FvH4_7g18050

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000390
Physical Location & Seq
Forward (+)
15175398 .. 15179007
3610 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18050.t1

Sequence Viewer

Length: 2226 bp
ATGGCTCTTCAATGGTTTCTCATTCTCAGTCTCATGAGCAGTATTCTGCTATTTGTTGACGTTACTCAGTCAACTTCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGGCAAGCCAAAGACTGGGGTGCTAGTAACACCTGATCTTCATGTGAAAATCCTACAAGATGTGGTTGACAGCAACAACCTTGATGTTGCTCATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGATGGAATGAATGGTGTAGTGTCTGTCTTCCCAAGCGAACGGAGGAGTGTCAAAACAACAAAGTCATGGAACTTCCTCGGATTTCCAGAAACAGTTAAGAGAAGTGCCCTCGAAAAGGATATCATCGTCGGAGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTCGGTCCTCCACCCAAAAGATGGAAAGGCAAATGTCAAGGAAATGGCGACTTTACTTGTAACAATAAAATTATTGGAGCACGTTACTACCGTGCTTCTGGAGATTTCGAAGGAGATTTTGAGTCTCCAAGAGATGCAGCCGGTCATGGTACCCACACTGCATCTACAGCAGCTGGGAATATAGTGAGCAAAGCTAGCCAAAATGGTTTAGGCTTGGGAACAGCAAGAGGAGGAGTGCCATCAGCACGTATTGCTGTGTACAAAGTATGCTGGAACGGCGAGACCGGTTGTGATGATGCTGACGTACTAGCAGCAATGGACGATGCCATTGCTGACGGTGTCGACATTCTCTCTATGTCCGTCGGAGGCAACATTCCATTTGATTATTTGAGAGATTCATTTGCAATTGCGACACTTCACGCTACCAGAGAAGGGATACTATGTTCCATGGCCGCTGGGAATGAAGGTCCGAAAGAAAAATCCGTGACGAACTTTGCACCATGGCAACTTTCTGTTGCTGCTTCCACCATAAACCGTCAGTTTATCACCAAGGTGCAATTGGGTAATGGAAAAATCTATGAGGGACTAATTTTTCCTAACCAAATTGACCTCAAGGGTAAGTTCTATCCTTTAGTTTATGGTGGAGATGCACCCAATAAAACATTAGGCAACGACACAGTCTCCGCCAGGTATTGTTGTGATGGCTGCTTAGACAAAGATTTGATCAAAGGCAAAATTGTGCTGTGTGATGGCCCTCTAGATGAAGCCGGGATTGGAGCTATATTGGGTGGCGCAGCCGGAGTTATTCTGACAAGCCAAAAGTTGCATGATGATTTGCTTGGCCCTGTTCCCGTGGCTGCATCTTATGTTGGATTGGAAGAAAATAGCAACATCTATAAATACATAAGCTCAACAAGGAACCCAACTGCAACTATATGGAAATGTGAAGAGATTGATGACGTATTGGCTCCATATGTGCCCTTCTACTCATCAAAGGGTCCAAATCCGATCAGTCCCAACATTCTCAAGCCAGATATAGCGGCTCCAGGAAGTTACATTCTAGCAGCATATCCTCCAACTAGTGTTGGCAATAGAGGTAGATACAATTTCTTAACAGGGTCATCAATGGCGTGCCCCCATGCGACGGCCGTAGCTGCATATGTCAAATCGTTTCACCCTAAATGGTCGCCTGCTGCTATTAGATCGGCTCTCATGACTACCGCCAAACCTATGAGTGCCAAAACTAGCCCTCTTGCTGAATTTGCATATGGAGCTGGCCTAATAAACCCATCTAGGGCTCCATATCCTGGTTTGGTTTACGATCTTGATGGACAAGACTACATAGCATTTTTGTGTTCACAAGGATACAGTGGCAAACTATTGCAAGCCATATCTGGGGAGAAGACTAGCTGCTCATCATCAAATTTTAATAACGGAACAGCCAGTGACCTAAACTATCCTTCTTTTACTCTTTCCATCAAGGATCCGAAATCCGTGAATGGTGTTTTTCATAGGACGGTCACGAATGTTGGATCGTGGAATTCAACATATAAAGCTAAAGTGGTAGCTCCATCAGGACTCAAAGTAAGTGTGATCCCAAGTGTTCTATCATTCACAGCTCTTGGACAGAAGAAGTCTTTTGTGGTCACAATAAAAGGACCAATGGAGAAATCAAATATAGTCTCTGCGTCTTTGGTATGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATCGTCGTCTATGTTGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

742

Amino Acids

78.99

Weight (kDa)

7.82

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 9e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 583 2.5e-44 Subtilase family
fn3_6 PF17766 640 - 737 9.6e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 620
AccB7I CCANNNNNTGG 3 cut(s) 128, 492, 1778
AccI GTMKAC 1 cut(s) 813
AciI CCGC 6 cut(s) 114, 924, 1155, 1511, 1692, 2220
AclWI GGATC 4 cut(s) 1949, 1962, 2011, 2059
AcoI YGGCCR 3 cut(s) 440, 921, 1617
AcsI RAATTY 4 cut(s) 435, 1730, 1894, 2011
AfaI GTAC 3 cut(s) 622, 731, 777
AfiI CCNNNNNNNGG 8 cut(s) 128, 403, 492, 903, 1615, 1765, 1778, 1866
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 3 cut(s) 11, 244, 2016
AhlI ACTAGT 1 cut(s) 1550
AjnI CCWGG 3 cut(s) 1157, 1516, 1777
AjuI GAANNNNNNNTTGG 2 cut(s) 1227, 1259
AleI CACNNNNGTG 1 cut(s) 1022
AloI GAACNNNNNNTCC 2 cut(s) 898, 930
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 5 cut(s) 35, 600, 746, 1156, 2158
AlwI GGATC 4 cut(s) 1949, 1962, 2011, 2059
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 6 cut(s) 440, 921, 1222, 1312, 1617, 1747
ApoI RAATTY 4 cut(s) 435, 1730, 1894, 2011
AsiGI ACCGGT 1 cut(s) 755
Asp718I GGTACC 1 cut(s) 620
AspLEI GCGC 1 cut(s) 1265
AspS9I GGNCC 6 cut(s) 476, 938, 1223, 1313, 1469, 2129
AsuC2I CCSGG 1 cut(s) 1240
AsuHPI GGTGA 2 cut(s) 1009, 1637
AsuII TTCGAA 1 cut(s) 579
AsuNHI GCTAGC 1 cut(s) 665
AvaII GGWCC 4 cut(s) 476, 938, 1469, 2129
BaeGI GKGCMC 3 cut(s) 397, 1452, 1607
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1954
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 2 cut(s) 1771, 2201
BbsI GAAGAC 2 cut(s) 307, 1880
Bbv12I GWGCWC 1 cut(s) 553
BceAI ACGGC 3 cut(s) 763, 1604, 1632
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 3 cut(s) 1159, 1518, 1779
BciVI GTATCC 2 cut(s) 900, 1829
BclI TGATCA 1 cut(s) 1194
BcnI CCSGG 1 cut(s) 1240
BcoDI GTCTC 5 cut(s) 35, 600, 746, 1156, 2158
BcuI ACTAGT 1 cut(s) 1550
BfmI CTRYAG 1 cut(s) 636
BfuI GTATCC 2 cut(s) 900, 1829
BglI GCCNNNNNGGC 1 cut(s) 92
Bme1390I CCNGG 4 cut(s) 1159, 1240, 1518, 1779
Bme18I GGWCC 4 cut(s) 476, 938, 1469, 2129
BmgT120I GGNCC 6 cut(s) 476, 938, 1223, 1313, 1469, 2129
BmiI GGNNCC 8 cut(s) 622, 1391, 1440, 1470, 1515, 1770, 1956, 2198
BmrFI CCNGG 4 cut(s) 1159, 1240, 1518, 1779
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 8 cut(s) 73, 454, 595, 641, 757, 784, 1108, 1340
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 138
BpiI GAAGAC 2 cut(s) 307, 1880
BpmI CTGGAG 2 cut(s) 591, 1500
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 2 cut(s) 1067, 1481
BpuMI CCSGG 1 cut(s) 1240
BsaAI YACGTR 1 cut(s) 719
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 5 cut(s) 364, 918, 971, 1020, 1323
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 2 cut(s) 1136, 1166
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 403, 492, 903, 1615, 1765, 1778, 1866
Bse118I RCCGGY 2 cut(s) 611, 755
Bse1I ACTGG 2 cut(s) 133, 1914
Bse3DI GCAATG 3 cut(s) 264, 792, 798
BseBI CCWGG 3 cut(s) 1159, 1518, 1779
BseDI CCNNGG 5 cut(s) 364, 918, 971, 1020, 1323
BseGI GGATG 2 cut(s) 88, 2179
BseLI CCNNNNNNNGG 8 cut(s) 128, 403, 492, 903, 1615, 1765, 1778, 1866
BseMI GCAATG 3 cut(s) 264, 792, 798
BseMII CTCAG 3 cut(s) 40, 80, 92
BseNI ACTGG 2 cut(s) 133, 1914
BseRI GAGGAG 3 cut(s) 346, 714, 717
BseSI GKGCMC 3 cut(s) 397, 1452, 1607
BseX3I CGGCCG 1 cut(s) 1617
BseYI CCCAGC 2 cut(s) 644, 926
Bsh1285I CGRYCG 1 cut(s) 1620
BshFI GGCC 6 cut(s) 442, 923, 1224, 1314, 1619, 1749
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 1 cut(s) 755
BsiEI CGRYCG 1 cut(s) 1620
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 4 cut(s) 612, 756, 1239, 1269
BslFI GGGAC 2 cut(s) 1068, 1470
BslI CCNNNNNNNGG 8 cut(s) 128, 403, 492, 903, 1615, 1765, 1778, 1866
BsmAI GTCTC 5 cut(s) 35, 600, 746, 1156, 2158
BsmFI GGGAC 2 cut(s) 1068, 1470
BsnI GGCC 6 cut(s) 442, 923, 1224, 1314, 1619, 1749
Bso31I GGTCTC 1 cut(s) 746
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 6 cut(s) 397, 553, 1452, 1607, 1771, 2201
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
Bsp19I CCATGG 2 cut(s) 918, 971
BspACI CCGC 6 cut(s) 114, 924, 1155, 1511, 1692, 2220
BspANI GGCC 6 cut(s) 442, 923, 1224, 1314, 1619, 1749
BspCNI CTCAG 3 cut(s) 39, 79, 91
BspHI TCATGA 3 cut(s) 33, 205, 1683
BspLI GGNNCC 8 cut(s) 622, 1391, 1440, 1470, 1515, 1770, 1956, 2198
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 4 cut(s) 1949, 1962, 2011, 2059
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 620
BspTNI GGTCTC 1 cut(s) 746
BsrDI GCAATG 3 cut(s) 264, 792, 798
BsrFI RCCGGY 2 cut(s) 611, 755
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 2 cut(s) 133, 1914
BssAI RCCGGY 2 cut(s) 611, 755
BssECI CCNNGG 5 cut(s) 364, 918, 971, 1020, 1323
BssMI GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
BssT1I CCWWGG 3 cut(s) 918, 971, 1020
Bst2UI CCWGG 3 cut(s) 1159, 1518, 1779
Bst4CI ACNGT 8 cut(s) 382, 431, 563, 809, 1007, 1150, 1841, 1990
Bst6I CTCTTC 2 cut(s) 12, 1413
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 119, 667, 1603, 1662, 1747, 1857
BstDEI CTNAG 5 cut(s) 26, 66, 78, 1180, 2223
BstDSI CCRYGG 3 cut(s) 918, 971, 1323
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 2 cut(s) 88, 2179
BstHHI GCGC 1 cut(s) 1265
BstKTI GATC 8 cut(s) 151, 1197, 1482, 1676, 1795, 1957, 2006, 2067
BstMAI GTCTC 5 cut(s) 35, 600, 746, 1156, 2158
BstMBI GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
BstMCI CGRYCG 1 cut(s) 1620
BstMWI GCNNNNNNNGC 9 cut(s) 92, 209, 638, 666, 722, 747, 1625, 1733, 1742
BstNI CCWGG 3 cut(s) 1159, 1518, 1779
BstSCI CCNGG 4 cut(s) 1157, 1238, 1516, 1777
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 3 cut(s) 397, 1452, 1607
BstV2I GAAGAC 2 cut(s) 307, 1880
BstX2I RGATCY 1 cut(s) 1954
BstYI RGATCY 1 cut(s) 1954
BstZI CGGCCG 1 cut(s) 1617
BsuI GTATCC 2 cut(s) 900, 1829
BsuRI GGCC 6 cut(s) 442, 923, 1224, 1314, 1619, 1749
BtgI CCRYGG 3 cut(s) 918, 971, 1323
BtsCI GGATG 2 cut(s) 88, 2179
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 4 cut(s) 436, 627, 1846, 1921
Cac8I GCNNGC 6 cut(s) 119, 667, 1603, 1662, 1747, 1857
CaiI CAGNNNCTG 1 cut(s) 644
CciI TCATGA 3 cut(s) 33, 205, 1683
CfoI GCGC 1 cut(s) 1265
Cfr10I RCCGGY 2 cut(s) 611, 755
Cfr13I GGNCC 6 cut(s) 476, 938, 1223, 1313, 1469, 2129
CseI GACGC 1 cut(s) 2148
Csp6I GTAC 3 cut(s) 621, 730, 776
CspAI ACCGGT 1 cut(s) 755
CviQI GTAC 3 cut(s) 621, 730, 776
DdeI CTNAG 5 cut(s) 26, 66, 78, 1180, 2223
DpnI GATC 8 cut(s) 150, 1196, 1481, 1675, 1794, 1956, 2005, 2066
DpnII GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
EaeI YGGCCR 3 cut(s) 440, 921, 1617
EagI CGGCCG 1 cut(s) 1617
Eam1104I CTCTTC 2 cut(s) 12, 1413
EarI CTCTTC 2 cut(s) 12, 1413
EciI GGCGGA 1 cut(s) 1144
EclXI CGGCCG 1 cut(s) 1617
Eco130I CCWWGG 3 cut(s) 918, 971, 1020
Eco24I GRGCYC 2 cut(s) 1771, 2201
Eco31I GGTCTC 1 cut(s) 746
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 476, 938, 1469, 2129
Eco52I CGGCCG 1 cut(s) 1617
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 2011
EcoRII CCWGG 3 cut(s) 1157, 1516, 1777
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 3 cut(s) 918, 971, 1020
EcoT38I GRGCYC 2 cut(s) 1771, 2201
ErhI CCWWGG 3 cut(s) 918, 971, 1020
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 2 cut(s) 1068, 1470
FauNDI CATATG 3 cut(s) 1444, 1630, 1738
FbaI TGATCA 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 813
FokI GGATG 2 cut(s) 95, 2186
FriOI GRGCYC 2 cut(s) 1771, 2201
GlaI GCGC 1 cut(s) 1264
GsaI CCCAGC 2 cut(s) 648, 930
GsuI CTGGAG 2 cut(s) 591, 1500
HaeIII GGCC 6 cut(s) 442, 923, 1224, 1314, 1619, 1749
HapII CCGG 4 cut(s) 612, 756, 1239, 1269
HgaI GACGC 1 cut(s) 2148
HhaI GCGC 1 cut(s) 1265
Hin6I GCGC 1 cut(s) 1263
HinP1I GCGC 1 cut(s) 1263
HincII GTYRAC 4 cut(s) 58, 72, 181, 814
HindII GTYRAC 4 cut(s) 58, 72, 181, 814
HinfI GANTC 4 cut(s) 446, 593, 866, 2049
HpaII CCGG 4 cut(s) 612, 756, 1239, 1269
HphI GGTGA 2 cut(s) 1009, 1637
Hpy166II GTNNAC 7 cut(s) 58, 72, 181, 730, 814, 1789, 1829
Hpy188I TCNGA 8 cut(s) 368, 419, 451, 836, 942, 1281, 1479, 1959
Hpy8I GTNNAC 7 cut(s) 58, 72, 181, 730, 814, 1789, 1829
Hpy99I CGWCG 4 cut(s) 419, 836, 1618, 2210
HpyAV CCTTC 5 cut(s) 575, 896, 929, 1462, 1941
HpyCH4III ACNGT 8 cut(s) 382, 431, 563, 809, 1007, 1150, 1841, 1990
HpyCH4IV ACGT 5 cut(s) 60, 553, 718, 774, 1431
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 209, 638, 666, 722, 747, 1625, 1733, 1742
HpyF3I CTNAG 5 cut(s) 26, 66, 78, 1180, 2223
HpySE526I ACGT 5 cut(s) 60, 553, 718, 774, 1431
HspAI GCGC 1 cut(s) 1263
KpnI GGTACC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 1194
Kzo9I GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 8 cut(s) 548, 1247, 1444, 1519, 1742, 1774, 2044, 2196
LweI GCATC 8 cut(s) 73, 454, 595, 641, 757, 784, 1108, 1340
MaeII ACGT 5 cut(s) 60, 553, 718, 774, 1431
MaeIII GTNAC 9 cut(s) 61, 139, 530, 554, 955, 1523, 1916, 1990, 2116
MalI GATC 8 cut(s) 150, 1196, 1481, 1675, 1794, 1956, 2005, 2066
MboI GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
MboII GAAGA 7 cut(s) 143, 212, 307, 1361, 1430, 1885, 2113
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 1 cut(s) 1954
MhlI GDGCHC 6 cut(s) 397, 553, 1452, 1607, 1771, 2201
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 3 cut(s) 455, 602, 2043
MmeI TCCRAC 6 cut(s) 397, 429, 814, 1321, 1571, 1981
Mox20I TGGCCA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 442
MseI TTAA 3 cut(s) 384, 1583, 1899
MslI CAYNNNNRTG 2 cut(s) 1022, 1822
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 644, 926
MspI CCGG 4 cut(s) 612, 756, 1239, 1269
MspR9I CCNGG 4 cut(s) 1159, 1240, 1518, 1779
MunI CAATTG 2 cut(s) 876, 1028
MvaI CCWGG 3 cut(s) 1159, 1518, 1779
MwoI GCNNNNNNNGC 9 cut(s) 92, 209, 638, 666, 722, 747, 1625, 1733, 1742
NciI CCSGG 1 cut(s) 1240
NcoI CCATGG 2 cut(s) 918, 971
NdeI CATATG 3 cut(s) 1444, 1630, 1738
NdeII GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
NheI GCTAGC 1 cut(s) 665
NlaIV GGNNCC 8 cut(s) 622, 1391, 1440, 1470, 1515, 1770, 1956, 2198
NmuCI GTSAC 4 cut(s) 955, 1916, 1990, 2116
NspV TTCGAA 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 1022
PagI TCATGA 3 cut(s) 33, 205, 1683
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 559
PfeI GAWTC 1 cut(s) 866
PflFI GACNNNGTC 2 cut(s) 809, 1148
PflMI CCANNNNNTGG 3 cut(s) 128, 492, 1778
PfoI TCCNGGA 1 cut(s) 1516
PinAI ACCGGT 1 cut(s) 755
PleI GAGTC 3 cut(s) 454, 601, 2043
PpsI GAGTC 3 cut(s) 454, 601, 2043
Ppu21I YACGTR 1 cut(s) 719
Psp6I CCWGG 3 cut(s) 1157, 1516, 1777
PspFI CCCAGC 2 cut(s) 644, 926
PspGI CCWGG 3 cut(s) 1157, 1516, 1777
PspN4I GGNNCC 8 cut(s) 622, 1391, 1440, 1470, 1515, 1770, 1956, 2198
PspPI GGNCC 6 cut(s) 476, 938, 1223, 1313, 1469, 2129
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 1954
PsyI GACNNNGTC 2 cut(s) 809, 1148
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 3 cut(s) 622, 731, 777
RsaNI GTAC 3 cut(s) 621, 730, 776
RseI CAYNNNNRTG 2 cut(s) 1022, 1822
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 384, 1583, 1899
Sau3AI GATC 8 cut(s) 148, 1194, 1479, 1673, 1792, 1954, 2003, 2064
Sau96I GGNCC 6 cut(s) 476, 938, 1223, 1313, 1469, 2129
SchI GAGTC 3 cut(s) 455, 602, 2043
ScrFI CCNGG 4 cut(s) 1159, 1240, 1518, 1779
SduI GDGCHC 6 cut(s) 397, 553, 1452, 1607, 1771, 2201
SfaNI GCATC 8 cut(s) 73, 454, 595, 641, 757, 784, 1108, 1340
SfcI CTRYAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 476, 938, 1469, 2129
SmiMI CAYNNNNRTG 2 cut(s) 1022, 1822
SmlI CTYRAG 2 cut(s) 1082, 1496
SmoI CTYRAG 2 cut(s) 1082, 1496
SpeI ACTAGT 1 cut(s) 1550
SsiI CCGC 6 cut(s) 114, 924, 1155, 1511, 1692, 2220
StyD4I CCNGG 4 cut(s) 1157, 1238, 1516, 1777
StyI CCWWGG 3 cut(s) 918, 971, 1020
TaaI ACNGT 8 cut(s) 382, 431, 563, 809, 1007, 1150, 1841, 1990
TaiI ACGT 5 cut(s) 63, 556, 721, 777, 1434
TaqI TCGA 3 cut(s) 399, 579, 813
TaqII GACCGA 1 cut(s) 464
TatI WGTACW 1 cut(s) 729
TauI GCSGC 4 cut(s) 117, 926, 1514, 2222
TfiI GAWTC 1 cut(s) 866
Tru1I TTAA 3 cut(s) 384, 1583, 1899
Tru9I TTAA 3 cut(s) 384, 1583, 1899
TscAI CASTG 4 cut(s) 436, 634, 1846, 1921
TseFI GTSAC 4 cut(s) 955, 1916, 1990, 2116
Tsp45I GTSAC 4 cut(s) 955, 1916, 1990, 2116
TspDTI ATGAA 7 cut(s) 143, 222, 311, 858, 948, 1248, 1970
TspGWI ACGGA 5 cut(s) 343, 820, 943, 1920, 1954
TspRI CASTG 4 cut(s) 436, 634, 1846, 1921
Tth111I GACNNNGTC 2 cut(s) 809, 1148
Van91I CCANNNNNTGG 3 cut(s) 128, 492, 1778
VpaK11BI GGWCC 4 cut(s) 476, 938, 1469, 2129
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 4 cut(s) 435, 1730, 1894, 2011
XbaI TCTAGA 1 cut(s) 1228
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.