MD07G1207100.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28625891 .. 28627965
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1207100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGAACCCAACTTTAATTTATTTTTCAACTTGCATAAACATTAATAGTGACCATTGTATGACGAGAAAAATATATGCAGGAATGGATGGTGTGGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCTCCAAACAACAAGGTCATGGGACTTCATTTGGTTTTCTGAAATGGTGAAGAGAAGCGCCATTGAAACTGACAGGGCCGGCCCAGGTCCAGTGCAGGCAGGGTCGCCCCGAGCCTCAAAAATCTCTGGACCGGGCCTGGAAACTGATATCATTGTCGGGGTGATTGACTATGGAATTTGGCCCGAATCTGCCAGCTTCAGTGACGCCGGGTTTGGTCCACCCCCCACAAGGTGGAAAGGCGCATGCAAGGGCGAAGACAATTTTACTTGCAACAATAAAATTATCGGAGCACGGTATTACCGCAGTCTACCCTACCCCAAAAATAGCAGTGATATCCTGTCTCCGAGAGACACGGAAGGCCATGGAACCCACTGTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGGGTGCCATCAGCACGCATTGCGGTGTACAAGGTTTGTTGGTCAGAAGGGTGCCTGGATGCTGATATACTAGCGGCATTCGATGATGCCATAGCTGACGGTGTTGACATACTCTCTGTCTCCCTTGGGGGGCTTAAACCGTTAGACTACTTCAGAAATTCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTTACTTCCGCATCTGCTGGTAATGAAGGTTCAAACCTGAAAACTATTACAAACTTTGCACCATGGTCTCTTGCTGTGGCTGCTAGCACCATAGATCGTCACTTTGATACCAAGGTTCAATTGGGAAACCACAAAATTTATGAGGTAATTGATACAGAATCTAACCTTTGCTCATTTAGGGCTTTGTTTGGTAGTGATTCTGGTTGGGCAATATATAATCACTTTTGGAGAATCAACAATATATATACACACACACACACACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

346

Amino Acids

37.4

Weight (kDa)

6.75

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 90 - 316 9.1e-21 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 574, 621
AccB7I CCANNNNNTGG 1 cut(s) 354
AccI GTMKAC 1 cut(s) 430
AciI CCGC 4 cut(s) 424, 593, 644, 780
AcsI RAATTY 3 cut(s) 297, 727, 906
AcuI CTGAAG 2 cut(s) 304, 706
AcyI GRCGYC 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 354
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 3 cut(s) 351, 354, 699
AgsI TTSAA 4 cut(s) 27, 188, 804, 890
AjnI CCWGG 3 cut(s) 205, 258, 624
AluBI AGCT 4 cut(s) 124, 318, 665, 746
AluI AGCT 4 cut(s) 124, 318, 665, 746
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 4 cut(s) 465, 468, 694, 843
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 5 cut(s) 198, 202, 256, 302, 481
ApeKI GCWGC 2 cut(s) 509, 851
ApoI RAATTY 3 cut(s) 297, 727, 906
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 2 cut(s) 182, 365
AspS9I GGNCC 7 cut(s) 198, 203, 209, 251, 256, 303, 338
AsuC2I CCSGG 2 cut(s) 255, 331
AsuHPI GGTGA 2 cut(s) 181, 295
AsuNHI GCTAGC 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 3 cut(s) 209, 251, 338
BaeI ACNNNNGTAYC 2 cut(s) 870, 903
BanI GGYRCC 2 cut(s) 574, 621
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 521, 838
BccI CCATC 2 cut(s) 80, 586
BciT130I CCWGG 3 cut(s) 207, 260, 626
BcnI CCSGG 2 cut(s) 255, 331
BcoDI GTCTC 4 cut(s) 465, 468, 694, 843
BfaI CTAG 3 cut(s) 108, 641, 855
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 3 cut(s) 510, 645, 852
BlsI GCNGC 3 cut(s) 511, 646, 853
Bme1390I CCNGG 5 cut(s) 207, 255, 260, 331, 626
Bme18I GGWCC 3 cut(s) 209, 251, 338
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 7 cut(s) 198, 203, 209, 251, 256, 303, 338
BmiI GGNNCC 3 cut(s) 490, 576, 623
BmrFI CCNGG 5 cut(s) 207, 255, 260, 331, 626
BmsI GCATC 4 cut(s) 509, 619, 646, 791
BmtI GCTAGC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 255, 331
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 843
BsaJI CCNNGG 5 cut(s) 205, 484, 694, 833, 882
Bsc4I CCNNNNNNNGG 3 cut(s) 351, 354, 699
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 588
BseBI CCWGG 3 cut(s) 207, 260, 626
BseDI CCNNGG 5 cut(s) 205, 484, 694, 833, 882
BseGI GGATG 2 cut(s) 91, 634
BseLI CCNNNNNNNGG 3 cut(s) 351, 354, 699
BseMI GCAATG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 582
BseXI GCAGC 2 cut(s) 521, 838
BsgI GTGCAG 1 cut(s) 236
BshFI GGCC 5 cut(s) 200, 204, 258, 304, 483
BshNI GGYRCC 2 cut(s) 574, 621
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 3 cut(s) 201, 254, 330
BslFI GGGAC 2 cut(s) 158, 571
BslI CCNNNNNNNGG 3 cut(s) 351, 354, 699
BsmAI GTCTC 4 cut(s) 465, 468, 694, 843
BsmFI GGGAC 2 cut(s) 158, 571
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 5 cut(s) 200, 204, 258, 304, 483
Bso31I GGTCTC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 415
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 1 cut(s) 865
Bsp19I CCATGG 2 cut(s) 484, 833
BspACI CCGC 4 cut(s) 424, 593, 644, 780
BspANI GGCC 5 cut(s) 200, 204, 258, 304, 483
BspLI GGNNCC 3 cut(s) 490, 576, 623
BspOI GCTAGC 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 574, 621
BspTNI GGTCTC 1 cut(s) 843
BsrDI GCAATG 1 cut(s) 588
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 205, 484, 694, 833, 882
BssMI GATC 1 cut(s) 865
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 4 cut(s) 484, 694, 833, 882
Bst2UI CCWGG 3 cut(s) 207, 260, 626
Bst4CI ACNGT 4 cut(s) 417, 497, 671, 711
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 327
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 597
BstC8I GCNNGC 6 cut(s) 202, 219, 316, 367, 586, 856
BstDEI CTNAG 2 cut(s) 117, 520
BstDSI CCRYGG 2 cut(s) 484, 833
BstF5I GGATG 2 cut(s) 91, 634
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 365
BstKTI GATC 1 cut(s) 868
BstMAI GTCTC 4 cut(s) 465, 468, 694, 843
BstMBI GATC 1 cut(s) 865
BstMWI GCNNNNNNNGC 4 cut(s) 506, 590, 752, 851
BstNI CCWGG 3 cut(s) 207, 260, 626
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 5 cut(s) 205, 253, 258, 329, 624
BstV1I GCAGC 2 cut(s) 521, 838
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 5 cut(s) 200, 204, 258, 304, 483
BtgI CCRYGG 2 cut(s) 484, 833
BtsCI GGATG 2 cut(s) 91, 634
BtsI GCAGTG 1 cut(s) 457
BtsIMutI CAGTG 4 cut(s) 219, 328, 457, 493
Cac8I GCNNGC 6 cut(s) 202, 219, 316, 367, 586, 856
CfoI GCGC 2 cut(s) 182, 365
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 7 cut(s) 198, 203, 209, 251, 256, 303, 338
CseI GACGC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 598
CviAII CATG 4 cut(s) 141, 366, 485, 834
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 2 cut(s) 117, 520
DpnI GATC 1 cut(s) 867
DpnII GATC 1 cut(s) 865
DraIII CACNNNGTG 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco130I CCWWGG 4 cut(s) 484, 694, 833, 882
Eco31I GGTCTC 1 cut(s) 843
Eco32I GATATC 2 cut(s) 271, 457
Eco47I GGWCC 3 cut(s) 209, 251, 338
Eco57I CTGAAG 2 cut(s) 304, 706
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 3 cut(s) 205, 258, 624
EcoRV GATATC 2 cut(s) 271, 457
EcoT14I CCWWGG 4 cut(s) 484, 694, 833, 882
ErhI CCWWGG 4 cut(s) 484, 694, 833, 882
FaeI CATG 4 cut(s) 144, 369, 488, 837
FaqI GGGAC 2 cut(s) 158, 571
FatI CATG 4 cut(s) 140, 365, 484, 833
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 510, 645, 852
FokI GGATG 2 cut(s) 98, 641
FseI GGCCGGCC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 510, 645, 852
FspBI CTAG 3 cut(s) 108, 641, 855
GlaI GCGC 2 cut(s) 181, 364
GluI GCNGC 3 cut(s) 510, 645, 852
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 5 cut(s) 200, 204, 258, 304, 483
HapII CCGG 3 cut(s) 201, 254, 330
HgaI GACGC 1 cut(s) 335
HhaI GCGC 2 cut(s) 182, 365
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 4 cut(s) 144, 369, 488, 837
Hin6I GCGC 2 cut(s) 180, 363
HinP1I GCGC 2 cut(s) 180, 363
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 4 cut(s) 308, 929, 968, 1002
HpaII CCGG 3 cut(s) 201, 254, 330
HphI GGTGA 2 cut(s) 181, 295
Hpy166II GTNNAC 4 cut(s) 341, 431, 598, 676
Hpy188I TCNGA 6 cut(s) 163, 410, 468, 616, 725, 1037
Hpy188III TCNNGA 1 cut(s) 249
Hpy8I GTNNAC 4 cut(s) 341, 431, 598, 676
HpyAV CCTTC 3 cut(s) 473, 611, 791
HpyCH4III ACNGT 4 cut(s) 417, 497, 671, 711
HpyCH4V TGCA 7 cut(s) 33, 77, 217, 369, 393, 500, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 590, 752, 851
HpyF3I CTNAG 2 cut(s) 117, 520
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 4 cut(s) 144, 369, 488, 837
HspAI GCGC 2 cut(s) 180, 363
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 1 cut(s) 865
LmnI GCTCC 3 cut(s) 129, 410, 743
Lsp1109I GCAGC 2 cut(s) 521, 838
LweI GCATC 4 cut(s) 509, 619, 646, 791
MaeI CTAG 3 cut(s) 108, 641, 855
MaeIII GTNAC 3 cut(s) 47, 323, 869
MalI GATC 1 cut(s) 867
MboI GATC 1 cut(s) 865
MboII GAAGA 2 cut(s) 184, 389
MfeI CAATTG 1 cut(s) 890
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 8 cut(s) 15, 297, 382, 402, 727, 890, 906, 918
MnlI CCTC 4 cut(s) 247, 560, 563, 907
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 4 cut(s) 14, 42, 705, 758
MslI CAYNNNNRTG 1 cut(s) 593
MspI CCGG 3 cut(s) 201, 254, 330
MspR9I CCNGG 5 cut(s) 207, 255, 260, 331, 626
MunI CAATTG 1 cut(s) 890
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 3 cut(s) 207, 260, 626
MwoI GCNNNNNNNGC 4 cut(s) 506, 590, 752, 851
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 2 cut(s) 255, 331
NcoI CCATGG 2 cut(s) 484, 833
NdeII GATC 1 cut(s) 865
NgoMIV GCCGGC 1 cut(s) 200
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 4 cut(s) 144, 369, 488, 837
NlaIV GGNNCC 3 cut(s) 490, 576, 623
NmuCI GTSAC 3 cut(s) 47, 323, 869
NspI RCATGY 1 cut(s) 369
PaeI GCATGC 1 cut(s) 369
PctI GAATGC 1 cut(s) 647
PdiI GCCGGC 1 cut(s) 202
PfeI GAWTC 4 cut(s) 308, 929, 968, 1002
PflMI CCANNNNNTGG 1 cut(s) 354
PkrI GCNGC 3 cut(s) 511, 646, 853
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 3 cut(s) 205, 258, 624
PspGI CCWGG 3 cut(s) 205, 258, 624
PspN4I GGNNCC 3 cut(s) 490, 576, 623
PspPI GGNCC 7 cut(s) 198, 203, 209, 251, 256, 303, 338
PsrI GAACNNNNNNTAC 2 cut(s) 784, 816
RigI GGCCGGCC 1 cut(s) 204
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 4 cut(s) 14, 42, 705, 758
SatI GCNGC 3 cut(s) 510, 645, 852
Sau3AI GATC 1 cut(s) 865
Sau96I GGNCC 7 cut(s) 198, 203, 209, 251, 256, 303, 338
ScrFI CCNGG 5 cut(s) 207, 255, 260, 331, 626
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 4 cut(s) 509, 619, 646, 791
SinI GGWCC 3 cut(s) 209, 251, 338
SmiMI CAYNNNNRTG 1 cut(s) 593
SphI GCATGC 1 cut(s) 369
Sse9I AATT 8 cut(s) 15, 297, 382, 402, 727, 890, 906, 918
SsiI CCGC 4 cut(s) 424, 593, 644, 780
SspI AATATT 1 cut(s) 771
SspMI CTAG 3 cut(s) 108, 641, 855
StyD4I CCNGG 5 cut(s) 205, 253, 258, 329, 624
StyI CCWWGG 4 cut(s) 484, 694, 833, 882
TaaI ACNGT 4 cut(s) 417, 497, 671, 711
TaqI TCGA 1 cut(s) 651
TasI AATT 8 cut(s) 15, 297, 382, 402, 727, 890, 906, 918
TatI WGTACW 1 cut(s) 597
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 4 cut(s) 308, 929, 968, 1002
Tru1I TTAA 4 cut(s) 14, 42, 705, 758
Tru9I TTAA 4 cut(s) 14, 42, 705, 758
TscAI CASTG 4 cut(s) 219, 328, 457, 500
TseFI GTSAC 3 cut(s) 47, 323, 869
TseI GCWGC 2 cut(s) 509, 851
Tsp45I GTSAC 3 cut(s) 47, 323, 869
TspDTI ATGAA 3 cut(s) 17, 139, 810
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 4 cut(s) 219, 328, 457, 500
Van91I CCANNNNNTGG 1 cut(s) 354
VpaK11BI GGWCC 3 cut(s) 209, 251, 338
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 3 cut(s) 297, 727, 906
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 430
XspI CTAG 3 cut(s) 108, 641, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.