RchiOBHm_Chr1g0369281

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
59141039 .. 59142061
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59353

Sequence Viewer

Length: 630 bp
ATGGCATGCCCCCATGCTTCGTCTGTAGCTGCGTACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTGTCCAGTCAGCTCTCATCACTACTGCTACGCCTATGAGTGACAAAACTACCCCGCACGCTGAAATTGCATATGGAGCTGGCCTAATAAACCCTTCCAGAGCTCCATATCCTGGTTTGGTTTACGATCTTGATGTACAAGACTATGTAGATTTTTTGTGTTCACAAGGATATAGTGACAAACTATTAGAACAAATAACCAGGGACAAGACTACCTCATGCTCATCAAAATTTGATAATGAAATAGTCAATGACCTGAATTATCCTTCTTTTGCTCTTCCTATCAAGGATCCGAAATTTGTCAATGGGGTTTTCCATAGGACTGTCACTAATGTCGGATCATCAAATTCTATATACAGAGCTAAAGTGGTGGCTCCATCGGGACTCAAAATCAATGTGGATCCAAGTGTGTTGTCGTTCACATCTCTTGAGCAAAAGAAATCTTTTGTAGTCACTGTAAAAGGGCCGATTGAGAAAACAAGTATGGTCTCTGCGTCTTTGGTTTGGGACGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATATTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

209

Amino Acids

22.74

Weight (kDa)

7.69

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 51 6e-08 Subtilase family
fn3_6 PF17766 108 - 205 2.7e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 182
AciI CCGC 1 cut(s) 125
AclWI GGATC 5 cut(s) 353, 366, 415, 464, 477
AcsI RAATTY 3 cut(s) 299, 365, 415
AfaI GTAC 2 cut(s) 35, 207
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 1 cut(s) 623
AjnI CCWGG 2 cut(s) 181, 269
AluBI AGCT 5 cut(s) 29, 83, 149, 173, 431
AluI AGCT 5 cut(s) 29, 83, 149, 173, 431
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 5 cut(s) 353, 366, 415, 464, 477
AoxI GGCC 2 cut(s) 151, 533
ApeKI GCWGC 2 cut(s) 29, 68
ApoI RAATTY 3 cut(s) 299, 365, 415
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 41, 54
BamHI GGATCC 2 cut(s) 358, 469
BanII GRGCYC 2 cut(s) 175, 605
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 2 cut(s) 16, 55
BccI CCATC 2 cut(s) 454, 575
BciT130I CCWGG 2 cut(s) 183, 271
BcoDI GTCTC 1 cut(s) 562
BfmI CTRYAG 2 cut(s) 24, 626
BisI GCNGC 2 cut(s) 30, 69
BlsI GCNGC 2 cut(s) 31, 70
Bme1390I CCNGG 2 cut(s) 183, 271
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 4 cut(s) 360, 444, 471, 602
BmrFI CCNGG 2 cut(s) 183, 271
BpuEI CTTGAG 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 76
BseBI CCWGG 2 cut(s) 183, 271
BseDI CCNNGG 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 16, 55
BshFI GGCC 2 cut(s) 153, 535
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 1 cut(s) 65
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 3 cut(s) 287, 465, 590
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 3 cut(s) 287, 465, 590
BsnI GGCC 2 cut(s) 153, 535
Bso31I GGTCTC 1 cut(s) 562
Bsp1286I GDGCHC 2 cut(s) 175, 605
Bsp1407I TGTACA 1 cut(s) 205
Bsp143I GATC 4 cut(s) 196, 358, 407, 469
BspACI CCGC 1 cut(s) 125
BspANI GGCC 2 cut(s) 153, 535
BspLI GGNNCC 4 cut(s) 360, 444, 471, 602
BspPI GGATC 5 cut(s) 353, 366, 415, 464, 477
BspQI GCTCTTC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 205
BsrI ACTGG 1 cut(s) 76
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 4 cut(s) 196, 358, 407, 469
Bst2UI CCWGG 2 cut(s) 183, 271
Bst4CI ACNGT 2 cut(s) 394, 526
Bst6I CTCTTC 1 cut(s) 351
BstAUI TGTACA 1 cut(s) 205
BstC8I GCNNGC 3 cut(s) 7, 129, 151
BstEII GGTNACC 1 cut(s) 60
BstKTI GATC 4 cut(s) 199, 361, 410, 472
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 4 cut(s) 196, 358, 407, 469
BstMWI GCNNNNNNNGC 2 cut(s) 137, 146
BstNI CCWGG 2 cut(s) 183, 271
BstNSI RCATGY 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 181, 269
BstSFI CTRYAG 2 cut(s) 24, 626
BstV1I GCAGC 2 cut(s) 16, 55
BstX2I RGATCY 2 cut(s) 358, 469
BstYI RGATCY 2 cut(s) 358, 469
BsuRI GGCC 2 cut(s) 153, 535
BtsIMutI CAGTG 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 7, 129, 151
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 533
CseI GACGC 1 cut(s) 552
Csp6I GTAC 2 cut(s) 34, 206
CviAII CATG 3 cut(s) 6, 14, 288
CviQI GTAC 2 cut(s) 34, 206
DpnI GATC 4 cut(s) 198, 360, 409, 471
DpnII GATC 4 cut(s) 196, 358, 407, 469
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Ecl136II GAGCTC 1 cut(s) 173
Eco24I GRGCYC 2 cut(s) 175, 605
Eco31I GGTCTC 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 181, 269
EcoT38I GRGCYC 2 cut(s) 175, 605
FaeI CATG 3 cut(s) 9, 17, 291
FaqI GGGAC 3 cut(s) 287, 465, 590
FatI CATG 3 cut(s) 5, 13, 287
FauI CCCGC 1 cut(s) 132
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 30, 69
FriOI GRGCYC 2 cut(s) 175, 605
Fsp4HI GCNGC 2 cut(s) 30, 69
GluI GCNGC 2 cut(s) 30, 69
HaeIII GGCC 2 cut(s) 153, 535
HapII CCGG 1 cut(s) 65
HgaI GACGC 1 cut(s) 552
Hin1II CATG 3 cut(s) 9, 17, 291
HinfI GANTC 1 cut(s) 453
HpaII CCGG 1 cut(s) 65
HphI GGTGA 2 cut(s) 41, 54
Hpy166II GTNNAC 3 cut(s) 193, 233, 489
Hpy188I TCNGA 2 cut(s) 363, 407
Hpy188III TCNNGA 5 cut(s) 168, 200, 450, 497, 598
Hpy8I GTNNAC 3 cut(s) 193, 233, 489
HpyAV CCTTC 2 cut(s) 174, 345
HpyCH4III ACNGT 2 cut(s) 394, 526
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 146
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 3 cut(s) 9, 17, 291
Kzo9I GATC 4 cut(s) 196, 358, 407, 469
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 4 cut(s) 146, 178, 448, 600
Lsp1109I GCAGC 2 cut(s) 16, 55
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 5 cut(s) 60, 110, 245, 394, 520
MalI GATC 4 cut(s) 198, 360, 409, 471
MboI GATC 4 cut(s) 196, 358, 407, 469
MboII GAAGA 1 cut(s) 338
MflI RGATCY 2 cut(s) 358, 469
MhlI GDGCHC 2 cut(s) 175, 605
MluCI AATT 5 cut(s) 135, 299, 328, 365, 415
MlyI GAGTC 1 cut(s) 447
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 1 cut(s) 295
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 183, 271
MvaI CCWGG 2 cut(s) 183, 271
MwoI GCNNNNNNNGC 2 cut(s) 137, 146
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 196, 358, 407, 469
NlaIII CATG 3 cut(s) 9, 17, 291
NlaIV GGNNCC 4 cut(s) 360, 444, 471, 602
NmuCI GTSAC 5 cut(s) 60, 110, 245, 394, 520
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 351
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 182
PkrI GCNGC 2 cut(s) 31, 70
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 181, 269
PspEI GGTNACC 1 cut(s) 60
PspGI CCWGG 2 cut(s) 181, 269
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 360, 444, 471, 602
PspPI GGNCC 1 cut(s) 533
PsuI RGATCY 2 cut(s) 358, 469
RsaI GTAC 2 cut(s) 35, 207
RsaNI GTAC 2 cut(s) 34, 206
SacI GAGCTC 1 cut(s) 175
SapI GCTCTTC 1 cut(s) 351
SatI GCNGC 2 cut(s) 30, 69
Sau3AI GATC 4 cut(s) 196, 358, 407, 469
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 2 cut(s) 183, 271
SduI GDGCHC 2 cut(s) 175, 605
SetI ASST 8 cut(s) 31, 39, 85, 151, 175, 287, 327, 433
SfcI CTRYAG 2 cut(s) 24, 626
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SphI GCATGC 1 cut(s) 9
Sse9I AATT 5 cut(s) 135, 299, 328, 365, 415
SsiI CCGC 1 cut(s) 125
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 181, 269
TaaI ACNGT 2 cut(s) 394, 526
TaiI ACGT 1 cut(s) 39
TasI AATT 5 cut(s) 135, 299, 328, 365, 415
TatI WGTACW 1 cut(s) 205
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 5 cut(s) 60, 110, 245, 394, 520
TseI GCWGC 2 cut(s) 29, 68
Tsp45I GTSAC 5 cut(s) 60, 110, 245, 394, 520
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 529
Van91I CCANNNNNTGG 1 cut(s) 182
XapI RAATTY 3 cut(s) 299, 365, 415
XceI RCATGY 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.