RH1AG189100

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000390
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG189100.1

Sequence Viewer

Length: 2202 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTCTACTTCTTGTTCATGTTACTCACTCAACTGCTCGGGATGCCCGGAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAAAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAATGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCAACCTTGAAAAGGATATCATCATCGGTGTGATTGACAGTGGAATTTGGCCAGAATCAGACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTATTACCGTCATTTGGGATCCTTCGAAAAAGAAGTTGAGTCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCGGTAGCAGCTGGGAACATAGTGAAAAACACGAGCATAAAAGGTTTAGGGTTTGGAAACGCAAGAGGAGGTGTGCCATCAGCACATATTGCAGTGTACAAAGTGTGCTGGAAGGATGGTTGTTCAGATGCTGACATGCTAGCGGCATTCGATGATGCAGTTGCTGATGGTGTCGACATAATCTCTATTTCCATTGGAAGCAAAGTTCCAAATGATTATTTTCGAAATGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAATGGGATACTTGTTTCAACGGCCAGTGGTAACGAAGGTCCGAAAAAGAAAACTGTGTCAAACTTTGCACCGTGGATGCTTTCTGTGGCTGCTACTACCATAAACCGTCAGTTCATCACCAAGGTTCAATTAGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATATATGGCGGAGACGCACCTAACACAACAGAAGGTTCTAACGCATCTATGTCCAGGTTCTGTTCCGAAAATAGCTTAAAAGAAGATTCGATCAGAGATAAAATTGTGCTTTGTGATAGAGGCCCTGTTGGCAATGACGATGGGTATGGGGCCATACAGGGTGATGCAACCGGTGTTATTCTGACGGGCGTAAAAATTGCCGAGGAGCTGGTTGGCCCTCTTACCCTGCCTGCATCTCACATCGGATTGGAAGAAAACAGCCACATTTACAAATACATAAGTGAAACAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAATCCAATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCCCCAGGTACTCATATTTTAGCAGCATATGATCATCAATCGGTTGGTGGTGGCAGTAGAGCTAAGTATAATTTGGAGACTGGGACATCAATGGCATGCCCCCATGCTGCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCGGCTCTCATCACTACAGCTAAACCTATGAGTACTAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCTTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTAGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATATAGTGGAAAACTATTGGAAATCATAACCAGGGACAAGAGTAGATGCTCATCAAAATCTAATAATGAAACAACCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATTAAGGATCCAGAATTCATCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCGAAATCAATGTGAATCCAAGTGTGCTATCATTTACATCTCTCGGGCAGAAGGAATCTTTTGTTGTTACAGTAAAAGGGTCAATTGAGAAATCAAATATAGTCTCTGCATCTTTGGTGTGGGATGATGGTACTTTCCAAGTCAGGAGCCCGATTGTTATCCATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

733

Amino Acids

79.19

Weight (kDa)

7.29

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 1.2e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 576 1.2e-45 Subtilase family
fn3_6 PF17766 632 - 729 3.6e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 634
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 2 cut(s) 495, 1757
AccI GTMKAC 2 cut(s) 810, 1767
AciI CCGC 3 cut(s) 779, 1107, 1700
AclWI GGATC 5 cut(s) 570, 583, 1925, 1938, 1987
AcoI YGGCCR 2 cut(s) 443, 918
AcsI RAATTY 5 cut(s) 438, 1085, 1709, 1937, 1987
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 4 cut(s) 734, 1495, 1687, 2158
AfiI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1744, 1757
AgeI ACCGGT 1 cut(s) 1268
AgsI TTSAA 5 cut(s) 404, 915, 1025, 1652, 1923
AjnI CCWGG 4 cut(s) 1151, 1489, 1756, 1844
AloI GAACNNNNNNTCC 2 cut(s) 825, 857
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 6 cut(s) 165, 600, 603, 1104, 1556, 2134
AlwI GGATC 5 cut(s) 570, 583, 1925, 1938, 1987
AlwNI CAGNNNCTG 3 cut(s) 767, 800, 1158
Ama87I CYCGRG 2 cut(s) 78, 2069
AoxI GGCC 5 cut(s) 443, 918, 1219, 1248, 1312
ApeKI GCWGC 8 cut(s) 257, 644, 986, 1484, 1508, 1592, 1604, 1643
ApoI RAATTY 5 cut(s) 438, 1085, 1709, 1937, 1987
AseI ATTAAT 2 cut(s) 192, 1398
AsiGI ACCGGT 1 cut(s) 1268
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1442
AsuC2I CCSGG 2 cut(s) 88, 1079
AsuHPI GGTGA 3 cut(s) 1006, 1271, 1629
AsuII TTCGAA 2 cut(s) 582, 859
AsuNHI GCTAGC 1 cut(s) 775
AvaI CYCGRG 2 cut(s) 78, 2069
AvaII GGWCC 3 cut(s) 479, 935, 1442
BaeGI GKGCMC 1 cut(s) 1425
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 575, 1930
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 1750, 2177
BauI CACGAG 1 cut(s) 667
BbsI GAAGAC 2 cut(s) 310, 1945
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 8 cut(s) 244, 656, 973, 1496, 1520, 1579, 1591, 1630
BccI CCATC 8 cut(s) 6, 296, 721, 746, 797, 1232, 2026, 2147
BceAI ACGGC 1 cut(s) 933
BciT130I CCWGG 4 cut(s) 1153, 1491, 1758, 1846
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 1516
BcnI CCSGG 2 cut(s) 88, 1079
BcoDI GTCTC 6 cut(s) 165, 600, 603, 1104, 1556, 2134
BfaI CTAG 3 cut(s) 776, 1695, 1743
BfmI CTRYAG 2 cut(s) 1593, 1668
BfuI GTATCC 1 cut(s) 897
BglI GCCNNNNNGGC 2 cut(s) 92, 1227
BisI GCNGC 9 cut(s) 258, 645, 780, 987, 1485, 1509, 1593, 1605, 1644
BlsI GCNGC 9 cut(s) 259, 646, 781, 988, 1486, 1510, 1594, 1606, 1645
BmcAI AGTACT 1 cut(s) 1687
Bme1390I CCNGG 6 cut(s) 88, 1079, 1153, 1491, 1758, 1846
Bme18I GGWCC 3 cut(s) 479, 935, 1442
BmeT110I CYCGRG 2 cut(s) 78, 2069
BmgT120I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1442
BmrFI CCNGG 6 cut(s) 88, 1079, 1153, 1491, 1758, 1846
BmrI ACTGGG 1 cut(s) 1575
BmtI GCTAGC 1 cut(s) 779
BmuI ACTGGG 1 cut(s) 1575
BpiI GAAGAC 2 cut(s) 310, 1945
Bpu14I TTCGAA 2 cut(s) 582, 859
BpuEI CTTGAG 2 cut(s) 389, 1454
BpuMI CCSGG 2 cut(s) 88, 1079
BsaAI YACGTR 1 cut(s) 1420
BsaBI GATNNNNATC 2 cut(s) 1864, 2183
BsaJI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1489, 1845
BsaWI WCCGGW 1 cut(s) 1268
BsaXI ACNNNNNCTCC 2 cut(s) 1409, 1439
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1744, 1757
Bse118I RCCGGY 2 cut(s) 1268, 1639
Bse1I ACTGG 4 cut(s) 137, 294, 921, 1570
Bse3DI GCAATG 3 cut(s) 267, 628, 1237
Bse8I GATNNNNATC 2 cut(s) 1864, 2183
BseBI CCWGG 4 cut(s) 1153, 1491, 1758, 1846
BseDI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1489, 1845
BseGI GGATG 5 cut(s) 88, 307, 757, 978, 2155
BseJI GATNNNNATC 2 cut(s) 1864, 2183
BseLI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1744, 1757
BseMI GCAATG 3 cut(s) 267, 628, 1237
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 4 cut(s) 137, 294, 921, 1570
BseRI GAGGAG 2 cut(s) 717, 1316
BseSI GKGCMC 1 cut(s) 1425
BseXI GCAGC 8 cut(s) 244, 656, 973, 1496, 1520, 1579, 1591, 1630
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 5 cut(s) 445, 920, 1221, 1250, 1314
BshNI GGYRCC 1 cut(s) 133
BshTI ACCGGT 1 cut(s) 1268
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 78, 2069
BsiSI CCGG 4 cut(s) 88, 1078, 1269, 1640
BslFI GGGAC 3 cut(s) 1065, 1582, 1862
BslI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1744, 1757
BsmAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1556, 2134
BsmBI CGTCTC 2 cut(s) 165, 1104
BsmFI GGGAC 3 cut(s) 1065, 1582, 1862
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 5 cut(s) 445, 920, 1221, 1250, 1314
BsoBI CYCGRG 2 cut(s) 78, 2069
Bsp119I TTCGAA 2 cut(s) 582, 859
Bsp1286I GDGCHC 4 cut(s) 556, 1425, 1750, 2177
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 3 cut(s) 779, 1107, 1700
BspANI GGCC 5 cut(s) 445, 920, 1221, 1250, 1314
BspCNI CTCAG 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 1597
BspOI GCTAGC 1 cut(s) 779
BspPI GGATC 5 cut(s) 570, 583, 1925, 1938, 1987
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 2 cut(s) 582, 859
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 3 cut(s) 267, 628, 1237
BsrFI RCCGGY 2 cut(s) 1268, 1639
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 4 cut(s) 137, 294, 921, 1570
BssAI RCCGGY 2 cut(s) 1268, 1639
BssECI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1489, 1845
BssMI GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
BssNAI GTATAC 1 cut(s) 1768
BssSI CACGAG 1 cut(s) 667
BssT1I CCWWGG 2 cut(s) 247, 1017
Bst1107I GTATAC 1 cut(s) 1768
Bst2BI CACGAG 1 cut(s) 667
Bst2UI CCWGG 4 cut(s) 1153, 1491, 1758, 1846
Bst6I CTCTTC 2 cut(s) 12, 1386
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 1420
BstBI TTCGAA 2 cut(s) 582, 859
BstC8I GCNNGC 7 cut(s) 777, 1329, 1582, 1597, 1700, 1704, 1726
BstDEI CTNAG 2 cut(s) 26, 1548
BstDSI CCRYGG 2 cut(s) 247, 968
BstEII GGTNACC 1 cut(s) 1635
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 5 cut(s) 88, 307, 757, 978, 2155
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 7 cut(s) 151, 578, 1191, 1519, 1774, 1933, 1982
BstMAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1556, 2134
BstMBI GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
BstNI CCWGG 4 cut(s) 1153, 1491, 1758, 1846
BstNSI RCATGY 2 cut(s) 775, 1584
BstPI GGTNACC 1 cut(s) 1635
BstSCI CCNGG 6 cut(s) 86, 1077, 1151, 1489, 1756, 1844
BstSFI CTRYAG 2 cut(s) 1593, 1668
BstSLI GKGCMC 1 cut(s) 1425
BstV1I GCAGC 8 cut(s) 244, 656, 973, 1496, 1520, 1579, 1591, 1630
BstV2I GAAGAC 2 cut(s) 310, 1945
BstX2I RGATCY 2 cut(s) 575, 1930
BstYI RGATCY 2 cut(s) 575, 1930
BstZ17I GTATAC 1 cut(s) 1768
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 5 cut(s) 445, 920, 1221, 1250, 1314
BtgI CCRYGG 2 cut(s) 247, 968
BtsCI GGATG 5 cut(s) 88, 307, 757, 978, 2155
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 439, 735, 928
Cac8I GCNNGC 7 cut(s) 777, 1329, 1582, 1597, 1700, 1704, 1726
CaiI CAGNNNCTG 3 cut(s) 767, 800, 1158
CfoI GCGC 1 cut(s) 635
Cfr10I RCCGGY 2 cut(s) 1268, 1639
Cfr13I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1442
CseI GACGC 2 cut(s) 328, 1121
Csp6I GTAC 4 cut(s) 733, 1494, 1686, 2157
CspAI ACCGGT 1 cut(s) 1268
CviQI GTAC 4 cut(s) 733, 1494, 1686, 2157
DdeI CTNAG 2 cut(s) 26, 1548
DpnI GATC 7 cut(s) 150, 577, 1190, 1518, 1773, 1932, 1981
DpnII GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
EaeI YGGCCR 2 cut(s) 443, 918
Eam1104I CTCTTC 2 cut(s) 12, 1386
EarI CTCTTC 2 cut(s) 12, 1386
EciI GGCGGA 1 cut(s) 1122
Eco130I CCWWGG 2 cut(s) 247, 1017
Eco24I GRGCYC 2 cut(s) 1750, 2177
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 3 cut(s) 479, 935, 1442
Eco57I CTGAAG 1 cut(s) 633
Eco88I CYCGRG 2 cut(s) 78, 2069
Eco91I GGTNACC 1 cut(s) 1635
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 1635
EcoRI GAATTC 2 cut(s) 1937, 1987
EcoRII CCWGG 4 cut(s) 1151, 1489, 1756, 1844
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 2 cut(s) 247, 1017
EcoT38I GRGCYC 2 cut(s) 1750, 2177
ErhI CCWWGG 2 cut(s) 247, 1017
Esp3I CGTCTC 2 cut(s) 165, 1104
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 3 cut(s) 1065, 1582, 1862
FauI CCCGC 1 cut(s) 1707
FauNDI CATATG 3 cut(s) 506, 1513, 1717
FbaI TGATCA 1 cut(s) 1516
FblI GTMKAC 2 cut(s) 810, 1767
Fnu4HI GCNGC 9 cut(s) 258, 645, 780, 987, 1485, 1509, 1593, 1605, 1644
FokI GGATG 5 cut(s) 95, 314, 764, 985, 2162
FriOI GRGCYC 2 cut(s) 1750, 2177
Fsp4HI GCNGC 9 cut(s) 258, 645, 780, 987, 1485, 1509, 1593, 1605, 1644
FspBI CTAG 3 cut(s) 776, 1695, 1743
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 1 cut(s) 634
GluI GCNGC 9 cut(s) 258, 645, 780, 987, 1485, 1509, 1593, 1605, 1644
GsaI CCCAGC 1 cut(s) 651
HaeIII GGCC 5 cut(s) 445, 920, 1221, 1250, 1314
HapII CCGG 4 cut(s) 88, 1078, 1269, 1640
HgaI GACGC 2 cut(s) 328, 1121
HhaI GCGC 1 cut(s) 635
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 2 cut(s) 181, 811
HindII GTYRAC 2 cut(s) 181, 811
HinfI GANTC 8 cut(s) 449, 596, 608, 1184, 1456, 2025, 2041, 2081
HpaII CCGG 4 cut(s) 88, 1078, 1269, 1640
HphI GGTGA 3 cut(s) 1006, 1271, 1629
Hpy166II GTNNAC 5 cut(s) 181, 733, 811, 1768, 1808
Hpy188I TCNGA 9 cut(s) 454, 551, 613, 763, 939, 1165, 1193, 1281, 1343
Hpy188III TCNNGA 6 cut(s) 80, 377, 1775, 1934, 2022, 2170
Hpy8I GTNNAC 5 cut(s) 181, 733, 811, 1768, 1808
Hpy99I CGWCG 1 cut(s) 1987
HpyCH4IV ACGT 3 cut(s) 174, 1419, 1611
HpyF3I CTNAG 2 cut(s) 26, 1548
HpySE526I ACGT 3 cut(s) 174, 1419, 1611
HspAI GCGC 1 cut(s) 633
Ksp22I TGATCA 1 cut(s) 1516
Kzo9I GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 551, 1303, 1417, 1721, 1753, 2020, 2172
Lsp1109I GCAGC 8 cut(s) 244, 656, 973, 1496, 1520, 1579, 1591, 1630
MaeI CTAG 3 cut(s) 776, 1695, 1743
MaeII ACGT 3 cut(s) 174, 1419, 1611
MaeIII GTNAC 9 cut(s) 61, 139, 533, 926, 1031, 1635, 1966, 2092, 2191
MalI GATC 7 cut(s) 150, 577, 1190, 1518, 1773, 1932, 1981
MboI GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
MboII GAAGA 7 cut(s) 143, 215, 310, 1193, 1361, 1403, 1945
MfeI CAATTG 2 cut(s) 873, 2109
MflI RGATCY 2 cut(s) 575, 1930
MhlI GDGCHC 4 cut(s) 556, 1425, 1750, 2177
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 3 cut(s) 602, 605, 2019
MmeI TCCRAC 1 cut(s) 1957
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 7 cut(s) 192, 387, 1097, 1175, 1398, 1926, 2200
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 4 cut(s) 88, 1078, 1269, 1640
MspR9I CCNGG 6 cut(s) 88, 1079, 1153, 1491, 1758, 1846
MunI CAATTG 2 cut(s) 873, 2109
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 4 cut(s) 1153, 1491, 1758, 1846
NciI CCSGG 2 cut(s) 88, 1079
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 3 cut(s) 506, 1513, 1717
NdeII GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
NheI GCTAGC 1 cut(s) 775
NmeAIII GCCGAG 1 cut(s) 1324
NmuCI GTSAC 2 cut(s) 1635, 1966
NsbI TGCGCA 1 cut(s) 634
NspI RCATGY 2 cut(s) 775, 1584
NspV TTCGAA 2 cut(s) 582, 859
PaeI GCATGC 1 cut(s) 1584
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 562
PctI GAATGC 1 cut(s) 782
PfeI GAWTC 5 cut(s) 449, 1184, 1456, 2041, 2081
PflMI CCANNNNNTGG 2 cut(s) 495, 1757
PinAI ACCGGT 1 cut(s) 1268
PkrI GCNGC 9 cut(s) 259, 646, 781, 988, 1486, 1510, 1594, 1606, 1645
PleI GAGTC 3 cut(s) 602, 604, 2019
PpsI GAGTC 3 cut(s) 602, 604, 2019
Ppu21I YACGTR 1 cut(s) 1420
PshBI ATTAAT 2 cut(s) 192, 1398
Psp6I CCWGG 4 cut(s) 1151, 1489, 1756, 1844
PspEI GGTNACC 1 cut(s) 1635
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 4 cut(s) 1151, 1489, 1756, 1844
PspPI GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1442
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 3 cut(s) 767, 800, 1158
PsuI RGATCY 2 cut(s) 575, 1930
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 4 cut(s) 734, 1495, 1687, 2158
RsaNI GTAC 4 cut(s) 733, 1494, 1686, 2157
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 7 cut(s) 192, 387, 1097, 1175, 1398, 1926, 2200
SatI GCNGC 9 cut(s) 258, 645, 780, 987, 1485, 1509, 1593, 1605, 1644
Sau3AI GATC 7 cut(s) 148, 575, 1188, 1516, 1771, 1930, 1979
Sau96I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1442
ScaI AGTACT 1 cut(s) 1687
SchI GAGTC 3 cut(s) 602, 605, 2019
ScrFI CCNGG 6 cut(s) 88, 1079, 1153, 1491, 1758, 1846
SduI GDGCHC 4 cut(s) 556, 1425, 1750, 2177
SfcI CTRYAG 2 cut(s) 1593, 1668
SfuI TTCGAA 2 cut(s) 582, 859
SinI GGWCC 3 cut(s) 479, 935, 1442
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 368, 1469
SmoI CTYRAG 2 cut(s) 368, 1469
SphI GCATGC 1 cut(s) 1584
SsiI CCGC 3 cut(s) 779, 1107, 1700
SspI AATATT 1 cut(s) 163
SspMI CTAG 3 cut(s) 776, 1695, 1743
StyD4I CCNGG 6 cut(s) 86, 1077, 1151, 1489, 1756, 1844
StyI CCWWGG 2 cut(s) 247, 1017
TaiI ACGT 3 cut(s) 177, 1422, 1614
TaqI TCGA 8 cut(s) 10, 582, 786, 810, 859, 1187, 1985, 2028
TatI WGTACW 2 cut(s) 732, 1685
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 5 cut(s) 449, 1184, 1456, 2041, 2081
Tru1I TTAA 7 cut(s) 192, 387, 1097, 1175, 1398, 1926, 2200
Tru9I TTAA 7 cut(s) 192, 387, 1097, 1175, 1398, 1926, 2200
TscAI CASTG 3 cut(s) 439, 735, 928
TseFI GTSAC 2 cut(s) 1635, 1966
TseI GCWGC 8 cut(s) 257, 644, 986, 1484, 1508, 1592, 1604, 1643
Tsp45I GTSAC 2 cut(s) 1635, 1966
TspDTI ATGAA 8 cut(s) 47, 143, 225, 1000, 1469, 1794, 1896, 1930
TspRI CASTG 3 cut(s) 439, 735, 928
Van91I CCANNNNNTGG 2 cut(s) 495, 1757
VpaK11BI GGWCC 3 cut(s) 479, 935, 1442
VspI ATTAAT 2 cut(s) 192, 1398
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 5 cut(s) 438, 1085, 1709, 1937, 1987
XceI RCATGY 2 cut(s) 775, 1584
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmiI GTMKAC 2 cut(s) 810, 1767
XspI CTAG 3 cut(s) 776, 1695, 1743
ZrmI AGTACT 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.