Rh1BG319000

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000390
Physical Location & Seq
Reverse (-)
45292296 .. 45308520
16225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG319000.1

Sequence Viewer

Length: 2253 bp
ATGGCTCTTCGTTGGTTTCTCTTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTTGTCGATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTTGACAATAGCAACATTGATATTGCACATGAATATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAGAGGAGCGTCAAAACAACAAAGTCATGGAACTTTCTTGGGTTTCCAGAAACAGTGAAAAGAGGCGCCCTTGAAAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCAAAATATTATCGTGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACTGCATCAACAGCAGCTGGGAACACAGTGATCAAGGCGAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATGTTCTAGCCGCATTCGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCGTCGGGGGCACTATTCCACTTGATTATTTGAATGATCCATTTGCAATTGGGTCATTTCACGCTACTGGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGCTCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGGCCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGTGCCCAATACAAAAGCAGGTGCTGACGGGAGTATCTCTAGGTTTTGTGTAAGAGATGCCTTAGAAGAAGATTTGATCAAAGGTAAAATTGTGTTGTGTGATGGCCCTATGGATGGGGCTGGGGATGGGGCCATATTGGGTGGTGCAGCCGGAATTGTTCTGACGGGAAAAAAAGTCGACGATGAACAACTTGGCCCTTATCCCGTGCCTGCAACTTTCGTCGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAACTCAACAAGGAACGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATATGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAGATCCCAATATTCTCAAGGTGACTTGATTCATAATGAAATATTAATTCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGATGGTAGAGCTGGATACTATATAGATACCGGGACATCAATGGCATGCCCCCATGCTACGGCCACAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCAGCGGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCCATGAGTGCCAAAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAAATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAGAGACAAGACTAGTTGCTCATCAAAATCTAATAATGGAACAGCTAATGACATGAACTATCATTCTTTTGCACTTTCCATCAAGGATCCGAAATTTGTCATTGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAGTCCACATACAGAGCTAAAGTAGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATCTAGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATTTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

750

Amino Acids

79.98

Weight (kDa)

7.15

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 113 1.5e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 593 2.3e-38 Subtilase family
fn3_6 PF17766 649 - 746 3.7e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1127
Acc36I ACCTGC 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 4 cut(s) 136, 395, 709, 1798
AccB7I CCANNNNNTGG 3 cut(s) 495, 1583, 1808
AccI GTMKAC 2 cut(s) 816, 1296
AccII CGCG 1 cut(s) 721
AciI CCGC 5 cut(s) 786, 927, 1009, 1541, 1691
AclWI GGATC 5 cut(s) 863, 1479, 1976, 1989, 2038
AcoI YGGCCR 3 cut(s) 443, 924, 1647
AcsI RAATTY 5 cut(s) 438, 1091, 1760, 1846, 1988
AcyI GRCGYC 2 cut(s) 396, 1799
AfaI GTAC 3 cut(s) 138, 734, 1820
AgsI TTSAA 5 cut(s) 247, 404, 583, 865, 1031
AhlI ACTAGT 1 cut(s) 1907
AjiI CACGTC 1 cut(s) 1120
AjnI CCWGG 2 cut(s) 1546, 1807
Alw26I GTCTC 5 cut(s) 600, 603, 1110, 1893, 2185
AlwI GGATC 5 cut(s) 863, 1479, 1976, 1989, 2038
AlwNI CAGNNNCTG 3 cut(s) 647, 1142, 1694
Ama87I CYCGRG 1 cut(s) 2120
ApaI GGGCCC 1 cut(s) 1472
ApeKI GCWGC 9 cut(s) 77, 257, 644, 750, 992, 1265, 1565, 1655, 1694
ApoI RAATTY 5 cut(s) 438, 1091, 1760, 1846, 1988
ArsI GACNNNNNNTTYG 2 cut(s) 1745, 1777
AseI ATTAAT 1 cut(s) 1526
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 3 cut(s) 398, 721, 1801
AspS9I GGNCC 8 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 2156
AsuC2I CCSGG 3 cut(s) 945, 1085, 1618
AsuHPI GGTGA 4 cut(s) 1012, 1514, 1598, 1667
AvaI CYCGRG 1 cut(s) 2120
AvaII GGWCC 2 cut(s) 479, 941
BaeGI GKGCMC 4 cut(s) 845, 1125, 1452, 1472
BaeI ACNNNNGTAYC 4 cut(s) 1135, 1168, 1858, 1891
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 1 cut(s) 1981
BanI GGYRCC 4 cut(s) 136, 395, 709, 1798
BanII GRGCYC 3 cut(s) 950, 1472, 2228
BbsI GAAGAC 2 cut(s) 310, 1996
BbvI GCAGC 9 cut(s) 64, 244, 656, 762, 979, 1277, 1577, 1642, 1681
BccI CCATC 8 cut(s) 296, 721, 1214, 1226, 1238, 1583, 1982, 2198
BceAI ACGGC 1 cut(s) 1662
BciT130I CCWGG 2 cut(s) 1548, 1809
BciVI GTATCC 2 cut(s) 1595, 1859
BclI TGATCA 2 cut(s) 660, 1194
BcnI CCSGG 3 cut(s) 945, 1085, 1618
BcoDI GTCTC 5 cut(s) 600, 603, 1110, 1893, 2185
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 6 cut(s) 782, 1158, 1746, 1794, 1908, 2177
BfoI RGCGCY 2 cut(s) 399, 1802
BfuAI ACCTGC 1 cut(s) 1127
BfuI GTATCC 2 cut(s) 1595, 1859
BglI GCCNNNNNGGC 1 cut(s) 95
Bme1390I CCNGG 5 cut(s) 945, 1085, 1548, 1618, 1809
Bme18I GGWCC 2 cut(s) 479, 941
BmeT110I CYCGRG 1 cut(s) 2120
BmgBI CACGTC 1 cut(s) 1120
BmgT120I GGNCC 8 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 2156
BmrFI CCNGG 5 cut(s) 945, 1085, 1548, 1618, 1809
BmsI GCATC 6 cut(s) 76, 457, 644, 760, 787, 1165
BpiI GAAGAC 2 cut(s) 310, 1996
BpmI CTGGAG 1 cut(s) 1530
Bpu10I CCTNAGC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 1482
BpuMI CCSGG 3 cut(s) 945, 1085, 1618
BsaHI GRCGYC 2 cut(s) 396, 1799
BsaJI CCNNGG 4 cut(s) 921, 974, 1023, 1084
Bse1I ACTGG 1 cut(s) 904
Bse3DI GCAATG 3 cut(s) 267, 523, 801
BseBI CCWGG 2 cut(s) 1548, 1809
BseDI CCNNGG 4 cut(s) 921, 974, 1023, 1084
BseGI GGATG 5 cut(s) 91, 307, 1237, 1249, 2206
BseMI GCAATG 3 cut(s) 267, 523, 801
BseMII CTCAG 3 cut(s) 40, 87, 95
BseNI ACTGG 1 cut(s) 904
BseRI GAGGAG 2 cut(s) 349, 717
BseSI GKGCMC 4 cut(s) 845, 1125, 1452, 1472
BseXI GCAGC 9 cut(s) 64, 244, 656, 762, 979, 1277, 1577, 1642, 1681
BseYI CCCAGC 2 cut(s) 647, 1238
BsgI GTGCAG 1 cut(s) 1284
Bsh1236I CGCG 1 cut(s) 721
BshNI GGYRCC 4 cut(s) 136, 395, 709, 1798
BsiHKCI CYCGRG 1 cut(s) 2120
BsiSI CCGG 4 cut(s) 944, 1084, 1269, 1617
BslFI GGGAC 4 cut(s) 465, 1071, 1633, 2088
BsmAI GTCTC 5 cut(s) 600, 603, 1110, 1893, 2185
BsmBI CGTCTC 1 cut(s) 1110
BsmFI GGGAC 4 cut(s) 465, 1071, 1633, 2088
BsmI GAATGC 1 cut(s) 788
BsoBI CYCGRG 1 cut(s) 2120
Bsp120I GGGCCC 1 cut(s) 1468
Bsp1286I GDGCHC 6 cut(s) 845, 950, 1125, 1452, 1472, 2228
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
Bsp19I CCATGG 2 cut(s) 921, 974
BspACI CCGC 5 cut(s) 786, 927, 1009, 1541, 1691
BspCNI CTCAG 3 cut(s) 39, 86, 94
BspFNI CGCG 1 cut(s) 721
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 5 cut(s) 863, 1479, 1976, 1989, 2038
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 4 cut(s) 136, 395, 709, 1798
BsrDI GCAATG 3 cut(s) 267, 523, 801
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 1 cut(s) 904
BssECI CCNNGG 4 cut(s) 921, 974, 1023, 1084
BssMI GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
BssNI GRCGYC 2 cut(s) 396, 1799
BssT1I CCWWGG 3 cut(s) 921, 974, 1023
Bst2UI CCWGG 2 cut(s) 1548, 1809
Bst4CI ACNGT 9 cut(s) 385, 434, 617, 658, 812, 958, 1043, 1871, 2017
Bst6I CTCTTC 2 cut(s) 12, 1413
BstACI GRCGYC 2 cut(s) 396, 1799
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 5 cut(s) 670, 1329, 1633, 1777, 1887
BstDEI CTNAG 4 cut(s) 26, 73, 81, 1180
BstDSI CCRYGG 2 cut(s) 921, 974
BstF5I GGATG 5 cut(s) 91, 307, 1237, 1249, 2206
BstFNI CGCG 1 cut(s) 721
BstH2I RGCGCY 2 cut(s) 399, 1802
BstHHI GCGC 3 cut(s) 398, 721, 1801
BstKTI GATC 9 cut(s) 154, 663, 871, 1197, 1482, 1487, 1825, 1984, 2033
BstMAI GTCTC 5 cut(s) 600, 603, 1110, 1893, 2185
BstMBI GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
BstNI CCWGG 2 cut(s) 1548, 1809
BstNSI RCATGY 2 cut(s) 744, 1635
BstSCI CCNGG 5 cut(s) 943, 1083, 1546, 1616, 1807
BstSLI GKGCMC 4 cut(s) 845, 1125, 1452, 1472
BstUI CGCG 1 cut(s) 721
BstV1I GCAGC 9 cut(s) 64, 244, 656, 762, 979, 1277, 1577, 1642, 1681
BstV2I GAAGAC 2 cut(s) 310, 1996
BstX2I RGATCY 2 cut(s) 1484, 1981
BstYI RGATCY 2 cut(s) 1484, 1981
BsuI GTATCC 2 cut(s) 1595, 1859
BtgI CCRYGG 2 cut(s) 921, 974
BtrI CACGTC 1 cut(s) 1120
BtsCI GGATG 5 cut(s) 91, 307, 1237, 1249, 2206
BtsI GCAGTG 2 cut(s) 630, 760
BtsIMutI CAGTG 6 cut(s) 390, 439, 630, 663, 760, 1876
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 5 cut(s) 670, 1329, 1633, 1777, 1887
CaiI CAGNNNCTG 3 cut(s) 647, 1142, 1694
CfoI GCGC 3 cut(s) 398, 721, 1801
Cfr13I GGNCC 8 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 2156
CseI GACGC 3 cut(s) 328, 1763, 2175
Csp6I GTAC 3 cut(s) 137, 733, 1819
CviQI GTAC 3 cut(s) 137, 733, 1819
DdeI CTNAG 4 cut(s) 26, 73, 81, 1180
DinI GGCGCC 2 cut(s) 397, 1800
DpnI GATC 9 cut(s) 153, 662, 870, 1196, 1481, 1486, 1824, 1983, 2032
DpnII GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
EaeI YGGCCR 3 cut(s) 443, 924, 1647
Eam1104I CTCTTC 2 cut(s) 12, 1413
EarI CTCTTC 2 cut(s) 12, 1413
Eco130I CCWWGG 3 cut(s) 921, 974, 1023
Eco24I GRGCYC 3 cut(s) 950, 1472, 2228
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 479, 941
Eco88I CYCGRG 1 cut(s) 2120
EcoO109I RGGNCCY 1 cut(s) 1468
EcoRII CCWGG 2 cut(s) 1546, 1807
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 921, 974, 1023
EcoT38I GRGCYC 3 cut(s) 950, 1472, 2228
EgeI GGCGCC 2 cut(s) 397, 1800
EheI GGCGCC 2 cut(s) 397, 1800
ErhI CCWWGG 3 cut(s) 921, 974, 1023
Esp3I CGTCTC 1 cut(s) 1110
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 4 cut(s) 465, 1071, 1633, 2088
FauNDI CATATG 2 cut(s) 1444, 1660
FbaI TGATCA 2 cut(s) 660, 1194
FblI GTMKAC 2 cut(s) 816, 1296
FokI GGATG 5 cut(s) 98, 314, 1244, 1256, 2213
FriOI GRGCYC 3 cut(s) 950, 1472, 2228
FspBI CTAG 6 cut(s) 782, 1158, 1746, 1794, 1908, 2177
GlaI GCGC 3 cut(s) 397, 720, 1800
GsaI CCCAGC 2 cut(s) 651, 1242
GsuI CTGGAG 1 cut(s) 1530
HaeII RGCGCY 2 cut(s) 399, 1802
HapII CCGG 4 cut(s) 944, 1084, 1269, 1617
HgaI GACGC 3 cut(s) 328, 1763, 2175
HhaI GCGC 3 cut(s) 398, 721, 1801
Hin1I GRCGYC 2 cut(s) 396, 1799
Hin6I GCGC 3 cut(s) 396, 719, 1799
HinP1I GCGC 3 cut(s) 396, 719, 1799
HincII GTYRAC 2 cut(s) 184, 1297
HindII GTYRAC 2 cut(s) 184, 1297
HinfI GANTC 6 cut(s) 449, 596, 608, 1510, 2076, 2092
HpaII CCGG 4 cut(s) 944, 1084, 1269, 1617
HphI GGTGA 4 cut(s) 1012, 1514, 1598, 1667
Hpy166II GTNNAC 9 cut(s) 184, 733, 817, 1297, 1819, 1859, 1877, 2043, 2112
Hpy188I TCNGA 6 cut(s) 454, 551, 1281, 1479, 1484, 1986
Hpy188III TCNNGA 6 cut(s) 83, 377, 690, 1532, 1826, 2221
Hpy8I GTNNAC 9 cut(s) 184, 733, 817, 1297, 1819, 1859, 1877, 2043, 2112
Hpy99I CGWCG 3 cut(s) 839, 1301, 1343
HpyAV CCTTC 2 cut(s) 932, 1800
HpyCH4III ACNGT 9 cut(s) 385, 434, 617, 658, 812, 958, 1043, 1871, 2017
HpyCH4IV ACGT 2 cut(s) 177, 1119
HpyF3I CTNAG 4 cut(s) 26, 73, 81, 1180
HpySE526I ACGT 2 cut(s) 177, 1119
Hsp92I GRCGYC 2 cut(s) 396, 1799
HspAI GCGC 3 cut(s) 396, 719, 1799
KasI GGCGCC 2 cut(s) 395, 1798
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 2 cut(s) 660, 1194
Kzo9I GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 336, 551, 1444, 1549, 1772, 2071, 2223
Lsp1109I GCAGC 9 cut(s) 64, 244, 656, 762, 979, 1277, 1577, 1642, 1681
LweI GCATC 6 cut(s) 76, 457, 644, 760, 787, 1165
MaeI CTAG 6 cut(s) 782, 1158, 1746, 1794, 1908, 2177
MaeII ACGT 2 cut(s) 177, 1119
MaeIII GTNAC 9 cut(s) 64, 533, 764, 932, 958, 1037, 1502, 2017, 2143
MalI GATC 9 cut(s) 153, 662, 870, 1196, 1481, 1486, 1824, 1983, 2032
MboI GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
MboII GAAGA 9 cut(s) 146, 215, 310, 599, 1196, 1199, 1361, 1430, 1996
MfeI CAATTG 2 cut(s) 879, 1031
MflI RGATCY 2 cut(s) 1484, 1981
MhlI GDGCHC 6 cut(s) 845, 950, 1125, 1452, 1472, 2228
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
Mly113I GGCGCC 2 cut(s) 396, 1799
MlyI GAGTC 4 cut(s) 458, 602, 605, 2070
MmeI TCCRAC 3 cut(s) 432, 1326, 2008
MnlI CCTC 9 cut(s) 41, 327, 386, 695, 698, 1048, 1091, 1458, 1462
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 4 cut(s) 675, 1103, 1526, 2251
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 5 cut(s) 77, 647, 929, 1655, 1691
MspI CCGG 4 cut(s) 944, 1084, 1269, 1617
MspR9I CCNGG 5 cut(s) 945, 1085, 1548, 1618, 1809
MunI CAATTG 2 cut(s) 879, 1031
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 2 cut(s) 1548, 1809
MvnI CGCG 1 cut(s) 721
NarI GGCGCC 2 cut(s) 396, 1799
NciI CCSGG 3 cut(s) 945, 1085, 1618
NcoI CCATGG 2 cut(s) 921, 974
NdeI CATATG 2 cut(s) 1444, 1660
NdeII GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
NmuCI GTSAC 5 cut(s) 764, 958, 1502, 2017, 2143
NspI RCATGY 2 cut(s) 744, 1635
PaeI GCATGC 1 cut(s) 1635
PaqCI CACCTGC 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 2 cut(s) 1510, 2092
PflMI CCANNNNNTGG 3 cut(s) 495, 1583, 1808
PfoI TCCNGGA 1 cut(s) 1546
PleI GAGTC 4 cut(s) 457, 602, 604, 2070
PluTI GGCGCC 2 cut(s) 399, 1802
PpsI GAGTC 4 cut(s) 457, 602, 604, 2070
PshBI ATTAAT 1 cut(s) 1526
Psp6I CCWGG 2 cut(s) 1546, 1807
PspFI CCCAGC 2 cut(s) 647, 1238
PspGI CCWGG 2 cut(s) 1546, 1807
PspOMI GGGCCC 1 cut(s) 1468
PspPI GGNCC 8 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 2156
PstNI CAGNNNCTG 3 cut(s) 647, 1142, 1694
PsuI RGATCY 2 cut(s) 1484, 1981
PvuII CAGCTG 3 cut(s) 77, 647, 1655
RsaI GTAC 3 cut(s) 138, 734, 1820
RsaNI GTAC 3 cut(s) 137, 733, 1819
SalI GTCGAC 1 cut(s) 1295
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 4 cut(s) 675, 1103, 1526, 2251
Sau3AI GATC 9 cut(s) 151, 660, 868, 1194, 1479, 1484, 1822, 1981, 2030
Sau96I GGNCC 8 cut(s) 479, 941, 1223, 1248, 1313, 1468, 1469, 2156
SchI GAGTC 4 cut(s) 458, 602, 605, 2070
ScrFI CCNGG 5 cut(s) 945, 1085, 1548, 1618, 1809
SduI GDGCHC 6 cut(s) 845, 950, 1125, 1452, 1472, 2228
SfaNI GCATC 6 cut(s) 76, 457, 644, 760, 787, 1165
SfoI GGCGCC 2 cut(s) 397, 1800
SinI GGWCC 2 cut(s) 479, 941
SmlI CTYRAG 1 cut(s) 1497
SmoI CTYRAG 1 cut(s) 1497
SpeI ACTAGT 1 cut(s) 1907
SphI GCATGC 1 cut(s) 1635
SsiI CCGC 5 cut(s) 786, 927, 1009, 1541, 1691
SspDI GGCGCC 2 cut(s) 395, 1798
SspI AATATT 3 cut(s) 560, 1493, 1524
SspMI CTAG 6 cut(s) 782, 1158, 1746, 1794, 1908, 2177
StyD4I CCNGG 5 cut(s) 943, 1083, 1546, 1616, 1807
StyI CCWWGG 3 cut(s) 921, 974, 1023
TaaI ACNGT 9 cut(s) 385, 434, 617, 658, 812, 958, 1043, 1871, 2017
TaiI ACGT 2 cut(s) 180, 1122
TaqI TCGA 4 cut(s) 60, 792, 1296, 1463
TatI WGTACW 1 cut(s) 732
TauI GCSGC 4 cut(s) 788, 929, 1544, 1694
TfiI GAWTC 2 cut(s) 1510, 2092
Tru1I TTAA 4 cut(s) 675, 1103, 1526, 2251
Tru9I TTAA 4 cut(s) 675, 1103, 1526, 2251
TscAI CASTG 6 cut(s) 390, 439, 637, 663, 760, 1876
TseFI GTSAC 5 cut(s) 764, 958, 1502, 2017, 2143
TseI GCWGC 9 cut(s) 77, 257, 644, 750, 992, 1265, 1565, 1655, 1694
Tsp45I GTSAC 5 cut(s) 764, 958, 1502, 2017, 2143
TspDTI ATGAA 8 cut(s) 146, 225, 1006, 1317, 1502, 1533, 1845, 1964
TspGWI ACGGA 2 cut(s) 823, 1785
TspRI CASTG 6 cut(s) 390, 439, 637, 663, 760, 1876
Van91I CCANNNNNTGG 3 cut(s) 495, 1583, 1808
VpaK11BI GGWCC 2 cut(s) 479, 941
VspI ATTAAT 1 cut(s) 1526
XapI RAATTY 5 cut(s) 438, 1091, 1760, 1846, 1988
XceI RCATGY 2 cut(s) 744, 1635
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1030
XmiI GTMKAC 2 cut(s) 816, 1296
XspI CTAG 6 cut(s) 782, 1158, 1746, 1794, 1908, 2177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.