RchiOBHm_Chr1g0344291

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
36601228 .. 36603128
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57075

Sequence Viewer

Length: 1305 bp
ATGCAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTAAGAGACATAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAAAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAATGAGTGTCAAAACAACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCAACCTTGAAAAAGATATCATTATCGGTGTGATCGACACTGGAATTTGGCCAGAATCGCACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGATATTACCGTCATTTGGGATCCTTTGAAGAAGAAGTTGAGTCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCAATAGCAGTTGGGAACATAGTGAGAAATATAAGCATAAAAGGTTTAGGGTTTGGAAACGCAAGAGGAGGGGTGCCATCAGCACATATTGCGGTGTACAAAGCATGCTGGAAGAACGGTTGTTCAGATGCTGACATTCTAGCAGCATTTGATGATGCCATTGCTGACGGTGTCGATATAATCTCGGCTTCCATTGCAAGCAAAGTTCCAAATGATTATTTTCGAAGCGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAAGGGGATACTAGTTTCAACTTGCAGTGGTAACGAAGGTCCGAAAAAAAGAACTGTGATAAACTTTGCACCGTGGATGCTTTCTGTGGCTGCTACCACCATAAACCGTCAGTTCATCACCAAGGTTCAATTGGGTAATGGAAAAGTCTATGAGGGACTATTACCAAACATACATGACCTCCAGGGTAAATTCTATCCTTTAATATATGGTGGAGACGCACCTAATACAACAGAAGGTGGTAACGCGACTATGTCAAGGTTCTGTTCCACAAATAGCTTAAAAGATGATTCGATCAGAGGTAAAATTGTGCTTTGCGATAGAGGCCCAGATGCCAGTGGGGATGGGTATGGGGCCATAGAGGGTGATGCAACTGGAGTTATTCTGACGGGCGTAAAAGTTGTCGAGGAGCTGGTTGGCCCTTTTCCCCTGCCTGCAACTCACGTCGGATTGGAAGAAAACAGCAAAATTTACAAATACATAAGCGAAACAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

434

Amino Acids

46.9

Weight (kDa)

7.17

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 4 - 84 4.9e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 107 - 346 1.3e-22 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 547
AccB1I GGYRCC 2 cut(s) 46, 622
AccB7I CCANNNNNTGG 1 cut(s) 408
AccII CGCG 1 cut(s) 1055
AciI CCGC 2 cut(s) 641, 777
AclWI GGATC 2 cut(s) 483, 496
AcoI YGGCCR 1 cut(s) 356
AcsI RAATTY 3 cut(s) 351, 998, 1275
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 4 cut(s) 41, 408, 484, 813
AgsI TTSAA 4 cut(s) 317, 497, 828, 938
AhlI ACTAGT 1 cut(s) 820
AjiI CACGTC 1 cut(s) 1252
AjnI CCWGG 1 cut(s) 990
AluBI AGCT 5 cut(s) 128, 144, 372, 1086, 1219
AluI AGCT 5 cut(s) 128, 144, 372, 1086, 1219
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 4 cut(s) 78, 513, 516, 1017
AlwI GGATC 2 cut(s) 483, 496
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 4 cut(s) 356, 1132, 1161, 1225
ApeKI GCWGC 3 cut(s) 170, 692, 899
ApoI RAATTY 3 cut(s) 351, 998, 1275
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 5 cut(s) 392, 848, 1133, 1161, 1226
AsuHPI GGTGA 2 cut(s) 919, 1184
AsuII TTCGAA 1 cut(s) 772
AvaII GGWCC 2 cut(s) 392, 848
BalI TGGCCA 1 cut(s) 358
BamHI GGATCC 1 cut(s) 488
BanI GGYRCC 2 cut(s) 46, 622
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 3 cut(s) 157, 704, 886
BccI CCATC 3 cut(s) 209, 634, 1145
BciT130I CCWGG 1 cut(s) 992
BciVI GTATCC 1 cut(s) 810
BcoDI GTCTC 4 cut(s) 78, 513, 516, 1017
BcuI ACTAGT 1 cut(s) 820
BfaI CTAG 2 cut(s) 689, 821
BfuI GTATCC 1 cut(s) 810
BisI GCNGC 3 cut(s) 171, 693, 900
BlsI GCNGC 3 cut(s) 172, 694, 901
Bme1390I CCNGG 1 cut(s) 992
Bme18I GGWCC 2 cut(s) 392, 848
BmgBI CACGTC 1 cut(s) 1252
BmgT120I GGNCC 5 cut(s) 392, 848, 1133, 1161, 1226
BmiI GGNNCC 5 cut(s) 48, 490, 538, 624, 1162
BmrFI CCNGG 1 cut(s) 992
BmsI GCATC 6 cut(s) 557, 667, 694, 876, 1129, 1165
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 2 cut(s) 974, 1203
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 160, 881, 930, 991
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 408, 484, 813
Bse1I ACTGG 5 cut(s) 50, 207, 352, 1143, 1186
Bse3DI GCAATG 4 cut(s) 180, 541, 708, 741
BseBI CCWGG 1 cut(s) 992
BseDI CCNNGG 4 cut(s) 160, 881, 930, 991
BseGI GGATG 3 cut(s) 220, 891, 1156
BseLI CCNNNNNNNGG 4 cut(s) 41, 408, 484, 813
BseMI GCAATG 4 cut(s) 180, 541, 708, 741
BseNI ACTGG 5 cut(s) 50, 207, 352, 1143, 1186
BseRI GAGGAG 2 cut(s) 630, 1229
BseXI GCAGC 3 cut(s) 157, 704, 886
Bsh1236I CGCG 1 cut(s) 1055
BshFI GGCC 4 cut(s) 358, 1134, 1163, 1227
BshNI GGYRCC 2 cut(s) 46, 622
BsiHKAI GWGCWC 1 cut(s) 469
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 4 cut(s) 41, 408, 484, 813
BsmAI GTCTC 4 cut(s) 78, 513, 516, 1017
BsmBI CGTCTC 1 cut(s) 1017
BsmFI GGGAC 1 cut(s) 978
BsnI GGCC 4 cut(s) 358, 1134, 1163, 1227
Bsp119I TTCGAA 1 cut(s) 772
Bsp1286I GDGCHC 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 4 cut(s) 61, 339, 488, 1101
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 2 cut(s) 641, 777
BspANI GGCC 4 cut(s) 358, 1134, 1163, 1227
BspFNI CGCG 1 cut(s) 1055
BspLI GGNNCC 5 cut(s) 48, 490, 538, 624, 1162
BspPI GGATC 2 cut(s) 483, 496
BspT104I TTCGAA 1 cut(s) 772
BspT107I GGYRCC 2 cut(s) 46, 622
BsrDI GCAATG 4 cut(s) 180, 541, 708, 741
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 5 cut(s) 50, 207, 352, 1143, 1186
BssECI CCNNGG 4 cut(s) 160, 881, 930, 991
BssMI GATC 4 cut(s) 61, 339, 488, 1101
BssT1I CCWWGG 2 cut(s) 160, 930
Bst2UI CCWGG 1 cut(s) 992
Bst4CI ACNGT 7 cut(s) 298, 479, 668, 719, 865, 882, 917
BstAPI GCANNNNNTGC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 4 cut(s) 6, 655, 748, 1242
BstDEI CTNAG 1 cut(s) 80
BstDSI CCRYGG 2 cut(s) 160, 881
BstF5I GGATG 3 cut(s) 220, 891, 1156
BstFNI CGCG 1 cut(s) 1055
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 4 cut(s) 64, 342, 491, 1104
BstMAI GTCTC 4 cut(s) 78, 513, 516, 1017
BstMBI GATC 4 cut(s) 61, 339, 488, 1101
BstMWI GCNNNNNNNGC 8 cut(s) 125, 364, 554, 638, 743, 791, 800, 1131
BstNI CCWGG 1 cut(s) 992
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 990
BstUI CGCG 1 cut(s) 1055
BstV1I GCAGC 3 cut(s) 157, 704, 886
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 488
BstYI RGATCY 1 cut(s) 488
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 4 cut(s) 358, 1134, 1163, 1227
BtgI CCRYGG 2 cut(s) 160, 881
BtrI CACGTC 1 cut(s) 1252
BtsCI GGATG 3 cut(s) 220, 891, 1156
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 3 cut(s) 345, 841, 1150
Cac8I GCNNGC 4 cut(s) 6, 655, 748, 1242
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 5 cut(s) 392, 848, 1133, 1161, 1226
CseI GACGC 1 cut(s) 1034
Csp6I GTAC 1 cut(s) 646
CviAII CATG 7 cut(s) 68, 122, 161, 270, 533, 654, 983
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 1 cut(s) 80
DpnI GATC 4 cut(s) 63, 341, 490, 1103
DpnII GATC 4 cut(s) 61, 339, 488, 1101
EaeI YGGCCR 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 160, 930
Eco32I GATATC 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 392, 848
Eco57I CTGAAG 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 990
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 160, 930
ErhI CCWWGG 2 cut(s) 160, 930
Esp3I CGTCTC 1 cut(s) 1017
FaeI CATG 7 cut(s) 71, 125, 164, 273, 536, 657, 986
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 1 cut(s) 978
FatI CATG 7 cut(s) 67, 121, 160, 269, 532, 653, 982
FauNDI CATATG 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 171, 693, 900
FokI GGATG 3 cut(s) 227, 898, 1163
Fsp4HI GCNGC 3 cut(s) 171, 693, 900
FspBI CTAG 2 cut(s) 689, 821
FspI TGCGCA 1 cut(s) 547
GlaI GCGC 1 cut(s) 547
GluI GCNGC 3 cut(s) 171, 693, 900
GsuI CTGGAG 2 cut(s) 974, 1203
HaeIII GGCC 4 cut(s) 358, 1134, 1163, 1227
HgaI GACGC 1 cut(s) 1034
HhaI GCGC 1 cut(s) 548
Hin1II CATG 7 cut(s) 71, 125, 164, 273, 536, 657, 986
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 4 cut(s) 362, 509, 521, 1097
HphI GGTGA 2 cut(s) 919, 1184
Hpy166II GTNNAC 2 cut(s) 94, 646
Hpy188I TCNGA 7 cut(s) 464, 526, 676, 852, 1106, 1194, 1256
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 2 cut(s) 94, 646
Hpy99I CGWCG 1 cut(s) 1256
HpyAV CCTTC 3 cut(s) 521, 839, 1037
HpyCH4III ACNGT 7 cut(s) 298, 479, 668, 719, 865, 882, 917
HpyCH4IV ACGT 1 cut(s) 1251
HpyCH4V TGCA 9 cut(s) 4, 119, 173, 746, 785, 834, 878, 1178, 1244
HpyF10VI GCNNNNNNNGC 8 cut(s) 125, 364, 554, 638, 743, 791, 800, 1131
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 1251
Hsp92II CATG 7 cut(s) 71, 125, 164, 273, 536, 657, 986
HspAI GCGC 1 cut(s) 546
Kzo9I GATC 4 cut(s) 61, 339, 488, 1101
LmnI GCTCC 2 cut(s) 464, 1216
Lsp1109I GCAGC 3 cut(s) 157, 704, 886
LweI GCATC 6 cut(s) 557, 667, 694, 876, 1129, 1165
MaeI CTAG 2 cut(s) 689, 821
MaeII ACGT 1 cut(s) 1251
MaeIII GTNAC 4 cut(s) 52, 446, 839, 1049
MalI GATC 4 cut(s) 63, 341, 490, 1103
MboI GATC 4 cut(s) 61, 339, 488, 1101
MboII GAAGA 7 cut(s) 56, 128, 223, 509, 512, 673, 1274
MfeI CAATTG 2 cut(s) 786, 938
MflI RGATCY 1 cut(s) 488
MhlI GDGCHC 1 cut(s) 469
MlsI TGGCCA 1 cut(s) 358
MluCI AATT 7 cut(s) 351, 456, 786, 938, 998, 1113, 1275
MluNI TGGCCA 1 cut(s) 358
MlyI GAGTC 2 cut(s) 515, 518
MmeI TCCRAC 1 cut(s) 1234
MnlI CCTC 9 cut(s) 405, 608, 611, 955, 998, 1100, 1124, 1162, 1207
Mox20I TGGCCA 1 cut(s) 358
MscI TGGCCA 1 cut(s) 358
MseI TTAA 4 cut(s) 105, 300, 1010, 1088
Msp20I TGGCCA 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 992
MunI CAATTG 2 cut(s) 786, 938
MvaI CCWGG 1 cut(s) 992
MvnI CGCG 1 cut(s) 1055
MwoI GCNNNNNNNGC 8 cut(s) 125, 364, 554, 638, 743, 791, 800, 1131
NcoI CCATGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 419
NdeII GATC 4 cut(s) 61, 339, 488, 1101
NlaIII CATG 7 cut(s) 71, 125, 164, 273, 536, 657, 986
NlaIV GGNNCC 5 cut(s) 48, 490, 538, 624, 1162
NmeAIII GCCGAG 1 cut(s) 713
NsbI TGCGCA 1 cut(s) 547
NspI RCATGY 1 cut(s) 657
NspV TTCGAA 1 cut(s) 772
PaeI GCATGC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 475
PfeI GAWTC 2 cut(s) 362, 1097
PflFI GACNNNGTC 2 cut(s) 719, 1060
PflMI CCANNNNNTGG 1 cut(s) 408
PkrI GCNGC 3 cut(s) 172, 694, 901
PleI GAGTC 2 cut(s) 515, 517
PpsI GAGTC 2 cut(s) 515, 517
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 990
PspGI CCWGG 1 cut(s) 990
PspN4I GGNNCC 5 cut(s) 48, 490, 538, 624, 1162
PspPI GGNCC 5 cut(s) 392, 848, 1133, 1161, 1226
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 488
PsyI GACNNNGTC 2 cut(s) 719, 1060
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
SaqAI TTAA 4 cut(s) 105, 300, 1010, 1088
SatI GCNGC 3 cut(s) 171, 693, 900
Sau3AI GATC 4 cut(s) 61, 339, 488, 1101
Sau96I GGNCC 5 cut(s) 392, 848, 1133, 1161, 1226
SchI GAGTC 2 cut(s) 515, 518
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 1 cut(s) 469
SfaNI GCATC 6 cut(s) 557, 667, 694, 876, 1129, 1165
SfuI TTCGAA 1 cut(s) 772
SinI GGWCC 2 cut(s) 392, 848
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SpeI ACTAGT 1 cut(s) 820
SphI GCATGC 1 cut(s) 657
Sse9I AATT 7 cut(s) 351, 456, 786, 938, 998, 1113, 1275
SsiI CCGC 2 cut(s) 641, 777
SspI AATATT 1 cut(s) 76
SspMI CTAG 2 cut(s) 689, 821
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 2 cut(s) 160, 930
TaaI ACNGT 7 cut(s) 298, 479, 668, 719, 865, 882, 917
TaiI ACGT 1 cut(s) 1254
TaqI TCGA 5 cut(s) 342, 723, 772, 1100, 1212
TasI AATT 7 cut(s) 351, 456, 786, 938, 998, 1113, 1275
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 362, 1097
Tru1I TTAA 4 cut(s) 105, 300, 1010, 1088
Tru9I TTAA 4 cut(s) 105, 300, 1010, 1088
TscAI CASTG 3 cut(s) 352, 841, 1150
TseI GCWGC 3 cut(s) 170, 692, 899
TspDTI ATGAA 3 cut(s) 56, 138, 913
TspRI CASTG 3 cut(s) 352, 841, 1150
Tth111I GACNNNGTC 2 cut(s) 719, 1060
Van91I CCANNNNNTGG 1 cut(s) 408
VpaK11BI GGWCC 2 cut(s) 392, 848
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 3 cut(s) 351, 998, 1275
XceI RCATGY 1 cut(s) 657
XcmI CCANNNNNNNNNTGG 2 cut(s) 405, 937
XspI CTAG 2 cut(s) 689, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.