Rorug01G0055900

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000390
Physical Location & Seq
Forward (+)
9221063 .. 9221995
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0055900.1

Sequence Viewer

Length: 933 bp
ATGGCTTCCCACCTGGCAGAAATGCATTCACTATTATGTCTTGGGGTAGATGATGTCCGGGTAGTTGGAATATACGGAATGCCTGGTATAGGTAAAACAACCATAGCTAGAGTCGTTTTTGATGAACTTTCTTGTGAATTTGAACATTATTGCTTTCTCGAAAATGTCAAGGATGGTTTCAAGAACAACGGTGCACTACATATGCAAGAAGAACTTCTATCTAGGATCTTGGATAAAAAAGTGCGTAGCTTAGGCATTTTGAATACAGGTTTCAAGGTGGTAATGGAAAGGCTCAGTAAGAAAAAGGTTTTGCTTGTACTTGATGATGTGGAAAATTTTGCCCAAATTGAAGTCTTACTTGGGAAGCGATGTTCATTTGGTGGTGGAAGTAGAATCATTGTCACAACTAGAGATATACAATCACTAAGTGGAGTAAATGCGAGATATAGCCCCAAATTTTTAAGTGATGATGAAGCTATTGAGCTCTTTATGCAGTATGCCTTCAGAACACGCCAACCCACAGGAGAGTATGATCTTCTCTCGAGGCGTGCCACAGAATATGCTCAAGGTCTGCCTTTAGCACTTAAAGTTTTGGGAGCTTTTCTTGATAACAAAAGTATATATGAGTGGCAAGATGAGCTAGAGAAAATAAAGGACATCCCACACATAGAAATTCAAGGTGTCCTTAGAACAAGCTTTGATGGACTAGATCCTTTACAGAAGGACATATTTGTAGATATTGCATATTTCTTCAGGGGAATGGACAAAGGCTATGTAACAAAAATTTTGGAAAGTTGTGGCTTCTATCCCCATAATGGATTACGAGTACTACTTGATAGAGCTCTCATAAATATCTCATATGACAATAGGGTCGAAATGCATGATTTACTACAGGAGATAGGTTGGGAAATCGTACGCCAAGCTATCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

310

Amino Acids

35.33

Weight (kDa)

5.6

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 16 - 171 4.7e-18 NB-ARC domain
WHD_ROQ1 PF23282 240 - 307 7.1e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 869
AclWI GGATC 2 cut(s) 233, 704
AcsI RAATTY 5 cut(s) 137, 334, 455, 672, 783
AcuI CTGAAG 2 cut(s) 487, 736
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 3 cut(s) 318, 828, 915
AfiI CCNNNNNNNGG 2 cut(s) 89, 815
AgsI TTSAA 6 cut(s) 143, 181, 262, 274, 350, 677
AjnI CCWGG 2 cut(s) 12, 82
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AloI GAACNNNNNNTCC 2 cut(s) 355, 387
AluBI AGCT 9 cut(s) 107, 249, 476, 484, 599, 640, 696, 842, 923
AluI AGCT 9 cut(s) 107, 249, 476, 484, 599, 640, 696, 842, 923
Alw21I GWGCWC 3 cut(s) 196, 486, 844
Alw44I GTGCAC 1 cut(s) 192
AlwI GGATC 2 cut(s) 233, 704
Ama87I CYCGRG 1 cut(s) 541
ApaLI GTGCAC 1 cut(s) 192
ApoI RAATTY 5 cut(s) 137, 334, 455, 672, 783
Asp700I GAANNNNTTC 1 cut(s) 213
AsuC2I CCSGG 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 196
BanII GRGCYC 2 cut(s) 486, 844
Bbv12I GWGCWC 3 cut(s) 196, 486, 844
BccI CCATC 2 cut(s) 167, 695
BciT130I CCWGG 2 cut(s) 14, 84
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 5 cut(s) 108, 222, 408, 641, 707
BfmI CTRYAG 1 cut(s) 890
BmcAI AGTACT 1 cut(s) 828
Bme1390I CCNGG 3 cut(s) 14, 59, 84
BmeT110I CYCGRG 1 cut(s) 541
BmrFI CCNGG 3 cut(s) 14, 59, 84
Bpu10I CCTNAGC 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 549
BpuMI CCSGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 815
BseBI CCWGG 2 cut(s) 14, 84
BseGI GGATG 2 cut(s) 178, 657
BseLI CCNNNNNNNGG 2 cut(s) 89, 815
BseMII CTCAG 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 196
BsiHKAI GWGCWC 3 cut(s) 196, 486, 844
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 1 cut(s) 58
BsiWI CGTACG 1 cut(s) 913
BslI CCNNNNNNNGG 2 cut(s) 89, 815
BsmI GAATGC 2 cut(s) 25, 84
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 3 cut(s) 196, 486, 844
Bsp143I GATC 3 cut(s) 225, 532, 709
BspCNI CTCAG 1 cut(s) 306
BspPI GGATC 2 cut(s) 233, 704
BssMI GATC 3 cut(s) 225, 532, 709
Bst2UI CCWGG 2 cut(s) 14, 84
Bst4CI ACNGT 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 4 cut(s) 250, 293, 425, 686
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 178, 657
BstKTI GATC 3 cut(s) 228, 535, 712
BstMBI GATC 3 cut(s) 225, 532, 709
BstMWI GCNNNNNNNGC 2 cut(s) 490, 637
BstNI CCWGG 2 cut(s) 14, 84
BstSCI CCNGG 3 cut(s) 12, 57, 82
BstSFI CTRYAG 1 cut(s) 890
BstSLI GKGCMC 1 cut(s) 196
BstX2I RGATCY 2 cut(s) 225, 709
BstYI RGATCY 2 cut(s) 225, 709
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 2 cut(s) 178, 657
Cac8I GCNNGC 1 cut(s) 549
Csp6I GTAC 3 cut(s) 317, 827, 914
CviAII CATG 1 cut(s) 881
CviQI GTAC 3 cut(s) 317, 827, 914
DdeI CTNAG 4 cut(s) 250, 293, 425, 686
DpnI GATC 3 cut(s) 227, 534, 711
DpnII GATC 3 cut(s) 225, 532, 709
DraIII CACNNNGTG 1 cut(s) 428
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
Ecl136II GAGCTC 2 cut(s) 484, 842
Eco24I GRGCYC 2 cut(s) 486, 844
Eco53kI GAGCTC 2 cut(s) 484, 842
Eco57I CTGAAG 2 cut(s) 487, 736
Eco88I CYCGRG 1 cut(s) 541
EcoICRI GAGCTC 2 cut(s) 484, 842
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 12, 82
EcoT22I ATGCAT 2 cut(s) 27, 882
EcoT38I GRGCYC 2 cut(s) 486, 844
FaeI CATG 1 cut(s) 884
FalI AAGNNNNNCTT 6 cut(s) 198, 230, 342, 374, 669, 701
FatI CATG 1 cut(s) 880
FauNDI CATATG 2 cut(s) 201, 859
FokI GGATG 2 cut(s) 185, 644
FriOI GRGCYC 2 cut(s) 486, 844
FspBI CTAG 5 cut(s) 108, 222, 408, 641, 707
HapII CCGG 1 cut(s) 58
Hin1II CATG 1 cut(s) 884
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 2 cut(s) 111, 393
HpaII CCGG 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 194
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 4 cut(s) 158, 181, 541, 605
Hpy8I GTNNAC 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 511, 715
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4V TGCA 6 cut(s) 25, 194, 205, 493, 743, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 490, 637
HpyF3I CTNAG 4 cut(s) 250, 293, 425, 686
Hsp92II CATG 1 cut(s) 884
Kzo9I GATC 3 cut(s) 225, 532, 709
LmnI GCTCC 1 cut(s) 596
LpnPI CCDG 8 cut(s) 26, 69, 71, 96, 252, 507, 739, 878
MaeI CTAG 5 cut(s) 108, 222, 408, 641, 707
MaeIII GTNAC 2 cut(s) 400, 775
MalI GATC 3 cut(s) 227, 534, 711
MboI GATC 3 cut(s) 225, 532, 709
MboII GAAGA 3 cut(s) 221, 527, 742
MflI RGATCY 2 cut(s) 225, 709
MhlI GDGCHC 3 cut(s) 196, 486, 844
MluCI AATT 6 cut(s) 137, 334, 345, 455, 672, 783
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 27, 882
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 3 cut(s) 461, 585, 931
MslI CAYNNNNRTG 1 cut(s) 34
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 3 cut(s) 14, 59, 84
Mva1269I GAATGC 2 cut(s) 25, 84
MvaI CCWGG 2 cut(s) 14, 84
MwoI GCNNNNNNNGC 2 cut(s) 490, 637
NciI CCSGG 1 cut(s) 59
NdeI CATATG 2 cut(s) 201, 859
NdeII GATC 3 cut(s) 225, 532, 709
NlaIII CATG 1 cut(s) 884
NmuCI GTSAC 1 cut(s) 400
NsiI ATGCAT 2 cut(s) 27, 882
PaeR7I CTCGAG 1 cut(s) 541
PctI GAATGC 2 cut(s) 25, 84
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 393
Pfl23II CGTACG 1 cut(s) 913
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp124BI GAGCTC 2 cut(s) 486, 844
Psp6I CCWGG 2 cut(s) 12, 82
PspGI CCWGG 2 cut(s) 12, 82
PspLI CGTACG 1 cut(s) 913
PsuI RGATCY 2 cut(s) 225, 709
RsaI GTAC 3 cut(s) 318, 828, 915
RsaNI GTAC 3 cut(s) 317, 827, 914
RseI CAYNNNNRTG 1 cut(s) 34
SacI GAGCTC 2 cut(s) 486, 844
SaqAI TTAA 3 cut(s) 461, 585, 931
Sau3AI GATC 3 cut(s) 225, 532, 709
ScaI AGTACT 1 cut(s) 828
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 3 cut(s) 14, 59, 84
SduI GDGCHC 3 cut(s) 196, 486, 844
SfcI CTRYAG 1 cut(s) 890
Sfr274I CTCGAG 1 cut(s) 541
SlaI CTCGAG 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 541, 564
SmoI CTYRAG 2 cut(s) 541, 564
Sse9I AATT 6 cut(s) 137, 334, 345, 455, 672, 783
SspMI CTAG 5 cut(s) 108, 222, 408, 641, 707
SstI GAGCTC 2 cut(s) 486, 844
StyD4I CCNGG 3 cut(s) 12, 57, 82
TaaI ACNGT 1 cut(s) 191
TaqI TCGA 3 cut(s) 159, 542, 873
TasI AATT 6 cut(s) 137, 334, 345, 455, 672, 783
TatI WGTACW 2 cut(s) 316, 826
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 3 cut(s) 461, 585, 931
Tru9I TTAA 3 cut(s) 461, 585, 931
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 3 cut(s) 138, 363, 486
TspGWI ACGGA 1 cut(s) 90
VneI GTGCAC 1 cut(s) 192
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 5 cut(s) 137, 334, 455, 672, 783
XhoI CTCGAG 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 5 cut(s) 108, 222, 408, 641, 707
ZrmI AGTACT 1 cut(s) 828
Zsp2I ATGCAT 2 cut(s) 27, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.