Rroxscaffold_4G00287470

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Reverse (-)
8473325 .. 8477196
3872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287470.1

Sequence Viewer

Length: 1371 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAATATTCTACTGCTTGTCGATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGACTTATGTTGTGTATATGGGCAACAAGCCAAAGAACGGGCCCATATTACCTGATCTTCATGTGAATATGCTACAGGACATAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAAGGAACGCTAAAACAACAAAGTCATGGAACTTTCTCGGATTTCCAGAAACAGTAAAGAGAAGCGCCCTTGAAAGCGATATCATTGTAGGTGTGATGGATAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCCATGGAAATCTTACTTGTAACAATAAGATCATCGGAGCACAGTATTACCGTGCTTCTCGATCCTTCAAAGAAGATGTTGAGTCTCCACGAGACTCAAGCGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGGGGTACCATTAGCACGTATTGCTGTGTACAAAATATGCTGGGGCAGCAGCATCAGTTGTGAAGATGCTGACATTCTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCTGTTGGAGGCCTTATTCCATCTGATTATTTGAATGATCCATTTGCAATTGGGTCATTTCACGCTACCAAAAATGGGATATTAGTTTCTATGGCCGCTGGTAACGAAGGTCCGAGAGCAAAAACTGTAACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAGTTCATCACCAAGGTTCAATTGGGTAACGGTAAGCTCTATGAGGGACTCTTACCAAACGTATATGACCTCCGGGCTGAATTTTATCCTTTAATTTATGGTGGAGACGCACCCAATAAAAAAGCAGGTGTTGATGGAACTATCTCCAGATTTTGTGTAAGAGATGCCTTACAAGAAGATTTGATTAAAGGTAAAATTGTGCTTTGTGATGGCCCTATCGATGGGGCTGGGGTTGGGGCCATATTGGGTGGTGCAGCTGGAATTGTTCTGACAGGAAAAAAGTTGATGATGAAGCTCTTGGCCCTTATCCCGTGCCTGCAACTTACGTCGGATTGGAAGAAAACAGCGACATTTACAAATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

456

Amino Acids

48.85

Weight (kDa)

8.01

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 110 1.7e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 334 4.2e-21 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1121
Acc36I ACCTGC 1 cut(s) 1121
Acc65I GGTACC 1 cut(s) 703
AccB1I GGYRCC 1 cut(s) 703
AccB7I CCANNNNNTGG 1 cut(s) 489
AccI GTMKAC 1 cut(s) 810
AciI CCGC 5 cut(s) 609, 627, 779, 921, 1003
AclWI GGATC 2 cut(s) 564, 857
AcoI YGGCCR 2 cut(s) 437, 918
AcsI RAATTY 2 cut(s) 432, 1085
AfaI GTAC 2 cut(s) 705, 728
AfiI CCNNNNNNNGG 4 cut(s) 128, 489, 900, 1226
AgsI TTSAA 5 cut(s) 241, 398, 577, 859, 1025
AluBI AGCT 7 cut(s) 74, 225, 453, 641, 1042, 1262, 1300
AluI AGCT 7 cut(s) 74, 225, 453, 641, 1042, 1262, 1300
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 3 cut(s) 594, 597, 1104
AlwI GGATC 2 cut(s) 564, 857
AlwNI CAGNNNCTG 1 cut(s) 641
AoxI GGCC 8 cut(s) 131, 437, 508, 835, 918, 1216, 1242, 1305
ApaI GGGCCC 1 cut(s) 135
ApeKI GCWGC 7 cut(s) 74, 251, 638, 744, 747, 986, 1259
ApoI RAATTY 2 cut(s) 432, 1085
Asp718I GGTACC 1 cut(s) 703
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 7 cut(s) 131, 132, 473, 935, 1217, 1242, 1306
AsuC2I CCSGG 1 cut(s) 1079
AsuHPI GGTGA 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 473, 935
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 7 cut(s) 61, 238, 650, 756, 759, 973, 1271
BccI CCATC 7 cut(s) 6, 290, 415, 853, 1133, 1208, 1220
BcnI CCSGG 1 cut(s) 1079
BcoDI GTCTC 3 cut(s) 594, 597, 1104
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 1121
BglII AGATCT 1 cut(s) 208
BisI GCNGC 9 cut(s) 75, 252, 639, 745, 748, 780, 921, 987, 1260
BlsI GCNGC 9 cut(s) 76, 253, 640, 746, 749, 781, 922, 988, 1261
Bme1390I CCNGG 1 cut(s) 1079
Bme18I GGWCC 2 cut(s) 473, 935
BmgT120I GGNCC 7 cut(s) 131, 132, 473, 935, 1217, 1242, 1306
BmiI GGNNCC 3 cut(s) 133, 705, 1243
BmrFI CCNGG 1 cut(s) 1079
BmsI GCATC 7 cut(s) 73, 451, 638, 754, 759, 781, 1159
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 1135
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 589
BpuMI CCSGG 1 cut(s) 1079
Bsa29I ATCGAT 1 cut(s) 1224
BsaAI YACGTR 1 cut(s) 716
BsaJI CCNNGG 3 cut(s) 511, 968, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 489, 900, 1226
Bse1I ACTGG 1 cut(s) 1006
Bse3DI GCAATG 2 cut(s) 261, 795
BseCI ATCGAT 1 cut(s) 1224
BseDI CCNNGG 3 cut(s) 511, 968, 1017
BseGI GGATG 2 cut(s) 88, 301
BseLI CCNNNNNNNGG 4 cut(s) 128, 489, 900, 1226
BseMI GCAATG 2 cut(s) 261, 795
BseMII CTCAG 2 cut(s) 84, 92
BseNI ACTGG 1 cut(s) 1006
BseRI GAGGAG 1 cut(s) 711
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 7 cut(s) 61, 238, 650, 756, 759, 973, 1271
BseYI CCCAGC 3 cut(s) 641, 738, 1232
BsgI GTGCAG 1 cut(s) 1278
BshFI GGCC 8 cut(s) 133, 439, 510, 837, 920, 1218, 1244, 1307
BshNI GGYRCC 1 cut(s) 703
BshVI ATCGAT 1 cut(s) 1224
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 1 cut(s) 1078
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 4 cut(s) 128, 489, 900, 1226
BsmAI GTCTC 3 cut(s) 594, 597, 1104
BsmBI CGTCTC 1 cut(s) 1104
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 8 cut(s) 133, 439, 510, 837, 920, 1218, 1244, 1307
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 135, 550
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 5 cut(s) 145, 208, 537, 569, 862
Bsp19I CCATGG 2 cut(s) 511, 968
BspACI CCGC 5 cut(s) 609, 627, 779, 921, 1003
BspANI GGCC 8 cut(s) 133, 439, 510, 837, 920, 1218, 1244, 1307
BspCNI CTCAG 2 cut(s) 83, 91
BspDI ATCGAT 1 cut(s) 1224
BspLI GGNNCC 3 cut(s) 133, 705, 1243
BspMI ACCTGC 1 cut(s) 1121
BspPI GGATC 2 cut(s) 564, 857
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 703
BsrDI GCAATG 2 cut(s) 261, 795
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 1 cut(s) 1006
BssECI CCNNGG 3 cut(s) 511, 968, 1017
BssMI GATC 5 cut(s) 145, 208, 537, 569, 862
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 3 cut(s) 511, 968, 1017
Bst2BI CACGAG 1 cut(s) 597
Bst4CI ACNGT 8 cut(s) 182, 379, 552, 560, 652, 806, 952, 1037
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 726
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 2 cut(s) 664, 1322
BstDEI CTNAG 2 cut(s) 70, 78
BstDSI CCRYGG 2 cut(s) 511, 968
BstF5I GGATG 2 cut(s) 88, 301
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 5 cut(s) 148, 211, 540, 572, 865
BstMAI GTCTC 3 cut(s) 594, 597, 1104
BstMBI GATC 5 cut(s) 145, 208, 537, 569, 862
BstMWI GCNNNNNNNGC 6 cut(s) 83, 265, 635, 663, 719, 744
BstSCI CCNGG 1 cut(s) 1077
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 7 cut(s) 61, 238, 650, 756, 759, 973, 1271
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 1224
BsuRI GGCC 8 cut(s) 133, 439, 510, 837, 920, 1218, 1244, 1307
BsuTUI ATCGAT 1 cut(s) 1224
BtgI CCRYGG 2 cut(s) 511, 968
BtsCI GGATG 2 cut(s) 88, 301
BtsIMutI CAGTG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 1121
Cac8I GCNNGC 2 cut(s) 664, 1322
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 7 cut(s) 131, 132, 473, 935, 1217, 1242, 1306
ClaI ATCGAT 1 cut(s) 1224
CseI GACGC 1 cut(s) 1121
Csp6I GTAC 2 cut(s) 704, 727
CviAII CATG 6 cut(s) 152, 203, 351, 512, 614, 969
CviQI GTAC 2 cut(s) 704, 727
DdeI CTNAG 2 cut(s) 70, 78
DpnI GATC 5 cut(s) 147, 210, 539, 571, 864
DpnII GATC 5 cut(s) 145, 208, 537, 569, 862
EaeI YGGCCR 2 cut(s) 437, 918
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 511, 968, 1017
Eco147I AGGCCT 1 cut(s) 837
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 473, 935
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 3 cut(s) 511, 968, 1017
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 3 cut(s) 511, 968, 1017
Esp3I CGTCTC 1 cut(s) 1104
FaeI CATG 6 cut(s) 155, 206, 354, 515, 617, 972
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 1065
FatI CATG 6 cut(s) 151, 202, 350, 511, 613, 968
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 9 cut(s) 75, 252, 639, 745, 748, 780, 921, 987, 1260
FokI GGATG 2 cut(s) 95, 308
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 9 cut(s) 75, 252, 639, 745, 748, 780, 921, 987, 1260
FspBI CTAG 1 cut(s) 776
GlaI GCGC 1 cut(s) 391
GluI GCNGC 9 cut(s) 75, 252, 639, 745, 748, 780, 921, 987, 1260
GsaI CCCAGC 3 cut(s) 645, 742, 1236
GsuI CTGGAG 1 cut(s) 1135
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 8 cut(s) 133, 439, 510, 837, 920, 1218, 1244, 1307
HapII CCGG 1 cut(s) 1078
HgaI GACGC 1 cut(s) 1121
HhaI GCGC 1 cut(s) 392
Hin1II CATG 6 cut(s) 155, 206, 354, 515, 617, 972
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 2 cut(s) 178, 811
HindII GTYRAC 2 cut(s) 178, 811
HinfI GANTC 4 cut(s) 443, 590, 602, 1053
HpaII CCGG 1 cut(s) 1078
HphI GGTGA 1 cut(s) 1006
Hpy166II GTNNAC 3 cut(s) 178, 727, 811
Hpy188I TCNGA 7 cut(s) 365, 448, 545, 850, 939, 1275, 1336
Hpy188III TCNNGA 4 cut(s) 80, 371, 567, 1152
Hpy8I GTNNAC 3 cut(s) 178, 727, 811
Hpy99I CGWCG 1 cut(s) 1336
HpyAV CCTTC 3 cut(s) 32, 583, 926
HpyCH4III ACNGT 8 cut(s) 182, 379, 552, 560, 652, 806, 952, 1037
HpyCH4IV ACGT 3 cut(s) 715, 1065, 1331
HpyCH4V TGCA 7 cut(s) 39, 200, 254, 872, 965, 1259, 1324
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 265, 635, 663, 719, 744
HpyF3I CTNAG 2 cut(s) 70, 78
HpySE526I ACGT 3 cut(s) 715, 1065, 1331
Hsp92II CATG 6 cut(s) 155, 206, 354, 515, 617, 972
HspAI GCGC 1 cut(s) 390
KpnI GGTACC 1 cut(s) 707
Kzo9I GATC 5 cut(s) 145, 208, 537, 569, 862
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 7 cut(s) 61, 238, 650, 756, 759, 973, 1271
LweI GCATC 7 cut(s) 73, 451, 638, 754, 759, 781, 1159
MaeI CTAG 1 cut(s) 776
MaeII ACGT 3 cut(s) 715, 1065, 1331
MaeIII GTNAC 5 cut(s) 61, 527, 926, 952, 1031
MalI GATC 5 cut(s) 147, 210, 539, 571, 864
MboI GATC 5 cut(s) 145, 208, 537, 569, 862
MboII GAAGA 8 cut(s) 140, 209, 218, 304, 593, 773, 1193, 1354
MfeI CAATTG 2 cut(s) 873, 1025
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 135, 550
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 7 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 4 cut(s) 452, 596, 599, 1047
MmeI TCCRAC 3 cut(s) 426, 811, 1314
MnlI CCTC 7 cut(s) 41, 486, 689, 692, 827, 1042, 1085
Mox20I TGGCCA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 439
MseI TTAA 2 cut(s) 1097, 1191
Msp20I TGGCCA 1 cut(s) 439
MspA1I CMGCKG 4 cut(s) 74, 641, 923, 1262
MspI CCGG 1 cut(s) 1078
MspR9I CCNGG 1 cut(s) 1079
MunI CAATTG 2 cut(s) 873, 1025
Mva1269I GAATGC 1 cut(s) 782
MwoI GCNNNNNNNGC 6 cut(s) 83, 265, 635, 663, 719, 744
NciI CCSGG 1 cut(s) 1079
NcoI CCATGG 2 cut(s) 511, 968
NdeII GATC 5 cut(s) 145, 208, 537, 569, 862
NlaIII CATG 6 cut(s) 155, 206, 354, 515, 617, 972
NlaIV GGNNCC 3 cut(s) 133, 705, 1243
PaqCI CACCTGC 1 cut(s) 1121
PceI AGGCCT 1 cut(s) 837
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 782
PflFI GACNNNGTC 1 cut(s) 806
PflMI CCANNNNNTGG 1 cut(s) 489
PkrI GCNGC 9 cut(s) 76, 253, 640, 746, 749, 781, 922, 988, 1261
PleI GAGTC 4 cut(s) 451, 596, 598, 1047
PpsI GAGTC 4 cut(s) 451, 596, 598, 1047
Ppu21I YACGTR 1 cut(s) 716
PspFI CCCAGC 3 cut(s) 641, 738, 1232
PspN4I GGNNCC 3 cut(s) 133, 705, 1243
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 7 cut(s) 131, 132, 473, 935, 1217, 1242, 1306
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 3 cut(s) 74, 641, 1262
RsaI GTAC 2 cut(s) 705, 728
RsaNI GTAC 2 cut(s) 704, 727
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 1097, 1191
SatI GCNGC 9 cut(s) 75, 252, 639, 745, 748, 780, 921, 987, 1260
Sau3AI GATC 5 cut(s) 145, 208, 537, 569, 862
Sau96I GGNCC 7 cut(s) 131, 132, 473, 935, 1217, 1242, 1306
SchI GAGTC 4 cut(s) 452, 596, 599, 1047
ScrFI CCNGG 1 cut(s) 1079
SduI GDGCHC 2 cut(s) 135, 550
SfaNI GCATC 7 cut(s) 73, 451, 638, 754, 759, 781, 1159
SfcI CTRYAG 1 cut(s) 164
SinI GGWCC 2 cut(s) 473, 935
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
Sse9I AATT 7 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266
SseBI AGGCCT 1 cut(s) 837
SsiI CCGC 5 cut(s) 609, 627, 779, 921, 1003
SspI AATATT 1 cut(s) 43
SspMI CTAG 1 cut(s) 776
StuI AGGCCT 1 cut(s) 837
StyD4I CCNGG 1 cut(s) 1077
StyI CCWWGG 3 cut(s) 511, 968, 1017
TaaI ACNGT 8 cut(s) 182, 379, 552, 560, 652, 806, 952, 1037
TaiI ACGT 3 cut(s) 718, 1068, 1334
TaqI TCGA 6 cut(s) 10, 57, 568, 786, 810, 1224
TasI AATT 7 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266
TatI WGTACW 1 cut(s) 726
TauI GCSGC 2 cut(s) 782, 923
Tru1I TTAA 2 cut(s) 1097, 1191
Tru9I TTAA 2 cut(s) 1097, 1191
TscAI CASTG 1 cut(s) 657
TseI GCWGC 7 cut(s) 74, 251, 638, 744, 747, 986, 1259
TspDTI ATGAA 4 cut(s) 140, 219, 1000, 1310
TspRI CASTG 1 cut(s) 657
Tth111I GACNNNGTC 1 cut(s) 806
Van91I CCANNNNNTGG 1 cut(s) 489
VpaK11BI GGWCC 2 cut(s) 473, 935
XapI RAATTY 2 cut(s) 432, 1085
XcmI CCANNNNNNNNNTGG 2 cut(s) 486, 1024
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.