Rh1AG363200

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000390
Physical Location & Seq
Reverse (-)
59774916 .. 59776777
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG363200.1

Sequence Viewer

Length: 996 bp
ATGGATGAGGCTGGGGTTGGGGCGATATTGGGTGGCGCAGTCGGAATTGTTCTGACAGGAAAAAAAGTCAACGATGAACTGCTTGGCCCTTATCCGGTGCCTGCAACTTTCGTCGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAATTCAACAAGGAATGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATACGTGCCATCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTAGATACTATATAGAAACTGGGACATCAATGGCATGCCCTCATGCTACCGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCAGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAGTGCCAAAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGGACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGCAAGCCATAACCATGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATTCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTAAGAAATCAAATCTAATCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCAATTGTTGTCTATGTTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

331

Amino Acids

35.25

Weight (kDa)

9.17

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 71 - 174 2e-18 Subtilase family
fn3_6 PF17766 230 - 327 1.1e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 97, 541
AccB7I CCANNNNNTGG 2 cut(s) 326, 551
AciI CCGC 2 cut(s) 284, 390
AclWI GGATC 1 cut(s) 781
AcsI RAATTY 3 cut(s) 151, 503, 731
AcyI GRCGYC 1 cut(s) 542
AfiI CCNNNNNNNGG 5 cut(s) 94, 323, 326, 538, 551
AgsI TTSAA 1 cut(s) 156
AhlI ACTAGT 1 cut(s) 650
AjnI CCWGG 2 cut(s) 289, 550
AluBI AGCT 6 cut(s) 341, 398, 437, 518, 683, 797
AluI AGCT 6 cut(s) 341, 398, 437, 518, 683, 797
AlwI GGATC 1 cut(s) 781
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 4 cut(s) 85, 241, 520, 899
ApaI GGGCCC 1 cut(s) 245
ApeKI GCWGC 3 cut(s) 308, 398, 437
ApoI RAATTY 3 cut(s) 151, 503, 731
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
AseI ATTAAT 1 cut(s) 672
AspLEI GCGC 2 cut(s) 38, 544
AspS9I GGNCC 4 cut(s) 86, 241, 242, 899
AsuHPI GGTGA 2 cut(s) 410, 423
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 245
BaeI ACNNNNGTAYC 2 cut(s) 601, 634
BanI GGYRCC 2 cut(s) 97, 541
BanII GRGCYC 2 cut(s) 245, 971
BbsI GAAGAC 1 cut(s) 739
BbvI GCAGC 3 cut(s) 320, 385, 424
BccI CCATC 4 cut(s) 232, 566, 725, 941
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 2 cut(s) 291, 552
BciVI GTATCC 1 cut(s) 602
BcuI ACTAGT 1 cut(s) 650
BfaI CTAG 4 cut(s) 342, 489, 537, 651
BfoI RGCGCY 1 cut(s) 545
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 4 cut(s) 285, 309, 399, 438
BlsI GCNGC 4 cut(s) 286, 310, 400, 439
Bme1390I CCNGG 2 cut(s) 291, 552
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 4 cut(s) 86, 241, 242, 899
BmiI GGNNCC 8 cut(s) 99, 213, 242, 243, 288, 543, 810, 968
BmrFI CCNGG 2 cut(s) 291, 552
BmrI ACTGGG 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 739
BpmI CTGGAG 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 639
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 793, 823
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 323, 326, 538, 551
Bse1I ACTGG 1 cut(s) 364
BseBI CCWGG 2 cut(s) 291, 552
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 3 cut(s) 10, 224, 949
BseLI CCNNNNNNNGG 5 cut(s) 94, 323, 326, 538, 551
BseNI ACTGG 1 cut(s) 364
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 3 cut(s) 320, 385, 424
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 4 cut(s) 87, 243, 522, 901
BshNI GGYRCC 2 cut(s) 97, 541
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 376, 831
BslI CCNNNNNNNGG 5 cut(s) 94, 323, 326, 538, 551
BsmFI GGGAC 2 cut(s) 376, 831
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 4 cut(s) 87, 243, 522, 901
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 728
Bsp120I GGGCCC 1 cut(s) 241
Bsp1286I GDGCHC 2 cut(s) 245, 971
Bsp143I GATC 3 cut(s) 252, 565, 773
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 2 cut(s) 284, 390
BspANI GGCC 4 cut(s) 87, 243, 522, 901
BspLI GGNNCC 8 cut(s) 99, 213, 242, 243, 288, 543, 810, 968
BspPI GGATC 1 cut(s) 781
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 2 cut(s) 97, 541
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 3 cut(s) 252, 565, 773
BssNI GRCGYC 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 291, 552
Bst4CI ACNGT 2 cut(s) 760, 805
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 4 cut(s) 102, 376, 520, 630
BstDSI CCRYGG 1 cut(s) 639
BstEII GGTNACC 1 cut(s) 429
BstF5I GGATG 3 cut(s) 10, 224, 949
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 38, 544
BstKTI GATC 3 cut(s) 255, 568, 776
BstMBI GATC 3 cut(s) 252, 565, 773
BstMWI GCNNNNNNNGC 3 cut(s) 398, 506, 515
BstNI CCWGG 2 cut(s) 291, 552
BstNSI RCATGY 1 cut(s) 378
BstPI GGTNACC 1 cut(s) 429
BstSCI CCNGG 2 cut(s) 289, 550
BstSLI GKGCMC 1 cut(s) 245
BstV1I GCAGC 3 cut(s) 320, 385, 424
BstV2I GAAGAC 1 cut(s) 739
BstXI CCANNNNNNTGG 1 cut(s) 640
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 4 cut(s) 87, 243, 522, 901
BtgI CCRYGG 1 cut(s) 639
BtsCI GGATG 3 cut(s) 10, 224, 949
Cac8I GCNNGC 4 cut(s) 102, 376, 520, 630
CfoI GCGC 2 cut(s) 38, 544
Cfr13I GGNCC 4 cut(s) 86, 241, 242, 899
CseI GACGC 2 cut(s) 506, 918
CviAII CATG 4 cut(s) 375, 383, 640, 691
DinI GGCGCC 1 cut(s) 543
DpnI GATC 3 cut(s) 254, 567, 775
DpnII GATC 3 cut(s) 252, 565, 773
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 639
Eco24I GRGCYC 2 cut(s) 245, 971
Eco88I CYCGRG 1 cut(s) 863
Eco91I GGTNACC 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 429
EcoRII CCWGG 2 cut(s) 289, 550
EcoT14I CCWWGG 1 cut(s) 639
EcoT38I GRGCYC 2 cut(s) 245, 971
EgeI GGCGCC 1 cut(s) 543
EheI GGCGCC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 4 cut(s) 378, 386, 643, 694
FaqI GGGAC 2 cut(s) 376, 831
FatI CATG 4 cut(s) 374, 382, 639, 690
Fnu4HI GCNGC 4 cut(s) 285, 309, 399, 438
FokI GGATG 3 cut(s) 17, 211, 956
FriOI GRGCYC 2 cut(s) 245, 971
Fsp4HI GCNGC 4 cut(s) 285, 309, 399, 438
FspBI CTAG 4 cut(s) 342, 489, 537, 651
GlaI GCGC 2 cut(s) 37, 543
GluI GCNGC 4 cut(s) 285, 309, 399, 438
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 4 cut(s) 87, 243, 522, 901
HapII CCGG 1 cut(s) 95
HgaI GACGC 2 cut(s) 506, 918
HhaI GCGC 2 cut(s) 38, 544
Hin1I GRCGYC 1 cut(s) 542
Hin1II CATG 4 cut(s) 378, 386, 643, 694
Hin6I GCGC 2 cut(s) 36, 542
HinP1I GCGC 2 cut(s) 36, 542
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 3 cut(s) 725, 819, 835
HpaII CCGG 1 cut(s) 95
HphI GGTGA 2 cut(s) 410, 423
Hpy166II GTNNAC 4 cut(s) 70, 602, 620, 855
Hpy188I TCNGA 3 cut(s) 44, 54, 252
Hpy188III TCNNGA 2 cut(s) 569, 964
Hpy8I GTNNAC 4 cut(s) 70, 602, 620, 855
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 543, 711
HpyCH4III ACNGT 2 cut(s) 760, 805
HpyCH4IV ACGT 2 cut(s) 219, 405
HpyCH4V TGCA 7 cut(s) 104, 167, 173, 401, 509, 628, 710
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 506, 515
HpySE526I ACGT 2 cut(s) 219, 405
Hsp92I GRCGYC 1 cut(s) 542
Hsp92II CATG 4 cut(s) 378, 386, 643, 694
HspAI GCGC 2 cut(s) 36, 542
KasI GGCGCC 1 cut(s) 541
Kzo9I GATC 3 cut(s) 252, 565, 773
LmnI GCTCC 5 cut(s) 217, 292, 515, 814, 966
Lsp1109I GCAGC 3 cut(s) 320, 385, 424
MaeI CTAG 4 cut(s) 342, 489, 537, 651
MaeII ACGT 2 cut(s) 219, 405
MaeIII GTNAC 3 cut(s) 429, 760, 886
MalI GATC 3 cut(s) 254, 567, 775
MboI GATC 3 cut(s) 252, 565, 773
MboII GAAGA 3 cut(s) 134, 203, 739
MfeI CAATTG 1 cut(s) 972
MhlI GDGCHC 2 cut(s) 245, 971
MluCI AATT 5 cut(s) 45, 151, 503, 731, 972
Mly113I GGCGCC 1 cut(s) 542
MlyI GAGTC 1 cut(s) 813
MmeI TCCRAC 3 cut(s) 22, 99, 751
MnlI CCTC 2 cut(s) 231, 390
MseI TTAA 3 cut(s) 672, 906, 994
MslI CAYNNNNRTG 1 cut(s) 638
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 291, 552
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 2 cut(s) 291, 552
MwoI GCNNNNNNNGC 3 cut(s) 398, 506, 515
NarI GGCGCC 1 cut(s) 542
NcoI CCATGG 1 cut(s) 639
NdeII GATC 3 cut(s) 252, 565, 773
NlaIII CATG 4 cut(s) 378, 386, 643, 694
NlaIV GGNNCC 8 cut(s) 99, 213, 242, 243, 288, 543, 810, 968
NmuCI GTSAC 3 cut(s) 429, 760, 886
NspI RCATGY 1 cut(s) 378
NspV TTCGAA 1 cut(s) 728
PaeI GCATGC 1 cut(s) 378
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 725, 835
PflMI CCANNNNNTGG 2 cut(s) 326, 551
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 4 cut(s) 286, 310, 400, 439
PleI GAGTC 1 cut(s) 813
PluTI GGCGCC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 813
Ppu21I YACGTR 1 cut(s) 220
PshBI ATTAAT 1 cut(s) 672
Psp6I CCWGG 2 cut(s) 289, 550
PspEI GGTNACC 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 289, 550
PspN4I GGNNCC 8 cut(s) 99, 213, 242, 243, 288, 543, 810, 968
PspOMI GGGCCC 1 cut(s) 241
PspPI GGNCC 4 cut(s) 86, 241, 242, 899
PvuII CAGCTG 1 cut(s) 437
RseI CAYNNNNRTG 1 cut(s) 638
SaqAI TTAA 3 cut(s) 672, 906, 994
SatI GCNGC 4 cut(s) 285, 309, 399, 438
Sau3AI GATC 3 cut(s) 252, 565, 773
Sau96I GGNCC 4 cut(s) 86, 241, 242, 899
SchI GAGTC 1 cut(s) 813
ScrFI CCNGG 2 cut(s) 291, 552
SduI GDGCHC 2 cut(s) 245, 971
SetI ASST 9 cut(s) 222, 343, 400, 408, 439, 475, 520, 685, 799
SfoI GGCGCC 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SpeI ACTAGT 1 cut(s) 650
SphI GCATGC 1 cut(s) 378
Sse9I AATT 5 cut(s) 45, 151, 503, 731, 972
SsiI CCGC 2 cut(s) 284, 390
SspDI GGCGCC 1 cut(s) 541
SspMI CTAG 4 cut(s) 342, 489, 537, 651
StyD4I CCNGG 2 cut(s) 289, 550
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 2 cut(s) 760, 805
TaiI ACGT 2 cut(s) 222, 408
TaqI TCGA 2 cut(s) 236, 728
TasI AATT 5 cut(s) 45, 151, 503, 731, 972
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 2 cut(s) 725, 835
Tru1I TTAA 3 cut(s) 672, 906, 994
Tru9I TTAA 3 cut(s) 672, 906, 994
TseFI GTSAC 3 cut(s) 429, 760, 886
TseI GCWGC 3 cut(s) 308, 398, 437
Tsp45I GTSAC 3 cut(s) 429, 760, 886
TspDTI ATGAA 2 cut(s) 90, 707
TspGWI ACGGA 1 cut(s) 528
Van91I CCANNNNNTGG 2 cut(s) 326, 551
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 3 cut(s) 151, 503, 731
XceI RCATGY 1 cut(s) 378
XspI CTAG 4 cut(s) 342, 489, 537, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.