Rh1BG059100

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000390
Physical Location & Seq
Reverse (-)
8709200 .. 8713695
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG059100.1

Sequence Viewer

Length: 2178 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTCGATGTTACTCACTCAACTGCTCAGGATACCCGAAAGACCTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAAGGAGCGTCAAAACAACAAAGTCATGGAACTTCCTGGGGTTTCCAGAAACAGTTAAGAGAAGCGCCCTTGAAAGGGATATCATCGTCGGTGTGATGGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTAATTGTAACAATAAAATCATTGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAGAAGATGTTGAGTCTCCAAGAGACTCAAGCGGTCATGGAACTCATACCGCATCAACGGCAGCTGGGAACATAGTGAGCAAGGCAAGCCTAAAAGGTTTAGGGTTGGGAACAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCCGTGTACAAAGTATGCTGGGGCAGCAGCATCAGTTGTGAAGATGTTGACATTCTAGCGGCATTCGATGATGCCATTGCTGATGGTGTCGACATTCTCTCTCTTTCTGTCGGGGGCCATATTCCAGTTGATTATTTGACTAATTCACTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCACCCAATAAAAAGGCAGGTGTTACAGGGACCTCCTCCAGGTTTTGTCTCATAGACACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTATGAAGAAGGCTGGGGATGGGGCCATATTGGGTGGTGCAGCCGGAATTGTTCTGACGGGACGAAAGGTCGCCGATAAAGTGCTTGGCCCTCTTCCCGTGCCTGCATCACACGTCGGGTTGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGAGGTGGTGGCAGTAGAGCTGGATACTATATAGCGACCGGGACATCAATGGCGTGCCCCCATGCTACGGCCGTAGCTGCATACGTCAAATCATTTCATCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGCTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAGATCTAATAATGGAACAGCCAATAACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCATCGAATTCCACATACAGAGGTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTTTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGTGTCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

725

Amino Acids

77.28

Weight (kDa)

8.92

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 112 6e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 568 2.5e-42 Subtilase family
fn3_6 PF17766 624 - 721 4.8e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1124
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 2 cut(s) 133, 706
AccB7I CCANNNNNTGG 2 cut(s) 1508, 1733
AccI GTMKAC 1 cut(s) 813
AciI CCGC 7 cut(s) 612, 630, 782, 924, 1006, 1466, 1647
AclWI GGATC 4 cut(s) 567, 1901, 1914, 1963
AcoI YGGCCR 3 cut(s) 440, 921, 1572
AcsI RAATTY 6 cut(s) 435, 1088, 1685, 1913, 1963, 2098
AfaI GTAC 3 cut(s) 135, 731, 1745
AflIII ACRYGT 1 cut(s) 1333
AgsI TTSAA 4 cut(s) 244, 401, 1028, 1345
AhdI GACNNNNNGTC 1 cut(s) 1289
AhlI ACTAGT 1 cut(s) 1832
AjiI CACGTC 1 cut(s) 1336
AjnI CCWGG 4 cut(s) 363, 1154, 1471, 1732
AluBI AGCT 7 cut(s) 212, 228, 456, 644, 1523, 1580, 1700
AluI AGCT 7 cut(s) 212, 228, 456, 644, 1523, 1580, 1700
Alw21I GWGCWC 2 cut(s) 553, 1784
Alw26I GTCTC 5 cut(s) 597, 600, 1107, 1169, 2110
AlwI GGATC 4 cut(s) 567, 1901, 1914, 1963
AoxI GGCC 9 cut(s) 440, 838, 921, 1219, 1245, 1309, 1572, 1702, 2081
ApoI RAATTY 6 cut(s) 435, 1088, 1685, 1913, 1963, 2098
AseI ATTAAT 1 cut(s) 1380
Asp718I GGTACC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 9 cut(s) 476, 838, 938, 1146, 1220, 1245, 1310, 1424, 2081
AsuC2I CCSGG 3 cut(s) 942, 1082, 1543
AsuHPI GGTGA 2 cut(s) 1009, 1605
AsuII TTCGAA 1 cut(s) 579
AvaII GGWCC 4 cut(s) 476, 938, 1146, 1424
BaeGI GKGCMC 2 cut(s) 1407, 1562
BaeI ACNNNNGTAYC 2 cut(s) 1783, 1816
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1906
BanI GGYRCC 2 cut(s) 133, 706
BanII GRGCYC 2 cut(s) 1726, 2153
BbsI GAAGAC 2 cut(s) 307, 1921
Bbv12I GWGCWC 2 cut(s) 553, 1784
BceAI ACGGC 4 cut(s) 654, 710, 1559, 1587
BciT130I CCWGG 4 cut(s) 365, 1156, 1473, 1734
BciVI GTATCC 3 cut(s) 76, 1520, 1784
BclI TGATCA 1 cut(s) 1191
BcnI CCSGG 3 cut(s) 942, 1082, 1543
BcoDI GTCTC 5 cut(s) 597, 600, 1107, 1169, 2110
BcuI ACTAGT 1 cut(s) 1832
BfaI CTAG 4 cut(s) 779, 1671, 1719, 1833
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 1124
BfuI GTATCC 3 cut(s) 76, 1520, 1784
BglII AGATCT 1 cut(s) 1847
Bme1390I CCNGG 7 cut(s) 365, 942, 1082, 1156, 1473, 1543, 1734
Bme18I GGWCC 4 cut(s) 476, 938, 1146, 1424
BmeRI GACNNNNNGTC 1 cut(s) 1289
BmgBI CACGTC 1 cut(s) 1336
BmgT120I GGNCC 9 cut(s) 476, 838, 938, 1146, 1220, 1245, 1310, 1424, 2081
BmrFI CCNGG 7 cut(s) 365, 942, 1082, 1156, 1473, 1543, 1734
BmsI GCATC 5 cut(s) 641, 762, 784, 1337, 1669
BpiI GAAGAC 2 cut(s) 307, 1921
BpmI CTGGAG 2 cut(s) 1138, 1455
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 2 cut(s) 592, 1436
BpuMI CCSGG 3 cut(s) 942, 1082, 1543
BsaAI YACGTR 1 cut(s) 719
BsaJI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1081, 1821
BsaWI WCCGGW 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 1615
Bse1I ACTGG 1 cut(s) 848
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 4 cut(s) 365, 1156, 1473, 1734
BseDI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1081, 1821
BseGI GGATG 4 cut(s) 304, 1246, 1600, 2131
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 2 cut(s) 40, 92
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 2 cut(s) 714, 1141
BseSI GKGCMC 2 cut(s) 1407, 1562
BseX3I CGGCCG 1 cut(s) 1572
BseYI CCCAGC 3 cut(s) 644, 741, 1235
BsgI GTGCAG 1 cut(s) 1281
Bsh1285I CGRYCG 2 cut(s) 1542, 1575
BshFI GGCC 9 cut(s) 442, 840, 923, 1221, 1247, 1311, 1574, 1704, 2083
BshNI GGYRCC 2 cut(s) 133, 706
BsiEI CGRYCG 2 cut(s) 1542, 1575
BsiHKAI GWGCWC 2 cut(s) 553, 1784
BsiSI CCGG 6 cut(s) 291, 941, 1081, 1266, 1542, 1616
BslFI GGGAC 5 cut(s) 1068, 1159, 1296, 1558, 2013
BsmAI GTCTC 5 cut(s) 597, 600, 1107, 1169, 2110
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 5 cut(s) 1068, 1159, 1296, 1558, 2013
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 9 cut(s) 442, 840, 923, 1221, 1247, 1311, 1574, 1704, 2083
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 6 cut(s) 553, 1407, 1562, 1726, 1784, 2153
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
Bsp19I CCATGG 2 cut(s) 918, 971
BspACI CCGC 7 cut(s) 612, 630, 782, 924, 1006, 1466, 1647
BspANI GGCC 9 cut(s) 442, 840, 923, 1221, 1247, 1311, 1574, 1704, 2083
BspCNI CTCAG 2 cut(s) 39, 91
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 567, 1901, 1914, 1963
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 133, 706
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrFI RCCGGY 1 cut(s) 1615
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 1 cut(s) 848
BssAI RCCGGY 1 cut(s) 1615
BssECI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1081, 1821
BssMI GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
BssT1I CCWWGG 4 cut(s) 918, 971, 1020, 1821
Bst2UI CCWGG 4 cut(s) 365, 1156, 1473, 1734
Bst4CI ACNGT 7 cut(s) 382, 431, 555, 563, 955, 1796, 1942
Bst6I CTCTTC 3 cut(s) 12, 1320, 1368
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 5 cut(s) 667, 1326, 1558, 1702, 1812
BstDEI CTNAG 4 cut(s) 26, 78, 486, 1177
BstDSI CCRYGG 2 cut(s) 918, 971
BstEII GGTNACC 1 cut(s) 1611
BstENI CCTNNNNNAGG 1 cut(s) 1153
BstF5I GGATG 4 cut(s) 304, 1246, 1600, 2131
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 8 cut(s) 151, 575, 1194, 1437, 1750, 1850, 1909, 1958
BstMAI GTCTC 5 cut(s) 597, 600, 1107, 1169, 2110
BstMBI GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
BstMCI CGRYCG 2 cut(s) 1542, 1575
BstNI CCWGG 4 cut(s) 365, 1156, 1473, 1734
BstPI GGTNACC 1 cut(s) 1611
BstSCI CCNGG 7 cut(s) 363, 940, 1080, 1154, 1471, 1541, 1732
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 2 cut(s) 1407, 1562
BstV2I GAAGAC 2 cut(s) 307, 1921
BstX2I RGATCY 2 cut(s) 1847, 1906
BstYI RGATCY 2 cut(s) 1847, 1906
BstZI CGGCCG 1 cut(s) 1572
BsuI GTATCC 3 cut(s) 76, 1520, 1784
BsuRI GGCC 9 cut(s) 442, 840, 923, 1221, 1247, 1311, 1574, 1704, 2083
BtgI CCRYGG 2 cut(s) 918, 971
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 4 cut(s) 304, 1246, 1600, 2131
BtsIMutI CAGTG 2 cut(s) 436, 1801
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 5 cut(s) 667, 1326, 1558, 1702, 1812
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 1615
Cfr13I GGNCC 9 cut(s) 476, 838, 938, 1146, 1220, 1245, 1310, 1424, 2081
CseI GACGC 2 cut(s) 325, 1124
Csp6I GTAC 3 cut(s) 134, 730, 1744
CviAII CATG 7 cut(s) 155, 206, 354, 617, 919, 972, 1565
CviQI GTAC 3 cut(s) 134, 730, 1744
DdeI CTNAG 4 cut(s) 26, 78, 486, 1177
DpnI GATC 8 cut(s) 150, 574, 1193, 1436, 1749, 1849, 1908, 1957
DpnII GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
DriI GACNNNNNGTC 1 cut(s) 1289
EaeI YGGCCR 3 cut(s) 440, 921, 1572
EagI CGGCCG 1 cut(s) 1572
Eam1104I CTCTTC 3 cut(s) 12, 1320, 1368
Eam1105I GACNNNNNGTC 1 cut(s) 1289
EarI CTCTTC 3 cut(s) 12, 1320, 1368
EclXI CGGCCG 1 cut(s) 1572
Eco130I CCWWGG 4 cut(s) 918, 971, 1020, 1821
Eco24I GRGCYC 2 cut(s) 1726, 2153
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 476, 938, 1146, 1424
Eco52I CGGCCG 1 cut(s) 1572
Eco91I GGTNACC 1 cut(s) 1611
EcoNI CCTNNNNNAGG 1 cut(s) 1153
EcoO109I RGGNCCY 1 cut(s) 1146
EcoO65I GGTNACC 1 cut(s) 1611
EcoRI GAATTC 1 cut(s) 1963
EcoRII CCWGG 4 cut(s) 363, 1154, 1471, 1732
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 4 cut(s) 918, 971, 1020, 1821
EcoT38I GRGCYC 2 cut(s) 1726, 2153
ErhI CCWWGG 4 cut(s) 918, 971, 1020, 1821
Esp3I CGTCTC 1 cut(s) 1107
FaeI CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1568
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 5 cut(s) 1068, 1159, 1296, 1558, 2013
FatI CATG 7 cut(s) 154, 205, 353, 616, 918, 971, 1564
FauNDI CATATG 1 cut(s) 1399
FbaI TGATCA 1 cut(s) 1191
FblI GTMKAC 1 cut(s) 813
FokI GGATG 4 cut(s) 311, 1253, 1587, 2138
FriOI GRGCYC 2 cut(s) 1726, 2153
FspBI CTAG 4 cut(s) 779, 1671, 1719, 1833
GlaI GCGC 1 cut(s) 394
GsaI CCCAGC 3 cut(s) 648, 745, 1239
GsuI CTGGAG 2 cut(s) 1138, 1455
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 9 cut(s) 442, 840, 923, 1221, 1247, 1311, 1574, 1704, 2083
HapII CCGG 6 cut(s) 291, 941, 1081, 1266, 1542, 1616
HgaI GACGC 2 cut(s) 325, 1124
HhaI GCGC 1 cut(s) 395
Hin1II CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1568
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 3 cut(s) 181, 772, 814
HindII GTYRAC 3 cut(s) 181, 772, 814
HinfI GANTC 5 cut(s) 446, 593, 605, 2001, 2017
HpaII CCGG 6 cut(s) 291, 941, 1081, 1266, 1542, 1616
HphI GGTGA 2 cut(s) 1009, 1605
Hpy166II GTNNAC 7 cut(s) 181, 730, 772, 814, 1744, 1802, 2037
Hpy188I TCNGA 4 cut(s) 451, 1278, 1434, 1911
Hpy188III TCNNGA 6 cut(s) 80, 374, 570, 1751, 1845, 2146
Hpy8I GTNNAC 7 cut(s) 181, 730, 772, 814, 1744, 1802, 2037
Hpy99I CGWCG 2 cut(s) 419, 1340
HpyAV CCTTC 6 cut(s) 32, 586, 929, 1225, 1725, 1893
HpyCH4III ACNGT 7 cut(s) 382, 431, 555, 563, 955, 1796, 1942
HpyCH4IV ACGT 4 cut(s) 174, 718, 1335, 1587
HpyF3I CTNAG 4 cut(s) 26, 78, 486, 1177
HpySE526I ACGT 4 cut(s) 174, 718, 1335, 1587
Hsp92II CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1568
HspAI GCGC 1 cut(s) 393
KpnI GGTACC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 1191
Kzo9I GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 9 cut(s) 333, 548, 944, 1399, 1474, 1697, 1729, 1996, 2148
LweI GCATC 5 cut(s) 641, 762, 784, 1337, 1669
MaeI CTAG 4 cut(s) 779, 1671, 1719, 1833
MaeII ACGT 4 cut(s) 174, 718, 1335, 1587
MaeIII GTNAC 8 cut(s) 61, 530, 929, 955, 1138, 1611, 1942, 2068
MalI GATC 8 cut(s) 150, 574, 1193, 1436, 1749, 1849, 1908, 1957
MboI GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 2 cut(s) 1847, 1906
MhlI GDGCHC 6 cut(s) 553, 1407, 1562, 1726, 1784, 2153
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 4 cut(s) 455, 599, 602, 1995
MmeI TCCRAC 2 cut(s) 429, 1933
Mox20I TGGCCA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 442
MseI TTAA 5 cut(s) 384, 525, 1100, 1380, 2176
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 644, 926
MspI CCGG 6 cut(s) 291, 941, 1081, 1266, 1542, 1616
MspR9I CCNGG 7 cut(s) 365, 942, 1082, 1156, 1473, 1543, 1734
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 4 cut(s) 365, 1156, 1473, 1734
NciI CCSGG 3 cut(s) 942, 1082, 1543
NcoI CCATGG 2 cut(s) 918, 971
NdeI CATATG 1 cut(s) 1399
NdeII GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
NlaIII CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1568
NmuCI GTSAC 4 cut(s) 955, 1611, 1942, 2068
NspV TTCGAA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 1124
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 1 cut(s) 2017
PflMI CCANNNNNTGG 2 cut(s) 1508, 1733
PfoI TCCNGGA 2 cut(s) 940, 1471
PleI GAGTC 4 cut(s) 454, 599, 601, 1995
PpsI GAGTC 4 cut(s) 454, 599, 601, 1995
Ppu21I YACGTR 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 1146
PshBI ATTAAT 1 cut(s) 1380
Psp5II RGGWCCY 1 cut(s) 1146
Psp6I CCWGG 4 cut(s) 363, 1154, 1471, 1732
PspEI GGTNACC 1 cut(s) 1611
PspFI CCCAGC 3 cut(s) 644, 741, 1235
PspGI CCWGG 4 cut(s) 363, 1154, 1471, 1732
PspPI GGNCC 9 cut(s) 476, 838, 938, 1146, 1220, 1245, 1310, 1424, 2081
PspPPI RGGWCCY 1 cut(s) 1146
PsuI RGATCY 2 cut(s) 1847, 1906
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 3 cut(s) 135, 731, 1745
RsaNI GTAC 3 cut(s) 134, 730, 1744
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 5 cut(s) 384, 525, 1100, 1380, 2176
Sau3AI GATC 8 cut(s) 148, 572, 1191, 1434, 1747, 1847, 1906, 1955
Sau96I GGNCC 9 cut(s) 476, 838, 938, 1146, 1220, 1245, 1310, 1424, 2081
SchI GAGTC 4 cut(s) 455, 599, 602, 1995
ScrFI CCNGG 7 cut(s) 365, 942, 1082, 1156, 1473, 1543, 1734
SduI GDGCHC 6 cut(s) 553, 1407, 1562, 1726, 1784, 2153
SfaNI GCATC 5 cut(s) 641, 762, 784, 1337, 1669
SfcI CTRYAG 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 476, 938, 1146, 1424
SmlI CTYRAG 2 cut(s) 607, 1451
SmoI CTYRAG 2 cut(s) 607, 1451
SpeI ACTAGT 1 cut(s) 1832
SsiI CCGC 7 cut(s) 612, 630, 782, 924, 1006, 1466, 1647
SspMI CTAG 4 cut(s) 779, 1671, 1719, 1833
StyD4I CCNGG 7 cut(s) 363, 940, 1080, 1154, 1471, 1541, 1732
StyI CCWWGG 4 cut(s) 918, 971, 1020, 1821
TaaI ACNGT 7 cut(s) 382, 431, 555, 563, 955, 1796, 1942
TaiI ACGT 4 cut(s) 177, 721, 1338, 1590
TaqI TCGA 7 cut(s) 10, 57, 571, 579, 789, 813, 1961
TatI WGTACW 1 cut(s) 729
TauI GCSGC 3 cut(s) 785, 926, 1469
TfiI GAWTC 1 cut(s) 2017
Tru1I TTAA 5 cut(s) 384, 525, 1100, 1380, 2176
Tru9I TTAA 5 cut(s) 384, 525, 1100, 1380, 2176
TscAI CASTG 2 cut(s) 436, 1801
TseFI GTSAC 4 cut(s) 955, 1611, 1942, 2068
Tsp45I GTSAC 4 cut(s) 955, 1611, 1942, 2068
TspDTI ATGAA 7 cut(s) 143, 222, 1003, 1241, 1589, 1770, 1830
TspGWI ACGGA 1 cut(s) 1710
TspRI CASTG 2 cut(s) 436, 1801
Van91I CCANNNNNTGG 2 cut(s) 1508, 1733
VpaK11BI GGWCC 4 cut(s) 476, 938, 1146, 1424
VspI ATTAAT 1 cut(s) 1380
XagI CCTNNNNNAGG 1 cut(s) 1153
XapI RAATTY 6 cut(s) 435, 1088, 1685, 1913, 1963, 2098
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 4 cut(s) 779, 1671, 1719, 1833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.