RchiOBHm_Chr1g0325961

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
14086437 .. 14091123
4687 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55562

Sequence Viewer

Length: 2223 bp
ATGGCGCCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTTAATGTTACTCACTCAACTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCAGAATGGATGGTGTAGTGTCTATCTTCCCAAGCAAACAAAGGAGCGTGAAAACAACAAAGTCATGGAACTTCCTTGGTTTTCCAGAAACAGTTAAGAGAAGCGCCCTTGAAAGGGATATCATCATCGGTGTGATGGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATTGGAGCACAATATTACCGTGCTTCTCGATCCTTCGAAGCAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACTCATACTGCATCAACGGCAGCTGGGAACACAGTGACCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAAAAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCTGTGTACAAAGTATGCTGGAGTAGCACGCCTGCTTGTGAAGATGCTGACGTTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCCGTCGGGGGCAGTATTCCAGTTGATTATTTAAATGATGCATTTGCAATTGGGTCATTTCATGCTACTAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAGAGTTCAATTGGGTAACAGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGATGCACCCAATAAAAAAGCAGGTGCTAACGGGACCCTCTCCGGGTTTTGTCTCCAAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTGTCGATGGGGCTGGGGGTGGGGCCATATTGGGTGGTGCAGCAGGAATTGTTCTGATGGGACAAAAAGTCGCCGATGAAGTGCTTGGCCCTCTTCCCGTGCCTGCATCATACGTTGGGTTGGAAGAACACAGCGACATTTACAAATACATAAAATTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGAGGTGGCAGTAGAGCTGGATACTATTTAGAAACTGGGACATCAATGGCGTGCCCCCATGCTACAGCCATAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCCATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCTCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGAAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCAAAATATGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTTGAATTCCACATACAGAGTTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAATGTGTTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

740

Amino Acids

79.06

Weight (kDa)

8.43

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 113 1.1e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 581 6.4e-44 Subtilase family
fn3_6 PF17766 639 - 736 1.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1127
Acc36I ACCTGC 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 3 cut(s) 4, 136, 709
AccB7I CCANNNNNTGG 1 cut(s) 1778
AccI GTMKAC 1 cut(s) 816
AciI CCGC 5 cut(s) 785, 927, 1009, 1511, 1692
AclWI GGATC 4 cut(s) 570, 1946, 1959, 2008
AcoI YGGCCR 2 cut(s) 443, 924
AcsI RAATTY 5 cut(s) 438, 1091, 1730, 2008, 2143
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 138, 734, 1790
AfiI CCNNNNNNNGG 9 cut(s) 131, 405, 406, 1158, 1232, 1550, 1553, 1765, 1778
AgsI TTSAA 5 cut(s) 247, 404, 1031, 1855, 2008
AhdI GACNNNNNGTC 1 cut(s) 1292
AhlI ACTAGT 1 cut(s) 1877
AjnI CCWGG 2 cut(s) 1516, 1777
AluBI AGCT 8 cut(s) 215, 231, 459, 647, 1568, 1625, 1719, 1745
AluI AGCT 8 cut(s) 215, 231, 459, 647, 1568, 1625, 1719, 1745
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 4 cut(s) 600, 603, 1172, 2155
AlwI GGATC 4 cut(s) 570, 1946, 1959, 2008
Ama87I CYCGRG 1 cut(s) 2090
AoxI GGCC 7 cut(s) 443, 924, 1222, 1248, 1312, 1747, 2126
ApeKI GCWGC 8 cut(s) 189, 257, 644, 992, 1265, 1535, 1625, 1664
ApoI RAATTY 5 cut(s) 438, 1091, 1730, 2008, 2143
AseI ATTAAT 1 cut(s) 1425
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 2 cut(s) 7, 398
AspS9I GGNCC 8 cut(s) 479, 941, 1149, 1223, 1248, 1313, 1469, 2126
AsuC2I CCSGG 3 cut(s) 945, 1085, 1159
AsuHPI GGTGA 3 cut(s) 1012, 1637, 1650
AsuII TTCGAA 1 cut(s) 582
AvaI CYCGRG 1 cut(s) 2090
AvaII GGWCC 4 cut(s) 479, 941, 1149, 1469
BaeGI GKGCMC 2 cut(s) 1452, 1607
BaeI ACNNNNGTAYC 2 cut(s) 1828, 1861
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 1 cut(s) 1951
BanI GGYRCC 3 cut(s) 4, 136, 709
BanII GRGCYC 2 cut(s) 1771, 2198
BbsI GAAGAC 1 cut(s) 1966
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 8 cut(s) 201, 244, 656, 979, 1277, 1547, 1612, 1651
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 2 cut(s) 1518, 1779
BciVI GTATCC 3 cut(s) 79, 1565, 1829
BclI TGATCA 1 cut(s) 1194
BcnI CCSGG 3 cut(s) 945, 1085, 1159
BcoDI GTCTC 4 cut(s) 600, 603, 1172, 2155
BcuI ACTAGT 1 cut(s) 1877
BfaI CTAG 5 cut(s) 900, 1532, 1716, 1764, 1878
BfmI CTRYAG 2 cut(s) 170, 1614
BfoI RGCGCY 2 cut(s) 8, 399
BfuAI ACCTGC 1 cut(s) 1127
BfuI GTATCC 3 cut(s) 79, 1565, 1829
Bme1390I CCNGG 5 cut(s) 945, 1085, 1159, 1518, 1779
Bme18I GGWCC 4 cut(s) 479, 941, 1149, 1469
BmeRI GACNNNNNGTC 1 cut(s) 1292
BmeT110I CYCGRG 1 cut(s) 2090
BmgT120I GGNCC 8 cut(s) 479, 941, 1149, 1223, 1248, 1313, 1469, 2126
BmrFI CCNGG 5 cut(s) 945, 1085, 1159, 1518, 1779
BmrI ACTGGG 1 cut(s) 1596
BmsI GCATC 8 cut(s) 457, 644, 760, 787, 859, 1108, 1340, 1714
BmuI ACTGGG 1 cut(s) 1596
BpiI GAAGAC 1 cut(s) 1966
BpmI CTGGAG 2 cut(s) 766, 1500
Bpu10I CCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 1481
BpuMI CCSGG 3 cut(s) 945, 1085, 1159
BsaAI YACGTR 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 6 cut(s) 367, 663, 921, 974, 1084, 1866
Bsc4I CCNNNNNNNGG 9 cut(s) 131, 405, 406, 1158, 1232, 1550, 1553, 1765, 1778
Bse118I RCCGGY 1 cut(s) 1660
Bse1I ACTGG 2 cut(s) 851, 1591
Bse3DI GCAATG 3 cut(s) 267, 523, 801
BseBI CCWGG 2 cut(s) 1518, 1779
BseDI CCNNGG 6 cut(s) 367, 663, 921, 974, 1084, 1866
BseGI GGATG 3 cut(s) 307, 1668, 2176
BseLI CCNNNNNNNGG 9 cut(s) 131, 405, 406, 1158, 1232, 1550, 1553, 1765, 1778
BseMI GCAATG 3 cut(s) 267, 523, 801
BseMII CTCAG 2 cut(s) 43, 95
BseNI ACTGG 2 cut(s) 851, 1591
BseRI GAGGAG 1 cut(s) 717
BseSI GKGCMC 2 cut(s) 1452, 1607
BseXI GCAGC 8 cut(s) 201, 244, 656, 979, 1277, 1547, 1612, 1651
BseYI CCCAGC 2 cut(s) 647, 1238
BsgI GTGCAG 1 cut(s) 1284
BshFI GGCC 7 cut(s) 445, 926, 1224, 1250, 1314, 1749, 2128
BshNI GGYRCC 3 cut(s) 4, 136, 709
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 2090
BsiSI CCGG 4 cut(s) 944, 1084, 1158, 1661
BslFI GGGAC 5 cut(s) 1071, 1162, 1299, 1603, 2058
BslI CCNNNNNNNGG 9 cut(s) 131, 405, 406, 1158, 1232, 1550, 1553, 1765, 1778
BsmAI GTCTC 4 cut(s) 600, 603, 1172, 2155
BsmFI GGGAC 5 cut(s) 1071, 1162, 1299, 1603, 2058
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 7 cut(s) 445, 926, 1224, 1250, 1314, 1749, 2128
BsoBI CYCGRG 1 cut(s) 2090
Bsp119I TTCGAA 1 cut(s) 582
Bsp1286I GDGCHC 5 cut(s) 556, 1452, 1607, 1771, 2198
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
Bsp19I CCATGG 2 cut(s) 921, 974
BspACI CCGC 5 cut(s) 785, 927, 1009, 1511, 1692
BspANI GGCC 7 cut(s) 445, 926, 1224, 1250, 1314, 1749, 2128
BspCNI CTCAG 2 cut(s) 42, 94
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 4 cut(s) 570, 1946, 1959, 2008
BspT104I TTCGAA 1 cut(s) 582
BspT107I GGYRCC 3 cut(s) 4, 136, 709
BsrDI GCAATG 3 cut(s) 267, 523, 801
BsrFI RCCGGY 1 cut(s) 1660
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 851, 1591
BssAI RCCGGY 1 cut(s) 1660
BssECI CCNNGG 6 cut(s) 367, 663, 921, 974, 1084, 1866
BssMI GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 5 cut(s) 367, 663, 921, 974, 1866
Bst2UI CCWGG 2 cut(s) 1518, 1779
Bst4CI ACNGT 8 cut(s) 385, 434, 566, 658, 812, 958, 1043, 1987
Bst6I CTCTTC 3 cut(s) 15, 1323, 1413
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 722
BstBI TTCGAA 1 cut(s) 582
BstC8I GCNNGC 6 cut(s) 670, 755, 759, 1329, 1603, 1747
BstDEI CTNAG 4 cut(s) 29, 81, 489, 1180
BstDSI CCRYGG 2 cut(s) 921, 974
BstEII GGTNACC 1 cut(s) 1656
BstF5I GGATG 3 cut(s) 307, 1668, 2176
BstH2I RGCGCY 2 cut(s) 8, 399
BstHHI GCGC 2 cut(s) 7, 398
BstKTI GATC 7 cut(s) 154, 578, 1197, 1482, 1795, 1954, 2003
BstMAI GTCTC 4 cut(s) 600, 603, 1172, 2155
BstMBI GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
BstMWI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 641, 725, 750, 1625, 1733, 1742
BstNI CCWGG 2 cut(s) 1518, 1779
BstPI GGTNACC 1 cut(s) 1656
BstSCI CCNGG 5 cut(s) 943, 1083, 1157, 1516, 1777
BstSFI CTRYAG 2 cut(s) 170, 1614
BstSLI GKGCMC 2 cut(s) 1452, 1607
BstV1I GCAGC 8 cut(s) 201, 244, 656, 979, 1277, 1547, 1612, 1651
BstV2I GAAGAC 1 cut(s) 1966
BstX2I RGATCY 1 cut(s) 1951
BstYI RGATCY 1 cut(s) 1951
BsuI GTATCC 3 cut(s) 79, 1565, 1829
BsuRI GGCC 7 cut(s) 445, 926, 1224, 1250, 1314, 1749, 2128
BtgI CCRYGG 2 cut(s) 921, 974
BtsCI GGATG 3 cut(s) 307, 1668, 2176
BtsIMutI CAGTG 2 cut(s) 439, 663
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 6 cut(s) 670, 755, 759, 1329, 1603, 1747
CfoI GCGC 2 cut(s) 7, 398
Cfr10I RCCGGY 1 cut(s) 1660
Cfr13I GGNCC 8 cut(s) 479, 941, 1149, 1223, 1248, 1313, 1469, 2126
CseI GACGC 1 cut(s) 2145
Csp6I GTAC 3 cut(s) 137, 733, 1789
CviAII CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1610
CviQI GTAC 3 cut(s) 137, 733, 1789
DdeI CTNAG 4 cut(s) 29, 81, 489, 1180
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 153, 577, 1196, 1481, 1794, 1953, 2002
DpnII GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
DraI TTTAAA 1 cut(s) 864
DriI GACNNNNNGTC 1 cut(s) 1292
EaeI YGGCCR 2 cut(s) 443, 924
Eam1104I CTCTTC 3 cut(s) 15, 1323, 1413
Eam1105I GACNNNNNGTC 1 cut(s) 1292
EarI CTCTTC 3 cut(s) 15, 1323, 1413
Eco130I CCWWGG 5 cut(s) 367, 663, 921, 974, 1866
Eco24I GRGCYC 2 cut(s) 1771, 2198
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 4 cut(s) 479, 941, 1149, 1469
Eco88I CYCGRG 1 cut(s) 2090
Eco91I GGTNACC 1 cut(s) 1656
EcoO109I RGGNCCY 1 cut(s) 1149
EcoO65I GGTNACC 1 cut(s) 1656
EcoRI GAATTC 1 cut(s) 2008
EcoRII CCWGG 2 cut(s) 1516, 1777
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 5 cut(s) 367, 663, 921, 974, 1866
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 2 cut(s) 1771, 2198
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 5 cut(s) 367, 663, 921, 974, 1866
FaeI CATG 8 cut(s) 161, 212, 360, 623, 896, 925, 978, 1613
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 5 cut(s) 1071, 1162, 1299, 1603, 2058
FatI CATG 8 cut(s) 157, 208, 356, 619, 892, 921, 974, 1609
FauNDI CATATG 2 cut(s) 1444, 1630
FbaI TGATCA 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 816
FokI GGATG 3 cut(s) 314, 1655, 2183
FriOI GRGCYC 2 cut(s) 1771, 2198
FspBI CTAG 5 cut(s) 900, 1532, 1716, 1764, 1878
GlaI GCGC 2 cut(s) 6, 397
GsaI CCCAGC 2 cut(s) 651, 1242
GsuI CTGGAG 2 cut(s) 766, 1500
HaeII RGCGCY 2 cut(s) 8, 399
HaeIII GGCC 7 cut(s) 445, 926, 1224, 1250, 1314, 1749, 2128
HapII CCGG 4 cut(s) 944, 1084, 1158, 1661
HgaI GACGC 1 cut(s) 2145
HhaI GCGC 2 cut(s) 7, 398
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 161, 212, 360, 623, 896, 925, 978, 1613
Hin6I GCGC 2 cut(s) 5, 396
HinP1I GCGC 2 cut(s) 5, 396
HincII GTYRAC 2 cut(s) 184, 817
HindII GTYRAC 2 cut(s) 184, 817
HinfI GANTC 5 cut(s) 449, 596, 608, 2046, 2062
HpaII CCGG 4 cut(s) 944, 1084, 1158, 1661
HphI GGTGA 3 cut(s) 1012, 1637, 1650
Hpy166II GTNNAC 7 cut(s) 184, 733, 817, 1789, 1829, 1847, 2082
Hpy188I TCNGA 4 cut(s) 454, 1281, 1479, 1723
Hpy188III TCNNGA 5 cut(s) 83, 377, 573, 1796, 2191
Hpy8I GTNNAC 7 cut(s) 184, 733, 817, 1789, 1829, 1847, 2082
Hpy99I CGWCG 1 cut(s) 839
HpyAV CCTTC 5 cut(s) 35, 589, 932, 1770, 1938
HpyCH4III ACNGT 8 cut(s) 385, 434, 566, 658, 812, 958, 1043, 1987
HpyCH4IV ACGT 4 cut(s) 177, 721, 777, 1338
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 641, 725, 750, 1625, 1733, 1742
HpyF3I CTNAG 4 cut(s) 29, 81, 489, 1180
HpySE526I ACGT 4 cut(s) 177, 721, 777, 1338
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 161, 212, 360, 623, 896, 925, 978, 1613
HspAI GCGC 2 cut(s) 5, 396
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 1149
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 1194
Kzo9I GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
LmnI GCTCC 9 cut(s) 336, 551, 947, 1444, 1519, 1742, 1774, 2041, 2193
Lsp1109I GCAGC 8 cut(s) 201, 244, 656, 979, 1277, 1547, 1612, 1651
LweI GCATC 8 cut(s) 457, 644, 760, 787, 859, 1108, 1340, 1714
MaeI CTAG 5 cut(s) 900, 1532, 1716, 1764, 1878
MaeII ACGT 4 cut(s) 177, 721, 777, 1338
MaeIII GTNAC 9 cut(s) 64, 533, 658, 932, 958, 1037, 1656, 1987, 2113
MalI GATC 7 cut(s) 153, 577, 1196, 1481, 1794, 1953, 2002
MboI GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
MfeI CAATTG 2 cut(s) 879, 1031
MflI RGATCY 1 cut(s) 1951
MhlI GDGCHC 5 cut(s) 556, 1452, 1607, 1771, 2198
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 4 cut(s) 458, 602, 605, 2040
MmeI TCCRAC 3 cut(s) 432, 1326, 1978
Mox20I TGGCCA 1 cut(s) 445
Mph1103I ATGCAT 1 cut(s) 874
MscI TGGCCA 1 cut(s) 445
MseI TTAA 9 cut(s) 60, 387, 863, 1103, 1382, 1425, 1965, 2025, 2221
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 2 cut(s) 647, 929
MspI CCGG 4 cut(s) 944, 1084, 1158, 1661
MspR9I CCNGG 5 cut(s) 945, 1085, 1159, 1518, 1779
MunI CAATTG 2 cut(s) 879, 1031
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 2 cut(s) 1518, 1779
MwoI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 641, 725, 750, 1625, 1733, 1742
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 3 cut(s) 945, 1085, 1159
NcoI CCATGG 2 cut(s) 921, 974
NdeI CATATG 2 cut(s) 1444, 1630
NdeII GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
NlaIII CATG 8 cut(s) 161, 212, 360, 623, 896, 925, 978, 1613
NmuCI GTSAC 5 cut(s) 658, 958, 1656, 1987, 2113
NsiI ATGCAT 1 cut(s) 874
NspV TTCGAA 1 cut(s) 582
PaqCI CACCTGC 1 cut(s) 1127
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 2062
PflFI GACNNNGTC 1 cut(s) 812
PflMI CCANNNNNTGG 1 cut(s) 1778
PfoI TCCNGGA 2 cut(s) 943, 1516
PleI GAGTC 4 cut(s) 457, 602, 604, 2040
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 4 cut(s) 457, 602, 604, 2040
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 1149
PshBI ATTAAT 1 cut(s) 1425
Psp5II RGGWCCY 1 cut(s) 1149
Psp6I CCWGG 2 cut(s) 1516, 1777
PspEI GGTNACC 1 cut(s) 1656
PspFI CCCAGC 2 cut(s) 647, 1238
PspGI CCWGG 2 cut(s) 1516, 1777
PspPI GGNCC 8 cut(s) 479, 941, 1149, 1223, 1248, 1313, 1469, 2126
PspPPI RGGWCCY 1 cut(s) 1149
PsuI RGATCY 1 cut(s) 1951
PsyI GACNNNGTC 1 cut(s) 812
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 3 cut(s) 138, 734, 1790
RsaNI GTAC 3 cut(s) 137, 733, 1789
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 815
SaqAI TTAA 9 cut(s) 60, 387, 863, 1103, 1382, 1425, 1965, 2025, 2221
Sau3AI GATC 7 cut(s) 151, 575, 1194, 1479, 1792, 1951, 2000
Sau96I GGNCC 8 cut(s) 479, 941, 1149, 1223, 1248, 1313, 1469, 2126
SchI GAGTC 4 cut(s) 458, 602, 605, 2040
ScrFI CCNGG 5 cut(s) 945, 1085, 1159, 1518, 1779
SduI GDGCHC 5 cut(s) 556, 1452, 1607, 1771, 2198
SfaNI GCATC 8 cut(s) 457, 644, 760, 787, 859, 1108, 1340, 1714
SfcI CTRYAG 2 cut(s) 170, 1614
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 582
SinI GGWCC 4 cut(s) 479, 941, 1149, 1469
SmiI ATTTAAAT 1 cut(s) 864
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 1496
SmoI CTYRAG 1 cut(s) 1496
SpeI ACTAGT 1 cut(s) 1877
SsiI CCGC 5 cut(s) 785, 927, 1009, 1511, 1692
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 560
SspMI CTAG 5 cut(s) 900, 1532, 1716, 1764, 1878
StyD4I CCNGG 5 cut(s) 943, 1083, 1157, 1516, 1777
StyI CCWWGG 5 cut(s) 367, 663, 921, 974, 1866
SwaI ATTTAAAT 1 cut(s) 864
TaaI ACNGT 8 cut(s) 385, 434, 566, 658, 812, 958, 1043, 1987
TaiI ACGT 4 cut(s) 180, 724, 780, 1341
TaqI TCGA 6 cut(s) 13, 574, 582, 792, 816, 1230
TatI WGTACW 1 cut(s) 732
TauI GCSGC 3 cut(s) 788, 929, 1514
TfiI GAWTC 1 cut(s) 2062
Tru1I TTAA 9 cut(s) 60, 387, 863, 1103, 1382, 1425, 1965, 2025, 2221
Tru9I TTAA 9 cut(s) 60, 387, 863, 1103, 1382, 1425, 1965, 2025, 2221
TscAI CASTG 2 cut(s) 439, 663
TseFI GTSAC 5 cut(s) 658, 958, 1656, 1987, 2113
TseI GCWGC 8 cut(s) 189, 257, 644, 992, 1265, 1535, 1625, 1664
Tsp45I GTSAC 5 cut(s) 658, 958, 1656, 1987, 2113
TspDTI ATGAA 7 cut(s) 146, 225, 881, 1006, 1317, 1815, 1875
TspGWI ACGGA 2 cut(s) 823, 1755
TspRI CASTG 2 cut(s) 439, 663
Tth111I GACNNNGTC 1 cut(s) 812
Van91I CCANNNNNTGG 1 cut(s) 1778
VpaK11BI GGWCC 4 cut(s) 479, 941, 1149, 1469
VspI ATTAAT 1 cut(s) 1425
XapI RAATTY 5 cut(s) 438, 1091, 1730, 2008, 2143
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1030
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 5 cut(s) 900, 1532, 1716, 1764, 1878
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.