RLG00000027151

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Forward (+)
9039819 .. 9045207
5389 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027151

Sequence Viewer

Length: 2223 bp
ATGGCTCTTCGATGGTTTCTCTTTCTCAGCCTCATTTGTAGTATTCTACTGCTTGTTGTCGATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGATAAGCCAAAGAACGGGGTACCCATATTACCTAATCTTCATGTGAATATGCTACAAGACGTAGTTGACAATAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTTCTGCAATGCTCATAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAGAGGAGCGTCAAAACAACAATGTCATGGAACTTTCTTGGGTTTCCAGAAACGGTTAAGAGAGGCGCCCTTGAGAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGTTGGTTTTGGTCCCCGACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAATATTATCGCGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACTGCATCAACAGCAGCTGGGAACACAGTGATCAAGGCGAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGTATGTTGGGGTAGCAGTGGAAGTTGTGACGATGCTGATGTTCTAGCCGCATTCGATGATGCCATTGCTGATGGTGTAGACATTCTCTCTGTTTCGGTCGGGGGCACTATTCCACTTGATTATTTGAATGATCCATTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACAGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAATCGCCAATTCACCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTACCCTTTAATTTATGGTGGAGACGCGCCCAATACAAAAGCAGGTGCTGACGGGAGTATCTCTAGGTTTTGTGGAAGAGATGACTTAGACAAAGATTTGATCAAAGGTAAAATTGTGCTATGTGATGGCCCTATGGATGGGGCTGGGGTTGGGGCCATATTGGGTGGTGCAGCCGGAATTGTTCTAACGGGAAAAAAAGTCAACGATGAACTGCTTGGCCCTTATCCCGTGCCTGCATCTTTCATCGGGTTGGAAGAAAACAGCGACATTTATAAATACATAAGTTCAACAAGGAACGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATATGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCGGTAGAGCAGGATACTATATAGATACCGGGACATCAATGGCATGCCCCCATGCTACGGCCACAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCAGCGGCTGCTATCCAATCAGCTCTCATCACTACTGCTAAACCCATGAGTGCCACAACTAGCCCTAACGCTGAATTTGCATACGGAGCGGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAAGGACAAAACTAGTTGCTCATCAAAATCCATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATTTCTCGGGCAAAAGAAATCTTTTATTGTCACTATAAAAGGGCCGATTGAGAAATCAAATCTAGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

741

Amino Acids

78.89

Weight (kDa)

8.43

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 113 6.7e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 583 2.1e-41 Subtilase family
fn3_6 PF17766 639 - 736 8e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1368
AarI CACCTGC 1 cut(s) 1127
Acc36I ACCTGC 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 4 cut(s) 136, 395, 709, 1768
AccB7I CCANNNNNTGG 3 cut(s) 495, 1553, 1778
AccBSI CCGCTC 1 cut(s) 1745
AccI GTMKAC 1 cut(s) 816
AccII CGCG 3 cut(s) 567, 721, 1121
AciI CCGC 6 cut(s) 786, 927, 1511, 1560, 1661, 1745
AclWI GGATC 4 cut(s) 863, 1946, 1959, 2008
AcoI YGGCCR 3 cut(s) 443, 924, 1617
AcsI RAATTY 4 cut(s) 438, 1091, 1730, 1958
AcyI GRCGYC 2 cut(s) 396, 1769
AfaI GTAC 2 cut(s) 138, 734
AgsI TTSAA 5 cut(s) 247, 583, 865, 1031, 1383
AhlI ACTAGT 1 cut(s) 1877
AjnI CCWGG 2 cut(s) 1516, 1777
AluBI AGCT 9 cut(s) 77, 231, 459, 647, 672, 1625, 1679, 1910, 2024
AluI AGCT 9 cut(s) 77, 231, 459, 647, 672, 1625, 1679, 1910, 2024
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 4 cut(s) 600, 603, 1110, 2155
AlwI GGATC 4 cut(s) 863, 1946, 1959, 2008
AlwNI CAGNNNCTG 3 cut(s) 647, 1142, 1664
Ama87I CYCGRG 1 cut(s) 2090
AoxI GGCC 9 cut(s) 443, 924, 1222, 1248, 1312, 1468, 1617, 1747, 2126
ApaI GGGCCC 1 cut(s) 1472
ApeKI GCWGC 7 cut(s) 77, 644, 992, 1265, 1535, 1625, 1664
ApoI RAATTY 4 cut(s) 438, 1091, 1730, 1958
AseI ATTAAT 1 cut(s) 1899
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 5 cut(s) 398, 569, 721, 1123, 1771
AspS9I GGNCC 9 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 1747, 2126
AsuC2I CCSGG 3 cut(s) 945, 1085, 1588
AsuHPI GGTGA 2 cut(s) 1009, 1637
AvaI CYCGRG 1 cut(s) 2090
AvaII GGWCC 2 cut(s) 479, 941
BaeGI GKGCMC 3 cut(s) 845, 1452, 1472
BaeI ACNNNNGTAYC 4 cut(s) 1135, 1168, 1828, 1861
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 1 cut(s) 1951
BanI GGYRCC 4 cut(s) 136, 395, 709, 1768
BanII GRGCYC 2 cut(s) 1472, 2198
BbsI GAAGAC 2 cut(s) 310, 1966
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 7 cut(s) 64, 656, 979, 1277, 1547, 1612, 1651
BccI CCATC 8 cut(s) 6, 296, 721, 803, 1214, 1226, 1952, 2168
BceAI ACGGC 1 cut(s) 1632
BciT130I CCWGG 2 cut(s) 1518, 1779
BciVI GTATCC 2 cut(s) 1565, 1829
BclI TGATCA 2 cut(s) 660, 1194
BcnI CCSGG 3 cut(s) 945, 1085, 1588
BcoDI GTCTC 4 cut(s) 600, 603, 1110, 2155
BcuI ACTAGT 1 cut(s) 1877
BfaI CTAG 6 cut(s) 782, 1158, 1716, 1764, 1878, 2147
BfoI RGCGCY 2 cut(s) 399, 1772
BfuAI ACCTGC 1 cut(s) 1127
BfuI GTATCC 2 cut(s) 1565, 1829
BglI GCCNNNNNGGC 1 cut(s) 95
BglII AGATCT 1 cut(s) 214
Bme1390I CCNGG 5 cut(s) 945, 1085, 1518, 1588, 1779
Bme18I GGWCC 2 cut(s) 479, 941
BmeT110I CYCGRG 1 cut(s) 2090
BmgT120I GGNCC 9 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 1747, 2126
BmrFI CCNGG 5 cut(s) 945, 1085, 1518, 1588, 1779
BmsI GCATC 5 cut(s) 76, 644, 760, 787, 1340
BpiI GAAGAC 2 cut(s) 310, 1966
BpmI CTGGAG 1 cut(s) 1500
Bpu10I CCTNAGC 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 422, 1481
BpuMI CCSGG 3 cut(s) 945, 1085, 1588
BsaHI GRCGYC 2 cut(s) 396, 1769
BsaJI CCNNGG 5 cut(s) 921, 974, 1023, 1084, 1866
BsaXI ACNNNNNCTCC 2 cut(s) 2020, 2050
Bse3DI GCAATG 3 cut(s) 267, 523, 801
BseBI CCWGG 2 cut(s) 1518, 1779
BseDI CCNNGG 5 cut(s) 921, 974, 1023, 1084, 1866
BseGI GGATG 4 cut(s) 91, 307, 1237, 2176
BseMI GCAATG 3 cut(s) 267, 523, 801
BseMII CTCAG 3 cut(s) 40, 87, 95
BseRI GAGGAG 2 cut(s) 349, 717
BseSI GKGCMC 3 cut(s) 845, 1452, 1472
BseXI GCAGC 7 cut(s) 64, 656, 979, 1277, 1547, 1612, 1651
BseYI CCCAGC 2 cut(s) 647, 1238
BsgI GTGCAG 1 cut(s) 1284
Bsh1236I CGCG 3 cut(s) 567, 721, 1121
Bsh1285I CGRYCG 1 cut(s) 837
BshFI GGCC 9 cut(s) 445, 926, 1224, 1250, 1314, 1470, 1619, 1749, 2128
BshNI GGYRCC 4 cut(s) 136, 395, 709, 1768
BsiEI CGRYCG 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 2090
BsiSI CCGG 4 cut(s) 944, 1084, 1269, 1587
BslFI GGGAC 4 cut(s) 465, 1071, 1603, 2058
BsmAI GTCTC 4 cut(s) 600, 603, 1110, 2155
BsmBI CGTCTC 1 cut(s) 1110
BsmFI GGGAC 4 cut(s) 465, 1071, 1603, 2058
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 9 cut(s) 445, 926, 1224, 1250, 1314, 1470, 1619, 1749, 2128
BsoBI CYCGRG 1 cut(s) 2090
Bsp120I GGGCCC 1 cut(s) 1468
Bsp1286I GDGCHC 5 cut(s) 556, 845, 1452, 1472, 2198
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
Bsp19I CCATGG 2 cut(s) 921, 974
BspACI CCGC 6 cut(s) 786, 927, 1511, 1560, 1661, 1745
BspANI GGCC 9 cut(s) 445, 926, 1224, 1250, 1314, 1470, 1619, 1749, 2128
BspCNI CTCAG 3 cut(s) 39, 86, 94
BspFNI CGCG 3 cut(s) 567, 721, 1121
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 4 cut(s) 863, 1946, 1959, 2008
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 4 cut(s) 136, 395, 709, 1768
BsrBI CCGCTC 1 cut(s) 1745
BsrDI GCAATG 3 cut(s) 267, 523, 801
BsrGI TGTACA 1 cut(s) 732
BssECI CCNNGG 5 cut(s) 921, 974, 1023, 1084, 1866
BssMI GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
BssNI GRCGYC 2 cut(s) 396, 1769
BssT1I CCWWGG 4 cut(s) 921, 974, 1023, 1866
Bst2UI CCWGG 2 cut(s) 1518, 1779
Bst4CI ACNGT 8 cut(s) 385, 434, 617, 658, 958, 1841, 1987, 2032
Bst6I CTCTTC 3 cut(s) 12, 1165, 1413
BstACI GRCGYC 2 cut(s) 396, 1769
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 5 cut(s) 670, 1329, 1603, 1747, 1857
BstDEI CTNAG 4 cut(s) 26, 73, 81, 1180
BstDSI CCRYGG 2 cut(s) 921, 974
BstF5I GGATG 4 cut(s) 91, 307, 1237, 2176
BstFNI CGCG 3 cut(s) 567, 721, 1121
BstH2I RGCGCY 2 cut(s) 399, 1772
BstHHI GCGC 5 cut(s) 398, 569, 721, 1123, 1771
BstKTI GATC 8 cut(s) 217, 663, 871, 1197, 1482, 1795, 1954, 2003
BstMAI GTCTC 4 cut(s) 600, 603, 1110, 2155
BstMBI GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
BstMCI CGRYCG 1 cut(s) 837
BstMWI GCNNNNNNNGC 9 cut(s) 86, 95, 271, 641, 718, 725, 1625, 1733, 1742
BstNI CCWGG 2 cut(s) 1518, 1779
BstNSI RCATGY 1 cut(s) 1605
BstSCI CCNGG 5 cut(s) 943, 1083, 1516, 1586, 1777
BstSLI GKGCMC 3 cut(s) 845, 1452, 1472
BstUI CGCG 3 cut(s) 567, 721, 1121
BstV1I GCAGC 7 cut(s) 64, 656, 979, 1277, 1547, 1612, 1651
BstV2I GAAGAC 2 cut(s) 310, 1966
BstX2I RGATCY 2 cut(s) 214, 1951
BstYI RGATCY 2 cut(s) 214, 1951
BsuI GTATCC 2 cut(s) 1565, 1829
BsuRI GGCC 9 cut(s) 445, 926, 1224, 1250, 1314, 1470, 1619, 1749, 2128
BtgI CCRYGG 2 cut(s) 921, 974
BtsCI GGATG 4 cut(s) 91, 307, 1237, 2176
BtsI GCAGTG 2 cut(s) 630, 760
BtsIMutI CAGTG 6 cut(s) 439, 630, 663, 760, 963, 1846
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 5 cut(s) 670, 1329, 1603, 1747, 1857
CaiI CAGNNNCTG 3 cut(s) 647, 1142, 1664
CfoI GCGC 5 cut(s) 398, 569, 721, 1123, 1771
Cfr13I GGNCC 9 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 1747, 2126
CseI GACGC 3 cut(s) 328, 1127, 2145
Csp6I GTAC 2 cut(s) 137, 733
CviQI GTAC 2 cut(s) 137, 733
DdeI CTNAG 4 cut(s) 26, 73, 81, 1180
DinI GGCGCC 2 cut(s) 397, 1770
DpnI GATC 8 cut(s) 216, 662, 870, 1196, 1481, 1794, 1953, 2002
DpnII GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
EaeI YGGCCR 3 cut(s) 443, 924, 1617
Eam1104I CTCTTC 3 cut(s) 12, 1165, 1413
EarI CTCTTC 3 cut(s) 12, 1165, 1413
Eco130I CCWWGG 4 cut(s) 921, 974, 1023, 1866
Eco24I GRGCYC 2 cut(s) 1472, 2198
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 479, 941
Eco88I CYCGRG 1 cut(s) 2090
EcoO109I RGGNCCY 1 cut(s) 1468
EcoRII CCWGG 2 cut(s) 1516, 1777
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 4 cut(s) 921, 974, 1023, 1866
EcoT38I GRGCYC 2 cut(s) 1472, 2198
EgeI GGCGCC 2 cut(s) 397, 1770
EheI GGCGCC 2 cut(s) 397, 1770
ErhI CCWWGG 4 cut(s) 921, 974, 1023, 1866
Esp3I CGTCTC 1 cut(s) 1110
FalI AAGNNNNNCTT 4 cut(s) 204, 236, 656, 688
FaqI GGGAC 4 cut(s) 465, 1071, 1603, 2058
FauI CCCGC 1 cut(s) 1738
FauNDI CATATG 2 cut(s) 1444, 1630
FbaI TGATCA 2 cut(s) 660, 1194
FblI GTMKAC 1 cut(s) 816
FokI GGATG 4 cut(s) 98, 314, 1244, 2183
FriOI GRGCYC 2 cut(s) 1472, 2198
FspBI CTAG 6 cut(s) 782, 1158, 1716, 1764, 1878, 2147
GlaI GCGC 5 cut(s) 397, 568, 720, 1122, 1770
GsaI CCCAGC 2 cut(s) 651, 1242
GsuI CTGGAG 1 cut(s) 1500
HaeII RGCGCY 2 cut(s) 399, 1772
HaeIII GGCC 9 cut(s) 445, 926, 1224, 1250, 1314, 1470, 1619, 1749, 2128
HapII CCGG 4 cut(s) 944, 1084, 1269, 1587
HgaI GACGC 3 cut(s) 328, 1127, 2145
HhaI GCGC 5 cut(s) 398, 569, 721, 1123, 1771
Hin1I GRCGYC 2 cut(s) 396, 1769
Hin6I GCGC 5 cut(s) 396, 567, 719, 1121, 1769
HinP1I GCGC 5 cut(s) 396, 567, 719, 1121, 1769
HincII GTYRAC 2 cut(s) 184, 1297
HindII GTYRAC 2 cut(s) 184, 1297
HinfI GANTC 5 cut(s) 449, 596, 608, 2046, 2062
HpaII CCGG 4 cut(s) 944, 1084, 1269, 1587
HphI GGTGA 2 cut(s) 1009, 1637
Hpy166II GTNNAC 7 cut(s) 184, 733, 817, 1297, 1829, 1847, 2082
Hpy188I TCNGA 4 cut(s) 454, 551, 1479, 1956
Hpy188III TCNNGA 5 cut(s) 83, 377, 690, 1796, 2191
Hpy8I GTNNAC 7 cut(s) 184, 733, 817, 1297, 1829, 1847, 2082
HpyAV CCTTC 3 cut(s) 932, 1770, 1938
HpyCH4III ACNGT 8 cut(s) 385, 434, 617, 658, 958, 1841, 1987, 2032
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 9 cut(s) 86, 95, 271, 641, 718, 725, 1625, 1733, 1742
HpyF3I CTNAG 4 cut(s) 26, 73, 81, 1180
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 2 cut(s) 396, 1769
HspAI GCGC 5 cut(s) 396, 567, 719, 1121, 1769
KasI GGCGCC 2 cut(s) 395, 1768
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 2 cut(s) 660, 1194
Kzo9I GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 8 cut(s) 336, 551, 947, 1444, 1519, 1742, 2041, 2193
Lsp1109I GCAGC 7 cut(s) 64, 656, 979, 1277, 1547, 1612, 1651
LweI GCATC 5 cut(s) 76, 644, 760, 787, 1340
MaeI CTAG 6 cut(s) 782, 1158, 1716, 1764, 1878, 2147
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 7 cut(s) 64, 533, 764, 932, 958, 1987, 2113
MalI GATC 8 cut(s) 216, 662, 870, 1196, 1481, 1794, 1953, 2002
MbiI CCGCTC 1 cut(s) 1745
MboI GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
MboII GAAGA 9 cut(s) 146, 215, 224, 310, 599, 1182, 1361, 1430, 1966
MfeI CAATTG 2 cut(s) 879, 1031
MflI RGATCY 2 cut(s) 214, 1951
MhlI GDGCHC 5 cut(s) 556, 845, 1452, 1472, 2198
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
Mly113I GGCGCC 2 cut(s) 396, 1769
MlyI GAGTC 4 cut(s) 458, 602, 605, 2040
MmeI TCCRAC 3 cut(s) 432, 1326, 1978
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 5 cut(s) 387, 675, 1103, 1899, 2221
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 5 cut(s) 77, 647, 929, 1625, 1661
MspI CCGG 4 cut(s) 944, 1084, 1269, 1587
MspR9I CCNGG 5 cut(s) 945, 1085, 1518, 1588, 1779
MunI CAATTG 2 cut(s) 879, 1031
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 2 cut(s) 1518, 1779
MvnI CGCG 3 cut(s) 567, 721, 1121
MwoI GCNNNNNNNGC 9 cut(s) 86, 95, 271, 641, 718, 725, 1625, 1733, 1742
NarI GGCGCC 2 cut(s) 396, 1769
NciI CCSGG 3 cut(s) 945, 1085, 1588
NcoI CCATGG 2 cut(s) 921, 974
NdeI CATATG 2 cut(s) 1444, 1630
NdeII GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
NmuCI GTSAC 4 cut(s) 764, 958, 1987, 2113
NspI RCATGY 1 cut(s) 1605
PaeI GCATGC 1 cut(s) 1605
PaqCI CACCTGC 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 2062
PflMI CCANNNNNTGG 3 cut(s) 495, 1553, 1778
PfoI TCCNGGA 2 cut(s) 943, 1516
PleI GAGTC 4 cut(s) 457, 602, 604, 2040
PluTI GGCGCC 2 cut(s) 399, 1772
PpsI GAGTC 4 cut(s) 457, 602, 604, 2040
PshBI ATTAAT 1 cut(s) 1899
PsiI TTATAA 1 cut(s) 1368
Psp6I CCWGG 2 cut(s) 1516, 1777
PspFI CCCAGC 2 cut(s) 647, 1238
PspGI CCWGG 2 cut(s) 1516, 1777
PspOMI GGGCCC 1 cut(s) 1468
PspPI GGNCC 9 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 1747, 2126
PstNI CAGNNNCTG 3 cut(s) 647, 1142, 1664
PsuI RGATCY 2 cut(s) 214, 1951
PvuII CAGCTG 3 cut(s) 77, 647, 1625
RsaI GTAC 2 cut(s) 138, 734
RsaNI GTAC 2 cut(s) 137, 733
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 5 cut(s) 387, 675, 1103, 1899, 2221
Sau3AI GATC 8 cut(s) 214, 660, 868, 1194, 1479, 1792, 1951, 2000
Sau96I GGNCC 9 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 1747, 2126
SchI GAGTC 4 cut(s) 458, 602, 605, 2040
ScrFI CCNGG 5 cut(s) 945, 1085, 1518, 1588, 1779
SduI GDGCHC 5 cut(s) 556, 845, 1452, 1472, 2198
SfaNI GCATC 5 cut(s) 76, 644, 760, 787, 1340
SfoI GGCGCC 2 cut(s) 397, 1770
SinI GGWCC 2 cut(s) 479, 941
SmlI CTYRAG 2 cut(s) 401, 1496
SmoI CTYRAG 2 cut(s) 401, 1496
SpeI ACTAGT 1 cut(s) 1877
SphI GCATGC 1 cut(s) 1605
SsiI CCGC 6 cut(s) 786, 927, 1511, 1560, 1661, 1745
SspDI GGCGCC 2 cut(s) 395, 1768
SspI AATATT 1 cut(s) 560
SspMI CTAG 6 cut(s) 782, 1158, 1716, 1764, 1878, 2147
StyD4I CCNGG 5 cut(s) 943, 1083, 1516, 1586, 1777
StyI CCWWGG 4 cut(s) 921, 974, 1023, 1866
TaaI ACNGT 8 cut(s) 385, 434, 617, 658, 958, 1841, 1987, 2032
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 10, 60, 792, 1463
TaqII GACCGA 1 cut(s) 823
TatI WGTACW 1 cut(s) 732
TauI GCSGC 4 cut(s) 788, 929, 1514, 1664
TfiI GAWTC 1 cut(s) 2062
Tru1I TTAA 5 cut(s) 387, 675, 1103, 1899, 2221
Tru9I TTAA 5 cut(s) 387, 675, 1103, 1899, 2221
TscAI CASTG 6 cut(s) 439, 637, 663, 760, 963, 1846
TseFI GTSAC 4 cut(s) 764, 958, 1987, 2113
TseI GCWGC 7 cut(s) 77, 644, 992, 1265, 1535, 1625, 1664
Tsp45I GTSAC 4 cut(s) 764, 958, 1987, 2113
TspDTI ATGAA 6 cut(s) 146, 225, 1317, 1327, 1815, 1934
TspGWI ACGGA 1 cut(s) 1755
TspRI CASTG 6 cut(s) 439, 637, 663, 760, 963, 1846
Van91I CCANNNNNTGG 3 cut(s) 495, 1553, 1778
VpaK11BI GGWCC 2 cut(s) 479, 941
VspI ATTAAT 1 cut(s) 1899
XapI RAATTY 4 cut(s) 438, 1091, 1730, 1958
XceI RCATGY 1 cut(s) 1605
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 6 cut(s) 782, 1158, 1716, 1764, 1878, 2147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.