Rw1G015560

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Reverse (-)
34687943 .. 34691307
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015560.1

Sequence Viewer

Length: 2175 bp
ATGAGAGCACTACCTTTTATAGGCTCTAGTAGCACCATACATACAGTTATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTCTACTTCTTGTTCATGTTACTCACTCAGCTGCTCGGGATGCCCGGAAGGTATATCTCCCAAGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGAGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGACGTAAGAAGTTTCCATGGATTTGCTGCAATGCTCACAAAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAATGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAAAGAAAATCGGATAGTTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTATTATCGTCATTTGGGATCCTTCGAAAAAGAAGTTGAGTCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCGATAGCAGCTGGGAACATAGTGAGAAACACGAGTATAAAAGGTTTAGGGTTTGGAAACGCAAGAGGAGGTGTGCCATCAGCACATATTGCAGCGTACAAAGTGTGCTGGAAGGATGGTTGTTCAGATGCTGACATTCTAGCGGCATTCGATGATGCCGTTGCTGATGGTGTCGACATAATCTCTATTTCCATTGGAAGCAAAGTTCCAACTGATTATTTTCGAAGCGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAATGGGATACTTGTTTCAACGTCCAGTGGTAACGAAGGTCCAAAAAAGAAAACTGTGTCAAACTTTGCACCGTGGATGCTTTCTGTGGCTGCTACTACCATAAATCGTCAGTTCGTCACCAAGGTTCAATTAGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATACATGACCTCCAGGGTAAATTCTATCCTTTAATATATGGTGGGGACGCACCTAACGCAACAGAAGGTTCTAACGCATCTATGTTCTGTTCCGAAAATAGCTTAAAAGAAGATTCGATCAGTGGTAAAATTGTGCTTTGTGATAGAGGCCCTGCTGGCAATGACGATGGGTATGGGGCCATAGAGGGTGATGTAATCGGAGTTATTCTGACGGGCGTAAAAGTTGCCGAGGAGCTGGTTGGCCCTCTTTCCCTGCCTGCATCTCACATCGGATTGGAAGAAAACAGCCACATTTACAAATACATAAGTGAAACAAGGAACCCAATTGCAACTATTTTCAAGAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGCCCGAATCCGATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCTCCAGGTACTCACATTTTAGCAGCATATGATCATCAATCGGTTGGTGGTGGCAGTAGAGCTAAGTATAGTTTGGAAACTGGGACATCAATGGCATGCCCCCATGCTTCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCGGCTCTCATCACTACGGCTAAACCTATGAGTACTAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCCTCCTAAACCCTTCTAGGGCTCCATATCCTGGTTTAGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATATAGTGGAAAACTATTGGAAATCATAACCAGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAACAACCAATGACCTGAACTATCCTTCTTTTGCTTTTTCAATCAAGGATCCAGAATTCATCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCGAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTCGGGCAGAAGGAATCTTTTGTTGTTACAGTAAAAGGGTCAATTGAGAAATCAAATATAGTCTCTGCATCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCGATTGTTATCCATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

724

Amino Acids

77.93

Weight (kDa)

7.6

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 83 - 115 6.3e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 179 - 567 9e-43 Subtilase family
fn3_6 PF17766 623 - 720 6.5e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 199
AccB7I CCANNNNNTGG 2 cut(s) 447, 1730
AccI GTMKAC 2 cut(s) 762, 1740
AciI CCGC 3 cut(s) 731, 816, 1673
AclWI GGATC 5 cut(s) 522, 535, 1898, 1911, 1960
AcsI RAATTY 4 cut(s) 1037, 1682, 1910, 1960
AcuI CTGAAG 2 cut(s) 585, 1551
AfaI GTAC 3 cut(s) 686, 1468, 1660
AfiI CCNNNNNNNGG 5 cut(s) 20, 447, 852, 1717, 1730
AgsI TTSAA 5 cut(s) 867, 977, 1357, 1625, 1896
AjnI CCWGG 4 cut(s) 1029, 1462, 1729, 1817
AloI GAACNNNNNNTCC 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 10, 508
Alw26I GTCTC 4 cut(s) 231, 552, 555, 2107
AlwI GGATC 5 cut(s) 522, 535, 1898, 1911, 1960
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 2 cut(s) 131, 2042
AoxI GGCC 5 cut(s) 1165, 1194, 1258, 1414, 1699
ApaI GGGCCC 1 cut(s) 1418
ApoI RAATTY 4 cut(s) 1037, 1682, 1910, 1960
AseI ATTAAT 1 cut(s) 1371
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 7 cut(s) 431, 887, 1166, 1194, 1259, 1414, 1415
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 3 cut(s) 958, 1217, 1602
AsuII TTCGAA 2 cut(s) 534, 811
AvaI CYCGRG 2 cut(s) 131, 2042
AvaII GGWCC 2 cut(s) 431, 887
BaeGI GKGCMC 2 cut(s) 1398, 1418
BamHI GGATCC 2 cut(s) 527, 1903
BanI GGYRCC 1 cut(s) 199
BanII GRGCYC 3 cut(s) 1418, 1723, 2150
BauI CACGAG 1 cut(s) 619
BbsI GAAGAC 2 cut(s) 316, 1918
Bbv12I GWGCWC 2 cut(s) 10, 508
BccI CCATC 8 cut(s) 59, 302, 673, 698, 749, 1178, 1999, 2120
BceAI ACGGC 2 cut(s) 731, 1659
BciT130I CCWGG 4 cut(s) 1031, 1464, 1731, 1819
BciVI GTATCC 1 cut(s) 849
BclI TGATCA 1 cut(s) 1489
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 4 cut(s) 231, 552, 555, 2107
BfaI CTAG 4 cut(s) 27, 728, 1668, 1716
BfuI GTATCC 1 cut(s) 849
BglI GCCNNNNNGGC 1 cut(s) 1173
BmcAI AGTACT 1 cut(s) 1660
Bme1390I CCNGG 5 cut(s) 141, 1031, 1464, 1731, 1819
Bme18I GGWCC 2 cut(s) 431, 887
BmeT110I CYCGRG 2 cut(s) 131, 2042
BmgT120I GGNCC 7 cut(s) 431, 887, 1166, 1194, 1259, 1414, 1415
BmrFI CCNGG 5 cut(s) 141, 1031, 1464, 1731, 1819
BmrI ACTGGG 1 cut(s) 1548
BmsI GCATC 8 cut(s) 126, 596, 706, 733, 915, 1103, 1286, 2117
BmuI ACTGGG 1 cut(s) 1548
BpiI GAAGAC 2 cut(s) 316, 1918
BplI GAGNNNNNCTC 2 cut(s) 1686, 1718
BpmI CTGGAG 2 cut(s) 1013, 1446
Bpu14I TTCGAA 2 cut(s) 534, 811
BpuEI CTTGAG 2 cut(s) 395, 1427
BpuMI CCSGG 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 1393
BsaBI GATNNNNATC 1 cut(s) 2156
BsaJI CCNNGG 6 cut(s) 253, 920, 969, 1030, 1245, 1818
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 1382, 1412
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 447, 852, 1717, 1730
Bse118I RCCGGY 1 cut(s) 1612
Bse1I ACTGG 4 cut(s) 203, 300, 873, 1543
Bse3DI GCAATG 3 cut(s) 273, 580, 1183
Bse8I GATNNNNATC 1 cut(s) 2156
BseBI CCWGG 4 cut(s) 1031, 1464, 1731, 1819
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 6 cut(s) 253, 920, 969, 1030, 1245, 1818
BseGI GGATG 5 cut(s) 141, 313, 709, 930, 2128
BseJI GATNNNNATC 1 cut(s) 2156
BseLI CCNNNNNNNGG 5 cut(s) 20, 447, 852, 1717, 1730
BseMI GCAATG 3 cut(s) 273, 580, 1183
BseMII CTCAG 2 cut(s) 93, 137
BseNI ACTGG 4 cut(s) 203, 300, 873, 1543
BseRI GAGGAG 2 cut(s) 669, 1262
BseSI GKGCMC 2 cut(s) 1398, 1418
BseYI CCCAGC 1 cut(s) 599
BshFI GGCC 5 cut(s) 1167, 1196, 1260, 1416, 1701
BshNI GGYRCC 1 cut(s) 199
BshVI ATCGAT 1 cut(s) 590
BsiHKAI GWGCWC 2 cut(s) 10, 508
BsiHKCI CYCGRG 2 cut(s) 131, 2042
BsiSI CCGG 2 cut(s) 141, 1613
BslFI GGGAC 4 cut(s) 1017, 1076, 1555, 1835
BslI CCNNNNNNNGG 5 cut(s) 20, 447, 852, 1717, 1730
BsmAI GTCTC 4 cut(s) 231, 552, 555, 2107
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 4 cut(s) 1017, 1076, 1555, 1835
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 5 cut(s) 1167, 1196, 1260, 1416, 1701
BsoBI CYCGRG 2 cut(s) 131, 2042
Bsp119I TTCGAA 2 cut(s) 534, 811
Bsp120I GGGCCC 1 cut(s) 1414
Bsp1286I GDGCHC 6 cut(s) 10, 508, 1398, 1418, 1723, 2150
Bsp143I GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 3 cut(s) 731, 816, 1673
BspANI GGCC 5 cut(s) 1167, 1196, 1260, 1416, 1701
BspCNI CTCAG 2 cut(s) 92, 136
BspDI ATCGAT 1 cut(s) 590
BspPI GGATC 5 cut(s) 522, 535, 1898, 1911, 1960
BspQI GCTCTTC 1 cut(s) 65
BspT104I TTCGAA 2 cut(s) 534, 811
BspT107I GGYRCC 1 cut(s) 199
BsrDI GCAATG 3 cut(s) 273, 580, 1183
BsrFI RCCGGY 1 cut(s) 1612
BsrI ACTGG 4 cut(s) 203, 300, 873, 1543
BssAI RCCGGY 1 cut(s) 1612
BssECI CCNNGG 6 cut(s) 253, 920, 969, 1030, 1245, 1818
BssMI GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
BssNAI GTATAC 1 cut(s) 1741
BssSI CACGAG 1 cut(s) 619
BssT1I CCWWGG 2 cut(s) 253, 969
Bst1107I GTATAC 1 cut(s) 1741
Bst2BI CACGAG 1 cut(s) 619
Bst2UI CCWGG 4 cut(s) 1031, 1464, 1731, 1819
Bst4CI ACNGT 9 cut(s) 46, 391, 510, 904, 921, 989, 1939, 2071, 2170
Bst6I CTCTTC 2 cut(s) 65, 1359
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 1393
BstBI TTCGAA 2 cut(s) 534, 811
BstC8I GCNNGC 6 cut(s) 1174, 1275, 1555, 1673, 1677, 1699
BstDEI CTNAG 3 cut(s) 79, 123, 1521
BstDSI CCRYGG 2 cut(s) 253, 920
BstEII GGTNACC 1 cut(s) 1608
BstENI CCTNNNNNAGG 1 cut(s) 18
BstF5I GGATG 5 cut(s) 141, 313, 709, 930, 2128
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 7 cut(s) 217, 530, 1137, 1492, 1747, 1906, 1955
BstMAI GTCTC 4 cut(s) 231, 552, 555, 2107
BstMBI GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
BstNI CCWGG 4 cut(s) 1031, 1464, 1731, 1819
BstNSI RCATGY 1 cut(s) 1557
BstPI GGTNACC 1 cut(s) 1608
BstSCI CCNGG 5 cut(s) 139, 1029, 1462, 1729, 1817
BstSLI GKGCMC 2 cut(s) 1398, 1418
BstV2I GAAGAC 2 cut(s) 316, 1918
BstX2I RGATCY 2 cut(s) 527, 1903
BstYI RGATCY 2 cut(s) 527, 1903
BstZ17I GTATAC 1 cut(s) 1741
Bsu15I ATCGAT 1 cut(s) 590
BsuI GTATCC 1 cut(s) 849
BsuRI GGCC 5 cut(s) 1167, 1196, 1260, 1416, 1701
BsuTUI ATCGAT 1 cut(s) 590
BtgI CCRYGG 2 cut(s) 253, 920
BtsCI GGATG 5 cut(s) 141, 313, 709, 930, 2128
BtsIMutI CAGTG 2 cut(s) 880, 1144
Cac8I GCNNGC 6 cut(s) 1174, 1275, 1555, 1673, 1677, 1699
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 1 cut(s) 587
Cfr10I RCCGGY 1 cut(s) 1612
Cfr13I GGNCC 7 cut(s) 431, 887, 1166, 1194, 1259, 1414, 1415
ClaI ATCGAT 1 cut(s) 590
CseI GACGC 2 cut(s) 334, 1073
Csp6I GTAC 3 cut(s) 685, 1467, 1659
CviAII CATG 9 cut(s) 112, 221, 254, 363, 572, 1022, 1554, 1562, 2162
CviQI GTAC 3 cut(s) 685, 1467, 1659
DdeI CTNAG 3 cut(s) 79, 123, 1521
DpnI GATC 7 cut(s) 216, 529, 1136, 1491, 1746, 1905, 1954
DpnII GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
Eam1104I CTCTTC 2 cut(s) 65, 1359
EarI CTCTTC 2 cut(s) 65, 1359
Eco130I CCWWGG 2 cut(s) 253, 969
Eco24I GRGCYC 3 cut(s) 1418, 1723, 2150
Eco47I GGWCC 2 cut(s) 431, 887
Eco57I CTGAAG 2 cut(s) 585, 1551
Eco88I CYCGRG 2 cut(s) 131, 2042
Eco91I GGTNACC 1 cut(s) 1608
EcoNI CCTNNNNNAGG 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 1166, 1414
EcoO65I GGTNACC 1 cut(s) 1608
EcoRI GAATTC 2 cut(s) 1910, 1960
EcoRII CCWGG 4 cut(s) 1029, 1462, 1729, 1817
EcoT14I CCWWGG 2 cut(s) 253, 969
EcoT38I GRGCYC 3 cut(s) 1418, 1723, 2150
ErhI CCWWGG 2 cut(s) 253, 969
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 9 cut(s) 115, 224, 257, 366, 575, 1025, 1557, 1565, 2165
FaqI GGGAC 4 cut(s) 1017, 1076, 1555, 1835
FatI CATG 9 cut(s) 111, 220, 253, 362, 571, 1021, 1553, 1561, 2161
FauI CCCGC 1 cut(s) 1680
FauNDI CATATG 3 cut(s) 458, 1486, 1690
FbaI TGATCA 1 cut(s) 1489
FblI GTMKAC 2 cut(s) 762, 1740
FokI GGATG 5 cut(s) 148, 320, 716, 937, 2135
FriOI GRGCYC 3 cut(s) 1418, 1723, 2150
FspBI CTAG 4 cut(s) 27, 728, 1668, 1716
FspI TGCGCA 1 cut(s) 586
GlaI GCGC 1 cut(s) 586
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 2 cut(s) 1013, 1446
HaeIII GGCC 5 cut(s) 1167, 1196, 1260, 1416, 1701
HapII CCGG 2 cut(s) 141, 1613
HgaI GACGC 2 cut(s) 334, 1073
HhaI GCGC 1 cut(s) 587
Hin1II CATG 9 cut(s) 115, 224, 257, 366, 575, 1025, 1557, 1565, 2165
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 8 cut(s) 548, 560, 1130, 1420, 1429, 1998, 2014, 2054
HpaII CCGG 2 cut(s) 141, 1613
HphI GGTGA 3 cut(s) 958, 1217, 1602
Hpy166II GTNNAC 3 cut(s) 763, 1741, 1781
Hpy188I TCNGA 9 cut(s) 406, 503, 565, 715, 1111, 1217, 1227, 1289, 1425
Hpy188III TCNNGA 7 cut(s) 133, 383, 1357, 1748, 1907, 1995, 2143
Hpy8I GTNNAC 3 cut(s) 763, 1741, 1781
Hpy99I CGWCG 1 cut(s) 1960
HpyCH4III ACNGT 9 cut(s) 46, 391, 510, 904, 921, 989, 1939, 2071, 2170
HpyCH4IV ACGT 4 cut(s) 240, 869, 1392, 1584
HpyCH4V TGCA 9 cut(s) 266, 680, 824, 917, 1277, 1346, 1580, 1688, 2108
HpyF3I CTNAG 3 cut(s) 79, 123, 1521
HpySE526I ACGT 4 cut(s) 240, 869, 1392, 1584
Hsp92II CATG 9 cut(s) 115, 224, 257, 366, 575, 1025, 1557, 1565, 2165
HspAI GCGC 1 cut(s) 585
Ksp22I TGATCA 1 cut(s) 1489
Kzo9I GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 8 cut(s) 503, 1249, 1390, 1465, 1694, 1726, 1993, 2145
LweI GCATC 8 cut(s) 126, 596, 706, 733, 915, 1103, 1286, 2117
MaeI CTAG 4 cut(s) 27, 728, 1668, 1716
MaeII ACGT 4 cut(s) 240, 869, 1392, 1584
MalI GATC 7 cut(s) 216, 529, 1136, 1491, 1746, 1905, 1954
MboI GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
MboII GAAGA 7 cut(s) 52, 209, 316, 1139, 1307, 1376, 1918
MfeI CAATTG 3 cut(s) 825, 1341, 2082
MflI RGATCY 2 cut(s) 527, 1903
MhlI GDGCHC 6 cut(s) 10, 508, 1398, 1418, 1723, 2150
MlyI GAGTC 3 cut(s) 554, 557, 1992
MmeI TCCRAC 2 cut(s) 821, 1930
MseI TTAA 5 cut(s) 393, 1049, 1121, 1371, 2173
MspA1I CMGCKG 2 cut(s) 127, 599
MspI CCGG 2 cut(s) 141, 1613
MspR9I CCNGG 5 cut(s) 141, 1031, 1464, 1731, 1819
MunI CAATTG 3 cut(s) 825, 1341, 2082
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 4 cut(s) 1031, 1464, 1731, 1819
NciI CCSGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 253
NdeI CATATG 3 cut(s) 458, 1486, 1690
NdeII GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
NlaIII CATG 9 cut(s) 115, 224, 257, 366, 575, 1025, 1557, 1565, 2165
NmeAIII GCCGAG 1 cut(s) 1270
NmuCI GTSAC 3 cut(s) 964, 1608, 1939
NsbI TGCGCA 1 cut(s) 586
NspI RCATGY 1 cut(s) 1557
NspV TTCGAA 2 cut(s) 534, 811
PaeI GCATGC 1 cut(s) 1557
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 514, 744
PctI GAATGC 1 cut(s) 734
PfeI GAWTC 5 cut(s) 1130, 1420, 1429, 2014, 2054
PflMI CCANNNNNTGG 2 cut(s) 447, 1730
PleI GAGTC 3 cut(s) 554, 556, 1992
PpsI GAGTC 3 cut(s) 554, 556, 1992
Ppu21I YACGTR 1 cut(s) 1393
PshBI ATTAAT 1 cut(s) 1371
Psp6I CCWGG 4 cut(s) 1029, 1462, 1729, 1817
PspEI GGTNACC 1 cut(s) 1608
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 4 cut(s) 1029, 1462, 1729, 1817
PspOMI GGGCCC 1 cut(s) 1414
PspPI GGNCC 7 cut(s) 431, 887, 1166, 1194, 1259, 1414, 1415
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 2 cut(s) 527, 1903
PvuII CAGCTG 2 cut(s) 127, 599
RsaI GTAC 3 cut(s) 686, 1468, 1660
RsaNI GTAC 3 cut(s) 685, 1467, 1659
SalI GTCGAC 1 cut(s) 761
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 5 cut(s) 393, 1049, 1121, 1371, 2173
Sau3AI GATC 7 cut(s) 214, 527, 1134, 1489, 1744, 1903, 1952
Sau96I GGNCC 7 cut(s) 431, 887, 1166, 1194, 1259, 1414, 1415
ScaI AGTACT 1 cut(s) 1660
SchI GAGTC 3 cut(s) 554, 557, 1992
ScrFI CCNGG 5 cut(s) 141, 1031, 1464, 1731, 1819
SduI GDGCHC 6 cut(s) 10, 508, 1398, 1418, 1723, 2150
SfaNI GCATC 8 cut(s) 126, 596, 706, 733, 915, 1103, 1286, 2117
SfuI TTCGAA 2 cut(s) 534, 811
SinI GGWCC 2 cut(s) 431, 887
SmlI CTYRAG 2 cut(s) 374, 1442
SmoI CTYRAG 2 cut(s) 374, 1442
SphI GCATGC 1 cut(s) 1557
SsiI CCGC 3 cut(s) 731, 816, 1673
SspI AATATT 1 cut(s) 229
SspMI CTAG 4 cut(s) 27, 728, 1668, 1716
StyD4I CCNGG 5 cut(s) 139, 1029, 1462, 1729, 1817
StyI CCWWGG 2 cut(s) 253, 969
TaaI ACNGT 9 cut(s) 46, 391, 510, 904, 921, 989, 1939, 2071, 2170
TaiI ACGT 4 cut(s) 243, 872, 1395, 1587
TaqI TCGA 9 cut(s) 63, 534, 590, 738, 762, 811, 1133, 1958, 2001
TatI WGTACW 1 cut(s) 1658
TauI GCSGC 1 cut(s) 734
TfiI GAWTC 5 cut(s) 1130, 1420, 1429, 2014, 2054
Tru1I TTAA 5 cut(s) 393, 1049, 1121, 1371, 2173
Tru9I TTAA 5 cut(s) 393, 1049, 1121, 1371, 2173
TscAI CASTG 2 cut(s) 880, 1144
TseFI GTSAC 3 cut(s) 964, 1608, 1939
Tsp45I GTSAC 3 cut(s) 964, 1608, 1939
TspDTI ATGAA 6 cut(s) 100, 209, 1442, 1767, 1869, 1903
TspRI CASTG 2 cut(s) 880, 1144
Van91I CCANNNNNTGG 2 cut(s) 447, 1730
VpaK11BI GGWCC 2 cut(s) 431, 887
VspI ATTAAT 1 cut(s) 1371
XagI CCTNNNNNAGG 1 cut(s) 18
XapI RAATTY 4 cut(s) 1037, 1682, 1910, 1960
XceI RCATGY 1 cut(s) 1557
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XmiI GTMKAC 2 cut(s) 762, 1740
XspI CTAG 4 cut(s) 27, 728, 1668, 1716
ZrmI AGTACT 1 cut(s) 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.