Rh1AG189000

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000390
Physical Location & Seq
Reverse (-)
35984600 .. 35988217
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG189000.1

Sequence Viewer

Length: 2229 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTTTACTTCTTGTTCATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTAAGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTTCTTCTTCATAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGCTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAATGAGCGTCAAAACAACAAAGTCATGGAACATCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCAACCTTGAAAAAGATATCATTATCGGTGTGATCGACACTGGAATTTGGCCAGAATCGCACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTATTACCGTCATTTGGGATCCTTTGAAGAAGAAGTTGAGTCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCAATAGCAGCTGGGAACATAGTGAGAAATACGAGCATAAAAGGTTTAGGGTTTGGAAACGCAAGAGGAGGGGTGCCATCAGCACATATTGCGGTGTACAAAGCATGCTGGAAGAACGGTTGTTCAGATGCTGACATTCTAGCGGCCTTTGATGACGCCATTGCTGACGGTGTCGATATAATCTCTGCTTCCATTGCAAGCAAAGTTCCAAATGATTATTTTCGAAGCGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAAGGGGATACTAGTTTCAACTTGCAGTGGTAACGAAGGTCCGAAAAAAAGAACTGTGATAAACTTTGCACCGTGGATGCTTTCTGTGGCTGCTACCACCATAAACCGTCAGTTCATCACCAAGGTTCAATTGGGTAATGGAAAAGTCTATGAGGGACTATTACCAAACATACATGACCTCCAGGGTAAATTCTATCCTTTAATATATGGTGGAGACGCACCTAATACAACAGAAGGTGGTAACGCGACTATGTCAAGGTTCTGTTCCACAAATAGCTTAAAAGATGATTCGATCAGAGGTAAAATTGTGCTTTGCGATAGAGGCCCCGATGGCAGTGGGGATGGGTATGGGGCCATAGAGGGTGATGCAACCGGAGTTATTCTGACGGGCGTAAAAGTTGCCGAGGAGCTGGTTGGCCCTTTACCCCTGCCTGCATCTCACGTCGGATTGGAAGAAAACAGCAAAATTTACAAATACATAAGTGAAACAAGGAACCCAATTGCAACTATTTTCAAAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAATCCAATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCTCCAGGTACTCACATTCTAGCAGCATATGATCACCAACTATCAGTTGGTGATAGCAGAACTGAATATAATTTGGATAGTGGGACATCCATGGCGTGCCCTCATGCTGCAGGCGCAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCTGCTCTCATCACTACAGCTAAACCTATGAGTGCCAAAACTAGCCCGCACGCTGAATTCGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTATACGATCTTGATGAACAAGACTACTTATATTTTTTATGTTCACAAGGATACAATGGTAAACTATTGGAAATCATAACCGGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAACAGCCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTCATCAATGGGATCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCGAAATCAATGTGAATCCAAATATGCTATCATTCACATCTCTCGGCCAGAAGAAACCTTTTGTTGTTACAGTAAAAGGGTCATTTGAGAAATCAAATATAGTTTCTGCATCTTTGGTGTGGGATAATGGTGATTTCCAAGTCAGGAGCCCGATTGTTGTCTATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

742

Amino Acids

79.97

Weight (kDa)

6.85

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 7.7e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 585 2.6e-44 Subtilase family
fn3_6 PF17766 641 - 738 4.7e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 634
AccB1I GGYRCC 2 cut(s) 133, 709
AccB7I CCANNNNNTGG 2 cut(s) 495, 1784
AccI GTMKAC 1 cut(s) 1794
AccII CGCG 1 cut(s) 1142
AciI CCGC 4 cut(s) 728, 779, 864, 1727
AclWI GGATC 6 cut(s) 570, 583, 1952, 1965, 1985, 2014
AcoI YGGCCR 2 cut(s) 443, 2098
AcsI RAATTY 6 cut(s) 438, 1085, 1362, 1736, 1964, 2014
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 2 cut(s) 734, 1522
AfiI CCNNNNNNNGG 6 cut(s) 128, 495, 571, 900, 1771, 1784
AgsI TTSAA 7 cut(s) 404, 584, 915, 1025, 1411, 1679, 1950
AhlI ACTAGT 1 cut(s) 907
AjiI CACGTC 1 cut(s) 1339
AjnI CCWGG 3 cut(s) 1077, 1516, 1783
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 4 cut(s) 165, 600, 603, 1104
AlwI GGATC 6 cut(s) 570, 583, 1952, 1965, 1985, 2014
AlwNI CAGNNNCTG 1 cut(s) 767
AoxI GGCC 7 cut(s) 443, 780, 1219, 1248, 1312, 1753, 2098
ApoI RAATTY 6 cut(s) 438, 1085, 1362, 1736, 1964, 2014
AseI ATTAAT 2 cut(s) 192, 1425
Asp700I GAANNNNTTC 1 cut(s) 216
AspLEI GCGC 2 cut(s) 635, 1628
AspS9I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1469
AsuC2I CCSGG 1 cut(s) 1873
AsuHPI GGTGA 6 cut(s) 1006, 1271, 1538, 1574, 1656, 2195
AsuII TTCGAA 1 cut(s) 859
AvaII GGWCC 3 cut(s) 479, 935, 1469
BaeGI GKGCMC 2 cut(s) 1452, 1613
BaeI ACNNNNGTAYC 2 cut(s) 1834, 1867
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 575, 1957
BanI GGYRCC 2 cut(s) 133, 709
BanII GRGCYC 2 cut(s) 1777, 2204
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 556
BccI CCATC 6 cut(s) 6, 296, 721, 1220, 1232, 2053
BciT130I CCWGG 3 cut(s) 1079, 1518, 1785
BciVI GTATCC 2 cut(s) 897, 1835
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 1873
BcoDI GTCTC 4 cut(s) 165, 600, 603, 1104
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 6 cut(s) 290, 776, 908, 1532, 1722, 1770
BfmI CTRYAG 2 cut(s) 1620, 1695
BfuI GTATCC 2 cut(s) 897, 1835
BglI GCCNNNNNGGC 2 cut(s) 92, 1227
Bme1390I CCNGG 4 cut(s) 1079, 1518, 1785, 1873
Bme18I GGWCC 3 cut(s) 479, 935, 1469
BmgBI CACGTC 1 cut(s) 1339
BmgT120I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1469
BmrFI CCNGG 4 cut(s) 1079, 1518, 1785, 1873
BmsI GCATC 7 cut(s) 73, 644, 754, 963, 1252, 1340, 2171
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 2 cut(s) 1061, 1500
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 859
BpuEI CTTGAG 2 cut(s) 389, 1481
BpuMI CCSGG 1 cut(s) 1873
BsaAI YACGTR 1 cut(s) 1447
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1602, 1872
BsaWI WCCGGW 1 cut(s) 1268
BsaXI ACNNNNNCTCC 4 cut(s) 1059, 1089, 1436, 1466
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 495, 571, 900, 1771, 1784
Bse118I RCCGGY 1 cut(s) 1666
Bse1I ACTGG 2 cut(s) 137, 439
Bse3DI GCAATG 4 cut(s) 267, 628, 795, 828
BseBI CCWGG 3 cut(s) 1079, 1518, 1785
BseDI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1602, 1872
BseGI GGATG 6 cut(s) 88, 307, 363, 978, 1243, 1598
BseLI CCNNNNNNNGG 6 cut(s) 128, 495, 571, 900, 1771, 1784
BseMI GCAATG 4 cut(s) 267, 628, 795, 828
BseMII CTCAG 3 cut(s) 40, 84, 92
BseNI ACTGG 2 cut(s) 137, 439
BseRI GAGGAG 2 cut(s) 717, 1316
BseSI GKGCMC 2 cut(s) 1452, 1613
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 1142
BshFI GGCC 7 cut(s) 445, 782, 1221, 1250, 1314, 1755, 2100
BshNI GGYRCC 2 cut(s) 133, 709
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 3 cut(s) 1269, 1667, 1872
BslFI GGGAC 3 cut(s) 1065, 1609, 1889
BslI CCNNNNNNNGG 6 cut(s) 128, 495, 571, 900, 1771, 1784
BsmAI GTCTC 4 cut(s) 165, 600, 603, 1104
BsmBI CGTCTC 2 cut(s) 165, 1104
BsmFI GGGAC 3 cut(s) 1065, 1609, 1889
BsnI GGCC 7 cut(s) 445, 782, 1221, 1250, 1314, 1755, 2100
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 5 cut(s) 556, 1452, 1613, 1777, 2204
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
Bsp19I CCATGG 2 cut(s) 247, 1602
BspACI CCGC 4 cut(s) 728, 779, 864, 1727
BspANI GGCC 7 cut(s) 445, 782, 1221, 1250, 1314, 1755, 2100
BspCNI CTCAG 3 cut(s) 39, 83, 91
BspFNI CGCG 1 cut(s) 1142
BspMAI CTGCAG 1 cut(s) 1624
BspPI GGATC 6 cut(s) 570, 583, 1952, 1965, 1985, 2014
BspQI GCTCTTC 2 cut(s) 12, 222
BspT104I TTCGAA 1 cut(s) 859
BspT107I GGYRCC 2 cut(s) 133, 709
BsrDI GCAATG 4 cut(s) 267, 628, 795, 828
BsrFI RCCGGY 1 cut(s) 1666
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 137, 439
BssAI RCCGGY 1 cut(s) 1666
BssECI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1602, 1872
BssMI GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
BssNAI GTATAC 1 cut(s) 1795
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 3 cut(s) 247, 1017, 1602
Bst1107I GTATAC 1 cut(s) 1795
Bst2UI CCWGG 3 cut(s) 1079, 1518, 1785
Bst6I CTCTTC 3 cut(s) 12, 222, 1413
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 1447
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 8 cut(s) 742, 835, 1329, 1609, 1624, 1727, 1731, 1753
BstDEI CTNAG 4 cut(s) 26, 70, 78, 167
BstDSI CCRYGG 3 cut(s) 247, 968, 1602
BstEII GGTNACC 1 cut(s) 1662
BstF5I GGATG 6 cut(s) 88, 307, 363, 978, 1243, 1598
BstFNI CGCG 1 cut(s) 1142
BstHHI GCGC 2 cut(s) 635, 1628
BstKTI GATC 9 cut(s) 151, 429, 578, 1191, 1546, 1801, 1960, 1980, 2009
BstMAI GTCTC 4 cut(s) 165, 600, 603, 1104
BstMBI GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
BstNI CCWGG 3 cut(s) 1079, 1518, 1785
BstNSI RCATGY 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 1662
BstSCI CCNGG 4 cut(s) 1077, 1516, 1783, 1871
BstSFI CTRYAG 2 cut(s) 1620, 1695
BstSLI GKGCMC 2 cut(s) 1452, 1613
BstUI CGCG 1 cut(s) 1142
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 3 cut(s) 575, 1957, 1977
BstYI RGATCY 3 cut(s) 575, 1957, 1977
BstZ17I GTATAC 1 cut(s) 1795
BsuI GTATCC 2 cut(s) 897, 1835
BsuRI GGCC 7 cut(s) 445, 782, 1221, 1250, 1314, 1755, 2100
BtgI CCRYGG 3 cut(s) 247, 968, 1602
BtrI CACGTC 1 cut(s) 1339
BtsCI GGATG 6 cut(s) 88, 307, 363, 978, 1243, 1598
BtsI GCAGTG 2 cut(s) 928, 1237
BtsIMutI CAGTG 3 cut(s) 432, 928, 1237
Cac8I GCNNGC 8 cut(s) 742, 835, 1329, 1609, 1624, 1727, 1731, 1753
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 2 cut(s) 635, 1628
Cfr10I RCCGGY 1 cut(s) 1666
Cfr13I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1469
CseI GACGC 3 cut(s) 328, 800, 1121
Csp6I GTAC 2 cut(s) 733, 1521
CviQI GTAC 2 cut(s) 733, 1521
DdeI CTNAG 4 cut(s) 26, 70, 78, 167
DpnI GATC 9 cut(s) 150, 428, 577, 1190, 1545, 1800, 1959, 1979, 2008
DpnII GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
EaeI YGGCCR 2 cut(s) 443, 2098
Eam1104I CTCTTC 3 cut(s) 12, 222, 1413
EarI CTCTTC 3 cut(s) 12, 222, 1413
Eco130I CCWWGG 3 cut(s) 247, 1017, 1602
Eco24I GRGCYC 2 cut(s) 1777, 2204
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 3 cut(s) 479, 935, 1469
Eco57I CTGAAG 1 cut(s) 633
Eco91I GGTNACC 1 cut(s) 1662
EcoO109I RGGNCCY 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 1662
EcoRI GAATTC 2 cut(s) 1736, 1964
EcoRII CCWGG 3 cut(s) 1077, 1516, 1783
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 247, 1017, 1602
EcoT38I GRGCYC 2 cut(s) 1777, 2204
ErhI CCWWGG 3 cut(s) 247, 1017, 1602
Esp3I CGTCTC 2 cut(s) 165, 1104
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 3 cut(s) 1065, 1609, 1889
FauI CCCGC 1 cut(s) 1734
FauNDI CATATG 3 cut(s) 506, 1540, 1744
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 1 cut(s) 1794
FokI GGATG 6 cut(s) 95, 314, 350, 985, 1250, 1585
FriOI GRGCYC 2 cut(s) 1777, 2204
FspBI CTAG 6 cut(s) 290, 776, 908, 1532, 1722, 1770
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 2 cut(s) 634, 1627
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 1061, 1500
HaeIII GGCC 7 cut(s) 445, 782, 1221, 1250, 1314, 1755, 2100
HapII CCGG 3 cut(s) 1269, 1667, 1872
HgaI GACGC 3 cut(s) 328, 800, 1121
HhaI GCGC 2 cut(s) 635, 1628
Hin1I GRCGYC 1 cut(s) 792
Hin6I GCGC 2 cut(s) 633, 1626
HinP1I GCGC 2 cut(s) 633, 1626
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 7 cut(s) 449, 596, 608, 1184, 1483, 2052, 2068
HpaII CCGG 3 cut(s) 1269, 1667, 1872
HphI GGTGA 6 cut(s) 1006, 1271, 1538, 1574, 1656, 2195
Hpy166II GTNNAC 5 cut(s) 181, 733, 1795, 1835, 1853
Hpy188I TCNGA 7 cut(s) 551, 613, 763, 939, 1193, 1281, 1343
Hpy188III TCNNGA 6 cut(s) 80, 377, 1802, 1961, 2049, 2197
Hpy8I GTNNAC 5 cut(s) 181, 733, 1795, 1835, 1853
Hpy99I CGWCG 1 cut(s) 1343
HpyAV CCTTC 6 cut(s) 32, 608, 926, 1124, 1776, 1944
HpyCH4IV ACGT 4 cut(s) 174, 1338, 1446, 1638
HpyF3I CTNAG 4 cut(s) 26, 70, 78, 167
HpySE526I ACGT 4 cut(s) 174, 1338, 1446, 1638
Hsp92I GRCGYC 1 cut(s) 792
HspAI GCGC 2 cut(s) 633, 1626
Ksp22I TGATCA 1 cut(s) 1543
Kzo9I GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
LguI GCTCTTC 2 cut(s) 12, 222
LmnI GCTCC 8 cut(s) 551, 1303, 1444, 1519, 1748, 1780, 2047, 2199
LweI GCATC 7 cut(s) 73, 644, 754, 963, 1252, 1340, 2171
MaeI CTAG 6 cut(s) 290, 776, 908, 1532, 1722, 1770
MaeII ACGT 4 cut(s) 174, 1338, 1446, 1638
MaeIII GTNAC 9 cut(s) 61, 139, 533, 926, 1136, 1662, 1993, 2119, 2218
MalI GATC 9 cut(s) 150, 428, 577, 1190, 1545, 1800, 1959, 1979, 2008
MboI GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
MfeI CAATTG 3 cut(s) 873, 1025, 1395
MflI RGATCY 3 cut(s) 575, 1957, 1977
MhlI GDGCHC 5 cut(s) 556, 1452, 1613, 1777, 2204
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 3 cut(s) 602, 605, 2046
MmeI TCCRAC 2 cut(s) 1321, 1984
Mox20I TGGCCA 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 445
MseI TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2227
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 3 cut(s) 74, 647, 1631
MspI CCGG 3 cut(s) 1269, 1667, 1872
MspR9I CCNGG 4 cut(s) 1079, 1518, 1785, 1873
MunI CAATTG 3 cut(s) 873, 1025, 1395
MvaI CCWGG 3 cut(s) 1079, 1518, 1785
MvnI CGCG 1 cut(s) 1142
NciI CCSGG 1 cut(s) 1873
NcoI CCATGG 2 cut(s) 247, 1602
NdeI CATATG 3 cut(s) 506, 1540, 1744
NdeII GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
NmeAIII GCCGAG 2 cut(s) 1324, 2076
NmuCI GTSAC 2 cut(s) 1662, 1993
NsbI TGCGCA 1 cut(s) 634
NspI RCATGY 1 cut(s) 744
NspV TTCGAA 1 cut(s) 859
PaeI GCATGC 1 cut(s) 744
PciSI GCTCTTC 2 cut(s) 12, 222
PcsI WCGNNNNNNNCGW 2 cut(s) 426, 562
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 4 cut(s) 449, 1184, 1483, 2068
PflFI GACNNNGTC 2 cut(s) 806, 1147
PflMI CCANNNNNTGG 2 cut(s) 495, 1784
PleI GAGTC 3 cut(s) 602, 604, 2046
PpsI GAGTC 3 cut(s) 602, 604, 2046
Ppu21I YACGTR 1 cut(s) 1447
PshBI ATTAAT 2 cut(s) 192, 1425
Psp6I CCWGG 3 cut(s) 1077, 1516, 1783
PspEI GGTNACC 1 cut(s) 1662
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 3 cut(s) 1077, 1516, 1783
PspPI GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1469
PstI CTGCAG 1 cut(s) 1624
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 3 cut(s) 575, 1957, 1977
PsyI GACNNNGTC 2 cut(s) 806, 1147
PvuII CAGCTG 3 cut(s) 74, 647, 1631
RsaI GTAC 2 cut(s) 734, 1522
RsaNI GTAC 2 cut(s) 733, 1521
SapI GCTCTTC 2 cut(s) 12, 222
SaqAI TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2227
Sau3AI GATC 9 cut(s) 148, 426, 575, 1188, 1543, 1798, 1957, 1977, 2006
Sau96I GGNCC 6 cut(s) 479, 935, 1220, 1248, 1313, 1469
SchI GAGTC 3 cut(s) 602, 605, 2046
ScrFI CCNGG 4 cut(s) 1079, 1518, 1785, 1873
SduI GDGCHC 5 cut(s) 556, 1452, 1613, 1777, 2204
SfaNI GCATC 7 cut(s) 73, 644, 754, 963, 1252, 1340, 2171
SfcI CTRYAG 2 cut(s) 1620, 1695
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 3 cut(s) 479, 935, 1469
SmlI CTYRAG 2 cut(s) 368, 1496
SmoI CTYRAG 2 cut(s) 368, 1496
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 1 cut(s) 744
SsiI CCGC 4 cut(s) 728, 779, 864, 1727
SspI AATATT 1 cut(s) 163
SspMI CTAG 6 cut(s) 290, 776, 908, 1532, 1722, 1770
StyD4I CCNGG 4 cut(s) 1077, 1516, 1783, 1871
StyI CCWWGG 3 cut(s) 247, 1017, 1602
TaiI ACGT 4 cut(s) 177, 1341, 1449, 1641
TaqI TCGA 6 cut(s) 10, 429, 810, 859, 1187, 2055
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 4 cut(s) 449, 1184, 1483, 2068
Tru1I TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2227
Tru9I TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2227
TscAI CASTG 3 cut(s) 439, 928, 1237
TseFI GTSAC 2 cut(s) 1662, 1993
Tsp45I GTSAC 2 cut(s) 1662, 1993
TspDTI ATGAA 9 cut(s) 47, 143, 215, 225, 1000, 1496, 1821, 1923, 1957
TspRI CASTG 3 cut(s) 439, 928, 1237
Tth111I GACNNNGTC 2 cut(s) 806, 1147
Van91I CCANNNNNTGG 2 cut(s) 495, 1784
VpaK11BI GGWCC 3 cut(s) 479, 935, 1469
VspI ATTAAT 2 cut(s) 192, 1425
XapI RAATTY 6 cut(s) 438, 1085, 1362, 1736, 1964, 2014
XceI RCATGY 1 cut(s) 744
XcmI CCANNNNNNNNNTGG 3 cut(s) 492, 1024, 1556
XmiI GTMKAC 1 cut(s) 1794
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 6 cut(s) 290, 776, 908, 1532, 1722, 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.