MD07G1206200.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28398867 .. 28402270
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1206200.v1.1.491

Sequence Viewer

Length: 2070 bp
ATGGCAGAGTTTGAAAGTGTCAAATTTTTTAAGATTTTGATTTTTGACGTTTATTTATCCAGCAATATTGCGCACGAATCTCTGCTTCTGCACAGCTACAAGAGAACCTTCAATGGCTTTGCTGCCAAGCTAACAGAGGAAGAAGCACAGAAGTTGGCTGGAATGGATGGTGTGGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCTCCAAACAACAAGGTCATGGGACTTCATTGGGTTTCCTAAAATAGTGAAGAGAAGCGCCATTGAAACTGATATCATTGTCGGGGTGATTGACTCTGGAATTTGGCCTGAATCTGCCAGCTTCAGTGACGCCGGGTTTGGTCCACCCCCCAAAAGGTGGAAAGGCGCATGCAAGGGCGAAGGCAATTTTACTTGCAACAATAAAATTATCGGAGCACGGTATTACCGCAGTCAACCCTACCCACAAAATAGCAGTGATATCTTGTCTCCGAGGGACACGGATGGCCATGGAACCCACACTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGGGTGCCATCAGCACGCATTGCGGTGTACAAAGTTTGTTGGTCAGATGGGTGCCCGGATGCTGATATTCTAGCGGCATTCGATGATGCCATAGCTGACGGTGTTGACATACTCTCTGTCTCCCTTGGGGGGTTTAAACCGTTAGATTATTTCAGAAATTCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTCACTTCCGCGTCTGCTGGCAATGAAGGGCCAAACCTGAAAACTATTACAAACTTTGCACCATGGTATCTTGCTGTGGCTGCTAGCACCATAGATCGCCACTTTGATACCAAGGGAATAGTAACAAACACATTTGAACTCAAGGGTAAATTCTACCCCATAGTATATGCTGCAGACGTTCCAGATACGGCAGCTGGTTACGATGGGGAAACATCCAGGTTATGCCGAATAGGCACGTTAGACAAAAAATTGGTGGAGGGTAAAATTGTTCTTTGTGATGGGCCTACTGGGTATGGGGCTATATATGCAGGCGCATTTGGTTATGTTTTGACAAGCCGAAAAGCAGCAGATGTAATCGATCCTGTTCCAATACCAGCAGCTTCCATTTGGTTCCACCTTGGTAACGAAATTGCCCATTACCTAAACTCAACCAGGAACCCAACCGCAACAATTTGGAAAAGTACTGAGGGTATGGATGCACTGGCACCATACGTACCTTCATTCTCATCAAGGGGTCCAAATCCAAACACTCCTAACATTCTCAAGCCAGATATCGCTTCTCCTGGAGTTTCCATTCTAGCAGCATGGTCTCCAATTGCCCCAGTTTCAGGTTTTGAAGGTGATGACAGGGTAACTTCATACAATATTATCTCGGGGACATCAATGGCATGCCCACATGCTGCGGGCGTGGCTGCATACGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTCTTACGACTACTGCCAAACCTATGAGTGCAAATCTTAACCCTGAAGCCGAATTCGCGTACGGTGCTGGCCTACTAAATCCTAGTAGGTCTCCGTATCCTGGTTTGGTATACGACGCTGTTGAAATCGATTACGTAAATTTTTTGTGTGCACAAGGCTATAGTACCAAATTATTGAAAGCTCTTACCGGGGATAGCTATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTCAAAGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCGTTCTAAAGTTCACATCTCTCGGGCAGAAGCTATCGTTTCAAGTCATGGTGAAAGGGTTGATTGAAAAAACCATAGTCTCTGGTTCTCTGGTGTGGGATGATGGTAAATTCCAAGTGAGGAGCCCCATTGTTGTGTATTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

690

Amino Acids

73.67

Weight (kDa)

8.34

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 24 - 69 5e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 92 - 531 2.4e-42 Subtilase family
fn3_6 PF17766 590 - 685 1.8e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1513
Acc16I TGCGCA 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 1513
AccB1I GGYRCC 3 cut(s) 580, 627, 1261
AccB7I CCANNNNNTGG 1 cut(s) 360
AccI GTMKAC 1 cut(s) 1632
AccII CGCG 2 cut(s) 788, 1580
AciI CCGC 6 cut(s) 430, 599, 650, 786, 1221, 1460
AclWI GGATC 2 cut(s) 1130, 1858
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 7 cut(s) 23, 303, 733, 926, 1574, 1660, 2027
AcuI CTGAAG 2 cut(s) 310, 1587
AcyI GRCGYC 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 1760
AfaI GTAC 5 cut(s) 605, 1240, 1272, 1583, 1687
AfiI CCNNNNNNNGG 5 cut(s) 357, 360, 705, 1385, 1622
AjnI CCWGG 5 cut(s) 992, 1208, 1339, 1621, 1891
AloI GAACNNNNNNTCC 2 cut(s) 1793, 1825
Alw21I GWGCWC 3 cut(s) 421, 1675, 1888
Alw26I GTCTC 5 cut(s) 474, 700, 1371, 1617, 2002
Alw44I GTGCAC 2 cut(s) 1671, 1884
AlwI GGATC 2 cut(s) 1130, 1858
Ama87I CYCGRG 2 cut(s) 1429, 1940
AoxI GGCC 5 cut(s) 308, 487, 806, 1058, 1591
ApaLI GTGCAC 2 cut(s) 1671, 1884
ApoI RAATTY 7 cut(s) 23, 303, 733, 926, 1574, 1660, 2027
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AspLEI GCGC 4 cut(s) 73, 263, 371, 1091
AspS9I GGNCC 5 cut(s) 344, 806, 1058, 1292, 1833
AsuC2I CCSGG 3 cut(s) 337, 632, 1711
AsuHPI GGTGA 6 cut(s) 301, 1409, 1481, 1494, 1845, 1981
AsuNHI GCTAGC 1 cut(s) 860
AvaI CYCGRG 2 cut(s) 1429, 1940
AvaII GGWCC 3 cut(s) 344, 1292, 1833
BaeGI GKGCMC 3 cut(s) 632, 1675, 1888
BaeI ACNNNNGTAYC 2 cut(s) 827, 860
BalI TGGCCA 1 cut(s) 489
BanI GGYRCC 3 cut(s) 580, 627, 1261
BanII GRGCYC 1 cut(s) 2045
Bbv12I GWGCWC 3 cut(s) 421, 1675, 1888
BccI CCATC 7 cut(s) 161, 479, 592, 617, 974, 1049, 2015
BceAI ACGGC 1 cut(s) 981
BciT130I CCWGG 5 cut(s) 994, 1210, 1341, 1623, 1893
BciVI GTATCC 1 cut(s) 1629
BclI TGATCA 1 cut(s) 1762
BcnI CCSGG 3 cut(s) 337, 632, 1711
BcoDI GTCTC 5 cut(s) 474, 700, 1371, 1617, 2002
BfaI CTAG 6 cut(s) 189, 647, 861, 1355, 1605, 1743
BfmI CTRYAG 3 cut(s) 948, 1681, 1720
BfoI RGCGCY 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 1513
BfuI GTATCC 1 cut(s) 1629
BglI GCCNNNNNGGC 1 cut(s) 1008
BmcAI AGTACT 1 cut(s) 1240
Bme1390I CCNGG 8 cut(s) 337, 632, 994, 1210, 1341, 1623, 1711, 1893
Bme18I GGWCC 3 cut(s) 344, 1292, 1833
BmeT110I CYCGRG 2 cut(s) 1429, 1940
BmgT120I GGNCC 5 cut(s) 344, 806, 1058, 1292, 1833
BmiI GGNNCC 8 cut(s) 496, 582, 629, 1169, 1214, 1263, 1293, 2042
BmrFI CCNGG 8 cut(s) 337, 632, 994, 1210, 1341, 1623, 1711, 1893
BmrI ACTGGG 2 cut(s) 1074, 1373
BmsI GCATC 4 cut(s) 515, 625, 652, 1243
BmtI GCTAGC 1 cut(s) 864
BmuI ACTGGG 2 cut(s) 1074, 1373
BpmI CTGGAG 1 cut(s) 1362
BpuEI CTTGAG 2 cut(s) 902, 1304
BpuMI CCSGG 3 cut(s) 337, 632, 1711
Bsa29I ATCGAT 2 cut(s) 1134, 1650
BsaAI YACGTR 2 cut(s) 1270, 1657
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 1371, 1617
BsaJI CCNNGG 7 cut(s) 473, 490, 700, 839, 888, 1174, 1710
Bsc4I CCNNNNNNNGG 5 cut(s) 357, 360, 705, 1385, 1622
Bse1I ACTGG 3 cut(s) 1069, 1263, 1379
Bse3DI GCAATG 2 cut(s) 594, 805
BseBI CCWGG 5 cut(s) 994, 1210, 1341, 1623, 1893
BseCI ATCGAT 2 cut(s) 1134, 1650
BseDI CCNNGG 7 cut(s) 473, 490, 700, 839, 888, 1174, 1710
BseGI GGATG 6 cut(s) 172, 490, 640, 989, 1258, 2023
BseLI CCNNNNNNNGG 5 cut(s) 357, 360, 705, 1385, 1622
BseMI GCAATG 2 cut(s) 594, 805
BseMII CTCAG 2 cut(s) 1233, 1771
BseNI ACTGG 3 cut(s) 1069, 1263, 1379
BseRI GAGGAG 2 cut(s) 588, 2053
BseSI GKGCMC 3 cut(s) 632, 1675, 1888
BsgI GTGCAG 1 cut(s) 74
Bsh1236I CGCG 2 cut(s) 788, 1580
BshFI GGCC 5 cut(s) 310, 489, 808, 1060, 1593
BshNI GGYRCC 3 cut(s) 580, 627, 1261
BshVI ATCGAT 2 cut(s) 1134, 1650
BsiHKAI GWGCWC 3 cut(s) 421, 1675, 1888
BsiHKCI CYCGRG 2 cut(s) 1429, 1940
BsiSI CCGG 3 cut(s) 336, 632, 1710
BsiWI CGTACG 1 cut(s) 1581
BslFI GGGAC 4 cut(s) 239, 491, 577, 1447
BslI CCNNNNNNNGG 5 cut(s) 357, 360, 705, 1385, 1622
BsmAI GTCTC 5 cut(s) 474, 700, 1371, 1617, 2002
BsmFI GGGAC 4 cut(s) 239, 491, 577, 1447
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 5 cut(s) 310, 489, 808, 1060, 1593
Bso31I GGTCTC 2 cut(s) 1371, 1617
BsoBI CYCGRG 2 cut(s) 1429, 1940
Bsp1286I GDGCHC 5 cut(s) 421, 632, 1675, 1888, 2045
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 4 cut(s) 871, 1135, 1762, 1850
Bsp19I CCATGG 2 cut(s) 490, 839
BspACI CCGC 6 cut(s) 430, 599, 650, 786, 1221, 1460
BspANI GGCC 5 cut(s) 310, 489, 808, 1060, 1593
BspCNI CTCAG 2 cut(s) 1234, 1770
BspDI ATCGAT 2 cut(s) 1134, 1650
BspFNI CGCG 2 cut(s) 788, 1580
BspLI GGNNCC 8 cut(s) 496, 582, 629, 1169, 1214, 1263, 1293, 2042
BspMAI CTGCAG 1 cut(s) 952
BspMI ACCTGC 1 cut(s) 1513
BspOI GCTAGC 1 cut(s) 864
BspPI GGATC 2 cut(s) 1130, 1858
BspT107I GGYRCC 3 cut(s) 580, 627, 1261
BspTNI GGTCTC 2 cut(s) 1371, 1617
BsrDI GCAATG 2 cut(s) 594, 805
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 3 cut(s) 1069, 1263, 1379
BssECI CCNNGG 7 cut(s) 473, 490, 700, 839, 888, 1174, 1710
BssMI GATC 4 cut(s) 871, 1135, 1762, 1850
BssNAI GTATAC 1 cut(s) 1633
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 5 cut(s) 490, 700, 839, 888, 1174
Bst1107I GTATAC 1 cut(s) 1633
Bst2UI CCWGG 5 cut(s) 994, 1210, 1341, 1623, 1893
Bst4CI ACNGT 5 cut(s) 423, 677, 717, 1586, 1837
Bst6I CTCTTC 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 596
BstAUI TGTACA 1 cut(s) 603
BstBAI YACGTR 2 cut(s) 1270, 1657
BstC8I GCNNGC 9 cut(s) 322, 373, 592, 796, 862, 1087, 1447, 1462, 1591
BstDEI CTNAG 4 cut(s) 198, 526, 1242, 1757
BstDSI CCRYGG 2 cut(s) 490, 839
BstEII GGTNACC 1 cut(s) 1500
BstF5I GGATG 6 cut(s) 172, 490, 640, 989, 1258, 2023
BstFNI CGCG 2 cut(s) 788, 1580
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 4 cut(s) 73, 263, 371, 1091
BstKTI GATC 4 cut(s) 874, 1138, 1765, 1853
BstMAI GTCTC 5 cut(s) 474, 700, 1371, 1617, 2002
BstMBI GATC 4 cut(s) 871, 1135, 1762, 1850
BstNI CCWGG 5 cut(s) 994, 1210, 1341, 1623, 1893
BstNSI RCATGY 3 cut(s) 375, 1449, 1457
BstPI GGTNACC 1 cut(s) 1500
BstSCI CCNGG 8 cut(s) 335, 630, 992, 1208, 1339, 1621, 1709, 1891
BstSFI CTRYAG 3 cut(s) 948, 1681, 1720
BstSLI GKGCMC 3 cut(s) 632, 1675, 1888
BstSNI TACGTA 2 cut(s) 1270, 1657
BstUI CGCG 2 cut(s) 788, 1580
BstZ17I GTATAC 1 cut(s) 1633
Bsu15I ATCGAT 2 cut(s) 1134, 1650
BsuI GTATCC 1 cut(s) 1629
BsuRI GGCC 5 cut(s) 310, 489, 808, 1060, 1593
BsuTUI ATCGAT 2 cut(s) 1134, 1650
BtgI CCRYGG 2 cut(s) 490, 839
BtsCI GGATG 6 cut(s) 172, 490, 640, 989, 1258, 2023
BtsI GCAGTG 2 cut(s) 463, 501
BtsIMutI CAGTG 5 cut(s) 334, 463, 501, 1256, 1765
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 9 cut(s) 322, 373, 592, 796, 862, 1087, 1447, 1462, 1591
CfoI GCGC 4 cut(s) 73, 263, 371, 1091
Cfr13I GGNCC 5 cut(s) 344, 806, 1058, 1292, 1833
ClaI ATCGAT 2 cut(s) 1134, 1650
CseI GACGC 3 cut(s) 341, 777, 1646
Csp6I GTAC 5 cut(s) 604, 1239, 1271, 1582, 1686
CviAII CATG 9 cut(s) 222, 372, 491, 840, 1362, 1446, 1454, 1748, 1966
CviQI GTAC 5 cut(s) 604, 1239, 1271, 1582, 1686
DdeI CTNAG 4 cut(s) 198, 526, 1242, 1757
DpnI GATC 4 cut(s) 873, 1137, 1764, 1852
DpnII GATC 4 cut(s) 871, 1135, 1762, 1850
DraI TTTAAA 1 cut(s) 712
DraIII CACNNNGTG 1 cut(s) 1760
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Eco105I TACGTA 2 cut(s) 1270, 1657
Eco130I CCWWGG 5 cut(s) 490, 700, 839, 888, 1174
Eco24I GRGCYC 1 cut(s) 2045
Eco31I GGTCTC 2 cut(s) 1371, 1617
Eco32I GATATC 3 cut(s) 277, 463, 1330
Eco47I GGWCC 3 cut(s) 344, 1292, 1833
Eco57I CTGAAG 2 cut(s) 310, 1587
Eco88I CYCGRG 2 cut(s) 1429, 1940
Eco91I GGTNACC 1 cut(s) 1500
EcoO65I GGTNACC 1 cut(s) 1500
EcoRI GAATTC 1 cut(s) 1574
EcoRII CCWGG 5 cut(s) 992, 1208, 1339, 1621, 1891
EcoRV GATATC 3 cut(s) 277, 463, 1330
EcoT14I CCWWGG 5 cut(s) 490, 700, 839, 888, 1174
EcoT38I GRGCYC 1 cut(s) 2045
ErhI CCWWGG 5 cut(s) 490, 700, 839, 888, 1174
FaeI CATG 9 cut(s) 225, 375, 494, 843, 1365, 1449, 1457, 1751, 1969
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 4 cut(s) 239, 491, 577, 1447
FatI CATG 9 cut(s) 221, 371, 490, 839, 1361, 1445, 1453, 1747, 1965
FauI CCCGC 1 cut(s) 1453
FbaI TGATCA 1 cut(s) 1762
FblI GTMKAC 1 cut(s) 1632
FokI GGATG 6 cut(s) 179, 497, 647, 976, 1265, 2030
FriOI GRGCYC 1 cut(s) 2045
FspBI CTAG 6 cut(s) 189, 647, 861, 1355, 1605, 1743
FspI TGCGCA 1 cut(s) 72
GlaI GCGC 4 cut(s) 72, 262, 370, 1090
GsuI CTGGAG 1 cut(s) 1362
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 5 cut(s) 310, 489, 808, 1060, 1593
HapII CCGG 3 cut(s) 336, 632, 1710
HgaI GACGC 3 cut(s) 341, 777, 1646
HhaI GCGC 4 cut(s) 73, 263, 371, 1091
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 9 cut(s) 225, 375, 494, 843, 1365, 1449, 1457, 1751, 1969
Hin6I GCGC 4 cut(s) 71, 261, 369, 1089
HinP1I GCGC 4 cut(s) 71, 261, 369, 1089
HincII GTYRAC 2 cut(s) 437, 682
HindII GTYRAC 2 cut(s) 437, 682
HinfI GANTC 3 cut(s) 77, 296, 314
HpaII CCGG 3 cut(s) 336, 632, 1710
HphI GGTGA 6 cut(s) 301, 1409, 1481, 1494, 1845, 1981
Hpy166II GTNNAC 9 cut(s) 347, 437, 604, 682, 1633, 1673, 1863, 1886, 1932
Hpy188I TCNGA 4 cut(s) 416, 474, 622, 731
Hpy188III TCNNGA 2 cut(s) 300, 959
Hpy8I GTNNAC 9 cut(s) 347, 437, 604, 682, 1633, 1673, 1863, 1886, 1932
Hpy99I CGWCG 1 cut(s) 1640
HpyAV CCTTC 6 cut(s) 118, 377, 797, 1284, 1388, 1788
HpyCH4III ACNGT 5 cut(s) 423, 677, 717, 1586, 1837
HpyCH4IV ACGT 6 cut(s) 48, 954, 1013, 1269, 1476, 1656
HpyF3I CTNAG 4 cut(s) 198, 526, 1242, 1757
HpySE526I ACGT 6 cut(s) 48, 954, 1013, 1269, 1476, 1656
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 9 cut(s) 225, 375, 494, 843, 1365, 1449, 1457, 1751, 1969
HspAI GCGC 4 cut(s) 71, 261, 369, 1089
Ksp22I TGATCA 1 cut(s) 1762
Kzo9I GATC 4 cut(s) 871, 1135, 1762, 1850
LmnI GCTCC 4 cut(s) 210, 416, 749, 2040
LweI GCATC 4 cut(s) 515, 625, 652, 1243
MaeI CTAG 6 cut(s) 189, 647, 861, 1355, 1605, 1743
MaeII ACGT 6 cut(s) 48, 954, 1013, 1269, 1476, 1656
MaeIII GTNAC 7 cut(s) 329, 898, 974, 1178, 1408, 1500, 1837
MalI GATC 4 cut(s) 873, 1137, 1764, 1852
MboI GATC 4 cut(s) 871, 1135, 1762, 1850
MboII GAAGA 2 cut(s) 152, 265
MfeI CAATTG 2 cut(s) 1371, 1495
MhlI GDGCHC 5 cut(s) 421, 632, 1675, 1888, 2045
MlsI TGGCCA 1 cut(s) 489
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 1828
MnlI CCTC 8 cut(s) 130, 468, 566, 569, 1027, 1237, 1807, 2031
Mox20I TGGCCA 1 cut(s) 489
MscI TGGCCA 1 cut(s) 489
MseI TTAA 5 cut(s) 30, 711, 764, 1560, 1911
MslI CAYNNNNRTG 2 cut(s) 599, 2051
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 3 cut(s) 336, 632, 1710
MspR9I CCNGG 8 cut(s) 337, 632, 994, 1210, 1341, 1623, 1711, 1893
MssI GTTTAAAC 1 cut(s) 712
MunI CAATTG 2 cut(s) 1371, 1495
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 5 cut(s) 994, 1210, 1341, 1623, 1893
MvnI CGCG 2 cut(s) 788, 1580
NciI CCSGG 3 cut(s) 337, 632, 1711
NcoI CCATGG 2 cut(s) 490, 839
NdeII GATC 4 cut(s) 871, 1135, 1762, 1850
NheI GCTAGC 1 cut(s) 860
NlaIII CATG 9 cut(s) 225, 375, 494, 843, 1365, 1449, 1457, 1751, 1969
NlaIV GGNNCC 8 cut(s) 496, 582, 629, 1169, 1214, 1263, 1293, 2042
NmuCI GTSAC 3 cut(s) 329, 1500, 1837
NsbI TGCGCA 1 cut(s) 72
NspI RCATGY 3 cut(s) 375, 1449, 1457
PaeI GCATGC 2 cut(s) 375, 1449
PaqCI CACCTGC 1 cut(s) 1513
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 2 cut(s) 77, 314
Pfl23II CGTACG 1 cut(s) 1581
PflMI CCANNNNNTGG 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 1339
PleI GAGTC 1 cut(s) 290
PmeI GTTTAAAC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 290
Ppu21I YACGTR 2 cut(s) 1270, 1657
Psp6I CCWGG 5 cut(s) 992, 1208, 1339, 1621, 1891
PspEI GGTNACC 1 cut(s) 1500
PspGI CCWGG 5 cut(s) 992, 1208, 1339, 1621, 1891
PspLI CGTACG 1 cut(s) 1581
PspN4I GGNNCC 8 cut(s) 496, 582, 629, 1169, 1214, 1263, 1293, 2042
PspPI GGNCC 5 cut(s) 344, 806, 1058, 1292, 1833
PstI CTGCAG 1 cut(s) 952
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 5 cut(s) 605, 1240, 1272, 1583, 1687
RsaNI GTAC 5 cut(s) 604, 1239, 1271, 1582, 1686
RseI CAYNNNNRTG 2 cut(s) 599, 2051
SaqAI TTAA 5 cut(s) 30, 711, 764, 1560, 1911
Sau3AI GATC 4 cut(s) 871, 1135, 1762, 1850
Sau96I GGNCC 5 cut(s) 344, 806, 1058, 1292, 1833
ScaI AGTACT 1 cut(s) 1240
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 8 cut(s) 337, 632, 994, 1210, 1341, 1623, 1711, 1893
SduI GDGCHC 5 cut(s) 421, 632, 1675, 1888, 2045
SfaNI GCATC 4 cut(s) 515, 625, 652, 1243
SfcI CTRYAG 3 cut(s) 948, 1681, 1720
SinI GGWCC 3 cut(s) 344, 1292, 1833
SmiMI CAYNNNNRTG 2 cut(s) 599, 2051
SmlI CTYRAG 2 cut(s) 917, 1319
SmoI CTYRAG 2 cut(s) 917, 1319
SnaBI TACGTA 2 cut(s) 1270, 1657
SphI GCATGC 2 cut(s) 375, 1449
SsiI CCGC 6 cut(s) 430, 599, 650, 786, 1221, 1460
SspI AATATT 3 cut(s) 67, 777, 1423
SspMI CTAG 6 cut(s) 189, 647, 861, 1355, 1605, 1743
StyD4I CCNGG 8 cut(s) 335, 630, 992, 1208, 1339, 1621, 1709, 1891
StyI CCWWGG 5 cut(s) 490, 700, 839, 888, 1174
TaaI ACNGT 5 cut(s) 423, 677, 717, 1586, 1837
TaiI ACGT 6 cut(s) 51, 957, 1016, 1272, 1479, 1659
TaqI TCGA 4 cut(s) 657, 1134, 1650, 1799
TatI WGTACW 2 cut(s) 603, 1238
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 2 cut(s) 77, 314
Tru1I TTAA 5 cut(s) 30, 711, 764, 1560, 1911
Tru9I TTAA 5 cut(s) 30, 711, 764, 1560, 1911
TscAI CASTG 5 cut(s) 334, 463, 508, 1263, 1765
TseFI GTSAC 3 cut(s) 329, 1500, 1837
Tsp45I GTSAC 3 cut(s) 329, 1500, 1837
TspDTI ATGAA 4 cut(s) 220, 816, 1266, 1404
TspGWI ACGGA 3 cut(s) 497, 1605, 1801
TspRI CASTG 5 cut(s) 334, 463, 508, 1263, 1765
Van91I CCANNNNNTGG 1 cut(s) 360
VneI GTGCAC 2 cut(s) 1671, 1884
VpaK11BI GGWCC 3 cut(s) 344, 1292, 1833
XapI RAATTY 7 cut(s) 23, 303, 733, 926, 1574, 1660, 2027
XceI RCATGY 3 cut(s) 375, 1449, 1457
XmiI GTMKAC 1 cut(s) 1632
XspI CTAG 6 cut(s) 189, 647, 861, 1355, 1605, 1743
ZrmI AGTACT 1 cut(s) 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.