Rh1BG326400

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000390
Physical Location & Seq
Reverse (-)
45858281 .. 45863209
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG326400.1

Sequence Viewer

Length: 1728 bp
ATGCCTCTAGCTAGTAGCACCATACAGAGTAATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAGTATTCTACTGCTTGTCGATGTTACTCATTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATATGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAGAGGAGCGTCAAAACAACAAAGTCATGGAACTTTCTTGGGTTTCCAGAAACAGTTAAGAGAGGTGCCCTTGAAAGGGATATCATTGTTGGTGTGATAGATAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCAAAATATTATCGTGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGATCAAGGCGAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATGTTCTAGCCGCATTCGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCGTCGGGGGCACTATTCCATTTGATTATTTGAATGATCCATTTGCAATTGGGTCATTTCACGCTACTGGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGCGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATAAGGGACTTTTACCAAACATATATGGCCTCCAGGGTCAATTTTACAAAAGCAGGTTTTGTGGAAGAGATGACTTAGACGAAGATTTGATCAAAGGTAAAATTGTGCTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCCATATTGGGTGGCGCAGCCGGAATTGTTCTGACAGGAAAAAAAGTCGACGATGAACTACTTGGCCCTTATCCGGTGCCTGCATCTTTCGTCGGGTTGGAAGAAAACAGCAACATTTACAAATACATAAATACAACAAGGAATGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCACCATACGTGCCATCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGACGGATACTATATAGACACTGGGACATCAATGGCATGCCCTCATGCTACGGCCACAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACTGGTTTGGTTCTAAAGGCAATCTACTGTGAGATTCACCTCAATAATTCAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

575

Amino Acids

61.22

Weight (kDa)

6.56

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 124 5.7e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 147 - 557 7.1e-40 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1128
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 5 cut(s) 169, 428, 742, 1303, 1417
AccB7I CCANNNNNTGG 2 cut(s) 528, 1532
AccI GTMKAC 2 cut(s) 849, 1275
AccII CGCG 1 cut(s) 754
AciI CCGC 6 cut(s) 150, 666, 819, 960, 1042, 1490
AclWI GGATC 1 cut(s) 896
AcoI YGGCCR 3 cut(s) 476, 957, 1596
AcsI RAATTY 1 cut(s) 471
AfaI GTAC 3 cut(s) 171, 767, 1724
AfiI CCNNNNNNNGG 7 cut(s) 164, 438, 439, 528, 1300, 1529, 1532
AgsI TTSAA 6 cut(s) 280, 437, 616, 898, 1064, 1719
AjnI CCWGG 2 cut(s) 1116, 1495
AluBI AGCT 7 cut(s) 11, 110, 264, 492, 680, 705, 1604
AluI AGCT 7 cut(s) 11, 110, 264, 492, 680, 705, 1604
Alw26I GTCTC 3 cut(s) 633, 636, 1539
AlwI GGATC 1 cut(s) 896
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 8 cut(s) 476, 957, 1111, 1201, 1227, 1291, 1447, 1596
ApaI GGGCCC 1 cut(s) 1451
ApeKI GCWGC 9 cut(s) 110, 290, 677, 783, 1025, 1244, 1514, 1604, 1643
ApoI RAATTY 1 cut(s) 471
Asp718I GGTACC 1 cut(s) 169
AspLEI GCGC 3 cut(s) 754, 983, 1244
AspS9I GGNCC 7 cut(s) 512, 974, 1202, 1227, 1292, 1447, 1448
AsuC2I CCSGG 1 cut(s) 978
AsuHPI GGTGA 4 cut(s) 1045, 1616, 1629, 1697
AvaII GGWCC 2 cut(s) 512, 974
BaeGI GKGCMC 3 cut(s) 433, 878, 1451
BalI TGGCCA 1 cut(s) 478
BanI GGYRCC 5 cut(s) 169, 428, 742, 1303, 1417
BanII GRGCYC 1 cut(s) 1451
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 9 cut(s) 97, 277, 689, 795, 1012, 1256, 1526, 1591, 1630
BccI CCATC 5 cut(s) 42, 329, 754, 1193, 1438
BceAI ACGGC 1 cut(s) 1611
BciT130I CCWGG 2 cut(s) 1118, 1497
BciVI GTATCC 1 cut(s) 1544
BclI TGATCA 2 cut(s) 693, 1173
BcnI CCSGG 1 cut(s) 978
BcoDI GTCTC 3 cut(s) 633, 636, 1539
BfaI CTAG 4 cut(s) 8, 12, 815, 1726
BfuAI ACCTGC 1 cut(s) 1128
BfuI GTATCC 1 cut(s) 1544
BglI GCCNNNNNGGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 1724
Bme1390I CCNGG 3 cut(s) 978, 1118, 1497
Bme18I GGWCC 2 cut(s) 512, 974
BmgT120I GGNCC 7 cut(s) 512, 974, 1202, 1227, 1292, 1447, 1448
BmrFI CCNGG 3 cut(s) 978, 1118, 1497
BmrI ACTGGG 1 cut(s) 1575
BmsI GCATC 6 cut(s) 109, 490, 677, 793, 820, 1319
BmuI ACTGGG 1 cut(s) 1575
BpiI GAAGAC 1 cut(s) 343
BpmI CTGGAG 2 cut(s) 1100, 1479
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 1460
BpuMI CCSGG 1 cut(s) 978
BsaAI YACGTR 1 cut(s) 1426
BsaJI CCNNGG 4 cut(s) 954, 1007, 1056, 1117
BsaWI WCCGGW 1 cut(s) 1300
Bsc4I CCNNNNNNNGG 7 cut(s) 164, 438, 439, 528, 1300, 1529, 1532
Bse118I RCCGGY 1 cut(s) 1639
Bse1I ACTGG 3 cut(s) 937, 1570, 1675
Bse3DI GCAATG 3 cut(s) 300, 556, 834
BseBI CCWGG 2 cut(s) 1118, 1497
BseDI CCNNGG 4 cut(s) 954, 1007, 1056, 1117
BseGI GGATG 4 cut(s) 124, 340, 1216, 1430
BseLI CCNNNNNNNGG 7 cut(s) 164, 438, 439, 528, 1300, 1529, 1532
BseMI GCAATG 3 cut(s) 300, 556, 834
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 3 cut(s) 937, 1570, 1675
BseRI GAGGAG 2 cut(s) 382, 750
BseSI GKGCMC 3 cut(s) 433, 878, 1451
BseXI GCAGC 9 cut(s) 97, 277, 689, 795, 1012, 1256, 1526, 1591, 1630
BseYI CCCAGC 2 cut(s) 680, 1217
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 8 cut(s) 478, 959, 1113, 1203, 1229, 1293, 1449, 1598
BshNI GGYRCC 5 cut(s) 169, 428, 742, 1303, 1417
BsiSI CCGG 4 cut(s) 977, 1248, 1301, 1640
BslFI GGGAC 3 cut(s) 498, 1104, 1582
BslI CCNNNNNNNGG 7 cut(s) 164, 438, 439, 528, 1300, 1529, 1532
BsmAI GTCTC 3 cut(s) 633, 636, 1539
BsmBI CGTCTC 1 cut(s) 1539
BsmFI GGGAC 3 cut(s) 498, 1104, 1582
BsmI GAATGC 2 cut(s) 821, 1375
BsnI GGCC 8 cut(s) 478, 959, 1113, 1203, 1229, 1293, 1449, 1598
Bsp120I GGGCCC 1 cut(s) 1447
Bsp1286I GDGCHC 3 cut(s) 433, 878, 1451
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
Bsp19I CCATGG 2 cut(s) 954, 1007
BspACI CCGC 6 cut(s) 150, 666, 819, 960, 1042, 1490
BspANI GGCC 8 cut(s) 478, 959, 1113, 1203, 1229, 1293, 1449, 1598
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 1128
BspPI GGATC 1 cut(s) 896
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 5 cut(s) 169, 428, 742, 1303, 1417
BsrDI GCAATG 3 cut(s) 300, 556, 834
BsrFI RCCGGY 1 cut(s) 1639
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 3 cut(s) 937, 1570, 1675
BssAI RCCGGY 1 cut(s) 1639
BssECI CCNNGG 4 cut(s) 954, 1007, 1056, 1117
BssMI GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
BssT1I CCWWGG 3 cut(s) 954, 1007, 1056
Bst2UI CCWGG 2 cut(s) 1118, 1497
Bst4CI ACNGT 8 cut(s) 221, 418, 650, 691, 845, 991, 1076, 1697
Bst6I CTCTTC 3 cut(s) 48, 1144, 1392
BstAUI TGTACA 1 cut(s) 765
BstBAI YACGTR 1 cut(s) 1426
BstC8I GCNNGC 4 cut(s) 155, 703, 1308, 1582
BstDEI CTNAG 2 cut(s) 114, 1159
BstDSI CCRYGG 2 cut(s) 954, 1007
BstEII GGTNACC 1 cut(s) 1635
BstF5I GGATG 4 cut(s) 124, 340, 1216, 1430
BstFNI CGCG 1 cut(s) 754
BstHHI GCGC 3 cut(s) 754, 983, 1244
BstKTI GATC 6 cut(s) 187, 250, 696, 904, 1176, 1461
BstMAI GTCTC 3 cut(s) 633, 636, 1539
BstMBI GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
BstMWI GCNNNNNNNGC 8 cut(s) 119, 128, 304, 674, 751, 758, 789, 1604
BstNI CCWGG 2 cut(s) 1118, 1497
BstNSI RCATGY 2 cut(s) 777, 1584
BstPI GGTNACC 1 cut(s) 1635
BstSCI CCNGG 3 cut(s) 976, 1116, 1495
BstSLI GKGCMC 3 cut(s) 433, 878, 1451
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 9 cut(s) 97, 277, 689, 795, 1012, 1256, 1526, 1591, 1630
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuI GTATCC 1 cut(s) 1544
BsuRI GGCC 8 cut(s) 478, 959, 1113, 1203, 1229, 1293, 1449, 1598
BtgI CCRYGG 2 cut(s) 954, 1007
BtsCI GGATG 4 cut(s) 124, 340, 1216, 1430
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 3 cut(s) 696, 793, 1563
BveI ACCTGC 1 cut(s) 1128
Cac8I GCNNGC 4 cut(s) 155, 703, 1308, 1582
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 3 cut(s) 754, 983, 1244
Cfr10I RCCGGY 1 cut(s) 1639
Cfr13I GGNCC 7 cut(s) 512, 974, 1202, 1227, 1292, 1447, 1448
CseI GACGC 1 cut(s) 361
Csp6I GTAC 3 cut(s) 170, 766, 1723
CviAII CATG 8 cut(s) 242, 390, 653, 774, 955, 1008, 1581, 1589
CviQI GTAC 3 cut(s) 170, 766, 1723
DdeI CTNAG 2 cut(s) 114, 1159
DpnI GATC 6 cut(s) 186, 249, 695, 903, 1175, 1460
DpnII GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
EaeI YGGCCR 3 cut(s) 476, 957, 1596
Eam1104I CTCTTC 3 cut(s) 48, 1144, 1392
EarI CTCTTC 3 cut(s) 48, 1144, 1392
Eco130I CCWWGG 3 cut(s) 954, 1007, 1056
Eco24I GRGCYC 1 cut(s) 1451
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 512, 974
Eco91I GGTNACC 1 cut(s) 1635
EcoO109I RGGNCCY 1 cut(s) 1447
EcoO65I GGTNACC 1 cut(s) 1635
EcoRII CCWGG 2 cut(s) 1116, 1495
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 3 cut(s) 954, 1007, 1056
EcoT38I GRGCYC 1 cut(s) 1451
ErhI CCWWGG 3 cut(s) 954, 1007, 1056
Esp3I CGTCTC 1 cut(s) 1539
FaeI CATG 8 cut(s) 245, 393, 656, 777, 958, 1011, 1584, 1592
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 689, 721
FaqI GGGAC 3 cut(s) 498, 1104, 1582
FatI CATG 8 cut(s) 241, 389, 652, 773, 954, 1007, 1580, 1588
FauNDI CATATG 2 cut(s) 192, 1609
FbaI TGATCA 2 cut(s) 693, 1173
FblI GTMKAC 2 cut(s) 849, 1275
FokI GGATG 4 cut(s) 131, 347, 1223, 1417
FriOI GRGCYC 1 cut(s) 1451
FspBI CTAG 4 cut(s) 8, 12, 815, 1726
GlaI GCGC 3 cut(s) 753, 982, 1243
GsaI CCCAGC 2 cut(s) 684, 1221
GsuI CTGGAG 2 cut(s) 1100, 1479
HaeIII GGCC 8 cut(s) 478, 959, 1113, 1203, 1229, 1293, 1449, 1598
HapII CCGG 4 cut(s) 977, 1248, 1301, 1640
HgaI GACGC 1 cut(s) 361
HhaI GCGC 3 cut(s) 754, 983, 1244
Hin1II CATG 8 cut(s) 245, 393, 656, 777, 958, 1011, 1584, 1592
Hin6I GCGC 3 cut(s) 752, 981, 1242
HinP1I GCGC 3 cut(s) 752, 981, 1242
HincII GTYRAC 2 cut(s) 217, 1276
HindII GTYRAC 2 cut(s) 217, 1276
HinfI GANTC 4 cut(s) 482, 629, 641, 1702
HpaII CCGG 4 cut(s) 977, 1248, 1301, 1640
HphI GGTGA 4 cut(s) 1045, 1616, 1629, 1697
Hpy166II GTNNAC 4 cut(s) 217, 766, 850, 1276
Hpy188I TCNGA 4 cut(s) 487, 584, 1260, 1458
Hpy188III TCNNGA 3 cut(s) 116, 410, 723
Hpy8I GTNNAC 4 cut(s) 217, 766, 850, 1276
Hpy99I CGWCG 3 cut(s) 872, 1280, 1322
HpyAV CCTTC 2 cut(s) 68, 965
HpyCH4III ACNGT 8 cut(s) 221, 418, 650, 691, 845, 991, 1076, 1697
HpyCH4IV ACGT 2 cut(s) 210, 1425
HpyCH4V TGCA 9 cut(s) 75, 239, 293, 911, 1004, 1310, 1373, 1379, 1607
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 128, 304, 674, 751, 758, 789, 1604
HpyF3I CTNAG 2 cut(s) 114, 1159
HpySE526I ACGT 2 cut(s) 210, 1425
Hsp92II CATG 8 cut(s) 245, 393, 656, 777, 958, 1011, 1584, 1592
HspAI GCGC 3 cut(s) 752, 981, 1242
KpnI GGTACC 1 cut(s) 173
Ksp22I TGATCA 2 cut(s) 693, 1173
Kzo9I GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 3 cut(s) 369, 584, 1498
Lsp1109I GCAGC 9 cut(s) 97, 277, 689, 795, 1012, 1256, 1526, 1591, 1630
LweI GCATC 6 cut(s) 109, 490, 677, 793, 820, 1319
MaeI CTAG 4 cut(s) 8, 12, 815, 1726
MaeII ACGT 2 cut(s) 210, 1425
MaeIII GTNAC 7 cut(s) 97, 566, 797, 965, 991, 1070, 1635
MalI GATC 6 cut(s) 186, 249, 695, 903, 1175, 1460
MboI GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
MfeI CAATTG 2 cut(s) 912, 1064
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 3 cut(s) 433, 878, 1451
MlsI TGGCCA 1 cut(s) 478
MluCI AATT 8 cut(s) 471, 912, 1046, 1064, 1124, 1185, 1251, 1714
MluNI TGGCCA 1 cut(s) 478
MlyI GAGTC 3 cut(s) 491, 635, 638
MmeI TCCRAC 2 cut(s) 465, 1305
Mox20I TGGCCA 1 cut(s) 478
MscI TGGCCA 1 cut(s) 478
MseI TTAA 2 cut(s) 420, 708
Msp20I TGGCCA 1 cut(s) 478
MspA1I CMGCKG 4 cut(s) 110, 680, 962, 1604
MspI CCGG 4 cut(s) 977, 1248, 1301, 1640
MspR9I CCNGG 3 cut(s) 978, 1118, 1497
MunI CAATTG 2 cut(s) 912, 1064
Mva1269I GAATGC 2 cut(s) 821, 1375
MvaI CCWGG 2 cut(s) 1118, 1497
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 8 cut(s) 119, 128, 304, 674, 751, 758, 789, 1604
NciI CCSGG 1 cut(s) 978
NcoI CCATGG 2 cut(s) 954, 1007
NdeI CATATG 2 cut(s) 192, 1609
NdeII GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
NlaIII CATG 8 cut(s) 245, 393, 656, 777, 958, 1011, 1584, 1592
NmuCI GTSAC 3 cut(s) 797, 991, 1635
NspI RCATGY 2 cut(s) 777, 1584
PaeI GCATGC 1 cut(s) 1584
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 2 cut(s) 821, 1375
PfeI GAWTC 1 cut(s) 1702
PflMI CCANNNNNTGG 2 cut(s) 528, 1532
PfoI TCCNGGA 1 cut(s) 1495
PleI GAGTC 3 cut(s) 490, 635, 637
PpsI GAGTC 3 cut(s) 490, 635, 637
Ppu21I YACGTR 1 cut(s) 1426
Psp6I CCWGG 2 cut(s) 1116, 1495
PspEI GGTNACC 1 cut(s) 1635
PspFI CCCAGC 2 cut(s) 680, 1217
PspGI CCWGG 2 cut(s) 1116, 1495
PspOMI GGGCCC 1 cut(s) 1447
PspPI GGNCC 7 cut(s) 512, 974, 1202, 1227, 1292, 1447, 1448
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 3 cut(s) 110, 680, 1604
RsaI GTAC 3 cut(s) 171, 767, 1724
RsaNI GTAC 3 cut(s) 170, 766, 1723
SalI GTCGAC 1 cut(s) 1274
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 2 cut(s) 420, 708
Sau3AI GATC 6 cut(s) 184, 247, 693, 901, 1173, 1458
Sau96I GGNCC 7 cut(s) 512, 974, 1202, 1227, 1292, 1447, 1448
ScaI AGTACT 1 cut(s) 1724
SchI GAGTC 3 cut(s) 491, 635, 638
ScrFI CCNGG 3 cut(s) 978, 1118, 1497
SduI GDGCHC 3 cut(s) 433, 878, 1451
SfaNI GCATC 6 cut(s) 109, 490, 677, 793, 820, 1319
SinI GGWCC 2 cut(s) 512, 974
SmlI CTYRAG 1 cut(s) 1475
SmoI CTYRAG 1 cut(s) 1475
SphI GCATGC 1 cut(s) 1584
Sse9I AATT 8 cut(s) 471, 912, 1046, 1064, 1124, 1185, 1251, 1714
SsiI CCGC 6 cut(s) 150, 666, 819, 960, 1042, 1490
SspI AATATT 1 cut(s) 593
SspMI CTAG 4 cut(s) 8, 12, 815, 1726
StyD4I CCNGG 3 cut(s) 976, 1116, 1495
StyI CCWWGG 3 cut(s) 954, 1007, 1056
TaaI ACNGT 8 cut(s) 221, 418, 650, 691, 845, 991, 1076, 1697
TaiI ACGT 2 cut(s) 213, 1428
TaqI TCGA 5 cut(s) 46, 93, 825, 1275, 1442
TasI AATT 8 cut(s) 471, 912, 1046, 1064, 1124, 1185, 1251, 1714
TatI WGTACW 2 cut(s) 765, 1722
TauI GCSGC 4 cut(s) 153, 821, 962, 1493
TfiI GAWTC 1 cut(s) 1702
Tru1I TTAA 2 cut(s) 420, 708
Tru9I TTAA 2 cut(s) 420, 708
TscAI CASTG 3 cut(s) 696, 793, 1570
TseFI GTSAC 3 cut(s) 797, 991, 1635
TseI GCWGC 9 cut(s) 110, 290, 677, 783, 1025, 1244, 1514, 1604, 1643
Tsp45I GTSAC 3 cut(s) 797, 991, 1635
TspDTI ATGAA 5 cut(s) 179, 209, 258, 1039, 1296
TspGWI ACGGA 2 cut(s) 856, 1563
TspRI CASTG 3 cut(s) 696, 793, 1570
Van91I CCANNNNNTGG 2 cut(s) 528, 1532
VpaK11BI GGWCC 2 cut(s) 512, 974
XapI RAATTY 1 cut(s) 471
XceI RCATGY 2 cut(s) 777, 1584
XcmI CCANNNNNNNNNTGG 2 cut(s) 525, 1063
XmiI GTMKAC 2 cut(s) 849, 1275
XspI CTAG 4 cut(s) 8, 12, 815, 1726
ZrmI AGTACT 1 cut(s) 1724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.