Rroxscaffold_4G00287250

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Reverse (-)
8287193 .. 8291007
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287250.1

Sequence Viewer

Length: 2181 bp
ATGAGAGCAATGCCTCTAGCTAGTAGCACCATACAGAGTAATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAGTATTCTACTTCTTGTCGATGTTACTCATTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAATGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATGTTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGAAACAGTTAAGAGAGGCGTCCTTGAAAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATACTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAATATTATCGTGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGCTCAAGGCAAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATATTCTAGCCGCATTTGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCGTCGGGGGCAATATTCCACTTGATTATTTGAATGATTCATTCGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAATGAAGGTCCGGGCGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAATCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCGCCCAATACAAAAGCAGGTGCTGACGGGAGTATCTCTAGGTTTTGTGTACGAGATGCCTTAGAAGAAGATTTGATCAAAGGTAAAATTGTGTTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCCATATTGGGTGGTGCAGCCGGAATTGTTCTGACGGGAAAAAAAGTCGACGATGAACTGTTTAGCCTTTATCCCGTGCCTGCATCTTTCGTTGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAATTCAACAAGGAACGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATACGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCTGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGCGGTGGTGGGGGTAGAGGCAGATACTATATGGACACCGGGACATCAATGGCATGCCCCCATGCTACGGCCACAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCAGCGGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAATGCCACAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAGGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATCTGGTCTCTGCATCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

726

Amino Acids

77.37

Weight (kDa)

7.11

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 48 - 111 1e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 122 - 569 5e-43 Subtilase family
fn3_6 PF17766 625 - 722 7.5e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1085
Acc36I ACCTGC 1 cut(s) 1085
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 3 cut(s) 178, 667, 1726
AccB7I CCANNNNNTGG 4 cut(s) 173, 453, 1511, 1736
AccI GTMKAC 2 cut(s) 774, 1254
AccII CGCG 2 cut(s) 679, 1079
AciI CCGC 6 cut(s) 159, 591, 744, 885, 1509, 1619
AclWI GGATC 3 cut(s) 1904, 1917, 1966
AcoI YGGCCR 3 cut(s) 401, 882, 1575
AcsI RAATTY 5 cut(s) 396, 1049, 1336, 1688, 1916
AcyI GRCGYC 2 cut(s) 354, 1727
AfaI GTAC 3 cut(s) 180, 692, 1128
AgsI TTSAA 7 cut(s) 289, 338, 362, 541, 823, 989, 1341
AhlI ACTAGT 1 cut(s) 1835
AjnI CCWGG 3 cut(s) 1474, 1735, 1823
Alw21I GWGCWC 2 cut(s) 514, 621
Alw26I GTCTC 4 cut(s) 558, 561, 1068, 2113
AlwI GGATC 3 cut(s) 1904, 1917, 1966
AlwNI CAGNNNCTG 3 cut(s) 605, 1100, 1622
Ama87I CYCGRG 1 cut(s) 2048
AoxI GGCC 8 cut(s) 401, 882, 1180, 1206, 1426, 1575, 1705, 2084
ApaI GGGCCC 1 cut(s) 1430
ApoI RAATTY 5 cut(s) 396, 1049, 1336, 1688, 1916
ArsI GACNNNNNNTTYG 2 cut(s) 1673, 1705
AseI ATTAAT 1 cut(s) 1857
Asp718I GGTACC 1 cut(s) 178
AspLEI GCGC 4 cut(s) 679, 908, 1081, 1729
AspS9I GGNCC 7 cut(s) 437, 899, 1181, 1206, 1426, 1427, 2084
AsuC2I CCSGG 3 cut(s) 903, 1043, 1546
AsuHPI GGTGA 2 cut(s) 970, 1595
AvaI CYCGRG 1 cut(s) 2048
AvaII GGWCC 2 cut(s) 437, 899
BaeGI GKGCMC 2 cut(s) 1410, 1430
BaeI ACNNNNGTAYC 4 cut(s) 1093, 1126, 1786, 1819
BalI TGGCCA 1 cut(s) 403
BamHI GGATCC 1 cut(s) 1909
BanI GGYRCC 3 cut(s) 178, 667, 1726
BanII GRGCYC 2 cut(s) 1430, 2156
BbsI GAAGAC 1 cut(s) 1924
Bbv12I GWGCWC 2 cut(s) 514, 621
BccI CCATC 5 cut(s) 51, 679, 1172, 1910, 2126
BceAI ACGGC 1 cut(s) 1590
BciT130I CCWGG 3 cut(s) 1476, 1737, 1825
BciVI GTATCC 1 cut(s) 1787
BclI TGATCA 1 cut(s) 1152
BcnI CCSGG 3 cut(s) 903, 1043, 1546
BcoDI GTCTC 4 cut(s) 558, 561, 1068, 2113
BcuI ACTAGT 1 cut(s) 1835
BfaI CTAG 7 cut(s) 17, 21, 740, 1116, 1674, 1722, 1836
BfoI RGCGCY 1 cut(s) 1730
BfuAI ACCTGC 1 cut(s) 1085
BfuI GTATCC 1 cut(s) 1787
BglI GCCNNNNNGGC 1 cut(s) 137
BglII AGATCT 1 cut(s) 256
Bme1390I CCNGG 6 cut(s) 903, 1043, 1476, 1546, 1737, 1825
Bme18I GGWCC 2 cut(s) 437, 899
BmeT110I CYCGRG 1 cut(s) 2048
BmgT120I GGNCC 7 cut(s) 437, 899, 1181, 1206, 1426, 1427, 2084
BmrFI CCNGG 6 cut(s) 903, 1043, 1476, 1546, 1737, 1825
BmsI GCATC 7 cut(s) 118, 602, 718, 745, 1123, 1298, 2123
BpiI GAAGAC 1 cut(s) 1924
BpmI CTGGAG 1 cut(s) 1458
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 2 cut(s) 605, 1439
BpuMI CCSGG 3 cut(s) 903, 1043, 1546
BsaAI YACGTR 1 cut(s) 1405
BsaHI GRCGYC 2 cut(s) 354, 1727
BsaI GGTCTC 1 cut(s) 2113
BsaJI CCNNGG 5 cut(s) 879, 932, 981, 1042, 1824
BsaXI ACNNNNNCTCC 4 cut(s) 1394, 1424, 1978, 2008
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 4 cut(s) 15, 309, 481, 759
BseBI CCWGG 3 cut(s) 1476, 1737, 1825
BseDI CCNNGG 5 cut(s) 879, 932, 981, 1042, 1824
BseGI GGATG 3 cut(s) 133, 1195, 2134
BseMI GCAATG 4 cut(s) 15, 309, 481, 759
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 2 cut(s) 1410, 1430
BseYI CCCAGC 2 cut(s) 605, 1196
BsgI GTGCAG 1 cut(s) 1242
Bsh1236I CGCG 2 cut(s) 679, 1079
BshFI GGCC 8 cut(s) 403, 884, 1182, 1208, 1428, 1577, 1707, 2086
BshNI GGYRCC 3 cut(s) 178, 667, 1726
BsiHKAI GWGCWC 2 cut(s) 514, 621
BsiHKCI CYCGRG 1 cut(s) 2048
BsiSI CCGG 4 cut(s) 902, 1042, 1227, 1545
BslFI GGGAC 5 cut(s) 423, 1029, 1561, 1841, 2016
BsmAI GTCTC 4 cut(s) 558, 561, 1068, 2113
BsmBI CGTCTC 1 cut(s) 1068
BsmFI GGGAC 5 cut(s) 423, 1029, 1561, 1841, 2016
BsmI GAATGC 1 cut(s) 1669
BsnI GGCC 8 cut(s) 403, 884, 1182, 1208, 1428, 1577, 1707, 2086
Bso31I GGTCTC 1 cut(s) 2113
BsoBI CYCGRG 1 cut(s) 2048
Bsp120I GGGCCC 1 cut(s) 1426
Bsp1286I GDGCHC 5 cut(s) 514, 621, 1410, 1430, 2156
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
Bsp19I CCATGG 2 cut(s) 879, 932
BspACI CCGC 6 cut(s) 159, 591, 744, 885, 1509, 1619
BspANI GGCC 8 cut(s) 403, 884, 1182, 1208, 1428, 1577, 1707, 2086
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 2 cut(s) 679, 1079
BspMI ACCTGC 1 cut(s) 1085
BspPI GGATC 3 cut(s) 1904, 1917, 1966
BspQI GCTCTTC 1 cut(s) 57
BspT107I GGYRCC 3 cut(s) 178, 667, 1726
BspTNI GGTCTC 1 cut(s) 2113
BsrDI GCAATG 4 cut(s) 15, 309, 481, 759
BsrGI TGTACA 1 cut(s) 690
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 5 cut(s) 879, 932, 981, 1042, 1824
BssMI GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
BssNI GRCGYC 2 cut(s) 354, 1727
BssT1I CCWWGG 3 cut(s) 879, 932, 981
Bst2UI CCWGG 3 cut(s) 1476, 1737, 1825
Bst6I CTCTTC 2 cut(s) 57, 1371
BstACI GRCGYC 2 cut(s) 354, 1727
BstAUI TGTACA 1 cut(s) 690
BstBAI YACGTR 1 cut(s) 1405
BstC8I GCNNGC 6 cut(s) 164, 628, 1287, 1561, 1705, 1815
BstDEI CTNAG 2 cut(s) 123, 1138
BstDSI CCRYGG 2 cut(s) 879, 932
BstF5I GGATG 3 cut(s) 133, 1195, 2134
BstFNI CGCG 2 cut(s) 679, 1079
BstH2I RGCGCY 1 cut(s) 1730
BstHHI GCGC 4 cut(s) 679, 908, 1081, 1729
BstKTI GATC 7 cut(s) 196, 259, 1155, 1440, 1753, 1912, 1961
BstMAI GTCTC 4 cut(s) 558, 561, 1068, 2113
BstMBI GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
BstNI CCWGG 3 cut(s) 1476, 1737, 1825
BstNSI RCATGY 2 cut(s) 702, 1563
BstSCI CCNGG 6 cut(s) 901, 1041, 1474, 1544, 1735, 1823
BstSLI GKGCMC 2 cut(s) 1410, 1430
BstUI CGCG 2 cut(s) 679, 1079
BstV2I GAAGAC 1 cut(s) 1924
BstX2I RGATCY 2 cut(s) 256, 1909
BstYI RGATCY 2 cut(s) 256, 1909
BsuI GTATCC 1 cut(s) 1787
BsuRI GGCC 8 cut(s) 403, 884, 1182, 1208, 1428, 1577, 1707, 2086
BtgI CCRYGG 2 cut(s) 879, 932
BtsCI GGATG 3 cut(s) 133, 1195, 2134
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 4 cut(s) 397, 621, 718, 1804
BveI ACCTGC 1 cut(s) 1085
Cac8I GCNNGC 6 cut(s) 164, 628, 1287, 1561, 1705, 1815
CaiI CAGNNNCTG 3 cut(s) 605, 1100, 1622
CfoI GCGC 4 cut(s) 679, 908, 1081, 1729
Cfr13I GGNCC 7 cut(s) 437, 899, 1181, 1206, 1426, 1427, 2084
CseI GACGC 3 cut(s) 343, 1085, 1691
Csp6I GTAC 3 cut(s) 179, 691, 1127
CviAII CATG 9 cut(s) 200, 251, 578, 699, 880, 933, 1560, 1568, 1876
CviQI GTAC 3 cut(s) 179, 691, 1127
DdeI CTNAG 2 cut(s) 123, 1138
DinI GGCGCC 1 cut(s) 1728
DpnI GATC 7 cut(s) 195, 258, 1154, 1439, 1752, 1911, 1960
DpnII GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
EaeI YGGCCR 3 cut(s) 401, 882, 1575
Eam1104I CTCTTC 2 cut(s) 57, 1371
EarI CTCTTC 2 cut(s) 57, 1371
Eco130I CCWWGG 3 cut(s) 879, 932, 981
Eco24I GRGCYC 2 cut(s) 1430, 2156
Eco31I GGTCTC 1 cut(s) 2113
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 2 cut(s) 437, 899
Eco88I CYCGRG 1 cut(s) 2048
EcoO109I RGGNCCY 1 cut(s) 1426
EcoRII CCWGG 3 cut(s) 1474, 1735, 1823
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 3 cut(s) 879, 932, 981
EcoT38I GRGCYC 2 cut(s) 1430, 2156
EgeI GGCGCC 1 cut(s) 1728
EheI GGCGCC 1 cut(s) 1728
ErhI CCWWGG 3 cut(s) 879, 932, 981
Esp3I CGTCTC 1 cut(s) 1068
FaeI CATG 9 cut(s) 203, 254, 581, 702, 883, 936, 1563, 1571, 1879
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 614, 646
FaqI GGGAC 5 cut(s) 423, 1029, 1561, 1841, 2016
FatI CATG 9 cut(s) 199, 250, 577, 698, 879, 932, 1559, 1567, 1875
FbaI TGATCA 1 cut(s) 1152
FblI GTMKAC 2 cut(s) 774, 1254
FokI GGATG 3 cut(s) 140, 1202, 2141
FriOI GRGCYC 2 cut(s) 1430, 2156
FspBI CTAG 7 cut(s) 17, 21, 740, 1116, 1674, 1722, 1836
GlaI GCGC 4 cut(s) 678, 907, 1080, 1728
GsaI CCCAGC 2 cut(s) 609, 1200
GsuI CTGGAG 1 cut(s) 1458
HaeII RGCGCY 1 cut(s) 1730
HaeIII GGCC 8 cut(s) 403, 884, 1182, 1208, 1428, 1577, 1707, 2086
HapII CCGG 4 cut(s) 902, 1042, 1227, 1545
HgaI GACGC 3 cut(s) 343, 1085, 1691
HhaI GCGC 4 cut(s) 679, 908, 1081, 1729
Hin1I GRCGYC 2 cut(s) 354, 1727
Hin1II CATG 9 cut(s) 203, 254, 581, 702, 883, 936, 1563, 1571, 1879
Hin6I GCGC 4 cut(s) 677, 906, 1079, 1727
HinP1I GCGC 4 cut(s) 677, 906, 1079, 1727
HincII GTYRAC 2 cut(s) 226, 1255
HindII GTYRAC 2 cut(s) 226, 1255
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 6 cut(s) 407, 554, 566, 827, 2004, 2020
HpaII CCGG 4 cut(s) 902, 1042, 1227, 1545
HphI GGTGA 2 cut(s) 970, 1595
Hpy166II GTNNAC 8 cut(s) 226, 691, 775, 1127, 1255, 1787, 1805, 2040
Hpy188I TCNGA 5 cut(s) 412, 509, 1239, 1437, 1914
Hpy188III TCNNGA 4 cut(s) 125, 648, 1754, 2149
Hpy8I GTNNAC 8 cut(s) 226, 691, 775, 1127, 1255, 1787, 1805, 2040
Hpy99I CGWCG 2 cut(s) 797, 1259
HpyAV CCTTC 4 cut(s) 77, 890, 1728, 1896
HpyCH4IV ACGT 3 cut(s) 219, 1404, 1590
HpyF3I CTNAG 2 cut(s) 123, 1138
HpySE526I ACGT 3 cut(s) 219, 1404, 1590
Hsp92I GRCGYC 2 cut(s) 354, 1727
Hsp92II CATG 9 cut(s) 203, 254, 581, 702, 883, 936, 1563, 1571, 1879
HspAI GCGC 4 cut(s) 677, 906, 1079, 1727
KasI GGCGCC 1 cut(s) 1726
KpnI GGTACC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 1152
Kzo9I GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
LguI GCTCTTC 1 cut(s) 57
LmnI GCTCC 6 cut(s) 509, 1402, 1477, 1700, 1999, 2151
LweI GCATC 7 cut(s) 118, 602, 718, 745, 1123, 1298, 2123
MaeI CTAG 7 cut(s) 17, 21, 740, 1116, 1674, 1722, 1836
MaeII ACGT 3 cut(s) 219, 1404, 1590
MaeIII GTNAC 7 cut(s) 106, 491, 722, 916, 995, 1945, 2071
MalI GATC 7 cut(s) 195, 258, 1154, 1439, 1752, 1911, 1960
MboI GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
MfeI CAATTG 2 cut(s) 837, 989
MflI RGATCY 2 cut(s) 256, 1909
MhlI GDGCHC 5 cut(s) 514, 621, 1410, 1430, 2156
MlsI TGGCCA 1 cut(s) 403
MluNI TGGCCA 1 cut(s) 403
Mly113I GGCGCC 1 cut(s) 1727
MlyI GAGTC 4 cut(s) 416, 560, 563, 1998
MmeI TCCRAC 3 cut(s) 390, 1284, 1936
Mox20I TGGCCA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 403
MseI TTAA 5 cut(s) 345, 633, 1061, 1857, 2179
Msp20I TGGCCA 1 cut(s) 403
MspA1I CMGCKG 5 cut(s) 119, 605, 887, 1583, 1619
MspI CCGG 4 cut(s) 902, 1042, 1227, 1545
MspR9I CCNGG 6 cut(s) 903, 1043, 1476, 1546, 1737, 1825
MunI CAATTG 2 cut(s) 837, 989
Mva1269I GAATGC 1 cut(s) 1669
MvaI CCWGG 3 cut(s) 1476, 1737, 1825
MvnI CGCG 2 cut(s) 679, 1079
NarI GGCGCC 1 cut(s) 1727
NciI CCSGG 3 cut(s) 903, 1043, 1546
NcoI CCATGG 2 cut(s) 879, 932
NdeII GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
NlaIII CATG 9 cut(s) 203, 254, 581, 702, 883, 936, 1563, 1571, 1879
NmuCI GTSAC 4 cut(s) 722, 916, 1945, 2071
NspI RCATGY 2 cut(s) 702, 1563
PaeI GCATGC 1 cut(s) 1563
PaqCI CACCTGC 1 cut(s) 1085
PciSI GCTCTTC 1 cut(s) 57
PctI GAATGC 1 cut(s) 1669
PfeI GAWTC 2 cut(s) 827, 2020
PflMI CCANNNNNTGG 4 cut(s) 173, 453, 1511, 1736
PfoI TCCNGGA 1 cut(s) 1474
PleI GAGTC 4 cut(s) 415, 560, 562, 1998
PluTI GGCGCC 1 cut(s) 1730
PpsI GAGTC 4 cut(s) 415, 560, 562, 1998
Ppu21I YACGTR 1 cut(s) 1405
PshBI ATTAAT 1 cut(s) 1857
Psp6I CCWGG 3 cut(s) 1474, 1735, 1823
PspFI CCCAGC 2 cut(s) 605, 1196
PspGI CCWGG 3 cut(s) 1474, 1735, 1823
PspOMI GGGCCC 1 cut(s) 1426
PspPI GGNCC 7 cut(s) 437, 899, 1181, 1206, 1426, 1427, 2084
PstNI CAGNNNCTG 3 cut(s) 605, 1100, 1622
PsuI RGATCY 2 cut(s) 256, 1909
PvuII CAGCTG 3 cut(s) 119, 605, 1583
RsaI GTAC 3 cut(s) 180, 692, 1128
RsaNI GTAC 3 cut(s) 179, 691, 1127
SalI GTCGAC 1 cut(s) 1253
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 5 cut(s) 345, 633, 1061, 1857, 2179
Sau3AI GATC 7 cut(s) 193, 256, 1152, 1437, 1750, 1909, 1958
Sau96I GGNCC 7 cut(s) 437, 899, 1181, 1206, 1426, 1427, 2084
SchI GAGTC 4 cut(s) 416, 560, 563, 1998
ScrFI CCNGG 6 cut(s) 903, 1043, 1476, 1546, 1737, 1825
SduI GDGCHC 5 cut(s) 514, 621, 1410, 1430, 2156
SfaNI GCATC 7 cut(s) 118, 602, 718, 745, 1123, 1298, 2123
SfoI GGCGCC 1 cut(s) 1728
SinI GGWCC 2 cut(s) 437, 899
SmlI CTYRAG 2 cut(s) 620, 1454
SmoI CTYRAG 2 cut(s) 620, 1454
SpeI ACTAGT 1 cut(s) 1835
SphI GCATGC 1 cut(s) 1563
SsiI CCGC 6 cut(s) 159, 591, 744, 885, 1509, 1619
SspDI GGCGCC 1 cut(s) 1726
SspI AATATT 2 cut(s) 518, 805
SspMI CTAG 7 cut(s) 17, 21, 740, 1116, 1674, 1722, 1836
StyD4I CCNGG 6 cut(s) 901, 1041, 1474, 1544, 1735, 1823
StyI CCWWGG 3 cut(s) 879, 932, 981
TaiI ACGT 3 cut(s) 222, 1407, 1593
TaqI TCGA 4 cut(s) 55, 102, 1254, 1421
TatI WGTACW 1 cut(s) 690
TauI GCSGC 4 cut(s) 162, 746, 887, 1622
TfiI GAWTC 2 cut(s) 827, 2020
Tru1I TTAA 5 cut(s) 345, 633, 1061, 1857, 2179
Tru9I TTAA 5 cut(s) 345, 633, 1061, 1857, 2179
TscAI CASTG 4 cut(s) 397, 621, 718, 1804
TseFI GTSAC 4 cut(s) 722, 916, 1945, 2071
Tsp45I GTSAC 4 cut(s) 722, 916, 1945, 2071
TspDTI ATGAA 9 cut(s) 188, 267, 819, 909, 964, 1275, 1676, 1773, 1892
TspGWI ACGGA 2 cut(s) 781, 1713
TspRI CASTG 4 cut(s) 397, 621, 718, 1804
Van91I CCANNNNNTGG 4 cut(s) 173, 453, 1511, 1736
VpaK11BI GGWCC 2 cut(s) 437, 899
VspI ATTAAT 1 cut(s) 1857
XapI RAATTY 5 cut(s) 396, 1049, 1336, 1688, 1916
XceI RCATGY 2 cut(s) 702, 1563
XcmI CCANNNNNNNNNTGG 2 cut(s) 450, 988
XmiI GTMKAC 2 cut(s) 774, 1254
XspI CTAG 7 cut(s) 17, 21, 740, 1116, 1674, 1722, 1836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.