Rw0G007050

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00264
OG0000064
Physical Location & Seq
Forward (+)
2540 .. 3409
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007050.1

Sequence Viewer

Length: 870 bp
ATGGATGAGAATTTCGTAGTAACGCTTATAAATCCATTATCCAAAGTTAAAGGGAGGAAAGCGAAAGAAAATTCAGTCATAGGCCTTCCTGCATTGAACCCTCCAAAGGATGATCGAGCCAGGGCTCAATGGTCTAGACAATGTGACCTTTATGTCTTCAAAGCTACAATGTCAGCGGACCCAATTTCAAATGTAAAGGATTGCATTGTTTTGGTCATTTACGAAGGATCGTGTCAAATGGCTTTTATTAGACTCGATAAAGACACAACATGGACTTATATTCATGCAGGGATTAGAAATGTTGAAGATGTTGTTTATTTTGAAGATAAATTCTACGCCGTCGATGATCTGAACCAGCTTTGGTATTTTTCCGTTACCACCACCCAATTCAAGTCGAGAGATGTAGATAGTGCAGTAGGAGCTGTTGTAGAAGATGACTTAATTAGGAGATATCTGGTAGAGTTAAATGATCACAAAGAACTAGTGATGGTTAAGAGGTATATGGATTTTGAACCTAATCGAGGTCATCTAATTGGCCGACGTAAGACAACAAAATTCCAAGTTTTCAAAATTAATGCTGATAAGCATGAGTGGACAGAGATAAAAACTATAGGTGATGTTGCTATTTTTGTGGGGGACTCTGTAGTATCGGTGTTGGCTTCAGATTTTCTTGGATGTCAAGCAGATTGCATATACTTCGTACATGATTGGGATCGTCAAAGACCTGGCTATGGAAGTCGTGGACCTCGTAATTTCGGCGTGTATAACCTCAAAACTCAACGCTTTTCACGGATTGGCACCGCAGCTGCAAACCTGATAAAGATGAGCAATCGAACACCAATTTGGGCTACGGCCAACATTGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

289

Amino Acids

33.13

Weight (kDa)

8.18

Isoelectric Point (pI)

23.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 7 - 256 2.4e-39 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04090 FvH4_1g04091 FvH4_1g04110 FvH4_1g04440 FvH4_1g05330 FvH4_1g05332 FvH4_1g05560 FvH4_1g05580 FvH4_1g05590 FvH4_1g05611 FvH4_1g05892 FvH4_1g05900 FvH4_1g06763 FvH4_1g17860 FvH4_1g17860 FvH4_1g17861 FvH4_1g17880 FvH4_1g17902 FvH4_2g08142 FvH4_2g25110 FvH4_2g34380 FvH4_3g19131 FvH4_3g20501 FvH4_4g02770 FvH4_4g02771 FvH4_4g17610 FvH4_4g26951 FvH4_4g28330 FvH4_4g29110 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29670 FvH4_4g29690 FvH4_4g29691 FvH4_4g30221 FvH4_4g30230 FvH4_4g30240 FvH4_4g30602 FvH4_4g30610 FvH4_6g01351 FvH4_6g01780 FvH4_6g01890 FvH4_6g47150 FvH4_6g52260 FvH4_6g52703 FvH4_7g23210 FvH4_7g23240 FvH4_7g23350 FvH4_7g28790 FvH4_7g28820 FvH4_7g28830 FvH4_7g28840 FvH4_7g28890 FvH4_7g29040 FvH4_7g29050 FvH4_7g29990 FvH4_7g33871 FvH4_c6g00090
malus_domestica MD01G1180800.v1.1 MD01G1199400.v1.1 MD02G1310000.v1.1 MD04G1097400.v1.1 MD07G1264800.v1.1 MD07G1264900.v1.1 MD07G1270200.v1.1 MD07G1270300.v1.1 MD07G1270500.v1.1 MD10G1043600.v1.1 MD13G1047500.v1.1 MD13G1075200.v1.1
prunus_persica Prupe.1G009400_v2.0.a1 Prupe.1G076100_v2.0.a1 Prupe.1G158300_v2.0.a1 Prupe.1G208900_v2.0.a1 Prupe.1G273900_v2.0.a1 Prupe.2G253900_v2.0.a1 Prupe.2G255700_v2.0.a1 Prupe.2G256000_v2.0.a1 Prupe.2G280300_v2.0.a1 Prupe.2G290700_v2.0.a1 Prupe.3G068500_v2.0.a1 Prupe.4G112800_v2.0.a1 Prupe.5G168600_v2.0.a1 Prupe.5G168800_v2.0.a1 Prupe.5G169100_v2.0.a1 Prupe.8G049800_v2.0.a1
pyrus_communis pycom01g19330 pycom01g20930 pycom01g22010 pycom01g22020 pycom02g26090 pycom07g24330 pycom07g24720 pycom13g04150 pycom420g01320
rosa_chinensis RchiOBHm_Chr1g0324121 RchiOBHm_Chr1g0324251 RchiOBHm_Chr1g0343741 RchiOBHm_Chr1g0355031 RchiOBHm_Chr1g0359461 RchiOBHm_Chr1g0376351 RchiOBHm_Chr1g0376381 RchiOBHm_Chr2g0092531 RchiOBHm_Chr2g0108311 RchiOBHm_Chr2g0108321 RchiOBHm_Chr2g0108331 RchiOBHm_Chr2g0133991 RchiOBHm_Chr2g0156691 RchiOBHm_Chr2g0173801 RchiOBHm_Chr3g0449701 RchiOBHm_Chr3g0449861 RchiOBHm_Chr4g0417201 RchiOBHm_Chr4g0417931 RchiOBHm_Chr4g0424551 RchiOBHm_Chr4g0442921 RchiOBHm_Chr4g0442931 RchiOBHm_Chr5g0032171 RchiOBHm_Chr5g0032191 RchiOBHm_Chr5g0032221 RchiOBHm_Chr6g0258051 RchiOBHm_Chr6g0277851 RchiOBHm_Chr6g0291671 RchiOBHm_Chr6g0291691 RchiOBHm_Chr6g0312761 RchiOBHm_Chr7g0223801 RchiOBHm_Chr7g0224141
rosa_laevigata RLG00000001917 RLG00000001951 RLG00000005804 RLG00000006309 RLG00000006344 RLG00000006458 RLG00000016168 RLG00000016259 RLG00000016305 RLG00000016306 RLG00000019810 RLG00000022223 RLG00000026077 RLG00000026607 RLG00000029703 RLG00000033364 RLG00000034633 RLG00000034637
rosa_multiflora Rmu_co8010764.1_g000001 Rmu_co8262143.1_g000001 Rmu_co8346175.1_g000001 Rmu_sc0000096.1_g000016 Rmu_sc0000544.1_g000003 Rmu_sc0000591.1_g000007 Rmu_sc0000756.1_g000029 Rmu_sc0000841.1_g000017 Rmu_sc0000907.1_g000020 Rmu_sc0000954.1_g000004 Rmu_sc0001195.1_g000007 Rmu_sc0001198.1_g000008 Rmu_sc0001201.1_g000011 Rmu_sc0001201.1_g000013 Rmu_sc0001782.1_g000035 Rmu_sc0001904.1_g000004 Rmu_sc0001962.1_g000039 Rmu_sc0002113.1_g000021 Rmu_sc0002226.1_g000017 Rmu_sc0002226.1_g000020 Rmu_sc0002327.1_g000005 Rmu_sc0002372.1_g000010 Rmu_sc0002372.1_g000014 Rmu_sc0002687.1_g000005 Rmu_sc0003031.1_g000019 Rmu_sc0003076.1_g000014 Rmu_sc0004296.1_g000009 Rmu_sc0004540.1_g000009 Rmu_sc0005369.1_g000002 Rmu_sc0005375.1_g000003 Rmu_sc0005375.1_g000013 Rmu_sc0005742.1_g000008 Rmu_sc0005742.1_g000015 Rmu_sc0005742.1_g000021 Rmu_sc0007419.1_g000011 Rmu_sc0008909.1_g000008 Rmu_sc0010556.1_g000018 Rmu_sc0010999.1_g000008 Rmu_sc0017510.1_g000003 Rmu_sc0017556.1_g000001 Rmu_sc0017900.1_g000007 Rmu_sc0024249.1_g000001 Rmu_sc0032377.1_g000001 Rmu_sc0033569.1_g000001 Rmu_sc0036457.1_g000001 Rmu_sc0041304.1_g000001 Rmu_ssc0000204.1_g000011 Rmu_ssc0000438.1_g000056
rosa_roxburghii Rroxscaffold_1G00048250 Rroxscaffold_1G00048290 Rroxscaffold_2G00092800 Rroxscaffold_2G00110580 Rroxscaffold_2G00135240 Rroxscaffold_2G00135260 Rroxscaffold_2G00135300 Rroxscaffold_2G00149010 Rroxscaffold_2G00149160 Rroxscaffold_2G00149170 Rroxscaffold_2G00149630 Rroxscaffold_2G00151760 Rroxscaffold_2G00151790 Rroxscaffold_2G00152170 Rroxscaffold_3G00225510 Rroxscaffold_3G00249210 Rroxscaffold_4G00277020 Rroxscaffold_4G00281760 Rroxscaffold_4G00281770 Rroxscaffold_4G00281810 Rroxscaffold_4G00281820 Rroxscaffold_4G00282160 Rroxscaffold_4G00310340 Rroxscaffold_5G00361040 Rroxscaffold_5G00363770 Rroxscaffold_5G00366460 Rroxscaffold_5G00378270 Rroxscaffold_5G00378310 Rroxscaffold_5G00379550 Rroxscaffold_5G00379650 Rroxscaffold_6G00422110 Rroxscaffold_6G00427680 Rroxscaffold_7G00175850 Rroxscaffold_7G00175860 Rroxscaffold_7G00190720 Rroxscaffold_7G00190750 Rroxscaffold_7G00190830 Rroxscaffold_7G00196820
rosa_rugosa Rorug01G0395900 Rorug01G0444400 Rorug01G0488100 Rorug02G0001700 Rorug02G0021600 Rorug02G0025700 Rorug02G0152200 Rorug02G0271300 Rorug02G0455800 Rorug02G0572800 Rorug02G0576000 Rorug04G0146300 Rorug04G0172200 Rorug04G0172400 Rorug04G0311300 Rorug04G0312200 Rorug04G0312300 Rorug05G0129500 Rorug05G0129700 Rorug05G0250600 Rorug06G0112600 Rorug06G0220700 Rorug06G0220800 Rorug07G0114600
rosa_samantha Rh1DG066700 Rh1DG081800 Rh1DG184300 Rh1DG401900 Rh1DG402700 Rh1DG407000 Rh1DG407600 Rh2AG200800 Rh2AG201000 Rh2AG521000 Rh2BG066200 Rh2BG066300 Rh2BG073900 Rh2BG373500 Rh2BG417600 Rh2BG662600 Rh2DG066200 Rh2DG072000 Rh2DG390000 Rh2DG677700 Rh3CG020200 Rh3CG021500 Rh3CG081200 Rh4AG209600 Rh4AG362600 Rh4AG362700 Rh4AG397200 Rh4BG209800 Rh4BG209900 Rh4BG210000 Rh4CG220800 Rh4CG271300 Rh4CG388000 Rh4DG029800 Rh4DG368700 Rh4DG403900 Rh5AG035900 Rh5BG034600 Rh5BG034900 Rh5DG228700 Rh5DG228800 Rh6AG355900 Rh6CG083600 Rh6CG229000 Rh6CG229100 Rh6CG229700 Rh6CG346500 Rh6CG346600 Rh7AG362300 Rh7AG362400 Rh7AG363200 Rh7AG365100 Rh7CG380900 Rh7CG381000 Rh7CG381600 Rh7DG437600
rosa_wichuraiana Rw0G004580 Rw0G004590 Rw0G005750 Rw0G006640 Rw0G007050 Rw0G008040 Rw0G012200 Rw0G013750 Rw0G016590 Rw1G006090 Rw1G012040 Rw1G036130 Rw1G036270 Rw1G036290 Rw1G036420 Rw1G036430 Rw1G036590 Rw2G003560 Rw2G005130 Rw2G005980 Rw2G006090 Rw2G015980 Rw2G016000 Rw2G043010 Rw2G053520 Rw4G018760 Rw4G022000 Rw4G034250 Rw5G003280 Rw5G020380 Rw5G020390 Rw6G008210 Rw6G031000 Rw7G030830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 29, 868
AasI GACNNNNNNGTC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 797
AciI CCGC 2 cut(s) 176, 801
AclWI GGATC 2 cut(s) 235, 720
AcoI YGGCCR 2 cut(s) 535, 852
AcsI RAATTY 4 cut(s) 10, 70, 329, 554
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 3 cut(s) 106, 521, 731
AgsI TTSAA 9 cut(s) 97, 160, 189, 305, 323, 391, 512, 568, 863
AhlI ACTAGT 1 cut(s) 481
AjnI CCWGG 2 cut(s) 119, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AluBI AGCT 4 cut(s) 164, 358, 422, 806
AluI AGCT 4 cut(s) 164, 358, 422, 806
AlwI GGATC 2 cut(s) 235, 720
AoxI GGCC 3 cut(s) 82, 535, 852
ApeKI GCWGC 2 cut(s) 803, 806
ApoI RAATTY 4 cut(s) 10, 70, 329, 554
AseI ATTAAT 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 178, 743
AsuHPI GGTGA 1 cut(s) 626
AvaII GGWCC 2 cut(s) 178, 743
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 793, 815
BccI CCATC 1 cut(s) 481
BceAI ACGGC 2 cut(s) 323, 867
BcgI CGANNNNNNTGC 2 cut(s) 679, 713
BciT130I CCWGG 2 cut(s) 121, 726
BclI TGATCA 1 cut(s) 469
BcuI ACTAGT 1 cut(s) 481
BfaI CTAG 2 cut(s) 135, 482
BfmI CTRYAG 2 cut(s) 609, 642
BisI GCNGC 2 cut(s) 804, 807
BlsI GCNGC 2 cut(s) 805, 808
Bme1390I CCNGG 2 cut(s) 121, 726
Bme18I GGWCC 2 cut(s) 178, 743
BmgT120I GGNCC 2 cut(s) 178, 743
BmiI GGNNCC 2 cut(s) 180, 799
BmrFI CCNGG 2 cut(s) 121, 726
BpiI GAAGAC 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 521, 731
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 2 cut(s) 121, 726
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 3 cut(s) 10, 115, 680
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 3 cut(s) 106, 521, 731
BseXI GCAGC 2 cut(s) 793, 815
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 3 cut(s) 84, 537, 854
BshNI GGYRCC 1 cut(s) 797
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 3 cut(s) 106, 521, 731
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 3 cut(s) 84, 537, 854
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 5 cut(s) 112, 227, 346, 469, 712
BspACI CCGC 2 cut(s) 176, 801
BspANI GGCC 3 cut(s) 84, 537, 854
BspLI GGNNCC 2 cut(s) 180, 799
BspPI GGATC 2 cut(s) 235, 720
BspT107I GGYRCC 1 cut(s) 797
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 5 cut(s) 112, 227, 346, 469, 712
Bst2UI CCWGG 2 cut(s) 121, 726
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 10, 115, 680
BstKTI GATC 5 cut(s) 115, 230, 349, 472, 715
BstMBI GATC 5 cut(s) 112, 227, 346, 469, 712
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 2 cut(s) 121, 726
BstSCI CCNGG 2 cut(s) 119, 724
BstSFI CTRYAG 2 cut(s) 609, 642
BstV1I GCAGC 2 cut(s) 793, 815
BstV2I GAAGAC 1 cut(s) 148
BsuRI GGCC 3 cut(s) 84, 537, 854
BtsCI GGATG 3 cut(s) 10, 115, 680
Cfr13I GGNCC 2 cut(s) 178, 743
Csp6I GTAC 1 cut(s) 701
CviAII CATG 4 cut(s) 270, 284, 587, 704
CviQI GTAC 1 cut(s) 701
DpnI GATC 5 cut(s) 114, 229, 348, 471, 714
DpnII GATC 5 cut(s) 112, 227, 346, 469, 712
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 2 cut(s) 535, 852
Eco147I AGGCCT 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 127
Eco32I GATATC 1 cut(s) 452
Eco47I GGWCC 2 cut(s) 178, 743
Eco57I CTGAAG 1 cut(s) 645
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 119, 724
EcoRV GATATC 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 127
FaeI CATG 4 cut(s) 273, 287, 590, 707
FaqI GGGAC 1 cut(s) 650
FatI CATG 4 cut(s) 269, 283, 586, 703
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 804, 807
FokI GGATG 3 cut(s) 17, 122, 687
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 2 cut(s) 804, 807
FspBI CTAG 2 cut(s) 135, 482
GluI GCNGC 2 cut(s) 804, 807
HaeIII GGCC 3 cut(s) 84, 537, 854
Hin1II CATG 4 cut(s) 273, 287, 590, 707
HinfI GANTC 2 cut(s) 252, 638
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 2 cut(s) 594, 743
Hpy188I TCNGA 2 cut(s) 351, 664
Hpy188III TCNNGA 2 cut(s) 135, 396
Hpy8I GTNNAC 2 cut(s) 594, 743
Hpy99I CGWCG 2 cut(s) 344, 543
HpyAV CCTTC 2 cut(s) 95, 218
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 6 cut(s) 92, 204, 287, 413, 690, 809
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 4 cut(s) 273, 287, 590, 707
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 5 cut(s) 112, 227, 346, 469, 712
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 9 cut(s) 102, 106, 133, 273, 368, 440, 711, 738, 827
Lsp1109I GCAGC 2 cut(s) 793, 815
MaeI CTAG 2 cut(s) 135, 482
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 3 cut(s) 19, 143, 373
MalI GATC 5 cut(s) 114, 229, 348, 471, 714
MboI GATC 5 cut(s) 112, 227, 346, 469, 712
MboII GAAGA 4 cut(s) 148, 317, 335, 443
MhlI GDGCHC 1 cut(s) 127
MlyI GAGTC 2 cut(s) 246, 632
MnlI CCTC 6 cut(s) 48, 111, 489, 515, 756, 779
MseI TTAA 5 cut(s) 48, 440, 464, 492, 573
MspA1I CMGCKG 2 cut(s) 176, 806
MspR9I CCNGG 2 cut(s) 121, 726
MvaI CCWGG 2 cut(s) 121, 726
MwoI GCNNNNNNNGC 1 cut(s) 419
NdeII GATC 5 cut(s) 112, 227, 346, 469, 712
NlaIII CATG 4 cut(s) 273, 287, 590, 707
NlaIV GGNNCC 2 cut(s) 180, 799
NmuCI GTSAC 1 cut(s) 143
PceI AGGCCT 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 745
PkrI GCNGC 2 cut(s) 805, 808
PleI GAGTC 2 cut(s) 246, 632
PpsI GAGTC 2 cut(s) 246, 632
PshBI ATTAAT 1 cut(s) 573
PsiI TTATAA 2 cut(s) 29, 868
Psp6I CCWGG 2 cut(s) 119, 724
PspGI CCWGG 2 cut(s) 119, 724
PspN4I GGNNCC 2 cut(s) 180, 799
PspPI GGNCC 2 cut(s) 178, 743
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SaqAI TTAA 5 cut(s) 48, 440, 464, 492, 573
SatI GCNGC 2 cut(s) 804, 807
Sau3AI GATC 5 cut(s) 112, 227, 346, 469, 712
Sau96I GGNCC 2 cut(s) 178, 743
SchI GAGTC 2 cut(s) 246, 632
ScrFI CCNGG 2 cut(s) 121, 726
SduI GDGCHC 1 cut(s) 127
SfcI CTRYAG 2 cut(s) 609, 642
SinI GGWCC 2 cut(s) 178, 743
SpeI ACTAGT 1 cut(s) 481
SseBI AGGCCT 1 cut(s) 84
SsiI CCGC 2 cut(s) 176, 801
SspMI CTAG 2 cut(s) 135, 482
StuI AGGCCT 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 119, 724
TaiI ACGT 1 cut(s) 544
TaqI TCGA 6 cut(s) 115, 255, 342, 395, 520, 832
Tru1I TTAA 5 cut(s) 48, 440, 464, 492, 573
Tru9I TTAA 5 cut(s) 48, 440, 464, 492, 573
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 2 cut(s) 803, 806
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 2 cut(s) 361, 805
VpaK11BI GGWCC 2 cut(s) 178, 743
VspI ATTAAT 1 cut(s) 573
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 4 cut(s) 10, 70, 329, 554
XbaI TCTAGA 1 cut(s) 134
XspI CTAG 2 cut(s) 135, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.