Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 214 |
| Acc36I | ACCTGC | 1 cut(s) | 214 |
| AccB1I | GGYRCC | 1 cut(s) | 452 |
| AccI | GTMKAC | 1 cut(s) | 102 |
| AclWI | GGATC | 1 cut(s) | 7 |
| AcyI | GRCGYC | 1 cut(s) | 262 |
| AfaI | GTAC | 1 cut(s) | 142 |
| AfeI | AGCGCT | 1 cut(s) | 172 |
| AgsI | TTSAA | 1 cut(s) | 83 |
| AhlI | ACTAGT | 1 cut(s) | 137 |
| AluBI | AGCT | 2 cut(s) | 158, 322 |
| AluI | AGCT | 2 cut(s) | 158, 322 |
| Alw26I | GTCTC | 1 cut(s) | 77 |
| AlwI | GGATC | 1 cut(s) | 7 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 158 |
| Aor51HI | AGCGCT | 1 cut(s) | 172 |
| ApeKI | GCWGC | 3 cut(s) | 149, 152, 155 |
| AspLEI | GCGC | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 2 cut(s) | 245, 299 |
| BanI | GGYRCC | 1 cut(s) | 452 |
| BbvI | GCAGC | 3 cut(s) | 161, 164, 167 |
| BceAI | ACGGC | 1 cut(s) | 440 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 95, 129 |
| BclI | TGATCA | 1 cut(s) | 241 |
| BcoDI | GTCTC | 1 cut(s) | 77 |
| BcuI | ACTAGT | 1 cut(s) | 137 |
| BfaI | CTAG | 3 cut(s) | 138, 323, 460 |
| BfmI | CTRYAG | 2 cut(s) | 147, 279 |
| BfoI | RGCGCY | 1 cut(s) | 174 |
| BfuAI | ACCTGC | 1 cut(s) | 214 |
| BisI | GCNGC | 3 cut(s) | 150, 153, 156 |
| BlsI | GCNGC | 3 cut(s) | 151, 154, 157 |
| BmcAI | AGTACT | 1 cut(s) | 142 |
| BmiI | GGNNCC | 2 cut(s) | 416, 454 |
| BoxI | GACNNNNGTC | 1 cut(s) | 371 |
| BpmI | CTGGAG | 1 cut(s) | 102 |
| BsaHI | GRCGYC | 1 cut(s) | 262 |
| BsaWI | WCCGGW | 1 cut(s) | 405 |
| BseGI | GGATG | 1 cut(s) | 437 |
| BseMII | CTCAG | 1 cut(s) | 90 |
| BseRI | GAGGAG | 2 cut(s) | 82, 332 |
| BseXI | GCAGC | 3 cut(s) | 161, 164, 167 |
| BshNI | GGYRCC | 1 cut(s) | 452 |
| BsiSI | CCGG | 1 cut(s) | 406 |
| BsmAI | GTCTC | 1 cut(s) | 77 |
| Bsp143I | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| BspCNI | CTCAG | 1 cut(s) | 89 |
| BspLI | GGNNCC | 2 cut(s) | 416, 454 |
| BspMAI | CTGCAG | 1 cut(s) | 151 |
| BspMI | ACCTGC | 1 cut(s) | 214 |
| BspPI | GGATC | 1 cut(s) | 7 |
| BspT107I | GGYRCC | 1 cut(s) | 452 |
| BssMI | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| BssNI | GRCGYC | 1 cut(s) | 262 |
| BstACI | GRCGYC | 1 cut(s) | 262 |
| BstDEI | CTNAG | 1 cut(s) | 76 |
| BstF5I | GGATG | 1 cut(s) | 437 |
| BstH2I | RGCGCY | 1 cut(s) | 174 |
| BstHHI | GCGC | 1 cut(s) | 173 |
| BstKTI | GATC | 8 cut(s) | 15, 124, 228, 244, 292, 328, 382, 425 |
| BstMAI | GTCTC | 1 cut(s) | 77 |
| BstMBI | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 155 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 371 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 279 |
| BstV1I | GCAGC | 3 cut(s) | 161, 164, 167 |
| BtgZI | GCGATG | 1 cut(s) | 230 |
| BtsCI | GGATG | 1 cut(s) | 437 |
| BveI | ACCTGC | 1 cut(s) | 214 |
| CaiI | CAGNNNCTG | 1 cut(s) | 158 |
| CfoI | GCGC | 1 cut(s) | 173 |
| CseI | GACGC | 1 cut(s) | 270 |
| Csp6I | GTAC | 1 cut(s) | 141 |
| CviAII | CATG | 1 cut(s) | 164 |
| CviJI | RGCY | 3 cut(s) | 158, 322, 417 |
| CviKI_1 | RGCY | 3 cut(s) | 158, 322, 417 |
| CviQI | GTAC | 1 cut(s) | 141 |
| DdeI | CTNAG | 1 cut(s) | 76 |
| DpnI | GATC | 8 cut(s) | 14, 123, 227, 243, 291, 327, 381, 424 |
| DpnII | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| Eco47III | AGCGCT | 1 cut(s) | 172 |
| FaeI | CATG | 1 cut(s) | 167 |
| FaiI | YATR | 5 cut(s) | 47, 135, 165, 195, 420 |
| FatI | CATG | 1 cut(s) | 163 |
| FbaI | TGATCA | 1 cut(s) | 241 |
| FblI | GTMKAC | 1 cut(s) | 102 |
| Fnu4HI | GCNGC | 3 cut(s) | 150, 153, 156 |
| FokI | GGATG | 1 cut(s) | 424 |
| Fsp4HI | GCNGC | 3 cut(s) | 150, 153, 156 |
| FspBI | CTAG | 3 cut(s) | 138, 323, 460 |
| GlaI | GCGC | 1 cut(s) | 172 |
| GluI | GCNGC | 3 cut(s) | 150, 153, 156 |
| GsuI | CTGGAG | 1 cut(s) | 102 |
| HaeII | RGCGCY | 1 cut(s) | 174 |
| HapII | CCGG | 1 cut(s) | 406 |
| HgaI | GACGC | 1 cut(s) | 270 |
| HhaI | GCGC | 1 cut(s) | 173 |
| Hin1I | GRCGYC | 1 cut(s) | 262 |
| Hin1II | CATG | 1 cut(s) | 167 |
| Hin6I | GCGC | 1 cut(s) | 171 |
| HinP1I | GCGC | 1 cut(s) | 171 |
| HincII | GTYRAC | 2 cut(s) | 103, 386 |
| HindII | GTYRAC | 2 cut(s) | 103, 386 |
| HinfI | GANTC | 2 cut(s) | 70, 309 |
| HpaII | CCGG | 1 cut(s) | 406 |
| HphI | GGTGA | 2 cut(s) | 245, 299 |
| Hpy166II | GTNNAC | 2 cut(s) | 103, 386 |
| Hpy188I | TCNGA | 1 cut(s) | 12 |
| Hpy188III | TCNNGA | 1 cut(s) | 119 |
| Hpy8I | GTNNAC | 2 cut(s) | 103, 386 |
| Hpy99I | CGWCG | 5 cut(s) | 104, 107, 110, 113, 264 |
| HpyAV | CCTTC | 1 cut(s) | 260 |
| HpyCH4IV | ACGT | 1 cut(s) | 111 |
| HpyCH4V | TGCA | 4 cut(s) | 27, 149, 163, 444 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 155 |
| HpyF3I | CTNAG | 1 cut(s) | 76 |
| HpySE526I | ACGT | 1 cut(s) | 111 |
| Hsp92I | GRCGYC | 1 cut(s) | 262 |
| Hsp92II | CATG | 1 cut(s) | 167 |
| HspAI | GCGC | 1 cut(s) | 171 |
| Ksp22I | TGATCA | 1 cut(s) | 241 |
| Kzo9I | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| LmnI | GCTCC | 3 cut(s) | 121, 319, 414 |
| LpnPI | CCDG | 9 cut(s) | 49, 132, 203, 219, 233, 314, 419, 435, 454 |
| Lsp1109I | GCAGC | 3 cut(s) | 161, 164, 167 |
| MaeI | CTAG | 3 cut(s) | 138, 323, 460 |
| MaeII | ACGT | 1 cut(s) | 111 |
| MalI | GATC | 8 cut(s) | 14, 123, 227, 243, 291, 327, 381, 424 |
| MboI | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| MboII | GAAGA | 1 cut(s) | 88 |
| MluCI | AATT | 2 cut(s) | 184, 337 |
| MlyI | GAGTC | 1 cut(s) | 79 |
| MnlI | CCTC | 4 cut(s) | 33, 60, 287, 310 |
| MseI | TTAA | 2 cut(s) | 36, 213 |
| MslI | CAYNNNNRTG | 1 cut(s) | 22 |
| MspA1I | CMGCKG | 1 cut(s) | 158 |
| MspI | CCGG | 1 cut(s) | 406 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 155 |
| NdeII | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| NlaIII | CATG | 1 cut(s) | 167 |
| NlaIV | GGNNCC | 2 cut(s) | 416, 454 |
| PaqCI | CACCTGC | 1 cut(s) | 214 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 105, 108 |
| PfeI | GAWTC | 1 cut(s) | 309 |
| PkrI | GCNGC | 3 cut(s) | 151, 154, 157 |
| PleI | GAGTC | 1 cut(s) | 78 |
| PpsI | GAGTC | 1 cut(s) | 78 |
| PshAI | GACNNNNGTC | 1 cut(s) | 371 |
| PspN4I | GGNNCC | 2 cut(s) | 416, 454 |
| PstI | CTGCAG | 1 cut(s) | 151 |
| PstNI | CAGNNNCTG | 1 cut(s) | 158 |
| PvuII | CAGCTG | 1 cut(s) | 158 |
| RsaI | GTAC | 1 cut(s) | 142 |
| RsaNI | GTAC | 1 cut(s) | 141 |
| RseI | CAYNNNNRTG | 1 cut(s) | 22 |
| SalI | GTCGAC | 1 cut(s) | 101 |
| SaqAI | TTAA | 2 cut(s) | 36, 213 |
| SatI | GCNGC | 3 cut(s) | 150, 153, 156 |
| Sau3AI | GATC | 8 cut(s) | 12, 121, 225, 241, 289, 325, 379, 422 |
| ScaI | AGTACT | 1 cut(s) | 142 |
| SchI | GAGTC | 1 cut(s) | 79 |
| SetI | ASST | 6 cut(s) | 114, 160, 192, 208, 324, 375 |
| SfcI | CTRYAG | 2 cut(s) | 147, 279 |
| SgrDI | CGTCGACG | 1 cut(s) | 101 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 22 |
| SpeI | ACTAGT | 1 cut(s) | 137 |
| Sse9I | AATT | 2 cut(s) | 184, 337 |
| SspMI | CTAG | 3 cut(s) | 138, 323, 460 |
| TaiI | ACGT | 1 cut(s) | 114 |
| TaqI | TCGA | 2 cut(s) | 102, 292 |
| TasI | AATT | 2 cut(s) | 184, 337 |
| TatI | WGTACW | 1 cut(s) | 140 |
| TfiI | GAWTC | 1 cut(s) | 309 |
| Tru1I | TTAA | 2 cut(s) | 36, 213 |
| Tru9I | TTAA | 2 cut(s) | 36, 213 |
| TseI | GCWGC | 3 cut(s) | 149, 152, 155 |
| XmiI | GTMKAC | 1 cut(s) | 102 |
| XspI | CTAG | 3 cut(s) | 138, 323, 460 |
| ZrmI | AGTACT | 1 cut(s) | 142 |