RchiOBHm_Chr1g0344281

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
36600062 .. 36601183
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57074

Sequence Viewer

Length: 924 bp
ATGCATGTTAATCTTGATCTTTATAGGAACCCAATTGCAACTATTTTCAAAAGTGAAGAAATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCGTCAAGGGGTCCAAATCCAATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCTCCAGGTACTCACATTCTAGCAGCATATGATCACCAACAATCAGTTGGTGATAGCAGAACTGAATATAATTTGGATAGTGGGACATCAATGGCGTGCCCTCATGCTGCAGGCGCAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCAGCTCTCATCACTACAGTTATCAGACCACGAAAGAAAACACCTAAACATTCTTGTGTATTCCAATCTTTCGCAGCTAAACCTATGAGTGCCAAAACTAGCCCGCACGCTGAATTCGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGGAAATCATAACCGGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAATAGCCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTCATCAATGGGATCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAATTCCACATACAGAGCTAAAGTGGTGACTCCATCAGGACTCGAAATCAATGTGAATCCAAATGTGTTATCATTCACATCTCTCGGCCAGAAGAAACCTTTTGTCGTTACAGTAAAAGGGTCAATTGAGAAATCAAATATAGTTTCTACATCTTTGGTGTGGGATAATGGTGATTTCCAAGTCAGGAGCCCGATTGTTGTCTATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

307

Amino Acids

33.39

Weight (kDa)

8.52

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 28 - 150 1.4e-17 Subtilase family
fn3_6 PF17766 206 - 303 2.6e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 479
AccI GTMKAC 1 cut(s) 489
AciI CCGC 1 cut(s) 422
AclWI GGATC 4 cut(s) 647, 660, 680, 709
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 3 cut(s) 431, 659, 709
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 466, 479
AgsI TTSAA 3 cut(s) 49, 317, 645
AjnI CCWGG 2 cut(s) 154, 478
AluBI AGCT 7 cut(s) 152, 269, 323, 395, 446, 582, 725
AluI AGCT 7 cut(s) 152, 269, 323, 395, 446, 582, 725
AlwI GGATC 4 cut(s) 647, 660, 680, 709
AoxI GGCC 2 cut(s) 448, 793
ApeKI GCWGC 8 cut(s) 149, 173, 257, 266, 269, 308, 392, 582
ApoI RAATTY 3 cut(s) 431, 659, 709
AseI ATTAAT 1 cut(s) 63
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 568
AsuHPI GGTGA 5 cut(s) 176, 212, 294, 745, 890
AvaII GGWCC 1 cut(s) 107
BaeGI GKGCMC 2 cut(s) 90, 251
BaeI ACNNNNGTAYC 2 cut(s) 529, 562
BamHI GGATCC 1 cut(s) 652
BanII GRGCYC 2 cut(s) 472, 899
BbvI GCAGC 8 cut(s) 161, 185, 244, 256, 278, 295, 404, 569
BccI CCATC 1 cut(s) 748
BciT130I CCWGG 2 cut(s) 156, 480
BciVI GTATCC 1 cut(s) 530
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 568
BfaI CTAG 3 cut(s) 170, 417, 465
BfmI CTRYAG 2 cut(s) 258, 333
BfuI GTATCC 1 cut(s) 530
BisI GCNGC 8 cut(s) 150, 174, 258, 267, 270, 309, 393, 583
BlsI GCNGC 8 cut(s) 151, 175, 259, 268, 271, 310, 394, 584
Bme1390I CCNGG 3 cut(s) 156, 480, 568
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 6 cut(s) 29, 78, 108, 471, 654, 896
BmrFI CCNGG 3 cut(s) 156, 480, 568
BpmI CTGGAG 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 568
BsaAI YACGTR 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 466, 479
Bse118I RCCGGY 1 cut(s) 304
BseBI CCWGG 2 cut(s) 156, 480
BseDI CCNNGG 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 466, 479
BseSI GKGCMC 2 cut(s) 90, 251
BseXI GCAGC 8 cut(s) 161, 185, 244, 256, 278, 295, 404, 569
BshFI GGCC 2 cut(s) 450, 795
BsiSI CCGG 2 cut(s) 305, 567
BslFI GGGAC 2 cut(s) 247, 584
BslI CCNNNNNNNGG 2 cut(s) 466, 479
BsmFI GGGAC 2 cut(s) 247, 584
BsnI GGCC 2 cut(s) 450, 795
Bsp1286I GDGCHC 4 cut(s) 90, 251, 472, 899
Bsp143I GATC 6 cut(s) 16, 181, 493, 652, 672, 701
BspACI CCGC 1 cut(s) 422
BspANI GGCC 2 cut(s) 450, 795
BspLI GGNNCC 6 cut(s) 29, 78, 108, 471, 654, 896
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 4 cut(s) 647, 660, 680, 709
BsrFI RCCGGY 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 6 cut(s) 16, 181, 493, 652, 672, 701
BssNAI GTATAC 1 cut(s) 490
Bst1107I GTATAC 1 cut(s) 490
Bst2UI CCWGG 2 cut(s) 156, 480
Bst4CI ACNGT 5 cut(s) 337, 542, 688, 820, 919
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 5 cut(s) 247, 262, 422, 426, 448
BstEII GGTNACC 1 cut(s) 300
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 6 cut(s) 19, 184, 496, 655, 675, 704
BstMBI GATC 6 cut(s) 16, 181, 493, 652, 672, 701
BstMWI GCNNNNNNNGC 4 cut(s) 263, 266, 434, 443
BstNI CCWGG 2 cut(s) 156, 480
BstNSI RCATGY 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 300
BstSCI CCNGG 3 cut(s) 154, 478, 566
BstSFI CTRYAG 2 cut(s) 258, 333
BstSLI GKGCMC 2 cut(s) 90, 251
BstV1I GCAGC 8 cut(s) 161, 185, 244, 256, 278, 295, 404, 569
BstX2I RGATCY 2 cut(s) 652, 672
BstYI RGATCY 2 cut(s) 652, 672
BstZ17I GTATAC 1 cut(s) 490
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 2 cut(s) 450, 795
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 5 cut(s) 247, 262, 422, 426, 448
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 159
CviAII CATG 2 cut(s) 5, 254
CviQI GTAC 1 cut(s) 159
DpnI GATC 6 cut(s) 18, 183, 495, 654, 674, 703
DpnII GATC 6 cut(s) 16, 181, 493, 652, 672, 701
EaeI YGGCCR 1 cut(s) 793
Eco24I GRGCYC 2 cut(s) 472, 899
Eco47I GGWCC 1 cut(s) 107
Eco91I GGTNACC 1 cut(s) 300
EcoO65I GGTNACC 1 cut(s) 300
EcoRI GAATTC 2 cut(s) 431, 659
EcoRII CCWGG 2 cut(s) 154, 478
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 472, 899
FaeI CATG 2 cut(s) 8, 257
FaqI GGGAC 2 cut(s) 247, 584
FatI CATG 2 cut(s) 4, 253
FauI CCCGC 1 cut(s) 429
FauNDI CATATG 2 cut(s) 178, 439
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 8 cut(s) 150, 174, 258, 267, 270, 309, 393, 583
FriOI GRGCYC 2 cut(s) 472, 899
Fsp4HI GCNGC 8 cut(s) 150, 174, 258, 267, 270, 309, 393, 583
FspBI CTAG 3 cut(s) 170, 417, 465
GlaI GCGC 1 cut(s) 265
GluI GCNGC 8 cut(s) 150, 174, 258, 267, 270, 309, 393, 583
GsuI CTGGAG 1 cut(s) 138
HaeIII GGCC 2 cut(s) 450, 795
HapII CCGG 2 cut(s) 305, 567
HhaI GCGC 1 cut(s) 266
Hin1II CATG 2 cut(s) 8, 257
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HinfI GANTC 4 cut(s) 121, 736, 747, 763
HpaII CCGG 2 cut(s) 305, 567
HphI GGTGA 5 cut(s) 176, 212, 294, 745, 890
Hpy166II GTNNAC 3 cut(s) 490, 530, 548
Hpy188I TCNGA 1 cut(s) 344
Hpy188III TCNNGA 5 cut(s) 14, 497, 656, 744, 892
Hpy8I GTNNAC 3 cut(s) 490, 530, 548
HpyAV CCTTC 2 cut(s) 471, 639
HpyCH4III ACNGT 5 cut(s) 337, 542, 688, 820, 919
HpyCH4IV ACGT 2 cut(s) 84, 276
HpyCH4V TGCA 4 cut(s) 4, 38, 260, 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 266, 434, 443
HpySE526I ACGT 2 cut(s) 84, 276
Hsp92II CATG 2 cut(s) 8, 257
HspAI GCGC 1 cut(s) 264
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 6 cut(s) 16, 181, 493, 652, 672, 701
LmnI GCTCC 5 cut(s) 82, 157, 443, 475, 894
Lsp1109I GCAGC 8 cut(s) 161, 185, 244, 256, 278, 295, 404, 569
MaeI CTAG 3 cut(s) 170, 417, 465
MaeII ACGT 2 cut(s) 84, 276
MaeIII GTNAC 5 cut(s) 300, 688, 733, 814, 913
MalI GATC 6 cut(s) 18, 183, 495, 654, 674, 703
MboI GATC 6 cut(s) 16, 181, 493, 652, 672, 701
MboII GAAGA 3 cut(s) 68, 667, 811
MfeI CAATTG 2 cut(s) 33, 831
MflI RGATCY 2 cut(s) 652, 672
MhlI GDGCHC 4 cut(s) 90, 251, 472, 899
MluCI AATT 7 cut(s) 33, 60, 220, 431, 659, 709, 831
MlyI GAGTC 2 cut(s) 730, 741
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 2 cut(s) 100, 261
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 9, 63, 922
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 305, 567
MspR9I CCNGG 3 cut(s) 156, 480, 568
MunI CAATTG 2 cut(s) 33, 831
MvaI CCWGG 2 cut(s) 156, 480
MwoI GCNNNNNNNGC 4 cut(s) 263, 266, 434, 443
NciI CCSGG 1 cut(s) 568
NdeI CATATG 2 cut(s) 178, 439
NdeII GATC 6 cut(s) 16, 181, 493, 652, 672, 701
NlaIII CATG 2 cut(s) 8, 257
NlaIV GGNNCC 6 cut(s) 29, 78, 108, 471, 654, 896
NmeAIII GCCGAG 1 cut(s) 771
NmuCI GTSAC 3 cut(s) 300, 688, 733
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PfeI GAWTC 2 cut(s) 121, 763
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 8 cut(s) 151, 175, 259, 268, 271, 310, 394, 584
PleI GAGTC 2 cut(s) 730, 741
PpsI GAGTC 2 cut(s) 730, 741
Ppu21I YACGTR 1 cut(s) 85
PshBI ATTAAT 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 154, 478
PspEI GGTNACC 1 cut(s) 300
PspGI CCWGG 2 cut(s) 154, 478
PspN4I GGNNCC 6 cut(s) 29, 78, 108, 471, 654, 896
PspPI GGNCC 1 cut(s) 107
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 2 cut(s) 652, 672
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 3 cut(s) 9, 63, 922
SatI GCNGC 8 cut(s) 150, 174, 258, 267, 270, 309, 393, 583
Sau3AI GATC 6 cut(s) 16, 181, 493, 652, 672, 701
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 2 cut(s) 730, 741
ScrFI CCNGG 3 cut(s) 156, 480, 568
SduI GDGCHC 4 cut(s) 90, 251, 472, 899
SfcI CTRYAG 2 cut(s) 258, 333
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 7 cut(s) 33, 60, 220, 431, 659, 709, 831
SsiI CCGC 1 cut(s) 422
SspMI CTAG 3 cut(s) 170, 417, 465
StyD4I CCNGG 3 cut(s) 154, 478, 566
TaaI ACNGT 5 cut(s) 337, 542, 688, 820, 919
TaiI ACGT 2 cut(s) 87, 279
TaqI TCGA 1 cut(s) 750
TasI AATT 7 cut(s) 33, 60, 220, 431, 659, 709, 831
TfiI GAWTC 2 cut(s) 121, 763
Tru1I TTAA 3 cut(s) 9, 63, 922
Tru9I TTAA 3 cut(s) 9, 63, 922
TscAI CASTG 1 cut(s) 547
TseFI GTSAC 3 cut(s) 300, 688, 733
TseI GCWGC 8 cut(s) 149, 173, 257, 266, 269, 308, 392, 582
Tsp45I GTSAC 3 cut(s) 300, 688, 733
TspDTI ATGAA 4 cut(s) 134, 516, 618, 652
TspRI CASTG 1 cut(s) 547
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 107
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 3 cut(s) 431, 659, 709
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 3 cut(s) 170, 417, 465
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.