MD07G1207000.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28568731 .. 28572403
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1207000.v1.1.491

Sequence Viewer

Length: 2136 bp
ATGGATTATATTGTGTATATGGGTGACAAGCCAAAGCCTGAGGTTTCCACCACTACCACATCAGCTCTTCATGTAAACATGCTACAAAACGTCGTCGAGGACAGCAATATAGCGCATGAATCTCTGCTTCTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCCAGGCTAACAGAGGAAGAAGCACAGAAGTTGGCTGGAATGGATGGTGTTGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCTCCAAACAACAAGGTCATGGGACTTCATTGGGTTTCCTGAAATGGTGAAGAGAAGCGCCATTGAAACTGATACCATTGTCGGGATGATTGACTCTGGAATTTGGCCTGAATCTGCCAGCTTCAGTGACGCCGGGTTTGGTCCACCCCCCAAAAGGTGGAAAGGCGCATGCAAGGGCGAAGGCAATTTTACTTGCAACAATAAAATTATCGGAGCACGATATTACCGCAGTCAACCCTACCCCAAAAATAGCAGTGACGTCATGTCTCCGAGAGACACGGAAGGCCATGGAACCCACACTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGGGTGCCATCAGCACGCATTGCGGTGTACAAAGTTTGTTGGTCAGACGGGTGCCCGGACGCTGATATTCTAGCGGCATTCGATGATGCCATAGCTGACGGTGTTGACATACTCTCTGTCTCCCTTGGGGGCCGTAAACCGTTAGATTATTTCAGAAATGCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTCACTTCCGCGTCTGCTGGTAATGAAGGTCCAAACCTGAAAACTATTACAAACTTTGCACCATGGTCTCTTGCTGTAGCTGCTAGCACCATAGATCGCCACTTTGATACCAAGGTTCAATTGGGAAACCACAAAATTTATGAGGGAATAGTAACAAACACATTTGAACTCAAGGGTAAATTCTACCCCATAGTATATGCTGGAGACGTTCCAGATAGGGCAGCAGGTTACAATGGGGAAACATCCAGGTTATGCCAAACAGGCACGTTAGACACAAAATTGGTGGAGGATAAAATTGTTCTTTGCGATGGGCCTACTGGGGATGGGGCATTATATGCAGGCGCATTTGGTTATGTTTTGACAAGCCGAAATGCAGCAGATGTAATCGATCCTGTTCCAATACCAGCAGCTTCCGTTTGGTTCCATGTTGGTAACGAAATTACCCATTACATAAACTCAACCAGGAACCCAACAGCAACAATTTGGAAAAGTACTGAGGGTAGGGATGCACTGGCCCCATATGTACCTTCATTCTCATCAAGGGGTCCAAATCCAAACACTCCTAATATTCTCAAGCCAGATATCGCTTCTCCTGGAGTTTCCATTCTAGCAGCATGGCCTCCAATTGCCCCAGTTTCAGGTGTTGAAGGTGATGACAGAGTAGCTTCATACAATATAATCTCGGGGACATCAATGGCATGCCCACATGCTGCGGGCGTGGCTGCATACGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTCTTACGACTACTGCCAAACCTATGAGTGCAAATCTTAACCCTGAAGCCGAATTCGCGTATGGTGCTGGCCTAATAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATACGATGCTGCTGAAATCGATTACGTAAATTTTTTGTGTGCACATGGCTATAGTACCAGATTATTGAAAGCCCTTACCGGGGATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTCAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCATTCTAAAGTTCACATCTCTCGGGCAGAAGCTATCGTTTCAAGTCACGGTGAAAGGGTTGATTGAAAAAACCATAGTCTCTGGTTCTTTGGTGTGGGATGATGGTAATTTCCAAGTGAGGAGCCCCATTGTAGTGTATTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

712

Amino Acids

75.87

Weight (kDa)

6.74

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 3 - 83 1.7e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 106 - 555 1.1e-41 Subtilase family
fn3_6 PF17766 612 - 707 2e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1585
AatII GACGTC 1 cut(s) 507
Acc36I ACCTGC 2 cut(s) 1034, 1585
AccB1I GGYRCC 2 cut(s) 622, 669
AccB7I CCANNNNNTGG 1 cut(s) 402
AccI GTMKAC 1 cut(s) 1704
AccII CGCG 2 cut(s) 830, 1652
AciI CCGC 5 cut(s) 472, 641, 692, 828, 1532
AclWI GGATC 2 cut(s) 1202, 1924
AcsI RAATTY 5 cut(s) 345, 954, 998, 1646, 1732
AcuI CTGAAG 2 cut(s) 352, 1659
AcyI GRCGYC 2 cut(s) 375, 504
AdeI CACNNNGTG 1 cut(s) 1826
AfaI GTAC 4 cut(s) 647, 1312, 1344, 1759
AfiI CCNNNNNNNGG 7 cut(s) 327, 399, 402, 1457, 1694, 1782, 1783
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 6 cut(s) 167, 1064, 1280, 1411, 1693, 1957
AloI GAACNNNNNNTCC 2 cut(s) 1859, 1891
Alw21I GWGCWC 3 cut(s) 463, 1747, 1954
Alw26I GTCTC 6 cut(s) 513, 516, 742, 891, 1017, 2068
Alw44I GTGCAC 2 cut(s) 1743, 1950
AlwI GGATC 2 cut(s) 1202, 1924
Ama87I CYCGRG 2 cut(s) 1501, 2006
AoxI GGCC 7 cut(s) 350, 529, 748, 1130, 1332, 1436, 1663
ApaLI GTGCAC 2 cut(s) 1743, 1950
ApoI RAATTY 5 cut(s) 345, 954, 998, 1646, 1732
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 4 cut(s) 115, 305, 413, 1163
AspS9I GGNCC 7 cut(s) 386, 748, 848, 1130, 1333, 1364, 1899
AsuC2I CCSGG 3 cut(s) 379, 674, 1783
AsuHPI GGTGA 7 cut(s) 35, 304, 1481, 1553, 1566, 1911, 2047
AsuNHI GCTAGC 1 cut(s) 902
AvaI CYCGRG 2 cut(s) 1501, 2006
AvaII GGWCC 4 cut(s) 386, 848, 1364, 1899
AxyI CCTNAGG 1 cut(s) 39
BaeGI GKGCMC 3 cut(s) 674, 1747, 1954
BaeI ACNNNNGTAYC 4 cut(s) 309, 342, 918, 951
BanI GGYRCC 2 cut(s) 622, 669
BanII GRGCYC 2 cut(s) 1687, 2111
Bbv12I GWGCWC 3 cut(s) 463, 1747, 1954
BccI CCATC 5 cut(s) 203, 634, 1121, 1136, 2081
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 6 cut(s) 169, 1066, 1282, 1413, 1695, 1959
BciVI GTATCC 1 cut(s) 1701
BclI TGATCA 1 cut(s) 1828
BcnI CCSGG 3 cut(s) 379, 674, 1783
BcoDI GTCTC 6 cut(s) 513, 516, 742, 891, 1017, 2068
BfaI CTAG 6 cut(s) 231, 689, 903, 1427, 1677, 1809
BfmI CTRYAG 2 cut(s) 894, 1753
BfoI RGCGCY 1 cut(s) 306
BfuAI ACCTGC 2 cut(s) 1034, 1585
BfuI GTATCC 1 cut(s) 1701
BglI GCCNNNNNGGC 1 cut(s) 1080
BmcAI AGTACT 1 cut(s) 1312
Bme1390I CCNGG 9 cut(s) 169, 379, 674, 1066, 1282, 1413, 1695, 1783, 1959
Bme18I GGWCC 4 cut(s) 386, 848, 1364, 1899
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 2 cut(s) 1501, 2006
BmgT120I GGNCC 7 cut(s) 386, 748, 848, 1130, 1333, 1364, 1899
BmrFI CCNGG 9 cut(s) 169, 379, 674, 1066, 1282, 1413, 1695, 1783, 1959
BmrI ACTGGG 2 cut(s) 1146, 1445
BmsI GCATC 4 cut(s) 557, 694, 1315, 1699
BmtI GCTAGC 1 cut(s) 906
BmuI ACTGGG 2 cut(s) 1146, 1445
BpmI CTGGAG 2 cut(s) 1041, 1434
BpuEI CTTGAG 2 cut(s) 974, 1376
BpuMI CCSGG 3 cut(s) 379, 674, 1783
Bsa29I ATCGAT 2 cut(s) 1206, 1722
BsaAI YACGTR 1 cut(s) 1729
BsaHI GRCGYC 2 cut(s) 375, 504
BsaI GGTCTC 1 cut(s) 891
BsaJI CCNNGG 5 cut(s) 532, 742, 881, 930, 1782
Bsc4I CCNNNNNNNGG 7 cut(s) 327, 399, 402, 1457, 1694, 1782, 1783
Bse1I ACTGG 3 cut(s) 1141, 1335, 1451
Bse21I CCTNAGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 6 cut(s) 169, 1066, 1282, 1413, 1695, 1959
BseCI ATCGAT 2 cut(s) 1206, 1722
BseDI CCNNGG 5 cut(s) 532, 742, 881, 930, 1782
BseGI GGATG 6 cut(s) 214, 336, 1061, 1147, 1330, 2089
BseLI CCNNNNNNNGG 7 cut(s) 327, 399, 402, 1457, 1694, 1782, 1783
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 3 cut(s) 30, 1305, 1837
BseNI ACTGG 3 cut(s) 1141, 1335, 1451
BseRI GAGGAG 2 cut(s) 630, 2119
BseSI GKGCMC 3 cut(s) 674, 1747, 1954
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 2 cut(s) 830, 1652
BshFI GGCC 7 cut(s) 352, 531, 750, 1132, 1334, 1438, 1665
BshNI GGYRCC 2 cut(s) 622, 669
BshVI ATCGAT 2 cut(s) 1206, 1722
BsiHKAI GWGCWC 3 cut(s) 463, 1747, 1954
BsiHKCI CYCGRG 2 cut(s) 1501, 2006
BsiSI CCGG 3 cut(s) 378, 674, 1782
BslFI GGGAC 3 cut(s) 281, 619, 1519
BslI CCNNNNNNNGG 7 cut(s) 327, 399, 402, 1457, 1694, 1782, 1783
BsmAI GTCTC 6 cut(s) 513, 516, 742, 891, 1017, 2068
BsmBI CGTCTC 1 cut(s) 1017
BsmFI GGGAC 3 cut(s) 281, 619, 1519
BsmI GAATGC 2 cut(s) 695, 1986
BsnI GGCC 7 cut(s) 352, 531, 750, 1132, 1334, 1438, 1665
Bso31I GGTCTC 1 cut(s) 891
BsoBI CYCGRG 2 cut(s) 1501, 2006
Bsp1286I GDGCHC 6 cut(s) 463, 674, 1687, 1747, 1954, 2111
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 4 cut(s) 913, 1207, 1828, 1916
Bsp19I CCATGG 2 cut(s) 532, 881
BspACI CCGC 5 cut(s) 472, 641, 692, 828, 1532
BspANI GGCC 7 cut(s) 352, 531, 750, 1132, 1334, 1438, 1665
BspCNI CTCAG 3 cut(s) 31, 1306, 1836
BspDI ATCGAT 2 cut(s) 1206, 1722
BspFNI CGCG 2 cut(s) 830, 1652
BspMI ACCTGC 2 cut(s) 1034, 1585
BspOI GCTAGC 1 cut(s) 906
BspPI GGATC 2 cut(s) 1202, 1924
BspQI GCTCTTC 1 cut(s) 72
BspT107I GGYRCC 2 cut(s) 622, 669
BspTNI GGTCTC 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 636
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 3 cut(s) 1141, 1335, 1451
BssECI CCNNGG 5 cut(s) 532, 742, 881, 930, 1782
BssMI GATC 4 cut(s) 913, 1207, 1828, 1916
BssNAI GTATAC 1 cut(s) 1705
BssNI GRCGYC 2 cut(s) 375, 504
BssT1I CCWWGG 4 cut(s) 532, 742, 881, 930
Bst1107I GTATAC 1 cut(s) 1705
Bst2UI CCWGG 6 cut(s) 169, 1066, 1282, 1413, 1695, 1959
Bst4CI ACNGT 4 cut(s) 719, 759, 1903, 2035
Bst6I CTCTTC 2 cut(s) 72, 290
BstACI GRCGYC 2 cut(s) 375, 504
BstAPI GCANNNNNTGC 2 cut(s) 638, 1154
BstAUI TGTACA 1 cut(s) 645
BstBAI YACGTR 1 cut(s) 1729
BstC8I GCNNGC 8 cut(s) 364, 415, 634, 904, 1159, 1519, 1534, 1663
BstDEI CTNAG 5 cut(s) 39, 240, 568, 1314, 1823
BstDSI CCRYGG 2 cut(s) 532, 881
BstEII GGTNACC 1 cut(s) 1572
BstF5I GGATG 6 cut(s) 214, 336, 1061, 1147, 1330, 2089
BstFNI CGCG 2 cut(s) 830, 1652
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 4 cut(s) 115, 305, 413, 1163
BstKTI GATC 4 cut(s) 916, 1210, 1831, 1919
BstMAI GTCTC 6 cut(s) 513, 516, 742, 891, 1017, 2068
BstMBI GATC 4 cut(s) 913, 1207, 1828, 1916
BstNI CCWGG 6 cut(s) 169, 1066, 1282, 1413, 1695, 1959
BstNSI RCATGY 4 cut(s) 82, 417, 1521, 1529
BstPI GGTNACC 1 cut(s) 1572
BstSCI CCNGG 9 cut(s) 167, 377, 672, 1064, 1280, 1411, 1693, 1781, 1957
BstSFI CTRYAG 2 cut(s) 894, 1753
BstSLI GKGCMC 3 cut(s) 674, 1747, 1954
BstSNI TACGTA 1 cut(s) 1729
BstUI CGCG 2 cut(s) 830, 1652
BstZ17I GTATAC 1 cut(s) 1705
Bsu15I ATCGAT 2 cut(s) 1206, 1722
Bsu36I CCTNAGG 1 cut(s) 39
BsuI GTATCC 1 cut(s) 1701
BsuRI GGCC 7 cut(s) 352, 531, 750, 1132, 1334, 1438, 1665
BsuTUI ATCGAT 2 cut(s) 1206, 1722
BtgI CCRYGG 2 cut(s) 532, 881
BtgZI GCGATG 1 cut(s) 1140
BtsCI GGATG 6 cut(s) 214, 336, 1061, 1147, 1330, 2089
BtsI GCAGTG 2 cut(s) 505, 543
BtsIMutI CAGTG 5 cut(s) 376, 505, 543, 1328, 1831
BveI ACCTGC 2 cut(s) 1034, 1585
Cac8I GCNNGC 8 cut(s) 364, 415, 634, 904, 1159, 1519, 1534, 1663
CfoI GCGC 4 cut(s) 115, 305, 413, 1163
Cfr13I GGNCC 7 cut(s) 386, 748, 848, 1130, 1333, 1364, 1899
ClaI ATCGAT 2 cut(s) 1206, 1722
CseI GACGC 3 cut(s) 383, 686, 819
Csp6I GTAC 4 cut(s) 646, 1311, 1343, 1758
CspCI CAANNNNNGTGG 2 cut(s) 1083, 1118
CviQI GTAC 4 cut(s) 646, 1311, 1343, 1758
DdeI CTNAG 5 cut(s) 39, 240, 568, 1314, 1823
DpnI GATC 4 cut(s) 915, 1209, 1830, 1918
DpnII GATC 4 cut(s) 913, 1207, 1828, 1916
DraIII CACNNNGTG 1 cut(s) 1826
DriI GACNNNNNGTC 1 cut(s) 508
Eam1104I CTCTTC 2 cut(s) 72, 290
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 2 cut(s) 72, 290
Eco105I TACGTA 1 cut(s) 1729
Eco130I CCWWGG 4 cut(s) 532, 742, 881, 930
Eco24I GRGCYC 2 cut(s) 1687, 2111
Eco31I GGTCTC 1 cut(s) 891
Eco32I GATATC 1 cut(s) 1402
Eco47I GGWCC 4 cut(s) 386, 848, 1364, 1899
Eco57I CTGAAG 2 cut(s) 352, 1659
Eco81I CCTNAGG 1 cut(s) 39
Eco88I CYCGRG 2 cut(s) 1501, 2006
Eco91I GGTNACC 1 cut(s) 1572
EcoO65I GGTNACC 1 cut(s) 1572
EcoRI GAATTC 1 cut(s) 1646
EcoRII CCWGG 6 cut(s) 167, 1064, 1280, 1411, 1693, 1957
EcoRV GATATC 1 cut(s) 1402
EcoT14I CCWWGG 4 cut(s) 532, 742, 881, 930
EcoT38I GRGCYC 2 cut(s) 1687, 2111
ErhI CCWWGG 4 cut(s) 532, 742, 881, 930
Esp3I CGTCTC 1 cut(s) 1017
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 3 cut(s) 281, 619, 1519
FauI CCCGC 1 cut(s) 1525
FauNDI CATATG 1 cut(s) 1339
FbaI TGATCA 1 cut(s) 1828
FblI GTMKAC 1 cut(s) 1704
FokI GGATG 6 cut(s) 221, 343, 1048, 1154, 1337, 2096
FriOI GRGCYC 2 cut(s) 1687, 2111
FspBI CTAG 6 cut(s) 231, 689, 903, 1427, 1677, 1809
GlaI GCGC 4 cut(s) 114, 304, 412, 1162
GsuI CTGGAG 2 cut(s) 1041, 1434
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 7 cut(s) 352, 531, 750, 1132, 1334, 1438, 1665
HapII CCGG 3 cut(s) 378, 674, 1782
HgaI GACGC 3 cut(s) 383, 686, 819
HhaI GCGC 4 cut(s) 115, 305, 413, 1163
Hin1I GRCGYC 2 cut(s) 375, 504
Hin6I GCGC 4 cut(s) 113, 303, 411, 1161
HinP1I GCGC 4 cut(s) 113, 303, 411, 1161
HincII GTYRAC 2 cut(s) 479, 724
HindII GTYRAC 2 cut(s) 479, 724
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 3 cut(s) 119, 338, 356
HpaII CCGG 3 cut(s) 378, 674, 1782
HphI GGTGA 7 cut(s) 35, 304, 1481, 1553, 1566, 1911, 2047
Hpy188I TCNGA 4 cut(s) 458, 516, 664, 773
Hpy188III TCNNGA 4 cut(s) 284, 328, 342, 1031
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 6 cut(s) 419, 521, 839, 1356, 1460, 1854
HpyCH4III ACNGT 4 cut(s) 719, 759, 1903, 2035
HpyCH4IV ACGT 6 cut(s) 90, 504, 1026, 1085, 1548, 1728
HpyF3I CTNAG 5 cut(s) 39, 240, 568, 1314, 1823
HpySE526I ACGT 6 cut(s) 90, 504, 1026, 1085, 1548, 1728
Hsp92I GRCGYC 2 cut(s) 375, 504
HspAI GCGC 4 cut(s) 113, 303, 411, 1161
Ksp22I TGATCA 1 cut(s) 1828
Kzo9I GATC 4 cut(s) 913, 1207, 1828, 1916
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 5 cut(s) 252, 458, 791, 1690, 2106
LweI GCATC 4 cut(s) 557, 694, 1315, 1699
MaeI CTAG 6 cut(s) 231, 689, 903, 1427, 1677, 1809
MaeII ACGT 6 cut(s) 90, 504, 1026, 1085, 1548, 1728
MaeIII GTNAC 9 cut(s) 23, 371, 500, 970, 1046, 1250, 1572, 1903, 2029
MalI GATC 4 cut(s) 915, 1209, 1830, 1918
MboI GATC 4 cut(s) 913, 1207, 1828, 1916
MboII GAAGA 3 cut(s) 59, 194, 307
MfeI CAATTG 3 cut(s) 938, 1443, 1567
MhlI GDGCHC 6 cut(s) 463, 674, 1687, 1747, 1954, 2111
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 1894
MseI TTAA 3 cut(s) 806, 1632, 1977
MslI CAYNNNNRTG 2 cut(s) 641, 2117
MspI CCGG 3 cut(s) 378, 674, 1782
MspR9I CCNGG 9 cut(s) 169, 379, 674, 1066, 1282, 1413, 1695, 1783, 1959
MunI CAATTG 3 cut(s) 938, 1443, 1567
Mva1269I GAATGC 2 cut(s) 695, 1986
MvaI CCWGG 6 cut(s) 169, 1066, 1282, 1413, 1695, 1959
MvnI CGCG 2 cut(s) 830, 1652
NciI CCSGG 3 cut(s) 379, 674, 1783
NcoI CCATGG 2 cut(s) 532, 881
NdeI CATATG 1 cut(s) 1339
NdeII GATC 4 cut(s) 913, 1207, 1828, 1916
NheI GCTAGC 1 cut(s) 902
NmuCI GTSAC 6 cut(s) 23, 371, 500, 1572, 1903, 2029
NspI RCATGY 4 cut(s) 82, 417, 1521, 1529
PaeI GCATGC 2 cut(s) 417, 1521
PaqCI CACCTGC 1 cut(s) 1585
PciSI GCTCTTC 1 cut(s) 72
PctI GAATGC 2 cut(s) 695, 1986
PfeI GAWTC 2 cut(s) 119, 356
PflMI CCANNNNNTGG 1 cut(s) 402
PfoI TCCNGGA 1 cut(s) 1411
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 1729
Psp6I CCWGG 6 cut(s) 167, 1064, 1280, 1411, 1693, 1957
PspEI GGTNACC 1 cut(s) 1572
PspGI CCWGG 6 cut(s) 167, 1064, 1280, 1411, 1693, 1957
PspPI GGNCC 7 cut(s) 386, 748, 848, 1130, 1333, 1364, 1899
RsaI GTAC 4 cut(s) 647, 1312, 1344, 1759
RsaNI GTAC 4 cut(s) 646, 1311, 1343, 1758
RseI CAYNNNNRTG 2 cut(s) 641, 2117
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 806, 1632, 1977
Sau3AI GATC 4 cut(s) 913, 1207, 1828, 1916
Sau96I GGNCC 7 cut(s) 386, 748, 848, 1130, 1333, 1364, 1899
ScaI AGTACT 1 cut(s) 1312
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 9 cut(s) 169, 379, 674, 1066, 1282, 1413, 1695, 1783, 1959
SduI GDGCHC 6 cut(s) 463, 674, 1687, 1747, 1954, 2111
SfaNI GCATC 4 cut(s) 557, 694, 1315, 1699
SfcI CTRYAG 2 cut(s) 894, 1753
SinI GGWCC 4 cut(s) 386, 848, 1364, 1899
SmiMI CAYNNNNRTG 2 cut(s) 641, 2117
SmlI CTYRAG 2 cut(s) 989, 1391
SmoI CTYRAG 2 cut(s) 989, 1391
SnaBI TACGTA 1 cut(s) 1729
SphI GCATGC 2 cut(s) 417, 1521
SsiI CCGC 5 cut(s) 472, 641, 692, 828, 1532
SspI AATATT 2 cut(s) 819, 1387
SspMI CTAG 6 cut(s) 231, 689, 903, 1427, 1677, 1809
StyD4I CCNGG 9 cut(s) 167, 377, 672, 1064, 1280, 1411, 1693, 1781, 1957
StyI CCWWGG 4 cut(s) 532, 742, 881, 930
TaaI ACNGT 4 cut(s) 719, 759, 1903, 2035
TaiI ACGT 6 cut(s) 93, 507, 1029, 1088, 1551, 1731
TaqI TCGA 5 cut(s) 96, 699, 1206, 1722, 1865
TatI WGTACW 2 cut(s) 645, 1310
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 2 cut(s) 119, 356
Tru1I TTAA 3 cut(s) 806, 1632, 1977
Tru9I TTAA 3 cut(s) 806, 1632, 1977
TscAI CASTG 5 cut(s) 376, 505, 550, 1335, 1831
TseFI GTSAC 6 cut(s) 23, 371, 500, 1572, 1903, 2029
Tsp45I GTSAC 6 cut(s) 23, 371, 500, 1572, 1903, 2029
TspDTI ATGAA 6 cut(s) 59, 132, 262, 858, 1338, 1476
TspGWI ACGGA 4 cut(s) 539, 1222, 1677, 1867
TspRI CASTG 5 cut(s) 376, 505, 550, 1335, 1831
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 2 cut(s) 1743, 1950
VpaK11BI GGWCC 4 cut(s) 386, 848, 1364, 1899
XapI RAATTY 5 cut(s) 345, 954, 998, 1646, 1732
XceI RCATGY 4 cut(s) 82, 417, 1521, 1529
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 1704
XspI CTAG 6 cut(s) 231, 689, 903, 1427, 1677, 1809
ZraI GACGTC 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.