RLG00000027140

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Forward (+)
8905913 .. 8911244
5332 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027140

Sequence Viewer

Length: 2229 bp
ATGGCTCTTCGATGGTTTCTCCTCCTTAGCCTCATTGGCAGTATTCTATTTCTTGTTGATGTTACTCACTCAGCTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGTGACAAGCCAAAGAACGGGATGCCAGTAACACCTGATCTTCATGTAAATATTCTACGAGACATAGTTGATAGCAGCATTAATAAGGATATTGCACATGAAACTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCACGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTGTCTGTCTTTCCAAGTAAAAAGGTGAGCGTCAAAACAACAACGTCATGGAATTTCGTTGGGTTTCCAGAATCAATTGGGAGTGGCCCCTTTGAAAGTGATATCATTATCGGTGTGCTCGACAGTGGAATTTGGCCGGAGTTGGACAGCTTTAATGATGTTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTCACTTGTAACAATAAAATCATCGGAGCACGGTATTACAAATCTAGTGACTTCGAAGAAGAAGATATTCCGTCTCCAAGAGACGCATCCGGTCATGGAACCCACTGCGCGTCAATAGCAGCTGGGAATGCAGTGAGCAACGCGAGCATAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGCGGTGCCATCAGCACGTATTGCGGTGTACAAAATATGTTGGGACAAAGGTTGTGGTGTTGCTGAGTTTCTAGCGGCAATCGACGATGCCATTGCTGACGGTGTCGACATTATCAGTGCTTCCACAGGAGGTGCTGCGCCAGCTGACTTTTTGAATGACCCATTTGCAATTGGGGCATTTCATGCTACCGTAAATGGGATATTGGTTTCTATGGCCGCTGGTAATGAAGGTCCGCAAAAAAAAACTGTGGAAAACATTGCACCATGGCAACTTTCTGTGGCTGCTACCACCACAAACCGCCAGTTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTCCGTACATATACATGACCTCCAAGGTAAATTTTACCCGTTGATTTACGGTGGAGACGCACCCAATAAAGAAGATGGTAAAGGAGATGATTCTAGGTTTTGTGTTGGAGATAGCTTAGATGCAGATAAGATCAAAGGTAAAATTGTGCTTTGTGATCGCCCCGATAAGGATGGGAAGGGGGCTGGGGATGGTGCCAAATGGTGGAACGCTTCAATGGGAGTTATTCTGACGGGCAAACAAGTTGTCGAGGAAACCGAATATGCTGATAACCCTTGTGCAGCATCTTACGTCAATTTAGAAGATAATAGCAACATTTACAAATACATAAACAAAACAAGGGACCCAACTGCAACTATTTTGAAAAGTGAAGAGATCAATGATGTATTGTCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAACGCGTTGAATCCCAACATACTGAAGCCAGATTTAGCGGCTCCGGGAACTTACATTCTAGCAGCATATCCCCCAAATGTTAATAATGGAGCTAAATACAAATTGGCAACCGGCACATCAATGGCATGCCCCCATGCTTCGTCTGTAGCTGCGTACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTGTCCAATCGGCTCTCATCACTACTGCTACGCCTATGAGTGACAAAACTACCCCGCACGCTGAAATTGCATATGGAGCTGGCCTAATAAACCCTTCCAGAGCTCCATATCCTGGTTTGGTTTACGATCTTGATGTACAAGACTATGTAGATTTTTTGTGTTCACAAGGATACAGTGACAAACTATTAGAACAAATAACCAGGGACAAGACTACCTCATGCTCATCAAAATATGATAATGAAACAGTCAATGACCTGAATTATCCTTCTTTTGCTCTTCCTATCAAGGATCCGAAATTTGTCAATGGAGTTTTCCATAGGACTGTCACTAATGTCGGATCATCAAATTCTATATACAGAGCTAAAGTGGTGGCTCCGTCGGGACTCAAAATCAATGTGGATCCAAGTGTGTTGTCGTTCACATCTCTTGAGCAAAAGAAATCTTTTGTAGTCACTGTAAAAGGGCCGATTGAGAAAACAAGTATGGTCTCTGCGTCTTTGGTGTGGGACGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATATTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

743

Amino Acids

79.69

Weight (kDa)

6.0

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 114 1.1e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 584 4.4e-44 Subtilase family
fn3_6 PF17766 641 - 738 2.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 709, 1247
AccB7I CCANNNNNTGG 2 cut(s) 1257, 1781
AccBSI CCGCTC 1 cut(s) 707
AccI GTMKAC 1 cut(s) 810
AccII CGCG 3 cut(s) 635, 668, 1484
AciI CCGC 8 cut(s) 707, 728, 779, 921, 938, 1003, 1517, 1724
AclWI GGATC 5 cut(s) 1952, 1965, 2014, 2063, 2076
AcoI YGGCCR 2 cut(s) 443, 918
AcsI RAATTY 5 cut(s) 361, 438, 1085, 1964, 2014
AcuI CTGAAG 1 cut(s) 1523
AfaI GTAC 4 cut(s) 734, 1062, 1634, 1806
AfiI CCNNNNNNNGG 5 cut(s) 128, 900, 1222, 1257, 1781
AflIII ACRYGT 1 cut(s) 1482
AgsI TTSAA 7 cut(s) 404, 859, 1025, 1269, 1417, 1489, 2222
AjnI CCWGG 2 cut(s) 1780, 1868
Alw21I GWGCWC 3 cut(s) 429, 556, 1774
Alw26I GTCTC 6 cut(s) 165, 600, 603, 1104, 1449, 2161
AlwI GGATC 5 cut(s) 1952, 1965, 2014, 2063, 2076
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 5 cut(s) 394, 443, 918, 1750, 2132
ApoI RAATTY 5 cut(s) 361, 438, 1085, 1964, 2014
ArsI GACNNNNNNTTYG 2 cut(s) 1284, 1316
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 2 cut(s) 635, 844
AspS9I GGNCC 6 cut(s) 395, 479, 935, 1396, 1475, 2132
AsuC2I CCSGG 1 cut(s) 1524
AsuHPI GGTGA 5 cut(s) 125, 346, 1006, 1640, 1653
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 4 cut(s) 479, 935, 1396, 1475
BaeGI GKGCMC 1 cut(s) 1458
BamHI GGATCC 2 cut(s) 1957, 2068
BanI GGYRCC 2 cut(s) 709, 1247
BanII GRGCYC 2 cut(s) 1774, 2204
Bbv12I GWGCWC 3 cut(s) 429, 556, 1774
BccI CCATC 7 cut(s) 6, 296, 721, 1124, 1220, 1238, 2174
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 2 cut(s) 1782, 1870
BciVI GTATCC 1 cut(s) 1832
BcnI CCSGG 1 cut(s) 1524
BcoDI GTCTC 6 cut(s) 165, 600, 603, 1104, 1449, 2161
BfaI CTAG 4 cut(s) 570, 776, 1149, 1538
BfmI CTRYAG 2 cut(s) 1623, 2225
BfuI GTATCC 1 cut(s) 1832
BglI GCCNNNNNGGC 2 cut(s) 36, 92
Bme1390I CCNGG 3 cut(s) 1524, 1782, 1870
Bme18I GGWCC 4 cut(s) 479, 935, 1396, 1475
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 6 cut(s) 395, 479, 935, 1396, 1475, 2132
BmrFI CCNGG 3 cut(s) 1524, 1782, 1870
BmsI GCATC 6 cut(s) 73, 123, 620, 781, 1165, 1346
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 2117
BpuMI CCSGG 1 cut(s) 1524
BsaAI YACGTR 2 cut(s) 722, 1453
BsaI GGTCTC 1 cut(s) 2161
BsaJI CCNNGG 5 cut(s) 247, 968, 1017, 1078, 1869
BsaWI WCCGGW 1 cut(s) 614
BsaXI ACNNNNNCTCC 4 cut(s) 1059, 1089, 1442, 1472
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 900, 1222, 1257, 1781
Bse118I RCCGGY 2 cut(s) 1589, 1663
Bse1I ACTGG 3 cut(s) 137, 294, 1006
Bse3DI GCAATG 3 cut(s) 267, 795, 960
BseBI CCWGG 2 cut(s) 1782, 1870
BseDI CCNNGG 5 cut(s) 247, 968, 1017, 1078, 1869
BseGI GGATG 6 cut(s) 88, 138, 307, 611, 1231, 1249
BseLI CCNNNNNNNGG 5 cut(s) 128, 900, 1222, 1257, 1781
BseMI GCAATG 3 cut(s) 267, 795, 960
BseMII CTCAG 2 cut(s) 84, 759
BseNI ACTGG 3 cut(s) 137, 294, 1006
BseRI GAGGAG 2 cut(s) 11, 717
BseSI GKGCMC 1 cut(s) 1458
BseYI CCCAGC 2 cut(s) 647, 1238
BsgI GTGCAG 1 cut(s) 1353
Bsh1236I CGCG 3 cut(s) 635, 668, 1484
BshFI GGCC 5 cut(s) 396, 445, 920, 1752, 2134
BshNI GGYRCC 2 cut(s) 709, 1247
BsiHKAI GWGCWC 3 cut(s) 429, 556, 1774
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 5 cut(s) 446, 615, 1523, 1590, 1664
BsiWI CGTACG 1 cut(s) 1632
BslFI GGGAC 6 cut(s) 761, 1065, 1409, 1886, 2064, 2189
BslI CCNNNNNNNGG 5 cut(s) 128, 900, 1222, 1257, 1781
BsmAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1449, 2161
BsmBI CGTCTC 3 cut(s) 600, 603, 1104
BsmFI GGGAC 6 cut(s) 761, 1065, 1409, 1886, 2064, 2189
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 5 cut(s) 396, 445, 920, 1752, 2134
Bso31I GGTCTC 1 cut(s) 2161
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 5 cut(s) 429, 556, 1458, 1774, 2204
Bsp1407I TGTACA 2 cut(s) 732, 1804
Bsp143I GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
Bsp19I CCATGG 1 cut(s) 968
BspACI CCGC 8 cut(s) 707, 728, 779, 921, 938, 1003, 1517, 1724
BspANI GGCC 5 cut(s) 396, 445, 920, 1752, 2134
BspCNI CTCAG 2 cut(s) 83, 760
BspFNI CGCG 3 cut(s) 635, 668, 1484
BspPI GGATC 5 cut(s) 1952, 1965, 2014, 2063, 2076
BspQI GCTCTTC 2 cut(s) 12, 1950
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 709, 1247
BspTNI GGTCTC 1 cut(s) 2161
BsrBI CCGCTC 1 cut(s) 707
BsrDI GCAATG 3 cut(s) 267, 795, 960
BsrFI RCCGGY 2 cut(s) 1589, 1663
BsrGI TGTACA 2 cut(s) 732, 1804
BsrI ACTGG 3 cut(s) 137, 294, 1006
BssAI RCCGGY 2 cut(s) 1589, 1663
BssECI CCNNGG 5 cut(s) 247, 968, 1017, 1078, 1869
BssMI GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
BssT1I CCWWGG 3 cut(s) 968, 1017, 1078
Bst2UI CCWGG 2 cut(s) 1782, 1870
Bst6I CTCTTC 3 cut(s) 12, 1419, 1950
BstAPI GCANNNNNTGC 2 cut(s) 725, 887
BstAUI TGTACA 2 cut(s) 732, 1804
BstBAI YACGTR 2 cut(s) 722, 1453
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 5 cut(s) 670, 846, 1606, 1728, 1750
BstDEI CTNAG 5 cut(s) 26, 70, 489, 768, 1171
BstDSI CCRYGG 2 cut(s) 247, 968
BstEII GGTNACC 1 cut(s) 1659
BstF5I GGATG 6 cut(s) 88, 138, 307, 611, 1231, 1249
BstFNI CGCG 3 cut(s) 635, 668, 1484
BstHHI GCGC 2 cut(s) 635, 844
BstKTI GATC 8 cut(s) 151, 1188, 1213, 1431, 1798, 1960, 2009, 2071
BstMAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1449, 2161
BstMBI GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
BstNI CCWGG 2 cut(s) 1782, 1870
BstNSI RCATGY 1 cut(s) 1608
BstPI GGTNACC 1 cut(s) 1659
BstSCI CCNGG 3 cut(s) 1522, 1780, 1868
BstSFI CTRYAG 2 cut(s) 1623, 2225
BstSLI GKGCMC 1 cut(s) 1458
BstUI CGCG 3 cut(s) 635, 668, 1484
BstX2I RGATCY 2 cut(s) 1957, 2068
BstYI RGATCY 2 cut(s) 1957, 2068
BsuI GTATCC 1 cut(s) 1832
BsuRI GGCC 5 cut(s) 396, 445, 920, 1752, 2134
BtgI CCRYGG 2 cut(s) 247, 968
BtsCI GGATG 6 cut(s) 88, 138, 307, 611, 1231, 1249
BtsI GCAGTG 2 cut(s) 628, 663
BtsIMutI CAGTG 6 cut(s) 439, 628, 663, 826, 1849, 2121
Cac8I GCNNGC 5 cut(s) 670, 846, 1606, 1728, 1750
CfoI GCGC 2 cut(s) 635, 844
Cfr10I RCCGGY 2 cut(s) 1589, 1663
Cfr13I GGNCC 6 cut(s) 395, 479, 935, 1396, 1475, 2132
CseI GACGC 5 cut(s) 328, 617, 624, 1121, 2151
Csp6I GTAC 4 cut(s) 733, 1061, 1633, 1805
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviQI GTAC 4 cut(s) 733, 1061, 1633, 1805
DdeI CTNAG 5 cut(s) 26, 70, 489, 768, 1171
DpnI GATC 8 cut(s) 150, 1187, 1212, 1430, 1797, 1959, 2008, 2070
DpnII GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
EaeI YGGCCR 2 cut(s) 443, 918
Eam1104I CTCTTC 3 cut(s) 12, 1419, 1950
EarI CTCTTC 3 cut(s) 12, 1419, 1950
Ecl136II GAGCTC 1 cut(s) 1772
Eco130I CCWWGG 3 cut(s) 968, 1017, 1078
Eco24I GRGCYC 2 cut(s) 1774, 2204
Eco31I GGTCTC 1 cut(s) 2161
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 4 cut(s) 479, 935, 1396, 1475
Eco53kI GAGCTC 1 cut(s) 1772
Eco57I CTGAAG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1659
EcoICRI GAGCTC 1 cut(s) 1772
EcoO109I RGGNCCY 1 cut(s) 1396
EcoO65I GGTNACC 1 cut(s) 1659
EcoRII CCWGG 2 cut(s) 1780, 1868
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 968, 1017, 1078
EcoT38I GRGCYC 2 cut(s) 1774, 2204
ErhI CCWWGG 3 cut(s) 968, 1017, 1078
Esp3I CGTCTC 3 cut(s) 600, 603, 1104
FaqI GGGAC 6 cut(s) 761, 1065, 1409, 1886, 2064, 2189
FauI CCCGC 1 cut(s) 1731
FauNDI CATATG 2 cut(s) 506, 1741
FblI GTMKAC 1 cut(s) 810
FokI GGATG 6 cut(s) 95, 145, 314, 598, 1238, 1256
FriOI GRGCYC 2 cut(s) 1774, 2204
FspBI CTAG 4 cut(s) 570, 776, 1149, 1538
GlaI GCGC 2 cut(s) 634, 843
GsaI CCCAGC 2 cut(s) 651, 1242
HaeIII GGCC 5 cut(s) 396, 445, 920, 1752, 2134
HapII CCGG 5 cut(s) 446, 615, 1523, 1590, 1664
HgaI GACGC 5 cut(s) 328, 617, 624, 1121, 2151
HhaI GCGC 2 cut(s) 635, 844
Hin6I GCGC 2 cut(s) 633, 842
HinP1I GCGC 2 cut(s) 633, 842
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 4 cut(s) 380, 1145, 1489, 2052
HpaII CCGG 5 cut(s) 446, 615, 1523, 1590, 1664
HphI GGTGA 5 cut(s) 125, 346, 1006, 1640, 1653
Hpy166II GTNNAC 5 cut(s) 733, 811, 1792, 1832, 2088
Hpy188I TCNGA 4 cut(s) 551, 1284, 1962, 2006
Hpy188III TCNNGA 7 cut(s) 80, 377, 1767, 1799, 2049, 2096, 2197
Hpy8I GTNNAC 5 cut(s) 733, 811, 1792, 1832, 2088
Hpy99I CGWCG 2 cut(s) 791, 2050
HpyAV CCTTC 4 cut(s) 926, 1225, 1773, 1944
HpyCH4IV ACGT 5 cut(s) 353, 721, 1344, 1452, 1635
HpyCH4V TGCA 9 cut(s) 206, 260, 656, 872, 965, 1178, 1334, 1406, 1739
HpyF3I CTNAG 5 cut(s) 26, 70, 489, 768, 1171
HpySE526I ACGT 5 cut(s) 353, 721, 1344, 1452, 1635
HspAI GCGC 2 cut(s) 633, 842
KflI GGGWCCC 1 cut(s) 1396
Kzo9I GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
LguI GCTCTTC 2 cut(s) 12, 1950
LmnI GCTCC 8 cut(s) 551, 704, 1525, 1568, 1745, 1777, 2047, 2199
LweI GCATC 6 cut(s) 73, 123, 620, 781, 1165, 1346
MaeI CTAG 4 cut(s) 570, 776, 1149, 1538
MaeII ACGT 5 cut(s) 353, 721, 1344, 1452, 1635
MalI GATC 8 cut(s) 150, 1187, 1212, 1430, 1797, 1959, 2008, 2070
MbiI CCGCTC 1 cut(s) 707
MboI GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
MboII GAAGA 9 cut(s) 143, 215, 593, 596, 599, 1139, 1367, 1436, 1937
MfeI CAATTG 3 cut(s) 384, 873, 1025
MflI RGATCY 2 cut(s) 1957, 2068
MhlI GDGCHC 5 cut(s) 429, 556, 1458, 1774, 2204
MluI ACGCGT 1 cut(s) 1482
MlyI GAGTC 1 cut(s) 2046
MmeI TCCRAC 3 cut(s) 432, 1141, 1984
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 3 cut(s) 192, 462, 1560
MslI CAYNNNNRTG 2 cut(s) 1068, 1598
MspA1I CMGCKG 4 cut(s) 74, 647, 848, 923
MspI CCGG 5 cut(s) 446, 615, 1523, 1590, 1664
MspR9I CCNGG 3 cut(s) 1524, 1782, 1870
MunI CAATTG 3 cut(s) 384, 873, 1025
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 2 cut(s) 1782, 1870
MvnI CGCG 3 cut(s) 635, 668, 1484
NciI CCSGG 1 cut(s) 1524
NcoI CCATGG 1 cut(s) 968
NdeI CATATG 2 cut(s) 506, 1741
NdeII GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
NmuCI GTSAC 7 cut(s) 113, 572, 1659, 1709, 1844, 1993, 2119
NspI RCATGY 1 cut(s) 1608
NspV TTCGAA 1 cut(s) 579
PaeI GCATGC 1 cut(s) 1608
PciSI GCTCTTC 2 cut(s) 12, 1950
PcsI WCGNNNNNNNCGW 3 cut(s) 85, 426, 1308
PctI GAATGC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 3 cut(s) 380, 1145, 1489
Pfl23II CGTACG 1 cut(s) 1632
PflFI GACNNNGTC 1 cut(s) 806
PflMI CCANNNNNTGG 2 cut(s) 1257, 1781
PfoI TCCNGGA 1 cut(s) 1522
PleI GAGTC 1 cut(s) 2046
PpsI GAGTC 1 cut(s) 2046
Ppu21I YACGTR 2 cut(s) 722, 1453
PpuMI RGGWCCY 1 cut(s) 1396
PshBI ATTAAT 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 1774
Psp5II RGGWCCY 1 cut(s) 1396
Psp6I CCWGG 2 cut(s) 1780, 1868
PspEI GGTNACC 1 cut(s) 1659
PspFI CCCAGC 2 cut(s) 647, 1238
PspGI CCWGG 2 cut(s) 1780, 1868
PspLI CGTACG 1 cut(s) 1632
PspPI GGNCC 6 cut(s) 395, 479, 935, 1396, 1475, 2132
PspPPI RGGWCCY 1 cut(s) 1396
PsuI RGATCY 2 cut(s) 1957, 2068
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 3 cut(s) 74, 647, 848
RsaI GTAC 4 cut(s) 734, 1062, 1634, 1806
RsaNI GTAC 4 cut(s) 733, 1061, 1633, 1805
RseI CAYNNNNRTG 2 cut(s) 1068, 1598
SacI GAGCTC 1 cut(s) 1774
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 2 cut(s) 12, 1950
SaqAI TTAA 3 cut(s) 192, 462, 1560
Sau3AI GATC 8 cut(s) 148, 1185, 1210, 1428, 1795, 1957, 2006, 2068
Sau96I GGNCC 6 cut(s) 395, 479, 935, 1396, 1475, 2132
SchI GAGTC 1 cut(s) 2046
ScrFI CCNGG 3 cut(s) 1524, 1782, 1870
SduI GDGCHC 5 cut(s) 429, 556, 1458, 1774, 2204
SfaNI GCATC 6 cut(s) 73, 123, 620, 781, 1165, 1346
SfcI CTRYAG 2 cut(s) 1623, 2225
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 479, 935, 1396, 1475
SmiMI CAYNNNNRTG 2 cut(s) 1068, 1598
SmlI CTYRAG 1 cut(s) 2096
SmoI CTYRAG 1 cut(s) 2096
SphI GCATGC 1 cut(s) 1608
SsiI CCGC 8 cut(s) 707, 728, 779, 921, 938, 1003, 1517, 1724
SspI AATATT 1 cut(s) 163
SspMI CTAG 4 cut(s) 570, 776, 1149, 1538
SstI GAGCTC 1 cut(s) 1774
StyD4I CCNGG 3 cut(s) 1522, 1780, 1868
StyI CCWWGG 3 cut(s) 968, 1017, 1078
TaiI ACGT 5 cut(s) 356, 724, 1347, 1455, 1638
TaqI TCGA 6 cut(s) 10, 429, 579, 786, 810, 1302
TatI WGTACW 2 cut(s) 732, 1804
TauI GCSGC 3 cut(s) 782, 923, 1520
TfiI GAWTC 3 cut(s) 380, 1145, 1489
Tru1I TTAA 3 cut(s) 192, 462, 1560
Tru9I TTAA 3 cut(s) 192, 462, 1560
TscAI CASTG 6 cut(s) 439, 635, 663, 826, 1849, 2128
TseFI GTSAC 7 cut(s) 113, 572, 1659, 1709, 1844, 1993, 2119
Tsp45I GTSAC 7 cut(s) 113, 572, 1659, 1709, 1844, 1993, 2119
TspDTI ATGAA 6 cut(s) 143, 225, 875, 945, 1000, 1923
TspGWI ACGGA 4 cut(s) 264, 585, 1048, 2034
TspRI CASTG 6 cut(s) 439, 635, 663, 826, 1849, 2128
Tth111I GACNNNGTC 1 cut(s) 806
Van91I CCANNNNNTGG 2 cut(s) 1257, 1781
VpaK11BI GGWCC 4 cut(s) 479, 935, 1396, 1475
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 5 cut(s) 361, 438, 1085, 1964, 2014
XceI RCATGY 1 cut(s) 1608
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1024
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 4 cut(s) 570, 776, 1149, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.