RchiOBHm_Chr1g0369661

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Forward (+)
59398166 .. 59402737
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59389

Sequence Viewer

Length: 2211 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAATATTCTACTGCTTGTTGATGTTACTCACTCAGTTGCTCAGGATGCCCGAAAGACTTATGTTGTGTATATGGGCGACAAGCCAAAAAACGGGCCCATATTACCTTATCTTCACGTGAATATGCTACAAGACGTAGTTGACAGCAGCAACATTGATATTGCAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTTAATGGATTTGCAGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAAGGAGTGCCAAAACAACAAAGTCATGGAACTTTCTTGGATTTCCAGAAACAGTAAAGAGAAGCGCCCTTGAAAGGGATATCATTGTAGGTGTGATGGACAGTGGAATTTGGCCAGAGTCAAACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCCATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAGTATTACCGTGCTTCTCGATCCTTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAGCGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGAGTAAGGCAAGCCTAAATGGTTTAGGGCTGGGAACAGCAAGAGGAGGGGTACCATCAGCACGTATTGCTGTGTACAAAATATGTTGGGGCAGCAGCATCAGTTGTGATGATGCTGACATTCTAGCGGCATTCGATGATGCCATTGCTGACGGTGTTGACATTCTCTCTATTTCCGTTGGAGGCCTTATTCCATCTGATTATTTTAATGATCCATTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAATGGTAAGCTCTATGAGGGACTCATACCAAACATATATGACCTCCGGAGTGAATTCTATCCTTTAATTTATGGTGGAGATGCACCCAATAAAAAAGCAGGTGTTGATGGGACTATCTCCAGGTTTTGTGTAAGAGATGCCTTAGAAGAAGATTTGATTAAAGGTAAAATTGTGCTTTGTGATGGCCCTATCGACGGGGCTGGGGTTGGGGCCATATTGGGTGGTGCAGCTGGAATTGTTCTGACAGGAAAAAAAGTTGCTGATGAAGCGCTTGGCCCTTATCTCGTGCCTGCATCTTACGTCGGATTGGAAGAAAACAGCGACATTTACAAATACATAAACTCAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATATGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAAAGCTAGATACTATCTAGAAACTGGGACATCAATGGCATGTCCCCATGCTACAGCCGTAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCACTTATCACTACTGCTAAACCTATGAGTGCCAAAACTAGCTCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAAGGCGCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAAGCATAACCAGGGACAAGGCTAGTTGCTCATCAAAATCTATTAATGGAACAACTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATACGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATATAGAGCTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACAACTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAATGGGCCGATTGAGAAATCAAATCTAGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

736

Amino Acids

78.65

Weight (kDa)

8.42

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 109 1.1e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 132 - 578 2.7e-42 Subtilase family
fn3_6 PF17766 635 - 732 1.4e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1115
Acc36I ACCTGC 1 cut(s) 1115
Acc65I GGTACC 1 cut(s) 697
AccB1I GGYRCC 2 cut(s) 697, 1756
AccB7I CCANNNNNTGG 3 cut(s) 483, 1541, 1766
AccIII TCCGGA 1 cut(s) 1071
AciI CCGC 6 cut(s) 603, 621, 773, 915, 997, 1499
AclWI GGATC 3 cut(s) 558, 851, 1996
AcoI YGGCCR 2 cut(s) 431, 912
AcsI RAATTY 4 cut(s) 426, 1079, 1718, 1946
AcvI CACGTG 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 1757
AfaI GTAC 3 cut(s) 699, 722, 1778
AfeI AGCGCT 1 cut(s) 1296
AgsI TTSAA 3 cut(s) 392, 571, 1019
AjnI CCWGG 4 cut(s) 1145, 1504, 1765, 1853
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 3 cut(s) 588, 591, 2143
AlwI GGATC 3 cut(s) 558, 851, 1996
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 1 cut(s) 2078
Aor13HI TCCGGA 1 cut(s) 1071
Aor51HI AGCGCT 1 cut(s) 1296
ApaI GGGCCC 2 cut(s) 135, 1460
ApoI RAATTY 4 cut(s) 426, 1079, 1718, 1946
ArsI GACNNNNNNTTYG 2 cut(s) 1703, 1735
AseI ATTAAT 1 cut(s) 1887
Asp718I GGTACC 1 cut(s) 697
AspLEI GCGC 3 cut(s) 386, 1297, 1759
AsuC2I CCSGG 1 cut(s) 933
AsuHPI GGTGA 3 cut(s) 1000, 1625, 1638
AvaI CYCGRG 1 cut(s) 2078
AvaII GGWCC 2 cut(s) 467, 929
BaeGI GKGCMC 3 cut(s) 135, 1440, 1460
BaeI ACNNNNGTAYC 2 cut(s) 1816, 1849
BalI TGGCCA 1 cut(s) 433
BanI GGYRCC 2 cut(s) 697, 1756
BanII GRGCYC 3 cut(s) 135, 1460, 2186
BauI CACGAG 1 cut(s) 1310
BbrPI CACGTG 1 cut(s) 154
BbsI GAAGAC 2 cut(s) 298, 1954
Bbv12I GWGCWC 1 cut(s) 544
BceAI ACGGC 1 cut(s) 1592
BciT130I CCWGG 4 cut(s) 1147, 1506, 1767, 1855
BciVI GTATCC 2 cut(s) 1817, 1933
BcnI CCSGG 1 cut(s) 933
BcoDI GTCTC 3 cut(s) 588, 591, 2143
BfaI CTAG 6 cut(s) 770, 1557, 1568, 1704, 1866, 2135
BfmI CTRYAG 1 cut(s) 1602
BfoI RGCGCY 3 cut(s) 387, 1298, 1760
BfuAI ACCTGC 1 cut(s) 1115
BfuI GTATCC 2 cut(s) 1817, 1933
BglII AGATCT 1 cut(s) 202
Bme1390I CCNGG 5 cut(s) 933, 1147, 1506, 1767, 1855
Bme18I GGWCC 2 cut(s) 467, 929
BmeT110I CYCGRG 1 cut(s) 2078
BmrFI CCNGG 5 cut(s) 933, 1147, 1506, 1767, 1855
BmrI ACTGGG 1 cut(s) 1584
BmsI GCATC 8 cut(s) 73, 632, 748, 753, 775, 1096, 1153, 1328
BmuI ACTGGG 1 cut(s) 1584
BpiI GAAGAC 2 cut(s) 298, 1954
BpmI CTGGAG 2 cut(s) 1129, 1488
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 583, 1469
BpuMI CCSGG 1 cut(s) 933
BsaAI YACGTR 2 cut(s) 154, 710
BsaHI GRCGYC 1 cut(s) 1757
BsaJI CCNNGG 5 cut(s) 505, 909, 962, 1011, 1854
BsaWI WCCGGW 1 cut(s) 1071
Bse118I RCCGGY 1 cut(s) 1648
Bse1I ACTGG 1 cut(s) 1579
Bse3DI GCAATG 2 cut(s) 255, 789
BseAI TCCGGA 1 cut(s) 1071
BseBI CCWGG 4 cut(s) 1147, 1506, 1767, 1855
BseDI CCNNGG 5 cut(s) 505, 909, 962, 1011, 1854
BseGI GGATG 3 cut(s) 88, 295, 2164
BseMI GCAATG 2 cut(s) 255, 789
BseMII CTCAG 2 cut(s) 84, 92
BseNI ACTGG 1 cut(s) 1579
BseRI GAGGAG 1 cut(s) 705
BseSI GKGCMC 3 cut(s) 135, 1440, 1460
BseYI CCCAGC 3 cut(s) 635, 676, 1226
BsgI GTGCAG 1 cut(s) 1272
BshNI GGYRCC 2 cut(s) 697, 1756
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 2078
BsiSI CCGG 3 cut(s) 932, 1072, 1649
BslFI GGGAC 6 cut(s) 1059, 1150, 1578, 1591, 1871, 2046
BsmAI GTCTC 3 cut(s) 588, 591, 2143
BsmFI GGGAC 6 cut(s) 1059, 1150, 1578, 1591, 1871, 2046
BsmI GAATGC 1 cut(s) 776
BsoBI CYCGRG 1 cut(s) 2078
Bsp120I GGGCCC 2 cut(s) 131, 1456
Bsp1286I GDGCHC 5 cut(s) 135, 544, 1440, 1460, 2186
Bsp13I TCCGGA 1 cut(s) 1071
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
Bsp19I CCATGG 3 cut(s) 505, 909, 962
BspACI CCGC 6 cut(s) 603, 621, 773, 915, 997, 1499
BspCNI CTCAG 2 cut(s) 83, 91
BspEI TCCGGA 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 1115
BspPI GGATC 3 cut(s) 558, 851, 1996
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 697, 1756
BsrDI GCAATG 2 cut(s) 255, 789
BsrFI RCCGGY 1 cut(s) 1648
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 1 cut(s) 1579
BssAI RCCGGY 1 cut(s) 1648
BssECI CCNNGG 5 cut(s) 505, 909, 962, 1011, 1854
BssMI GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
BssNI GRCGYC 1 cut(s) 1757
BssSI CACGAG 1 cut(s) 1310
BssT1I CCWWGG 4 cut(s) 505, 909, 962, 1011
Bst2BI CACGAG 1 cut(s) 1310
Bst2UI CCWGG 4 cut(s) 1147, 1506, 1767, 1855
Bst4CI ACNGT 9 cut(s) 373, 422, 546, 554, 646, 800, 946, 1829, 1975
Bst6I CTCTTC 2 cut(s) 12, 1401
BstACI GRCGYC 1 cut(s) 1757
BstAPI GCANNNNNTGC 1 cut(s) 713
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 2 cut(s) 154, 710
BstC8I GCNNGC 3 cut(s) 658, 1317, 1735
BstDEI CTNAG 4 cut(s) 70, 78, 1168, 1752
BstDSI CCRYGG 3 cut(s) 505, 909, 962
BstEII GGTNACC 1 cut(s) 1644
BstF5I GGATG 3 cut(s) 88, 295, 2164
BstH2I RGCGCY 3 cut(s) 387, 1298, 1760
BstHHI GCGC 3 cut(s) 386, 1297, 1759
BstKTI GATC 6 cut(s) 205, 566, 859, 1470, 1783, 1991
BstMAI GTCTC 3 cut(s) 588, 591, 2143
BstMBI GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
BstMWI GCNNNNNNNGC 8 cut(s) 83, 259, 629, 713, 1292, 1613, 1721, 1730
BstNI CCWGG 4 cut(s) 1147, 1506, 1767, 1855
BstNSI RCATGY 1 cut(s) 1593
BstPI GGTNACC 1 cut(s) 1644
BstSCI CCNGG 5 cut(s) 931, 1145, 1504, 1765, 1853
BstSFI CTRYAG 1 cut(s) 1602
BstSLI GKGCMC 3 cut(s) 135, 1440, 1460
BstV2I GAAGAC 2 cut(s) 298, 1954
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuI GTATCC 2 cut(s) 1817, 1933
BtgI CCRYGG 3 cut(s) 505, 909, 962
BtsCI GGATG 3 cut(s) 88, 295, 2164
BtsIMutI CAGTG 3 cut(s) 427, 651, 1834
BveI ACCTGC 1 cut(s) 1115
Cac8I GCNNGC 3 cut(s) 658, 1317, 1735
CaiI CAGNNNCTG 1 cut(s) 635
CfoI GCGC 3 cut(s) 386, 1297, 1759
Cfr10I RCCGGY 1 cut(s) 1648
CseI GACGC 2 cut(s) 1721, 2133
Csp6I GTAC 3 cut(s) 698, 721, 1777
CviAII CATG 8 cut(s) 345, 506, 608, 910, 963, 1590, 1598, 1906
CviQI GTAC 3 cut(s) 698, 721, 1777
DdeI CTNAG 4 cut(s) 70, 78, 1168, 1752
DinI GGCGCC 1 cut(s) 1758
DpnI GATC 6 cut(s) 204, 565, 858, 1469, 1782, 1990
DpnII GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
EaeI YGGCCR 2 cut(s) 431, 912
Eam1104I CTCTTC 2 cut(s) 12, 1401
EarI CTCTTC 2 cut(s) 12, 1401
Eco130I CCWWGG 4 cut(s) 505, 909, 962, 1011
Eco147I AGGCCT 1 cut(s) 831
Eco24I GRGCYC 3 cut(s) 135, 1460, 2186
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 2 cut(s) 467, 929
Eco47III AGCGCT 1 cut(s) 1296
Eco72I CACGTG 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 2078
Eco91I GGTNACC 1 cut(s) 1644
EcoO109I RGGNCCY 1 cut(s) 1456
EcoO65I GGTNACC 1 cut(s) 1644
EcoRI GAATTC 1 cut(s) 1079
EcoRII CCWGG 4 cut(s) 1145, 1504, 1765, 1853
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 4 cut(s) 505, 909, 962, 1011
EcoT38I GRGCYC 3 cut(s) 135, 1460, 2186
EgeI GGCGCC 1 cut(s) 1758
EheI GGCGCC 1 cut(s) 1758
ErhI CCWWGG 4 cut(s) 505, 909, 962, 1011
FaeI CATG 8 cut(s) 348, 509, 611, 913, 966, 1593, 1601, 1909
FaqI GGGAC 6 cut(s) 1059, 1150, 1578, 1591, 1871, 2046
FatI CATG 8 cut(s) 344, 505, 607, 909, 962, 1589, 1597, 1905
FauNDI CATATG 2 cut(s) 1432, 1618
FokI GGATG 3 cut(s) 95, 302, 2171
FriOI GRGCYC 3 cut(s) 135, 1460, 2186
FspBI CTAG 6 cut(s) 770, 1557, 1568, 1704, 1866, 2135
GlaI GCGC 3 cut(s) 385, 1296, 1758
GsaI CCCAGC 3 cut(s) 639, 680, 1230
GsuI CTGGAG 2 cut(s) 1129, 1488
HaeII RGCGCY 3 cut(s) 387, 1298, 1760
HapII CCGG 3 cut(s) 932, 1072, 1649
HgaI GACGC 2 cut(s) 1721, 2133
HhaI GCGC 3 cut(s) 386, 1297, 1759
Hin1I GRCGYC 1 cut(s) 1757
Hin1II CATG 8 cut(s) 348, 509, 611, 913, 966, 1593, 1601, 1909
Hin6I GCGC 3 cut(s) 384, 1295, 1757
HinP1I GCGC 3 cut(s) 384, 1295, 1757
HincII GTYRAC 2 cut(s) 178, 805
HindII GTYRAC 2 cut(s) 178, 805
HinfI GANTC 6 cut(s) 437, 584, 596, 1047, 2034, 2050
HpaII CCGG 3 cut(s) 932, 1072, 1649
HphI GGTGA 3 cut(s) 1000, 1625, 1638
Hpy166II GTNNAC 7 cut(s) 178, 721, 805, 1777, 1817, 1835, 2070
Hpy188I TCNGA 6 cut(s) 539, 844, 1269, 1331, 1467, 1711
Hpy188III TCNNGA 7 cut(s) 80, 365, 561, 1072, 1568, 1784, 2179
Hpy8I GTNNAC 7 cut(s) 178, 721, 805, 1777, 1817, 1835, 2070
Hpy99I CGWCG 2 cut(s) 1223, 1331
HpyAV CCTTC 5 cut(s) 32, 577, 920, 1758, 1926
HpyCH4III ACNGT 9 cut(s) 373, 422, 546, 554, 646, 800, 946, 1829, 1975
HpyCH4IV ACGT 4 cut(s) 153, 171, 709, 1326
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 259, 629, 713, 1292, 1613, 1721, 1730
HpyF3I CTNAG 4 cut(s) 70, 78, 1168, 1752
HpySE526I ACGT 4 cut(s) 153, 171, 709, 1326
Hsp92I GRCGYC 1 cut(s) 1757
Hsp92II CATG 8 cut(s) 348, 509, 611, 913, 966, 1593, 1601, 1909
HspAI GCGC 3 cut(s) 384, 1295, 1757
KasI GGCGCC 1 cut(s) 1756
Kpn2I TCCGGA 1 cut(s) 1071
KpnI GGTACC 1 cut(s) 701
Kzo9I GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 539, 935, 1432, 1507, 1730, 2029, 2181
LweI GCATC 8 cut(s) 73, 632, 748, 753, 775, 1096, 1153, 1328
MaeI CTAG 6 cut(s) 770, 1557, 1568, 1704, 1866, 2135
MaeII ACGT 4 cut(s) 153, 171, 709, 1326
MaeIII GTNAC 7 cut(s) 61, 521, 920, 946, 1644, 1975, 2101
MalI GATC 6 cut(s) 204, 565, 858, 1469, 1782, 1990
MboI GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
MboII GAAGA 9 cut(s) 140, 203, 298, 587, 1184, 1187, 1349, 1418, 1954
MfeI CAATTG 2 cut(s) 867, 1019
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 5 cut(s) 135, 544, 1440, 1460, 2186
MlsI TGGCCA 1 cut(s) 433
MluNI TGGCCA 1 cut(s) 433
Mly113I GGCGCC 1 cut(s) 1757
MlyI GAGTC 5 cut(s) 446, 590, 593, 1041, 2028
MmeI TCCRAC 3 cut(s) 805, 1309, 1966
MnlI CCTC 9 cut(s) 41, 480, 683, 686, 821, 1036, 1079, 1446, 1450
Mox20I TGGCCA 1 cut(s) 433
MroI TCCGGA 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 433
MseI TTAA 6 cut(s) 234, 852, 1091, 1185, 1887, 2209
Msp20I TGGCCA 1 cut(s) 433
MspA1I CMGCKG 3 cut(s) 635, 917, 1256
MspI CCGG 3 cut(s) 932, 1072, 1649
MspR9I CCNGG 5 cut(s) 933, 1147, 1506, 1767, 1855
MunI CAATTG 2 cut(s) 867, 1019
Mva1269I GAATGC 1 cut(s) 776
MvaI CCWGG 4 cut(s) 1147, 1506, 1767, 1855
MwoI GCNNNNNNNGC 8 cut(s) 83, 259, 629, 713, 1292, 1613, 1721, 1730
NarI GGCGCC 1 cut(s) 1757
NciI CCSGG 1 cut(s) 933
NcoI CCATGG 3 cut(s) 505, 909, 962
NdeI CATATG 2 cut(s) 1432, 1618
NdeII GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
NlaIII CATG 8 cut(s) 348, 509, 611, 913, 966, 1593, 1601, 1909
NmuCI GTSAC 4 cut(s) 946, 1644, 1975, 2101
NspI RCATGY 1 cut(s) 1593
PaqCI CACCTGC 1 cut(s) 1115
PceI AGGCCT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 776
PfeI GAWTC 1 cut(s) 2050
PflMI CCANNNNNTGG 3 cut(s) 483, 1541, 1766
PfoI TCCNGGA 2 cut(s) 931, 1504
PleI GAGTC 5 cut(s) 445, 590, 592, 1041, 2028
PluTI GGCGCC 1 cut(s) 1760
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
PpsI GAGTC 5 cut(s) 445, 590, 592, 1041, 2028
Ppu21I YACGTR 2 cut(s) 154, 710
PshBI ATTAAT 1 cut(s) 1887
Psp6I CCWGG 4 cut(s) 1145, 1504, 1765, 1853
PspCI CACGTG 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 1644
PspFI CCCAGC 3 cut(s) 635, 676, 1226
PspGI CCWGG 4 cut(s) 1145, 1504, 1765, 1853
PspOMI GGGCCC 2 cut(s) 131, 1456
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 1 cut(s) 202
PvuII CAGCTG 2 cut(s) 635, 1256
RsaI GTAC 3 cut(s) 699, 722, 1778
RsaNI GTAC 3 cut(s) 698, 721, 1777
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 234, 852, 1091, 1185, 1887, 2209
Sau3AI GATC 6 cut(s) 202, 563, 856, 1467, 1780, 1988
SchI GAGTC 5 cut(s) 446, 590, 593, 1041, 2028
ScrFI CCNGG 5 cut(s) 933, 1147, 1506, 1767, 1855
SduI GDGCHC 5 cut(s) 135, 544, 1440, 1460, 2186
SfaNI GCATC 8 cut(s) 73, 632, 748, 753, 775, 1096, 1153, 1328
SfcI CTRYAG 1 cut(s) 1602
SfoI GGCGCC 1 cut(s) 1758
SinI GGWCC 2 cut(s) 467, 929
SmlI CTYRAG 2 cut(s) 598, 1484
SmoI CTYRAG 2 cut(s) 598, 1484
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 6 cut(s) 603, 621, 773, 915, 997, 1499
SspDI GGCGCC 1 cut(s) 1756
SspI AATATT 1 cut(s) 43
SspMI CTAG 6 cut(s) 770, 1557, 1568, 1704, 1866, 2135
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 5 cut(s) 931, 1145, 1504, 1765, 1853
StyI CCWWGG 4 cut(s) 505, 909, 962, 1011
TaaI ACNGT 9 cut(s) 373, 422, 546, 554, 646, 800, 946, 1829, 1975
TaiI ACGT 4 cut(s) 156, 174, 712, 1329
TaqI TCGA 5 cut(s) 10, 562, 780, 1218, 1451
TatI WGTACW 1 cut(s) 720
TauI GCSGC 3 cut(s) 776, 917, 1502
TfiI GAWTC 1 cut(s) 2050
Tru1I TTAA 6 cut(s) 234, 852, 1091, 1185, 1887, 2209
Tru9I TTAA 6 cut(s) 234, 852, 1091, 1185, 1887, 2209
TscAI CASTG 3 cut(s) 427, 651, 1834
TseFI GTSAC 4 cut(s) 946, 1644, 1975, 2101
Tsp45I GTSAC 4 cut(s) 946, 1644, 1975, 2101
TspDTI ATGAA 4 cut(s) 994, 1305, 1803, 1922
TspGWI ACGGA 2 cut(s) 811, 1743
TspRI CASTG 3 cut(s) 427, 651, 1834
Van91I CCANNNNNTGG 3 cut(s) 483, 1541, 1766
VpaK11BI GGWCC 2 cut(s) 467, 929
VspI ATTAAT 1 cut(s) 1887
XapI RAATTY 4 cut(s) 426, 1079, 1718, 1946
XbaI TCTAGA 1 cut(s) 1567
XceI RCATGY 1 cut(s) 1593
XcmI CCANNNNNNNNNTGG 2 cut(s) 480, 1018
XspI CTAG 6 cut(s) 770, 1557, 1568, 1704, 1866, 2135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.