Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 195 |
| AccB1I | GGYRCC | 2 cut(s) | 466, 1222 |
| AccI | GTMKAC | 2 cut(s) | 492, 954 |
| AccIII | TCCGGA | 1 cut(s) | 565 |
| AciI | CCGC | 2 cut(s) | 546, 1254 |
| AclI | AACGTT | 1 cut(s) | 1207 |
| AclWI | GGATC | 5 cut(s) | 125, 138, 545, 733, 911 |
| AcsI | RAATTY | 1 cut(s) | 1093 |
| AcuI | CTGAAG | 2 cut(s) | 43, 436 |
| AdeI | CACNNNGTG | 1 cut(s) | 262 |
| AfaI | GTAC | 5 cut(s) | 266, 296, 654, 932, 1192 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| AflIII | ACRYGT | 1 cut(s) | 1164 |
| AgsI | TTSAA | 8 cut(s) | 308, 422, 562, 595, 671, 1071, 1153, 1177 |
| AjiI | CACGTC | 1 cut(s) | 1165 |
| AjnI | CCWGG | 1 cut(s) | 919 |
| AluBI | AGCT | 4 cut(s) | 14, 439, 710, 945 |
| AluI | AGCT | 4 cut(s) | 14, 439, 710, 945 |
| Alw21I | GWGCWC | 1 cut(s) | 911 |
| Alw26I | GTCTC | 3 cut(s) | 36, 741, 825 |
| Alw44I | GTGCAC | 1 cut(s) | 907 |
| AlwI | GGATC | 5 cut(s) | 125, 138, 545, 733, 911 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 78 |
| Aor13HI | TCCGGA | 1 cut(s) | 565 |
| AoxI | GGCC | 1 cut(s) | 300 |
| ApaLI | GTGCAC | 1 cut(s) | 907 |
| ApeKI | GCWGC | 4 cut(s) | 63, 66, 179, 182 |
| ApoI | RAATTY | 1 cut(s) | 1093 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 621, 653, 846, 878 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 449 |
| AsuHPI | GGTGA | 5 cut(s) | 686, 973, 1052, 1079, 1189 |
| AsuII | TTCGAA | 1 cut(s) | 850 |
| BaeGI | GKGCMC | 1 cut(s) | 911 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1153, 1186 |
| BamHI | GGATCC | 1 cut(s) | 130 |
| BanI | GGYRCC | 2 cut(s) | 466, 1222 |
| BauI | CACGAG | 1 cut(s) | 440 |
| BbsI | GAAGAC | 2 cut(s) | 80, 859 |
| Bbv12I | GWGCWC | 1 cut(s) | 911 |
| BbvI | GCAGC | 4 cut(s) | 75, 78, 166, 169 |
| BccI | CCATC | 3 cut(s) | 150, 346, 1202 |
| BceAI | ACGGC | 1 cut(s) | 16 |
| BciT130I | CCWGG | 1 cut(s) | 921 |
| BcoDI | GTCTC | 3 cut(s) | 36, 741, 825 |
| BfaI | CTAG | 3 cut(s) | 252, 711, 1264 |
| BfmI | CTRYAG | 1 cut(s) | 767 |
| BisI | GCNGC | 4 cut(s) | 64, 67, 180, 183 |
| BlsI | GCNGC | 4 cut(s) | 65, 68, 181, 184 |
| BmcAI | AGTACT | 1 cut(s) | 932 |
| Bme1390I | CCNGG | 1 cut(s) | 921 |
| BmgBI | CACGTC | 1 cut(s) | 1165 |
| BmiI | GGNNCC | 3 cut(s) | 132, 468, 1224 |
| BmrFI | CCNGG | 1 cut(s) | 921 |
| BmrI | ACTGGG | 1 cut(s) | 88 |
| BmsI | GCATC | 3 cut(s) | 78, 217, 534 |
| BmuI | ACTGGG | 1 cut(s) | 88 |
| BpiI | GAAGAC | 2 cut(s) | 80, 859 |
| Bpu14I | TTCGAA | 1 cut(s) | 850 |
| Bsa29I | ATCGAT | 1 cut(s) | 728 |
| BsaJI | CCNNGG | 2 cut(s) | 825, 920 |
| BsaWI | WCCGGW | 1 cut(s) | 565 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1253 |
| Bse1I | ACTGG | 1 cut(s) | 83 |
| Bse3DI | GCAATG | 2 cut(s) | 468, 502 |
| BseAI | TCCGGA | 1 cut(s) | 565 |
| BseBI | CCWGG | 1 cut(s) | 921 |
| BseCI | ATCGAT | 1 cut(s) | 728 |
| BseDI | CCNNGG | 2 cut(s) | 825, 920 |
| BseGI | GGATG | 2 cut(s) | 369, 724 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| BseMI | GCAATG | 2 cut(s) | 468, 502 |
| BseNI | ACTGG | 1 cut(s) | 83 |
| BseSI | GKGCMC | 1 cut(s) | 911 |
| BseXI | GCAGC | 4 cut(s) | 75, 78, 166, 169 |
| BseYI | CCCAGC | 1 cut(s) | 14 |
| BshFI | GGCC | 1 cut(s) | 302 |
| BshNI | GGYRCC | 2 cut(s) | 466, 1222 |
| BshVI | ATCGAT | 1 cut(s) | 728 |
| BsiHKAI | GWGCWC | 1 cut(s) | 911 |
| BsiSI | CCGG | 1 cut(s) | 566 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| BsmAI | GTCTC | 3 cut(s) | 36, 741, 825 |
| BsmI | GAATGC | 1 cut(s) | 612 |
| BsnI | GGCC | 1 cut(s) | 302 |
| Bsp119I | TTCGAA | 1 cut(s) | 850 |
| Bsp1286I | GDGCHC | 1 cut(s) | 911 |
| Bsp13I | TCCGGA | 1 cut(s) | 565 |
| Bsp143I | GATC | 4 cut(s) | 130, 550, 725, 916 |
| BspACI | CCGC | 2 cut(s) | 546, 1254 |
| BspANI | GGCC | 1 cut(s) | 302 |
| BspDI | ATCGAT | 1 cut(s) | 728 |
| BspEI | TCCGGA | 1 cut(s) | 565 |
| BspLI | GGNNCC | 3 cut(s) | 132, 468, 1224 |
| BspPI | GGATC | 5 cut(s) | 125, 138, 545, 733, 911 |
| BspT104I | TTCGAA | 1 cut(s) | 850 |
| BspT107I | GGYRCC | 2 cut(s) | 466, 1222 |
| BsrDI | GCAATG | 2 cut(s) | 468, 502 |
| BsrI | ACTGG | 1 cut(s) | 83 |
| BssECI | CCNNGG | 2 cut(s) | 825, 920 |
| BssMI | GATC | 4 cut(s) | 130, 550, 725, 916 |
| BssNAI | GTATAC | 1 cut(s) | 955 |
| BssSI | CACGAG | 1 cut(s) | 440 |
| BssT1I | CCWWGG | 1 cut(s) | 825 |
| Bst1107I | GTATAC | 1 cut(s) | 955 |
| Bst2BI | CACGAG | 1 cut(s) | 440 |
| Bst2UI | CCWGG | 1 cut(s) | 921 |
| Bst4CI | ACNGT | 3 cut(s) | 294, 572, 1248 |
| Bst6I | CTCTTC | 2 cut(s) | 34, 853 |
| BstBI | TTCGAA | 1 cut(s) | 850 |
| BstC8I | GCNNGC | 2 cut(s) | 215, 612 |
| BstDEI | CTNAG | 1 cut(s) | 760 |
| BstF5I | GGATG | 2 cut(s) | 369, 724 |
| BstKTI | GATC | 4 cut(s) | 133, 553, 728, 919 |
| BstMAI | GTCTC | 3 cut(s) | 36, 741, 825 |
| BstMBI | GATC | 4 cut(s) | 130, 550, 725, 916 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 214 |
| BstNI | CCWGG | 1 cut(s) | 921 |
| BstNSI | RCATGY | 1 cut(s) | 848 |
| BstSCI | CCNGG | 1 cut(s) | 919 |
| BstSFI | CTRYAG | 1 cut(s) | 767 |
| BstSLI | GKGCMC | 1 cut(s) | 911 |
| BstV1I | GCAGC | 4 cut(s) | 75, 78, 166, 169 |
| BstV2I | GAAGAC | 2 cut(s) | 80, 859 |
| BstX2I | RGATCY | 1 cut(s) | 130 |
| BstYI | RGATCY | 1 cut(s) | 130 |
| BstZ17I | GTATAC | 1 cut(s) | 955 |
| Bsu15I | ATCGAT | 1 cut(s) | 728 |
| BsuRI | GGCC | 1 cut(s) | 302 |
| BsuTUI | ATCGAT | 1 cut(s) | 728 |
| BtgZI | GCGATG | 2 cut(s) | 194, 1158 |
| BtrI | CACGTC | 1 cut(s) | 1165 |
| BtsCI | GGATG | 2 cut(s) | 369, 724 |
| Cac8I | GCNNGC | 2 cut(s) | 215, 612 |
| CaiI | CAGNNNCTG | 1 cut(s) | 78 |
| ClaI | ATCGAT | 1 cut(s) | 728 |
| Csp6I | GTAC | 5 cut(s) | 265, 295, 653, 931, 1191 |
| CviAII | CATG | 4 cut(s) | 46, 235, 521, 845 |
| CviQI | GTAC | 5 cut(s) | 265, 295, 653, 931, 1191 |
| DdeI | CTNAG | 1 cut(s) | 760 |
| DpnI | GATC | 4 cut(s) | 132, 552, 727, 918 |
| DpnII | GATC | 4 cut(s) | 130, 550, 725, 916 |
| DraIII | CACNNNGTG | 1 cut(s) | 262 |
| Eam1104I | CTCTTC | 2 cut(s) | 34, 853 |
| EarI | CTCTTC | 2 cut(s) | 34, 853 |
| EciI | GGCGGA | 1 cut(s) | 535 |
| Eco130I | CCWWGG | 1 cut(s) | 825 |
| Eco57I | CTGAAG | 2 cut(s) | 43, 436 |
| EcoRII | CCWGG | 1 cut(s) | 919 |
| EcoT14I | CCWWGG | 1 cut(s) | 825 |
| ErhI | CCWWGG | 1 cut(s) | 825 |
| FaeI | CATG | 4 cut(s) | 49, 238, 524, 848 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 818, 850, 1063, 1095 |
| FatI | CATG | 4 cut(s) | 45, 234, 520, 844 |
| FblI | GTMKAC | 2 cut(s) | 492, 954 |
| Fnu4HI | GCNGC | 4 cut(s) | 64, 67, 180, 183 |
| FokI | GGATG | 2 cut(s) | 376, 731 |
| Fsp4HI | GCNGC | 4 cut(s) | 64, 67, 180, 183 |
| FspBI | CTAG | 3 cut(s) | 252, 711, 1264 |
| GluI | GCNGC | 4 cut(s) | 64, 67, 180, 183 |
| GsaI | CCCAGC | 1 cut(s) | 18 |
| HaeIII | GGCC | 1 cut(s) | 302 |
| HapII | CCGG | 1 cut(s) | 566 |
| Hin1II | CATG | 4 cut(s) | 49, 238, 524, 848 |
| HinfI | GANTC | 6 cut(s) | 112, 562, 643, 667, 699, 1115 |
| HpaII | CCGG | 1 cut(s) | 566 |
| HphI | GGTGA | 5 cut(s) | 686, 973, 1052, 1079, 1189 |
| Hpy166II | GTNNAC | 5 cut(s) | 265, 445, 493, 909, 955 |
| Hpy188I | TCNGA | 5 cut(s) | 93, 277, 455, 515, 1120 |
| Hpy188III | TCNNGA | 5 cut(s) | 116, 442, 566, 740, 914 |
| Hpy8I | GTNNAC | 5 cut(s) | 265, 445, 493, 909, 955 |
| HpyAV | CCTTC | 3 cut(s) | 67, 110, 716 |
| HpyCH4III | ACNGT | 3 cut(s) | 294, 572, 1248 |
| HpyCH4IV | ACGT | 3 cut(s) | 327, 1164, 1207 |
| HpyCH4V | TGCA | 4 cut(s) | 165, 185, 507, 909 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 214 |
| HpyF3I | CTNAG | 1 cut(s) | 760 |
| HpySE526I | ACGT | 3 cut(s) | 327, 1164, 1207 |
| Hsp92II | CATG | 4 cut(s) | 49, 238, 524, 848 |
| Kpn2I | TCCGGA | 1 cut(s) | 565 |
| Kzo9I | GATC | 4 cut(s) | 130, 550, 725, 916 |
| LmnI | GCTCC | 2 cut(s) | 925, 994 |
| Lsp1109I | GCAGC | 4 cut(s) | 75, 78, 166, 169 |
| LweI | GCATC | 3 cut(s) | 78, 217, 534 |
| MaeI | CTAG | 3 cut(s) | 252, 711, 1264 |
| MaeII | ACGT | 3 cut(s) | 327, 1164, 1207 |
| MaeIII | GTNAC | 6 cut(s) | 52, 74, 254, 775, 1040, 1085 |
| MalI | GATC | 4 cut(s) | 132, 552, 727, 918 |
| MboI | GATC | 4 cut(s) | 130, 550, 725, 916 |
| MboII | GAAGA | 8 cut(s) | 51, 80, 137, 379, 442, 864, 870, 1165 |
| MflI | RGATCY | 1 cut(s) | 130 |
| MhlI | GDGCHC | 1 cut(s) | 911 |
| MluCI | AATT | 8 cut(s) | 190, 271, 805, 966, 982, 1059, 1093, 1177 |
| MlyI | GAGTC | 2 cut(s) | 637, 708 |
| MmeI | TCCRAC | 1 cut(s) | 108 |
| MnlI | CCTC | 7 cut(s) | 488, 492, 620, 670, 997, 1018, 1177 |
| MroI | TCCGGA | 1 cut(s) | 565 |
| MroXI | GAANNNNTTC | 1 cut(s) | 449 |
| MseI | TTAA | 1 cut(s) | 8 |
| MspI | CCGG | 1 cut(s) | 566 |
| MspR9I | CCNGG | 1 cut(s) | 921 |
| Mva1269I | GAATGC | 1 cut(s) | 612 |
| MvaI | CCWGG | 1 cut(s) | 921 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 214 |
| NdeII | GATC | 4 cut(s) | 130, 550, 725, 916 |
| NlaIII | CATG | 4 cut(s) | 49, 238, 524, 848 |
| NlaIV | GGNNCC | 3 cut(s) | 132, 468, 1224 |
| NmeAIII | GCCGAG | 1 cut(s) | 278 |
| NmuCI | GTSAC | 3 cut(s) | 254, 1040, 1085 |
| NspI | RCATGY | 1 cut(s) | 848 |
| NspV | TTCGAA | 1 cut(s) | 850 |
| PctI | GAATGC | 1 cut(s) | 612 |
| PdmI | GAANNNNTTC | 1 cut(s) | 449 |
| PfeI | GAWTC | 4 cut(s) | 112, 562, 667, 1115 |
| PflFI | GACNNNGTC | 1 cut(s) | 698 |
| PkrI | GCNGC | 4 cut(s) | 65, 68, 181, 184 |
| PleI | GAGTC | 2 cut(s) | 637, 707 |
| PpsI | GAGTC | 2 cut(s) | 637, 707 |
| PsiI | TTATAA | 1 cut(s) | 195 |
| Psp1406I | AACGTT | 1 cut(s) | 1207 |
| Psp6I | CCWGG | 1 cut(s) | 919 |
| PspFI | CCCAGC | 1 cut(s) | 14 |
| PspGI | CCWGG | 1 cut(s) | 919 |
| PspN4I | GGNNCC | 3 cut(s) | 132, 468, 1224 |
| PstNI | CAGNNNCTG | 1 cut(s) | 78 |
| PsuI | RGATCY | 1 cut(s) | 130 |
| PsyI | GACNNNGTC | 1 cut(s) | 698 |
| RsaI | GTAC | 5 cut(s) | 266, 296, 654, 932, 1192 |
| RsaNI | GTAC | 5 cut(s) | 265, 295, 653, 931, 1191 |
| SaqAI | TTAA | 1 cut(s) | 8 |
| SatI | GCNGC | 4 cut(s) | 64, 67, 180, 183 |
| Sau3AI | GATC | 4 cut(s) | 130, 550, 725, 916 |
| ScaI | AGTACT | 1 cut(s) | 932 |
| SchI | GAGTC | 2 cut(s) | 637, 708 |
| ScrFI | CCNGG | 1 cut(s) | 921 |
| SduI | GDGCHC | 1 cut(s) | 911 |
| SfaNI | GCATC | 3 cut(s) | 78, 217, 534 |
| SfcI | CTRYAG | 1 cut(s) | 767 |
| SfuI | TTCGAA | 1 cut(s) | 850 |
| Sse9I | AATT | 8 cut(s) | 190, 271, 805, 966, 982, 1059, 1093, 1177 |
| SsiI | CCGC | 2 cut(s) | 546, 1254 |
| SspI | AATATT | 1 cut(s) | 346 |
| SspMI | CTAG | 3 cut(s) | 252, 711, 1264 |
| StyD4I | CCNGG | 1 cut(s) | 919 |
| StyI | CCWWGG | 1 cut(s) | 825 |
| TaaI | ACNGT | 3 cut(s) | 294, 572, 1248 |
| TaiI | ACGT | 3 cut(s) | 330, 1167, 1210 |
| TaqI | TCGA | 5 cut(s) | 37, 219, 728, 850, 1211 |
| TaqII | GACCGA | 1 cut(s) | 711 |
| TasI | AATT | 8 cut(s) | 190, 271, 805, 966, 982, 1059, 1093, 1177 |
| TatI | WGTACW | 2 cut(s) | 294, 930 |
| TfiI | GAWTC | 4 cut(s) | 112, 562, 667, 1115 |
| Tru1I | TTAA | 1 cut(s) | 8 |
| Tru9I | TTAA | 1 cut(s) | 8 |
| TseFI | GTSAC | 3 cut(s) | 254, 1040, 1085 |
| TseI | GCWGC | 4 cut(s) | 63, 66, 179, 182 |
| Tsp45I | GTSAC | 3 cut(s) | 254, 1040, 1085 |
| TspDTI | ATGAA | 3 cut(s) | 380, 677, 735 |
| TspGWI | ACGGA | 1 cut(s) | 123 |
| Tth111I | GACNNNGTC | 1 cut(s) | 698 |
| VneI | GTGCAC | 1 cut(s) | 907 |
| XapI | RAATTY | 1 cut(s) | 1093 |
| XceI | RCATGY | 1 cut(s) | 848 |
| XmiI | GTMKAC | 2 cut(s) | 492, 954 |
| XmnI | GAANNNNTTC | 1 cut(s) | 449 |
| XspI | CTAG | 3 cut(s) | 252, 711, 1264 |
| ZrmI | AGTACT | 1 cut(s) | 932 |