| FspBI | CTAG | 5 cut(s) | 300, 410, 825, 920, 1271 |
| GlaI | GCGC | 1 cut(s) | 311 |
| GluI | GCNGC | 5 cut(s) | 123, 393, 470, 1088, 1368 |
| GsuI | CTGGAG | 4 cut(s) | 590, 604, 653, 779 |
| HaeII | RGCGCY | 1 cut(s) | 313 |
| HaeIII | GGCC | 3 cut(s) | 298, 559, 627 |
| HapII | CCGG | 4 cut(s) | 178, 524, 1222, 1340 |
| HhaI | GCGC | 1 cut(s) | 312 |
| Hin6I | GCGC | 1 cut(s) | 310 |
| HinP1I | GCGC | 1 cut(s) | 310 |
| HindIII | AAGCTT | 1 cut(s) | 689 |
| HinfI | GANTC | 8 cut(s) | 32, 62, 79, 520, 761, 965, 1349, 1430 |
| HpaII | CCGG | 4 cut(s) | 178, 524, 1222, 1340 |
| HphI | GGTGA | 1 cut(s) | 1433 |
| Hpy166II | GTNNAC | 3 cut(s) | 598, 1045, 1102 |
| Hpy188I | TCNGA | 7 cut(s) | 43, 78, 220, 382, 817, 847, 1176 |
| Hpy8I | GTNNAC | 3 cut(s) | 598, 1045, 1102 |
| HpyAV | CCTTC | 3 cut(s) | 77, 225, 936 |
| HpyCH4III | ACNGT | 2 cut(s) | 1106, 1421 |
| HpyCH4IV | ACGT | 1 cut(s) | 786 |
| HpyF3I | CTNAG | 5 cut(s) | 727, 939, 972, 1249, 1355 |
| HpySE526I | ACGT | 1 cut(s) | 786 |
| HspAI | GCGC | 1 cut(s) | 310 |
| Kpn2I | TCCGGA | 1 cut(s) | 523 |
| Ksp22I | TGATCA | 1 cut(s) | 679 |
| Kzo9I | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| Lsp1109I | GCAGC | 4 cut(s) | 109, 404, 1099, 1354 |
| LweI | GCATC | 5 cut(s) | 456, 598, 727, 1108, 1172 |
| MaeI | CTAG | 5 cut(s) | 300, 410, 825, 920, 1271 |
| MaeII | ACGT | 1 cut(s) | 786 |
| MaeIII | GTNAC | 2 cut(s) | 576, 827 |
| MalI | GATC | 7 cut(s) | 212, 336, 384, 510, 655, 681, 1275 |
| MboI | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| MboII | GAAGA | 3 cut(s) | 47, 834, 1022 |
| MfeI | CAATTG | 1 cut(s) | 639 |
| MlyI | GAGTC | 1 cut(s) | 770 |
| MmeI | TCCRAC | 1 cut(s) | 1038 |
| MroI | TCCGGA | 1 cut(s) | 523 |
| MroXI | GAANNNNTTC | 3 cut(s) | 66, 419, 1307 |
| MseI | TTAA | 2 cut(s) | 548, 1232 |
| MspA1I | CMGCKG | 1 cut(s) | 673 |
| MspI | CCGG | 4 cut(s) | 178, 524, 1222, 1340 |
| MspR9I | CCNGG | 3 cut(s) | 294, 1031, 1223 |
| MunI | CAATTG | 1 cut(s) | 639 |
| Mva1269I | GAATGC | 1 cut(s) | 824 |
| MvaI | CCWGG | 2 cut(s) | 294, 1031 |
| NciI | CCSGG | 1 cut(s) | 1223 |
| NcoI | CCATGG | 2 cut(s) | 879, 1080 |
| NdeII | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| NlaIV | GGNNCC | 3 cut(s) | 1078, 1181, 1361 |
| NmuCI | GTSAC | 1 cut(s) | 827 |
| NspI | RCATGY | 4 cut(s) | 992, 1094, 1309, 1414 |
| NspV | TTCGAA | 1 cut(s) | 994 |
| PaeI | GCATGC | 1 cut(s) | 1414 |
| PagI | TCATGA | 3 cut(s) | 207, 385, 423 |
| PasI | CCCWGGG | 1 cut(s) | 293 |
| PciI | ACATGT | 1 cut(s) | 1305 |
| PctI | GAATGC | 1 cut(s) | 824 |
| PdmI | GAANNNNTTC | 3 cut(s) | 66, 419, 1307 |
| PfeI | GAWTC | 7 cut(s) | 32, 62, 79, 520, 965, 1349, 1430 |
| PinAI | ACCGGT | 2 cut(s) | 177, 1339 |
| PkrI | GCNGC | 5 cut(s) | 124, 394, 471, 1089, 1369 |
| PleI | GAGTC | 1 cut(s) | 769 |
| PpsI | GAGTC | 1 cut(s) | 769 |
| PscI | ACATGT | 1 cut(s) | 1305 |
| PshBI | ATTAAT | 1 cut(s) | 1232 |
| PsiI | TTATAA | 1 cut(s) | 1005 |
| Psp6I | CCWGG | 2 cut(s) | 292, 1029 |
| PspGI | CCWGG | 2 cut(s) | 292, 1029 |
| PspN4I | GGNNCC | 3 cut(s) | 1078, 1181, 1361 |
| PspPI | GGNCC | 3 cut(s) | 296, 378, 557 |
| PstI | CTGCAG | 1 cut(s) | 1372 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1458 |
| PvuII | CAGCTG | 1 cut(s) | 673 |
| RsaI | GTAC | 1 cut(s) | 275 |
| RsaNI | GTAC | 1 cut(s) | 274 |
| SaqAI | TTAA | 2 cut(s) | 548, 1232 |
| SatI | GCNGC | 5 cut(s) | 123, 393, 470, 1088, 1368 |
| Sau3AI | GATC | 7 cut(s) | 210, 334, 382, 508, 653, 679, 1273 |
| Sau96I | GGNCC | 3 cut(s) | 296, 378, 557 |
| SchI | GAGTC | 1 cut(s) | 770 |
| ScrFI | CCNGG | 3 cut(s) | 294, 1031, 1223 |
| SfaNI | GCATC | 5 cut(s) | 456, 598, 727, 1108, 1172 |
| SfcI | CTRYAG | 1 cut(s) | 1368 |
| SfuI | TTCGAA | 1 cut(s) | 994 |
| SinI | GGWCC | 1 cut(s) | 378 |
| SmlI | CTYRAG | 1 cut(s) | 305 |
| SmoI | CTYRAG | 1 cut(s) | 305 |
| SphI | GCATGC | 1 cut(s) | 1414 |
| SsiI | CCGC | 3 cut(s) | 146, 185, 469 |
| SspI | AATATT | 2 cut(s) | 349, 1295 |
| SspMI | CTAG | 5 cut(s) | 300, 410, 825, 920, 1271 |
| StyD4I | CCNGG | 3 cut(s) | 292, 1029, 1221 |
| StyI | CCWWGG | 3 cut(s) | 15, 879, 1080 |
| TaaI | ACNGT | 2 cut(s) | 1106, 1421 |
| TaiI | ACGT | 1 cut(s) | 789 |
| TaqI | TCGA | 3 cut(s) | 994, 1216, 1322 |
| TatI | WGTACW | 1 cut(s) | 273 |
| TauI | GCSGC | 1 cut(s) | 472 |
| TfiI | GAWTC | 7 cut(s) | 32, 62, 79, 520, 965, 1349, 1430 |
| Tru1I | TTAA | 2 cut(s) | 548, 1232 |
| Tru9I | TTAA | 2 cut(s) | 548, 1232 |
| TscAI | CASTG | 1 cut(s) | 587 |
| TseFI | GTSAC | 1 cut(s) | 827 |
| TseI | GCWGC | 4 cut(s) | 122, 392, 1087, 1367 |
| Tsp45I | GTSAC | 1 cut(s) | 827 |
| TspGWI | ACGGA | 1 cut(s) | 1077 |
| TspRI | CASTG | 1 cut(s) | 587 |
| VpaK11BI | GGWCC | 1 cut(s) | 378 |
| VspI | ATTAAT | 1 cut(s) | 1232 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 930 |
| XapI | RAATTY | 6 cut(s) | 564, 912, 998, 1034, 1170, 1189 |
| XceI | RCATGY | 4 cut(s) | 992, 1094, 1309, 1414 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1393 |
| XmiI | GTMKAC | 1 cut(s) | 597 |
| XmnI | GAANNNNTTC | 3 cut(s) | 66, 419, 1307 |
| XspI | CTAG | 5 cut(s) | 300, 410, 825, 920, 1271 |