RLG00000027190

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Reverse (-)
9423001 .. 9426292
3292 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027190

Sequence Viewer

Length: 2235 bp
ATGCCTCTTCGATGGCTACTCCTTCTCAGCCTCAATGCCAATATTCTACTTCTTATTGATGCTACTCAATTAGTTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGATGTGGTTGACAACAACATTAATAATGATATTTCACATGAAGCTCTGCTTCTTCACAGCTACGGGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGCTGGAATGGATGGTGTAGTGTCAATCTTCCCAAGCAAAAAGAAGAGCTACAAAACAACAAAGTCATGGGACTTCCTTGGGTTTCCACAAACGGTGGAGATAGGCCCCCTTGAAAGGGAAATCATCATTGGTGTGATCGATAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCTAAAAAATGGAAAGGCATGTGTCAAGGCAATGAGAACTTTACTTGTAACAACAAAATCATCGGAGCACGGTATTACCATCATCCTCGACCCTTCAAAGAAGGAGAAATTGAGTCTCCAAGAGACTCAGATGGTCATGGAACCCACTGCGCATCAACAGTAGCTGGGAATTTAGTGAGAAATGCGAGCCAAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGGAGTGCCATCCGCACATATTGCGGTGTACAAAGTATGCTGGGAGGATAACTGTGATGAAGCTGACGTTTTAAGGGCATTTGATGATGCCATTGCTGATGGTGTCGACATTATCACTGCTTCCATTGGAGGTGAAGTTCCAAATGATTTTTTGCATGACTCACATATAATTGGGTCATTTCACGCCATAAAAAATGGGATATTGTCTTCGATGGCCGCTGGTAACGAAGGTCCAAAAAAAAAAACTGTGACAAACTTTGTCCCATGGGATCTGTCTGTGGCTGCTACCACCATAAACCGCCAGTTCATCACCAAGCTTCAATTGGGTAACGGTAAAATCTATGAGGGAATTTCACCTAACGTATATGATCTCAAGGGTAAATTCCATCTTTTAATTTATGGTGGAGACGCACCCAATAAAACAGAAGGTGGTAACAAGACTATGTCTAGGTATTGTTCCCCAAATAGCTTAGCAAAAGAATTGGTCAAAGGTAAAATTGTGCTTTGTGATAAAGGCCCTACTAAGGATGCGATTGAGAATGGGGTTGGGGCCATACAGGGTGGTGCAATCGGAGTTATTCTGACGGGCGAAAAACTTGCCGAGGAGTCGCCTGACGCTTATCCCATCCCTTCAACTTATGTCGATTTTGAAGAAAATAGCAACATTTACAAATACATAAATCAAACGGGGAACCCCACTGCAACAATTTGGAAAAGTCAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTATTTCTCATCAAGAGGTCCAAATCCTATGAATGCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCAGTTCCCCCAACTGATGGTGACAGTAGAGCTGGATATGACTTTAAAACTGGGACATCAATGGCATGCCCCCATGCTACTGCCGTTGCTGCATACGTCAAATCATTTCACCCTGAATGGTCACCAGCTGCTATCAAATCAGCTCTCATCACTACCGCTAAACCTATGAGTGCCAAAACTAGCCCGCAAGCTGAATTTGCATATGGAGCTGGCCTAATCAACCCTTCTAGGGCTCCATATCCTGGTTTGATATACGATCTTGATGTACAAGACTATGTAGATTTTTTGTGTTCACAAGGATATAGTGGTAATCTATTGGAACAAGTAACCGGGGACAAGACTAGCTCATGCTCATCAAAATCTAATAATGAAAAAGCCATTGATCTGAACTATCCTTCTTTTACTCTTTGCATTAAGGATCCGAAATTCGTCAATGGGGTCTTCCATAGAACTGTCACTAATGTTGGATCATCGAACTCCACATACATAGCTAAACTGTTGGCTCCATCGGGACTCAAAATCAATGTGGATCCAAGTATGTTGTCGTTCACATCTCTAGGACAAAAGAAATCTTTTGTTGTCACAGTTAAAGGGCCGATTGAGAAATCAAATATAGCCTCTGCGTCTTTGGTGTGGGATGATGGTACTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

745

Amino Acids

80.29

Weight (kDa)

6.27

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 111 1.5e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 586 9.1e-43 Subtilase family
fn3_6 PF17766 643 - 740 2.4e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 2 cut(s) 1562, 1787
AccI GTMKAC 1 cut(s) 813
AciI CCGC 7 cut(s) 720, 731, 924, 1006, 1520, 1701, 1730
AclWI GGATC 6 cut(s) 984, 1958, 1971, 2020, 2069, 2082
AcoI YGGCCR 2 cut(s) 443, 921
AcsI RAATTY 6 cut(s) 438, 655, 1056, 1088, 1739, 1970
AfaI GTAC 3 cut(s) 737, 1812, 2191
AfiI CCNNNNNNNGG 7 cut(s) 128, 405, 406, 1231, 1562, 1774, 1787
AgsI TTSAA 5 cut(s) 404, 583, 1028, 1341, 1358
AjnI CCWGG 2 cut(s) 1525, 1786
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 3 cut(s) 603, 606, 1107
AlwI GGATC 6 cut(s) 984, 1958, 1971, 2020, 2069, 2082
AlwNI CAGNNNCTG 1 cut(s) 650
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 7 cut(s) 394, 443, 921, 1222, 1257, 1756, 2138
ApeKI GCWGC 5 cut(s) 257, 989, 1544, 1634, 1673
ApoI RAATTY 6 cut(s) 438, 655, 1056, 1088, 1739, 1970
AseI ATTAAT 2 cut(s) 192, 1434
AspLEI GCGC 1 cut(s) 638
AspS9I GGNCC 7 cut(s) 395, 479, 938, 1223, 1257, 1478, 2138
AsuC2I CCSGG 1 cut(s) 1876
AsuHPI GGTGA 6 cut(s) 851, 1009, 1053, 1577, 1646, 1659
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 3 cut(s) 479, 938, 1478
BaeGI GKGCMC 1 cut(s) 1461
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 1963, 2074
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 1780, 2210
BbsI GAAGAC 2 cut(s) 906, 1978
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 5 cut(s) 244, 976, 1556, 1621, 1660
BceAI ACGGC 1 cut(s) 1613
BcgI CGANNNNNNTGC 2 cut(s) 1286, 1320
BciT130I CCWGG 2 cut(s) 1527, 1788
BcnI CCSGG 1 cut(s) 1876
BcoDI GTCTC 3 cut(s) 603, 606, 1107
BfaI CTAG 7 cut(s) 290, 1155, 1541, 1725, 1773, 1887, 2102
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 7 cut(s) 258, 924, 990, 1521, 1545, 1635, 1674
BlpI GCTNAGC 1 cut(s) 1177
BlsI GCNGC 7 cut(s) 259, 925, 991, 1522, 1546, 1636, 1675
Bme1390I CCNGG 3 cut(s) 1527, 1788, 1876
Bme18I GGWCC 3 cut(s) 479, 938, 1478
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 7 cut(s) 395, 479, 938, 1223, 1257, 1478, 2138
BmrFI CCNGG 3 cut(s) 1527, 1788, 1876
BmrI ACTGGG 1 cut(s) 1605
BmsI GCATC 5 cut(s) 49, 73, 647, 784, 1225
BmuI ACTGGG 1 cut(s) 1605
BpiI GAAGAC 2 cut(s) 906, 1978
BpmI CTGGAG 1 cut(s) 1509
Bpu1102I GCTNAGC 1 cut(s) 1177
BpuEI CTTGAG 2 cut(s) 1064, 1490
BpuMI CCSGG 1 cut(s) 1876
Bsa29I ATCGAT 1 cut(s) 429
BsaJI CCNNGG 5 cut(s) 247, 367, 971, 1308, 1875
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 405, 406, 1231, 1562, 1774, 1787
Bse1I ACTGG 3 cut(s) 137, 1009, 1600
Bse3DI GCAATG 3 cut(s) 267, 523, 798
BseBI CCWGG 2 cut(s) 1527, 1788
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 5 cut(s) 247, 367, 971, 1308, 1875
BseGI GGATG 7 cut(s) 88, 307, 568, 716, 1240, 1332, 2188
BseLI CCNNNNNNNGG 7 cut(s) 128, 405, 406, 1231, 1562, 1774, 1787
BseMI GCAATG 3 cut(s) 267, 523, 798
BseMII CTCAG 2 cut(s) 40, 627
BseNI ACTGG 3 cut(s) 137, 1009, 1600
BseRI GAGGAG 3 cut(s) 720, 723, 1325
BseSI GKGCMC 1 cut(s) 1461
BseXI GCAGC 5 cut(s) 244, 976, 1556, 1621, 1660
BseYI CCCAGC 2 cut(s) 650, 747
BshFI GGCC 7 cut(s) 396, 445, 923, 1224, 1259, 1758, 2140
BshNI GGYRCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 1 cut(s) 1875
BslFI GGGAC 5 cut(s) 374, 953, 1612, 1892, 2070
BslI CCNNNNNNNGG 7 cut(s) 128, 405, 406, 1231, 1562, 1774, 1787
BsmAI GTCTC 3 cut(s) 603, 606, 1107
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 5 cut(s) 374, 953, 1612, 1892, 2070
BsmI GAATGC 1 cut(s) 1498
BsnI GGCC 7 cut(s) 396, 445, 923, 1224, 1259, 1758, 2140
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 4 cut(s) 556, 1461, 1780, 2210
Bsp1407I TGTACA 2 cut(s) 735, 1810
Bsp143I GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
Bsp1720I GCTNAGC 1 cut(s) 1177
Bsp19I CCATGG 2 cut(s) 247, 971
BspACI CCGC 7 cut(s) 720, 731, 924, 1006, 1520, 1701, 1730
BspANI GGCC 7 cut(s) 396, 445, 923, 1224, 1259, 1758, 2140
BspCNI CTCAG 2 cut(s) 39, 626
BspDI ATCGAT 1 cut(s) 429
BspPI GGATC 6 cut(s) 984, 1958, 1971, 2020, 2069, 2082
BspQI GCTCTTC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 3 cut(s) 267, 523, 798
BsrGI TGTACA 2 cut(s) 735, 1810
BsrI ACTGG 3 cut(s) 137, 1009, 1600
BssECI CCNNGG 5 cut(s) 247, 367, 971, 1308, 1875
BssMI GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
BssT1I CCWWGG 3 cut(s) 247, 367, 971
Bst2UI CCWGG 2 cut(s) 1527, 1788
Bst6I CTCTTC 2 cut(s) 12, 329
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 2 cut(s) 735, 1810
BstC8I GCNNGC 5 cut(s) 673, 1612, 1730, 1734, 1756
BstDEI CTNAG 4 cut(s) 26, 613, 1177, 1230
BstDSI CCRYGG 2 cut(s) 247, 971
BstEII GGTNACC 1 cut(s) 1665
BstF5I GGATG 7 cut(s) 88, 307, 568, 716, 1240, 1332, 2188
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 9 cut(s) 151, 429, 979, 1078, 1804, 1930, 1966, 2015, 2077
BstMAI GTCTC 3 cut(s) 603, 606, 1107
BstMBI GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
BstMWI GCNNNNNNNGC 6 cut(s) 83, 92, 728, 1634, 1742, 1751
BstNI CCWGG 2 cut(s) 1527, 1788
BstNSI RCATGY 2 cut(s) 508, 1614
BstPI GGTNACC 1 cut(s) 1665
BstSCI CCNGG 3 cut(s) 1525, 1786, 1874
BstSLI GKGCMC 1 cut(s) 1461
BstV1I GCAGC 5 cut(s) 244, 976, 1556, 1621, 1660
BstV2I GAAGAC 2 cut(s) 906, 1978
BstX2I RGATCY 3 cut(s) 976, 1963, 2074
BstYI RGATCY 3 cut(s) 976, 1963, 2074
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 7 cut(s) 396, 445, 923, 1224, 1259, 1758, 2140
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 2 cut(s) 247, 971
BtsCI GGATG 7 cut(s) 88, 307, 568, 716, 1240, 1332, 2188
BtsI GCAGTG 3 cut(s) 631, 822, 1404
BtsIMutI CAGTG 3 cut(s) 631, 822, 1404
Cac8I GCNNGC 5 cut(s) 673, 1612, 1730, 1734, 1756
CaiI CAGNNNCTG 1 cut(s) 650
CfoI GCGC 1 cut(s) 638
Cfr13I GGNCC 7 cut(s) 395, 479, 938, 1223, 1257, 1478, 2138
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 3 cut(s) 1124, 1331, 2157
Csp6I GTAC 3 cut(s) 736, 1811, 2190
CviQI GTAC 3 cut(s) 736, 1811, 2190
DdeI CTNAG 4 cut(s) 26, 613, 1177, 1230
DpnI GATC 9 cut(s) 150, 428, 978, 1077, 1803, 1929, 1965, 2014, 2076
DpnII GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
DraI TTTAAA 1 cut(s) 1591
EaeI YGGCCR 2 cut(s) 443, 921
Eam1104I CTCTTC 2 cut(s) 12, 329
EarI CTCTTC 2 cut(s) 12, 329
Eco130I CCWWGG 3 cut(s) 247, 367, 971
Eco24I GRGCYC 2 cut(s) 1780, 2210
Eco47I GGWCC 3 cut(s) 479, 938, 1478
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1665
EcoO109I RGGNCCY 2 cut(s) 395, 1223
EcoO65I GGTNACC 1 cut(s) 1665
EcoRII CCWGG 2 cut(s) 1525, 1786
EcoT14I CCWWGG 3 cut(s) 247, 367, 971
EcoT38I GRGCYC 2 cut(s) 1780, 2210
ErhI CCWWGG 3 cut(s) 247, 367, 971
Esp3I CGTCTC 1 cut(s) 1107
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 5 cut(s) 374, 953, 1612, 1892, 2070
FauI CCCGC 1 cut(s) 1737
FauNDI CATATG 2 cut(s) 1453, 1747
FblI GTMKAC 1 cut(s) 813
Fnu4HI GCNGC 7 cut(s) 258, 924, 990, 1521, 1545, 1635, 1674
FokI GGATG 7 cut(s) 95, 314, 555, 703, 1247, 1319, 2195
FriOI GRGCYC 2 cut(s) 1780, 2210
Fsp4HI GCNGC 7 cut(s) 258, 924, 990, 1521, 1545, 1635, 1674
FspBI CTAG 7 cut(s) 290, 1155, 1541, 1725, 1773, 1887, 2102
FspI TGCGCA 1 cut(s) 637
GlaI GCGC 1 cut(s) 637
GluI GCNGC 7 cut(s) 258, 924, 990, 1521, 1545, 1635, 1674
GsaI CCCAGC 2 cut(s) 654, 751
GsuI CTGGAG 1 cut(s) 1509
HaeIII GGCC 7 cut(s) 396, 445, 923, 1224, 1259, 1758, 2140
HapII CCGG 1 cut(s) 1875
HgaI GACGC 3 cut(s) 1124, 1331, 2157
HhaI GCGC 1 cut(s) 638
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HincII GTYRAC 2 cut(s) 181, 814
HindII GTYRAC 2 cut(s) 181, 814
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 6 cut(s) 449, 599, 611, 866, 1313, 2058
HpaII CCGG 1 cut(s) 1875
HphI GGTGA 6 cut(s) 851, 1009, 1053, 1577, 1646, 1659
Hpy166II GTNNAC 5 cut(s) 181, 736, 814, 1838, 2094
Hpy188I TCNGA 7 cut(s) 454, 551, 616, 1280, 1290, 1932, 1968
Hpy188III TCNNGA 6 cut(s) 80, 1427, 1473, 1805, 2055, 2203
Hpy8I GTNNAC 5 cut(s) 181, 736, 814, 1838, 2094
HpyAV CCTTC 8 cut(s) 32, 581, 589, 929, 1127, 1347, 1779, 1950
HpyCH4IV ACGT 3 cut(s) 774, 1068, 1641
HpyCH4V TGCA 7 cut(s) 260, 862, 1274, 1409, 1637, 1745, 1956
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 92, 728, 1634, 1742, 1751
HpyF3I CTNAG 4 cut(s) 26, 613, 1177, 1230
HpySE526I ACGT 3 cut(s) 774, 1068, 1641
HspAI GCGC 1 cut(s) 636
Kzo9I GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 7 cut(s) 551, 1453, 1528, 1751, 1783, 2053, 2205
Lsp1109I GCAGC 5 cut(s) 244, 976, 1556, 1621, 1660
LweI GCATC 5 cut(s) 49, 73, 647, 784, 1225
MaeI CTAG 7 cut(s) 290, 1155, 1541, 1725, 1773, 1887, 2102
MaeII ACGT 3 cut(s) 774, 1068, 1641
MalI GATC 9 cut(s) 150, 428, 978, 1077, 1803, 1929, 1965, 2014, 2076
MboI GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
MboII GAAGA 7 cut(s) 143, 215, 310, 346, 906, 1370, 1978
MfeI CAATTG 1 cut(s) 1028
MflI RGATCY 3 cut(s) 976, 1963, 2074
MhlI GDGCHC 4 cut(s) 556, 1461, 1780, 2210
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 6 cut(s) 458, 605, 608, 860, 1322, 2052
MmeI TCCRAC 2 cut(s) 432, 1990
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 8 cut(s) 192, 779, 1100, 1434, 1590, 1959, 2133, 2233
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 2 cut(s) 926, 1673
MspI CCGG 1 cut(s) 1875
MspR9I CCNGG 3 cut(s) 1527, 1788, 1876
MunI CAATTG 1 cut(s) 1028
Mva1269I GAATGC 1 cut(s) 1498
MvaI CCWGG 2 cut(s) 1527, 1788
MwoI GCNNNNNNNGC 6 cut(s) 83, 92, 728, 1634, 1742, 1751
NciI CCSGG 1 cut(s) 1876
NcoI CCATGG 2 cut(s) 247, 971
NdeI CATATG 2 cut(s) 1453, 1747
NdeII GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
NmeAIII GCCGAG 1 cut(s) 1333
NmuCI GTSAC 5 cut(s) 955, 1565, 1665, 1999, 2125
NsbI TGCGCA 1 cut(s) 637
NspI RCATGY 2 cut(s) 508, 1614
PaeI GCATGC 1 cut(s) 1614
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 1 cut(s) 1498
PflFI GACNNNGTC 1 cut(s) 1150
PflMI CCANNNNNTGG 2 cut(s) 1562, 1787
PfoI TCCNGGA 1 cut(s) 1525
PkrI GCNGC 7 cut(s) 259, 925, 991, 1522, 1546, 1636, 1675
PleI GAGTC 6 cut(s) 457, 605, 607, 860, 1321, 2052
PpsI GAGTC 6 cut(s) 457, 605, 607, 860, 1321, 2052
PshBI ATTAAT 2 cut(s) 192, 1434
Psp6I CCWGG 2 cut(s) 1525, 1786
PspEI GGTNACC 1 cut(s) 1665
PspFI CCCAGC 2 cut(s) 650, 747
PspGI CCWGG 2 cut(s) 1525, 1786
PspPI GGNCC 7 cut(s) 395, 479, 938, 1223, 1257, 1478, 2138
PsrI GAACNNNNNNTAC 2 cut(s) 2012, 2044
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 3 cut(s) 976, 1963, 2074
PsyI GACNNNGTC 1 cut(s) 1150
PvuII CAGCTG 1 cut(s) 1673
RsaI GTAC 3 cut(s) 737, 1812, 2191
RsaNI GTAC 3 cut(s) 736, 1811, 2190
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 8 cut(s) 192, 779, 1100, 1434, 1590, 1959, 2133, 2233
SatI GCNGC 7 cut(s) 258, 924, 990, 1521, 1545, 1635, 1674
Sau3AI GATC 9 cut(s) 148, 426, 976, 1075, 1801, 1927, 1963, 2012, 2074
Sau96I GGNCC 7 cut(s) 395, 479, 938, 1223, 1257, 1478, 2138
SchI GAGTC 6 cut(s) 458, 605, 608, 860, 1322, 2052
ScrFI CCNGG 3 cut(s) 1527, 1788, 1876
SduI GDGCHC 4 cut(s) 556, 1461, 1780, 2210
SfaNI GCATC 5 cut(s) 49, 73, 647, 784, 1225
SinI GGWCC 3 cut(s) 479, 938, 1478
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 1079, 1505
SmoI CTYRAG 2 cut(s) 1079, 1505
SphI GCATGC 1 cut(s) 1614
SsiI CCGC 7 cut(s) 720, 731, 924, 1006, 1520, 1701, 1730
SspI AATATT 2 cut(s) 43, 163
SspMI CTAG 7 cut(s) 290, 1155, 1541, 1725, 1773, 1887, 2102
StyD4I CCNGG 3 cut(s) 1525, 1786, 1874
StyI CCWWGG 3 cut(s) 247, 367, 971
TaiI ACGT 3 cut(s) 777, 1071, 1644
TaqI TCGA 7 cut(s) 10, 429, 574, 813, 917, 1350, 2018
TatI WGTACW 2 cut(s) 735, 1810
TauI GCSGC 2 cut(s) 926, 1523
Tru1I TTAA 8 cut(s) 192, 779, 1100, 1434, 1590, 1959, 2133, 2233
Tru9I TTAA 8 cut(s) 192, 779, 1100, 1434, 1590, 1959, 2133, 2233
TscAI CASTG 3 cut(s) 638, 829, 1411
TseFI GTSAC 5 cut(s) 955, 1565, 1665, 1999, 2125
TseI GCWGC 5 cut(s) 257, 989, 1544, 1634, 1673
Tsp45I GTSAC 5 cut(s) 955, 1565, 1665, 1999, 2125
TspDTI ATGAA 6 cut(s) 143, 225, 780, 1003, 1505, 1929
TspRI CASTG 3 cut(s) 638, 829, 1411
Tth111I GACNNNGTC 1 cut(s) 1150
Van91I CCANNNNNTGG 2 cut(s) 1562, 1787
VpaK11BI GGWCC 3 cut(s) 479, 938, 1478
VspI ATTAAT 2 cut(s) 192, 1434
XapI RAATTY 6 cut(s) 438, 655, 1056, 1088, 1739, 1970
XceI RCATGY 2 cut(s) 508, 1614
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1027
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 7 cut(s) 290, 1155, 1541, 1725, 1773, 1887, 2102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.