Prupe.2G242400_v2.0.a1

Cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000390
Physical Location & Seq
Forward (+)
26139244 .. 26143077
3834 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G242400.1

Sequence Viewer

Length: 2325 bp
ATGAGCTTGCCCCATCTTTTCCAAGTAGTACGTTTTCTAGGTATTGTAGTGAGACCCCCCATAATGGATTTAGTTCCACAGTGGCCAGAAGGGTTTTTCCATGTAGTGTGGTTTCTCCTTCTCAGCCTCACATGTACTCTTCTAGTGGATGTCACTCATTCTGCTGCTCATACGGATACCAGAAAGGTTTATATTGTGTACATGGGGGACAAGCCAAAGCTCGACATTCCCACCACTACCACATTGCCTCTTCATGTAGATATGCTTCAAAACGTAGTTGGCAGCAGCAATATTGAACAAGAACCTCTCCTACTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCAAAGCTAACAGAGGAAGAAGCTCAGAAAATGGCTGGAATGGCTGGTGTGGTGTCTGTTTTCCCTAGTGGGAAGCAGAAGCTCCACACAACAAGGTCATGGAACTTCATCGGGTTTCATGAAAACGTGAAGAGAAGCACTGTTGAAAGCGATATCATCGTTGGGGTGATCGACTCTGGAGTTTGGCCGGAATCTGCCAGCTTCAGTGATGCCGGGTTCGGTCCACCACCCAAAAAATGGAAAGGCACATGCCAAGGCTCATCCAATTTTACTTGCAACAATAAAATCATTGGAGCACGGTATTACCGCAATAATGAACCCTTCGTCAAAGATGACATCAAGTCTCCGCGAGACTCGGGTGGCCATGGAACTCACACTGCATCAACGGCTGCAGGAAACTTAGTTAGCAAAGCAAGCCTATTTGGTTTGGGATCGGGGACAGCAAGAGGAGGGGTCCCATCAGCACGTATTGCGGTGTACAAAGTATGTTGGCCGAGTGATTGTGATGACGTAGATATTCTAGCGGCATTTGATGATGCGATCGCGGATGGTGTCGACATACTATCTGTCTCCCTCGGACCCGCATCGCCGGAAGATTATTTCAGGACACCAATTACCATTGGAGCCTTTCACGCTTTAAGAAAAGGAATTCTGACTTCCACTGCTGCTGGGAACGACGGTCCAGGCCCCAAGACCATCTCAAACTTTGCGCCATGGTTTCTTTCTGTGGCTGCAACCACCATAGATCGGGAGTTTGTTACCAAGGTCCAATTGGGCAACCAAAAAATCTATGAGGGAATTGTAACAAACACATTTGACCTCAAGGGCAAGTTCTATCCTCTAATATATGCTGGAGACGCACCCAATAGGACAGCAGGCTACGACGAGTCTACATCTAAGACATGCGAACCAGGCACGTTAGACCACAATTTGGTGAAGGGCAAGATTGTTCTTTGCGATGGGACGACTGGCTATGGGGCCTATTTTGCAGGTGCAGTTGGAGTTATTTTGCAAAGCCGACCTGTTGCAGATGTGCTTGACCCTCTTCCCATGCCTGCCTCTTGCCTTGGTTTGGATAGTGGTAACAGCATTTACTATTACATTACCTCAACAAGGAACCCAACTGCAACAATTTTTAAAAGTACTGAGGATATTGACACACTGTCCCCATATGTGCCATCCTTCTCATCAAGGGGTCCTAATCCAGTCACTCCCAACATTCTCAAGCCAGATATAGCTGCTCCGGGAGCTTCTATTTTGGCAGCATTTCCTCCAATTGCCCCCGTTTCGTTTGTTCAAGGAGATGACAGAGTAGCATCATACAATTTCGTCTCAGGGACATCAATGGCTTGCCCACATGCCACTGGGGTAGCTGCATACGTCAAATCATTTCACCCCAATTGGTCTCCTGCTGCTATCCAATCCGCTATTATCACCACCGCTAAACCCCTGAGTCCCGATCTTAACCCTGAAGCCGAATTTGCATATGGAGCTGGCCAAATTGACCCTGTTAGGGCTCCATATCCTGGTTTGGTATATGATGCTACGGAGCTGGACTACATAGAATTTTTGTGTGGACAAGGCTATAGTACCAAATTATTGCAATCTATTACAGGGCACAAGAGTAGCTGCTCATCAAAAACTAATTATGGAGCACTCAGTGATAATTTGAATTATCCTTCTTTTGCACTTTCCTCCTCTAATCCAAACTCCATCAGTGGGGTTTTCAATAGGACAGCCACAAATGTTGGATCACCAAGGTCCACATATAAAGCTAAAGTGATAGTTGCAACAAAGGGACTCGAAATCAAAGTTAATCCAAGCATTCTATCGTTCTCATCTCTCGGGCAGAAGCTATCCTTTCAGGTCACAGTAAAAGGGTCAATTCATCACAAATCCAGTGTCTCTGCTTCTCTGGTGTGGGATGATGGTACTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

775

Amino Acids

82.89

Weight (kDa)

6.98

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1328
Acc36I ACCTGC 1 cut(s) 1328
AccB7I CCANNNNNTGG 3 cut(s) 585, 1279, 1874
AccI GTMKAC 2 cut(s) 903, 1238
AccII CGCG 2 cut(s) 697, 893
AciI CCGC 8 cut(s) 655, 695, 821, 872, 893, 930, 1773, 1788
AclWI GGATC 2 cut(s) 787, 2107
AcoI YGGCCR 5 cut(s) 83, 533, 709, 839, 1843
AcsI RAATTY 3 cut(s) 996, 1826, 1913
AcuI CTGAAG 2 cut(s) 535, 1839
AdeI CACNNNGTG 1 cut(s) 2009
AfaI GTAC 7 cut(s) 30, 136, 200, 827, 1492, 1939, 2281
AfiI CCNNNNNNNGG 9 cut(s) 64, 585, 1095, 1279, 1420, 1461, 1861, 1874, 2067
AflIII ACRYGT 1 cut(s) 131
AgsI TTSAA 7 cut(s) 269, 296, 337, 494, 1646, 2020, 2077
AhdI GACNNNNNGTC 2 cut(s) 688, 1510
AjnI CCWGG 3 cut(s) 1030, 1258, 1873
Alw21I GWGCWC 2 cut(s) 646, 2005
Alw26I GTCTC 8 cut(s) 46, 693, 696, 922, 1197, 1684, 1758, 2257
AlwI GGATC 2 cut(s) 787, 2107
Ama87I CYCGRG 2 cut(s) 703, 2192
AoxI GGCC 7 cut(s) 83, 533, 709, 839, 1033, 1326, 1843
ApoI RAATTY 3 cut(s) 996, 1826, 1913
ArsI GACNNNNNNTTYG 2 cut(s) 2235, 2267
AsiSI GCGATCGC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 9 cut(s) 569, 802, 926, 1028, 1034, 1114, 1326, 1544, 2109
AsuC2I CCSGG 2 cut(s) 562, 1593
AsuHPI GGTGA 5 cut(s) 526, 1294, 1733, 1774, 2094
AvaI CYCGRG 2 cut(s) 703, 2192
AvaII GGWCC 7 cut(s) 569, 802, 926, 1028, 1114, 1544, 2109
BaeGI GKGCMC 1 cut(s) 1968
BalI TGGCCA 3 cut(s) 85, 711, 1845
BanII GRGCYC 2 cut(s) 1867, 2300
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
Bbv12I GWGCWC 2 cut(s) 646, 2005
BccI CCATC 8 cut(s) 21, 814, 890, 1052, 1301, 1534, 2069, 2270
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 3 cut(s) 1032, 1260, 1875
BciVI GTATCC 1 cut(s) 169
BcnI CCSGG 2 cut(s) 562, 1593
BcoDI GTCTC 8 cut(s) 46, 693, 696, 922, 1197, 1684, 1758, 2257
BfaI CTAG 4 cut(s) 38, 143, 414, 869
BfmI CTRYAG 2 cut(s) 738, 1933
BfuAI ACCTGC 1 cut(s) 1328
BfuI GTATCC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 1492
Bme1390I CCNGG 5 cut(s) 562, 1032, 1260, 1593, 1875
Bme18I GGWCC 7 cut(s) 569, 802, 926, 1028, 1114, 1544, 2109
BmeRI GACNNNNNGTC 2 cut(s) 688, 1510
BmeT110I CYCGRG 2 cut(s) 703, 2192
BmgT120I GGNCC 9 cut(s) 569, 802, 926, 1028, 1034, 1114, 1326, 1544, 2109
BmrFI CCNGG 5 cut(s) 562, 1032, 1260, 1593, 1875
BmrI ACTGGG 1 cut(s) 1722
BmsI GCATC 6 cut(s) 547, 737, 874, 941, 1673, 1879
BmuI ACTGGG 1 cut(s) 1722
BpmI CTGGAG 2 cut(s) 546, 1221
BpuEI CTTGAG 2 cut(s) 1154, 1556
BpuMI CCSGG 2 cut(s) 562, 1593
BsaAI YACGTR 1 cut(s) 815
BsaI GGTCTC 2 cut(s) 46, 1758
BsaJI CCNNGG 7 cut(s) 601, 712, 922, 1061, 1110, 1414, 2105
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 585, 1095, 1279, 1420, 1461, 1861, 1874, 2067
Bse1I ACTGG 4 cut(s) 1321, 1553, 1717, 2247
Bse3DI GCAATG 1 cut(s) 242
BseBI CCWGG 3 cut(s) 1032, 1260, 1875
BseDI CCNNGG 7 cut(s) 601, 712, 922, 1061, 1110, 1414, 2105
BseGI GGATG 5 cut(s) 154, 608, 901, 1526, 2278
BseLI CCNNNNNNNGG 9 cut(s) 64, 585, 1095, 1279, 1420, 1461, 1861, 1874, 2067
BseMI GCAATG 1 cut(s) 242
BseMII CTCAG 6 cut(s) 136, 386, 1485, 1695, 1790, 2020
BseNI ACTGG 4 cut(s) 1321, 1553, 1717, 2247
BseRI GAGGAG 2 cut(s) 810, 2035
BseSI GKGCMC 1 cut(s) 1968
BseYI CCCAGC 1 cut(s) 1016
BsgI GTGCAG 2 cut(s) 299, 1362
Bsh1236I CGCG 2 cut(s) 697, 893
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 7 cut(s) 85, 535, 711, 841, 1035, 1328, 1845
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 2 cut(s) 646, 2005
BsiHKCI CYCGRG 2 cut(s) 703, 2192
BsiSI CCGG 4 cut(s) 536, 561, 938, 1592
BslFI GGGAC 8 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788, 2160
BslI CCNNNNNNNGG 9 cut(s) 64, 585, 1095, 1279, 1420, 1461, 1861, 1874, 2067
BsmAI GTCTC 8 cut(s) 46, 693, 696, 922, 1197, 1684, 1758, 2257
BsmBI CGTCTC 2 cut(s) 1197, 1684
BsmFI GGGAC 8 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788, 2160
BsmI GAATGC 1 cut(s) 2172
BsnI GGCC 7 cut(s) 85, 535, 711, 841, 1035, 1328, 1845
Bso31I GGTCTC 2 cut(s) 46, 1758
BsoBI CYCGRG 2 cut(s) 703, 2192
Bsp1286I GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
Bsp1407I TGTACA 2 cut(s) 198, 825
Bsp143I GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
Bsp19I CCATGG 2 cut(s) 712, 1061
BspACI CCGC 8 cut(s) 655, 695, 821, 872, 893, 930, 1773, 1788
BspANI GGCC 7 cut(s) 85, 535, 711, 841, 1035, 1328, 1845
BspCNI CTCAG 6 cut(s) 135, 385, 1486, 1694, 1791, 2019
BspFNI CGCG 2 cut(s) 697, 893
BspHI TCATGA 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 1328
BspPI GGATC 2 cut(s) 787, 2107
BspTNI GGTCTC 2 cut(s) 46, 1758
BsrDI GCAATG 1 cut(s) 242
BsrGI TGTACA 2 cut(s) 198, 825
BsrI ACTGG 4 cut(s) 1321, 1553, 1717, 2247
BssECI CCNNGG 7 cut(s) 601, 712, 922, 1061, 1110, 1414, 2105
BssMI GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
BssT1I CCWWGG 6 cut(s) 601, 712, 1061, 1110, 1414, 2105
Bst2UI CCWGG 3 cut(s) 1032, 1260, 1875
Bst4CI ACNGT 6 cut(s) 81, 490, 648, 1028, 1512, 2221
Bst6I CTCTTC 4 cut(s) 144, 255, 473, 1398
BstAPI GCANNNNNTGC 1 cut(s) 818
BstAUI TGTACA 2 cut(s) 198, 825
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 7 cut(s) 8, 547, 763, 1225, 1404, 1699, 1843
BstDEI CTNAG 8 cut(s) 122, 372, 748, 1245, 1494, 1681, 1799, 2006
BstDSI CCRYGG 2 cut(s) 712, 1061
BstENI CCTNNNNNAGG 1 cut(s) 1459
BstF5I GGATG 5 cut(s) 154, 608, 901, 1526, 2278
BstFNI CGCG 2 cut(s) 697, 893
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 6 cut(s) 519, 782, 891, 1096, 1810, 2102
BstMAI GTCTC 8 cut(s) 46, 693, 696, 922, 1197, 1684, 1758, 2257
BstMBI GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
BstMCI CGRYCG 1 cut(s) 891
BstNI CCWGG 3 cut(s) 1032, 1260, 1875
BstNSI RCATGY 4 cut(s) 135, 600, 1254, 1709
BstSCI CCNGG 5 cut(s) 560, 1030, 1258, 1591, 1873
BstSFI CTRYAG 2 cut(s) 738, 1933
BstSLI GKGCMC 1 cut(s) 1968
BstUI CGCG 2 cut(s) 697, 893
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 7 cut(s) 85, 535, 711, 841, 1035, 1328, 1845
BtgI CCRYGG 2 cut(s) 712, 1061
BtgZI GCGATG 2 cut(s) 918, 1320
BtsCI GGATG 5 cut(s) 154, 608, 901, 1526, 2278
BtsI GCAGTG 2 cut(s) 723, 1008
BveI ACCTGC 1 cut(s) 1328
Cac8I GCNNGC 7 cut(s) 8, 547, 763, 1225, 1404, 1699, 1843
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 1060
Cfr13I GGNCC 9 cut(s) 569, 802, 926, 1028, 1034, 1114, 1326, 1544, 2109
CseI GACGC 1 cut(s) 1214
Csp6I GTAC 7 cut(s) 29, 135, 199, 826, 1491, 1938, 2280
CspCI CAANNNNNGTGG 2 cut(s) 2077, 2112
CviQI GTAC 7 cut(s) 29, 135, 199, 826, 1491, 1938, 2280
DdeI CTNAG 8 cut(s) 122, 372, 748, 1245, 1494, 1681, 1799, 2006
DpnI GATC 6 cut(s) 518, 781, 890, 1095, 1809, 2101
DpnII GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
DraI TTTAAA 1 cut(s) 1486
DraIII CACNNNGTG 1 cut(s) 2009
DriI GACNNNNNGTC 2 cut(s) 688, 1510
EaeI YGGCCR 5 cut(s) 83, 533, 709, 839, 1843
Eam1104I CTCTTC 4 cut(s) 144, 255, 473, 1398
Eam1105I GACNNNNNGTC 2 cut(s) 688, 1510
EarI CTCTTC 4 cut(s) 144, 255, 473, 1398
Eco130I CCWWGG 6 cut(s) 601, 712, 1061, 1110, 1414, 2105
Eco24I GRGCYC 2 cut(s) 1867, 2300
Eco31I GGTCTC 2 cut(s) 46, 1758
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 7 cut(s) 569, 802, 926, 1028, 1114, 1544, 2109
Eco57I CTGAAG 2 cut(s) 535, 1839
Eco88I CYCGRG 2 cut(s) 703, 2192
EcoNI CCTNNNNNAGG 1 cut(s) 1459
EcoO109I RGGNCCY 4 cut(s) 802, 1034, 1326, 1544
EcoRI GAATTC 1 cut(s) 996
EcoRII CCWGG 3 cut(s) 1030, 1258, 1873
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 6 cut(s) 601, 712, 1061, 1110, 1414, 2105
EcoT38I GRGCYC 2 cut(s) 1867, 2300
ErhI CCWWGG 6 cut(s) 601, 712, 1061, 1110, 1414, 2105
Esp3I CGTCTC 2 cut(s) 1197, 1684
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 2192, 2224
FaqI GGGAC 8 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788, 2160
FauI CCCGC 1 cut(s) 937
FauNDI CATATG 2 cut(s) 1519, 1834
FblI GTMKAC 2 cut(s) 903, 1238
FokI GGATG 5 cut(s) 161, 595, 908, 1513, 2285
FriOI GRGCYC 2 cut(s) 1867, 2300
FspBI CTAG 4 cut(s) 38, 143, 414, 869
GlaI GCGC 1 cut(s) 1059
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 2 cut(s) 546, 1221
HaeIII GGCC 7 cut(s) 85, 535, 711, 841, 1035, 1328, 1845
HapII CCGG 4 cut(s) 536, 561, 938, 1592
HgaI GACGC 1 cut(s) 1214
HhaI GCGC 1 cut(s) 1060
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 6 cut(s) 521, 539, 701, 1235, 1801, 2148
HpaII CCGG 4 cut(s) 536, 561, 938, 1592
HphI GGTGA 5 cut(s) 526, 1294, 1733, 1774, 2094
Hpy166II GTNNAC 7 cut(s) 199, 572, 826, 904, 1239, 1925, 2112
Hpy188I TCNGA 3 cut(s) 375, 926, 1002
Hpy188III TCNNGA 6 cut(s) 467, 525, 952, 1097, 1805, 2293
Hpy8I GTNNAC 7 cut(s) 199, 572, 826, 904, 1239, 1925, 2112
Hpy99I CGWCG 2 cut(s) 1028, 1235
HpyAV CCTTC 6 cut(s) 83, 128, 679, 1279, 1540, 2037
HpyCH4III ACNGT 6 cut(s) 81, 490, 648, 1028, 1512, 2221
HpyCH4IV ACGT 7 cut(s) 31, 273, 474, 814, 858, 1265, 1728
HpyF3I CTNAG 8 cut(s) 122, 372, 748, 1245, 1494, 1681, 1799, 2006
HpySE526I ACGT 7 cut(s) 31, 273, 474, 814, 858, 1265, 1728
HspAI GCGC 1 cut(s) 1058
KflI GGGWCCC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
LweI GCATC 6 cut(s) 547, 737, 874, 941, 1673, 1879
MaeI CTAG 4 cut(s) 38, 143, 414, 869
MaeII ACGT 7 cut(s) 31, 273, 474, 814, 858, 1265, 1728
MaeIII GTNAC 6 cut(s) 151, 1105, 1150, 1430, 1555, 2215
MalI GATC 6 cut(s) 518, 781, 890, 1095, 1809, 2101
MboI GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
MboII GAAGA 6 cut(s) 131, 242, 377, 490, 953, 1385
MfeI CAATTG 3 cut(s) 1118, 1623, 1747
MhlI GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
MlsI TGGCCA 3 cut(s) 85, 711, 1845
MluNI TGGCCA 3 cut(s) 85, 711, 1845
MlyI GAGTC 5 cut(s) 515, 695, 1244, 1810, 2142
MmeI TCCRAC 2 cut(s) 1327, 2077
Mox20I TGGCCA 3 cut(s) 85, 711, 1845
MscI TGGCCA 3 cut(s) 85, 711, 1845
MseI TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
Msp20I TGGCCA 3 cut(s) 85, 711, 1845
MspI CCGG 4 cut(s) 536, 561, 938, 1592
MspR9I CCNGG 5 cut(s) 562, 1032, 1260, 1593, 1875
MunI CAATTG 3 cut(s) 1118, 1623, 1747
Mva1269I GAATGC 1 cut(s) 2172
MvaI CCWGG 3 cut(s) 1032, 1260, 1875
MvnI CGCG 2 cut(s) 697, 893
NciI CCSGG 2 cut(s) 562, 1593
NcoI CCATGG 2 cut(s) 712, 1061
NdeI CATATG 2 cut(s) 1519, 1834
NdeII GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
NmeAIII GCCGAG 1 cut(s) 867
NmuCI GTSAC 3 cut(s) 151, 1555, 2215
NspI RCATGY 4 cut(s) 135, 600, 1254, 1709
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 1328
PciI ACATGT 1 cut(s) 131
PctI GAATGC 1 cut(s) 2172
PfeI GAWTC 1 cut(s) 539
PflMI CCANNNNNTGG 3 cut(s) 585, 1279, 1874
PfoI TCCNGGA 1 cut(s) 1591
Ple19I CGATCG 1 cut(s) 891
PleI GAGTC 5 cut(s) 515, 695, 1243, 1809, 2142
PpsI GAGTC 5 cut(s) 515, 695, 1243, 1809, 2142
Ppu21I YACGTR 1 cut(s) 815
PpuMI RGGWCCY 2 cut(s) 802, 1544
PscI ACATGT 1 cut(s) 131
Psp5II RGGWCCY 2 cut(s) 802, 1544
Psp6I CCWGG 3 cut(s) 1030, 1258, 1873
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 3 cut(s) 1030, 1258, 1873
PspPI GGNCC 9 cut(s) 569, 802, 926, 1028, 1034, 1114, 1326, 1544, 2109
PspPPI RGGWCCY 2 cut(s) 802, 1544
PsrI GAACNNNNNNTAC 2 cut(s) 294, 326
PstI CTGCAG 1 cut(s) 742
PvuI CGATCG 1 cut(s) 891
RgaI GCGATCGC 1 cut(s) 891
RsaI GTAC 7 cut(s) 30, 136, 200, 827, 1492, 1939, 2281
RsaNI GTAC 7 cut(s) 29, 135, 199, 826, 1491, 1938, 2280
SalI GTCGAC 1 cut(s) 902
SaqAI TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
Sau3AI GATC 6 cut(s) 516, 779, 888, 1093, 1807, 2099
Sau96I GGNCC 9 cut(s) 569, 802, 926, 1028, 1034, 1114, 1326, 1544, 2109
ScaI AGTACT 1 cut(s) 1492
SchI GAGTC 5 cut(s) 515, 695, 1244, 1810, 2142
ScrFI CCNGG 5 cut(s) 562, 1032, 1260, 1593, 1875
SduI GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
SfaAI GCGATCGC 1 cut(s) 891
SfaNI GCATC 6 cut(s) 547, 737, 874, 941, 1673, 1879
SfcI CTRYAG 2 cut(s) 738, 1933
SgfI GCGATCGC 1 cut(s) 891
SinI GGWCC 7 cut(s) 569, 802, 926, 1028, 1114, 1544, 2109
SmlI CTYRAG 2 cut(s) 1169, 1571
SmoI CTYRAG 2 cut(s) 1169, 1571
SsiI CCGC 8 cut(s) 655, 695, 821, 872, 893, 930, 1773, 1788
SspI AATATT 1 cut(s) 292
SspMI CTAG 4 cut(s) 38, 143, 414, 869
StyD4I CCNGG 5 cut(s) 560, 1030, 1258, 1591, 1873
StyI CCWWGG 6 cut(s) 601, 712, 1061, 1110, 1414, 2105
TaaI ACNGT 6 cut(s) 81, 490, 648, 1028, 1512, 2221
TaiI ACGT 7 cut(s) 34, 276, 477, 817, 861, 1268, 1731
TaqI TCGA 4 cut(s) 222, 519, 903, 2151
TaqII GACCGA 1 cut(s) 557
TatI WGTACW 4 cut(s) 134, 198, 825, 1490
TauI GCSGC 1 cut(s) 875
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
Tru9I TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
TseFI GTSAC 3 cut(s) 151, 1555, 2215
Tsp45I GTSAC 3 cut(s) 151, 1555, 2215
TspDTI ATGAA 6 cut(s) 242, 445, 455, 483, 678, 2225
TspGWI ACGGA 2 cut(s) 188, 1910
Van91I CCANNNNNTGG 3 cut(s) 585, 1279, 1874
VpaK11BI GGWCC 7 cut(s) 569, 802, 926, 1028, 1114, 1544, 2109
XagI CCTNNNNNAGG 1 cut(s) 1459
XapI RAATTY 3 cut(s) 996, 1826, 1913
XceI RCATGY 4 cut(s) 135, 600, 1254, 1709
XcmI CCANNNNNNNNNTGG 2 cut(s) 582, 1117
XmiI GTMKAC 2 cut(s) 903, 1238
XspI CTAG 4 cut(s) 38, 143, 414, 869
ZrmI AGTACT 1 cut(s) 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.