Rh1BG155800

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000390
Physical Location & Seq
Reverse (-)
25399756 .. 25403069
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG155800.1

Sequence Viewer

Length: 1632 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTCTACTTCTTGTTCATGTTACTCACTTAGCTGCTCGGGATGACCGGAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACCAATGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCGACCTTGAAAAGGATATCATCATCGGTGTGATCGACAGTGGAATTTGGCCAGAATCACACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTTCTGTTCAAGAAATAGCTTAAAAGAAGATTCGATCATAGGTAAAATTGTGCTTTGCGATAGAGGCCCCGATGGCAGTGGGGATGGGGATGGGGCCATAGACGGTATTGCAACCGGAGTTATTCTGACGGGCGTAAAAGTTGCCGAGGAGCTGGTTGGCCCTCTTCCCCTGCCTGCATCTCACGTCGGATTGGAAGAAAACAGCCACATTTACAAATACATAAGGGAAACAAGGAACCCAATTGCAACTATTTTCAAAAGTGAAGAGATTAATGATGTTTTGGCTCCATACGTTCCCTCCTACTCGTCAAGGGGTCCAAATCCGATGAATCCTAACATTCTCAAGCCAGATGTAGCAGCTCCAGGTACTCACATTTTAGCAGCATATGATCATCAACCGGTTGGTGGTGGCAGTAGAGTTGAGTATAATTTGGATACTGGGACATCAATGGCATGCCCCCATGCTGCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCAGCTGCTATTCAATCGGCTCTCATCACTACAGCTAAACCTATGAGTGCTAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATACAGTGGCAAACTATTGGAAATCATAACCAGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAACAGTCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTCATTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTCGGGCAGAAGGAATCTTTTGTTGTTACAATAAAAGGGTCAATTGAGAAATCAAATATAGTCTCTGCATCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCGATTGTTATCCATGTTATAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

543

Amino Acids

58.9

Weight (kDa)

6.76

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 3.8e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 283 - 386 2.5e-17 Subtilase family
fn3_6 PF17766 442 - 539 2.4e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 2 cut(s) 495, 1187
AccI GTMKAC 1 cut(s) 1197
AciI CCGC 1 cut(s) 1130
AclWI GGATC 3 cut(s) 1355, 1368, 1417
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 4 cut(s) 438, 1139, 1367, 1417
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 962, 1174, 1187
AgeI ACCGGT 1 cut(s) 955
AgsI TTSAA 5 cut(s) 404, 567, 814, 1082, 1353
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 3 cut(s) 919, 1186, 1274
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 2 cut(s) 165, 1564
AlwI GGATC 3 cut(s) 1355, 1368, 1417
Ama87I CYCGRG 2 cut(s) 78, 1499
AoxI GGCC 5 cut(s) 443, 622, 651, 715, 1156
ApeKI GCWGC 8 cut(s) 74, 257, 914, 938, 1022, 1034, 1073, 1290
ApoI RAATTY 4 cut(s) 438, 1139, 1367, 1417
AseI ATTAAT 3 cut(s) 192, 828, 1374
AsiGI ACCGGT 1 cut(s) 955
AspS9I GGNCC 5 cut(s) 479, 623, 651, 716, 872
AsuHPI GGTGA 1 cut(s) 1059
AvaI CYCGRG 2 cut(s) 78, 1499
AvaII GGWCC 2 cut(s) 479, 872
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 1 cut(s) 1360
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 1180, 1607
BbsI GAAGAC 2 cut(s) 310, 1375
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 8 cut(s) 61, 244, 926, 950, 1009, 1021, 1060, 1277
BccI CCATC 7 cut(s) 6, 296, 623, 635, 641, 1456, 1577
BciT130I CCWGG 3 cut(s) 921, 1188, 1276
BciVI GTATCC 2 cut(s) 985, 1238
BclI TGATCA 1 cut(s) 946
BcoDI GTCTC 2 cut(s) 165, 1564
BfaI CTAG 2 cut(s) 1125, 1173
BfmI CTRYAG 2 cut(s) 1023, 1098
BfuI GTATCC 2 cut(s) 985, 1238
BglI GCCNNNNNGGC 1 cut(s) 630
BisI GCNGC 8 cut(s) 75, 258, 915, 939, 1023, 1035, 1074, 1291
BlsI GCNGC 8 cut(s) 76, 259, 916, 940, 1024, 1036, 1075, 1292
Bme1390I CCNGG 3 cut(s) 921, 1188, 1276
Bme18I GGWCC 2 cut(s) 479, 872
BmeT110I CYCGRG 2 cut(s) 78, 1499
BmgBI CACGTC 1 cut(s) 742
BmgT120I GGNCC 5 cut(s) 479, 623, 651, 716, 872
BmrFI CCNGG 3 cut(s) 921, 1188, 1276
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 2 cut(s) 743, 1574
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 2 cut(s) 310, 1375
BpmI CTGGAG 1 cut(s) 903
BpuEI CTTGAG 2 cut(s) 389, 884
BsaBI GATNNNNATC 1 cut(s) 1613
BsaJI CCNNGG 3 cut(s) 247, 702, 1275
BsaWI WCCGGW 3 cut(s) 87, 671, 955
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 406, 495, 962, 1174, 1187
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 3 cut(s) 137, 294, 1000
Bse3DI GCAATG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 1613
BseBI CCWGG 3 cut(s) 921, 1188, 1276
BseDI CCNNGG 3 cut(s) 247, 702, 1275
BseGI GGATG 5 cut(s) 88, 307, 646, 652, 1585
BseJI GATNNNNATC 1 cut(s) 1613
BseLI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 962, 1174, 1187
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 3 cut(s) 137, 294, 1000
BseRI GAGGAG 1 cut(s) 719
BseXI GCAGC 8 cut(s) 61, 244, 926, 950, 1009, 1021, 1060, 1277
BshFI GGCC 5 cut(s) 445, 624, 653, 717, 1158
BshNI GGYRCC 1 cut(s) 133
BshTI ACCGGT 1 cut(s) 955
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 78, 1499
BsiSI CCGG 3 cut(s) 88, 672, 956
BslFI GGGAC 2 cut(s) 1012, 1292
BslI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 962, 1174, 1187
BsmAI GTCTC 2 cut(s) 165, 1564
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 1012, 1292
BsnI GGCC 5 cut(s) 445, 624, 653, 717, 1158
BsoBI CYCGRG 2 cut(s) 78, 1499
Bsp1286I GDGCHC 3 cut(s) 556, 1180, 1607
Bsp143I GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 1 cut(s) 1130
BspANI GGCC 5 cut(s) 445, 624, 653, 717, 1158
BspCNI CTCAG 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 1027
BspPI GGATC 3 cut(s) 1355, 1368, 1417
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 955
BsrI ACTGG 3 cut(s) 137, 294, 1000
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 3 cut(s) 247, 702, 1275
BssMI GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
BssNAI GTATAC 1 cut(s) 1198
BssT1I CCWWGG 1 cut(s) 247
Bst1107I GTATAC 1 cut(s) 1198
Bst2UI CCWGG 3 cut(s) 921, 1188, 1276
Bst4CI ACNGT 7 cut(s) 385, 434, 558, 662, 1250, 1318, 1396
Bst6I CTCTTC 3 cut(s) 12, 726, 816
BstC8I GCNNGC 6 cut(s) 732, 1012, 1027, 1130, 1134, 1156
BstDEI CTNAG 2 cut(s) 26, 70
BstDSI CCRYGG 1 cut(s) 247
BstEII GGTNACC 1 cut(s) 1065
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 5 cut(s) 88, 307, 646, 652, 1585
BstKTI GATC 7 cut(s) 151, 429, 594, 949, 1204, 1363, 1412
BstMAI GTCTC 2 cut(s) 165, 1564
BstMBI GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
BstMWI GCNNNNNNNGC 7 cut(s) 36, 212, 621, 630, 1031, 1142, 1151
BstNI CCWGG 3 cut(s) 921, 1188, 1276
BstNSI RCATGY 1 cut(s) 1014
BstPI GGTNACC 1 cut(s) 1065
BstSCI CCNGG 3 cut(s) 919, 1186, 1274
BstSFI CTRYAG 2 cut(s) 1023, 1098
BstV1I GCAGC 8 cut(s) 61, 244, 926, 950, 1009, 1021, 1060, 1277
BstV2I GAAGAC 2 cut(s) 310, 1375
BstX2I RGATCY 1 cut(s) 1360
BstYI RGATCY 1 cut(s) 1360
BstZ17I GTATAC 1 cut(s) 1198
BsuI GTATCC 2 cut(s) 985, 1238
BsuRI GGCC 5 cut(s) 445, 624, 653, 717, 1158
BtgI CCRYGG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 5 cut(s) 88, 307, 646, 652, 1585
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 3 cut(s) 439, 640, 1255
Cac8I GCNNGC 6 cut(s) 732, 1012, 1027, 1130, 1134, 1156
Cfr10I RCCGGY 1 cut(s) 955
Cfr13I GGNCC 5 cut(s) 479, 623, 651, 716, 872
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 924
CspAI ACCGGT 1 cut(s) 955
CviAII CATG 8 cut(s) 59, 155, 209, 248, 357, 1011, 1019, 1619
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 2 cut(s) 26, 70
DpnI GATC 7 cut(s) 150, 428, 593, 948, 1203, 1362, 1411
DpnII GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 3 cut(s) 12, 726, 816
EarI CTCTTC 3 cut(s) 12, 726, 816
Eco130I CCWWGG 1 cut(s) 247
Eco24I GRGCYC 2 cut(s) 1180, 1607
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 479, 872
Eco88I CYCGRG 2 cut(s) 78, 1499
Eco91I GGTNACC 1 cut(s) 1065
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 1065
EcoRI GAATTC 2 cut(s) 1367, 1417
EcoRII CCWGG 3 cut(s) 919, 1186, 1274
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 247
EcoT38I GRGCYC 2 cut(s) 1180, 1607
ErhI CCWWGG 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 8 cut(s) 62, 158, 212, 251, 360, 1014, 1022, 1622
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 2 cut(s) 1012, 1292
FatI CATG 8 cut(s) 58, 154, 208, 247, 356, 1010, 1018, 1618
FauI CCCGC 1 cut(s) 1137
FauNDI CATATG 3 cut(s) 506, 943, 1147
FbaI TGATCA 1 cut(s) 946
FblI GTMKAC 1 cut(s) 1197
Fnu4HI GCNGC 8 cut(s) 75, 258, 915, 939, 1023, 1035, 1074, 1291
FokI GGATG 5 cut(s) 95, 314, 653, 659, 1592
FriOI GRGCYC 2 cut(s) 1180, 1607
Fsp4HI GCNGC 8 cut(s) 75, 258, 915, 939, 1023, 1035, 1074, 1291
FspBI CTAG 2 cut(s) 1125, 1173
GluI GCNGC 8 cut(s) 75, 258, 915, 939, 1023, 1035, 1074, 1291
GsuI CTGGAG 1 cut(s) 903
HaeIII GGCC 5 cut(s) 445, 624, 653, 717, 1158
HapII CCGG 3 cut(s) 88, 672, 956
HgaI GACGC 1 cut(s) 328
Hin1II CATG 8 cut(s) 62, 158, 212, 251, 360, 1014, 1022, 1622
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 6 cut(s) 449, 587, 886, 1455, 1471, 1511
HpaII CCGG 3 cut(s) 88, 672, 956
HphI GGTGA 1 cut(s) 1059
Hpy166II GTNNAC 3 cut(s) 181, 1198, 1238
Hpy188I TCNGA 4 cut(s) 551, 684, 746, 882
Hpy188III TCNNGA 7 cut(s) 80, 377, 567, 1205, 1364, 1452, 1600
Hpy8I GTNNAC 3 cut(s) 181, 1198, 1238
Hpy99I CGWCG 2 cut(s) 746, 1417
HpyAV CCTTC 5 cut(s) 32, 85, 1179, 1347, 1501
HpyCH4III ACNGT 7 cut(s) 385, 434, 558, 662, 1250, 1318, 1396
HpyCH4IV ACGT 4 cut(s) 174, 741, 849, 1041
HpyCH4V TGCA 9 cut(s) 206, 260, 668, 734, 803, 1025, 1037, 1145, 1565
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 212, 621, 630, 1031, 1142, 1151
HpyF3I CTNAG 2 cut(s) 26, 70
HpySE526I ACGT 4 cut(s) 174, 741, 849, 1041
Hsp92II CATG 8 cut(s) 62, 158, 212, 251, 360, 1014, 1022, 1622
Ksp22I TGATCA 1 cut(s) 946
Kzo9I GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 8 cut(s) 551, 706, 847, 922, 1151, 1183, 1450, 1602
Lsp1109I GCAGC 8 cut(s) 61, 244, 926, 950, 1009, 1021, 1060, 1277
LweI GCATC 2 cut(s) 743, 1574
MaeI CTAG 2 cut(s) 1125, 1173
MaeII ACGT 4 cut(s) 174, 741, 849, 1041
MaeIII GTNAC 6 cut(s) 61, 139, 533, 1065, 1396, 1522
MalI GATC 7 cut(s) 150, 428, 593, 948, 1203, 1362, 1411
MboI GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
MboII GAAGA 8 cut(s) 143, 215, 310, 596, 713, 764, 833, 1375
MfeI CAATTG 2 cut(s) 798, 1539
MflI RGATCY 1 cut(s) 1360
MhlI GDGCHC 3 cut(s) 556, 1180, 1607
MlsI TGGCCA 1 cut(s) 445
MluCI AATT 9 cut(s) 438, 543, 603, 798, 985, 1139, 1367, 1417, 1539
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 1 cut(s) 1449
MmeI TCCRAC 2 cut(s) 724, 1387
MnlI CCTC 6 cut(s) 41, 492, 614, 697, 729, 865
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 6 cut(s) 192, 387, 578, 828, 1374, 1630
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 3 cut(s) 88, 672, 956
MspR9I CCNGG 3 cut(s) 921, 1188, 1276
MunI CAATTG 2 cut(s) 798, 1539
MvaI CCWGG 3 cut(s) 921, 1188, 1276
MwoI GCNNNNNNNGC 7 cut(s) 36, 212, 621, 630, 1031, 1142, 1151
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 3 cut(s) 506, 943, 1147
NdeII GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
NlaIII CATG 8 cut(s) 62, 158, 212, 251, 360, 1014, 1022, 1622
NmeAIII GCCGAG 1 cut(s) 727
NmuCI GTSAC 2 cut(s) 1065, 1396
NspI RCATGY 1 cut(s) 1014
PaeI GCATGC 1 cut(s) 1014
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PfeI GAWTC 5 cut(s) 449, 587, 886, 1471, 1511
PflMI CCANNNNNTGG 2 cut(s) 495, 1187
PinAI ACCGGT 1 cut(s) 955
PkrI GCNGC 8 cut(s) 76, 259, 916, 940, 1024, 1036, 1075, 1292
PleI GAGTC 1 cut(s) 1449
PpsI GAGTC 1 cut(s) 1449
PshBI ATTAAT 3 cut(s) 192, 828, 1374
Psp6I CCWGG 3 cut(s) 919, 1186, 1274
PspEI GGTNACC 1 cut(s) 1065
PspGI CCWGG 3 cut(s) 919, 1186, 1274
PspPI GGNCC 5 cut(s) 479, 623, 651, 716, 872
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 1360
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 422
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 192, 387, 578, 828, 1374, 1630
SatI GCNGC 8 cut(s) 75, 258, 915, 939, 1023, 1035, 1074, 1291
Sau3AI GATC 7 cut(s) 148, 426, 591, 946, 1201, 1360, 1409
Sau96I GGNCC 5 cut(s) 479, 623, 651, 716, 872
SchI GAGTC 1 cut(s) 1449
ScrFI CCNGG 3 cut(s) 921, 1188, 1276
SduI GDGCHC 3 cut(s) 556, 1180, 1607
SfaNI GCATC 2 cut(s) 743, 1574
SfcI CTRYAG 2 cut(s) 1023, 1098
SinI GGWCC 2 cut(s) 479, 872
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 368, 899
SmoI CTYRAG 2 cut(s) 368, 899
SphI GCATGC 1 cut(s) 1014
Sse9I AATT 9 cut(s) 438, 543, 603, 798, 985, 1139, 1367, 1417, 1539
SsiI CCGC 1 cut(s) 1130
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 1125, 1173
StyD4I CCNGG 3 cut(s) 919, 1186, 1274
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 7 cut(s) 385, 434, 558, 662, 1250, 1318, 1396
TaiI ACGT 4 cut(s) 177, 744, 852, 1044
TaqI TCGA 4 cut(s) 10, 429, 590, 1415
TasI AATT 9 cut(s) 438, 543, 603, 798, 985, 1139, 1367, 1417, 1539
TfiI GAWTC 5 cut(s) 449, 587, 886, 1471, 1511
Tru1I TTAA 6 cut(s) 192, 387, 578, 828, 1374, 1630
Tru9I TTAA 6 cut(s) 192, 387, 578, 828, 1374, 1630
TscAI CASTG 3 cut(s) 439, 640, 1255
TseFI GTSAC 2 cut(s) 1065, 1396
TseI GCWGC 8 cut(s) 74, 257, 914, 938, 1022, 1034, 1073, 1290
Tsp45I GTSAC 2 cut(s) 1065, 1396
TspDTI ATGAA 7 cut(s) 47, 143, 225, 899, 1224, 1326, 1360
TspRI CASTG 3 cut(s) 439, 640, 1255
Van91I CCANNNNNTGG 2 cut(s) 495, 1187
VpaK11BI GGWCC 2 cut(s) 479, 872
VspI ATTAAT 3 cut(s) 192, 828, 1374
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 4 cut(s) 438, 1139, 1367, 1417
XceI RCATGY 1 cut(s) 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmiI GTMKAC 1 cut(s) 1197
XspI CTAG 2 cut(s) 1125, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.