Rorug01G0354500

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000390
Physical Location & Seq
Reverse (-)
47108750 .. 47110707
1958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0354500.1

Sequence Viewer

Length: 714 bp
ATGGCTCAATCAGTTACCGGAATTCAGGTCGAAGCGACCACGTTTCCTCCCGCCGTCAAGCCTCCGGGCTCCGCCAACACTCTGTTCCTCGCCGGCGCAGGGGCGAGGGGGCTGGAGATACAGGGGAATTTCGTTAAGTTCACGGCGATTGGAGTCTACTTGGAGGATAAGGCCGTGCCGGCGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGAGATCGTTACAGGTCCATTTGAGAAATTCACACAGGTGACGATGATACTACCATTGACTGGCCAGCAATACTCGGAGAAGGTTTCAGAGAATTGTGTTGCCATTTGGAAAAAATTTGGAATTTACACTGATGCAGAAGCCAAAGCTATTGAAAAGTTTACGGAGGTCTTCAAAGATCAGACCTTCCCACCTGGTGCTTCTATTCTCTTCACACAATCACCAAATGGATCATTGACGATTGGCTTCTCCAAAGATGGTTCCATACCCGAAGTTGGGAATGCGGTGATTGAAAACAAGCTACTTTCGGAGTCAGTTCTCGAGTCAATCATAGGAAAGCAAGGTGTTTCTCCTGAAGCAAGGAAATGTGTGGCTACAAGGCTATCAGAATTGTTGAAAGAGAGTGATGATTGCGTGGCCGGAAATGGGAAAGTTGAAGAGTATACAAAGGAAGCAGAAGTTAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

237

Amino Acids

25.49

Weight (kDa)

5.01

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 10 - 212 4.2e-106 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 305
AccI GTMKAC 2 cut(s) 156, 686
AciI CCGC 3 cut(s) 51, 72, 527
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 3 cut(s) 210, 307, 660
AcsI RAATTY 5 cut(s) 21, 127, 272, 359, 366
AcuI CTGAAG 2 cut(s) 226, 618
AdeI CACNNNGTG 1 cut(s) 440
AfiI CCNNNNNNNGG 6 cut(s) 99, 195, 305, 518, 519, 669
AgsI TTSAA 5 cut(s) 398, 418, 536, 640, 680
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 2 cut(s) 392, 544
AluI AGCT 2 cut(s) 392, 544
AlwI GGATC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 4 cut(s) 171, 210, 307, 660
ApoI RAATTY 5 cut(s) 21, 127, 272, 359, 366
AspLEI GCGC 2 cut(s) 98, 184
AspS9I GGNCC 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 3 cut(s) 295, 456, 541
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 309
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 406
BccI CCATC 1 cut(s) 494
BceAI ACGGC 5 cut(s) 38, 158, 159, 173, 225
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 179
Bme1390I CCNGG 2 cut(s) 66, 438
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 70, 505
BmrFI CCNGG 2 cut(s) 66, 438
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 6 cut(s) 99, 195, 305, 518, 519, 669
Bse118I RCCGGY 2 cut(s) 92, 178
Bse1I ACTGG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 213
BseLI CCNNNNNNNGG 6 cut(s) 99, 195, 305, 518, 519, 669
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 1 cut(s) 230
BseX3I CGGCCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 4 cut(s) 173, 212, 309, 662
BsiEI CGRYCG 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 5 cut(s) 18, 65, 93, 179, 663
BslI CCNNNNNNNGG 6 cut(s) 99, 195, 305, 518, 519, 669
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 4 cut(s) 173, 212, 309, 662
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 249, 421, 473
BspACI CCGC 3 cut(s) 51, 72, 527
BspANI GGCC 4 cut(s) 173, 212, 309, 662
BspLI GGNNCC 2 cut(s) 70, 505
BspPI GGATC 1 cut(s) 481
BsrFI RCCGGY 2 cut(s) 92, 178
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 92, 178
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 249, 421, 473
BssNAI GTATAC 1 cut(s) 687
Bst1107I GTATAC 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 458, 675
BstC8I GCNNGC 4 cut(s) 94, 180, 186, 311
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 98, 184
BstKTI GATC 3 cut(s) 252, 424, 476
BstMBI GATC 3 cut(s) 249, 421, 473
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 64, 436
BstV2I GAAGAC 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 687
BstZI CGGCCG 1 cut(s) 210
BsuRI GGCC 4 cut(s) 173, 212, 309, 662
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 4 cut(s) 94, 180, 186, 311
CfoI GCGC 2 cut(s) 98, 184
Cfr10I RCCGGY 2 cut(s) 92, 178
Cfr13I GGNCC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 436
CviAII CATG 1 cut(s) 711
DpnI GATC 3 cut(s) 251, 423, 475
DpnII GATC 3 cut(s) 249, 421, 473
DraIII CACNNNGTG 1 cut(s) 440
EaeI YGGCCR 3 cut(s) 210, 307, 660
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 2 cut(s) 458, 675
EarI CTCTTC 2 cut(s) 458, 675
EciI GGCGGA 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 260
Eco52I CGGCCG 1 cut(s) 210
Eco57I CTGAAG 2 cut(s) 226, 618
Eco88I CYCGRG 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 71
FaeI CATG 1 cut(s) 714
FaiI YATR 4 cut(s) 509, 575, 687, 712
FatI CATG 1 cut(s) 710
FauI CCCGC 1 cut(s) 58
FblI GTMKAC 2 cut(s) 156, 686
FriOI GRGCYC 1 cut(s) 71
GlaI GCGC 2 cut(s) 97, 183
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 4 cut(s) 173, 212, 309, 662
HapII CCGG 5 cut(s) 18, 65, 93, 179, 663
HhaI GCGC 2 cut(s) 98, 184
Hin1II CATG 1 cut(s) 714
Hin6I GCGC 2 cut(s) 96, 182
HinP1I GCGC 2 cut(s) 96, 182
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 4 cut(s) 153, 227, 554, 566
HpaII CCGG 5 cut(s) 18, 65, 93, 179, 663
HphI GGTGA 3 cut(s) 295, 456, 541
Hpy166II GTNNAC 5 cut(s) 141, 157, 222, 405, 687
Hpy188I TCNGA 5 cut(s) 322, 334, 426, 553, 631
Hpy188III TCNNGA 2 cut(s) 563, 596
Hpy8I GTNNAC 5 cut(s) 141, 157, 222, 405, 687
HpyAV CCTTC 3 cut(s) 193, 319, 439
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 1 cut(s) 714
HspAI GCGC 2 cut(s) 96, 182
KroI GCCGGC 2 cut(s) 92, 178
KroNI GCCGGC 2 cut(s) 94, 180
Kzo9I GATC 3 cut(s) 249, 421, 473
LmnI GCTCC 1 cut(s) 74
LweI GCATC 1 cut(s) 367
MabI ACCWGGT 1 cut(s) 436
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 3 cut(s) 13, 253, 283
MalI GATC 3 cut(s) 251, 423, 475
MboI GATC 3 cut(s) 249, 421, 473
MboII GAAGA 4 cut(s) 232, 406, 445, 692
MhlI GDGCHC 1 cut(s) 71
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 4 cut(s) 162, 236, 563, 575
MnlI CCTC 7 cut(s) 57, 72, 98, 99, 157, 208, 403
Mox20I TGGCCA 1 cut(s) 309
MreI CGCCGGCG 1 cut(s) 92
MroNI GCCGGC 2 cut(s) 92, 178
MscI TGGCCA 1 cut(s) 309
MseI TTAA 3 cut(s) 135, 192, 705
MslI CAYNNNNRTG 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 309
MspI CCGG 5 cut(s) 18, 65, 93, 179, 663
MspR9I CCNGG 2 cut(s) 66, 438
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 179
NaeI GCCGGC 2 cut(s) 94, 180
NciI CCSGG 1 cut(s) 66
NdeII GATC 3 cut(s) 249, 421, 473
NgoMIV GCCGGC 2 cut(s) 92, 178
NlaIII CATG 1 cut(s) 714
NlaIV GGNNCC 2 cut(s) 70, 505
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 563
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 2 cut(s) 94, 180
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 305
PleI GAGTC 4 cut(s) 161, 235, 562, 574
PpsI GAGTC 4 cut(s) 161, 235, 562, 574
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 70, 505
PspPI GGNCC 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 3 cut(s) 135, 192, 705
Sau3AI GATC 3 cut(s) 249, 421, 473
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 4 cut(s) 162, 236, 563, 575
ScrFI CCNGG 2 cut(s) 66, 438
SduI GDGCHC 1 cut(s) 71
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 563
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
SsiI CCGC 3 cut(s) 51, 72, 527
StyD4I CCNGG 2 cut(s) 64, 436
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 30, 564
TasI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
Tru1I TTAA 3 cut(s) 135, 192, 705
Tru9I TTAA 3 cut(s) 135, 192, 705
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspGWI ACGGA 3 cut(s) 220, 239, 422
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 5 cut(s) 21, 127, 272, 359, 366
XhoI CTCGAG 1 cut(s) 563
XmiI GTMKAC 2 cut(s) 156, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.